Rh2BG221800

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000319
Physical Location & Seq
Forward (+)
21446263 .. 21452572
6310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG221800.1

Sequence Viewer

Length: 849 bp
ATGGAGTTCAGTGCCATATGCATGGATCCTTTGCTGTATGAAGCGGCTGCATGCGGTGATATTCAATTTCTCAAAGAAGTTAGAGATGGGGAGAGATTAGTTGATATATTTCAGAAGACCCCTAAAGCCAACAACGTTCTTCACATAGCTGCTCAGTTCAAGCAAACACAGTTCTTCAAGGAATTCCCAAATCAGATTCAATCTCCATTGTTTTGGGCCACTAACACCAAAGGTGACACACCCATGCACATTGCTGCTAGGGTAGGTTGTGTCAAACTAGTTGAGTTCCTCATCGATCACGCAAGAAAGTTGCAGACTGATGCAGCAGATGAGGAGACCGGACCATCCGATGCTGAAGCTTATAAAGAGTTACTTCGAATGACTAATTCGGAAAAAGATACAGCTTTGCATGTTGCTGTTAAATGCGGGCACCTTGATGTTGTGATTTTGTTAATGGATGATCCAGAATTGTGTTGTTATACCAATAATGCCAATGAATCTCCATTGTTCTTAGCTATCTGCAAGGGGTTTCCAAAAATTGGTTCTTGTATTTTGGACAAGTCTCCAGCATCTCTTTCTTTCGAGGGGGTTAAAGGTATCACAGCTTTGCATGCAGCAGTAACCCGCAATTCAACCTGCAAAGATATTGTGGAGATTATGGTGTCCAAAAATCCTGAATTGATCAAAGAAGTTGATGAACTTGGTTGGACCCCCTTACACTATGCAGCATTCAGAGGGAATGTTGAAGCAATTAGAACGCTGATAAAATGCGATAGTTCTGTGGTTTACATCTTGGACAAATGTGGAATGTCTGCTCTTCATGTTGCGGCCCGCGCCCTACAAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

282

Amino Acids

31.12

Weight (kDa)

5.52

Isoelectric Point (pI)

31.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 12 - 102 1e-07 Ankyrin repeats (3 copies)
Ank_5 PF13857 125 - 169 8.3e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 203 - 278 9.9e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 203 - 255 6.3e-08 Ankyrin repeats (many copies)
Ank PF00023 235 - 259 8.2e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 363
Acc36I ACCTGC 1 cut(s) 644
AccB1I GGYRCC 1 cut(s) 429
AccB7I CCANNNNNTGG 1 cut(s) 539
AccII CGCG 1 cut(s) 834
AciI CCGC 6 cut(s) 44, 54, 426, 625, 827, 832
AclI AACGTT 1 cut(s) 135
AclWI GGATC 3 cut(s) 20, 33, 455
AcsI RAATTY 1 cut(s) 182
AcuI CTGAAG 1 cut(s) 375
AfiI CCNNNNNNNGG 1 cut(s) 539
AgsI TTSAA 6 cut(s) 65, 160, 178, 200, 633, 746
AhlI ACTAGT 1 cut(s) 277
AjuI GAANNNNNNNTTGG 2 cut(s) 526, 558
AluBI AGCT 5 cut(s) 149, 359, 404, 515, 605
AluI AGCT 5 cut(s) 149, 359, 404, 515, 605
Alw26I GTCTC 2 cut(s) 329, 567
AlwI GGATC 3 cut(s) 20, 33, 455
AoxI GGCC 2 cut(s) 216, 828
ApeKI GCWGC 6 cut(s) 47, 149, 254, 323, 614, 725
ApoI RAATTY 1 cut(s) 182
AspLEI GCGC 1 cut(s) 836
AspS9I GGNCC 4 cut(s) 216, 341, 708, 829
AsuHPI GGTGA 2 cut(s) 68, 245
AsuII TTCGAA 1 cut(s) 376
AvaII GGWCC 2 cut(s) 341, 708
BaeGI GKGCMC 1 cut(s) 432
BamHI GGATCC 1 cut(s) 25
BanI GGYRCC 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 122
BbvI GCAGC 6 cut(s) 34, 136, 241, 335, 626, 737
BccI CCATC 2 cut(s) 80, 352
BclI TGATCA 1 cut(s) 681
BcoDI GTCTC 2 cut(s) 329, 567
BcuI ACTAGT 1 cut(s) 277
BfaI CTAG 2 cut(s) 258, 278
BfuAI ACCTGC 1 cut(s) 644
BisI GCNGC 8 cut(s) 45, 48, 150, 255, 324, 615, 726, 828
BlsI GCNGC 8 cut(s) 46, 49, 151, 256, 325, 616, 727, 829
Bme18I GGWCC 2 cut(s) 341, 708
BmgT120I GGNCC 4 cut(s) 216, 341, 708, 829
BmiI GGNNCC 3 cut(s) 27, 431, 710
BmsI GCATC 3 cut(s) 310, 340, 578
BpiI GAAGAC 1 cut(s) 122
BpmI CTGGAG 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 376
Bsa29I ATCGAT 1 cut(s) 294
BsaI GGTCTC 1 cut(s) 329
BsaWI WCCGGW 1 cut(s) 338
Bsc4I CCNNNNNNNGG 1 cut(s) 539
Bse3DI GCAATG 1 cut(s) 249
BseCI ATCGAT 1 cut(s) 294
BseGI GGATG 2 cut(s) 344, 463
BseLI CCNNNNNNNGG 1 cut(s) 539
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 1 cut(s) 167
BseRI GAGGAG 1 cut(s) 347
BseSI GKGCMC 1 cut(s) 432
BseXI GCAGC 6 cut(s) 34, 136, 241, 335, 626, 737
Bsh1236I CGCG 1 cut(s) 834
BshFI GGCC 2 cut(s) 218, 830
BshNI GGYRCC 1 cut(s) 429
BshVI ATCGAT 1 cut(s) 294
BsiSI CCGG 1 cut(s) 339
BslI CCNNNNNNNGG 1 cut(s) 539
BsmAI GTCTC 2 cut(s) 329, 567
BsmI GAATGC 1 cut(s) 728
BsnI GGCC 2 cut(s) 218, 830
Bso31I GGTCTC 1 cut(s) 329
Bsp119I TTCGAA 1 cut(s) 376
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 4 cut(s) 25, 295, 460, 681
BspACI CCGC 6 cut(s) 44, 54, 426, 625, 827, 832
BspANI GGCC 2 cut(s) 218, 830
BspCNI CTCAG 1 cut(s) 166
BspDI ATCGAT 1 cut(s) 294
BspFNI CGCG 1 cut(s) 834
BspLI GGNNCC 3 cut(s) 27, 431, 710
BspMI ACCTGC 1 cut(s) 644
BspPI GGATC 3 cut(s) 20, 33, 455
BspQI GCTCTTC 1 cut(s) 822
BspT104I TTCGAA 1 cut(s) 376
BspT107I GGYRCC 1 cut(s) 429
BspTNI GGTCTC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 249
BssMI GATC 4 cut(s) 25, 295, 460, 681
Bst4CI ACNGT 1 cut(s) 171
Bst6I CTCTTC 1 cut(s) 822
BstBI TTCGAA 1 cut(s) 376
BstC8I GCNNGC 4 cut(s) 52, 428, 612, 832
BstDEI CTNAG 2 cut(s) 153, 511
BstF5I GGATG 2 cut(s) 344, 463
BstFNI CGCG 1 cut(s) 834
BstHHI GCGC 1 cut(s) 836
BstKTI GATC 4 cut(s) 28, 298, 463, 684
BstMAI GTCTC 2 cut(s) 329, 567
BstMBI GATC 4 cut(s) 25, 295, 460, 681
BstMWI GCNNNNNNNGC 2 cut(s) 611, 833
BstNSI RCATGY 3 cut(s) 54, 413, 614
BstSLI GKGCMC 1 cut(s) 432
BstUI CGCG 1 cut(s) 834
BstV1I GCAGC 6 cut(s) 34, 136, 241, 335, 626, 737
BstV2I GAAGAC 1 cut(s) 122
BstX2I RGATCY 1 cut(s) 25
BstXI CCANNNNNNTGG 2 cut(s) 22, 213
BstYI RGATCY 1 cut(s) 25
Bsu15I ATCGAT 1 cut(s) 294
BsuRI GGCC 2 cut(s) 218, 830
BsuTUI ATCGAT 1 cut(s) 294
BtsCI GGATG 2 cut(s) 344, 463
BtsIMutI CAGTG 1 cut(s) 16
BveI ACCTGC 1 cut(s) 644
Cac8I GCNNGC 4 cut(s) 52, 428, 612, 832
CfoI GCGC 1 cut(s) 836
Cfr13I GGNCC 4 cut(s) 216, 341, 708, 829
ClaI ATCGAT 1 cut(s) 294
CviAII CATG 6 cut(s) 22, 51, 244, 410, 611, 821
CviJI RGCY 9 cut(s) 47, 128, 149, 218, 359, 404, 515, 605, 830
CviKI_1 RGCY 9 cut(s) 47, 128, 149, 218, 359, 404, 515, 605, 830
DdeI CTNAG 2 cut(s) 153, 511
DpnI GATC 4 cut(s) 27, 297, 462, 683
DpnII GATC 4 cut(s) 25, 295, 460, 681
Eam1104I CTCTTC 1 cut(s) 822
EarI CTCTTC 1 cut(s) 822
Eco31I GGTCTC 1 cut(s) 329
Eco47I GGWCC 2 cut(s) 341, 708
Eco57I CTGAAG 1 cut(s) 375
EcoRI GAATTC 1 cut(s) 182
EcoT22I ATGCAT 1 cut(s) 23
FaeI CATG 6 cut(s) 25, 54, 247, 413, 614, 824
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FatI CATG 6 cut(s) 21, 50, 243, 409, 610, 820
FauI CCCGC 3 cut(s) 419, 632, 839
FauNDI CATATG 1 cut(s) 17
FbaI TGATCA 1 cut(s) 681
Fnu4HI GCNGC 8 cut(s) 45, 48, 150, 255, 324, 615, 726, 828
FokI GGATG 2 cut(s) 331, 470
Fsp4HI GCNGC 8 cut(s) 45, 48, 150, 255, 324, 615, 726, 828
FspBI CTAG 2 cut(s) 258, 278
GlaI GCGC 1 cut(s) 835
GluI GCNGC 8 cut(s) 45, 48, 150, 255, 324, 615, 726, 828
GsuI CTGGAG 1 cut(s) 549
HaeIII GGCC 2 cut(s) 218, 830
HapII CCGG 1 cut(s) 339
HhaI GCGC 1 cut(s) 836
Hin1II CATG 6 cut(s) 25, 54, 247, 413, 614, 824
Hin6I GCGC 1 cut(s) 834
HinP1I GCGC 1 cut(s) 834
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 2 cut(s) 196, 497
HpaII CCGG 1 cut(s) 339
HphI GGTGA 2 cut(s) 68, 245
Hpy166II GTNNAC 1 cut(s) 787
Hpy188I TCNGA 5 cut(s) 114, 195, 349, 391, 734
Hpy188III TCNNGA 2 cut(s) 464, 674
Hpy8I GTNNAC 1 cut(s) 787
HpyCH4III ACNGT 1 cut(s) 171
HpyCH4IV ACGT 1 cut(s) 135
HpyF10VI GCNNNNNNNGC 2 cut(s) 611, 833
HpyF3I CTNAG 2 cut(s) 153, 511
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 6 cut(s) 25, 54, 247, 413, 614, 824
HspAI GCGC 1 cut(s) 834
Ksp22I TGATCA 1 cut(s) 681
Kzo9I GATC 4 cut(s) 25, 295, 460, 681
LguI GCTCTTC 1 cut(s) 822
LpnPI CCDG 5 cut(s) 352, 477, 579, 649, 687
Lsp1109I GCAGC 6 cut(s) 34, 136, 241, 335, 626, 737
LweI GCATC 3 cut(s) 310, 340, 578
MaeI CTAG 2 cut(s) 258, 278
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 3 cut(s) 233, 369, 619
MalI GATC 4 cut(s) 27, 297, 462, 683
MboI GATC 4 cut(s) 25, 295, 460, 681
MboII GAAGA 4 cut(s) 127, 131, 166, 809
MflI RGATCY 1 cut(s) 25
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 8 cut(s) 65, 182, 385, 467, 537, 628, 677, 750
MmeI TCCRAC 1 cut(s) 686
MnlI CCTC 4 cut(s) 299, 325, 577, 728
Mph1103I ATGCAT 1 cut(s) 23
MseI TTAA 3 cut(s) 420, 452, 591
MslI CAYNNNNRTG 3 cut(s) 20, 242, 435
MspI CCGG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 728
MvnI CGCG 1 cut(s) 834
MwoI GCNNNNNNNGC 2 cut(s) 611, 833
NdeI CATATG 1 cut(s) 17
NdeII GATC 4 cut(s) 25, 295, 460, 681
NlaIII CATG 6 cut(s) 25, 54, 247, 413, 614, 824
NlaIV GGNNCC 3 cut(s) 27, 431, 710
NmuCI GTSAC 1 cut(s) 233
NsiI ATGCAT 1 cut(s) 23
NspI RCATGY 3 cut(s) 54, 413, 614
NspV TTCGAA 1 cut(s) 376
PaeI GCATGC 2 cut(s) 54, 614
PciSI GCTCTTC 1 cut(s) 822
PctI GAATGC 1 cut(s) 728
PfeI GAWTC 2 cut(s) 196, 497
PflMI CCANNNNNTGG 1 cut(s) 539
PkrI GCNGC 8 cut(s) 46, 49, 151, 256, 325, 616, 727, 829
PsiI TTATAA 1 cut(s) 363
Psp1406I AACGTT 1 cut(s) 135
PspN4I GGNNCC 3 cut(s) 27, 431, 710
PspPI GGNCC 4 cut(s) 216, 341, 708, 829
PsuI RGATCY 1 cut(s) 25
RseI CAYNNNNRTG 3 cut(s) 20, 242, 435
SapI GCTCTTC 1 cut(s) 822
SaqAI TTAA 3 cut(s) 420, 452, 591
SatI GCNGC 8 cut(s) 45, 48, 150, 255, 324, 615, 726, 828
Sau3AI GATC 4 cut(s) 25, 295, 460, 681
Sau96I GGNCC 4 cut(s) 216, 341, 708, 829
SduI GDGCHC 1 cut(s) 432
SfaNI GCATC 3 cut(s) 310, 340, 578
SfuI TTCGAA 1 cut(s) 376
SinI GGWCC 2 cut(s) 341, 708
SmiMI CAYNNNNRTG 3 cut(s) 20, 242, 435
SpeI ACTAGT 1 cut(s) 277
SphI GCATGC 2 cut(s) 54, 614
Sse9I AATT 8 cut(s) 65, 182, 385, 467, 537, 628, 677, 750
SsiI CCGC 6 cut(s) 44, 54, 426, 625, 827, 832
SspMI CTAG 2 cut(s) 258, 278
TaaI ACNGT 1 cut(s) 171
TaiI ACGT 1 cut(s) 138
TaqI TCGA 3 cut(s) 294, 376, 582
TasI AATT 8 cut(s) 65, 182, 385, 467, 537, 628, 677, 750
TauI GCSGC 2 cut(s) 47, 830
TfiI GAWTC 2 cut(s) 196, 497
Tru1I TTAA 3 cut(s) 420, 452, 591
Tru9I TTAA 3 cut(s) 420, 452, 591
TscAI CASTG 1 cut(s) 16
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 6 cut(s) 47, 149, 254, 323, 614, 725
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 4 cut(s) 54, 510, 711, 809
TspRI CASTG 1 cut(s) 16
Van91I CCANNNNNTGG 1 cut(s) 539
VpaK11BI GGWCC 2 cut(s) 341, 708
XapI RAATTY 1 cut(s) 182
XceI RCATGY 3 cut(s) 54, 413, 614
XspI CTAG 2 cut(s) 258, 278
Zsp2I ATGCAT 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.