FvH4_6g44771

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000687
Physical Location & Seq
Reverse (-)
34472107 .. 34474408
2302 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g44771.t1

Sequence Viewer

Length: 1437 bp
ATGTTTATGGAAGGAAAGGAAAATCTTCATGTTTCTTATTATCTTATTATGGTTTATCCCAGGCTGTGGAAAAGGAAAAACGTGAGCAGAAACCGAAAAATCAAAGATGATCATAGACAACCAGACAACAACGGAACATACACAGCCGAGTTACCTAGTAATGTCATCGACCAAATCCTCTCTCTCATTTCCACCAAACGTGCCGTCCGCGCCGGCCTTCTTTCAAAGGAATGGGGACACTTATGGTCTTCGGTTCCTGTGTTAGATTTCGACGAAGACGAGGATGGAAAGCCTGGTGATAAGCTTCAACGAAGAAAGTTCATCAACTTCATAAGAAGGTGTTTGAAGCGCCGTCGAAAAGATAAGTCCAACATAGACAAGTTCAGGCTTCACATGAGGTACTATGGAAGTCTTACTAGCGTAAACCAGTGGTTGAGTTTAGCTGCGGAGAGAAATGTGAAAGAGCTAGAAATATCCTTTATAAGAGAAAAAAACCCCGGGACGAGATCTCACAGACCCTTCTACTTACCACAAACAATTCTCAATAATGCGAAATCCTTGACCACCCTTAAGTTGGAGAGTGTGACCGTGAAGGACAGCATTGACGCCGTAAGTCTTCCTTCTTTGAAAACCATGTCCCTAGAACTAGTGCAATTCAGGAGTTCCTTGTCATTGATGCAGTTAATATCTGGATGCCCTTCTCTAGAGAGAGTGTTGATAACTTCATGCACCGGCCTCTCGGATCTTAAGATTTTGAGTTCGAGCCTCAAATCCTTGGAAGTTGTGAATTGCTACGTGCAGGGTATTAAAGTTGAAGCTGGGAATCTTGAATCTTTGAAGTTTTATGGAAACTTTTGTAGCCGGTATATGTTTCACACCTTCGACATTTTCACATGTCAAAGAGTTAGAAGCATAGAGTTTCGTGCCATATTCTTGACAGACGCATGGTGTAAAGGCTTCAATTTGAGTTTTCCCCTCCTAGAGAGTCTGGCTTTGCATCAATGCCTCTGGGATTACTGGGAATTCGAACAAATCAAAATCTCTAGCCAGAACCTGAAACGTTTCGTATTTTCCGATTCAGGATGCATCAAGCCCTTCTTTTTACTGGACCTTCCTAATTTATCAGAGGCTAGAATCGACCTCCGGTATAGACCTGTGGAGTCTGCTATCCCATGGGATTTCAAATGTCTGAGAAATTGTCTTGAAAATTTCAATACCTCTAGGAAACTAATTCTACATGTTACCTTACCTCAGCTATGTACTTTTCCTGAATGGATCAGGAACAACTACCCCCCACCGTTACCTAGTCTCAAGTATCTTGAGGTAGAAATAAGTGATTCTACAACTGCAGCAACAGATTCTGCCTTGATGGACTCGTTGCATTGGATGGCACCAGTCTCTCAAGTTTCTATCCATTATGAAACAACATCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

479

Amino Acids

55.41

Weight (kDa)

9.11

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 127 - 332 1.3e-16 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 141 - 283 1.1e-16 FBD-associated F-box protein At5g56370, LRR repeats
LRR_2 PF07723 208 - 233 3e-06 Leucine Rich Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 482
AccB1I GGYRCC 1 cut(s) 1390
AccB7I CCANNNNNTGG 1 cut(s) 66
AccII CGCG 1 cut(s) 210
AciI CCGC 2 cut(s) 208, 446
AclI AACGTT 1 cut(s) 1060
AclWI GGATC 2 cut(s) 750, 1283
AcsI RAATTY 2 cut(s) 1022, 1207
AcyI GRCGYC 1 cut(s) 606
AfaI GTAC 2 cut(s) 401, 1261
AfiI CCNNNNNNNGG 2 cut(s) 66, 574
AflII CTTAAG 2 cut(s) 569, 746
AflIII ACRYGT 2 cut(s) 893, 1237
AhlI ACTAGT 1 cut(s) 646
AjnI CCWGG 2 cut(s) 59, 292
AluBI AGCT 5 cut(s) 304, 443, 466, 818, 1255
AluI AGCT 5 cut(s) 304, 443, 466, 818, 1255
Alw26I GTCTC 2 cut(s) 1313, 1402
AlwI GGATC 2 cut(s) 750, 1283
AlwNI CAGNNNCTG 2 cut(s) 1054, 1361
Ama87I CYCGRG 1 cut(s) 497
AoxI GGCC 2 cut(s) 214, 733
ApeKI GCWGC 2 cut(s) 443, 1349
ApoI RAATTY 2 cut(s) 1022, 1207
ArsI GACNNNNNNTTYG 2 cut(s) 189, 221
Asp700I GAANNNNTTC 2 cut(s) 24, 1061
AspLEI GCGC 2 cut(s) 212, 351
AspS9I GGNCC 1 cut(s) 1108
AsuC2I CCSGG 2 cut(s) 498, 499
AsuHPI GGTGA 1 cut(s) 308
AsuII TTCGAA 1 cut(s) 1026
AvaI CYCGRG 1 cut(s) 497
AvaII GGWCC 1 cut(s) 1108
BanI GGYRCC 1 cut(s) 1390
BbsI GAAGAC 3 cut(s) 240, 282, 608
BbvCI CCTCAGC 1 cut(s) 1251
BbvI GCAGC 2 cut(s) 430, 1361
BccI CCATC 3 cut(s) 278, 1363, 1381
BceAI ACGGC 3 cut(s) 188, 336, 593
BciT130I CCWGG 2 cut(s) 61, 294
BclI TGATCA 1 cut(s) 109
BcnI CCSGG 2 cut(s) 498, 499
BcoDI GTCTC 2 cut(s) 1313, 1402
BcuI ACTAGT 1 cut(s) 646
BfmI CTRYAG 1 cut(s) 1347
BfoI RGCGCY 1 cut(s) 352
BfrI CTTAAG 2 cut(s) 569, 746
BglII AGATCT 1 cut(s) 506
BisI GCNGC 2 cut(s) 444, 1350
BlsI GCNGC 2 cut(s) 445, 1351
Bme1390I CCNGG 4 cut(s) 61, 294, 498, 499
Bme18I GGWCC 1 cut(s) 1108
BmeT110I CYCGRG 1 cut(s) 497
BmgT120I GGNCC 1 cut(s) 1108
BmiI GGNNCC 2 cut(s) 255, 1392
BmrFI CCNGG 4 cut(s) 61, 294, 498, 499
BmrI ACTGGG 1 cut(s) 1027
BmsI GCATC 5 cut(s) 666, 683, 1006, 1073, 1095
BmuI ACTGGG 1 cut(s) 1027
BpiI GAAGAC 3 cut(s) 240, 282, 608
Bpu10I CCTNAGC 1 cut(s) 1251
Bpu14I TTCGAA 1 cut(s) 1026
BpuEI CTTGAG 3 cut(s) 1295, 1340, 1386
BpuMI CCSGG 2 cut(s) 498, 499
BsaAI YACGTR 1 cut(s) 796
BsaHI GRCGYC 1 cut(s) 606
BsaJI CCNNGG 5 cut(s) 59, 496, 497, 774, 1172
BsaWI WCCGGW 1 cut(s) 1143
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 574
Bse118I RCCGGY 3 cut(s) 212, 731, 861
Bse1I ACTGG 4 cut(s) 427, 1022, 1110, 1394
BseBI CCWGG 2 cut(s) 61, 294
BseDI CCNNGG 5 cut(s) 59, 496, 497, 774, 1172
BseGI GGATG 4 cut(s) 289, 698, 1088, 1392
BseLI CCNNNNNNNGG 2 cut(s) 66, 574
BseMII CTCAG 2 cut(s) 1181, 1265
BseNI ACTGG 4 cut(s) 427, 1022, 1110, 1394
BseXI GCAGC 2 cut(s) 430, 1361
BseYI CCCAGC 1 cut(s) 818
BsgI GTGCAG 1 cut(s) 818
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 216, 735
BshNI GGYRCC 1 cut(s) 1390
BsiHKCI CYCGRG 1 cut(s) 497
BsiSI CCGG 5 cut(s) 213, 498, 732, 862, 1144
BslFI GGGAC 3 cut(s) 249, 514, 622
BslI CCNNNNNNNGG 2 cut(s) 66, 574
BsmAI GTCTC 2 cut(s) 1313, 1402
BsmFI GGGAC 3 cut(s) 249, 514, 622
BsnI GGCC 2 cut(s) 216, 735
BsoBI CYCGRG 1 cut(s) 497
Bsp119I TTCGAA 1 cut(s) 1026
Bsp143I GATC 4 cut(s) 109, 506, 742, 1275
Bsp19I CCATGG 1 cut(s) 1172
BspACI CCGC 2 cut(s) 208, 446
BspANI GGCC 2 cut(s) 216, 735
BspCNI CTCAG 2 cut(s) 1182, 1264
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 2 cut(s) 255, 1392
BspMAI CTGCAG 1 cut(s) 1351
BspPI GGATC 2 cut(s) 750, 1283
BspT104I TTCGAA 1 cut(s) 1026
BspT107I GGYRCC 1 cut(s) 1390
BspTI CTTAAG 2 cut(s) 569, 746
BsrFI RCCGGY 3 cut(s) 212, 731, 861
BsrI ACTGG 4 cut(s) 427, 1022, 1110, 1394
BssAI RCCGGY 3 cut(s) 212, 731, 861
BssECI CCNNGG 5 cut(s) 59, 496, 497, 774, 1172
BssMI GATC 4 cut(s) 109, 506, 742, 1275
BssNI GRCGYC 1 cut(s) 606
BssT1I CCWWGG 2 cut(s) 774, 1172
Bst2UI CCWGG 2 cut(s) 61, 294
Bst4CI ACNGT 2 cut(s) 589, 1299
BstACI GRCGYC 1 cut(s) 606
BstAFI CTTAAG 2 cut(s) 569, 746
BstBAI YACGTR 1 cut(s) 796
BstBI TTCGAA 1 cut(s) 1026
BstC8I GCNNGC 1 cut(s) 214
BstDEI CTNAG 3 cut(s) 1190, 1251, 1434
BstDSI CCRYGG 1 cut(s) 1172
BstF5I GGATG 4 cut(s) 289, 698, 1088, 1392
BstFNI CGCG 1 cut(s) 210
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 2 cut(s) 212, 351
BstKTI GATC 4 cut(s) 112, 509, 745, 1278
BstMAI GTCTC 2 cut(s) 1313, 1402
BstMBI GATC 4 cut(s) 109, 506, 742, 1275
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 2 cut(s) 61, 294
BstNSI RCATGY 2 cut(s) 897, 1241
BstSCI CCNGG 4 cut(s) 59, 292, 496, 497
BstSFI CTRYAG 1 cut(s) 1347
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 2 cut(s) 430, 1361
BstV2I GAAGAC 3 cut(s) 240, 282, 608
BstX2I RGATCY 2 cut(s) 506, 742
BstYI RGATCY 2 cut(s) 506, 742
BsuRI GGCC 2 cut(s) 216, 735
BtgI CCRYGG 1 cut(s) 1172
BtsCI GGATG 4 cut(s) 289, 698, 1088, 1392
BtsIMutI CAGTG 1 cut(s) 434
Cac8I GCNNGC 1 cut(s) 214
CaiI CAGNNNCTG 2 cut(s) 1054, 1361
CfoI GCGC 2 cut(s) 212, 351
Cfr10I RCCGGY 3 cut(s) 212, 731, 861
Cfr13I GGNCC 1 cut(s) 1108
Cfr9I CCCGGG 1 cut(s) 497
CseI GACGC 2 cut(s) 614, 950
Csp6I GTAC 2 cut(s) 400, 1260
CviAII CATG 8 cut(s) 29, 394, 634, 726, 894, 945, 1173, 1238
CviQI GTAC 2 cut(s) 400, 1260
DdeI CTNAG 3 cut(s) 1190, 1251, 1434
DpnI GATC 4 cut(s) 111, 508, 744, 1277
DpnII GATC 4 cut(s) 109, 506, 742, 1275
Eco130I CCWWGG 2 cut(s) 774, 1172
Eco47I GGWCC 1 cut(s) 1108
Eco88I CYCGRG 1 cut(s) 497
EcoRI GAATTC 1 cut(s) 1022
EcoRII CCWGG 2 cut(s) 59, 292
EcoT14I CCWWGG 2 cut(s) 774, 1172
EcoT22I ATGCAT 1 cut(s) 1088
ErhI CCWWGG 2 cut(s) 774, 1172
FaeI CATG 8 cut(s) 32, 397, 637, 729, 897, 948, 1176, 1241
FalI AAGNNNNNCTT 4 cut(s) 604, 636, 1082, 1114
FaqI GGGAC 3 cut(s) 249, 514, 622
FatI CATG 8 cut(s) 28, 393, 633, 725, 893, 944, 1172, 1237
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 2 cut(s) 444, 1350
FokI GGATG 4 cut(s) 296, 705, 1095, 1399
Fsp4HI GCNGC 2 cut(s) 444, 1350
GlaI GCGC 2 cut(s) 211, 350
GluI GCNGC 2 cut(s) 444, 1350
GsaI CCCAGC 1 cut(s) 822
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 2 cut(s) 216, 735
HapII CCGG 5 cut(s) 213, 498, 732, 862, 1144
HgaI GACGC 2 cut(s) 614, 950
HhaI GCGC 2 cut(s) 212, 351
Hin1I GRCGYC 1 cut(s) 606
Hin1II CATG 8 cut(s) 32, 397, 637, 729, 897, 948, 1176, 1241
Hin6I GCGC 2 cut(s) 210, 349
HinP1I GCGC 2 cut(s) 210, 349
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 9 cut(s) 823, 830, 985, 1076, 1134, 1160, 1337, 1358, 1373
HpaII CCGG 5 cut(s) 213, 498, 732, 862, 1144
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 4 cut(s) 742, 1075, 1126, 1191
Hpy8I GTNNAC 1 cut(s) 424
Hpy99I CGWCG 2 cut(s) 275, 357
HpyCH4III ACNGT 2 cut(s) 589, 1299
HpyCH4IV ACGT 4 cut(s) 81, 199, 795, 1060
HpyCH4V TGCA 8 cut(s) 652, 679, 729, 799, 997, 1086, 1349, 1381
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 3 cut(s) 1190, 1251, 1434
HpySE526I ACGT 4 cut(s) 81, 199, 795, 1060
Hsp92I GRCGYC 1 cut(s) 606
Hsp92II CATG 8 cut(s) 32, 397, 637, 729, 897, 948, 1176, 1241
HspAI GCGC 2 cut(s) 210, 349
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 4 cut(s) 109, 506, 742, 1275
Lsp1109I GCAGC 2 cut(s) 430, 1361
LweI GCATC 5 cut(s) 666, 683, 1006, 1073, 1095
MaeII ACGT 4 cut(s) 81, 199, 795, 1060
MaeIII GTNAC 4 cut(s) 150, 583, 1240, 1299
MalI GATC 4 cut(s) 111, 508, 744, 1277
MboI GATC 4 cut(s) 109, 506, 742, 1275
MboII GAAGA 5 cut(s) 17, 240, 287, 324, 608
MflI RGATCY 2 cut(s) 506, 742
MluCI AATT 9 cut(s) 537, 653, 787, 961, 1022, 1117, 1195, 1207, 1230
MlyI GAGTC 3 cut(s) 994, 1169, 1367
MmeI TCCRAC 2 cut(s) 393, 555
Mph1103I ATGCAT 1 cut(s) 1088
MroNI GCCGGC 1 cut(s) 212
MroXI GAANNNNTTC 2 cut(s) 24, 1061
MseI TTAA 4 cut(s) 570, 683, 747, 807
MspCI CTTAAG 2 cut(s) 569, 746
MspI CCGG 5 cut(s) 213, 498, 732, 862, 1144
MspR9I CCNGG 4 cut(s) 61, 294, 498, 499
MvaI CCWGG 2 cut(s) 61, 294
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 209
NaeI GCCGGC 1 cut(s) 214
NciI CCSGG 2 cut(s) 498, 499
NcoI CCATGG 1 cut(s) 1172
NdeII GATC 4 cut(s) 109, 506, 742, 1275
NgoMIV GCCGGC 1 cut(s) 212
NlaIII CATG 8 cut(s) 32, 397, 637, 729, 897, 948, 1176, 1241
NlaIV GGNNCC 2 cut(s) 255, 1392
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 1 cut(s) 583
NsiI ATGCAT 1 cut(s) 1088
NspI RCATGY 2 cut(s) 897, 1241
NspV TTCGAA 1 cut(s) 1026
PciI ACATGT 2 cut(s) 893, 1237
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 1071
PdiI GCCGGC 1 cut(s) 214
PdmI GAANNNNTTC 2 cut(s) 24, 1061
PfeI GAWTC 6 cut(s) 823, 830, 1076, 1134, 1337, 1358
PflMI CCANNNNNTGG 1 cut(s) 66
PkrI GCNGC 2 cut(s) 445, 1351
PleI GAGTC 3 cut(s) 993, 1168, 1367
PpsI GAGTC 3 cut(s) 993, 1168, 1367
Ppu21I YACGTR 1 cut(s) 796
PscI ACATGT 2 cut(s) 893, 1237
PsiI TTATAA 1 cut(s) 482
Psp1406I AACGTT 1 cut(s) 1060
Psp6I CCWGG 2 cut(s) 59, 292
PspFI CCCAGC 1 cut(s) 818
PspGI CCWGG 2 cut(s) 59, 292
PspN4I GGNNCC 2 cut(s) 255, 1392
PspPI GGNCC 1 cut(s) 1108
PstI CTGCAG 1 cut(s) 1351
PstNI CAGNNNCTG 2 cut(s) 1054, 1361
PsuI RGATCY 2 cut(s) 506, 742
RsaI GTAC 2 cut(s) 401, 1261
RsaNI GTAC 2 cut(s) 400, 1260
SaqAI TTAA 4 cut(s) 570, 683, 747, 807
SatI GCNGC 2 cut(s) 444, 1350
Sau3AI GATC 4 cut(s) 109, 506, 742, 1275
Sau96I GGNCC 1 cut(s) 1108
SchI GAGTC 3 cut(s) 994, 1169, 1367
ScrFI CCNGG 4 cut(s) 61, 294, 498, 499
SfaNI GCATC 5 cut(s) 666, 683, 1006, 1073, 1095
SfcI CTRYAG 1 cut(s) 1347
SfuI TTCGAA 1 cut(s) 1026
SinI GGWCC 1 cut(s) 1108
SmaI CCCGGG 1 cut(s) 499
SmlI CTYRAG 5 cut(s) 569, 746, 1310, 1319, 1401
SmoI CTYRAG 5 cut(s) 569, 746, 1310, 1319, 1401
SpeI ACTAGT 1 cut(s) 646
Sse9I AATT 9 cut(s) 537, 653, 787, 961, 1022, 1117, 1195, 1207, 1230
SsiI CCGC 2 cut(s) 208, 446
StyD4I CCNGG 4 cut(s) 59, 292, 496, 497
StyI CCWWGG 2 cut(s) 774, 1172
TaaI ACNGT 2 cut(s) 589, 1299
TaiI ACGT 4 cut(s) 84, 202, 798, 1063
TaqI TCGA 7 cut(s) 168, 270, 355, 761, 882, 1026, 1137
TasI AATT 9 cut(s) 537, 653, 787, 961, 1022, 1117, 1195, 1207, 1230
TatI WGTACW 1 cut(s) 1259
TfiI GAWTC 6 cut(s) 823, 830, 1076, 1134, 1337, 1358
Tru1I TTAA 4 cut(s) 570, 683, 747, 807
Tru9I TTAA 4 cut(s) 570, 683, 747, 807
TscAI CASTG 1 cut(s) 434
TseFI GTSAC 1 cut(s) 583
TseI GCWGC 2 cut(s) 443, 1349
Tsp45I GTSAC 1 cut(s) 583
TspDTI ATGAA 5 cut(s) 17, 310, 319, 714, 1434
TspGWI ACGGA 1 cut(s) 147
TspMI CCCGGG 1 cut(s) 497
TspRI CASTG 1 cut(s) 434
Van91I CCANNNNNTGG 1 cut(s) 66
Vha464I CTTAAG 2 cut(s) 569, 746
VpaK11BI GGWCC 1 cut(s) 1108
XapI RAATTY 2 cut(s) 1022, 1207
XbaI TCTAGA 1 cut(s) 703
XceI RCATGY 2 cut(s) 897, 1241
XcmI CCANNNNNNNNNTGG 1 cut(s) 571
XmaI CCCGGG 1 cut(s) 497
XmnI GAANNNNTTC 2 cut(s) 24, 1061
Zsp2I ATGCAT 1 cut(s) 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.