pycom11g27250

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000319
Physical Location & Seq
Forward (+)
29567980 .. 29570971
2992 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g27250.1

Sequence Viewer

Length: 1866 bp
ATGGATCCTTTCCTGTATGAAGCAGCAACATCGGGTGATGTTGGTTTTTTGAGAGACAAAATCAACAGTAAAAATGGTGGTGTATCATCAATTGATCTTCTCCTCCATCAGAAAACGCCTAAAGACAACAACATTCTTCATATCTCCGCCGAATCAAAGCAAATAGATTTCTTCAAAAATGTCGATCCAGATCGATATGGTCAACTGTTTTGGGCTCCCAACAAGAAGGGTAACACTCCACTGCACGTTGCTTCAAGAGTAGGCTGTGTCGAAATAGTCAAGCTACTCATCCAAAACGCCACAACAGCGACAGCGCTAAGCCTCGAAGGAGCTGATGAGGAAAGTGGACTAGTGTCACCAGCTGATAAGGGTGACCCGATCGGACGACAACAACTGCTCCGAATGACTAATTTGCAAAAGGATACAGCCATGCATGTTGCCGTCCGGTCAGGTCACCATCAAGTAGTGCTTCTGTTAATGGAATCCGATCCTCAATTGTGTTGTTTCACTAATAGCGCTAACGAGTCACCATTGTTCCTAGCCACCCTCCACTGGTTTCCACCCCTAGCTTGTTCTATTCTAAACCAGTGTCCAACATCTCCTTCTTTTCAGGGAACCAATGGTCTCACATCTTTGCATGTTGCGGCAGCTCGTACGGGTACTGCTCACTCCCTCTTATTTTGTGCTTACGCAAGGCTTGTGAAGATAATGGTGTCCAAAAATCCTGGGATGATGAAAGAAGTTGACACACTTGGGTGGACTCCCTTGCACTATGCAGCTTTGTGGGGCAACTCTGGAGCAGTGTCAATATTGCTGCAAGCTGACAGTTCGGCCTCATACATTCTAGATAAATCTGGATTGTCAGCTCTACATGTCGCGGCGTATGCAGGCCACACCAGTATCATGGAGGAGGTGATTCGATGCCGGCCTGATACTTGTGATTTGCTCAATCACAAAGGCCAAACGGTTCTTCATGCTGCAGTTTTAGGTGGACAAACATCTGTTGTCGAGTACATATTGAAGACGCCGAAGTTTGCAGGACTCATAGATGAAGCGGACGCAGATGGAAACACTCCTTTGCATCTGTCTGCCATTTACAAAGAAGATGGACTTCGAGAAATTTTGACAAACGATCCCAGAGTGAACAAGTCTGCTCTGAATGGAAAATTATACACAGCTTTCGACATTTTGCTTCAAGATCATGACAATTTTACACAGGTTTTCGATACTAGCATTGATCCTCGCTGTGAACTGATCGGCCCTGTGAAATCCATTGGCATGCCATTTCTTCAAGAGCATATCCTTCATCATATCATAGAAATGGAACCTACAGAGAAGGAGAGGTCTAGTGAACCGGCCATTGCAGACAAGAAAGAAAAGCCGCTAGAAGATACAGATGAAGAGTTGTGGAAAAAGTTTGATACGAATCTACTCATAGCGACGCTTATTGCAACTGTCACTTTCACTGCAGCTTTCACCATGCCCGGAGGACTGAAAGATGATGGAACACCGGTTTTACAAGACAAGGCATCATTTCAAGTTTTTCTGTTGTTCGACTCCCTTTCCTTCATTCTCTCAATATTTGTGGTGCTTGTTCATTTAATTACAGCTAATTTAGCTATAAACTTACAAGGTGTGCGGCCAATTTTAATTCATTATTCTGTTATTGGAATGAACATAGCGTTTGCTTCGGCCTTGTTTGCGATGCTTCCCAAGGGTACCCGATGGCTTGGAATTTTGGCCTGTGTAACCTGTGGCTTCATATGCTTGGTAGCCACCCTTATGTACTACTTGCAGAAACGAAGACGGGAAAGGATTATAAAATGGATGTTGTACATGACGAGGACTAGTGCTCGAACAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

622

Amino Acids

68.34

Weight (kDa)

6.31

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 24 - 99 2.1e-06 Ankyrin repeats (3 copies)
Ank PF00023 77 - 100 3.1e-06 Ankyrin repeat
Ank_2 PF12796 194 - 274 1.8e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 227 - 306 1.6e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 290 - 382 1.2e-11 Ankyrin repeats (3 copies)
PGG PF13962 467 - 568 3.4e-22 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1820
Acc65I GGTACC 1 cut(s) 1718
AccB1I GGYRCC 1 cut(s) 1718
AccII CGCG 1 cut(s) 878
AciI CCGC 6 cut(s) 147, 644, 878, 1055, 1382, 1639
AclWI GGATC 5 cut(s) 12, 179, 482, 1127, 1232
AcoI YGGCCR 2 cut(s) 1356, 1640
AcsI RAATTY 2 cut(s) 1119, 1734
AcyI GRCGYC 1 cut(s) 1025
AfaI GTAC 6 cut(s) 655, 661, 1013, 1720, 1787, 1835
AfeI AGCGCT 2 cut(s) 315, 517
AfiI CCNNNNNNNGG 1 cut(s) 552
AflIII ACRYGT 1 cut(s) 871
AgeI ACCGGT 1 cut(s) 1510
AgsI TTSAA 6 cut(s) 175, 255, 1021, 1196, 1292, 1538
AhlI ACTAGT 2 cut(s) 349, 1847
AjnI CCWGG 1 cut(s) 724
AjuI GAANNNNNNNTTGG 4 cut(s) 586, 618, 954, 986
AleI CACNNNNGTG 1 cut(s) 754
Alw21I GWGCWC 1 cut(s) 1855
Alw26I GTCTC 2 cut(s) 48, 629
AlwI GGATC 5 cut(s) 12, 179, 482, 1127, 1232
Aor51HI AGCGCT 2 cut(s) 315, 517
AoxI GGCC 9 cut(s) 831, 889, 926, 958, 1258, 1356, 1640, 1692, 1740
ApeKI GCWGC 6 cut(s) 23, 647, 776, 814, 977, 1469
ApoI RAATTY 2 cut(s) 1119, 1734
AsiGI ACCGGT 1 cut(s) 1510
Asp718I GGTACC 1 cut(s) 1718
AspLEI GCGC 2 cut(s) 316, 518
AspS9I GGNCC 1 cut(s) 1259
AsuC2I CCSGG 1 cut(s) 1485
AsuHPI GGTGA 7 cut(s) 47, 348, 383, 446, 519, 925, 1468
BaeI ACNNNNGTAYC 2 cut(s) 883, 916
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 1718
BanII GRGCYC 1 cut(s) 217
BbsI GAAGAC 2 cut(s) 1028, 1810
Bbv12I GWGCWC 1 cut(s) 1855
BbvI GCAGC 6 cut(s) 35, 659, 788, 801, 964, 1481
BccI CCATC 6 cut(s) 114, 465, 1058, 1100, 1496, 1719
BceAI ACGGC 1 cut(s) 425
BcgI CGANNNNNNTGC 2 cut(s) 12, 46
BciT130I CCWGG 1 cut(s) 726
BciVI GTATCC 1 cut(s) 415
BcnI CCSGG 1 cut(s) 1485
BcoDI GTCTC 2 cut(s) 48, 629
BcuI ACTAGT 2 cut(s) 349, 1847
BfaI CTAG 8 cut(s) 350, 539, 566, 845, 1230, 1347, 1385, 1848
BfmI CTRYAG 3 cut(s) 978, 1329, 1467
BfoI RGCGCY 2 cut(s) 317, 519
BfuI GTATCC 1 cut(s) 415
BlpI GCTNAGC 1 cut(s) 317
Bme1390I CCNGG 2 cut(s) 726, 1485
BmgT120I GGNCC 1 cut(s) 1259
BmiI GGNNCC 5 cut(s) 6, 216, 616, 1326, 1720
BmrFI CCNGG 2 cut(s) 726, 1485
BmsI GCATC 4 cut(s) 911, 1090, 1538, 1695
BoxI GACNNNNGTC 1 cut(s) 352
BpiI GAAGAC 2 cut(s) 1028, 1810
BpmI CTGGAG 1 cut(s) 816
Bpu1102I GCTNAGC 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 1485
Bsa29I ATCGAT 1 cut(s) 193
BsaBI GATNNNNATC 2 cut(s) 189, 1425
BsaHI GRCGYC 1 cut(s) 1025
BsaI GGTCTC 1 cut(s) 629
BsaJI CCNNGG 2 cut(s) 725, 1713
BsaWI WCCGGW 2 cut(s) 444, 1510
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bsc4I CCNNNNNNNGG 1 cut(s) 552
Bse118I RCCGGY 3 cut(s) 924, 1354, 1510
Bse1I ACTGG 3 cut(s) 557, 586, 897
Bse3DI GCAATG 1 cut(s) 1359
Bse8I GATNNNNATC 2 cut(s) 189, 1425
BseBI CCWGG 1 cut(s) 726
BseCI ATCGAT 1 cut(s) 193
BseDI CCNNGG 2 cut(s) 725, 1713
BseGI GGATG 3 cut(s) 288, 735, 1833
BseJI GATNNNNATC 2 cut(s) 189, 1425
BseLI CCNNNNNNNGG 1 cut(s) 552
BseMI GCAATG 1 cut(s) 1359
BseNI ACTGG 3 cut(s) 557, 586, 897
BseRI GAGGAG 2 cut(s) 92, 923
BseXI GCAGC 6 cut(s) 35, 659, 788, 801, 964, 1481
BsgI GTGCAG 1 cut(s) 227
Bsh1236I CGCG 1 cut(s) 878
Bsh1285I CGRYCG 1 cut(s) 381
BshFI GGCC 9 cut(s) 833, 891, 928, 960, 1260, 1358, 1642, 1694, 1742
BshNI GGYRCC 1 cut(s) 1718
BshTI ACCGGT 1 cut(s) 1510
BshVI ATCGAT 1 cut(s) 193
BsiEI CGRYCG 1 cut(s) 381
BsiHKAI GWGCWC 1 cut(s) 1855
BsiSI CCGG 5 cut(s) 445, 925, 1355, 1485, 1511
BsiWI CGTACG 1 cut(s) 653
BslI CCNNNNNNNGG 1 cut(s) 552
BsmAI GTCTC 2 cut(s) 48, 629
BsnI GGCC 9 cut(s) 833, 891, 928, 960, 1260, 1358, 1642, 1694, 1742
Bso31I GGTCTC 1 cut(s) 629
Bsp1286I GDGCHC 2 cut(s) 217, 1855
Bsp1407I TGTACA 1 cut(s) 1833
Bsp1720I GCTNAGC 1 cut(s) 317
BspACI CCGC 6 cut(s) 147, 644, 878, 1055, 1382, 1639
BspANI GGCC 9 cut(s) 833, 891, 928, 960, 1260, 1358, 1642, 1694, 1742
BspDI ATCGAT 1 cut(s) 193
BspFNI CGCG 1 cut(s) 878
BspHI TCATGA 1 cut(s) 1201
BspLI GGNNCC 5 cut(s) 6, 216, 616, 1326, 1720
BspMAI CTGCAG 2 cut(s) 982, 1471
BspPI GGATC 5 cut(s) 12, 179, 482, 1127, 1232
BspT107I GGYRCC 1 cut(s) 1718
BspTNI GGTCTC 1 cut(s) 629
BsrDI GCAATG 1 cut(s) 1359
BsrFI RCCGGY 3 cut(s) 924, 1354, 1510
BsrGI TGTACA 1 cut(s) 1833
BsrI ACTGG 3 cut(s) 557, 586, 897
BssAI RCCGGY 3 cut(s) 924, 1354, 1510
BssECI CCNNGG 2 cut(s) 725, 1713
BssNI GRCGYC 1 cut(s) 1025
BssT1I CCWWGG 1 cut(s) 1713
Bst2UI CCWGG 1 cut(s) 726
Bst4CI ACNGT 5 cut(s) 68, 207, 827, 967, 1456
Bst6I CTCTTC 1 cut(s) 1395
BstACI GRCGYC 1 cut(s) 1025
BstAUI TGTACA 1 cut(s) 1833
BstC8I GCNNGC 4 cut(s) 819, 889, 926, 1280
BstDEI CTNAG 1 cut(s) 317
BstEII GGTNACC 2 cut(s) 371, 452
BstF5I GGATG 3 cut(s) 288, 735, 1833
BstFNI CGCG 1 cut(s) 878
BstH2I RGCGCY 2 cut(s) 317, 519
BstHHI GCGC 2 cut(s) 316, 518
BstMAI GTCTC 2 cut(s) 48, 629
BstMCI CGRYCG 1 cut(s) 381
BstMWI GCNNNNNNNGC 5 cut(s) 305, 884, 1616, 1700, 1764
BstNI CCWGG 1 cut(s) 726
BstNSI RCATGY 4 cut(s) 437, 641, 875, 1282
BstPAI GACNNNNGTC 1 cut(s) 352
BstPI GGTNACC 2 cut(s) 371, 452
BstSCI CCNGG 2 cut(s) 724, 1483
BstSFI CTRYAG 3 cut(s) 978, 1329, 1467
BstUI CGCG 1 cut(s) 878
BstV1I GCAGC 6 cut(s) 35, 659, 788, 801, 964, 1481
BstV2I GAAGAC 2 cut(s) 1028, 1810
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 904
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 1 cut(s) 193
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 9 cut(s) 833, 891, 928, 960, 1260, 1358, 1642, 1694, 1742
BsuTUI ATCGAT 1 cut(s) 193
BtgZI GCGATG 1 cut(s) 1718
BtsCI GGATG 3 cut(s) 288, 735, 1833
BtsI GCAGTG 3 cut(s) 239, 807, 1464
BtsIMutI CAGTG 5 cut(s) 239, 550, 593, 807, 1464
Cac8I GCNNGC 4 cut(s) 819, 889, 926, 1280
CciI TCATGA 1 cut(s) 1201
CfoI GCGC 2 cut(s) 316, 518
Cfr10I RCCGGY 3 cut(s) 924, 1354, 1510
Cfr13I GGNCC 1 cut(s) 1259
ClaI ATCGAT 1 cut(s) 193
CseI GACGC 3 cut(s) 1033, 1067, 1450
Csp6I GTAC 6 cut(s) 654, 660, 1012, 1719, 1786, 1834
CspAI ACCGGT 1 cut(s) 1510
CspCI CAANNNNNGTGG 2 cut(s) 1566, 1601
CviQI GTAC 6 cut(s) 654, 660, 1012, 1719, 1786, 1834
DdeI CTNAG 1 cut(s) 317
EaeI YGGCCR 2 cut(s) 1356, 1640
Eam1104I CTCTTC 1 cut(s) 1395
EarI CTCTTC 1 cut(s) 1395
EciI GGCGGA 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 1713
Eco24I GRGCYC 1 cut(s) 217
Eco31I GGTCTC 1 cut(s) 629
Eco47III AGCGCT 2 cut(s) 315, 517
Eco91I GGTNACC 2 cut(s) 371, 452
EcoO65I GGTNACC 2 cut(s) 371, 452
EcoRII CCWGG 1 cut(s) 724
EcoT14I CCWWGG 1 cut(s) 1713
EcoT22I ATGCAT 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 1713
FalI AAGNNNNNCTT 4 cut(s) 453, 485, 1095, 1127
FauNDI CATATG 1 cut(s) 1763
FokI GGATG 3 cut(s) 275, 742, 1840
FriOI GRGCYC 1 cut(s) 217
FspBI CTAG 8 cut(s) 350, 539, 566, 845, 1230, 1347, 1385, 1848
GlaI GCGC 2 cut(s) 315, 517
GsuI CTGGAG 1 cut(s) 816
HaeII RGCGCY 2 cut(s) 317, 519
HaeIII GGCC 9 cut(s) 833, 891, 928, 960, 1260, 1358, 1642, 1694, 1742
HapII CCGG 5 cut(s) 445, 925, 1355, 1485, 1511
HgaI GACGC 3 cut(s) 1033, 1067, 1450
HhaI GCGC 2 cut(s) 316, 518
Hin1I GRCGYC 1 cut(s) 1025
Hin6I GCGC 2 cut(s) 314, 516
HinP1I GCGC 2 cut(s) 314, 516
HincII GTYRAC 2 cut(s) 203, 745
HindII GTYRAC 2 cut(s) 203, 745
HinfI GANTC 8 cut(s) 152, 482, 524, 760, 916, 1041, 1426, 1556
HpaII CCGG 5 cut(s) 445, 925, 1355, 1485, 1511
HphI GGTGA 7 cut(s) 47, 348, 383, 446, 519, 925, 1468
Hpy166II GTNNAC 8 cut(s) 203, 347, 745, 759, 992, 1144, 1250, 1352
Hpy188I TCNGA 5 cut(s) 111, 383, 401, 487, 1158
Hpy188III TCNNGA 9 cut(s) 188, 255, 795, 845, 855, 1115, 1196, 1202, 1292
Hpy8I GTNNAC 8 cut(s) 203, 347, 745, 759, 992, 1144, 1250, 1352
Hpy99I CGWCG 1 cut(s) 1444
HpyAV CCTTC 6 cut(s) 220, 320, 612, 1313, 1330, 1576
HpyCH4III ACNGT 5 cut(s) 68, 207, 827, 967, 1456
HpyCH4IV ACGT 1 cut(s) 246
HpyF10VI GCNNNNNNNGC 5 cut(s) 305, 884, 1616, 1700, 1764
HpyF3I CTNAG 1 cut(s) 317
HpySE526I ACGT 1 cut(s) 246
Hsp92I GRCGYC 1 cut(s) 1025
HspAI GCGC 2 cut(s) 314, 516
KpnI GGTACC 1 cut(s) 1722
KroI GCCGGC 1 cut(s) 924
KroNI GCCGGC 1 cut(s) 926
LmnI GCTCC 4 cut(s) 220, 329, 402, 797
Lsp1109I GCAGC 6 cut(s) 35, 659, 788, 801, 964, 1481
LweI GCATC 4 cut(s) 911, 1090, 1538, 1695
MaeI CTAG 8 cut(s) 350, 539, 566, 845, 1230, 1347, 1385, 1848
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 7 cut(s) 230, 354, 371, 452, 525, 1456, 1747
MfeI CAATTG 2 cut(s) 90, 494
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 217, 1855
MlyI GAGTC 4 cut(s) 533, 754, 1035, 1550
MmeI TCCRAC 1 cut(s) 617
Mph1103I ATGCAT 1 cut(s) 435
MroNI GCCGGC 1 cut(s) 924
MseI TTAA 3 cut(s) 476, 1601, 1649
MslI CAYNNNNRTG 4 cut(s) 754, 1277, 1319, 1781
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 5 cut(s) 445, 925, 1355, 1485, 1511
MspR9I CCNGG 2 cut(s) 726, 1485
MunI CAATTG 2 cut(s) 90, 494
MvaI CCWGG 1 cut(s) 726
MvnI CGCG 1 cut(s) 878
MwoI GCNNNNNNNGC 5 cut(s) 305, 884, 1616, 1700, 1764
NaeI GCCGGC 1 cut(s) 926
NciI CCSGG 1 cut(s) 1485
NdeI CATATG 1 cut(s) 1763
NgoMIV GCCGGC 1 cut(s) 924
NlaIV GGNNCC 5 cut(s) 6, 216, 616, 1326, 1720
NmuCI GTSAC 5 cut(s) 354, 371, 452, 525, 1456
NsiI ATGCAT 1 cut(s) 435
NspI RCATGY 4 cut(s) 437, 641, 875, 1282
OliI CACNNNNGTG 1 cut(s) 754
PaeI GCATGC 1 cut(s) 1282
PagI TCATGA 1 cut(s) 1201
PciI ACATGT 1 cut(s) 871
PdiI GCCGGC 1 cut(s) 926
PfeI GAWTC 4 cut(s) 152, 482, 916, 1426
Pfl23II CGTACG 1 cut(s) 653
PinAI ACCGGT 1 cut(s) 1510
Ple19I CGATCG 1 cut(s) 381
PleI GAGTC 4 cut(s) 532, 754, 1035, 1550
PpsI GAGTC 4 cut(s) 532, 754, 1035, 1550
PscI ACATGT 1 cut(s) 871
PshAI GACNNNNGTC 1 cut(s) 352
PsiI TTATAA 1 cut(s) 1820
Psp6I CCWGG 1 cut(s) 724
PspEI GGTNACC 2 cut(s) 371, 452
PspGI CCWGG 1 cut(s) 724
PspLI CGTACG 1 cut(s) 653
PspN4I GGNNCC 5 cut(s) 6, 216, 616, 1326, 1720
PspPI GGNCC 1 cut(s) 1259
PstI CTGCAG 2 cut(s) 982, 1471
PsuI RGATCY 1 cut(s) 4
PvuI CGATCG 1 cut(s) 381
PvuII CAGCTG 1 cut(s) 362
RsaI GTAC 6 cut(s) 655, 661, 1013, 1720, 1787, 1835
RsaNI GTAC 6 cut(s) 654, 660, 1012, 1719, 1786, 1834
RseI CAYNNNNRTG 4 cut(s) 754, 1277, 1319, 1781
SaqAI TTAA 3 cut(s) 476, 1601, 1649
Sau96I GGNCC 1 cut(s) 1259
SchI GAGTC 4 cut(s) 533, 754, 1035, 1550
ScrFI CCNGG 2 cut(s) 726, 1485
SduI GDGCHC 2 cut(s) 217, 1855
SfaNI GCATC 4 cut(s) 911, 1090, 1538, 1695
SfcI CTRYAG 3 cut(s) 978, 1329, 1467
SmiMI CAYNNNNRTG 4 cut(s) 754, 1277, 1319, 1781
SpeI ACTAGT 2 cut(s) 349, 1847
SphI GCATGC 1 cut(s) 1282
SsiI CCGC 6 cut(s) 147, 644, 878, 1055, 1382, 1639
SspI AATATT 2 cut(s) 810, 1581
SspMI CTAG 8 cut(s) 350, 539, 566, 845, 1230, 1347, 1385, 1848
StyD4I CCNGG 2 cut(s) 724, 1483
StyI CCWWGG 1 cut(s) 1713
TaaI ACNGT 5 cut(s) 68, 207, 827, 967, 1456
TaiI ACGT 1 cut(s) 249
TatI WGTACW 3 cut(s) 1011, 1785, 1833
TauI GCSGC 4 cut(s) 647, 881, 1384, 1642
TfiI GAWTC 4 cut(s) 152, 482, 916, 1426
Tru1I TTAA 3 cut(s) 476, 1601, 1649
Tru9I TTAA 3 cut(s) 476, 1601, 1649
TscAI CASTG 5 cut(s) 246, 557, 593, 807, 1471
TseFI GTSAC 5 cut(s) 354, 371, 452, 525, 1456
TseI GCWGC 6 cut(s) 23, 647, 776, 814, 977, 1469
Tsp45I GTSAC 5 cut(s) 354, 371, 452, 525, 1456
TspRI CASTG 5 cut(s) 246, 557, 593, 807, 1471
XapI RAATTY 2 cut(s) 1119, 1734
XbaI TCTAGA 1 cut(s) 844
XceI RCATGY 4 cut(s) 437, 641, 875, 1282
XspI CTAG 8 cut(s) 350, 539, 566, 845, 1230, 1347, 1385, 1848
Zsp2I ATGCAT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.