MD09G1093500.v1.1

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000687
Physical Location & Seq
Forward (+)
6839695 .. 6841322
1628 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1093500.v1.1.491

Sequence Viewer

Length: 1488 bp
ATGATTAAGAGAAAAGCCGAAGCTTGTAACGATATCAGAATCATAAATAAAAAGACCAAAAGCCAGGATCAGAATCATGACATTCAAAACCAACATGGTACTGCCGTGGTCACACGCGAGTTCGAATTACCCGACCTTGTTGTCTCCCAAGTTCTCCTTCTCCTCCCCACCAAACTCGCTGTTCGGGCCAGCATTCTTTCCAGGCAATGGGAACGAGTGTGGTCTTCGATCCCAGTCTTAGTTTTCAATGAGTATGAGGAGCCAGGCAGTGATTGGCACACCCTCGAGCACAAATTGTTCATTGACTTCTTGAAAAAGTGCTTGAAGCGTCGCGAGAAAGATAAATACACCGTGGACAAGCTTACGCTTCACATGAGGTATGGCGGAGGGAGAGGAAGCACGGCTATCATAGATAAGTGGACGAGTTTTGCTGTTGAGAGAAACATTAAAGAGATAGACATCATTCTTAAGAGAAAACTTGGCCCTGATAAAAATGCCATACACAAATTATCGCAAACCATTCTCAATGCAAAGTCTTTAACTACACTAAATTTAGAGAATGTGAGAATAGTGGACAATATGAGTTGTATAAGCCTTCCGTCTTTGGAATCTATGTCCCTCACGACCGTAGTGTTGAGTAAACTTGGATTCATCCAACTAATTTCTGGGTGCCCTTCCATTGAGTATTTGTCATTAAATCAATGTAGATTAAAGGATGATGTGAAGATTTCGAGTTTGAGCATCAAATCCTTGAAAGTTGTTCAATGTAGCATCGTGGGAATGGAAATTGAAGCTATGAATCTCGAATATTTTTTAAGTTGCTACACGTCCATGAGCAAAATCAATATTGCCTCTTGTCAAACACTTAGAAATCTGAACTTCTCTGATAGATGCTTGGAAGGTGCCTGGTTTGAACATGGCCTTGATTCAAGATTTCCACTCTTGGAGAGTTTAACAATGAATAACTGCATCATCTCATCGTCAATCATTAACGTCGATAATCAGCAGCTGAAACACATTGCACTTTATGGATACGACAAAAATGTAGCAAAGGTCACGCTTAGTACTCCAAATCTACTTTCTTTCGAGCTCAGTACTGCAGGTTATTTGTCTTCAGCTCAATTCGGTACTTTGGTGTCCAACTTTTCTATCAGTGCTCCAAAGTTGTTAGAGGCTATGATCATCATCAACTTCGATCCGATCGTCGATTCTTATTCGTCTTCCATGAGAAAATTTCTTGGGAACTTTGATTGCTCTAGAAAATTAAATCTTTCCGTTAATGCCGCTCAGGCTCTTATATTTTCGGAAGAAACCAGAAAGACATGCCAGCCACCGTTACCTACTGTCGAGCATCTTGAGGTACAAACATATTCTTCAGCAGACGTGAAAGAGGAGGACTTGGGGAGCTCCTTGGAGTGGTTGGCACCATCTGTGGATAGTCTACGCGTCACTGTGTATGAAGAAAATGAAACCTTTACACATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_2

Protein Analysis

496

Amino Acids

55.78

Weight (kDa)

6.8

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 120 - 343 6.7e-13 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 137 - 295 8.7e-13 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 140
Acc36I ACCTGC 1 cut(s) 1091
AccB1I GGYRCC 3 cut(s) 669, 902, 1423
AccB7I CCANNNNNTGG 1 cut(s) 207
AccBSI CCGCTC 1 cut(s) 1286
AccI GTMKAC 1 cut(s) 1441
AccII CGCG 3 cut(s) 117, 333, 1446
AciI CCGC 2 cut(s) 384, 1284
AclWI GGATC 3 cut(s) 75, 223, 1190
AcsI RAATTY 2 cut(s) 550, 1232
AcuI CTGAAG 2 cut(s) 1098, 1359
AfaI GTAC 5 cut(s) 100, 1066, 1096, 1129, 1362
AfiI CCNNNNNNNGG 2 cut(s) 207, 1416
AflII CTTAAG 1 cut(s) 467
AflIII ACRYGT 2 cut(s) 825, 1444
AgsI TTSAA 9 cut(s) 86, 247, 313, 325, 754, 764, 791, 914, 930
AjiI CACGTC 2 cut(s) 828, 1384
AjnI CCWGG 4 cut(s) 63, 200, 262, 905
AjuI GAANNNNNNNTTGG 4 cut(s) 141, 173, 1063, 1095
AluBI AGCT 7 cut(s) 23, 361, 794, 1009, 1090, 1118, 1407
AluI AGCT 7 cut(s) 23, 361, 794, 1009, 1090, 1118, 1407
Alw21I GWGCWC 4 cut(s) 291, 1092, 1159, 1409
Alw26I GTCTC 1 cut(s) 148
AlwI GGATC 3 cut(s) 75, 223, 1190
AlwNI CAGNNNCTG 1 cut(s) 1009
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 3 cut(s) 186, 481, 919
ApeKI GCWGC 1 cut(s) 1006
ApoI RAATTY 2 cut(s) 550, 1232
AspS9I GGNCC 2 cut(s) 186, 482
AsuII TTCGAA 1 cut(s) 123
AvaI CYCGRG 1 cut(s) 284
BaeGI GKGCMC 1 cut(s) 674
BaeI ACNNNNGTAYC 4 cut(s) 90, 123, 1119, 1152
BanI GGYRCC 3 cut(s) 669, 902, 1423
BanII GRGCYC 2 cut(s) 1092, 1409
BbsI GAAGAC 3 cut(s) 216, 1104, 1212
Bbv12I GWGCWC 4 cut(s) 291, 1092, 1159, 1409
BbvI GCAGC 1 cut(s) 1018
BccI CCATC 1 cut(s) 1435
BceAI ACGGC 2 cut(s) 89, 417
BciT130I CCWGG 4 cut(s) 65, 202, 264, 907
BciVI GTATCC 1 cut(s) 1025
BclI TGATCA 1 cut(s) 1179
BcoDI GTCTC 1 cut(s) 148
BfaI CTAG 1 cut(s) 1257
BfmI CTRYAG 1 cut(s) 1098
BfrI CTTAAG 1 cut(s) 467
BfuAI ACCTGC 1 cut(s) 1091
BfuI GTATCC 1 cut(s) 1025
BglI GCCNNNNNGGC 1 cut(s) 1289
BisI GCNGC 2 cut(s) 1007, 1284
BlsI GCNGC 2 cut(s) 1008, 1285
BmcAI AGTACT 2 cut(s) 1066, 1096
Bme1390I CCNGG 4 cut(s) 65, 202, 264, 907
BmeT110I CYCGRG 1 cut(s) 284
BmgBI CACGTC 2 cut(s) 828, 1384
BmgT120I GGNCC 2 cut(s) 186, 482
BmiI GGNNCC 4 cut(s) 261, 671, 904, 1425
BmrFI CCNGG 4 cut(s) 65, 202, 264, 907
BmrI ACTGGG 1 cut(s) 227
BmsI GCATC 5 cut(s) 750, 780, 881, 978, 1360
BmuI ACTGGG 1 cut(s) 227
BpiI GAAGAC 3 cut(s) 216, 1104, 1212
Bpu10I CCTNAGC 1 cut(s) 1287
Bpu14I TTCGAA 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 1376
BsaBI GATNNNNATC 3 cut(s) 72, 458, 1184
BsaJI CCNNGG 3 cut(s) 105, 351, 1410
Bsc4I CCNNNNNNNGG 2 cut(s) 207, 1416
Bse1I ACTGG 1 cut(s) 233
Bse3DI GCAATG 2 cut(s) 212, 1017
Bse8I GATNNNNATC 3 cut(s) 72, 458, 1184
BseBI CCWGG 4 cut(s) 65, 202, 264, 907
BseDI CCNNGG 3 cut(s) 105, 351, 1410
BseGI GGATG 2 cut(s) 651, 721
BseJI GATNNNNATC 3 cut(s) 72, 458, 1184
BseLI CCNNNNNNNGG 2 cut(s) 207, 1416
BseMI GCAATG 2 cut(s) 212, 1017
BseMII CTCAG 2 cut(s) 1105, 1301
BseNI ACTGG 1 cut(s) 233
BseRI GAGGAG 3 cut(s) 152, 272, 1406
BseSI GKGCMC 1 cut(s) 674
BseXI GCAGC 1 cut(s) 1018
Bsh1236I CGCG 3 cut(s) 117, 333, 1446
Bsh1285I CGRYCG 2 cut(s) 627, 1203
BshFI GGCC 3 cut(s) 188, 483, 921
BshNI GGYRCC 3 cut(s) 669, 902, 1423
BsiEI CGRYCG 2 cut(s) 627, 1203
BsiHKAI GWGCWC 4 cut(s) 291, 1092, 1159, 1409
BsiHKCI CYCGRG 1 cut(s) 284
BslFI GGGAC 1 cut(s) 601
BslI CCNNNNNNNGG 2 cut(s) 207, 1416
BsmAI GTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 601
BsmI GAATGC 1 cut(s) 192
BsnI GGCC 3 cut(s) 188, 483, 921
BsoBI CYCGRG 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 123
Bsp1286I GDGCHC 5 cut(s) 291, 674, 1092, 1159, 1409
Bsp143I GATC 5 cut(s) 67, 228, 1179, 1195, 1200
Bsp68I TCGCGA 1 cut(s) 333
BspACI CCGC 2 cut(s) 384, 1284
BspANI GGCC 3 cut(s) 188, 483, 921
BspCNI CTCAG 2 cut(s) 1104, 1300
BspFNI CGCG 3 cut(s) 117, 333, 1446
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 4 cut(s) 261, 671, 904, 1425
BspMAI CTGCAG 1 cut(s) 1102
BspMI ACCTGC 1 cut(s) 1091
BspPI GGATC 3 cut(s) 75, 223, 1190
BspT104I TTCGAA 1 cut(s) 123
BspT107I GGYRCC 3 cut(s) 669, 902, 1423
BspTI CTTAAG 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 1286
BsrDI GCAATG 2 cut(s) 212, 1017
BsrI ACTGG 1 cut(s) 233
BssECI CCNNGG 3 cut(s) 105, 351, 1410
BssMI GATC 5 cut(s) 67, 228, 1179, 1195, 1200
BssT1I CCWWGG 1 cut(s) 1410
Bst2UI CCWGG 4 cut(s) 65, 202, 264, 907
Bst4CI ACNGT 5 cut(s) 352, 628, 1335, 1345, 1453
BstAFI CTTAAG 1 cut(s) 467
BstBI TTCGAA 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 190, 1328
BstDEI CTNAG 5 cut(s) 238, 866, 1061, 1091, 1287
BstDSI CCRYGG 2 cut(s) 105, 351
BstF5I GGATG 2 cut(s) 651, 721
BstFNI CGCG 3 cut(s) 117, 333, 1446
BstKTI GATC 5 cut(s) 70, 231, 1182, 1198, 1203
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 5 cut(s) 67, 228, 1179, 1195, 1200
BstMCI CGRYCG 2 cut(s) 627, 1203
BstMWI GCNNNNNNNGC 2 cut(s) 185, 1289
BstNI CCWGG 4 cut(s) 65, 202, 264, 907
BstNSI RCATGY 1 cut(s) 1326
BstSCI CCNGG 4 cut(s) 63, 200, 262, 905
BstSFI CTRYAG 1 cut(s) 1098
BstSLI GKGCMC 1 cut(s) 674
BstUI CGCG 3 cut(s) 117, 333, 1446
BstV1I GCAGC 1 cut(s) 1018
BstV2I GAAGAC 3 cut(s) 216, 1104, 1212
BsuI GTATCC 1 cut(s) 1025
BsuRI GGCC 3 cut(s) 188, 483, 921
BtgI CCRYGG 2 cut(s) 105, 351
BtrI CACGTC 2 cut(s) 828, 1384
BtsCI GGATG 2 cut(s) 651, 721
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 3 cut(s) 274, 1159, 1449
BtuMI TCGCGA 1 cut(s) 333
BveI ACCTGC 1 cut(s) 1091
Cac8I GCNNGC 2 cut(s) 190, 1328
CaiI CAGNNNCTG 1 cut(s) 1009
CciI TCATGA 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 186, 482
CseI GACGC 2 cut(s) 317, 1435
Csp6I GTAC 5 cut(s) 99, 1065, 1095, 1128, 1361
CviAII CATG 8 cut(s) 77, 95, 373, 832, 917, 1225, 1323, 1481
CviQI GTAC 5 cut(s) 99, 1065, 1095, 1128, 1361
DdeI CTNAG 5 cut(s) 238, 866, 1061, 1091, 1287
DpnI GATC 5 cut(s) 69, 230, 1181, 1197, 1202
DpnII GATC 5 cut(s) 67, 228, 1179, 1195, 1200
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EciI GGCGGA 1 cut(s) 399
Ecl136II GAGCTC 2 cut(s) 1090, 1407
Eco130I CCWWGG 1 cut(s) 1410
Eco24I GRGCYC 2 cut(s) 1092, 1409
Eco32I GATATC 1 cut(s) 34
Eco53kI GAGCTC 2 cut(s) 1090, 1407
Eco57I CTGAAG 2 cut(s) 1098, 1359
Eco88I CYCGRG 1 cut(s) 284
EcoICRI GAGCTC 2 cut(s) 1090, 1407
EcoRII CCWGG 4 cut(s) 63, 200, 262, 905
EcoRV GATATC 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 1410
EcoT38I GRGCYC 2 cut(s) 1092, 1409
ErhI CCWWGG 1 cut(s) 1410
FaeI CATG 8 cut(s) 80, 98, 376, 835, 920, 1228, 1326, 1484
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FaqI GGGAC 1 cut(s) 601
FatI CATG 8 cut(s) 76, 94, 372, 831, 916, 1224, 1322, 1480
FbaI TGATCA 1 cut(s) 1179
FblI GTMKAC 1 cut(s) 1441
Fnu4HI GCNGC 2 cut(s) 1007, 1284
FokI GGATG 2 cut(s) 638, 728
FriOI GRGCYC 2 cut(s) 1092, 1409
Fsp4HI GCNGC 2 cut(s) 1007, 1284
FspBI CTAG 1 cut(s) 1257
GluI GCNGC 2 cut(s) 1007, 1284
HaeIII GGCC 3 cut(s) 188, 483, 921
HgaI GACGC 2 cut(s) 317, 1435
Hin1II CATG 8 cut(s) 80, 98, 376, 835, 920, 1228, 1326, 1484
HindIII AAGCTT 2 cut(s) 21, 359
HinfI GANTC 7 cut(s) 39, 73, 608, 648, 799, 926, 1208
Hpy166II GTNNAC 5 cut(s) 355, 420, 574, 641, 1442
Hpy188I TCNGA 6 cut(s) 38, 72, 876, 886, 1200, 1306
Hpy188III TCNNGA 8 cut(s) 77, 310, 332, 622, 803, 930, 1257, 1355
Hpy8I GTNNAC 5 cut(s) 355, 420, 574, 641, 1442
Hpy99I CGWCG 3 cut(s) 333, 998, 1208
HpyAV CCTTC 4 cut(s) 167, 605, 684, 893
HpyCH4III ACNGT 5 cut(s) 352, 628, 1335, 1345, 1453
HpyCH4IV ACGT 3 cut(s) 827, 993, 1383
HpyCH4V TGCA 4 cut(s) 530, 969, 1022, 1100
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 1289
HpyF3I CTNAG 5 cut(s) 238, 866, 1061, 1091, 1287
HpySE526I ACGT 3 cut(s) 827, 993, 1383
Hsp92II CATG 8 cut(s) 80, 98, 376, 835, 920, 1228, 1326, 1484
Ksp22I TGATCA 1 cut(s) 1179
Kzo9I GATC 5 cut(s) 67, 228, 1179, 1195, 1200
LmnI GCTCC 4 cut(s) 259, 1162, 1404, 1412
Lsp1109I GCAGC 1 cut(s) 1018
LweI GCATC 5 cut(s) 750, 780, 881, 978, 1360
MaeI CTAG 1 cut(s) 1257
MaeII ACGT 3 cut(s) 827, 993, 1383
MaeIII GTNAC 5 cut(s) 26, 109, 1054, 1335, 1447
MalI GATC 5 cut(s) 69, 230, 1181, 1197, 1202
MbiI CCGCTC 1 cut(s) 1286
MboI GATC 5 cut(s) 67, 228, 1179, 1195, 1200
MboII GAAGA 7 cut(s) 216, 736, 1104, 1212, 1319, 1365, 1472
MhlI GDGCHC 5 cut(s) 291, 674, 1092, 1159, 1409
MluCI AATT 9 cut(s) 125, 293, 506, 550, 660, 786, 1121, 1232, 1262
MluI ACGCGT 1 cut(s) 1444
MmeI TCCRAC 2 cut(s) 679, 1164
MslI CAYNNNNRTG 1 cut(s) 830
MspA1I CMGCKG 1 cut(s) 1009
MspCI CTTAAG 1 cut(s) 467
MspR9I CCNGG 4 cut(s) 65, 202, 264, 907
Mva1269I GAATGC 1 cut(s) 192
MvaI CCWGG 4 cut(s) 65, 202, 264, 907
MvnI CGCG 3 cut(s) 117, 333, 1446
MwoI GCNNNNNNNGC 2 cut(s) 185, 1289
NdeII GATC 5 cut(s) 67, 228, 1179, 1195, 1200
NlaIII CATG 8 cut(s) 80, 98, 376, 835, 920, 1228, 1326, 1484
NlaIV GGNNCC 4 cut(s) 261, 671, 904, 1425
NmuCI GTSAC 3 cut(s) 109, 1054, 1447
NruI TCGCGA 1 cut(s) 333
NspI RCATGY 1 cut(s) 1326
NspV TTCGAA 1 cut(s) 123
PaeR7I CTCGAG 1 cut(s) 284
PagI TCATGA 1 cut(s) 76
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 1200
PctI GAATGC 1 cut(s) 192
PfeI GAWTC 7 cut(s) 39, 73, 608, 648, 799, 926, 1208
PflMI CCANNNNNTGG 1 cut(s) 207
PkrI GCNGC 2 cut(s) 1008, 1285
Ple19I CGATCG 1 cut(s) 1203
Psp124BI GAGCTC 2 cut(s) 1092, 1409
Psp6I CCWGG 4 cut(s) 63, 200, 262, 905
PspGI CCWGG 4 cut(s) 63, 200, 262, 905
PspN4I GGNNCC 4 cut(s) 261, 671, 904, 1425
PspPI GGNCC 2 cut(s) 186, 482
PspXI VCTCGAGB 1 cut(s) 284
PstI CTGCAG 1 cut(s) 1102
PstNI CAGNNNCTG 1 cut(s) 1009
PvuI CGATCG 1 cut(s) 1203
PvuII CAGCTG 1 cut(s) 1009
RruI TCGCGA 1 cut(s) 333
RsaI GTAC 5 cut(s) 100, 1066, 1096, 1129, 1362
RsaNI GTAC 5 cut(s) 99, 1065, 1095, 1128, 1361
RseI CAYNNNNRTG 1 cut(s) 830
SacI GAGCTC 2 cut(s) 1092, 1409
SatI GCNGC 2 cut(s) 1007, 1284
Sau3AI GATC 5 cut(s) 67, 228, 1179, 1195, 1200
Sau96I GGNCC 2 cut(s) 186, 482
ScaI AGTACT 2 cut(s) 1066, 1096
ScrFI CCNGG 4 cut(s) 65, 202, 264, 907
SduI GDGCHC 5 cut(s) 291, 674, 1092, 1159, 1409
SfaNI GCATC 5 cut(s) 750, 780, 881, 978, 1360
SfcI CTRYAG 1 cut(s) 1098
Sfr274I CTCGAG 1 cut(s) 284
SfuI TTCGAA 1 cut(s) 123
SlaI CTCGAG 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 830
SmlI CTYRAG 3 cut(s) 284, 467, 1355
SmoI CTYRAG 3 cut(s) 284, 467, 1355
Sse9I AATT 9 cut(s) 125, 293, 506, 550, 660, 786, 1121, 1232, 1262
SsiI CCGC 2 cut(s) 384, 1284
SspI AATATT 2 cut(s) 809, 847
SspMI CTAG 1 cut(s) 1257
SstI GAGCTC 2 cut(s) 1092, 1409
StyD4I CCNGG 4 cut(s) 63, 200, 262, 905
StyI CCWWGG 1 cut(s) 1410
TaaI ACNGT 5 cut(s) 352, 628, 1335, 1345, 1453
TaiI ACGT 3 cut(s) 830, 996, 1386
TasI AATT 9 cut(s) 125, 293, 506, 550, 660, 786, 1121, 1232, 1262
TatI WGTACW 2 cut(s) 1064, 1094
TauI GCSGC 1 cut(s) 1286
TfiI GAWTC 7 cut(s) 39, 73, 608, 648, 799, 926, 1208
TscAI CASTG 3 cut(s) 274, 1159, 1456
TseFI GTSAC 3 cut(s) 109, 1054, 1447
TseI GCWGC 1 cut(s) 1006
Tsp45I GTSAC 3 cut(s) 109, 1054, 1447
TspDTI ATGAA 6 cut(s) 289, 640, 812, 974, 1473, 1482
TspGWI ACGGA 2 cut(s) 588, 1264
TspRI CASTG 3 cut(s) 274, 1159, 1456
Van91I CCANNNNNTGG 1 cut(s) 207
Vha464I CTTAAG 1 cut(s) 467
XapI RAATTY 2 cut(s) 550, 1232
XbaI TCTAGA 1 cut(s) 1256
XceI RCATGY 1 cut(s) 1326
XcmI CCANNNNNNNNNTGG 2 cut(s) 270, 662
XhoI CTCGAG 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 1441
XspI CTAG 1 cut(s) 1257
ZrmI AGTACT 2 cut(s) 1066, 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.