Rw5G017420

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000319
Physical Location & Seq
Forward (+)
22169317 .. 22171718
2402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G017420.1

Sequence Viewer

Length: 1263 bp
ATGGATCCTTCTATGTATAGGGCGGCAACTTGTGGTGATGTTGGTTTCTTGCAAAGAATTAGAGATGGTGATGTATCAACTGATCTTCTCAGTCAAAAAACCCCAAAGGGCAACAACATTCTTCACCTTGCAGCTGAATTCAAGCAGATCAATTTCTTCAAAGGCGTCCCTTCCTTTGAGAAAGGCTCTCCGCTGCTTTGGAACATCAACAAGATGGGTGATACCCCCTTGCACATTGCTGCAAGAGTAGGATGTGTTGAATTGGTGGACTTCCTTATTGATCGTGCAAAAATGCTACATGTTCAGAGAGTTGGCGATCAGGAAAGGGGAACAACATATGCTGAATCTTATAAAAAGTTACTGCGGAAGACCAACTTGGAAAAGCATACGGCATTGCATGTTGCTGCCCGATATGGCCATCGTCAAGTGGTGATTTTGTTGATAAAAGCGGATCCTGAATTGTGTTGTTCTACTAATAGCAAAAAGGAGTCGCCATTGTTCTTGGCGATCTGCAAGGGTTTTTCTAACATTGCTGGTGACATTTTGGAGGAGCCTTCAATAAGACCTTCTTTCCAAGGAATCAATGGTGTTACAGCCTTACATGCAGCCGTAACTCATACATACCAAGGCTCTGAAGAGATTGTGAAGATCATGGTGTTCAAATATCCTGATATGATCAAAAAAGCTGATGAACTTGGGTGGACACCCTTACACTACGCAGCATTTAGAGGGAGCGATAAAGCCGTTACATGGTTGATGGAATCAGACAAGTCTAGTACTTCCTATATCTCGGACAAATGTGGAATGACAGCTCTTCATGTCGCAGCATATAAAGGCCACATCGAAGTAATGAAGAAACTGATTCAATATCAGCCTGATACTTGTGAGTGCCTCAATGCCAAAGACCAAACGCTTCTTCATGTCGCAGTTTTAGGTGCACAAAGTGATGTTGTGAAGTACATACTGGAGCTACCCGAGCTTCCGAGACTTATAGATCAAGCAGATAAAGAAGGACATACCCCTTTGCATCTAGCTGTCATGGGTCAAAAGCATGAAATTACAAGAATGCTGAAACGGGACTGTGGAGTCGACAATACTTCTCTTCATAAGGGATTCTCTTTTGACAATTTTCTTGGTGAAGATACCAAAATACAGGTACAGGTGTTAAACTCAGACAATATGAAGATACTATGTGGGATGTTGTTGGCACTCCCAATTAACTGTCATTTTTTACAAAAATTAACCAACATTTATTATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

420

Amino Acids

47.02

Weight (kDa)

7.94

Isoelectric Point (pI)

31.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 8 - 95 8.8e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 119 - 180 2.8e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 194 - 256 4.5e-09 Ankyrin repeats (3 copies)
Ank_4 PF13637 237 - 288 7.9e-10 Ankyrin repeats (many copies)
Ank_2 PF12796 264 - 323 4e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 272 - 321 3e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 303 - 365 9e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AasI GACNNNNNNGTC 1 cut(s) 1085
AccI GTMKAC 1 cut(s) 1089
AciI CCGC 4 cut(s) 23, 191, 364, 449
AclWI GGATC 3 cut(s) 12, 446, 459
AcoI YGGCCR 1 cut(s) 415
AcsI RAATTY 1 cut(s) 137
AcuI CTGAAG 1 cut(s) 654
AcyI GRCGYC 1 cut(s) 165
AdeI CACNNNGTG 1 cut(s) 944
AfaI GTAC 3 cut(s) 778, 959, 1158
AfiI CCNNNNNNNGG 1 cut(s) 750
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 6 cut(s) 142, 160, 260, 558, 661, 866
AjuI GAANNNNNNNTTGG 4 cut(s) 359, 391, 900, 932
AluBI AGCT 6 cut(s) 134, 686, 812, 970, 979, 1034
AluI AGCT 6 cut(s) 134, 686, 812, 970, 979, 1034
Alw21I GWGCWC 1 cut(s) 940
Alw26I GTCTC 1 cut(s) 979
Alw44I GTGCAC 1 cut(s) 936
AlwI GGATC 3 cut(s) 12, 446, 459
Ama87I CYCGRG 1 cut(s) 974
AoxI GGCC 2 cut(s) 415, 835
ApaLI GTGCAC 1 cut(s) 936
ApeKI GCWGC 7 cut(s) 131, 193, 239, 404, 605, 719, 824
ApoI RAATTY 1 cut(s) 137
AsuHPI GGTGA 7 cut(s) 47, 80, 116, 230, 442, 548, 1148
AvaI CYCGRG 1 cut(s) 974
BaeGI GKGCMC 1 cut(s) 940
BaeI ACNNNNGTAYC 2 cut(s) 1148, 1181
BalI TGGCCA 1 cut(s) 417
BamHI GGATCC 2 cut(s) 4, 451
BbsI GAAGAC 1 cut(s) 374
Bbv12I GWGCWC 1 cut(s) 940
BbvI GCAGC 7 cut(s) 143, 180, 226, 391, 617, 731, 836
BccI CCATC 4 cut(s) 59, 208, 426, 751
BceAI ACGGC 3 cut(s) 405, 593, 728
BclI TGATCA 1 cut(s) 675
BcoDI GTCTC 1 cut(s) 979
BfaI CTAG 2 cut(s) 774, 1031
BisI GCNGC 8 cut(s) 24, 132, 194, 240, 405, 606, 720, 825
BlsI GCNGC 8 cut(s) 25, 133, 195, 241, 406, 607, 721, 826
BmcAI AGTACT 1 cut(s) 778
BmeT110I CYCGRG 1 cut(s) 974
BmiI GGNNCC 3 cut(s) 6, 453, 552
BmsI GCATC 1 cut(s) 1036
BpiI GAAGAC 1 cut(s) 374
BplI GAGNNNNNCTC 2 cut(s) 170, 202
BpmI CTGGAG 1 cut(s) 986
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 2 cut(s) 574, 625
Bsc4I CCNNNNNNNGG 1 cut(s) 750
Bse1I ACTGG 1 cut(s) 969
Bse3DI GCAATG 3 cut(s) 234, 392, 528
BseDI CCNNGG 2 cut(s) 574, 625
BseGI GGATG 2 cut(s) 257, 1203
BseLI CCNNNNNNNGG 1 cut(s) 750
BseMI GCAATG 3 cut(s) 234, 392, 528
BseMII CTCAG 2 cut(s) 103, 1185
BseNI ACTGG 1 cut(s) 969
BseRI GAGGAG 1 cut(s) 563
BseSI GKGCMC 1 cut(s) 940
BseXI GCAGC 7 cut(s) 143, 180, 226, 391, 617, 731, 836
BshFI GGCC 2 cut(s) 417, 837
BsiHKAI GWGCWC 1 cut(s) 940
BsiHKCI CYCGRG 1 cut(s) 974
BslFI GGGAC 2 cut(s) 152, 1091
BslI CCNNNNNNNGG 1 cut(s) 750
BsmAI GTCTC 1 cut(s) 979
BsmFI GGGAC 2 cut(s) 152, 1091
BsmI GAATGC 1 cut(s) 1071
BsnI GGCC 2 cut(s) 417, 837
BsoBI CYCGRG 1 cut(s) 974
Bsp1286I GDGCHC 1 cut(s) 940
BspACI CCGC 4 cut(s) 23, 191, 364, 449
BspANI GGCC 2 cut(s) 417, 837
BspCNI CTCAG 2 cut(s) 102, 1184
BspLI GGNNCC 3 cut(s) 6, 453, 552
BspPI GGATC 3 cut(s) 12, 446, 459
BspQI GCTCTTC 1 cut(s) 819
BsrDI GCAATG 3 cut(s) 234, 392, 528
BsrI ACTGG 1 cut(s) 969
BssECI CCNNGG 2 cut(s) 574, 625
BssNI GRCGYC 1 cut(s) 165
BssT1I CCWWGG 2 cut(s) 574, 625
Bst4CI ACNGT 2 cut(s) 1082, 1223
Bst6I CTCTTC 3 cut(s) 630, 819, 1107
BstACI GRCGYC 1 cut(s) 165
BstDEI CTNAG 2 cut(s) 89, 1171
BstF5I GGATG 2 cut(s) 257, 1203
BstMAI GTCTC 1 cut(s) 979
BstMWI GCNNNNNNNGC 2 cut(s) 602, 976
BstNSI RCATGY 3 cut(s) 302, 401, 605
BstSLI GKGCMC 1 cut(s) 940
BstV1I GCAGC 7 cut(s) 143, 180, 226, 391, 617, 731, 836
BstV2I GAAGAC 1 cut(s) 374
BstX2I RGATCY 2 cut(s) 4, 451
BstYI RGATCY 2 cut(s) 4, 451
BsuRI GGCC 2 cut(s) 417, 837
BtsCI GGATG 2 cut(s) 257, 1203
CseI GACGC 1 cut(s) 154
Csp6I GTAC 3 cut(s) 777, 958, 1157
CviAII CATG 9 cut(s) 299, 398, 602, 652, 750, 818, 920, 1039, 1052
CviQI GTAC 3 cut(s) 777, 958, 1157
DdeI CTNAG 2 cut(s) 89, 1171
DraIII CACNNNGTG 1 cut(s) 944
DrdI GACNNNNNNGTC 1 cut(s) 1085
DseDI GACNNNNNNGTC 1 cut(s) 1085
EaeI YGGCCR 1 cut(s) 415
Eam1104I CTCTTC 3 cut(s) 630, 819, 1107
EarI CTCTTC 3 cut(s) 630, 819, 1107
Eco130I CCWWGG 2 cut(s) 574, 625
Eco57I CTGAAG 1 cut(s) 654
Eco88I CYCGRG 1 cut(s) 974
EcoRI GAATTC 1 cut(s) 137
EcoT14I CCWWGG 2 cut(s) 574, 625
ErhI CCWWGG 2 cut(s) 574, 625
FaeI CATG 9 cut(s) 302, 401, 605, 655, 753, 821, 923, 1042, 1055
FalI AAGNNNNNCTT 4 cut(s) 359, 391, 553, 585
FaqI GGGAC 2 cut(s) 152, 1091
FatI CATG 9 cut(s) 298, 397, 601, 651, 749, 817, 919, 1038, 1051
FauNDI CATATG 1 cut(s) 337
FbaI TGATCA 1 cut(s) 675
FblI GTMKAC 1 cut(s) 1089
Fnu4HI GCNGC 8 cut(s) 24, 132, 194, 240, 405, 606, 720, 825
FokI GGATG 2 cut(s) 264, 1210
Fsp4HI GCNGC 8 cut(s) 24, 132, 194, 240, 405, 606, 720, 825
FspBI CTAG 2 cut(s) 774, 1031
GluI GCNGC 8 cut(s) 24, 132, 194, 240, 405, 606, 720, 825
GsuI CTGGAG 1 cut(s) 986
HaeIII GGCC 2 cut(s) 417, 837
HgaI GACGC 1 cut(s) 154
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 9 cut(s) 302, 401, 605, 655, 753, 821, 923, 1042, 1055
HincII GTYRAC 1 cut(s) 1090
HindII GTYRAC 1 cut(s) 1090
HinfI GANTC 7 cut(s) 344, 488, 579, 761, 862, 1086, 1113
HphI GGTGA 7 cut(s) 47, 80, 116, 230, 442, 548, 1148
Hpy166II GTNNAC 4 cut(s) 268, 702, 938, 1090
Hpy188I TCNGA 6 cut(s) 306, 634, 766, 793, 984, 1174
Hpy188III TCNNGA 3 cut(s) 320, 455, 668
Hpy8I GTNNAC 4 cut(s) 268, 702, 938, 1090
HpyAV CCTTC 5 cut(s) 18, 180, 564, 576, 1004
HpyCH4III ACNGT 2 cut(s) 1082, 1223
HpyF10VI GCNNNNNNNGC 2 cut(s) 602, 976
HpyF3I CTNAG 2 cut(s) 89, 1171
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 9 cut(s) 302, 401, 605, 655, 753, 821, 923, 1042, 1055
Ksp22I TGATCA 1 cut(s) 675
LguI GCTCTTC 1 cut(s) 819
LmnI GCTCC 3 cut(s) 550, 732, 967
LpnPI CCDG 8 cut(s) 305, 468, 519, 681, 888, 950, 1139, 1145
Lsp1109I GCAGC 7 cut(s) 143, 180, 226, 391, 617, 731, 836
LweI GCATC 1 cut(s) 1036
MaeI CTAG 2 cut(s) 774, 1031
MaeIII GTNAC 5 cut(s) 357, 536, 589, 610, 745
MflI RGATCY 2 cut(s) 4, 451
MhlI GDGCHC 1 cut(s) 940
MlsI TGGCCA 1 cut(s) 417
MluCI AATT 9 cut(s) 57, 137, 151, 260, 458, 1056, 1126, 1215, 1238
MluNI TGGCCA 1 cut(s) 417
MlyI GAGTC 2 cut(s) 497, 1095
MnlI CCTC 3 cut(s) 541, 722, 902
Mox20I TGGCCA 1 cut(s) 417
MscI TGGCCA 1 cut(s) 417
MseI TTAA 4 cut(s) 1166, 1218, 1241, 1261
Msp20I TGGCCA 1 cut(s) 417
MspA1I CMGCKG 2 cut(s) 134, 193
Mva1269I GAATGC 1 cut(s) 1071
MwoI GCNNNNNNNGC 2 cut(s) 602, 976
NdeI CATATG 1 cut(s) 337
NlaIII CATG 9 cut(s) 302, 401, 605, 655, 753, 821, 923, 1042, 1055
NlaIV GGNNCC 3 cut(s) 6, 453, 552
NmuCI GTSAC 1 cut(s) 536
NspI RCATGY 3 cut(s) 302, 401, 605
PciI ACATGT 1 cut(s) 298
PciSI GCTCTTC 1 cut(s) 819
PctI GAATGC 1 cut(s) 1071
PfeI GAWTC 5 cut(s) 344, 579, 761, 862, 1113
PkrI GCNGC 8 cut(s) 25, 133, 195, 241, 406, 607, 721, 826
PleI GAGTC 2 cut(s) 496, 1094
PpsI GAGTC 2 cut(s) 496, 1094
PscI ACATGT 1 cut(s) 298
PsiI TTATAA 1 cut(s) 351
PspN4I GGNNCC 3 cut(s) 6, 453, 552
PsuI RGATCY 2 cut(s) 4, 451
PvuII CAGCTG 1 cut(s) 134
RsaI GTAC 3 cut(s) 778, 959, 1158
RsaNI GTAC 3 cut(s) 777, 958, 1157
SalI GTCGAC 1 cut(s) 1088
SapI GCTCTTC 1 cut(s) 819
SaqAI TTAA 4 cut(s) 1166, 1218, 1241, 1261
SatI GCNGC 8 cut(s) 24, 132, 194, 240, 405, 606, 720, 825
ScaI AGTACT 1 cut(s) 778
SchI GAGTC 2 cut(s) 497, 1095
SduI GDGCHC 1 cut(s) 940
SfaNI GCATC 1 cut(s) 1036
Sse9I AATT 9 cut(s) 57, 137, 151, 260, 458, 1056, 1126, 1215, 1238
SsiI CCGC 4 cut(s) 23, 191, 364, 449
SspMI CTAG 2 cut(s) 774, 1031
StyI CCWWGG 2 cut(s) 574, 625
TaaI ACNGT 2 cut(s) 1082, 1223
TaqI TCGA 2 cut(s) 843, 1089
TasI AATT 9 cut(s) 57, 137, 151, 260, 458, 1056, 1126, 1215, 1238
TatI WGTACW 2 cut(s) 776, 957
TauI GCSGC 1 cut(s) 26
TfiI GAWTC 5 cut(s) 344, 579, 761, 862, 1113
Tru1I TTAA 4 cut(s) 1166, 1218, 1241, 1261
Tru9I TTAA 4 cut(s) 1166, 1218, 1241, 1261
TseFI GTSAC 1 cut(s) 536
TseI GCWGC 7 cut(s) 131, 193, 239, 404, 605, 719, 824
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 7 cut(s) 705, 806, 866, 908, 1068, 1094, 1196
VneI GTGCAC 1 cut(s) 936
XapI RAATTY 1 cut(s) 137
XceI RCATGY 3 cut(s) 302, 401, 605
XcmI CCANNNNNNNNNTGG 1 cut(s) 581
XmiI GTMKAC 1 cut(s) 1089
XspI CTAG 2 cut(s) 774, 1031
ZrmI AGTACT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.