Rw2G046290

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000687
Physical Location & Seq
Reverse (-)
73591949 .. 73603592
11644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G046290.1

Sequence Viewer

Length: 1386 bp
ATGGCTAAGAAGAGAAAACTATCAGGCAGCTGCAACATGGCAAACAGAAATAAGAGGATCAAAAAGGAGGAGGAGGTTCAAAGCCAAAACACCGGGGGTAGTATGGTGGTGGACACATACACATCCCAATTACCCGAACTTGTCATCAACCAAATCTTCTCTCATCTTTCCACCAAGTCCGCCATTAGGGCAAGCATTCTTTCTAAGGAATGGGCAAGCGTGTGTTCTTCGGTTCTCATTTTAGATATCGATGAGGATGAAGGGAAGAAGCAAATTGCTGATCACAGGGATCGACGTAGAAAGTTCCTTAAGTTCTTGAGCAATTGTTTGAAGAGCCGTGAGAAAGAAAAGTACCTGGAGAAGTTCAGGCTCCGTATGAGTTATTTTGGAGGTGACTGTGCCATAAATAAGTCATTGAGTTTTGCTTTGGAAAGAAACGTTAAAGAGTTGGATATTTGCTTCAAAAGAGAGAACGAGGTCAGAAAAGGCAGATACTATTGCTTACAGCAGACAGCTCTCAATAATGCAAAATCTTTAACCATTTTGAAGTTGGAGTATTTGAAAATAGAAGAGGACAGCATCGACACTATAAGCCTTACGTCTTTGAAAACTTTGTCTCTAAAAGCTGTGAAATTCATGACTAACACTGGATTCTCACGCTTATATTCGGGGTGTCCTTCCATCGAGTATTTGTCATTAACTTCGTGTCACGGCTGGGATGTTAGACTTTCAAGTTCGGGGCTCAAACACTTGGAAATTTTGTGTTGCGAAATCAAACTTAAAGTCGAAGCAACAAACCTTGAATCCTTTAAATTCCGTGGTGACAATGGACTTCGTCATCATATCATTGACATGCGCAACTTCTACATTGCCTCTTGTCAAACGCTCAGAAGTCTTGAGTTCTCTCATGCACACTTTGCAATTGGATGGTTCAAAGGCCTCGATTCGAGATTTCCTCTTCTTGAGAGGTTGGTTTTACATACATGCTTCAGCTACTCGGGGATTATCAACATCTTTAGCCAGCATCTGAAAAGCTTTGTGTTTCATGAATGCGGAAACATTTTGCCTAATTTTTCTATGACTGCTCCAAATTTATCGGAGGCTACAATCACAATTGAAGAGAGAAGAGACTATGATCGCCTGAGATATTTTCTTGGAAATTTTGATTGCTCTAAGAAAATACGTCTACATGTTAATGAGACTGAGCTATGTACTTTTCCAGAACGGATAAGAAACGGATTCACCCCACCATTGCCTAGTCTCAAGCATCTTGATGTAGAGATCGGTACTTCTACAACTGAAGTGACACATTCTGCCTTAATGGACTCCTTGAATTGGATGGCGCCAGCGTCTCGAGTTTCGATCCATAAAACAACATTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

461

Amino Acids

52.92

Weight (kDa)

9.11

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 42 - 79 4.6e-08 F-box domain
LRR_At5g56370 PF24758 143 - 276 1.4e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 857
AccB1I GGYRCC 1 cut(s) 1342
AccI GTMKAC 1 cut(s) 1186
AciI CCGC 2 cut(s) 180, 1053
AclI AACGTT 1 cut(s) 438
AclWI GGATC 3 cut(s) 65, 297, 1357
AcsI RAATTY 5 cut(s) 632, 756, 812, 1090, 1159
AcuI CTGAAG 2 cut(s) 973, 1320
AcyI GRCGYC 1 cut(s) 1343
AfaI GTAC 3 cut(s) 353, 1213, 1288
AfiI CCNNNNNNNGG 2 cut(s) 186, 1335
AflII CTTAAG 1 cut(s) 308
AflIII ACRYGT 1 cut(s) 1189
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 6 cut(s) 30, 515, 626, 993, 1035, 1207
AluI AGCT 6 cut(s) 30, 515, 626, 993, 1035, 1207
Alw26I GTCTC 5 cut(s) 621, 1122, 1193, 1265, 1356
AlwI GGATC 3 cut(s) 65, 297, 1357
AlwNI CAGNNNCTG 1 cut(s) 1027
Ama87I CYCGRG 2 cut(s) 997, 1353
AoxI GGCC 1 cut(s) 937
ApeKI GCWGC 2 cut(s) 27, 30
ApoI RAATTY 5 cut(s) 632, 756, 812, 1090, 1159
ArsI GACNNNNNNTTYG 2 cut(s) 768, 800
AspLEI GCGC 2 cut(s) 858, 1345
AsuC2I CCSGG 1 cut(s) 94
AsuHPI GGTGA 3 cut(s) 404, 833, 1234
AvaI CYCGRG 2 cut(s) 997, 1353
BanI GGYRCC 1 cut(s) 1342
BanII GRGCYC 1 cut(s) 744
BbvI GCAGC 2 cut(s) 17, 39
BccI CCATC 3 cut(s) 689, 921, 1333
BceAI ACGGC 2 cut(s) 321, 727
BciT130I CCWGG 1 cut(s) 356
BclI TGATCA 1 cut(s) 280
BcnI CCSGG 1 cut(s) 94
BcoDI GTCTC 5 cut(s) 621, 1122, 1193, 1265, 1356
BfaI CTAG 1 cut(s) 1257
BfoI RGCGCY 1 cut(s) 1346
BfrI CTTAAG 1 cut(s) 308
BglI GCCNNNNNGGC 1 cut(s) 188
BisI GCNGC 2 cut(s) 28, 31
BlsI GCNGC 2 cut(s) 29, 32
Bme1390I CCNGG 2 cut(s) 94, 356
BmeT110I CYCGRG 2 cut(s) 997, 1353
BmiI GGNNCC 2 cut(s) 371, 1344
BmrFI CCNGG 2 cut(s) 94, 356
BmsI GCATC 3 cut(s) 588, 1033, 1276
BplI GAGNNNNNCTC 2 cut(s) 940, 972
BpmI CTGGAG 1 cut(s) 377
BpuEI CTTGAG 4 cut(s) 337, 917, 983, 1247
BpuMI CCSGG 1 cut(s) 94
Bsa29I ATCGAT 1 cut(s) 249
BsaHI GRCGYC 1 cut(s) 1343
BsaJI CCNNGG 2 cut(s) 93, 817
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 1335
Bse1I ACTGG 1 cut(s) 652
Bse3DI GCAATG 2 cut(s) 867, 1250
BseBI CCWGG 1 cut(s) 356
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 2 cut(s) 93, 817
BseGI GGATG 5 cut(s) 122, 262, 724, 932, 1344
BseLI CCNNNNNNNGG 2 cut(s) 186, 1335
BseMI GCAATG 2 cut(s) 867, 1250
BseMII CTCAG 3 cut(s) 901, 1133, 1194
BseNI ACTGG 1 cut(s) 652
BseRI GAGGAG 2 cut(s) 83, 86
BseXI GCAGC 2 cut(s) 17, 39
BseYI CCCAGC 1 cut(s) 714
BshFI GGCC 1 cut(s) 939
BshNI GGYRCC 1 cut(s) 1342
BshVI ATCGAT 1 cut(s) 249
BsiHKCI CYCGRG 2 cut(s) 997, 1353
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 2 cut(s) 186, 1335
BsmAI GTCTC 5 cut(s) 621, 1122, 1193, 1265, 1356
BsmBI CGTCTC 1 cut(s) 1356
BsmI GAATGC 2 cut(s) 195, 1055
BsnI GGCC 1 cut(s) 939
BsoBI CYCGRG 2 cut(s) 997, 1353
Bsp1286I GDGCHC 1 cut(s) 744
Bsp143I GATC 6 cut(s) 57, 280, 289, 1135, 1281, 1362
BspACI CCGC 2 cut(s) 180, 1053
BspANI GGCC 1 cut(s) 939
BspCNI CTCAG 3 cut(s) 900, 1134, 1195
BspDI ATCGAT 1 cut(s) 249
BspHI TCATGA 2 cut(s) 636, 1045
BspLI GGNNCC 2 cut(s) 371, 1344
BspPI GGATC 3 cut(s) 65, 297, 1357
BspQI GCTCTTC 1 cut(s) 326
BspT107I GGYRCC 1 cut(s) 1342
BspTI CTTAAG 1 cut(s) 308
BsrDI GCAATG 2 cut(s) 867, 1250
BsrI ACTGG 1 cut(s) 652
BssECI CCNNGG 2 cut(s) 93, 817
BssMI GATC 6 cut(s) 57, 280, 289, 1135, 1281, 1362
BssNI GRCGYC 1 cut(s) 1343
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 1 cut(s) 398
Bst6I CTCTTC 6 cut(s) 5, 326, 564, 963, 1113, 1120
BstACI GRCGYC 1 cut(s) 1343
BstAFI CTTAAG 1 cut(s) 308
BstAPI GCANNNNNTGC 1 cut(s) 917
BstC8I GCNNGC 4 cut(s) 193, 217, 1022, 1347
BstDEI CTNAG 7 cut(s) 6, 204, 887, 1142, 1173, 1203, 1383
BstDSI CCRYGG 1 cut(s) 817
BstF5I GGATG 5 cut(s) 122, 262, 724, 932, 1344
BstH2I RGCGCY 1 cut(s) 1346
BstHHI GCGC 2 cut(s) 858, 1345
BstKTI GATC 6 cut(s) 60, 283, 292, 1138, 1284, 1365
BstMAI GTCTC 5 cut(s) 621, 1122, 1193, 1265, 1356
BstMBI GATC 6 cut(s) 57, 280, 289, 1135, 1281, 1362
BstMWI GCNNNNNNNGC 2 cut(s) 188, 917
BstNI CCWGG 1 cut(s) 356
BstNSI RCATGY 3 cut(s) 856, 987, 1193
BstSCI CCNGG 2 cut(s) 92, 354
BstV1I GCAGC 2 cut(s) 17, 39
Bsu15I ATCGAT 1 cut(s) 249
BsuRI GGCC 1 cut(s) 939
BsuTUI ATCGAT 1 cut(s) 249
BtgI CCRYGG 1 cut(s) 817
BtsCI GGATG 5 cut(s) 122, 262, 724, 932, 1344
BtsIMutI CAGTG 1 cut(s) 645
Cac8I GCNNGC 4 cut(s) 193, 217, 1022, 1347
CaiI CAGNNNCTG 1 cut(s) 1027
CciI TCATGA 2 cut(s) 636, 1045
CfoI GCGC 2 cut(s) 858, 1345
ClaI ATCGAT 1 cut(s) 249
CseI GACGC 1 cut(s) 1338
Csp6I GTAC 3 cut(s) 352, 1212, 1287
CviAII CATG 7 cut(s) 37, 637, 853, 908, 984, 1046, 1190
CviQI GTAC 3 cut(s) 352, 1212, 1287
DdeI CTNAG 7 cut(s) 6, 204, 887, 1142, 1173, 1203, 1383
DinI GGCGCC 1 cut(s) 1344
DpnI GATC 6 cut(s) 59, 282, 291, 1137, 1283, 1364
DpnII GATC 6 cut(s) 57, 280, 289, 1135, 1281, 1362
DraI TTTAAA 1 cut(s) 811
Eam1104I CTCTTC 6 cut(s) 5, 326, 564, 963, 1113, 1120
EarI CTCTTC 6 cut(s) 5, 326, 564, 963, 1113, 1120
EciI GGCGGA 1 cut(s) 169
Eco147I AGGCCT 1 cut(s) 939
Eco24I GRGCYC 1 cut(s) 744
Eco32I GATATC 1 cut(s) 247
Eco57I CTGAAG 2 cut(s) 973, 1320
Eco88I CYCGRG 2 cut(s) 997, 1353
EcoRII CCWGG 1 cut(s) 354
EcoRV GATATC 1 cut(s) 247
EcoT38I GRGCYC 1 cut(s) 744
EgeI GGCGCC 1 cut(s) 1344
EheI GGCGCC 1 cut(s) 1344
Esp3I CGTCTC 1 cut(s) 1356
FaeI CATG 7 cut(s) 40, 640, 856, 911, 987, 1049, 1193
FatI CATG 7 cut(s) 36, 636, 852, 907, 983, 1045, 1189
FbaI TGATCA 1 cut(s) 280
FblI GTMKAC 1 cut(s) 1186
Fnu4HI GCNGC 2 cut(s) 28, 31
FokI GGATG 5 cut(s) 109, 269, 731, 939, 1351
FriOI GRGCYC 1 cut(s) 744
Fsp4HI GCNGC 2 cut(s) 28, 31
FspBI CTAG 1 cut(s) 1257
FspI TGCGCA 1 cut(s) 857
GlaI GCGC 2 cut(s) 857, 1344
GluI GCNGC 2 cut(s) 28, 31
GsaI CCCAGC 1 cut(s) 718
GsuI CTGGAG 1 cut(s) 377
HaeII RGCGCY 1 cut(s) 1346
HaeIII GGCC 1 cut(s) 939
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 1338
HhaI GCGC 2 cut(s) 858, 1345
Hin1I GRCGYC 1 cut(s) 1343
Hin1II CATG 7 cut(s) 40, 640, 856, 911, 987, 1049, 1193
Hin6I GCGC 2 cut(s) 856, 1343
HinP1I GCGC 2 cut(s) 856, 1343
HindIII AAGCTT 1 cut(s) 1033
HinfI GANTC 5 cut(s) 651, 803, 944, 1239, 1325
HpaII CCGG 1 cut(s) 93
HphI GGTGA 3 cut(s) 404, 833, 1234
Hpy166II GTNNAC 2 cut(s) 112, 1187
Hpy188I TCNGA 4 cut(s) 482, 890, 1029, 1099
Hpy188III TCNNGA 9 cut(s) 316, 637, 896, 948, 962, 1046, 1220, 1271, 1353
Hpy8I GTNNAC 2 cut(s) 112, 1187
Hpy99I CGWCG 1 cut(s) 297
HpyAV CCTTC 2 cut(s) 254, 687
HpyCH4III ACNGT 1 cut(s) 398
HpyCH4IV ACGT 4 cut(s) 295, 438, 599, 1183
HpyCH4V TGCA 4 cut(s) 33, 527, 911, 920
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 917
HpyF3I CTNAG 7 cut(s) 6, 204, 887, 1142, 1173, 1203, 1383
HpySE526I ACGT 4 cut(s) 295, 438, 599, 1183
Hsp92I GRCGYC 1 cut(s) 1343
Hsp92II CATG 7 cut(s) 40, 640, 856, 911, 987, 1049, 1193
HspAI GCGC 2 cut(s) 856, 1343
KasI GGCGCC 1 cut(s) 1342
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 6 cut(s) 57, 280, 289, 1135, 1281, 1362
LguI GCTCTTC 1 cut(s) 326
LmnI GCTCC 2 cut(s) 375, 1090
Lsp1109I GCAGC 2 cut(s) 17, 39
LweI GCATC 3 cut(s) 588, 1033, 1276
MaeI CTAG 1 cut(s) 1257
MaeII ACGT 4 cut(s) 295, 438, 599, 1183
MaeIII GTNAC 4 cut(s) 392, 707, 821, 1303
MalI GATC 6 cut(s) 59, 282, 291, 1137, 1283, 1364
MboI GATC 6 cut(s) 57, 280, 289, 1135, 1281, 1362
MboII GAAGA 9 cut(s) 22, 148, 219, 277, 343, 581, 950, 1130, 1137
MfeI CAATTG 3 cut(s) 322, 921, 1113
MhlI GDGCHC 1 cut(s) 744
Mly113I GGCGCC 1 cut(s) 1343
MlyI GAGTC 1 cut(s) 1319
MmeI TCCRAC 2 cut(s) 429, 531
MseI TTAA 8 cut(s) 309, 441, 536, 698, 780, 810, 1194, 1319
MslI CAYNNNNRTG 3 cut(s) 851, 1194, 1272
MspA1I CMGCKG 1 cut(s) 30
MspCI CTTAAG 1 cut(s) 308
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 2 cut(s) 94, 356
MunI CAATTG 3 cut(s) 322, 921, 1113
Mva1269I GAATGC 2 cut(s) 195, 1055
MvaI CCWGG 1 cut(s) 356
MwoI GCNNNNNNNGC 2 cut(s) 188, 917
NarI GGCGCC 1 cut(s) 1343
NciI CCSGG 1 cut(s) 94
NdeII GATC 6 cut(s) 57, 280, 289, 1135, 1281, 1362
NlaIII CATG 7 cut(s) 40, 640, 856, 911, 987, 1049, 1193
NlaIV GGNNCC 2 cut(s) 371, 1344
NmuCI GTSAC 4 cut(s) 392, 707, 821, 1303
NsbI TGCGCA 1 cut(s) 857
NspI RCATGY 3 cut(s) 856, 987, 1193
PaeR7I CTCGAG 1 cut(s) 1353
PagI TCATGA 2 cut(s) 636, 1045
PceI AGGCCT 1 cut(s) 939
PciI ACATGT 1 cut(s) 1189
PciSI GCTCTTC 1 cut(s) 326
PctI GAATGC 2 cut(s) 195, 1055
PfeI GAWTC 4 cut(s) 651, 803, 944, 1239
PflFI GACNNNGTC 1 cut(s) 834
PkrI GCNGC 2 cut(s) 29, 32
PleI GAGTC 1 cut(s) 1319
PluTI GGCGCC 1 cut(s) 1346
PpsI GAGTC 1 cut(s) 1319
PscI ACATGT 1 cut(s) 1189
Psp1406I AACGTT 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 714
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 371, 1344
PstNI CAGNNNCTG 1 cut(s) 1027
PsyI GACNNNGTC 1 cut(s) 834
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 3 cut(s) 353, 1213, 1288
RsaNI GTAC 3 cut(s) 352, 1212, 1287
RseI CAYNNNNRTG 3 cut(s) 851, 1194, 1272
SapI GCTCTTC 1 cut(s) 326
SaqAI TTAA 8 cut(s) 309, 441, 536, 698, 780, 810, 1194, 1319
SatI GCNGC 2 cut(s) 28, 31
Sau3AI GATC 6 cut(s) 57, 280, 289, 1135, 1281, 1362
SchI GAGTC 1 cut(s) 1319
ScrFI CCNGG 2 cut(s) 94, 356
SduI GDGCHC 1 cut(s) 744
SfaNI GCATC 3 cut(s) 588, 1033, 1276
SfoI GGCGCC 1 cut(s) 1344
Sfr274I CTCGAG 1 cut(s) 1353
SlaI CTCGAG 1 cut(s) 1353
SmiMI CAYNNNNRTG 3 cut(s) 851, 1194, 1272
SmlI CTYRAG 6 cut(s) 308, 316, 896, 962, 1262, 1353
SmoI CTYRAG 6 cut(s) 308, 316, 896, 962, 1262, 1353
SseBI AGGCCT 1 cut(s) 939
SsiI CCGC 2 cut(s) 180, 1053
SspDI GGCGCC 1 cut(s) 1342
SspMI CTAG 1 cut(s) 1257
StuI AGGCCT 1 cut(s) 939
StyD4I CCNGG 2 cut(s) 92, 354
TaaI ACNGT 1 cut(s) 398
TaiI ACGT 4 cut(s) 298, 441, 602, 1186
TaqI TCGA 9 cut(s) 249, 292, 582, 684, 786, 942, 947, 1354, 1361
TatI WGTACW 1 cut(s) 1211
TfiI GAWTC 4 cut(s) 651, 803, 944, 1239
Tru1I TTAA 8 cut(s) 309, 441, 536, 698, 780, 810, 1194, 1319
Tru9I TTAA 8 cut(s) 309, 441, 536, 698, 780, 810, 1194, 1319
TscAI CASTG 1 cut(s) 652
TseFI GTSAC 4 cut(s) 392, 707, 821, 1303
TseI GCWGC 2 cut(s) 27, 30
Tsp45I GTSAC 4 cut(s) 392, 707, 821, 1303
TspDTI ATGAA 4 cut(s) 273, 625, 1034, 1062
TspGWI ACGGA 4 cut(s) 362, 806, 1240, 1251
TspRI CASTG 1 cut(s) 652
Tth111I GACNNNGTC 1 cut(s) 834
Vha464I CTTAAG 1 cut(s) 308
XapI RAATTY 5 cut(s) 632, 756, 812, 1090, 1159
XceI RCATGY 3 cut(s) 856, 987, 1193
XcmI CCANNNNNNNNNTGG 1 cut(s) 547
XhoI CTCGAG 1 cut(s) 1353
XmiI GTMKAC 1 cut(s) 1186
XspI CTAG 1 cut(s) 1257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.