Rroxscaffold_2G00082790

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000687
Physical Location & Seq
Forward (+)
5550283 .. 5551965
1683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082790.1

Sequence Viewer

Length: 1305 bp
ATGGAGCAAATCCTCGCTGGATCTCTGCTTTCCACCAAAAACACTGCCCGGACATGCATTTTTTCTAAGCAGTGGTTACGTGTATGGCCTTTGATTCCTGCATTGGACATTGATGAGGATGAGGATGAGAACAATGAGGACGATGAGGATGAGCAGGCAGGCCTTGCGAAAAACGGGATTCGACATCAAAGGTTCATGAACTTCTTGAGCATGTGTTTGAAGCGATGTCAGAAATATAACAACACGTTAGCGTTAGACAAGTTCAGGCTTCGTATGAGATACTTTGGTGATTGGAACTATGATGATGAGATCATAAGCAGGTGGTTGAGTTATGCTGCTGAAAGAAACAGAAACGTTAAAGAGTTAGACATCTGCTTCATATATATACCTGATTTACCGGCATACACCCCGTACCGCTTACCACGAACACTTCTCAGCAACCATGCAGGATCTTTAACCATTCTAAAGTTGGAGAATGTGGTGATACAGGGCAGCAATGACCCTACGAGCTTCCCATGTTTGAGAACTATGTCCCTCAAACTAGTGGAATTCAGTAGCAACTTCGCATTCTTGCAGTTAATTTCCGGGTGCCCTTTCGTGGCTTACTTGTCCCTCAAGCTATGTACTGGCTTGTCACAGACTCTTAAACTTTCTAGTTTGAGCCTCGAATCCTTGGAAGTTATGCATTGCGGCTCAGTGCGTTTTCAAATTGAAGCTACGAATCTTAAATCTTTTAAACTACATGGAGGGCTGTACAGCTGGGGCAACGTTGATATTGCCTCTTGTAAAAATGTTGAGAGCCTAGAGTTCTTTGATACATGCTTGACAGAAACTTGGCTTAGAGGCTTTGCTTCAACGTTTCCCCTCCTGGAGAGTTTGGTTTTACATGACTACTGGGAAGGACGAATGAAAAATACCAAAATCATTCCAAACTTATTGGATGCTATAATTTCAATCCATATAGGGAGGGAGCCTAGCTCGTCTTGGAATCATCTTCGTCTGATTAGATTTCTTGAGAATTTTGATTGCTTCAGAAAATTAAGTCTGCATGCTGCTGCTCCTGAGGCTCTCATCTTTCCAGAAGAAGTGAGAAGGTGTCCACCATTAACAAACCTCGAGCATCTTCAGGTAGAGATTAGTTTTACAACCACAGTAAAGATTTTCTCATTGCTGATGGACTCCTTGCATTGGATGGCACCATCTGCTGCGGTTTCTATCCACAGAACAGATTGCGATCCCTATCCAGAAACAGAATTACTCAAAACAATAATCGGTTCATTTATGAAAACTGATCTCGGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

434

Amino Acids

49.73

Weight (kDa)

5.82

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 106 - 275 5.4e-12 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 309
Acc36I ACCTGC 1 cut(s) 309
AccB1I GGYRCC 2 cut(s) 588, 1195
AciI CCGC 3 cut(s) 415, 690, 1208
AclI AACGTT 3 cut(s) 354, 768, 857
AclWI GGATC 3 cut(s) 28, 457, 1229
AcsI RAATTY 2 cut(s) 548, 1018
AcuI CTGAAG 2 cut(s) 1015, 1109
AfaI GTAC 3 cut(s) 413, 625, 755
AfiI CCNNNNNNNGG 2 cut(s) 598, 1188
AflIII ACRYGT 2 cut(s) 79, 243
AgsI TTSAA 5 cut(s) 220, 707, 713, 855, 954
AhlI ACTAGT 1 cut(s) 541
AjnI CCWGG 1 cut(s) 867
AluBI AGCT 5 cut(s) 510, 619, 716, 759, 978
AluI AGCT 5 cut(s) 510, 619, 716, 759, 978
AlwI GGATC 3 cut(s) 28, 457, 1229
Ama87I CYCGRG 1 cut(s) 1115
AoxI GGCC 2 cut(s) 86, 160
ApeKI GCWGC 5 cut(s) 335, 492, 1052, 1055, 1205
ApoI RAATTY 2 cut(s) 548, 1018
AsuC2I CCSGG 2 cut(s) 49, 586
AsuHPI GGTGA 2 cut(s) 299, 493
AvaI CYCGRG 1 cut(s) 1115
AxyI CCTNAGG 1 cut(s) 1062
BaeGI GKGCMC 1 cut(s) 593
BaeI ACNNNNGTAYC 2 cut(s) 271, 304
BanI GGYRCC 2 cut(s) 588, 1195
BbvI GCAGC 5 cut(s) 322, 504, 1039, 1042, 1192
BccI CCATC 3 cut(s) 1168, 1186, 1207
BciT130I CCWGG 1 cut(s) 869
BcnI CCSGG 2 cut(s) 49, 586
BcuI ACTAGT 1 cut(s) 541
BfaI CTAG 4 cut(s) 542, 654, 803, 975
BfuAI ACCTGC 1 cut(s) 309
BisI GCNGC 6 cut(s) 336, 493, 691, 1053, 1056, 1206
BlsI GCNGC 6 cut(s) 337, 494, 692, 1054, 1057, 1207
Bme1390I CCNGG 3 cut(s) 49, 586, 869
BmeT110I CYCGRG 1 cut(s) 1115
BmiI GGNNCC 3 cut(s) 590, 972, 1197
BmrFI CCNGG 3 cut(s) 49, 586, 869
BmrI ACTGGG 1 cut(s) 904
BmsI GCATC 2 cut(s) 931, 1129
BmuI ACTGGG 1 cut(s) 904
BplI GAGNNNNNCTC 2 cut(s) 962, 994
BpmI CTGGAG 1 cut(s) 890
BpuEI CTTGAG 3 cut(s) 226, 599, 1034
BpuMI CCSGG 2 cut(s) 49, 586
BsaAI YACGTR 1 cut(s) 80
BsaBI GATNNNNATC 2 cut(s) 1233, 1239
BsaJI CCNNGG 1 cut(s) 672
Bsc4I CCNNNNNNNGG 2 cut(s) 598, 1188
Bse118I RCCGGY 1 cut(s) 397
Bse1I ACTGG 2 cut(s) 631, 899
Bse21I CCTNAGG 1 cut(s) 1062
Bse3DI GCAATG 3 cut(s) 502, 685, 1166
Bse8I GATNNNNATC 2 cut(s) 1233, 1239
BseBI CCWGG 1 cut(s) 869
BseDI CCNNGG 1 cut(s) 672
BseGI GGATG 5 cut(s) 124, 130, 154, 946, 1197
BseJI GATNNNNATC 2 cut(s) 1233, 1239
BseLI CCNNNNNNNGG 2 cut(s) 598, 1188
BseMI GCAATG 3 cut(s) 502, 685, 1166
BseMII CTCAG 3 cut(s) 448, 708, 1053
BseNI ACTGG 2 cut(s) 631, 899
BseSI GKGCMC 1 cut(s) 593
BseXI GCAGC 5 cut(s) 322, 504, 1039, 1042, 1192
BseYI CCCAGC 1 cut(s) 759
BshFI GGCC 2 cut(s) 88, 162
BshNI GGYRCC 2 cut(s) 588, 1195
BsiHKCI CYCGRG 1 cut(s) 1115
BsiSI CCGG 3 cut(s) 49, 398, 585
BslFI GGGAC 2 cut(s) 517, 595
BslI CCNNNNNNNGG 2 cut(s) 598, 1188
BsmFI GGGAC 2 cut(s) 517, 595
BsmI GAATGC 1 cut(s) 566
BsnI GGCC 2 cut(s) 88, 162
BsoBI CYCGRG 1 cut(s) 1115
Bsp1286I GDGCHC 1 cut(s) 593
Bsp1407I TGTACA 1 cut(s) 753
Bsp143I GATC 5 cut(s) 20, 309, 449, 1234, 1291
BspACI CCGC 3 cut(s) 415, 690, 1208
BspANI GGCC 2 cut(s) 88, 162
BspCNI CTCAG 3 cut(s) 447, 707, 1054
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 3 cut(s) 590, 972, 1197
BspMI ACCTGC 1 cut(s) 309
BspPI GGATC 3 cut(s) 28, 457, 1229
BspT107I GGYRCC 2 cut(s) 588, 1195
BsrDI GCAATG 3 cut(s) 502, 685, 1166
BsrFI RCCGGY 1 cut(s) 397
BsrGI TGTACA 1 cut(s) 753
BsrI ACTGG 2 cut(s) 631, 899
BssAI RCCGGY 1 cut(s) 397
BssECI CCNNGG 1 cut(s) 672
BssMI GATC 5 cut(s) 20, 309, 449, 1234, 1291
BssT1I CCWWGG 1 cut(s) 672
Bst2UI CCWGG 1 cut(s) 869
Bst4CI ACNGT 1 cut(s) 1153
BstAPI GCANNNNNTGC 2 cut(s) 164, 1202
BstAUI TGTACA 1 cut(s) 753
BstBAI YACGTR 1 cut(s) 80
BstC8I GCNNGC 3 cut(s) 156, 160, 1050
BstDEI CTNAG 5 cut(s) 66, 434, 694, 839, 1062
BstF5I GGATG 5 cut(s) 124, 130, 154, 946, 1197
BstKTI GATC 5 cut(s) 23, 312, 452, 1237, 1294
BstMBI GATC 5 cut(s) 20, 309, 449, 1234, 1291
BstMWI GCNNNNNNNGC 3 cut(s) 164, 1064, 1202
BstNI CCWGG 1 cut(s) 869
BstNSI RCATGY 4 cut(s) 57, 214, 822, 1052
BstSCI CCNGG 3 cut(s) 47, 584, 867
BstSLI GKGCMC 1 cut(s) 593
BstV1I GCAGC 5 cut(s) 322, 504, 1039, 1042, 1192
BstX2I RGATCY 2 cut(s) 20, 449
BstYI RGATCY 2 cut(s) 20, 449
Bsu36I CCTNAGG 1 cut(s) 1062
BsuRI GGCC 2 cut(s) 88, 162
BtgZI GCGATG 1 cut(s) 238
BtsCI GGATG 5 cut(s) 124, 130, 154, 946, 1197
BtsI GCAGTG 2 cut(s) 42, 77
BtsIMutI CAGTG 3 cut(s) 42, 77, 702
BveI ACCTGC 1 cut(s) 309
Cac8I GCNNGC 3 cut(s) 156, 160, 1050
CciI TCATGA 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 397
Csp6I GTAC 3 cut(s) 412, 624, 754
CviAII CATG 9 cut(s) 54, 196, 211, 443, 516, 743, 819, 887, 1049
CviQI GTAC 3 cut(s) 412, 624, 754
DdeI CTNAG 5 cut(s) 66, 434, 694, 839, 1062
DpnI GATC 5 cut(s) 22, 311, 451, 1236, 1293
DpnII GATC 5 cut(s) 20, 309, 449, 1234, 1291
DraI TTTAAA 1 cut(s) 736
Eco130I CCWWGG 1 cut(s) 672
Eco147I AGGCCT 1 cut(s) 162
Eco57I CTGAAG 2 cut(s) 1015, 1109
Eco81I CCTNAGG 1 cut(s) 1062
Eco88I CYCGRG 1 cut(s) 1115
EcoRI GAATTC 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 867
EcoT14I CCWWGG 1 cut(s) 672
EcoT22I ATGCAT 2 cut(s) 59, 687
ErhI CCWWGG 1 cut(s) 672
FaeI CATG 9 cut(s) 57, 199, 214, 446, 519, 746, 822, 890, 1052
FaqI GGGAC 2 cut(s) 517, 595
FatI CATG 9 cut(s) 53, 195, 210, 442, 515, 742, 818, 886, 1048
Fnu4HI GCNGC 6 cut(s) 336, 493, 691, 1053, 1056, 1206
FokI GGATG 5 cut(s) 131, 137, 161, 953, 1204
Fsp4HI GCNGC 6 cut(s) 336, 493, 691, 1053, 1056, 1206
FspBI CTAG 4 cut(s) 542, 654, 803, 975
GluI GCNGC 6 cut(s) 336, 493, 691, 1053, 1056, 1206
GsaI CCCAGC 1 cut(s) 763
GsuI CTGGAG 1 cut(s) 890
HaeIII GGCC 2 cut(s) 88, 162
HapII CCGG 3 cut(s) 49, 398, 585
Hin1II CATG 9 cut(s) 57, 199, 214, 446, 519, 746, 822, 890, 1052
HinfI GANTC 7 cut(s) 94, 178, 640, 668, 721, 988, 1178
HpaII CCGG 3 cut(s) 49, 398, 585
HphI GGTGA 2 cut(s) 299, 493
Hpy166II GTNNAC 1 cut(s) 1100
Hpy188I TCNGA 3 cut(s) 231, 1002, 1034
Hpy188III TCNNGA 6 cut(s) 196, 205, 1013, 1061, 1079, 1244
Hpy8I GTNNAC 1 cut(s) 1100
HpyAV CCTTC 2 cut(s) 893, 1086
HpyCH4III ACNGT 1 cut(s) 1153
HpyCH4IV ACGT 5 cut(s) 79, 245, 354, 768, 857
HpyCH4V TGCA 7 cut(s) 57, 101, 446, 574, 685, 1048, 1186
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 1064, 1202
HpyF3I CTNAG 5 cut(s) 66, 434, 694, 839, 1062
HpySE526I ACGT 5 cut(s) 79, 245, 354, 768, 857
Hsp92II CATG 9 cut(s) 57, 199, 214, 446, 519, 746, 822, 890, 1052
Kzo9I GATC 5 cut(s) 20, 309, 449, 1234, 1291
LmnI GCTCC 3 cut(s) 4, 970, 1063
Lsp1109I GCAGC 5 cut(s) 322, 504, 1039, 1042, 1192
LweI GCATC 2 cut(s) 931, 1129
MaeI CTAG 4 cut(s) 542, 654, 803, 975
MaeII ACGT 5 cut(s) 79, 245, 354, 768, 857
MaeIII GTNAC 2 cut(s) 75, 633
MalI GATC 5 cut(s) 22, 311, 451, 1236, 1293
MboI GATC 5 cut(s) 20, 309, 449, 1234, 1291
MboII GAAGA 3 cut(s) 986, 1094, 1115
MflI RGATCY 2 cut(s) 20, 449
MhlI GDGCHC 1 cut(s) 593
MluCI AATT 7 cut(s) 548, 579, 708, 948, 1018, 1037, 1253
MlyI GAGTC 2 cut(s) 634, 1172
MmeI TCCRAC 1 cut(s) 450
Mph1103I ATGCAT 2 cut(s) 59, 687
MseI TTAA 8 cut(s) 357, 455, 578, 645, 726, 735, 1040, 1106
MspA1I CMGCKG 1 cut(s) 759
MspI CCGG 3 cut(s) 49, 398, 585
MspR9I CCNGG 3 cut(s) 49, 586, 869
Mva1269I GAATGC 1 cut(s) 566
MvaI CCWGG 1 cut(s) 869
MwoI GCNNNNNNNGC 3 cut(s) 164, 1064, 1202
NciI CCSGG 2 cut(s) 49, 586
NdeII GATC 5 cut(s) 20, 309, 449, 1234, 1291
NlaIII CATG 9 cut(s) 57, 199, 214, 446, 519, 746, 822, 890, 1052
NlaIV GGNNCC 3 cut(s) 590, 972, 1197
NmuCI GTSAC 1 cut(s) 633
NsiI ATGCAT 2 cut(s) 59, 687
NspI RCATGY 4 cut(s) 57, 214, 822, 1052
PaeI GCATGC 1 cut(s) 1052
PaeR7I CTCGAG 1 cut(s) 1115
PagI TCATGA 1 cut(s) 195
PaqCI CACCTGC 1 cut(s) 309
PceI AGGCCT 1 cut(s) 162
PctI GAATGC 1 cut(s) 566
PfeI GAWTC 5 cut(s) 94, 178, 668, 721, 988
PfoI TCCNGGA 1 cut(s) 867
PkrI GCNGC 6 cut(s) 337, 494, 692, 1054, 1057, 1207
PleI GAGTC 2 cut(s) 634, 1172
PpsI GAGTC 2 cut(s) 634, 1172
Ppu21I YACGTR 1 cut(s) 80
Psp1406I AACGTT 3 cut(s) 354, 768, 857
Psp6I CCWGG 1 cut(s) 867
PspFI CCCAGC 1 cut(s) 759
PspGI CCWGG 1 cut(s) 867
PspN4I GGNNCC 3 cut(s) 590, 972, 1197
PspXI VCTCGAGB 1 cut(s) 1115
PsuI RGATCY 2 cut(s) 20, 449
PvuII CAGCTG 1 cut(s) 759
RsaI GTAC 3 cut(s) 413, 625, 755
RsaNI GTAC 3 cut(s) 412, 624, 754
SaqAI TTAA 8 cut(s) 357, 455, 578, 645, 726, 735, 1040, 1106
SatI GCNGC 6 cut(s) 336, 493, 691, 1053, 1056, 1206
Sau3AI GATC 5 cut(s) 20, 309, 449, 1234, 1291
SchI GAGTC 2 cut(s) 634, 1172
ScrFI CCNGG 3 cut(s) 49, 586, 869
SduI GDGCHC 1 cut(s) 593
SfaNI GCATC 2 cut(s) 931, 1129
Sfr274I CTCGAG 1 cut(s) 1115
SlaI CTCGAG 1 cut(s) 1115
SmlI CTYRAG 4 cut(s) 205, 614, 1013, 1115
SmoI CTYRAG 4 cut(s) 205, 614, 1013, 1115
SpeI ACTAGT 1 cut(s) 541
SphI GCATGC 1 cut(s) 1052
Sse9I AATT 7 cut(s) 548, 579, 708, 948, 1018, 1037, 1253
SseBI AGGCCT 1 cut(s) 162
SsiI CCGC 3 cut(s) 415, 690, 1208
SspMI CTAG 4 cut(s) 542, 654, 803, 975
StuI AGGCCT 1 cut(s) 162
StyD4I CCNGG 3 cut(s) 47, 584, 867
StyI CCWWGG 1 cut(s) 672
TaaI ACNGT 1 cut(s) 1153
TaiI ACGT 5 cut(s) 82, 248, 357, 771, 860
TaqI TCGA 3 cut(s) 181, 666, 1116
TasI AATT 7 cut(s) 548, 579, 708, 948, 1018, 1037, 1253
TatI WGTACW 2 cut(s) 623, 753
TauI GCSGC 1 cut(s) 693
TfiI GAWTC 5 cut(s) 94, 178, 668, 721, 988
Tru1I TTAA 8 cut(s) 357, 455, 578, 645, 726, 735, 1040, 1106
Tru9I TTAA 8 cut(s) 357, 455, 578, 645, 726, 735, 1040, 1106
TscAI CASTG 3 cut(s) 49, 77, 702
TseFI GTSAC 1 cut(s) 633
TseI GCWGC 5 cut(s) 335, 492, 1052, 1055, 1205
Tsp45I GTSAC 1 cut(s) 633
TspDTI ATGAA 6 cut(s) 184, 212, 367, 923, 1266, 1298
TspRI CASTG 3 cut(s) 49, 77, 702
XapI RAATTY 2 cut(s) 548, 1018
XceI RCATGY 4 cut(s) 57, 214, 822, 1052
XcmI CCANNNNNNNNNTGG 1 cut(s) 466
XhoI CTCGAG 1 cut(s) 1115
XspI CTAG 4 cut(s) 542, 654, 803, 975
Zsp2I ATGCAT 2 cut(s) 59, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.