Rh2BG221200

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000319
Physical Location & Seq
Forward (+)
21402214 .. 21408042
5829 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG221200.1

Sequence Viewer

Length: 1512 bp
ATGGATCCTTCGCTGTATGAGGCAGCAGCATCAGGTGATCTTCGTTTTCTCAAAGAAGTTGGAGATGGAGATAGATCAGTTGATAGTCTCTTTCAGAAGACCCCTAAAAATAACAATGTTCTTCACATAGCTGCTCAGTTCAAGCAAACACAATTCTTCAAGGAATTCCCAAATCAGATTCAATCTCCATTGTTTTGGGCCACCAACACCAAGGGTGACACTCCCATGCACATTGCTGCTAGGGTAGGTTGTGTTGAACTAGTTGAGTTCCTCATCGATCACGCGAAAAAGTTACGGACTGATGCAGCAGATGAGGAGACCGGACCAGCCGATGCTGAAGCTTATAAAGAGTTACTTCGAATGACTAATTCGGAAAAAGATACAGCTTTGCATGTTGCTGTTAAAAGCGGGTACCATGGTGTTGTCATTCTGTTAATGGATGCTGATCCTGAATTGTGCTGTTATACCAATAACGCCAAAGAATCTCCTTTGTTCTTAGCTGTTAGCAAGGGGTTTCCAGAAATTGGTTCTTGTATTTTGGACAAGTCTTCAGCATCTCTTTCTTTCGAGGGGGTTAAAGGTGTCACAGCTTTGCATGCAGCAGTAACCCGCAATTCAACCTGCAAAGACATCGTGACGATGATGGTGTCCAAAAATCCTGAAATAATCAAAGAAGTTGATGAACTTGGTTGGACTCCCTTGCACTATGCAGCATTCAGAGGGAATGTATCGGCAATTAGATCGCTGATAAAACATAACAGTTCTGTAGCTTACATATTAGACAACTGTGGAATGTCTGCTCTTCATGTTGCAGCGTACGGAGGCCACATAAAAGTAATGAAAGAATTGATTCGATTGCGGCCTGATACTTGTGATCTGCTCAACCACAAAGGCCAAACGGTTGTACATGCTGCAGTTTTGGGTGAGAAGCCGGCTGTTATTAGGTATATTTTGAAGACACCTAAGCTAGCAGGACTTGTAAACGAAGCAGACGATGATGGAAATACTCCTTTGCATCTGGCTGCCTCTATACCGAATTCTGCAATTATGAAAGCCTTGGCAAGGGATTGCAAAGTTGACAAGACTGCAACCAATGATCATCACTCGAAAGCGGTTGATATATATCTTGGTAACAATATTGAACTGTACACCATTGGTAGACGAAGCGTCTTGCATAATTTGGGGAGGTCCATCGGTGTGCCAATTTTCCAGCGATATGTGCGCCGTGATTTCATGAAATTGGAATCATCGGAGGAGAAGAATGACTTCATGAAATTAGAATCATCGGAGGAGAAGAAGGCTACATCCACCACAGTGACCATTGCAAACGAAAAATCAGTCAAAGCTTCGCTGGATGACTCGAAAAGATTTGACACAAATCTACTAGTTGCGGTGCTTATTGCTACCGTCAGTTTTTCAGCCGCTTTCACCATGCCTGGGGGATTTAATAGCGACGGCATCAATGGTGTTGGCATTTGCCTCGGGTATGTTTGTAGTGCTACCCAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

503

Amino Acids

54.84

Weight (kDa)

6.47

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 6 - 97 1e-07 Ankyrin repeats (3 copies)
Ank_4 PF13637 198 - 249 2.7e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 210 - 285 3.6e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 241 - 281 1.4e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 291 - 363 4.3e-07 Ankyrin repeats (3 copies)
PGG PF13962 456 - 486 1e-08 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 629
Acc65I GGTACC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 411
AccB7I CCANNNNNTGG 1 cut(s) 524
AccI GTMKAC 1 cut(s) 1159
AccII CGCG 1 cut(s) 284
AciI CCGC 6 cut(s) 408, 610, 859, 1112, 1391, 1422
AclWI GGATC 2 cut(s) 12, 440
AcsI RAATTY 2 cut(s) 164, 1036
AcuI CTGAAG 2 cut(s) 357, 534
AfaI GTAC 4 cut(s) 413, 818, 906, 1148
AfiI CCNNNNNNNGG 3 cut(s) 524, 1062, 1437
AgsI TTSAA 7 cut(s) 142, 160, 182, 257, 618, 955, 1142
AhdI GACNNNNNGTC 1 cut(s) 1166
AhlI ACTAGT 2 cut(s) 259, 1384
AjnI CCWGG 1 cut(s) 1435
AleI CACNNNNGTG 1 cut(s) 1313
AluBI AGCT 8 cut(s) 131, 341, 386, 500, 590, 770, 967, 1346
AluI AGCT 8 cut(s) 131, 341, 386, 500, 590, 770, 967, 1346
Alw26I GTCTC 2 cut(s) 92, 311
AlwI GGATC 2 cut(s) 12, 440
Ama87I CYCGRG 1 cut(s) 1481
AoxI GGCC 4 cut(s) 198, 823, 860, 892
ApoI RAATTY 2 cut(s) 164, 1036
ArsI GACNNNNNNTTYG 2 cut(s) 1323, 1355
Asp700I GAANNNNTTC 2 cut(s) 849, 1265
Asp718I GGTACC 1 cut(s) 411
AspLEI GCGC 1 cut(s) 1224
AspS9I GGNCC 3 cut(s) 198, 323, 1188
AsuHPI GGTGA 4 cut(s) 47, 227, 935, 1420
AsuII TTCGAA 1 cut(s) 358
AsuNHI GCTAGC 1 cut(s) 967
AvaI CYCGRG 1 cut(s) 1481
AvaII GGWCC 2 cut(s) 323, 1188
BaeI ACNNNNGTAYC 2 cut(s) 403, 436
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 411
BbsI GAAGAC 3 cut(s) 104, 540, 962
BccI CCATC 4 cut(s) 59, 637, 992, 1199
BceAI ACGGC 2 cut(s) 1209, 1471
BcgI CGANNNNNNTGC 6 cut(s) 613, 647, 723, 757, 1462, 1496
BciT130I CCWGG 1 cut(s) 1437
BclI TGATCA 1 cut(s) 1096
BcoDI GTCTC 2 cut(s) 92, 311
BcuI ACTAGT 2 cut(s) 259, 1384
BfaI CTAG 4 cut(s) 240, 260, 968, 1385
BfmI CTRYAG 2 cut(s) 765, 912
BfuAI ACCTGC 1 cut(s) 629
Bme1390I CCNGG 1 cut(s) 1437
Bme18I GGWCC 2 cut(s) 323, 1188
BmeRI GACNNNNNGTC 1 cut(s) 1166
BmeT110I CYCGRG 1 cut(s) 1481
BmgT120I GGNCC 3 cut(s) 198, 323, 1188
BmiI GGNNCC 2 cut(s) 6, 413
BmrFI CCNGG 1 cut(s) 1437
BmsI GCATC 7 cut(s) 38, 292, 322, 430, 563, 1024, 1467
BmtI GCTAGC 1 cut(s) 971
BpiI GAAGAC 3 cut(s) 104, 540, 962
Bpu10I CCTNAGC 1 cut(s) 963
Bpu14I TTCGAA 1 cut(s) 358
Bsa29I ATCGAT 1 cut(s) 276
BsaBI GATNNNNATC 2 cut(s) 444, 1122
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 5 cut(s) 210, 415, 1056, 1436, 1480
BsaWI WCCGGW 1 cut(s) 320
Bsc4I CCNNNNNNNGG 3 cut(s) 524, 1062, 1437
Bse118I RCCGGY 1 cut(s) 931
Bse3DI GCAATG 2 cut(s) 231, 1320
Bse8I GATNNNNATC 2 cut(s) 444, 1122
BseBI CCWGG 1 cut(s) 1437
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 5 cut(s) 210, 415, 1056, 1436, 1480
BseGI GGATG 3 cut(s) 445, 1304, 1360
BseJI GATNNNNATC 2 cut(s) 444, 1122
BseLI CCNNNNNNNGG 3 cut(s) 524, 1062, 1437
BseMI GCAATG 2 cut(s) 231, 1320
BseMII CTCAG 1 cut(s) 149
BseRI GAGGAG 3 cut(s) 329, 1268, 1304
Bsh1236I CGCG 1 cut(s) 284
BshFI GGCC 4 cut(s) 200, 825, 862, 894
BshNI GGYRCC 1 cut(s) 411
BshVI ATCGAT 1 cut(s) 276
BsiHKCI CYCGRG 1 cut(s) 1481
BsiSI CCGG 2 cut(s) 321, 932
BsiWI CGTACG 1 cut(s) 816
BslI CCNNNNNNNGG 3 cut(s) 524, 1062, 1437
BsmAI GTCTC 2 cut(s) 92, 311
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 4 cut(s) 200, 825, 862, 894
Bso31I GGTCTC 1 cut(s) 311
BsoBI CYCGRG 1 cut(s) 1481
Bsp119I TTCGAA 1 cut(s) 358
Bsp1407I TGTACA 2 cut(s) 904, 1146
Bsp143I GATC 8 cut(s) 4, 37, 74, 277, 445, 740, 874, 1096
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 6 cut(s) 408, 610, 859, 1112, 1391, 1422
BspANI GGCC 4 cut(s) 200, 825, 862, 894
BspCNI CTCAG 1 cut(s) 148
BspDI ATCGAT 1 cut(s) 276
BspFNI CGCG 1 cut(s) 284
BspHI TCATGA 2 cut(s) 1233, 1269
BspLI GGNNCC 2 cut(s) 6, 413
BspMAI CTGCAG 1 cut(s) 916
BspMI ACCTGC 1 cut(s) 629
BspOI GCTAGC 1 cut(s) 971
BspPI GGATC 2 cut(s) 12, 440
BspQI GCTCTTC 1 cut(s) 807
BspT104I TTCGAA 1 cut(s) 358
BspT107I GGYRCC 1 cut(s) 411
BspTNI GGTCTC 1 cut(s) 311
BsrDI GCAATG 2 cut(s) 231, 1320
BsrFI RCCGGY 1 cut(s) 931
BsrGI TGTACA 2 cut(s) 904, 1146
BssAI RCCGGY 1 cut(s) 931
BssECI CCNNGG 5 cut(s) 210, 415, 1056, 1436, 1480
BssMI GATC 8 cut(s) 4, 37, 74, 277, 445, 740, 874, 1096
BssT1I CCWWGG 3 cut(s) 210, 415, 1056
Bst2UI CCWGG 1 cut(s) 1437
Bst4CI ACNGT 6 cut(s) 761, 788, 901, 1146, 1315, 1408
Bst6I CTCTTC 1 cut(s) 807
BstAUI TGTACA 2 cut(s) 904, 1146
BstBI TTCGAA 1 cut(s) 358
BstC8I GCNNGC 3 cut(s) 597, 933, 969
BstDEI CTNAG 3 cut(s) 135, 496, 963
BstDSI CCRYGG 1 cut(s) 415
BstENI CCTNNNNNAGG 1 cut(s) 1060
BstF5I GGATG 3 cut(s) 445, 1304, 1360
BstFNI CGCG 1 cut(s) 284
BstHHI GCGC 1 cut(s) 1224
BstKTI GATC 8 cut(s) 7, 40, 77, 280, 448, 743, 877, 1099
BstMAI GTCTC 2 cut(s) 92, 311
BstMBI GATC 8 cut(s) 4, 37, 74, 277, 445, 740, 874, 1096
BstMWI GCNNNNNNNGC 2 cut(s) 596, 1219
BstNI CCWGG 1 cut(s) 1437
BstNSI RCATGY 3 cut(s) 395, 599, 911
BstSCI CCNGG 1 cut(s) 1435
BstSFI CTRYAG 2 cut(s) 765, 912
BstUI CGCG 1 cut(s) 284
BstV2I GAAGAC 3 cut(s) 104, 540, 962
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 195
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 1 cut(s) 276
BsuRI GGCC 4 cut(s) 200, 825, 862, 894
BsuTUI ATCGAT 1 cut(s) 276
BtgI CCRYGG 1 cut(s) 415
BtsCI GGATG 3 cut(s) 445, 1304, 1360
BtsIMutI CAGTG 1 cut(s) 1320
BveI ACCTGC 1 cut(s) 629
Cac8I GCNNGC 3 cut(s) 597, 933, 969
CciI TCATGA 2 cut(s) 1233, 1269
CfoI GCGC 1 cut(s) 1224
Cfr10I RCCGGY 1 cut(s) 931
Cfr13I GGNCC 3 cut(s) 198, 323, 1188
ClaI ATCGAT 1 cut(s) 276
CseI GACGC 1 cut(s) 1156
Csp6I GTAC 4 cut(s) 412, 817, 905, 1147
CviAII CATG 9 cut(s) 226, 392, 416, 596, 806, 908, 1234, 1270, 1432
CviQI GTAC 4 cut(s) 412, 817, 905, 1147
DdeI CTNAG 3 cut(s) 135, 496, 963
DpnI GATC 8 cut(s) 6, 39, 76, 279, 447, 742, 876, 1098
DpnII GATC 8 cut(s) 4, 37, 74, 277, 445, 740, 874, 1096
DriI GACNNNNNGTC 1 cut(s) 1166
Eam1104I CTCTTC 1 cut(s) 807
Eam1105I GACNNNNNGTC 1 cut(s) 1166
EarI CTCTTC 1 cut(s) 807
Eco130I CCWWGG 3 cut(s) 210, 415, 1056
Eco31I GGTCTC 1 cut(s) 311
Eco47I GGWCC 2 cut(s) 323, 1188
Eco57I CTGAAG 2 cut(s) 357, 534
Eco88I CYCGRG 1 cut(s) 1481
EcoNI CCTNNNNNAGG 1 cut(s) 1060
EcoRI GAATTC 2 cut(s) 164, 1036
EcoRII CCWGG 1 cut(s) 1435
EcoT14I CCWWGG 3 cut(s) 210, 415, 1056
ErhI CCWWGG 3 cut(s) 210, 415, 1056
FaeI CATG 9 cut(s) 229, 395, 419, 599, 809, 911, 1237, 1273, 1435
FalI AAGNNNNNCTT 4 cut(s) 339, 371, 1250, 1282
FatI CATG 9 cut(s) 225, 391, 415, 595, 805, 907, 1233, 1269, 1431
FauI CCCGC 2 cut(s) 401, 617
FbaI TGATCA 1 cut(s) 1096
FblI GTMKAC 1 cut(s) 1159
FokI GGATG 3 cut(s) 452, 1291, 1367
FspBI CTAG 4 cut(s) 240, 260, 968, 1385
GlaI GCGC 1 cut(s) 1223
HaeIII GGCC 4 cut(s) 200, 825, 862, 894
HapII CCGG 2 cut(s) 321, 932
HgaI GACGC 1 cut(s) 1156
HhaI GCGC 1 cut(s) 1224
Hin1II CATG 9 cut(s) 229, 395, 419, 599, 809, 911, 1237, 1273, 1435
Hin6I GCGC 1 cut(s) 1222
HinP1I GCGC 1 cut(s) 1222
HincII GTYRAC 1 cut(s) 1078
HindII GTYRAC 1 cut(s) 1078
HindIII AAGCTT 2 cut(s) 339, 1344
HinfI GANTC 7 cut(s) 178, 482, 694, 850, 1244, 1280, 1358
HpaII CCGG 2 cut(s) 321, 932
HphI GGTGA 4 cut(s) 47, 227, 935, 1420
Hpy166II GTNNAC 4 cut(s) 982, 1078, 1149, 1160
Hpy188I TCNGA 6 cut(s) 96, 177, 373, 719, 1252, 1288
Hpy188III TCNNGA 6 cut(s) 449, 518, 634, 659, 1234, 1270
Hpy8I GTNNAC 4 cut(s) 982, 1078, 1149, 1160
Hpy99I CGWCG 1 cut(s) 1457
HpyAV CCTTC 2 cut(s) 18, 1291
HpyCH4III ACNGT 6 cut(s) 761, 788, 901, 1146, 1315, 1408
HpyF10VI GCNNNNNNNGC 2 cut(s) 596, 1219
HpyF3I CTNAG 3 cut(s) 135, 496, 963
Hsp92II CATG 9 cut(s) 229, 395, 419, 599, 809, 911, 1237, 1273, 1435
HspAI GCGC 1 cut(s) 1222
KpnI GGTACC 1 cut(s) 415
KroI GCCGGC 1 cut(s) 931
KroNI GCCGGC 1 cut(s) 933
Ksp22I TGATCA 1 cut(s) 1096
Kzo9I GATC 8 cut(s) 4, 37, 74, 277, 445, 740, 874, 1096
LguI GCTCTTC 1 cut(s) 807
LweI GCATC 7 cut(s) 38, 292, 322, 430, 563, 1024, 1467
MaeI CTAG 4 cut(s) 240, 260, 968, 1385
MaeIII GTNAC 8 cut(s) 215, 291, 351, 583, 604, 634, 1130, 1315
MalI GATC 8 cut(s) 6, 39, 76, 279, 447, 742, 876, 1098
MboI GATC 8 cut(s) 4, 37, 74, 277, 445, 740, 874, 1096
MboII GAAGA 9 cut(s) 32, 109, 113, 148, 540, 794, 967, 1270, 1306
MflI RGATCY 1 cut(s) 4
MlyI GAGTC 2 cut(s) 688, 1352
MmeI TCCRAC 2 cut(s) 40, 671
MroNI GCCGGC 1 cut(s) 931
MroXI GAANNNNTTC 2 cut(s) 849, 1265
MseI TTAA 4 cut(s) 402, 434, 576, 1446
MslI CAYNNNNRTG 3 cut(s) 224, 1196, 1313
MspI CCGG 2 cut(s) 321, 932
MspR9I CCNGG 1 cut(s) 1437
Mva1269I GAATGC 1 cut(s) 713
MvaI CCWGG 1 cut(s) 1437
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 2 cut(s) 596, 1219
NaeI GCCGGC 1 cut(s) 933
NcoI CCATGG 1 cut(s) 415
NdeII GATC 8 cut(s) 4, 37, 74, 277, 445, 740, 874, 1096
NgoMIV GCCGGC 1 cut(s) 931
NheI GCTAGC 1 cut(s) 967
NlaIII CATG 9 cut(s) 229, 395, 419, 599, 809, 911, 1237, 1273, 1435
NlaIV GGNNCC 2 cut(s) 6, 413
NmuCI GTSAC 4 cut(s) 215, 583, 634, 1315
NspI RCATGY 3 cut(s) 395, 599, 911
NspV TTCGAA 1 cut(s) 358
OliI CACNNNNGTG 1 cut(s) 1313
PaeI GCATGC 1 cut(s) 599
PagI TCATGA 2 cut(s) 1233, 1269
PciSI GCTCTTC 1 cut(s) 807
PcsI WCGNNNNNNNCGW 1 cut(s) 990
PctI GAATGC 1 cut(s) 713
PdiI GCCGGC 1 cut(s) 933
PdmI GAANNNNTTC 2 cut(s) 849, 1265
PfeI GAWTC 5 cut(s) 178, 482, 850, 1244, 1280
Pfl23II CGTACG 1 cut(s) 816
PflMI CCANNNNNTGG 1 cut(s) 524
PleI GAGTC 2 cut(s) 688, 1352
PpsI GAGTC 2 cut(s) 688, 1352
PsiI TTATAA 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 1435
PspGI CCWGG 1 cut(s) 1435
PspLI CGTACG 1 cut(s) 816
PspN4I GGNNCC 2 cut(s) 6, 413
PspPI GGNCC 3 cut(s) 198, 323, 1188
PstI CTGCAG 1 cut(s) 916
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 4 cut(s) 413, 818, 906, 1148
RsaNI GTAC 4 cut(s) 412, 817, 905, 1147
RseI CAYNNNNRTG 3 cut(s) 224, 1196, 1313
SapI GCTCTTC 1 cut(s) 807
SaqAI TTAA 4 cut(s) 402, 434, 576, 1446
Sau3AI GATC 8 cut(s) 4, 37, 74, 277, 445, 740, 874, 1096
Sau96I GGNCC 3 cut(s) 198, 323, 1188
SchI GAGTC 2 cut(s) 688, 1352
ScrFI CCNGG 1 cut(s) 1437
SfaNI GCATC 7 cut(s) 38, 292, 322, 430, 563, 1024, 1467
SfcI CTRYAG 2 cut(s) 765, 912
SfuI TTCGAA 1 cut(s) 358
SinI GGWCC 2 cut(s) 323, 1188
SmiMI CAYNNNNRTG 3 cut(s) 224, 1196, 1313
SpeI ACTAGT 2 cut(s) 259, 1384
SphI GCATGC 1 cut(s) 599
SsiI CCGC 6 cut(s) 408, 610, 859, 1112, 1391, 1422
SspI AATATT 1 cut(s) 1138
SspMI CTAG 4 cut(s) 240, 260, 968, 1385
StyD4I CCNGG 1 cut(s) 1435
StyI CCWWGG 3 cut(s) 210, 415, 1056
TaaI ACNGT 6 cut(s) 761, 788, 901, 1146, 1315, 1408
TaqI TCGA 6 cut(s) 276, 358, 567, 853, 1106, 1361
TatI WGTACW 2 cut(s) 904, 1146
TauI GCSGC 2 cut(s) 862, 1424
TfiI GAWTC 5 cut(s) 178, 482, 850, 1244, 1280
Tru1I TTAA 4 cut(s) 402, 434, 576, 1446
Tru9I TTAA 4 cut(s) 402, 434, 576, 1446
TscAI CASTG 1 cut(s) 1320
TseFI GTSAC 4 cut(s) 215, 583, 634, 1315
Tsp45I GTSAC 4 cut(s) 215, 583, 634, 1315
TspDTI ATGAA 8 cut(s) 696, 794, 854, 1064, 1222, 1250, 1258, 1286
TspGWI ACGGA 2 cut(s) 310, 834
TspRI CASTG 1 cut(s) 1320
Van91I CCANNNNNTGG 1 cut(s) 524
VpaK11BI GGWCC 2 cut(s) 323, 1188
XagI CCTNNNNNAGG 1 cut(s) 1060
XapI RAATTY 2 cut(s) 164, 1036
XceI RCATGY 3 cut(s) 395, 599, 911
XmiI GTMKAC 1 cut(s) 1159
XmnI GAANNNNTTC 2 cut(s) 849, 1265
XspI CTAG 4 cut(s) 240, 260, 968, 1385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.