Rw4G007690

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000687
Physical Location & Seq
Reverse (-)
16538463 .. 16541008
2546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G007690.1

Sequence Viewer

Length: 1563 bp
ATGTATAGGATGACTACTAATGTTCCGTGTTCTAAGAAAAGAACCCACAGCAGCCTGGTCAACAGCGATAGCCATAGTGATAACGAGGACAATCAGGTTAAGAAGCAGGCCAACAGGCAAGAAGATGATCATGAAAAGCAGAACGCCCAGGAATTAGCTGACAATTGTATCTACCAAATCCTCGAATCTCTTTCCACTAGACTTGCTATCCGGGCCACAATTCTTTCCAAGCAGTGGGAACGTGTATGGTCTTTAGTCCCAGTTTTAGATTTTGATGAGAAAGAACCTTATTTTGGCCAATATTATCTTGGTGATTATTATCAACATGAAAAGTTCGTGAAATTTGTCACCATGTGTTTGAAGCGCCGTGAGAATGATAAGCAGTTAGATAAGTTCAGGCTTCGCATGGGATACTGTCAAGAAGACCGTACCGACAGTCGCCTCATGGATAAGTGGTTGAGTTTTGCTGTTAAGAGAAATGTTAAGGAGCTATGTATCAGCCCAATTATGACATCAGTAGAAGGTCATCCAAATGTTGGCTTGACTACAACCTACTGCTTACCTCATATGGTGTTTGTTTCACAGTCTTTAACTATTTTGAACTTGGAGTGTTTGACAATAACGGGCAGTGATGATGATATTCTTGCATTTAGCCTTCCCTCTTTGAAATCTTTGTCCCTAAGATATGTGAAAACGTTTGTGGAGTTGGGATTCTTCAATTTACAGACCTGGTGTCCTTCCCTTGAGAATCTGTCAATAATTGGAGGTTCCAGCTTTGCCAACATTCTTAAAGTTTCAAGTTCCAGCCTCAAGTACTTGGAAGTTATAGATCGCAGAGTGAAAATTCAGGTTGAAGCTGTGAATCTTGAATCTTTCAGATTTGGTTCTAAAACAGCGGTTGGTGAGATGAAGTTTGATTGCCACATAGAAATGGGTTCGTGTGGAGCGCTTAGAAATTTAGACTTCTATAATGGAATTTTTGAAAGTGCATGGTTTGAAGGCTTTGGTTGTAGATTTCCACTGCTTGAGAGTTTGGTCTTATATAAATGCTGCATCAGAAGCACAGGATGTATTAATGCTGTAAGTTTCAATGCTCCAAGCCTACGATCCTTCCGGTTGAGTGGTTGCAGGCAGATCAGCTTTTGCATGAATGTGCCAAAGTTATCTGAGGCCTTCATCATACTGGATGACCACAATGACTCTAAATGGGGCTCTTCTCGTAGAAGAGATTTTCTCTCAAATTTTAATTGCTGCGGAAGAGTTTCTATATATGCTATTGATCCCAAGGCTCTCATCTTTCCAGAGAAGATGAGAAAGAGGAAGTCATGTTGTCCACCATTAGCTAGTGTCAAGCATATGGAGGTAACGGTACAACAGTACCCGATAGGTTATGCAACAGAGGGGATTACAGATTCTGTAAGCTTCAGGGACTCCTTGCGTTGGATGGCACCTGCGTTGGAGACTCTATCAGTAAACAAGAAATGCTGGGAGAATTTCGACCAAGATATTACTTCCAATTTGAGAGTGCCCGTTTCACCTTGGACGGAATTTATCCACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBD

Protein Analysis

520

Amino Acids

59.72

Weight (kDa)

8.13

Isoelectric Point (pI)

54.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 129 - 300 5.7e-09 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 149 - 322 2.2e-11 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1459
Acc36I ACCTGC 1 cut(s) 1459
AccB1I GGYRCC 1 cut(s) 1447
AccB7I CCANNNNNTGG 2 cut(s) 234, 536
AciI CCGC 2 cut(s) 896, 1254
AclI AACGTT 1 cut(s) 695
AclWI GGATC 2 cut(s) 1101, 1274
AcoI YGGCCR 1 cut(s) 295
AcsI RAATTY 7 cut(s) 341, 843, 955, 975, 1240, 1492, 1547
AcuI CTGAAG 1 cut(s) 1408
AdeI CACNNNGTG 1 cut(s) 354
AfaI GTAC 4 cut(s) 430, 815, 1371, 1379
AfeI AGCGCT 1 cut(s) 948
AfiI CCNNNNNNNGG 4 cut(s) 234, 293, 536, 1440
AflIII ACRYGT 1 cut(s) 241
AhdI GACNNNNNGTC 1 cut(s) 732
AjnI CCWGG 3 cut(s) 54, 147, 728
AjuI GAANNNNNNNTTGG 2 cut(s) 276, 308
AluBI AGCT 7 cut(s) 158, 490, 774, 857, 1140, 1343, 1422
AluI AGCT 7 cut(s) 158, 490, 774, 857, 1140, 1343, 1422
Alw26I GTCTC 1 cut(s) 1454
AlwI GGATC 2 cut(s) 1101, 1274
AlwNI CAGNNNCTG 1 cut(s) 1415
Aor51HI AGCGCT 1 cut(s) 948
AoxI GGCC 4 cut(s) 108, 213, 295, 1170
ApeKI GCWGC 3 cut(s) 51, 1050, 1251
ApoI RAATTY 7 cut(s) 341, 843, 955, 975, 1240, 1492, 1547
AseI ATTAAT 1 cut(s) 1074
Asp700I GAANNNNTTC 1 cut(s) 1261
AspLEI GCGC 2 cut(s) 366, 949
AspS9I GGNCC 1 cut(s) 213
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 4 cut(s) 323, 340, 914, 1527
BaeGI GKGCMC 1 cut(s) 1530
BaeI ACNNNNGTAYC 6 cut(s) 151, 184, 1361, 1361, 1394, 1394
BalI TGGCCA 1 cut(s) 297
BanI GGYRCC 1 cut(s) 1447
BanII GRGCYC 1 cut(s) 1214
BbsI GAAGAC 1 cut(s) 429
BbvI GCAGC 3 cut(s) 63, 1037, 1238
BccI CCATC 1 cut(s) 1438
BceAI ACGGC 1 cut(s) 351
BciT130I CCWGG 3 cut(s) 56, 149, 730
BciVI GTATCC 1 cut(s) 404
BclI TGATCA 1 cut(s) 127
BcnI CCSGG 1 cut(s) 212
BcoDI GTCTC 1 cut(s) 1454
BfaI CTAG 2 cut(s) 198, 1344
BfoI RGCGCY 2 cut(s) 367, 950
BfuAI ACCTGC 1 cut(s) 1459
BfuI GTATCC 1 cut(s) 404
BisI GCNGC 3 cut(s) 52, 1051, 1252
BlsI GCNGC 3 cut(s) 53, 1052, 1253
BmcAI AGTACT 1 cut(s) 815
Bme1390I CCNGG 4 cut(s) 56, 149, 212, 730
BmeRI GACNNNNNGTC 1 cut(s) 732
BmgT120I GGNCC 1 cut(s) 213
BmiI GGNNCC 2 cut(s) 769, 1449
BmrFI CCNGG 4 cut(s) 56, 149, 212, 730
BmrI ACTGGG 1 cut(s) 254
BmsI GCATC 1 cut(s) 1062
BmuI ACTGGG 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 429
BplI GAGNNNNNCTC 2 cut(s) 1218, 1250
BpuEI CTTGAG 3 cut(s) 764, 794, 1046
BpuMI CCSGG 1 cut(s) 212
BsaBI GATNNNNATC 1 cut(s) 318
BsaJI CCNNGG 3 cut(s) 147, 1284, 1538
BsaWI WCCGGW 1 cut(s) 1113
Bsc4I CCNNNNNNNGG 4 cut(s) 234, 293, 536, 1440
Bse1I ACTGG 2 cut(s) 260, 1188
Bse8I GATNNNNATC 1 cut(s) 318
BseBI CCWGG 3 cut(s) 56, 149, 730
BseDI CCNNGG 3 cut(s) 147, 1284, 1538
BseGI GGATG 5 cut(s) 15, 526, 1073, 1192, 1449
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 4 cut(s) 234, 293, 536, 1440
BseMII CTCAG 1 cut(s) 1158
BseNI ACTGG 2 cut(s) 260, 1188
BseSI GKGCMC 1 cut(s) 1530
BseXI GCAGC 3 cut(s) 63, 1037, 1238
BseYI CCCAGC 1 cut(s) 1485
BshFI GGCC 4 cut(s) 110, 215, 297, 1172
BshNI GGYRCC 1 cut(s) 1447
BsiSI CCGG 2 cut(s) 211, 1114
BslFI GGGAC 3 cut(s) 242, 661, 1442
BslI CCNNNNNNNGG 4 cut(s) 234, 293, 536, 1440
BsmAI GTCTC 1 cut(s) 1454
BsmFI GGGAC 3 cut(s) 242, 661, 1442
BsnI GGCC 4 cut(s) 110, 215, 297, 1172
Bsp1286I GDGCHC 2 cut(s) 1214, 1530
Bsp143I GATC 5 cut(s) 127, 829, 1106, 1134, 1279
BspACI CCGC 2 cut(s) 896, 1254
BspANI GGCC 4 cut(s) 110, 215, 297, 1172
BspCNI CTCAG 1 cut(s) 1159
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 2 cut(s) 769, 1449
BspMI ACCTGC 1 cut(s) 1459
BspPI GGATC 2 cut(s) 1101, 1274
BspQI GCTCTTC 1 cut(s) 1219
BspT107I GGYRCC 1 cut(s) 1447
BsrI ACTGG 2 cut(s) 260, 1188
BssECI CCNNGG 3 cut(s) 147, 1284, 1538
BssMI GATC 5 cut(s) 127, 829, 1106, 1134, 1279
BssT1I CCWWGG 2 cut(s) 1284, 1538
Bst2UI CCWGG 3 cut(s) 56, 149, 730
Bst4CI ACNGT 6 cut(s) 416, 428, 437, 585, 1369, 1377
Bst6I CTCTTC 3 cut(s) 1219, 1219, 1252
BstC8I GCNNGC 2 cut(s) 108, 1130
BstDEI CTNAG 4 cut(s) 33, 680, 950, 1167
BstF5I GGATG 5 cut(s) 15, 526, 1073, 1192, 1449
BstH2I RGCGCY 2 cut(s) 367, 950
BstHHI GCGC 2 cut(s) 366, 949
BstKTI GATC 5 cut(s) 130, 832, 1109, 1137, 1282
BstMAI GTCTC 1 cut(s) 1454
BstMBI GATC 5 cut(s) 127, 829, 1106, 1134, 1279
BstMWI GCNNNNNNNGC 2 cut(s) 212, 1059
BstNI CCWGG 3 cut(s) 56, 149, 730
BstSCI CCNGG 4 cut(s) 54, 147, 210, 728
BstSLI GKGCMC 1 cut(s) 1530
BstV1I GCAGC 3 cut(s) 63, 1037, 1238
BstV2I GAAGAC 1 cut(s) 429
BsuI GTATCC 1 cut(s) 404
BsuRI GGCC 4 cut(s) 110, 215, 297, 1172
BtsCI GGATG 5 cut(s) 15, 526, 1073, 1192, 1449
BtsI GCAGTG 3 cut(s) 239, 634, 1019
BtsIMutI CAGTG 3 cut(s) 239, 634, 1019
BveI ACCTGC 1 cut(s) 1459
Cac8I GCNNGC 2 cut(s) 108, 1130
CaiI CAGNNNCTG 1 cut(s) 1415
CciI TCATGA 1 cut(s) 130
CfoI GCGC 2 cut(s) 366, 949
Cfr13I GGNCC 1 cut(s) 213
CsiI ACCWGGT 1 cut(s) 728
Csp6I GTAC 4 cut(s) 429, 814, 1370, 1378
CspCI CAANNNNNGTGG 2 cut(s) 184, 219
CviAII CATG 8 cut(s) 131, 326, 352, 406, 445, 990, 1147, 1326
CviQI GTAC 4 cut(s) 429, 814, 1370, 1378
DdeI CTNAG 4 cut(s) 33, 680, 950, 1167
DpnI GATC 5 cut(s) 129, 831, 1108, 1136, 1281
DpnII GATC 5 cut(s) 127, 829, 1106, 1134, 1279
DraIII CACNNNGTG 1 cut(s) 354
DriI GACNNNNNGTC 1 cut(s) 732
EaeI YGGCCR 1 cut(s) 295
Eam1104I CTCTTC 3 cut(s) 1219, 1219, 1252
Eam1105I GACNNNNNGTC 1 cut(s) 732
EarI CTCTTC 3 cut(s) 1219, 1219, 1252
Eco130I CCWWGG 2 cut(s) 1284, 1538
Eco147I AGGCCT 1 cut(s) 1172
Eco24I GRGCYC 1 cut(s) 1214
Eco47III AGCGCT 1 cut(s) 948
Eco57I CTGAAG 1 cut(s) 1408
EcoRII CCWGG 3 cut(s) 54, 147, 728
EcoT14I CCWWGG 2 cut(s) 1284, 1538
EcoT38I GRGCYC 1 cut(s) 1214
ErhI CCWWGG 2 cut(s) 1284, 1538
FaeI CATG 8 cut(s) 134, 329, 355, 409, 448, 993, 1150, 1329
FaqI GGGAC 3 cut(s) 242, 661, 1442
FatI CATG 8 cut(s) 130, 325, 351, 405, 444, 989, 1146, 1325
FauNDI CATATG 2 cut(s) 567, 1356
FbaI TGATCA 1 cut(s) 127
Fnu4HI GCNGC 3 cut(s) 52, 1051, 1252
FokI GGATG 5 cut(s) 22, 513, 1080, 1199, 1456
FriOI GRGCYC 1 cut(s) 1214
Fsp4HI GCNGC 3 cut(s) 52, 1051, 1252
FspBI CTAG 2 cut(s) 198, 1344
GlaI GCGC 2 cut(s) 365, 948
GluI GCNGC 3 cut(s) 52, 1051, 1252
GsaI CCCAGC 1 cut(s) 1489
HaeII RGCGCY 2 cut(s) 367, 950
HaeIII GGCC 4 cut(s) 110, 215, 297, 1172
HapII CCGG 2 cut(s) 211, 1114
HhaI GCGC 2 cut(s) 366, 949
Hin1II CATG 8 cut(s) 134, 329, 355, 409, 448, 993, 1150, 1329
Hin6I GCGC 2 cut(s) 364, 947
HinP1I GCGC 2 cut(s) 364, 947
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HindIII AAGCTT 1 cut(s) 1420
HinfI GANTC 9 cut(s) 185, 711, 748, 862, 869, 1199, 1412, 1430, 1462
HpaII CCGG 2 cut(s) 211, 1114
HphI GGTGA 4 cut(s) 323, 340, 914, 1527
Hpy166II GTNNAC 3 cut(s) 61, 1334, 1474
Hpy188I TCNGA 3 cut(s) 878, 1058, 1168
Hpy188III TCNNGA 5 cut(s) 131, 337, 419, 866, 1301
Hpy8I GTNNAC 3 cut(s) 61, 1334, 1474
HpyAV CCTTC 6 cut(s) 515, 665, 747, 992, 1120, 1183
HpyCH4III ACNGT 6 cut(s) 416, 428, 437, 585, 1369, 1377
HpyCH4IV ACGT 2 cut(s) 241, 695
HpyCH4V TGCA 6 cut(s) 647, 989, 1053, 1128, 1146, 1394
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 1059
HpyF3I CTNAG 4 cut(s) 33, 680, 950, 1167
HpySE526I ACGT 2 cut(s) 241, 695
Hsp92II CATG 8 cut(s) 134, 329, 355, 409, 448, 993, 1150, 1329
HspAI GCGC 2 cut(s) 364, 947
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 5 cut(s) 127, 829, 1106, 1134, 1279
LguI GCTCTTC 1 cut(s) 1219
LmnI GCTCC 3 cut(s) 487, 944, 1099
Lsp1109I GCAGC 3 cut(s) 63, 1037, 1238
LweI GCATC 1 cut(s) 1062
MabI ACCWGGT 1 cut(s) 728
MaeI CTAG 2 cut(s) 198, 1344
MaeII ACGT 2 cut(s) 241, 695
MaeIII GTNAC 2 cut(s) 346, 1363
MalI GATC 5 cut(s) 129, 831, 1108, 1136, 1281
MboI GATC 5 cut(s) 127, 829, 1106, 1134, 1279
MboII GAAGA 7 cut(s) 134, 434, 706, 1206, 1236, 1269, 1318
MfeI CAATTG 1 cut(s) 163
MhlI GDGCHC 2 cut(s) 1214, 1530
MlsI TGGCCA 1 cut(s) 297
MluNI TGGCCA 1 cut(s) 297
MlyI GAGTC 3 cut(s) 1193, 1424, 1456
MmeI TCCRAC 2 cut(s) 1421, 1437
Mox20I TGGCCA 1 cut(s) 297
MroXI GAANNNNTTC 1 cut(s) 1261
MscI TGGCCA 1 cut(s) 297
MseI TTAA 7 cut(s) 99, 471, 483, 590, 789, 1074, 1245
MslI CAYNNNNRTG 3 cut(s) 531, 929, 1151
Msp20I TGGCCA 1 cut(s) 297
MspA1I CMGCKG 1 cut(s) 896
MspI CCGG 2 cut(s) 211, 1114
MspR9I CCNGG 4 cut(s) 56, 149, 212, 730
MunI CAATTG 1 cut(s) 163
MvaI CCWGG 3 cut(s) 56, 149, 730
MwoI GCNNNNNNNGC 2 cut(s) 212, 1059
NciI CCSGG 1 cut(s) 212
NdeI CATATG 2 cut(s) 567, 1356
NdeII GATC 5 cut(s) 127, 829, 1106, 1134, 1279
NlaIII CATG 8 cut(s) 134, 329, 355, 409, 448, 993, 1150, 1329
NlaIV GGNNCC 2 cut(s) 769, 1449
NmuCI GTSAC 1 cut(s) 346
PagI TCATGA 1 cut(s) 130
PaqCI CACCTGC 1 cut(s) 1459
PceI AGGCCT 1 cut(s) 1172
PciSI GCTCTTC 1 cut(s) 1219
PdmI GAANNNNTTC 1 cut(s) 1261
PfeI GAWTC 6 cut(s) 185, 711, 748, 862, 869, 1412
PflMI CCANNNNNTGG 2 cut(s) 234, 536
PkrI GCNGC 3 cut(s) 53, 1052, 1253
PleI GAGTC 3 cut(s) 1193, 1424, 1456
PpsI GAGTC 3 cut(s) 1193, 1424, 1456
PshBI ATTAAT 1 cut(s) 1074
Psp1406I AACGTT 1 cut(s) 695
Psp6I CCWGG 3 cut(s) 54, 147, 728
PspFI CCCAGC 1 cut(s) 1485
PspGI CCWGG 3 cut(s) 54, 147, 728
PspN4I GGNNCC 2 cut(s) 769, 1449
PspPI GGNCC 1 cut(s) 213
PstNI CAGNNNCTG 1 cut(s) 1415
RsaI GTAC 4 cut(s) 430, 815, 1371, 1379
RsaNI GTAC 4 cut(s) 429, 814, 1370, 1378
RseI CAYNNNNRTG 3 cut(s) 531, 929, 1151
SapI GCTCTTC 1 cut(s) 1219
SaqAI TTAA 7 cut(s) 99, 471, 483, 590, 789, 1074, 1245
SatI GCNGC 3 cut(s) 52, 1051, 1252
Sau3AI GATC 5 cut(s) 127, 829, 1106, 1134, 1279
Sau96I GGNCC 1 cut(s) 213
ScaI AGTACT 1 cut(s) 815
SchI GAGTC 3 cut(s) 1193, 1424, 1456
ScrFI CCNGG 4 cut(s) 56, 149, 212, 730
SduI GDGCHC 2 cut(s) 1214, 1530
SexAI ACCWGGT 1 cut(s) 728
SfaNI GCATC 1 cut(s) 1062
SmiMI CAYNNNNRTG 3 cut(s) 531, 929, 1151
SmlI CTYRAG 3 cut(s) 743, 809, 1025
SmoI CTYRAG 3 cut(s) 743, 809, 1025
SseBI AGGCCT 1 cut(s) 1172
SsiI CCGC 2 cut(s) 896, 1254
SspI AATATT 1 cut(s) 302
SspMI CTAG 2 cut(s) 198, 1344
StuI AGGCCT 1 cut(s) 1172
StyD4I CCNGG 4 cut(s) 54, 147, 210, 728
StyI CCWWGG 2 cut(s) 1284, 1538
TaaI ACNGT 6 cut(s) 416, 428, 437, 585, 1369, 1377
TaiI ACGT 2 cut(s) 244, 698
TaqI TCGA 2 cut(s) 183, 1497
TatI WGTACW 1 cut(s) 813
TfiI GAWTC 6 cut(s) 185, 711, 748, 862, 869, 1412
Tru1I TTAA 7 cut(s) 99, 471, 483, 590, 789, 1074, 1245
Tru9I TTAA 7 cut(s) 99, 471, 483, 590, 789, 1074, 1245
TscAI CASTG 3 cut(s) 239, 634, 1026
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 3 cut(s) 51, 1050, 1251
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 5 cut(s) 147, 342, 923, 1163, 1165
TspGWI ACGGA 2 cut(s) 15, 1559
TspRI CASTG 3 cut(s) 239, 634, 1026
Van91I CCANNNNNTGG 2 cut(s) 234, 536
VspI ATTAAT 1 cut(s) 1074
XapI RAATTY 7 cut(s) 341, 843, 955, 975, 1240, 1492, 1547
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
XmnI GAANNNNTTC 1 cut(s) 1261
XspI CTAG 2 cut(s) 198, 1344
ZrmI AGTACT 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.