MD05G1213400.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000319
Physical Location & Seq
Reverse (-)
34472346 .. 34474734
2389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1213400.v1.1.491

Sequence Viewer

Length: 1842 bp
ATGAATCCTTCTCTGTATGAAGCAGCTGCAACTGGTGATGTTGGCTTCTTGGAAGGACTTCTAACAGCTGATCTCTCTCAGAAAACACCTAAAGAGAACAATATTCTTCACATTGCAGCTGAATTCAAGCAAATAAACTTCTTCAAAAAAGTGAAAAAAAATCCCGAATCTCCCCGGTTTTGGGCCACCAACAAGAATGGCGAAACTCCTCTACATGTTGCTGCGAGAGTAGGTTGCGGTGAAGTAGTAACGTTCCTCATTGAGCACACAAAAGCGGAACCTATGGAAGTGGTTGATCTTGATCCAGAAAAGGCACCAACTGACGGTGAAGCTTACAAAAAACTACTCCGAATGCCGAATTTGGAAATGGATACAGCACTGCATGTTGCTGTTCGATACAATCATGCTGGAGTAGCGAAGATTTTGCTGAGTGCTGATCCTGAATTGTGTTGTTCATTTTACAGCACCAGGGAGTCACCCCTGTTCCTAGCTGTTAGAGCCGGGTCTACCAGCATTGCTGATTATATTTTAAAAAAGACTCCGGATCATGAATCTCTTTCTTTTCAGGGAACAAATGGTGTGACCGCTTTGCACGCAGCAGCCACTCGTATACCCTTCGCCAAGAAAGGAATTGTGCAATCTATGGTTACCAAAAATCCTGGGATGGTCGAAGTTCGTGATGAAATAGGGTGGACTCCATTACACTACGCATCACTGAGAGGGAACCTTAAAGCAGCTCAACTACTGATTCAAAATTGCAAAACTTTCAAATTTGCTTGTTACATCAAAGACGAGCTTGGCATGTCAGCTCTCCACGTCGCGGCATATGCAGGGCACACCCAAATAATTGAAGAGTTGATCGGACGTTGTCCTGATATTTGTGATTGTGTCAATCACAAGGGCCAAACAGCTCTACATGCAGCAGTTTTAGGCGAAAAGATAAATGTTGTGAAGTACGTTTTGAAGACGCCTAGGCTTGCGAGACTTATAAACGAAGCAGATAATGATGGAAACACTCCTTGGCATCTAGCTGCTATTCATGAAAATAGTGAAATCGTTGCTATTTTAAGGAGAGACAGGAGGTTGAACAGAACTGCTATCAATAAAGAATTTTTGCAAGTTAGCGACATTTTGCTCGCCGAGAACACGGGAAGAAAGGAAATCGTAAAAAGTCGCAATGTTTTGAACTGCCTGGCAAGAGACGTTGTCGTTACCGTGCCATATTATCAACAAAAAATCAAGCAGGAGTTCAAGAAATTTGGATCCCAAGGGGAAAGCAGTACGTCTGAGACACTGGTCAATACTACAAATACGAGAGAAAACTTGCAGGCTTATTTGGCTCAGACCTTCAAAAGAAATGATTCGAAACTTCTGGTAGCGACGGTCATTGCAACCGTTACATTTACGGCCGTTTTGAACCCGCCAGGAGGGTTTAAAGACGATGGAACACCAGTTTTAACAAAAGAAGGAGCTTACAAAGTATTTATGTTTTTTAACACGCTTTGCTTCGCTCTCTCAGTGTCGGCGATATTTAATGAATCCAGTCCTATATCTGTCCTGTCAACCCATCTACCCACACCTACGAGTCTTATTAGTTATTCCATTGCTGGCATGTTGGTGGCATTTTGTGCAGCCCTGATAGCAGTGGAGCCCAAGCGGCCCGGGCAAAGTATGGTTAACTATTTTTTTTTTGGCAACGATGGTTCCGTTATTGCTTCTCTGCCATCTCGTATTGGTGCCGTAATTGTAGGCGTAGTAGTTTGCATCCCTATTTTTAACTCTCTAAGTCAAATACTCAACGGGGAAACAAGGATCCACGACCTCAAGAAGCACATAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

614

Amino Acids

67.3

Weight (kDa)

8.65

Isoelectric Point (pI)

31.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 93 7.8e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 113 - 182 8.6e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 127 - 201 9.8e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 161 - 252 5.7e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 207 - 288 3.9e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 262 - 322 1.2e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 271 - 357 2.9e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 271 - 320 1.8e-07 Ankyrin repeats (many copies)
PGG PF13962 451 - 548 7.5e-20 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 989
AccB1I GGYRCC 2 cut(s) 313, 1736
AccI GTMKAC 2 cut(s) 506, 610
AccII CGCG 1 cut(s) 821
AccIII TCCGGA 1 cut(s) 541
AciI CCGC 6 cut(s) 237, 275, 585, 821, 1421, 1657
AclI AACGTT 1 cut(s) 251
AclWI GGATC 7 cut(s) 296, 431, 552, 1257, 1270, 1807, 1820
AcoI YGGCCR 1 cut(s) 1407
AcsI RAATTY 5 cut(s) 122, 358, 770, 1109, 1256
AcyI GRCGYC 1 cut(s) 968
AfaI GTAC 2 cut(s) 956, 1282
AfiI CCNNNNNNNGG 5 cut(s) 180, 181, 323, 820, 1427
AflIII ACRYGT 1 cut(s) 214
AjiI CACGTC 1 cut(s) 817
AjnI CCWGG 4 cut(s) 467, 658, 1191, 1423
AjuI GAANNNNNNNTTGG 2 cut(s) 1003, 1035
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 4 cut(s) 976, 1068, 1194, 1283
AlwI GGATC 7 cut(s) 296, 431, 552, 1257, 1270, 1807, 1820
Ama87I CYCGRG 1 cut(s) 1661
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 4 cut(s) 183, 901, 1407, 1658
ApoI RAATTY 5 cut(s) 122, 358, 770, 1109, 1256
Asp700I GAANNNNTTC 2 cut(s) 57, 1360
AspA2I CCTAGG 1 cut(s) 971
AspS9I GGNCC 3 cut(s) 183, 901, 1659
AsuC2I CCSGG 4 cut(s) 175, 502, 1662, 1663
AsuHPI GGTGA 4 cut(s) 47, 251, 338, 468
AsuII TTCGAA 1 cut(s) 1364
AvaI CYCGRG 1 cut(s) 1661
AvrII CCTAGG 1 cut(s) 971
BaeGI GKGCMC 1 cut(s) 837
BamHI GGATCC 2 cut(s) 1262, 1812
BanI GGYRCC 2 cut(s) 313, 1736
BanII GRGCYC 1 cut(s) 1653
BbsI GAAGAC 1 cut(s) 971
Bbv12I GWGCWC 1 cut(s) 267
BccI CCATC 6 cut(s) 658, 1001, 1436, 1575, 1694, 1732
BceAI ACGGC 3 cut(s) 1394, 1422, 1724
BcgI CGANNNNNNTGC 2 cut(s) 406, 440
BciT130I CCWGG 4 cut(s) 469, 660, 1193, 1425
BciVI GTATCC 1 cut(s) 364
BcnI CCSGG 4 cut(s) 175, 502, 1662, 1663
BcoDI GTCTC 4 cut(s) 976, 1068, 1194, 1283
BfaI CTAG 3 cut(s) 488, 972, 1028
BfuI GTATCC 1 cut(s) 364
BglI GCCNNNNNGGC 1 cut(s) 1657
BlnI CCTAGG 1 cut(s) 971
Bme1390I CCNGG 8 cut(s) 175, 469, 502, 660, 1193, 1425, 1662, 1663
BmeT110I CYCGRG 1 cut(s) 1661
BmgBI CACGTC 1 cut(s) 817
BmgT120I GGNCC 3 cut(s) 183, 901, 1659
BmiI GGNNCC 8 cut(s) 279, 315, 725, 1264, 1650, 1705, 1738, 1814
BmrFI CCNGG 8 cut(s) 175, 469, 502, 660, 1193, 1425, 1662, 1663
BmsI GCATC 3 cut(s) 719, 1033, 1773
BoxI GACNNNNGTC 1 cut(s) 1295
BpiI GAAGAC 1 cut(s) 971
BpmI CTGGAG 1 cut(s) 429
Bpu14I TTCGAA 1 cut(s) 1364
BpuEI CTTGAG 1 cut(s) 1808
BpuMI CCSGG 4 cut(s) 175, 502, 1662, 1663
BsaBI GATNNNNATC 1 cut(s) 300
BsaHI GRCGYC 1 cut(s) 968
BsaJI CCNNGG 7 cut(s) 173, 468, 659, 971, 1019, 1267, 1661
BsaWI WCCGGW 1 cut(s) 541
Bsc4I CCNNNNNNNGG 5 cut(s) 180, 181, 323, 820, 1427
Bse1I ACTGG 4 cut(s) 37, 1299, 1451, 1542
Bse3DI GCAATG 5 cut(s) 111, 513, 1183, 1386, 1602
Bse8I GATNNNNATC 1 cut(s) 300
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 4 cut(s) 469, 660, 1193, 1425
BseDI CCNNGG 7 cut(s) 173, 468, 659, 971, 1019, 1267, 1661
BseGI GGATG 2 cut(s) 669, 1764
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 5 cut(s) 180, 181, 323, 820, 1427
BseMI GCAATG 5 cut(s) 111, 513, 1183, 1386, 1602
BseMII CTCAG 6 cut(s) 92, 419, 707, 1278, 1355, 1530
BseNI ACTGG 4 cut(s) 37, 1299, 1451, 1542
BseRI GAGGAG 1 cut(s) 198
BseSI GKGCMC 1 cut(s) 837
BseX3I CGGCCG 1 cut(s) 1407
BsgI GTGCAG 1 cut(s) 1650
Bsh1236I CGCG 1 cut(s) 821
Bsh1285I CGRYCG 1 cut(s) 1410
BshFI GGCC 4 cut(s) 185, 903, 1409, 1660
BshNI GGYRCC 2 cut(s) 313, 1736
BsiEI CGRYCG 1 cut(s) 1410
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 1661
BsiSI CCGG 4 cut(s) 175, 501, 542, 1662
BslI CCNNNNNNNGG 5 cut(s) 180, 181, 323, 820, 1427
BsmAI GTCTC 4 cut(s) 976, 1068, 1194, 1283
BsmBI CGTCTC 1 cut(s) 1194
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 4 cut(s) 185, 903, 1409, 1660
BsoBI CYCGRG 1 cut(s) 1661
Bsp119I TTCGAA 1 cut(s) 1364
Bsp1286I GDGCHC 3 cut(s) 267, 837, 1653
Bsp13I TCCGGA 1 cut(s) 541
Bsp143I GATC 8 cut(s) 70, 295, 301, 436, 544, 858, 1262, 1812
BspACI CCGC 6 cut(s) 237, 275, 585, 821, 1421, 1657
BspANI GGCC 4 cut(s) 185, 903, 1409, 1660
BspCNI CTCAG 6 cut(s) 91, 420, 708, 1279, 1354, 1529
BspEI TCCGGA 1 cut(s) 541
BspFNI CGCG 1 cut(s) 821
BspHI TCATGA 2 cut(s) 547, 1039
BspLI GGNNCC 8 cut(s) 279, 315, 725, 1264, 1650, 1705, 1738, 1814
BspPI GGATC 7 cut(s) 296, 431, 552, 1257, 1270, 1807, 1820
BspT104I TTCGAA 1 cut(s) 1364
BspT107I GGYRCC 2 cut(s) 313, 1736
BsrDI GCAATG 5 cut(s) 111, 513, 1183, 1386, 1602
BsrI ACTGG 4 cut(s) 37, 1299, 1451, 1542
BssECI CCNNGG 7 cut(s) 173, 468, 659, 971, 1019, 1267, 1661
BssMI GATC 8 cut(s) 70, 295, 301, 436, 544, 858, 1262, 1812
BssNAI GTATAC 1 cut(s) 611
BssNI GRCGYC 1 cut(s) 968
BssT1I CCWWGG 3 cut(s) 971, 1019, 1267
Bst1107I GTATAC 1 cut(s) 611
Bst2UI CCWGG 4 cut(s) 469, 660, 1193, 1425
Bst4CI ACNGT 4 cut(s) 326, 1216, 1384, 1396
Bst6I CTCTTC 1 cut(s) 846
BstACI GRCGYC 1 cut(s) 968
BstAPI GCANNNNNTGC 1 cut(s) 1628
BstBI TTCGAA 1 cut(s) 1364
BstC8I GCNNGC 5 cut(s) 594, 978, 1137, 1329, 1609
BstDEI CTNAG 7 cut(s) 78, 428, 716, 1287, 1341, 1516, 1784
BstEII GGTNACC 1 cut(s) 646
BstF5I GGATG 2 cut(s) 669, 1764
BstFNI CGCG 1 cut(s) 821
BstKTI GATC 8 cut(s) 73, 298, 304, 439, 547, 861, 1265, 1815
BstMAI GTCTC 4 cut(s) 976, 1068, 1194, 1283
BstMBI GATC 8 cut(s) 70, 295, 301, 436, 544, 858, 1262, 1812
BstMCI CGRYCG 1 cut(s) 1410
BstNI CCWGG 4 cut(s) 469, 660, 1193, 1425
BstNSI RCATGY 5 cut(s) 218, 386, 805, 920, 1615
BstPAI GACNNNNGTC 1 cut(s) 1295
BstPI GGTNACC 1 cut(s) 646
BstSCI CCNGG 8 cut(s) 173, 467, 500, 658, 1191, 1423, 1660, 1661
BstSLI GKGCMC 1 cut(s) 837
BstUI CGCG 1 cut(s) 821
BstV2I GAAGAC 1 cut(s) 971
BstX2I RGATCY 2 cut(s) 1262, 1812
BstYI RGATCY 2 cut(s) 1262, 1812
BstZ17I GTATAC 1 cut(s) 611
BstZI CGGCCG 1 cut(s) 1407
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 4 cut(s) 185, 903, 1409, 1660
BtrI CACGTC 1 cut(s) 817
BtsCI GGATG 2 cut(s) 669, 1764
BtsI GCAGTG 2 cut(s) 377, 1650
BtsIMutI CAGTG 5 cut(s) 377, 713, 1292, 1524, 1650
Cac8I GCNNGC 5 cut(s) 594, 978, 1137, 1329, 1609
CciI TCATGA 2 cut(s) 547, 1039
Cfr13I GGNCC 3 cut(s) 183, 901, 1659
Cfr9I CCCGGG 1 cut(s) 1661
CseI GACGC 1 cut(s) 976
Csp6I GTAC 2 cut(s) 955, 1281
CviAII CATG 8 cut(s) 215, 383, 404, 548, 802, 917, 1040, 1612
CviQI GTAC 2 cut(s) 955, 1281
DdeI CTNAG 7 cut(s) 78, 428, 716, 1287, 1341, 1516, 1784
DpnI GATC 8 cut(s) 72, 297, 303, 438, 546, 860, 1264, 1814
DpnII GATC 8 cut(s) 70, 295, 301, 436, 544, 858, 1262, 1812
DraI TTTAAA 2 cut(s) 531, 1435
EaeI YGGCCR 1 cut(s) 1407
EagI CGGCCG 1 cut(s) 1407
Eam1104I CTCTTC 1 cut(s) 846
EarI CTCTTC 1 cut(s) 846
EclXI CGGCCG 1 cut(s) 1407
Eco130I CCWWGG 3 cut(s) 971, 1019, 1267
Eco24I GRGCYC 1 cut(s) 1653
Eco52I CGGCCG 1 cut(s) 1407
Eco88I CYCGRG 1 cut(s) 1661
Eco91I GGTNACC 1 cut(s) 646
EcoO65I GGTNACC 1 cut(s) 646
EcoRI GAATTC 1 cut(s) 122
EcoRII CCWGG 4 cut(s) 467, 658, 1191, 1423
EcoT14I CCWWGG 3 cut(s) 971, 1019, 1267
EcoT38I GRGCYC 1 cut(s) 1653
ErhI CCWWGG 3 cut(s) 971, 1019, 1267
Esp3I CGTCTC 1 cut(s) 1194
FaeI CATG 8 cut(s) 218, 386, 407, 551, 805, 920, 1043, 1615
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FatI CATG 8 cut(s) 214, 382, 403, 547, 801, 916, 1039, 1611
FauI CCCGC 1 cut(s) 1428
FauNDI CATATG 1 cut(s) 826
FblI GTMKAC 2 cut(s) 506, 610
FokI GGATG 2 cut(s) 676, 1751
FriOI GRGCYC 1 cut(s) 1653
FspBI CTAG 3 cut(s) 488, 972, 1028
GsuI CTGGAG 1 cut(s) 429
HaeIII GGCC 4 cut(s) 185, 903, 1409, 1660
HapII CCGG 4 cut(s) 175, 501, 542, 1662
HgaI GACGC 1 cut(s) 976
Hin1I GRCGYC 1 cut(s) 968
Hin1II CATG 8 cut(s) 218, 386, 407, 551, 805, 920, 1043, 1615
HincII GTYRAC 2 cut(s) 1563, 1678
HindII GTYRAC 2 cut(s) 1563, 1678
HindIII AAGCTT 1 cut(s) 330
HpaI GTTAAC 1 cut(s) 1678
HpaII CCGG 4 cut(s) 175, 501, 542, 1662
HphI GGTGA 4 cut(s) 47, 251, 338, 468
Hpy166II GTNNAC 5 cut(s) 507, 611, 693, 1563, 1678
Hpy188I TCNGA 5 cut(s) 81, 350, 863, 1288, 1344
Hpy8I GTNNAC 5 cut(s) 507, 611, 693, 1563, 1678
Hpy99I CGWCG 2 cut(s) 821, 1384
HpyAV CCTTC 5 cut(s) 18, 47, 625, 1357, 1460
HpyCH4III ACNGT 4 cut(s) 326, 1216, 1384, 1396
HpyCH4IV ACGT 6 cut(s) 251, 816, 865, 957, 1203, 1283
HpyF3I CTNAG 7 cut(s) 78, 428, 716, 1287, 1341, 1516, 1784
HpySE526I ACGT 6 cut(s) 251, 816, 865, 957, 1203, 1283
Hsp92I GRCGYC 1 cut(s) 968
Hsp92II CATG 8 cut(s) 218, 386, 407, 551, 805, 920, 1043, 1615
Kpn2I TCCGGA 1 cut(s) 541
KspAI GTTAAC 1 cut(s) 1678
Kzo9I GATC 8 cut(s) 70, 295, 301, 436, 544, 858, 1262, 1812
LmnI GCTCC 2 cut(s) 1469, 1648
LweI GCATC 3 cut(s) 719, 1033, 1773
MaeI CTAG 3 cut(s) 488, 972, 1028
MaeII ACGT 6 cut(s) 251, 816, 865, 957, 1203, 1283
MaeIII GTNAC 7 cut(s) 247, 474, 580, 646, 779, 1210, 1396
MalI GATC 8 cut(s) 72, 297, 303, 438, 546, 860, 1264, 1814
MboI GATC 8 cut(s) 70, 295, 301, 436, 544, 858, 1262, 1812
MboII GAAGA 6 cut(s) 98, 133, 430, 863, 976, 1164
MflI RGATCY 2 cut(s) 1262, 1812
MhlI GDGCHC 3 cut(s) 267, 837, 1653
MlyI GAGTC 4 cut(s) 482, 532, 688, 1594
MnlI CCTC 6 cut(s) 219, 266, 713, 1074, 1421, 1832
MroI TCCGGA 1 cut(s) 541
MroXI GAANNNNTTC 2 cut(s) 57, 1360
MslI CAYNNNNRTG 1 cut(s) 1616
MspA1I CMGCKG 3 cut(s) 26, 68, 119
MspI CCGG 4 cut(s) 175, 501, 542, 1662
MspR9I CCNGG 8 cut(s) 175, 469, 502, 660, 1193, 1425, 1662, 1663
Mva1269I GAATGC 1 cut(s) 357
MvaI CCWGG 4 cut(s) 469, 660, 1193, 1425
MvnI CGCG 1 cut(s) 821
NciI CCSGG 4 cut(s) 175, 502, 1662, 1663
NdeI CATATG 1 cut(s) 826
NdeII GATC 8 cut(s) 70, 295, 301, 436, 544, 858, 1262, 1812
NlaIII CATG 8 cut(s) 218, 386, 407, 551, 805, 920, 1043, 1615
NlaIV GGNNCC 8 cut(s) 279, 315, 725, 1264, 1650, 1705, 1738, 1814
NmeAIII GCCGAG 1 cut(s) 1165
NmuCI GTSAC 2 cut(s) 474, 580
NspI RCATGY 5 cut(s) 218, 386, 805, 920, 1615
NspV TTCGAA 1 cut(s) 1364
PagI TCATGA 2 cut(s) 547, 1039
PciI ACATGT 1 cut(s) 214
PcsI WCGNNNNNNNCGW 1 cut(s) 1704
PctI GAATGC 1 cut(s) 357
PdmI GAANNNNTTC 2 cut(s) 57, 1360
PfeI GAWTC 6 cut(s) 4, 167, 551, 748, 1361, 1538
PflFI GACNNNGTC 2 cut(s) 867, 1205
PleI GAGTC 4 cut(s) 481, 532, 688, 1593
PpsI GAGTC 4 cut(s) 481, 532, 688, 1593
PscI ACATGT 1 cut(s) 214
PshAI GACNNNNGTC 1 cut(s) 1295
PsiI TTATAA 1 cut(s) 989
Psp1406I AACGTT 1 cut(s) 251
Psp6I CCWGG 4 cut(s) 467, 658, 1191, 1423
PspEI GGTNACC 1 cut(s) 646
PspGI CCWGG 4 cut(s) 467, 658, 1191, 1423
PspN4I GGNNCC 8 cut(s) 279, 315, 725, 1264, 1650, 1705, 1738, 1814
PspPI GGNCC 3 cut(s) 183, 901, 1659
PsuI RGATCY 2 cut(s) 1262, 1812
PsyI GACNNNGTC 2 cut(s) 867, 1205
PvuII CAGCTG 3 cut(s) 26, 68, 119
RsaI GTAC 2 cut(s) 956, 1282
RsaNI GTAC 2 cut(s) 955, 1281
RseI CAYNNNNRTG 1 cut(s) 1616
Sau3AI GATC 8 cut(s) 70, 295, 301, 436, 544, 858, 1262, 1812
Sau96I GGNCC 3 cut(s) 183, 901, 1659
SchI GAGTC 4 cut(s) 482, 532, 688, 1594
ScrFI CCNGG 8 cut(s) 175, 469, 502, 660, 1193, 1425, 1662, 1663
SduI GDGCHC 3 cut(s) 267, 837, 1653
SfaNI GCATC 3 cut(s) 719, 1033, 1773
SfuI TTCGAA 1 cut(s) 1364
SmaI CCCGGG 1 cut(s) 1663
SmiMI CAYNNNNRTG 1 cut(s) 1616
SmlI CTYRAG 1 cut(s) 1823
SmoI CTYRAG 1 cut(s) 1823
SrfI GCCCGGGC 1 cut(s) 1663
SsiI CCGC 6 cut(s) 237, 275, 585, 821, 1421, 1657
SspI AATATT 1 cut(s) 103
SspMI CTAG 3 cut(s) 488, 972, 1028
StyD4I CCNGG 8 cut(s) 173, 467, 500, 658, 1191, 1423, 1660, 1661
StyI CCWWGG 3 cut(s) 971, 1019, 1267
TaaI ACNGT 4 cut(s) 326, 1216, 1384, 1396
TaiI ACGT 6 cut(s) 254, 819, 868, 960, 1206, 1286
TaqI TCGA 3 cut(s) 394, 669, 1364
TauI GCSGC 2 cut(s) 824, 1660
TfiI GAWTC 6 cut(s) 4, 167, 551, 748, 1361, 1538
TscAI CASTG 5 cut(s) 384, 720, 1299, 1524, 1650
TseFI GTSAC 2 cut(s) 474, 580
Tsp45I GTSAC 2 cut(s) 474, 580
TspDTI ATGAA 8 cut(s) 17, 33, 444, 564, 696, 1028, 1056, 1551
TspGWI ACGGA 1 cut(s) 1696
TspMI CCCGGG 1 cut(s) 1661
TspRI CASTG 5 cut(s) 384, 720, 1299, 1524, 1650
Tth111I GACNNNGTC 2 cut(s) 867, 1205
XapI RAATTY 5 cut(s) 122, 358, 770, 1109, 1256
XceI RCATGY 5 cut(s) 218, 386, 805, 920, 1615
XmaI CCCGGG 1 cut(s) 1661
XmaJI CCTAGG 1 cut(s) 971
XmiI GTMKAC 2 cut(s) 506, 610
XmnI GAANNNNTTC 2 cut(s) 57, 1360
XspI CTAG 3 cut(s) 488, 972, 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.