FvH4_4g08290

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000687
Physical Location & Seq
Forward (+)
8183083 .. 8185656
2574 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08290.t1

Sequence Viewer

Length: 546 bp
ATGGAGAAACTCCAATTTCGGGTGACCTTCCTTGGAGGGGACACGATTGTCAACAAGTGGATGAGTTTTGCTCTAGAGAAAAATGTGAAAGAGTTGGCTATCAGCTTTAAACAACGTCCAATGCATGCAGTTAAGGCTGATTTCAGGACGAGGAATGTTTCAAACCAAGTTATTGCTCCTTGTGCTTCCTTGGCGGCTTGTTCCGCGGCCACGACCACCGCCGCCATGGAACGTCATACACCTAGAAGTTCGACCCTCACTTTGTTGAAAATTTTCTGCTGCAATATTACGATCATAGTGGAAGCACAGAAACTTGAAGCTTTCAGACTCATTGGTAGCCGTTCCCAAGTCATGCAGAAGATGAGCAATGGGAACTTCAACATTGTCGCTTGTCGAAAATTGAAGAAGTTGGAGTTCGTTAGTAGAAATCTGCTGCGTTTTGAAGGCTCTACGGTTTCCAGAAGGTGTTATCTGCTCCCCACCGGCCACCACTGCCCCGGGTACGGCTTCTTGATGTGGATCGGAGATTTCTGCTCCTGCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

20.38

Weight (kDa)

9.68

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 47
AccII CGCG 1 cut(s) 206
AciI CCGC 5 cut(s) 194, 204, 206, 219, 222
AclWI GGATC 1 cut(s) 527
AcoI YGGCCR 2 cut(s) 207, 484
AcsI RAATTY 1 cut(s) 270
AfaI GTAC 1 cut(s) 503
AfiI CCNNNNNNNGG 3 cut(s) 19, 37, 503
AgsI TTSAA 6 cut(s) 162, 268, 317, 379, 403, 443
AluBI AGCT 2 cut(s) 105, 320
AluI AGCT 2 cut(s) 105, 320
AlwI GGATC 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 497
AoxI GGCC 2 cut(s) 207, 484
ApeKI GCWGC 2 cut(s) 279, 433
ApoI RAATTY 1 cut(s) 270
ArsI GACNNNNNNTTYG 2 cut(s) 244, 276
Asp700I GAANNNNTTC 1 cut(s) 272
AsuC2I CCSGG 2 cut(s) 498, 499
AsuHPI GGTGA 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 497
BbvI GCAGC 2 cut(s) 266, 420
BceAI ACGGC 2 cut(s) 324, 520
BcnI CCSGG 2 cut(s) 498, 499
BfaI CTAG 2 cut(s) 74, 243
BisI GCNGC 5 cut(s) 195, 207, 222, 280, 434
BlsI GCNGC 5 cut(s) 196, 208, 223, 281, 435
Bme1390I CCNGG 2 cut(s) 498, 499
BmeT110I CYCGRG 1 cut(s) 497
BmrFI CCNGG 2 cut(s) 498, 499
BplI GAGNNNNNCTC 2 cut(s) 55, 87
BpuMI CCSGG 2 cut(s) 498, 499
BsaBI GATNNNNATC 1 cut(s) 518
BsaJI CCNNGG 6 cut(s) 31, 189, 204, 225, 496, 497
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 37, 503
Bse118I RCCGGY 1 cut(s) 482
Bse3DI GCAATG 1 cut(s) 373
Bse8I GATNNNNATC 1 cut(s) 518
BseDI CCNNGG 6 cut(s) 31, 189, 204, 225, 496, 497
BseGI GGATG 1 cut(s) 66
BseJI GATNNNNATC 1 cut(s) 518
BseLI CCNNNNNNNGG 3 cut(s) 19, 37, 503
BseMI GCAATG 1 cut(s) 373
BseXI GCAGC 2 cut(s) 266, 420
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 2 cut(s) 209, 486
BsiHKCI CYCGRG 1 cut(s) 497
BsiSI CCGG 2 cut(s) 483, 498
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 3 cut(s) 19, 37, 503
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 2 cut(s) 209, 486
BsoBI CYCGRG 1 cut(s) 497
Bsp143I GATC 2 cut(s) 291, 519
Bsp19I CCATGG 1 cut(s) 225
BspACI CCGC 5 cut(s) 194, 204, 206, 219, 222
BspANI GGCC 2 cut(s) 209, 486
BspFNI CGCG 1 cut(s) 206
BspPI GGATC 1 cut(s) 527
BsrDI GCAATG 1 cut(s) 373
BsrFI RCCGGY 1 cut(s) 482
BssAI RCCGGY 1 cut(s) 482
BssECI CCNNGG 6 cut(s) 31, 189, 204, 225, 496, 497
BssMI GATC 2 cut(s) 291, 519
BssT1I CCWWGG 3 cut(s) 31, 189, 225
Bst4CI ACNGT 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 126
BstDSI CCRYGG 2 cut(s) 204, 225
BstEII GGTNACC 1 cut(s) 22
BstF5I GGATG 1 cut(s) 66
BstFNI CGCG 1 cut(s) 206
BstKTI GATC 2 cut(s) 294, 522
BstMBI GATC 2 cut(s) 291, 519
BstMWI GCNNNNNNNGC 5 cut(s) 134, 182, 191, 203, 492
BstNSI RCATGY 1 cut(s) 128
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 496, 497
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 2 cut(s) 266, 420
BsuRI GGCC 2 cut(s) 209, 486
BtgI CCRYGG 2 cut(s) 204, 225
BtsCI GGATG 1 cut(s) 66
BtsI GCAGTG 1 cut(s) 490
BtsIMutI CAGTG 1 cut(s) 490
Cac8I GCNNGC 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 482
Cfr42I CCGCGG 1 cut(s) 207
Cfr9I CCCGGG 1 cut(s) 497
Csp6I GTAC 1 cut(s) 502
CviAII CATG 3 cut(s) 125, 226, 352
CviQI GTAC 1 cut(s) 502
DpnI GATC 2 cut(s) 293, 521
DpnII GATC 2 cut(s) 291, 519
DraI TTTAAA 1 cut(s) 109
DrdI GACNNNNNNGTC 1 cut(s) 47
DseDI GACNNNNNNGTC 1 cut(s) 47
EaeI YGGCCR 2 cut(s) 207, 484
Eco130I CCWWGG 3 cut(s) 31, 189, 225
Eco88I CYCGRG 1 cut(s) 497
Eco91I GGTNACC 1 cut(s) 22
EcoO65I GGTNACC 1 cut(s) 22
EcoT14I CCWWGG 3 cut(s) 31, 189, 225
EcoT22I ATGCAT 1 cut(s) 126
ErhI CCWWGG 3 cut(s) 31, 189, 225
FaeI CATG 3 cut(s) 128, 229, 355
FaiI YATR 5 cut(s) 126, 227, 237, 296, 353
FaqI GGGAC 1 cut(s) 53
FatI CATG 3 cut(s) 124, 225, 351
Fnu4HI GCNGC 5 cut(s) 195, 207, 222, 280, 434
FokI GGATG 1 cut(s) 73
Fsp4HI GCNGC 5 cut(s) 195, 207, 222, 280, 434
FspBI CTAG 2 cut(s) 74, 243
GluI GCNGC 5 cut(s) 195, 207, 222, 280, 434
HaeIII GGCC 2 cut(s) 209, 486
HapII CCGG 2 cut(s) 483, 498
Hin1II CATG 3 cut(s) 128, 229, 355
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 318
HinfI GANTC 1 cut(s) 327
HpaII CCGG 2 cut(s) 483, 498
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 2 cut(s) 326, 524
Hpy188III TCNNGA 4 cut(s) 74, 145, 459, 511
Hpy8I GTNNAC 1 cut(s) 52
HpyAV CCTTC 3 cut(s) 37, 437, 456
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4IV ACGT 2 cut(s) 115, 232
HpyCH4V TGCA 4 cut(s) 124, 128, 282, 355
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 182, 191, 203, 492
HpySE526I ACGT 2 cut(s) 115, 232
Hsp92II CATG 3 cut(s) 128, 229, 355
KspI CCGCGG 1 cut(s) 207
Kzo9I GATC 2 cut(s) 291, 519
LmnI GCTCC 3 cut(s) 181, 480, 539
LpnPI CCDG 4 cut(s) 130, 472, 496, 511
Lsp1109I GCAGC 2 cut(s) 266, 420
MaeI CTAG 2 cut(s) 74, 243
MaeII ACGT 2 cut(s) 115, 232
MaeIII GTNAC 1 cut(s) 22
MalI GATC 2 cut(s) 293, 521
MboI GATC 2 cut(s) 291, 519
MboII GAAGA 2 cut(s) 370, 415
MluCI AATT 3 cut(s) 14, 270, 398
MlyI GAGTC 1 cut(s) 321
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 3 cut(s) 29, 144, 266
Mph1103I ATGCAT 1 cut(s) 126
MroXI GAANNNNTTC 1 cut(s) 272
MseI TTAA 2 cut(s) 108, 132
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 2 cut(s) 483, 498
MspR9I CCNGG 2 cut(s) 498, 499
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 5 cut(s) 134, 182, 191, 203, 492
NciI CCSGG 2 cut(s) 498, 499
NcoI CCATGG 1 cut(s) 225
NdeII GATC 2 cut(s) 291, 519
NlaIII CATG 3 cut(s) 128, 229, 355
NmuCI GTSAC 1 cut(s) 22
NsiI ATGCAT 1 cut(s) 126
NspI RCATGY 1 cut(s) 128
PaeI GCATGC 1 cut(s) 128
PdmI GAANNNNTTC 1 cut(s) 272
PkrI GCNGC 5 cut(s) 196, 208, 223, 281, 435
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
PspEI GGTNACC 1 cut(s) 22
RsaI GTAC 1 cut(s) 503
RsaNI GTAC 1 cut(s) 502
SacII CCGCGG 1 cut(s) 207
SaqAI TTAA 2 cut(s) 108, 132
SatI GCNGC 5 cut(s) 195, 207, 222, 280, 434
Sau3AI GATC 2 cut(s) 291, 519
SchI GAGTC 1 cut(s) 321
ScrFI CCNGG 2 cut(s) 498, 499
SetI ASST 7 cut(s) 29, 107, 118, 235, 244, 322, 467
Sfr303I CCGCGG 1 cut(s) 207
SgrBI CCGCGG 1 cut(s) 207
SmaI CCCGGG 1 cut(s) 499
SphI GCATGC 1 cut(s) 128
Sse9I AATT 3 cut(s) 14, 270, 398
SsiI CCGC 5 cut(s) 194, 204, 206, 219, 222
SspI AATATT 1 cut(s) 286
SspMI CTAG 2 cut(s) 74, 243
StyD4I CCNGG 2 cut(s) 496, 497
StyI CCWWGG 3 cut(s) 31, 189, 225
TaaI ACNGT 1 cut(s) 454
TaiI ACGT 2 cut(s) 118, 235
TaqI TCGA 2 cut(s) 251, 394
TasI AATT 3 cut(s) 14, 270, 398
TauI GCSGC 3 cut(s) 197, 209, 224
Tru1I TTAA 2 cut(s) 108, 132
Tru9I TTAA 2 cut(s) 108, 132
TscAI CASTG 1 cut(s) 497
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 2 cut(s) 279, 433
Tsp45I GTSAC 1 cut(s) 22
TspMI CCCGGG 1 cut(s) 497
TspRI CASTG 1 cut(s) 497
XapI RAATTY 1 cut(s) 270
XbaI TCTAGA 1 cut(s) 73
XceI RCATGY 1 cut(s) 128
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XmaI CCCGGG 1 cut(s) 497
XmnI GAANNNNTTC 1 cut(s) 272
XspI CTAG 2 cut(s) 74, 243
Zsp2I ATGCAT 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.