Rh4AG114100

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000687
Physical Location & Seq
Forward (+)
24650619 .. 24673676
23058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG114100.1

Sequence Viewer

Length: 1239 bp
ATGAAACCTTGGAAGATGATCAAAGCTAGCTCAGGGTCTGATAACCCACCATGGAAGAAGCGGGTGCCCAAACCTTTGGGGGTTGAGGTGAAGCGAGCTTTGGAGCTGGTCGATGATGATCTCCCAGTTGAGGAGATTATGGATACCGAAGGAAAGGATGGATGGCAAGAGGCTGTGTTAAATGCTGGGGTGGAGATGAGGTGGAAATACAGATTCGATTATCCACCTCAGACTGAAGAGCTATGGTGGAAATCTTTCGATTCTGCAGCTCTCAAGTACAGTTTATCTCATTCTCTGATGTTGAGTACCTGGAATCCGCCGTTTTCCTGTGGGCAAGGGTTAGGACTAGGCAGCAAAGGTTTGTTTGAGGATGAGAAAGCACACATGAGAGCAGCCAGCCTTTCTTCTACTGACGGATACCCATCTGAATTGTGGGAAGGCACTACTGGAAATGCTATTATTGCTCTTACCGTCATCCCGAAGAGGAAGTACTTACACAATGGCAATAACCAGAGGCATCACCAGGAGAAACCAGAACAAGAACGAAAATGCCAACTTGGGCTTATCGGACCTCGTGATCAACGTGATCATGTGTCTCCTTTCCTCCAGACTAGCCGCCCGGAGCAGCATTCTCAGAAAACAATGGGCGAGCTGGCTCGGACGTCATACTTTCTAGTTCAATCGTTGAAAAATTTCTGCTGTAACATCAAAATCAAAGTTGAAGCACCTAATCTTGAAGTTTTCGGACCCATTGGTCAGAGTTTCAAGATCTTGCACCTGAGCAATGAGGAGGACTCCGACATTGCCTCTTGTCGAAAACTGAAGAATCTGGAGTTCGTTAGCTCAATATTCGTTTACGAATGGTTCGAGGGACTCGATTCCAGATTTCCACTGCTCGAGAGCGTGATTTTACAATATTGCGTGCCCTTGGAACATATCAATGTGGTTAGTCGAAGCCTGCAACGGTTTTCCTATTTTGATTATGGAGGGAATATGTTTCCTATACAATGGTTGATTGGAAACTTTGACTACTGCGAGAAGATGGGGTTGCATGCTATTGATGACAAGGCTCTTAGTTTTCCGTTGGATAAGAGGAATACCTGCTCTCCACCGTTGCCTCGTGTCCGGCTTCTCAAAGTGAAGATCGATGCTCCTACAACAAAACAAGTTAGGGAGTTGAAGGAATCCTTGAAATGGATGGCACCTTCATTAACGTGTTTATCTATAGAGCATAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

412

Amino Acids

47.21

Weight (kDa)

7.59

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 753
AatII GACGTC 1 cut(s) 665
Acc36I ACCTGC 1 cut(s) 1109
AccB1I GGYRCC 2 cut(s) 64, 1201
AciI CCGC 3 cut(s) 61, 317, 616
AcsI RAATTY 1 cut(s) 691
AcuI CTGAAG 2 cut(s) 255, 842
AcyI GRCGYC 1 cut(s) 662
AfaI GTAC 3 cut(s) 278, 307, 491
AfiI CCNNNNNNNGG 2 cut(s) 130, 1194
AflIII ACRYGT 1 cut(s) 1214
AgsI TTSAA 7 cut(s) 680, 688, 722, 737, 766, 1180, 1192
AjnI CCWGG 2 cut(s) 308, 522
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AluBI AGCT 8 cut(s) 26, 30, 98, 106, 241, 269, 652, 843
AluI AGCT 8 cut(s) 26, 30, 98, 106, 241, 269, 652, 843
Alw26I GTCTC 1 cut(s) 600
AlwNI CAGNNNCTG 1 cut(s) 38
Ama87I CYCGRG 1 cut(s) 896
ApeKI GCWGC 4 cut(s) 266, 351, 392, 625
ApoI RAATTY 1 cut(s) 691
Asp700I GAANNNNTTC 2 cut(s) 254, 692
AspS9I GGNCC 2 cut(s) 569, 746
AsuC2I CCSGG 1 cut(s) 620
AsuHPI GGTGA 2 cut(s) 100, 512
AsuNHI GCTAGC 1 cut(s) 26
AvaI CYCGRG 1 cut(s) 896
AvaII GGWCC 2 cut(s) 569, 746
BaeGI GKGCMC 2 cut(s) 69, 927
BanI GGYRCC 2 cut(s) 64, 1201
BarI GAAGNNNNNNTAC 2 cut(s) 473, 505
BauI CACGAG 2 cut(s) 573, 1119
BbvI GCAGC 4 cut(s) 278, 363, 404, 637
BccI CCATC 5 cut(s) 152, 156, 430, 1036, 1192
BceAI ACGGC 1 cut(s) 304
BciT130I CCWGG 2 cut(s) 310, 524
BciVI GTATCC 2 cut(s) 136, 410
BclI TGATCA 3 cut(s) 18, 577, 586
BcnI CCSGG 1 cut(s) 620
BcoDI GTCTC 1 cut(s) 600
BfaI CTAG 4 cut(s) 27, 347, 612, 674
BfmI CTRYAG 2 cut(s) 264, 1224
BfuAI ACCTGC 1 cut(s) 1109
BfuI GTATCC 2 cut(s) 136, 410
BglII AGATCT 1 cut(s) 768
BisI GCNGC 5 cut(s) 267, 352, 393, 616, 626
BlsI GCNGC 5 cut(s) 268, 353, 394, 617, 627
BmcAI AGTACT 1 cut(s) 491
Bme1390I CCNGG 3 cut(s) 310, 524, 620
Bme18I GGWCC 2 cut(s) 569, 746
BmeT110I CYCGRG 1 cut(s) 896
BmgT120I GGNCC 2 cut(s) 569, 746
BmiI GGNNCC 3 cut(s) 66, 748, 1203
BmrFI CCNGG 3 cut(s) 310, 524, 620
BmrI ACTGGG 1 cut(s) 119
BmsI GCATC 2 cut(s) 526, 1138
BmtI GCTAGC 1 cut(s) 30
BmuI ACTGGG 1 cut(s) 119
BplI GAGNNNNNCTC 2 cut(s) 779, 811
BpmI CTGGAG 2 cut(s) 590, 851
Bpu10I CCTNAGC 2 cut(s) 31, 779
BpuEI CTTGAG 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 620
Bsa29I ATCGAT 1 cut(s) 1146
BsaBI GATNNNNATC 2 cut(s) 117, 421
BsaHI GRCGYC 1 cut(s) 662
BsaJI CCNNGG 3 cut(s) 8, 50, 927
BsaXI ACNNNNNCTCC 4 cut(s) 185, 215, 1090, 1120
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 1194
Bse1I ACTGG 2 cut(s) 125, 451
Bse3DI GCAATG 2 cut(s) 790, 801
Bse8I GATNNNNATC 2 cut(s) 117, 421
BseBI CCWGG 2 cut(s) 310, 524
BseCI ATCGAT 1 cut(s) 1146
BseDI CCNNGG 3 cut(s) 8, 50, 927
BseGI GGATG 5 cut(s) 163, 167, 376, 474, 1203
BseJI GATNNNNATC 2 cut(s) 117, 421
BseLI CCNNNNNNNGG 2 cut(s) 130, 1194
BseMI GCAATG 2 cut(s) 790, 801
BseMII CTCAG 4 cut(s) 45, 242, 647, 770
BseNI ACTGG 2 cut(s) 125, 451
BseRI GAGGAG 2 cut(s) 146, 803
BseSI GKGCMC 2 cut(s) 69, 927
BseXI GCAGC 4 cut(s) 278, 363, 404, 637
BseYI CCCAGC 1 cut(s) 185
BshNI GGYRCC 2 cut(s) 64, 1201
BshVI ATCGAT 1 cut(s) 1146
BsiHKCI CYCGRG 1 cut(s) 896
BsiSI CCGG 2 cut(s) 620, 1126
BslFI GGGAC 1 cut(s) 885
BslI CCNNNNNNNGG 2 cut(s) 130, 1194
BsmAI GTCTC 1 cut(s) 600
BsmFI GGGAC 1 cut(s) 885
BsmI GAATGC 1 cut(s) 628
BsoBI CYCGRG 1 cut(s) 896
Bsp1286I GDGCHC 2 cut(s) 69, 927
Bsp143I GATC 6 cut(s) 18, 118, 577, 586, 768, 1143
Bsp19I CCATGG 1 cut(s) 50
BspACI CCGC 3 cut(s) 61, 317, 616
BspCNI CTCAG 4 cut(s) 44, 241, 646, 771
BspDI ATCGAT 1 cut(s) 1146
BspLI GGNNCC 3 cut(s) 66, 748, 1203
BspMAI CTGCAG 1 cut(s) 268
BspMI ACCTGC 1 cut(s) 1109
BspOI GCTAGC 1 cut(s) 30
BspQI GCTCTTC 1 cut(s) 231
BspT107I GGYRCC 2 cut(s) 64, 1201
BsrDI GCAATG 2 cut(s) 790, 801
BsrI ACTGG 2 cut(s) 125, 451
BssECI CCNNGG 3 cut(s) 8, 50, 927
BssMI GATC 6 cut(s) 18, 118, 577, 586, 768, 1143
BssNI GRCGYC 1 cut(s) 662
BssSI CACGAG 2 cut(s) 573, 1119
BssT1I CCWWGG 3 cut(s) 8, 50, 927
Bst2BI CACGAG 2 cut(s) 573, 1119
Bst2UI CCWGG 2 cut(s) 310, 524
Bst4CI ACNGT 4 cut(s) 281, 472, 966, 1113
Bst6I CTCTTC 2 cut(s) 231, 476
BstACI GRCGYC 1 cut(s) 662
BstC8I GCNNGC 8 cut(s) 28, 96, 397, 650, 654, 923, 959, 1053
BstDEI CTNAG 5 cut(s) 31, 228, 633, 779, 1073
BstDSI CCRYGG 1 cut(s) 50
BstF5I GGATG 5 cut(s) 163, 167, 376, 474, 1203
BstKTI GATC 6 cut(s) 21, 121, 580, 589, 771, 1146
BstMAI GTCTC 1 cut(s) 600
BstMBI GATC 6 cut(s) 18, 118, 577, 586, 768, 1143
BstMWI GCNNNNNNNGC 1 cut(s) 461
BstNI CCWGG 2 cut(s) 310, 524
BstNSI RCATGY 1 cut(s) 1055
BstSCI CCNGG 3 cut(s) 308, 522, 618
BstSFI CTRYAG 2 cut(s) 264, 1224
BstSLI GKGCMC 2 cut(s) 69, 927
BstV1I GCAGC 4 cut(s) 278, 363, 404, 637
BstX2I RGATCY 1 cut(s) 768
BstXI CCANNNNNNTGG 1 cut(s) 76
BstYI RGATCY 1 cut(s) 768
Bsu15I ATCGAT 1 cut(s) 1146
BsuI GTATCC 2 cut(s) 136, 410
BsuTUI ATCGAT 1 cut(s) 1146
BtgI CCRYGG 1 cut(s) 50
BtsCI GGATG 5 cut(s) 163, 167, 376, 474, 1203
BtsI GCAGTG 1 cut(s) 890
BtsIMutI CAGTG 1 cut(s) 890
BveI ACCTGC 1 cut(s) 1109
Cac8I GCNNGC 8 cut(s) 28, 96, 397, 650, 654, 923, 959, 1053
CaiI CAGNNNCTG 1 cut(s) 38
Cfr13I GGNCC 2 cut(s) 569, 746
ClaI ATCGAT 1 cut(s) 1146
Csp6I GTAC 3 cut(s) 277, 306, 490
CviAII CATG 4 cut(s) 51, 385, 590, 1052
CviQI GTAC 3 cut(s) 277, 306, 490
DdeI CTNAG 5 cut(s) 31, 228, 633, 779, 1073
DpnI GATC 6 cut(s) 20, 120, 579, 588, 770, 1145
DpnII GATC 6 cut(s) 18, 118, 577, 586, 768, 1143
DrdI GACNNNNNNGTC 1 cut(s) 753
DseDI GACNNNNNNGTC 1 cut(s) 753
Eam1104I CTCTTC 2 cut(s) 231, 476
EarI CTCTTC 2 cut(s) 231, 476
EciI GGCGGA 1 cut(s) 306
Eco130I CCWWGG 3 cut(s) 8, 50, 927
Eco47I GGWCC 2 cut(s) 569, 746
Eco57I CTGAAG 2 cut(s) 255, 842
Eco88I CYCGRG 1 cut(s) 896
EcoRII CCWGG 2 cut(s) 308, 522
EcoT14I CCWWGG 3 cut(s) 8, 50, 927
ErhI CCWWGG 3 cut(s) 8, 50, 927
FaeI CATG 4 cut(s) 54, 388, 593, 1055
FalI AAGNNNNNCTT 2 cut(s) 1172, 1204
FaqI GGGAC 1 cut(s) 885
FatI CATG 4 cut(s) 50, 384, 589, 1051
FauI CCCGC 1 cut(s) 54
FbaI TGATCA 3 cut(s) 18, 577, 586
Fnu4HI GCNGC 5 cut(s) 267, 352, 393, 616, 626
FokI GGATG 5 cut(s) 170, 174, 383, 461, 1210
Fsp4HI GCNGC 5 cut(s) 267, 352, 393, 616, 626
FspBI CTAG 4 cut(s) 27, 347, 612, 674
GluI GCNGC 5 cut(s) 267, 352, 393, 616, 626
GsaI CCCAGC 1 cut(s) 189
GsuI CTGGAG 2 cut(s) 590, 851
HapII CCGG 2 cut(s) 620, 1126
Hin1I GRCGYC 1 cut(s) 662
Hin1II CATG 4 cut(s) 54, 388, 593, 1055
HinfI GANTC 8 cut(s) 213, 260, 313, 794, 826, 873, 878, 1184
HpaII CCGG 2 cut(s) 620, 1126
HphI GGTGA 2 cut(s) 100, 512
Hpy166II GTNNAC 1 cut(s) 856
Hpy188III TCNNGA 8 cut(s) 478, 575, 607, 734, 766, 830, 882, 898
Hpy8I GTNNAC 1 cut(s) 856
HpyAV CCTTC 4 cut(s) 143, 431, 1174, 1215
HpyCH4III ACNGT 4 cut(s) 281, 472, 966, 1113
HpyCH4IV ACGT 3 cut(s) 583, 662, 1214
HpyCH4V TGCA 4 cut(s) 266, 775, 961, 1051
HpyF10VI GCNNNNNNNGC 1 cut(s) 461
HpyF3I CTNAG 5 cut(s) 31, 228, 633, 779, 1073
HpySE526I ACGT 3 cut(s) 583, 662, 1214
Hsp92I GRCGYC 1 cut(s) 662
Hsp92II CATG 4 cut(s) 54, 388, 593, 1055
Ksp22I TGATCA 3 cut(s) 18, 577, 586
Kzo9I GATC 6 cut(s) 18, 118, 577, 586, 768, 1143
LguI GCTCTTC 1 cut(s) 231
LmnI GCTCC 3 cut(s) 103, 622, 1156
Lsp1109I GCAGC 4 cut(s) 278, 363, 404, 637
LweI GCATC 2 cut(s) 526, 1138
MaeI CTAG 4 cut(s) 27, 347, 612, 674
MaeII ACGT 3 cut(s) 583, 662, 1214
MaeIII GTNAC 1 cut(s) 701
MalI GATC 6 cut(s) 20, 120, 579, 588, 770, 1145
MboI GATC 6 cut(s) 18, 118, 577, 586, 768, 1143
MboII GAAGA 8 cut(s) 25, 67, 248, 396, 493, 835, 1051, 1153
MflI RGATCY 1 cut(s) 768
MhlI GDGCHC 2 cut(s) 69, 927
MluCI AATT 2 cut(s) 428, 691
MlyI GAGTC 2 cut(s) 788, 867
MmeI TCCRAC 2 cut(s) 822, 1065
MroXI GAANNNNTTC 2 cut(s) 254, 692
MseI TTAA 2 cut(s) 179, 1211
MslI CAYNNNNRTG 2 cut(s) 939, 1213
MspI CCGG 2 cut(s) 620, 1126
MspR9I CCNGG 3 cut(s) 310, 524, 620
Mva1269I GAATGC 1 cut(s) 628
MvaI CCWGG 2 cut(s) 310, 524
MwoI GCNNNNNNNGC 1 cut(s) 461
NciI CCSGG 1 cut(s) 620
NcoI CCATGG 1 cut(s) 50
NdeII GATC 6 cut(s) 18, 118, 577, 586, 768, 1143
NheI GCTAGC 1 cut(s) 26
NlaIII CATG 4 cut(s) 54, 388, 593, 1055
NlaIV GGNNCC 3 cut(s) 66, 748, 1203
NspI RCATGY 1 cut(s) 1055
PaeI GCATGC 1 cut(s) 1055
PaeR7I CTCGAG 1 cut(s) 896
PciSI GCTCTTC 1 cut(s) 231
PcsI WCGNNNNNNNCGW 3 cut(s) 580, 864, 873
PctI GAATGC 1 cut(s) 628
PdmI GAANNNNTTC 2 cut(s) 254, 692
PfeI GAWTC 6 cut(s) 213, 260, 313, 826, 878, 1184
PkrI GCNGC 5 cut(s) 268, 353, 394, 617, 627
PleI GAGTC 2 cut(s) 788, 867
PpsI GAGTC 2 cut(s) 788, 867
Psp6I CCWGG 2 cut(s) 308, 522
PspFI CCCAGC 1 cut(s) 185
PspGI CCWGG 2 cut(s) 308, 522
PspN4I GGNNCC 3 cut(s) 66, 748, 1203
PspPI GGNCC 2 cut(s) 569, 746
PstI CTGCAG 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 38
PsuI RGATCY 1 cut(s) 768
RsaI GTAC 3 cut(s) 278, 307, 491
RsaNI GTAC 3 cut(s) 277, 306, 490
RseI CAYNNNNRTG 2 cut(s) 939, 1213
SapI GCTCTTC 1 cut(s) 231
SaqAI TTAA 2 cut(s) 179, 1211
SatI GCNGC 5 cut(s) 267, 352, 393, 616, 626
Sau3AI GATC 6 cut(s) 18, 118, 577, 586, 768, 1143
Sau96I GGNCC 2 cut(s) 569, 746
ScaI AGTACT 1 cut(s) 491
SchI GAGTC 2 cut(s) 788, 867
ScrFI CCNGG 3 cut(s) 310, 524, 620
SduI GDGCHC 2 cut(s) 69, 927
SfaNI GCATC 2 cut(s) 526, 1138
SfcI CTRYAG 2 cut(s) 264, 1224
Sfr274I CTCGAG 1 cut(s) 896
SinI GGWCC 2 cut(s) 569, 746
SlaI CTCGAG 1 cut(s) 896
SmiMI CAYNNNNRTG 2 cut(s) 939, 1213
SmlI CTYRAG 2 cut(s) 272, 896
SmoI CTYRAG 2 cut(s) 272, 896
SphI GCATGC 1 cut(s) 1055
Sse9I AATT 2 cut(s) 428, 691
SsiI CCGC 3 cut(s) 61, 317, 616
SspI AATATT 2 cut(s) 849, 917
SspMI CTAG 4 cut(s) 27, 347, 612, 674
StyD4I CCNGG 3 cut(s) 308, 522, 618
StyI CCWWGG 3 cut(s) 8, 50, 927
TaaI ACNGT 4 cut(s) 281, 472, 966, 1113
TaiI ACGT 3 cut(s) 586, 665, 1217
TaqI TCGA 9 cut(s) 111, 216, 258, 814, 867, 876, 897, 952, 1146
TasI AATT 2 cut(s) 428, 691
TatI WGTACW 2 cut(s) 276, 489
TauI GCSGC 1 cut(s) 618
TfiI GAWTC 6 cut(s) 213, 260, 313, 826, 878, 1184
Tru1I TTAA 2 cut(s) 179, 1211
Tru9I TTAA 2 cut(s) 179, 1211
TscAI CASTG 1 cut(s) 897
TseI GCWGC 4 cut(s) 266, 351, 392, 625
TspDTI ATGAA 2 cut(s) 17, 1197
TspGWI ACGGA 2 cut(s) 429, 1071
TspRI CASTG 1 cut(s) 897
VpaK11BI GGWCC 2 cut(s) 569, 746
XapI RAATTY 1 cut(s) 691
XceI RCATGY 1 cut(s) 1055
XcmI CCANNNNNNNNNTGG 1 cut(s) 429
XhoI CTCGAG 1 cut(s) 896
XmnI GAANNNNTTC 2 cut(s) 254, 692
XspI CTAG 4 cut(s) 27, 347, 612, 674
ZraI GACGTC 1 cut(s) 663
ZrmI AGTACT 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.