Rroxscaffold_2G00134330

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000319
Physical Location & Seq
Reverse (-)
71821121 .. 71824449
3329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00134330.1

Sequence Viewer

Length: 786 bp
ATGAAGTGCAACCTTAGGCCATCTCCAACCCACAAGGCTAAAAATAGCCCCGATTTCTTTACTATTCATCAATGTGACATCAACCGTTTCCAACCCATCAAGGATGAAATTTTGAGGCCTCAAGCCCTCAAGGAGCGTCTTCAGTCCCACCGTCCAGAAGAATGCAGTGCAGTAGGGTTTCCAGAAATTGGTTCTTGTATTTTGGACAGGTGTCCATCTTCTCTTTCTTTCGAGGGGGTTAAAGGTGTCACAGCTTTGCGTGCAGCAGTAACCCGCAATTCAACCTGCAAAGATATTGTGGGGATCATGGTGGCCAAAAATCCTGAAATGATCAAAGAAGTTGATGAACTTGGTTGGACTCCCTTACATTATGCAACATTCAGAGGGAATGTTGAAGCAATTAGAACGCTGATAAAATGCGACAGTTCTGTGGCTTACATCTTGGACAAATGTGGAATGTTTGCTCTTCATATTGCGGCTCACGCAGGCCGTATAAATGTAATGAAAGAGTTAATTCGATTGCGGCCTGATACTTGTGATCTGCTTAACCACAAACGCCAAACGGCCCTACATGCTGCAGTTTTGGGTGAGAAGCGGTTTGTCATCACGTATATTTTGAAGACGCCTGAGCTAGCAGGACTTGTAAACGAAGCAGACAATGATGGAAATACTCCTTTGCATCTGGCTGCCTTTAAAAAGAATACTGGAATTATGAGAACCTTGGCAAGGGATTGCAAAGTTGACACGACTGCAACCAATGATATATCATCACTCAAAAGCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

261

Amino Acids

29.02

Weight (kDa)

9.0

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 80 - 141 1.4e-07 Ankyrin repeats (3 copies)
Ank_4 PF13637 94 - 138 4.2e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 156 - 249 2.3e-12 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 293
AccB7I CCANNNNNTGG 1 cut(s) 188
AciI CCGC 4 cut(s) 274, 476, 523, 595
AclWI GGATC 1 cut(s) 311
AcoI YGGCCR 1 cut(s) 312
AcsI RAATTY 1 cut(s) 108
AcuI CTGAAG 1 cut(s) 125
AcyI GRCGYC 1 cut(s) 623
AfiI CCNNNNNNNGG 2 cut(s) 188, 726
AgsI TTSAA 3 cut(s) 282, 395, 619
AluBI AGCT 2 cut(s) 254, 631
AluI AGCT 2 cut(s) 254, 631
AlwI GGATC 1 cut(s) 311
AoxI GGCC 6 cut(s) 17, 116, 312, 487, 524, 564
ApeKI GCWGC 3 cut(s) 263, 575, 686
ApoI RAATTY 1 cut(s) 108
AspS9I GGNCC 1 cut(s) 565
AsuHPI GGTGA 1 cut(s) 599
AsuNHI GCTAGC 1 cut(s) 631
AxyI CCTNAGG 1 cut(s) 14
BalI TGGCCA 1 cut(s) 314
BbsI GAAGAC 2 cut(s) 131, 626
BbvI GCAGC 3 cut(s) 275, 562, 673
BccI CCATC 4 cut(s) 28, 104, 223, 656
BceAI ACGGC 3 cut(s) 474, 579, 765
BclI TGATCA 1 cut(s) 330
BfaI CTAG 1 cut(s) 632
BfmI CTRYAG 1 cut(s) 576
BfuAI ACCTGC 1 cut(s) 293
BisI GCNGC 5 cut(s) 264, 477, 524, 576, 687
BlsI GCNGC 5 cut(s) 265, 478, 525, 577, 688
BmgT120I GGNCC 1 cut(s) 565
BmsI GCATC 1 cut(s) 688
BmtI GCTAGC 1 cut(s) 635
BoxI GACNNNNGTC 1 cut(s) 210
BpiI GAAGAC 2 cut(s) 131, 626
Bpu10I CCTNAGC 1 cut(s) 627
BpuEI CTTGAG 2 cut(s) 105, 113
BsaAI YACGTR 1 cut(s) 609
BsaHI GRCGYC 1 cut(s) 623
BsaJI CCNNGG 1 cut(s) 720
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 726
Bse1I ACTGG 1 cut(s) 709
Bse21I CCTNAGG 1 cut(s) 14
BseDI CCNNGG 1 cut(s) 720
BseGI GGATG 1 cut(s) 109
BseLI CCNNNNNNNGG 2 cut(s) 188, 726
BseMII CTCAG 1 cut(s) 618
BseNI ACTGG 1 cut(s) 709
BseXI GCAGC 3 cut(s) 275, 562, 673
BsgI GTGCAG 2 cut(s) 189, 282
BshFI GGCC 6 cut(s) 19, 118, 314, 489, 526, 566
BslFI GGGAC 1 cut(s) 130
BslI CCNNNNNNNGG 2 cut(s) 188, 726
BsmFI GGGAC 1 cut(s) 130
BsmI GAATGC 1 cut(s) 167
BsnI GGCC 6 cut(s) 19, 118, 314, 489, 526, 566
Bsp143I GATC 3 cut(s) 303, 330, 538
BspACI CCGC 4 cut(s) 274, 476, 523, 595
BspANI GGCC 6 cut(s) 19, 118, 314, 489, 526, 566
BspCNI CTCAG 1 cut(s) 619
BspMAI CTGCAG 1 cut(s) 580
BspMI ACCTGC 1 cut(s) 293
BspOI GCTAGC 1 cut(s) 635
BspPI GGATC 1 cut(s) 311
BspQI GCTCTTC 1 cut(s) 471
BsrI ACTGG 1 cut(s) 709
BssECI CCNNGG 1 cut(s) 720
BssMI GATC 3 cut(s) 303, 330, 538
BssNI GRCGYC 1 cut(s) 623
BssT1I CCWWGG 1 cut(s) 720
Bst4CI ACNGT 3 cut(s) 86, 152, 425
Bst6I CTCTTC 1 cut(s) 471
BstACI GRCGYC 1 cut(s) 623
BstBAI YACGTR 1 cut(s) 609
BstC8I GCNNGC 3 cut(s) 261, 487, 633
BstDEI CTNAG 2 cut(s) 14, 627
BstENI CCTNNNNNAGG 1 cut(s) 724
BstF5I GGATG 1 cut(s) 109
BstKTI GATC 3 cut(s) 306, 333, 541
BstMBI GATC 3 cut(s) 303, 330, 538
BstMWI GCNNNNNNNGC 3 cut(s) 260, 482, 572
BstNSI RCATGY 1 cut(s) 575
BstPAI GACNNNNGTC 1 cut(s) 210
BstSFI CTRYAG 1 cut(s) 576
BstV1I GCAGC 3 cut(s) 275, 562, 673
BstV2I GAAGAC 2 cut(s) 131, 626
Bsu36I CCTNAGG 1 cut(s) 14
BsuRI GGCC 6 cut(s) 19, 118, 314, 489, 526, 566
BtsCI GGATG 1 cut(s) 109
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 1 cut(s) 172
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 3 cut(s) 261, 487, 633
Cfr13I GGNCC 1 cut(s) 565
CseI GACGC 2 cut(s) 125, 631
CviAII CATG 2 cut(s) 307, 572
DdeI CTNAG 2 cut(s) 14, 627
DpnI GATC 3 cut(s) 305, 332, 540
DpnII GATC 3 cut(s) 303, 330, 538
DraI TTTAAA 1 cut(s) 694
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 720
Eco147I AGGCCT 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 125
Eco81I CCTNAGG 1 cut(s) 14
EcoNI CCTNNNNNAGG 1 cut(s) 724
EcoT14I CCWWGG 1 cut(s) 720
ErhI CCWWGG 1 cut(s) 720
FaeI CATG 2 cut(s) 310, 575
FaiI YATR 8 cut(s) 308, 372, 471, 494, 573, 612, 713, 764
FaqI GGGAC 1 cut(s) 130
FatI CATG 2 cut(s) 306, 571
FauI CCCGC 1 cut(s) 281
FbaI TGATCA 1 cut(s) 330
Fnu4HI GCNGC 5 cut(s) 264, 477, 524, 576, 687
FokI GGATG 1 cut(s) 116
Fsp4HI GCNGC 5 cut(s) 264, 477, 524, 576, 687
FspBI CTAG 1 cut(s) 632
GluI GCNGC 5 cut(s) 264, 477, 524, 576, 687
HaeIII GGCC 6 cut(s) 19, 118, 314, 489, 526, 566
HgaI GACGC 2 cut(s) 125, 631
Hin1I GRCGYC 1 cut(s) 623
Hin1II CATG 2 cut(s) 310, 575
HincII GTYRAC 1 cut(s) 742
HindII GTYRAC 1 cut(s) 742
HinfI GANTC 1 cut(s) 358
HphI GGTGA 1 cut(s) 599
Hpy166II GTNNAC 2 cut(s) 646, 742
Hpy188I TCNGA 1 cut(s) 383
Hpy188III TCNNGA 3 cut(s) 155, 182, 323
Hpy8I GTNNAC 2 cut(s) 646, 742
HpyCH4III ACNGT 3 cut(s) 86, 152, 425
HpyCH4IV ACGT 1 cut(s) 608
HpyF10VI GCNNNNNNNGC 3 cut(s) 260, 482, 572
HpyF3I CTNAG 2 cut(s) 14, 627
HpySE526I ACGT 1 cut(s) 608
Hsp92I GRCGYC 1 cut(s) 623
Hsp92II CATG 2 cut(s) 310, 575
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 3 cut(s) 303, 330, 538
LguI GCTCTTC 1 cut(s) 471
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 3 cut(s) 275, 562, 673
LweI GCATC 1 cut(s) 688
MaeI CTAG 1 cut(s) 632
MaeII ACGT 1 cut(s) 608
MaeIII GTNAC 3 cut(s) 74, 247, 268
MalI GATC 3 cut(s) 305, 332, 540
MboI GATC 3 cut(s) 303, 330, 538
MboII GAAGA 5 cut(s) 131, 170, 210, 458, 631
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 6 cut(s) 108, 186, 277, 399, 513, 708
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 1 cut(s) 352
MmeI TCCRAC 3 cut(s) 50, 115, 335
MnlI CCTC 5 cut(s) 108, 129, 137, 226, 377
Mox20I TGGCCA 1 cut(s) 314
MscI TGGCCA 1 cut(s) 314
MseI TTAA 4 cut(s) 240, 512, 546, 693
MslI CAYNNNNRTG 1 cut(s) 72
Msp20I TGGCCA 1 cut(s) 314
Mva1269I GAATGC 1 cut(s) 167
MwoI GCNNNNNNNGC 3 cut(s) 260, 482, 572
NdeII GATC 3 cut(s) 303, 330, 538
NheI GCTAGC 1 cut(s) 631
NlaIII CATG 2 cut(s) 310, 575
NmuCI GTSAC 2 cut(s) 74, 247
NspI RCATGY 1 cut(s) 575
PceI AGGCCT 1 cut(s) 118
PciSI GCTCTTC 1 cut(s) 471
PctI GAATGC 1 cut(s) 167
PflMI CCANNNNNTGG 1 cut(s) 188
PkrI GCNGC 5 cut(s) 265, 478, 525, 577, 688
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
Ppu21I YACGTR 1 cut(s) 609
PshAI GACNNNNGTC 1 cut(s) 210
PspPI GGNCC 1 cut(s) 565
PstI CTGCAG 1 cut(s) 580
RseI CAYNNNNRTG 1 cut(s) 72
SapI GCTCTTC 1 cut(s) 471
SaqAI TTAA 4 cut(s) 240, 512, 546, 693
SatI GCNGC 5 cut(s) 264, 477, 524, 576, 687
Sau3AI GATC 3 cut(s) 303, 330, 538
Sau96I GGNCC 1 cut(s) 565
SchI GAGTC 1 cut(s) 352
SetI ASST 8 cut(s) 15, 212, 247, 256, 287, 611, 633, 722
SfaNI GCATC 1 cut(s) 688
SfcI CTRYAG 1 cut(s) 576
SmiMI CAYNNNNRTG 1 cut(s) 72
SmlI CTYRAG 2 cut(s) 120, 128
SmoI CTYRAG 2 cut(s) 120, 128
Sse9I AATT 6 cut(s) 108, 186, 277, 399, 513, 708
SseBI AGGCCT 1 cut(s) 118
SsiI CCGC 4 cut(s) 274, 476, 523, 595
SspMI CTAG 1 cut(s) 632
StuI AGGCCT 1 cut(s) 118
StyI CCWWGG 1 cut(s) 720
TaaI ACNGT 3 cut(s) 86, 152, 425
TaiI ACGT 1 cut(s) 611
TaqI TCGA 2 cut(s) 231, 517
TasI AATT 6 cut(s) 108, 186, 277, 399, 513, 708
TauI GCSGC 2 cut(s) 479, 526
Tru1I TTAA 4 cut(s) 240, 512, 546, 693
Tru9I TTAA 4 cut(s) 240, 512, 546, 693
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 2 cut(s) 74, 247
TseI GCWGC 3 cut(s) 263, 575, 686
Tsp45I GTSAC 2 cut(s) 74, 247
TspDTI ATGAA 6 cut(s) 17, 56, 120, 360, 458, 518
TspRI CASTG 1 cut(s) 172
Van91I CCANNNNNTGG 1 cut(s) 188
XagI CCTNNNNNAGG 1 cut(s) 724
XapI RAATTY 1 cut(s) 108
XceI RCATGY 1 cut(s) 575
XspI CTAG 1 cut(s) 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.