MD05G1213100.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000319
Physical Location & Seq
Reverse (-)
34440257 .. 34441937
1681 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1213100.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGAATCCTTCTCTGTATGAAGCAGCTGCAACTGGTGATGTTGGCTTCTTGGAAGGACTTCTAACAGCTGATCTCTCTCAGAAAACACCTAAAGAGAACAATATTCTTCACATTGCAGCTGAATTCAAGCAAATAAACTTCTTCAAAAAAGTGAAAAAAATTCCCGAATCTCCCCGGTTTTGGGCCACCAACAAGAATGGCGAAACTCCTCTACATGTTGCCGCGAGAGTAGGTTGCGGTGAAGTAGTAACGTTCCTCATTGAGCACATAAAAGCGGAACCTATGGAAGTGGTTGATCTTGATCCAGAAAAGGAACCAACTGACGGTGAAGCTTACAAAAAGCTACTCCGAATGCCTAATTTGGAAATGGATACAGCACTGCATGTTGCTGTTCGATACAATCATGCTGGAGTAGCGAAGATTTTGCTGAGTGCTGATCCTGAATTGTGTTGTTCATTTTACAGCACCAAGGAGTCACCCCTGTTCCTAGCTGTTAGAGCCGGGTCTACCAGCATTGCTGATTATATTTTAAAAAAGACTCCGGATCATGAATCTCTTTCTTTTCAGGGAACAAATGGTGTGACCGCTTTGCACGCAGCAGCCACTCGTATACCCTTCGCCAAGAAAGGAATTGTGCAATCTATGGTTACCAAAAATCCTGGGATGGTCGAAGTTCGTGATGAAATAGGGTGGACTCCATTACACTACGCATCACTGAGAGGGAACCTTAAAGCAGCTCAACTACTGATTCAAAATTGCAAAACTTTCAAATTTGCTTGTTACATCAAAGACGAGCTTGGCATGTCAGCTCTCCACGTCGCGGCATATGCAGGGCACACCCAAATAATTGAAGAGTTGATCGGACGTTGTCCTGATATTTGTGATTGTGTCAATCACAAGGGCCAAACAGCTCTACATGCAGCAGTTTTAGGCGAAAAGATAAATGTTGTGAAGTACGTTTTGAAGACGCCTAGGCTTGCGAGACTTATAAACGAAGCAGATAATGATGGAAACACTCCTTGGCATCTAGCTGCTATTCATGAAAATAGTGAAATCGTTGCTATTTTAAGGAGAGACAGGAGGTTGAACAGAACTGCTATCAATAAAGAATTTTTGCAAGTTAGCGACATTTTGCTCGCCGAGAACACGGGAAGAATCTTTTATTTATTTCCCTTGGATTTCAACTTTGAACTCTCTATTTTATTTTCTTATATTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

407

Amino Acids

45.07

Weight (kDa)

6.71

Isoelectric Point (pI)

31.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 93 9.1e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 113 - 182 3.9e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 127 - 201 3.8e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 189 - 252 2e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 207 - 288 2e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 262 - 322 5.3e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 271 - 357 1.5e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 271 - 320 1e-07 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 989
AccI GTMKAC 2 cut(s) 506, 610
AccII CGCG 2 cut(s) 224, 821
AccIII TCCGGA 1 cut(s) 541
AciI CCGC 5 cut(s) 222, 237, 275, 585, 821
AclI AACGTT 1 cut(s) 251
AclWI GGATC 3 cut(s) 296, 431, 552
AcsI RAATTY 4 cut(s) 122, 159, 770, 1109
AcyI GRCGYC 1 cut(s) 968
AfaI GTAC 1 cut(s) 956
AfiI CCNNNNNNNGG 4 cut(s) 180, 181, 323, 820
AflIII ACRYGT 1 cut(s) 214
AgsI TTSAA 9 cut(s) 127, 145, 752, 769, 851, 964, 1087, 1183, 1190
AjiI CACGTC 1 cut(s) 817
AjnI CCWGG 1 cut(s) 658
AjuI GAANNNNNNNTTGG 2 cut(s) 1003, 1035
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 2 cut(s) 976, 1068
AlwI GGATC 3 cut(s) 296, 431, 552
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 2 cut(s) 183, 901
ApeKI GCWGC 8 cut(s) 23, 26, 116, 596, 599, 734, 920, 1031
ApoI RAATTY 4 cut(s) 122, 159, 770, 1109
Asp700I GAANNNNTTC 1 cut(s) 57
AspA2I CCTAGG 1 cut(s) 971
AspS9I GGNCC 2 cut(s) 183, 901
AsuC2I CCSGG 2 cut(s) 175, 502
AsuHPI GGTGA 4 cut(s) 47, 251, 338, 468
AvrII CCTAGG 1 cut(s) 971
BaeGI GKGCMC 1 cut(s) 837
BbsI GAAGAC 1 cut(s) 971
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 8 cut(s) 13, 35, 128, 608, 611, 746, 932, 1018
BccI CCATC 2 cut(s) 658, 1001
BcgI CGANNNNNNTGC 2 cut(s) 406, 440
BciT130I CCWGG 1 cut(s) 660
BciVI GTATCC 1 cut(s) 364
BcnI CCSGG 2 cut(s) 175, 502
BcoDI GTCTC 2 cut(s) 976, 1068
BfaI CTAG 3 cut(s) 488, 972, 1028
BfuI GTATCC 1 cut(s) 364
BlnI CCTAGG 1 cut(s) 971
Bme1390I CCNGG 3 cut(s) 175, 502, 660
BmgBI CACGTC 1 cut(s) 817
BmgT120I GGNCC 2 cut(s) 183, 901
BmiI GGNNCC 3 cut(s) 279, 315, 725
BmrFI CCNGG 3 cut(s) 175, 502, 660
BmsI GCATC 2 cut(s) 719, 1033
BpiI GAAGAC 1 cut(s) 971
BpmI CTGGAG 1 cut(s) 429
BpuMI CCSGG 2 cut(s) 175, 502
BsaBI GATNNNNATC 1 cut(s) 300
BsaHI GRCGYC 1 cut(s) 968
BsaJI CCNNGG 6 cut(s) 173, 468, 659, 971, 1019, 1173
BsaWI WCCGGW 1 cut(s) 541
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 181, 323, 820
Bse1I ACTGG 1 cut(s) 37
Bse3DI GCAATG 2 cut(s) 111, 513
Bse8I GATNNNNATC 1 cut(s) 300
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 1 cut(s) 660
BseDI CCNNGG 6 cut(s) 173, 468, 659, 971, 1019, 1173
BseGI GGATG 1 cut(s) 669
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 4 cut(s) 180, 181, 323, 820
BseMI GCAATG 2 cut(s) 111, 513
BseMII CTCAG 3 cut(s) 92, 419, 707
BseNI ACTGG 1 cut(s) 37
BseRI GAGGAG 1 cut(s) 198
BseSI GKGCMC 1 cut(s) 837
BseXI GCAGC 8 cut(s) 13, 35, 128, 608, 611, 746, 932, 1018
Bsh1236I CGCG 2 cut(s) 224, 821
BshFI GGCC 2 cut(s) 185, 903
BsiHKAI GWGCWC 1 cut(s) 267
BsiSI CCGG 3 cut(s) 175, 501, 542
BslI CCNNNNNNNGG 4 cut(s) 180, 181, 323, 820
BsmAI GTCTC 2 cut(s) 976, 1068
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 2 cut(s) 185, 903
Bsp1286I GDGCHC 2 cut(s) 267, 837
Bsp13I TCCGGA 1 cut(s) 541
Bsp143I GATC 6 cut(s) 70, 295, 301, 436, 544, 858
BspACI CCGC 5 cut(s) 222, 237, 275, 585, 821
BspANI GGCC 2 cut(s) 185, 903
BspCNI CTCAG 3 cut(s) 91, 420, 708
BspEI TCCGGA 1 cut(s) 541
BspFNI CGCG 2 cut(s) 224, 821
BspHI TCATGA 2 cut(s) 547, 1039
BspLI GGNNCC 3 cut(s) 279, 315, 725
BspPI GGATC 3 cut(s) 296, 431, 552
BsrDI GCAATG 2 cut(s) 111, 513
BsrI ACTGG 1 cut(s) 37
BssECI CCNNGG 6 cut(s) 173, 468, 659, 971, 1019, 1173
BssMI GATC 6 cut(s) 70, 295, 301, 436, 544, 858
BssNAI GTATAC 1 cut(s) 611
BssNI GRCGYC 1 cut(s) 968
BssT1I CCWWGG 4 cut(s) 468, 971, 1019, 1173
Bst1107I GTATAC 1 cut(s) 611
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 1 cut(s) 326
Bst6I CTCTTC 1 cut(s) 846
BstACI GRCGYC 1 cut(s) 968
BstC8I GCNNGC 3 cut(s) 594, 978, 1137
BstDEI CTNAG 3 cut(s) 78, 428, 716
BstEII GGTNACC 1 cut(s) 646
BstF5I GGATG 1 cut(s) 669
BstFNI CGCG 2 cut(s) 224, 821
BstKTI GATC 6 cut(s) 73, 298, 304, 439, 547, 861
BstMAI GTCTC 2 cut(s) 976, 1068
BstMBI GATC 6 cut(s) 70, 295, 301, 436, 544, 858
BstMWI GCNNNNNNNGC 5 cut(s) 413, 497, 593, 827, 917
BstNI CCWGG 1 cut(s) 660
BstNSI RCATGY 4 cut(s) 218, 386, 805, 920
BstPI GGTNACC 1 cut(s) 646
BstSCI CCNGG 3 cut(s) 173, 500, 658
BstSLI GKGCMC 1 cut(s) 837
BstUI CGCG 2 cut(s) 224, 821
BstV1I GCAGC 8 cut(s) 13, 35, 128, 608, 611, 746, 932, 1018
BstV2I GAAGAC 1 cut(s) 971
BstZ17I GTATAC 1 cut(s) 611
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 2 cut(s) 185, 903
BtrI CACGTC 1 cut(s) 817
BtsCI GGATG 1 cut(s) 669
BtsI GCAGTG 1 cut(s) 377
BtsIMutI CAGTG 2 cut(s) 377, 713
Cac8I GCNNGC 3 cut(s) 594, 978, 1137
CciI TCATGA 2 cut(s) 547, 1039
Cfr13I GGNCC 2 cut(s) 183, 901
CseI GACGC 1 cut(s) 976
Csp6I GTAC 1 cut(s) 955
CviAII CATG 7 cut(s) 215, 383, 404, 548, 802, 917, 1040
CviQI GTAC 1 cut(s) 955
DdeI CTNAG 3 cut(s) 78, 428, 716
DpnI GATC 6 cut(s) 72, 297, 303, 438, 546, 860
DpnII GATC 6 cut(s) 70, 295, 301, 436, 544, 858
DraI TTTAAA 1 cut(s) 531
Eam1104I CTCTTC 1 cut(s) 846
EarI CTCTTC 1 cut(s) 846
Eco130I CCWWGG 4 cut(s) 468, 971, 1019, 1173
Eco91I GGTNACC 1 cut(s) 646
EcoO65I GGTNACC 1 cut(s) 646
EcoRI GAATTC 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 658
EcoT14I CCWWGG 4 cut(s) 468, 971, 1019, 1173
ErhI CCWWGG 4 cut(s) 468, 971, 1019, 1173
FaeI CATG 7 cut(s) 218, 386, 407, 551, 805, 920, 1043
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FatI CATG 7 cut(s) 214, 382, 403, 547, 801, 916, 1039
FauNDI CATATG 1 cut(s) 826
FblI GTMKAC 2 cut(s) 506, 610
FokI GGATG 1 cut(s) 676
FspBI CTAG 3 cut(s) 488, 972, 1028
GsuI CTGGAG 1 cut(s) 429
HaeIII GGCC 2 cut(s) 185, 903
HapII CCGG 3 cut(s) 175, 501, 542
HgaI GACGC 1 cut(s) 976
Hin1I GRCGYC 1 cut(s) 968
Hin1II CATG 7 cut(s) 218, 386, 407, 551, 805, 920, 1043
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 8 cut(s) 4, 167, 473, 538, 551, 694, 748, 1155
HpaII CCGG 3 cut(s) 175, 501, 542
HphI GGTGA 4 cut(s) 47, 251, 338, 468
Hpy166II GTNNAC 3 cut(s) 507, 611, 693
Hpy188I TCNGA 3 cut(s) 81, 350, 863
Hpy188III TCNNGA 9 cut(s) 164, 299, 305, 440, 542, 548, 677, 872, 1040
Hpy8I GTNNAC 3 cut(s) 507, 611, 693
Hpy99I CGWCG 1 cut(s) 821
HpyAV CCTTC 3 cut(s) 18, 47, 625
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 4 cut(s) 251, 816, 865, 957
HpyCH4V TGCA 9 cut(s) 29, 116, 382, 592, 637, 759, 830, 920, 1117
HpyF10VI GCNNNNNNNGC 5 cut(s) 413, 497, 593, 827, 917
HpyF3I CTNAG 3 cut(s) 78, 428, 716
HpySE526I ACGT 4 cut(s) 251, 816, 865, 957
Hsp92I GRCGYC 1 cut(s) 968
Hsp92II CATG 7 cut(s) 218, 386, 407, 551, 805, 920, 1043
Kpn2I TCCGGA 1 cut(s) 541
Kzo9I GATC 6 cut(s) 70, 295, 301, 436, 544, 858
Lsp1109I GCAGC 8 cut(s) 13, 35, 128, 608, 611, 746, 932, 1018
LweI GCATC 2 cut(s) 719, 1033
MaeI CTAG 3 cut(s) 488, 972, 1028
MaeII ACGT 4 cut(s) 251, 816, 865, 957
MaeIII GTNAC 5 cut(s) 247, 474, 580, 646, 779
MalI GATC 6 cut(s) 72, 297, 303, 438, 546, 860
MboI GATC 6 cut(s) 70, 295, 301, 436, 544, 858
MboII GAAGA 6 cut(s) 98, 133, 430, 863, 976, 1164
MhlI GDGCHC 2 cut(s) 267, 837
MluCI AATT 9 cut(s) 122, 159, 358, 443, 630, 754, 770, 846, 1109
MlyI GAGTC 3 cut(s) 482, 532, 688
MnlI CCTC 4 cut(s) 219, 266, 713, 1074
MroI TCCGGA 1 cut(s) 541
MroXI GAANNNNTTC 1 cut(s) 57
MseI TTAA 3 cut(s) 530, 729, 1067
MspA1I CMGCKG 3 cut(s) 26, 68, 119
MspI CCGG 3 cut(s) 175, 501, 542
MspR9I CCNGG 3 cut(s) 175, 502, 660
Mva1269I GAATGC 1 cut(s) 357
MvaI CCWGG 1 cut(s) 660
MvnI CGCG 2 cut(s) 224, 821
MwoI GCNNNNNNNGC 5 cut(s) 413, 497, 593, 827, 917
NciI CCSGG 2 cut(s) 175, 502
NdeI CATATG 1 cut(s) 826
NdeII GATC 6 cut(s) 70, 295, 301, 436, 544, 858
NlaIII CATG 7 cut(s) 218, 386, 407, 551, 805, 920, 1043
NlaIV GGNNCC 3 cut(s) 279, 315, 725
NmeAIII GCCGAG 1 cut(s) 1165
NmuCI GTSAC 2 cut(s) 474, 580
NspI RCATGY 4 cut(s) 218, 386, 805, 920
PagI TCATGA 2 cut(s) 547, 1039
PciI ACATGT 1 cut(s) 214
PctI GAATGC 1 cut(s) 357
PdmI GAANNNNTTC 1 cut(s) 57
PfeI GAWTC 5 cut(s) 4, 167, 551, 748, 1155
PflFI GACNNNGTC 1 cut(s) 867
PleI GAGTC 3 cut(s) 481, 532, 688
PpsI GAGTC 3 cut(s) 481, 532, 688
PscI ACATGT 1 cut(s) 214
PsiI TTATAA 1 cut(s) 989
Psp1406I AACGTT 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 658
PspEI GGTNACC 1 cut(s) 646
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 3 cut(s) 279, 315, 725
PspPI GGNCC 2 cut(s) 183, 901
PsyI GACNNNGTC 1 cut(s) 867
PvuII CAGCTG 3 cut(s) 26, 68, 119
RsaI GTAC 1 cut(s) 956
RsaNI GTAC 1 cut(s) 955
SaqAI TTAA 3 cut(s) 530, 729, 1067
Sau3AI GATC 6 cut(s) 70, 295, 301, 436, 544, 858
Sau96I GGNCC 2 cut(s) 183, 901
SchI GAGTC 3 cut(s) 482, 532, 688
ScrFI CCNGG 3 cut(s) 175, 502, 660
SduI GDGCHC 2 cut(s) 267, 837
SfaNI GCATC 2 cut(s) 719, 1033
Sse9I AATT 9 cut(s) 122, 159, 358, 443, 630, 754, 770, 846, 1109
SsiI CCGC 5 cut(s) 222, 237, 275, 585, 821
SspI AATATT 1 cut(s) 103
SspMI CTAG 3 cut(s) 488, 972, 1028
StyD4I CCNGG 3 cut(s) 173, 500, 658
StyI CCWWGG 4 cut(s) 468, 971, 1019, 1173
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 4 cut(s) 254, 819, 868, 960
TaqI TCGA 2 cut(s) 394, 669
TasI AATT 9 cut(s) 122, 159, 358, 443, 630, 754, 770, 846, 1109
TauI GCSGC 2 cut(s) 224, 824
TfiI GAWTC 5 cut(s) 4, 167, 551, 748, 1155
Tru1I TTAA 3 cut(s) 530, 729, 1067
Tru9I TTAA 3 cut(s) 530, 729, 1067
TscAI CASTG 2 cut(s) 384, 720
TseFI GTSAC 2 cut(s) 474, 580
TseI GCWGC 8 cut(s) 23, 26, 116, 596, 599, 734, 920, 1031
Tsp45I GTSAC 2 cut(s) 474, 580
TspDTI ATGAA 7 cut(s) 17, 33, 444, 564, 696, 1028, 1056
TspRI CASTG 2 cut(s) 384, 720
Tth111I GACNNNGTC 1 cut(s) 867
XapI RAATTY 4 cut(s) 122, 159, 770, 1109
XceI RCATGY 4 cut(s) 218, 386, 805, 920
XmaJI CCTAGG 1 cut(s) 971
XmiI GTMKAC 2 cut(s) 506, 610
XmnI GAANNNNTTC 1 cut(s) 57
XspI CTAG 3 cut(s) 488, 972, 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.