Rh2CG213200

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000319
Physical Location & Seq
Forward (+)
20966459 .. 20968956
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG213200.1

Sequence Viewer

Length: 993 bp
ATGTTAGACATCGTGACGATGATGGTGTCCAAAAATCCTGAAATAATCAAAGAAGTTGATGAACTTGGTTGGACTCCCTTACACTATGCAGCATTCAGAGGGAATGTATCGGCAATTAGATCGCTGATAAAACGTAACAGTTCTGTAGCTTACATATTAGACAACTGTGGAATGTCTGCTCTTCATGTTGCAGCGTACGGAGGCCACATAAAAGTAATGAAAGAATTGATTCGATTGCGGCCTGATACTTGTGATCTGCTCAACCACAAAGACCAAACGGCTGTACATGCTGCAGTTTTGGGTGAGAAGCCGGCTGTTATTAGGTATATTTTGAAGACGCCTACTCTAGCAGGACTTGTAAACGAAGCAGACGATGATGGAAATACTCCTTTACATCTGGCTGCCTCTATACCGAATTCTGCAATTATGAAAGCCTTGGCAAGGGATTGCAAAGTTGACAAGACTGCAACCAATGTTCACCACTCCAAAGCGGTTGATATATACCTTGGTAACAATATTGAACTGTACACCATTGGTAGACGAAGCGTCTTGCATAATTTGGGGAGGTCCATCGGTGTGCCAATTTTCCAGCGATATGTGCGCCGTGATTTCATGAAATTGGAATCATCGGAGGAGAAGAATGACTTCATGAAATTAGAATCATCGAAGGAGAAGAAGGCTACATCCACCACAGTGACCATTGCAAACGAAAAATCAGTCAAAGCTTCGCTGGATGACTCGAAAAGATTTGACACAAATCTACTAGTTGCGAAAGACCAGGTTGGCCGTTCTGCCAACGGTGCGCGAAATGAGGACACGCGATTGGATCCGTTCCCGCATAATTTTAATGGGGGGAAGTTCCTCCAACAACTCTATTTTGGTGCTTTCTTTTCTAAGCTTAAACATATGTGTACGCATACCACAGTACGTCCTCGGTACCCTGTATCGCCAGTATCAGAGACAATGTCCCAGGATCATTGTAACCCACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

330

Amino Acids

36.76

Weight (kDa)

9.14

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 3 - 78 8.8e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 85 - 156 4.1e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 936
AccB1I GGYRCC 1 cut(s) 936
AccI GTMKAC 1 cut(s) 538
AccII CGCG 2 cut(s) 805, 820
AciI CCGC 3 cut(s) 238, 491, 836
AclWI GGATC 3 cut(s) 821, 834, 981
AcoI YGGCCR 1 cut(s) 784
AcsI RAATTY 1 cut(s) 415
AcyI GRCGYC 1 cut(s) 338
AfaI GTAC 6 cut(s) 197, 285, 527, 913, 927, 938
AfiI CCNNNNNNNGG 1 cut(s) 441
AgsI TTSAA 2 cut(s) 334, 521
AhdI GACNNNNNGTC 1 cut(s) 545
AhlI ACTAGT 1 cut(s) 763
AjnI CCWGG 2 cut(s) 777, 969
AleI CACNNNNGTG 1 cut(s) 692
AluBI AGCT 3 cut(s) 149, 725, 898
AluI AGCT 3 cut(s) 149, 725, 898
Alw26I GTCTC 1 cut(s) 953
AlwI GGATC 3 cut(s) 821, 834, 981
AoxI GGCC 3 cut(s) 202, 239, 784
ApeKI GCWGC 4 cut(s) 89, 191, 290, 401
ApoI RAATTY 1 cut(s) 415
ArsI GACNNNNNNTTYG 2 cut(s) 702, 734
Asp700I GAANNNNTTC 2 cut(s) 228, 644
Asp718I GGTACC 1 cut(s) 936
AspLEI GCGC 2 cut(s) 603, 805
AspS9I GGNCC 1 cut(s) 567
AsuHPI GGTGA 2 cut(s) 314, 470
AvaII GGWCC 1 cut(s) 567
BamHI GGATCC 1 cut(s) 826
BanI GGYRCC 1 cut(s) 936
BbsI GAAGAC 1 cut(s) 341
BbvI GCAGC 4 cut(s) 101, 203, 277, 388
BccI CCATC 3 cut(s) 16, 371, 578
BceAI ACGGC 3 cut(s) 294, 588, 771
BcgI CGANNNNNNTGC 2 cut(s) 102, 136
BciT130I CCWGG 2 cut(s) 779, 971
BcoDI GTCTC 1 cut(s) 953
BcuI ACTAGT 1 cut(s) 763
BfaI CTAG 2 cut(s) 347, 764
BfmI CTRYAG 2 cut(s) 144, 291
BisI GCNGC 5 cut(s) 90, 192, 239, 291, 402
BlsI GCNGC 5 cut(s) 91, 193, 240, 292, 403
Bme1390I CCNGG 2 cut(s) 779, 971
Bme18I GGWCC 1 cut(s) 567
BmeRI GACNNNNNGTC 1 cut(s) 545
BmgT120I GGNCC 1 cut(s) 567
BmiI GGNNCC 2 cut(s) 828, 938
BmrFI CCNGG 2 cut(s) 779, 971
BpiI GAAGAC 1 cut(s) 341
BsaHI GRCGYC 1 cut(s) 338
BsaJI CCNNGG 4 cut(s) 435, 505, 932, 969
Bsc4I CCNNNNNNNGG 1 cut(s) 441
Bse118I RCCGGY 1 cut(s) 310
Bse1I ACTGG 1 cut(s) 950
Bse3DI GCAATG 1 cut(s) 699
BseBI CCWGG 2 cut(s) 779, 971
BseDI CCNNGG 4 cut(s) 435, 505, 932, 969
BseGI GGATG 2 cut(s) 683, 739
BseLI CCNNNNNNNGG 1 cut(s) 441
BseMI GCAATG 1 cut(s) 699
BseNI ACTGG 1 cut(s) 950
BseRI GAGGAG 1 cut(s) 647
BseXI GCAGC 4 cut(s) 101, 203, 277, 388
Bsh1236I CGCG 2 cut(s) 805, 820
BshFI GGCC 3 cut(s) 204, 241, 786
BshNI GGYRCC 1 cut(s) 936
BsiSI CCGG 1 cut(s) 311
BsiWI CGTACG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 952
BslI CCNNNNNNNGG 1 cut(s) 441
BsmAI GTCTC 1 cut(s) 953
BsmFI GGGAC 1 cut(s) 952
BsmI GAATGC 1 cut(s) 92
BsnI GGCC 3 cut(s) 204, 241, 786
Bsp1407I TGTACA 2 cut(s) 283, 525
Bsp143I GATC 4 cut(s) 119, 253, 826, 973
BspACI CCGC 3 cut(s) 238, 491, 836
BspANI GGCC 3 cut(s) 204, 241, 786
BspFNI CGCG 2 cut(s) 805, 820
BspHI TCATGA 2 cut(s) 612, 648
BspLI GGNNCC 2 cut(s) 828, 938
BspMAI CTGCAG 1 cut(s) 295
BspPI GGATC 3 cut(s) 821, 834, 981
BspQI GCTCTTC 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 936
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 1 cut(s) 310
BsrGI TGTACA 2 cut(s) 283, 525
BsrI ACTGG 1 cut(s) 950
BssAI RCCGGY 1 cut(s) 310
BssECI CCNNGG 4 cut(s) 435, 505, 932, 969
BssMI GATC 4 cut(s) 119, 253, 826, 973
BssNI GRCGYC 1 cut(s) 338
BssT1I CCWWGG 2 cut(s) 435, 505
Bst2UI CCWGG 2 cut(s) 779, 971
Bst4CI ACNGT 6 cut(s) 140, 167, 525, 694, 800, 925
Bst6I CTCTTC 1 cut(s) 186
BstACI GRCGYC 1 cut(s) 338
BstAUI TGTACA 2 cut(s) 283, 525
BstC8I GCNNGC 1 cut(s) 312
BstDEI CTNAG 1 cut(s) 894
BstENI CCTNNNNNAGG 1 cut(s) 439
BstF5I GGATG 2 cut(s) 683, 739
BstFNI CGCG 2 cut(s) 805, 820
BstHHI GCGC 2 cut(s) 603, 805
BstKTI GATC 4 cut(s) 122, 256, 829, 976
BstMAI GTCTC 1 cut(s) 953
BstMBI GATC 4 cut(s) 119, 253, 826, 973
BstMWI GCNNNNNNNGC 3 cut(s) 287, 598, 800
BstNI CCWGG 2 cut(s) 779, 971
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 2 cut(s) 777, 969
BstSFI CTRYAG 2 cut(s) 144, 291
BstUI CGCG 2 cut(s) 805, 820
BstV1I GCAGC 4 cut(s) 101, 203, 277, 388
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 1 cut(s) 826
BstYI RGATCY 1 cut(s) 826
BsuRI GGCC 3 cut(s) 204, 241, 786
BtsCI GGATG 2 cut(s) 683, 739
BtsIMutI CAGTG 1 cut(s) 699
Cac8I GCNNGC 1 cut(s) 312
CciI TCATGA 2 cut(s) 612, 648
CfoI GCGC 2 cut(s) 603, 805
Cfr10I RCCGGY 1 cut(s) 310
Cfr13I GGNCC 1 cut(s) 567
CseI GACGC 2 cut(s) 346, 535
CsiI ACCWGGT 1 cut(s) 777
Csp6I GTAC 6 cut(s) 196, 284, 526, 912, 926, 937
CviAII CATG 4 cut(s) 185, 287, 613, 649
CviQI GTAC 6 cut(s) 196, 284, 526, 912, 926, 937
DdeI CTNAG 1 cut(s) 894
DpnI GATC 4 cut(s) 121, 255, 828, 975
DpnII GATC 4 cut(s) 119, 253, 826, 973
DriI GACNNNNNGTC 1 cut(s) 545
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 1 cut(s) 186
Eam1105I GACNNNNNGTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 186
Eco130I CCWWGG 2 cut(s) 435, 505
Eco47I GGWCC 1 cut(s) 567
EcoNI CCTNNNNNAGG 1 cut(s) 439
EcoRI GAATTC 1 cut(s) 415
EcoRII CCWGG 2 cut(s) 777, 969
EcoT14I CCWWGG 2 cut(s) 435, 505
ErhI CCWWGG 2 cut(s) 435, 505
FaeI CATG 4 cut(s) 188, 290, 616, 652
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FaqI GGGAC 1 cut(s) 952
FatI CATG 4 cut(s) 184, 286, 612, 648
FauI CCCGC 1 cut(s) 843
FauNDI CATATG 1 cut(s) 906
FblI GTMKAC 1 cut(s) 538
Fnu4HI GCNGC 5 cut(s) 90, 192, 239, 291, 402
FokI GGATG 2 cut(s) 670, 746
Fsp4HI GCNGC 5 cut(s) 90, 192, 239, 291, 402
FspBI CTAG 2 cut(s) 347, 764
GlaI GCGC 2 cut(s) 602, 804
GluI GCNGC 5 cut(s) 90, 192, 239, 291, 402
HaeIII GGCC 3 cut(s) 204, 241, 786
HapII CCGG 1 cut(s) 311
HgaI GACGC 2 cut(s) 346, 535
HhaI GCGC 2 cut(s) 603, 805
Hin1I GRCGYC 1 cut(s) 338
Hin1II CATG 4 cut(s) 188, 290, 616, 652
Hin6I GCGC 2 cut(s) 601, 803
HinP1I GCGC 2 cut(s) 601, 803
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 2 cut(s) 723, 896
HinfI GANTC 5 cut(s) 73, 229, 623, 659, 737
HpaII CCGG 1 cut(s) 311
HphI GGTGA 2 cut(s) 314, 470
Hpy166II GTNNAC 6 cut(s) 361, 457, 478, 528, 539, 912
Hpy188I TCNGA 3 cut(s) 98, 631, 958
Hpy188III TCNNGA 4 cut(s) 13, 38, 613, 649
Hpy8I GTNNAC 6 cut(s) 361, 457, 478, 528, 539, 912
HpyAV CCTTC 2 cut(s) 661, 670
HpyCH4III ACNGT 6 cut(s) 140, 167, 525, 694, 800, 925
HpyCH4IV ACGT 2 cut(s) 133, 928
HpyCH4V TGCA 8 cut(s) 89, 191, 293, 422, 450, 467, 553, 704
HpyF10VI GCNNNNNNNGC 3 cut(s) 287, 598, 800
HpyF3I CTNAG 1 cut(s) 894
HpySE526I ACGT 2 cut(s) 133, 928
Hsp92I GRCGYC 1 cut(s) 338
Hsp92II CATG 4 cut(s) 188, 290, 616, 652
HspAI GCGC 2 cut(s) 601, 803
KpnI GGTACC 1 cut(s) 940
KroI GCCGGC 1 cut(s) 310
KroNI GCCGGC 1 cut(s) 312
Kzo9I GATC 4 cut(s) 119, 253, 826, 973
LguI GCTCTTC 1 cut(s) 186
Lsp1109I GCAGC 4 cut(s) 101, 203, 277, 388
MabI ACCWGGT 1 cut(s) 777
MaeI CTAG 2 cut(s) 347, 764
MaeII ACGT 2 cut(s) 133, 928
MaeIII GTNAC 5 cut(s) 13, 134, 509, 694, 980
MalI GATC 4 cut(s) 121, 255, 828, 975
MboI GATC 4 cut(s) 119, 253, 826, 973
MboII GAAGA 4 cut(s) 173, 346, 649, 685
MflI RGATCY 1 cut(s) 826
MluCI AATT 9 cut(s) 114, 224, 415, 423, 556, 582, 617, 653, 841
MlyI GAGTC 2 cut(s) 67, 731
MmeI TCCRAC 2 cut(s) 50, 889
MnlI CCTC 8 cut(s) 92, 194, 415, 558, 625, 805, 872, 942
MroNI GCCGGC 1 cut(s) 310
MroXI GAANNNNTTC 2 cut(s) 228, 644
MseI TTAA 2 cut(s) 846, 900
MslI CAYNNNNRTG 2 cut(s) 575, 692
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 2 cut(s) 779, 971
Mva1269I GAATGC 1 cut(s) 92
MvaI CCWGG 2 cut(s) 779, 971
MvnI CGCG 2 cut(s) 805, 820
MwoI GCNNNNNNNGC 3 cut(s) 287, 598, 800
NaeI GCCGGC 1 cut(s) 312
NdeI CATATG 1 cut(s) 906
NdeII GATC 4 cut(s) 119, 253, 826, 973
NgoMIV GCCGGC 1 cut(s) 310
NlaIII CATG 4 cut(s) 188, 290, 616, 652
NlaIV GGNNCC 2 cut(s) 828, 938
NmuCI GTSAC 2 cut(s) 13, 694
NspI RCATGY 1 cut(s) 290
OliI CACNNNNGTG 1 cut(s) 692
PagI TCATGA 2 cut(s) 612, 648
PciSI GCTCTTC 1 cut(s) 186
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PctI GAATGC 1 cut(s) 92
PdiI GCCGGC 1 cut(s) 312
PdmI GAANNNNTTC 2 cut(s) 228, 644
PfeI GAWTC 3 cut(s) 229, 623, 659
Pfl23II CGTACG 1 cut(s) 195
PflFI GACNNNGTC 1 cut(s) 964
PkrI GCNGC 5 cut(s) 91, 193, 240, 292, 403
PleI GAGTC 2 cut(s) 67, 731
PpsI GAGTC 2 cut(s) 67, 731
Psp6I CCWGG 2 cut(s) 777, 969
PspGI CCWGG 2 cut(s) 777, 969
PspLI CGTACG 1 cut(s) 195
PspN4I GGNNCC 2 cut(s) 828, 938
PspPI GGNCC 1 cut(s) 567
PstI CTGCAG 1 cut(s) 295
PsuI RGATCY 1 cut(s) 826
PsyI GACNNNGTC 1 cut(s) 964
RsaI GTAC 6 cut(s) 197, 285, 527, 913, 927, 938
RsaNI GTAC 6 cut(s) 196, 284, 526, 912, 926, 937
RseI CAYNNNNRTG 2 cut(s) 575, 692
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 2 cut(s) 846, 900
SatI GCNGC 5 cut(s) 90, 192, 239, 291, 402
Sau3AI GATC 4 cut(s) 119, 253, 826, 973
Sau96I GGNCC 1 cut(s) 567
SchI GAGTC 2 cut(s) 67, 731
ScrFI CCNGG 2 cut(s) 779, 971
SetI ASST 9 cut(s) 136, 151, 326, 507, 569, 727, 783, 900, 931
SexAI ACCWGGT 1 cut(s) 777
SfcI CTRYAG 2 cut(s) 144, 291
SinI GGWCC 1 cut(s) 567
SmiMI CAYNNNNRTG 2 cut(s) 575, 692
SpeI ACTAGT 1 cut(s) 763
Sse9I AATT 9 cut(s) 114, 224, 415, 423, 556, 582, 617, 653, 841
SsiI CCGC 3 cut(s) 238, 491, 836
SspI AATATT 1 cut(s) 517
SspMI CTAG 2 cut(s) 347, 764
StyD4I CCNGG 2 cut(s) 777, 969
StyI CCWWGG 2 cut(s) 435, 505
TaaI ACNGT 6 cut(s) 140, 167, 525, 694, 800, 925
TaiI ACGT 2 cut(s) 136, 931
TaqI TCGA 3 cut(s) 232, 665, 740
TasI AATT 9 cut(s) 114, 224, 415, 423, 556, 582, 617, 653, 841
TatI WGTACW 2 cut(s) 283, 525
TauI GCSGC 1 cut(s) 241
TfiI GAWTC 3 cut(s) 229, 623, 659
Tru1I TTAA 2 cut(s) 846, 900
Tru9I TTAA 2 cut(s) 846, 900
TscAI CASTG 1 cut(s) 699
TseFI GTSAC 2 cut(s) 13, 694
TseI GCWGC 4 cut(s) 89, 191, 290, 401
Tsp45I GTSAC 2 cut(s) 13, 694
TspDTI ATGAA 8 cut(s) 75, 173, 233, 443, 601, 629, 637, 665
TspGWI ACGGA 2 cut(s) 213, 819
TspRI CASTG 1 cut(s) 699
Tth111I GACNNNGTC 1 cut(s) 964
VpaK11BI GGWCC 1 cut(s) 567
XagI CCTNNNNNAGG 1 cut(s) 439
XapI RAATTY 1 cut(s) 415
XceI RCATGY 1 cut(s) 290
XmiI GTMKAC 1 cut(s) 538
XmnI GAANNNNTTC 2 cut(s) 228, 644
XspI CTAG 2 cut(s) 347, 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.