Rh2DG582400

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000687
Physical Location & Seq
Reverse (-)
81323214 .. 81324536
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG582400.1

Sequence Viewer

Length: 1323 bp
ATGGTCATGGACACATACACAGCTGAGTTACCCGACAATTTCATCCACCAAGTCCTCTCTCTCCTTTCCACCAAACACGCCGTCCGGGCCGGCATTCTTTCGAAGGAATGGGGGCACTTATGGTCTTCGGTTCCTGTGTTAGATTTCGACGAGGATGAGGATGGGCAGCCATCTGCTGATAACCGTAAGCTTAAACGAAAAATGTTCATCAACTTCATGAGAAGGTGCTTGAAGCGCCGTGGAAAAGATGAGTCTAACATTGAGAAGTTTAGGCTTCACATGAGGTACTTTGGAGGTGCTACTATTGTAAGCAAGTGGTTGAGTTTAGCGGTTGAGAGAAATGTTAAAGAGCTACAAATCTCCTTTATAAGAGGAAGAGAATCCGGGGTGAGATCTTACAGACCCTTCTACTTACCACAAGCAATTCTCAACAATGCAAAATCCTTAACCACCCTAAAGTTGCAGAGTGTGACTGTGAAGGACAGCATTGACCCTATAAACCTTCCTTCTTTGAAAACTTTGTCCCTAAAACAAGTGCAATTCAGGAGTACCTTGTCATTCATGCACTTAATTTCCGGATGCCCTTCTCTTGAGTATTTCTTGTTAACTTCGTGCACCGGCCTCTCGGATCTTAAGATTTTGAGTTTGAGCCTCAAATCCTTGGAAGTTGTTGATTGCGACGTGCGGGGTATTCAAGTTGAAGCTGAGAATCTCGAATCTTTTAAGTTTCATGGAAACGTTTTTAGCCGGTATATGTGGCACATCTTTAACATTTTCTCATGTCAAAGAGTCAGAAGCATAGAGTTCTCTGACATATTCTTGAAAAGCGAATGGTGTGAAGGCTTCAATTCGAGTTTTCCCCTGCTCGAGAGTTTGATTTTGCATAAATGCCACTGGCAGTACTGGGAACACATAAAAATCTTTAGCCAGAACCTGAAACGTTTTGTATTTTCTGATTCAGAACCCATCAAGCCTTTCTTCTCTTTGAACTTTCCAAATTTATCGGAGGCTAGAATCGACCTCTTGTATAGACCAGCAGACCCCCCAGGTACCTTTCCATGGAAATATTTCTACCACCTGAAAAAGTGTCTTGAAAATTTTAATTGCTCTAGAAAAGTAATTCTATATGTTACGAGGGCGGAGCTATGTACTTTTCCAGAACGGATAAGAAACGGATTCACCCCACCATTGCCTAGTCTCAAGCATCTTGATGTAGAGATCGGTACTTCTACAACTGAAGTGACACATTCTGCCTTAATGGACTCCTTGAATTGGATGGCACCAGCGTCTCGAGTTTCGATCCATAAAACAACATTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

440

Amino Acids

51.01

Weight (kDa)

9.1

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 101 - 296 3.7e-17 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 104 - 245 2.8e-15 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
Acc65I GGTACC 1 cut(s) 1049
AccB1I GGYRCC 2 cut(s) 1049, 1279
AccIII TCCGGA 1 cut(s) 575
AciI CCGC 3 cut(s) 329, 685, 1139
AclI AACGTT 2 cut(s) 738, 940
AclWI GGATC 2 cut(s) 636, 1294
AcsI RAATTY 2 cut(s) 997, 1096
AcuI CTGAAG 1 cut(s) 1257
AfaI GTAC 6 cut(s) 287, 550, 902, 1051, 1150, 1225
AfiI CCNNNNNNNGG 2 cut(s) 1059, 1272
AflII CTTAAG 1 cut(s) 632
AgsI TTSAA 9 cut(s) 232, 514, 695, 701, 823, 847, 988, 1094, 1270
AjiI CACGTC 1 cut(s) 682
AjnI CCWGG 1 cut(s) 1045
AluBI AGCT 5 cut(s) 23, 190, 352, 704, 1144
AluI AGCT 5 cut(s) 23, 190, 352, 704, 1144
Alw21I GWGCWC 1 cut(s) 617
Alw26I GTCTC 2 cut(s) 1202, 1293
Alw44I GTGCAC 1 cut(s) 613
AlwI GGATC 2 cut(s) 636, 1294
AlwNI CAGNNNCTG 1 cut(s) 934
Ama87I CYCGRG 2 cut(s) 866, 1290
Aor13HI TCCGGA 1 cut(s) 575
AoxI GGCC 2 cut(s) 87, 619
ApaLI GTGCAC 1 cut(s) 613
ApeKI GCWGC 1 cut(s) 166
ApoI RAATTY 2 cut(s) 997, 1096
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
Asp700I GAANNNNTTC 1 cut(s) 1067
Asp718I GGTACC 1 cut(s) 1049
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 1 cut(s) 87
AsuC2I CCSGG 2 cut(s) 86, 385
AsuHPI GGTGA 2 cut(s) 400, 1171
AsuII TTCGAA 1 cut(s) 101
AvaI CYCGRG 2 cut(s) 866, 1290
BaeGI GKGCMC 2 cut(s) 117, 617
BanI GGYRCC 2 cut(s) 1049, 1279
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 617
BbvI GCAGC 1 cut(s) 178
BccI CCATC 4 cut(s) 155, 178, 974, 1270
BceAI ACGGC 2 cut(s) 65, 222
BciT130I CCWGG 1 cut(s) 1047
BcnI CCSGG 2 cut(s) 86, 385
BcoDI GTCTC 2 cut(s) 1202, 1293
BfaI CTAG 3 cut(s) 1011, 1110, 1194
BfoI RGCGCY 1 cut(s) 238
BfrI CTTAAG 1 cut(s) 632
BglI GCCNNNNNGGC 1 cut(s) 86
BglII AGATCT 1 cut(s) 392
BisI GCNGC 1 cut(s) 167
BlsI GCNGC 1 cut(s) 168
BmcAI AGTACT 1 cut(s) 902
Bme1390I CCNGG 3 cut(s) 86, 385, 1047
BmeT110I CYCGRG 2 cut(s) 866, 1290
BmgBI CACGTC 1 cut(s) 682
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 3 cut(s) 132, 1051, 1281
BmrFI CCNGG 3 cut(s) 86, 385, 1047
BmrI ACTGGG 1 cut(s) 913
BmsI GCATC 2 cut(s) 569, 1213
BmuI ACTGGG 1 cut(s) 913
BpiI GAAGAC 1 cut(s) 117
Bpu14I TTCGAA 1 cut(s) 101
BpuEI CTTGAG 2 cut(s) 611, 1184
BpuMI CCSGG 2 cut(s) 86, 385
BsaJI CCNNGG 5 cut(s) 238, 384, 660, 1045, 1058
BsaWI WCCGGW 1 cut(s) 575
Bsc4I CCNNNNNNNGG 2 cut(s) 1059, 1272
Bse118I RCCGGY 3 cut(s) 89, 617, 747
Bse1I ACTGG 2 cut(s) 899, 908
Bse3DI GCAATG 1 cut(s) 1187
BseAI TCCGGA 1 cut(s) 575
BseBI CCWGG 1 cut(s) 1047
BseDI CCNNGG 5 cut(s) 238, 384, 660, 1045, 1058
BseGI GGATG 5 cut(s) 42, 160, 166, 584, 1281
BseLI CCNNNNNNNGG 2 cut(s) 1059, 1272
BseMI GCAATG 1 cut(s) 1187
BseMII CTCAG 2 cut(s) 15, 696
BseNI ACTGG 2 cut(s) 899, 908
BseSI GKGCMC 2 cut(s) 117, 617
BseXI GCAGC 1 cut(s) 178
BshFI GGCC 2 cut(s) 89, 621
BshNI GGYRCC 2 cut(s) 1049, 1279
BsiHKAI GWGCWC 1 cut(s) 617
BsiHKCI CYCGRG 2 cut(s) 866, 1290
BsiSI CCGG 6 cut(s) 85, 90, 384, 576, 618, 748
BslFI GGGAC 1 cut(s) 508
BslI CCNNNNNNNGG 2 cut(s) 1059, 1272
BsmAI GTCTC 2 cut(s) 1202, 1293
BsmBI CGTCTC 1 cut(s) 1293
BsmFI GGGAC 1 cut(s) 508
BsmI GAATGC 1 cut(s) 93
BsnI GGCC 2 cut(s) 89, 621
BsoBI CYCGRG 2 cut(s) 866, 1290
Bsp119I TTCGAA 1 cut(s) 101
Bsp1286I GDGCHC 2 cut(s) 117, 617
Bsp13I TCCGGA 1 cut(s) 575
Bsp143I GATC 4 cut(s) 392, 628, 1218, 1299
Bsp19I CCATGG 1 cut(s) 1058
BspACI CCGC 3 cut(s) 329, 685, 1139
BspANI GGCC 2 cut(s) 89, 621
BspCNI CTCAG 2 cut(s) 16, 697
BspEI TCCGGA 1 cut(s) 575
BspHI TCATGA 1 cut(s) 216
BspLI GGNNCC 3 cut(s) 132, 1051, 1281
BspPI GGATC 2 cut(s) 636, 1294
BspT104I TTCGAA 1 cut(s) 101
BspT107I GGYRCC 2 cut(s) 1049, 1279
BspTI CTTAAG 1 cut(s) 632
BsrDI GCAATG 1 cut(s) 1187
BsrFI RCCGGY 3 cut(s) 89, 617, 747
BsrI ACTGG 2 cut(s) 899, 908
BssAI RCCGGY 3 cut(s) 89, 617, 747
BssECI CCNNGG 5 cut(s) 238, 384, 660, 1045, 1058
BssMI GATC 4 cut(s) 392, 628, 1218, 1299
BssT1I CCWWGG 2 cut(s) 660, 1058
Bst2UI CCWGG 1 cut(s) 1047
Bst4CI ACNGT 2 cut(s) 185, 475
Bst6I CTCTTC 1 cut(s) 370
BstAFI CTTAAG 1 cut(s) 632
BstBI TTCGAA 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 91
BstDEI CTNAG 3 cut(s) 24, 705, 1320
BstDSI CCRYGG 2 cut(s) 238, 1058
BstF5I GGATG 5 cut(s) 42, 160, 166, 584, 1281
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 4 cut(s) 395, 631, 1221, 1302
BstMAI GTCTC 2 cut(s) 1202, 1293
BstMBI GATC 4 cut(s) 392, 628, 1218, 1299
BstMWI GCNNNNNNNGC 2 cut(s) 86, 234
BstNI CCWGG 1 cut(s) 1047
BstSCI CCNGG 3 cut(s) 84, 383, 1045
BstSLI GKGCMC 2 cut(s) 117, 617
BstV1I GCAGC 1 cut(s) 178
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 2 cut(s) 392, 628
BstYI RGATCY 2 cut(s) 392, 628
BsuRI GGCC 2 cut(s) 89, 621
BtgI CCRYGG 2 cut(s) 238, 1058
BtrI CACGTC 1 cut(s) 682
BtsCI GGATG 5 cut(s) 42, 160, 166, 584, 1281
BtsIMutI CAGTG 1 cut(s) 892
Cac8I GCNNGC 1 cut(s) 91
CaiI CAGNNNCTG 1 cut(s) 934
CciI TCATGA 1 cut(s) 216
CfoI GCGC 1 cut(s) 237
Cfr10I RCCGGY 3 cut(s) 89, 617, 747
Cfr13I GGNCC 1 cut(s) 87
CseI GACGC 1 cut(s) 1275
Csp6I GTAC 6 cut(s) 286, 549, 901, 1050, 1149, 1224
CviAII CATG 7 cut(s) 7, 217, 280, 562, 731, 780, 1059
CviQI GTAC 6 cut(s) 286, 549, 901, 1050, 1149, 1224
DdeI CTNAG 3 cut(s) 24, 705, 1320
DpnI GATC 4 cut(s) 394, 630, 1220, 1301
DpnII GATC 4 cut(s) 392, 628, 1218, 1299
Eam1104I CTCTTC 1 cut(s) 370
EarI CTCTTC 1 cut(s) 370
EciI GGCGGA 1 cut(s) 1154
Eco130I CCWWGG 2 cut(s) 660, 1058
Eco57I CTGAAG 1 cut(s) 1257
Eco88I CYCGRG 2 cut(s) 866, 1290
EcoRII CCWGG 1 cut(s) 1045
EcoT14I CCWWGG 2 cut(s) 660, 1058
ErhI CCWWGG 2 cut(s) 660, 1058
Esp3I CGTCTC 1 cut(s) 1293
FaeI CATG 7 cut(s) 10, 220, 283, 565, 734, 783, 1062
FalI AAGNNNNNCTT 2 cut(s) 962, 994
FaqI GGGAC 1 cut(s) 508
FatI CATG 7 cut(s) 6, 216, 279, 561, 730, 779, 1058
FauI CCCGC 1 cut(s) 678
Fnu4HI GCNGC 1 cut(s) 167
FokI GGATG 5 cut(s) 29, 167, 173, 591, 1288
Fsp4HI GCNGC 1 cut(s) 167
FspBI CTAG 3 cut(s) 1011, 1110, 1194
GlaI GCGC 1 cut(s) 236
GluI GCNGC 1 cut(s) 167
HaeII RGCGCY 1 cut(s) 238
HaeIII GGCC 2 cut(s) 89, 621
HapII CCGG 6 cut(s) 85, 90, 384, 576, 618, 748
HgaI GACGC 1 cut(s) 1275
HhaI GCGC 1 cut(s) 237
Hin1II CATG 7 cut(s) 10, 220, 283, 565, 734, 783, 1062
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 9 cut(s) 251, 380, 709, 716, 789, 956, 1014, 1176, 1262
HpaI GTTAAC 1 cut(s) 606
HpaII CCGG 6 cut(s) 85, 90, 384, 576, 618, 748
HphI GGTGA 2 cut(s) 400, 1171
Hpy166II GTNNAC 2 cut(s) 606, 615
Hpy188I TCNGA 6 cut(s) 628, 794, 811, 955, 961, 1006
Hpy8I GTNNAC 2 cut(s) 606, 615
Hpy99I CGWCG 2 cut(s) 152, 683
HpyAV CCTTC 8 cut(s) 97, 216, 415, 472, 512, 516, 594, 833
HpyCH4III ACNGT 2 cut(s) 185, 475
HpyCH4IV ACGT 3 cut(s) 681, 738, 940
HpyCH4V TGCA 6 cut(s) 437, 463, 538, 565, 615, 883
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 234
HpyF3I CTNAG 3 cut(s) 24, 705, 1320
HpySE526I ACGT 3 cut(s) 681, 738, 940
Hsp92II CATG 7 cut(s) 10, 220, 283, 565, 734, 783, 1062
HspAI GCGC 1 cut(s) 235
Kpn2I TCCGGA 1 cut(s) 575
KpnI GGTACC 1 cut(s) 1053
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
KspAI GTTAAC 1 cut(s) 606
Kzo9I GATC 4 cut(s) 392, 628, 1218, 1299
LmnI GCTCC 1 cut(s) 1141
Lsp1109I GCAGC 1 cut(s) 178
LweI GCATC 2 cut(s) 569, 1213
MaeI CTAG 3 cut(s) 1011, 1110, 1194
MaeII ACGT 3 cut(s) 681, 738, 940
MaeIII GTNAC 4 cut(s) 27, 469, 1129, 1240
MalI GATC 4 cut(s) 394, 630, 1220, 1301
MboI GATC 4 cut(s) 392, 628, 1218, 1299
MboII GAAGA 3 cut(s) 117, 387, 970
MflI RGATCY 2 cut(s) 392, 628
MhlI GDGCHC 2 cut(s) 117, 617
MlyI GAGTC 3 cut(s) 260, 798, 1256
MroI TCCGGA 1 cut(s) 575
MroNI GCCGGC 1 cut(s) 89
MroXI GAANNNNTTC 1 cut(s) 1067
MslI CAYNNNNRTG 1 cut(s) 1209
MspA1I CMGCKG 1 cut(s) 23
MspCI CTTAAG 1 cut(s) 632
MspI CCGG 6 cut(s) 85, 90, 384, 576, 618, 748
MspR9I CCNGG 3 cut(s) 86, 385, 1047
Mva1269I GAATGC 1 cut(s) 93
MvaI CCWGG 1 cut(s) 1047
MwoI GCNNNNNNNGC 2 cut(s) 86, 234
NaeI GCCGGC 1 cut(s) 91
NciI CCSGG 2 cut(s) 86, 385
NcoI CCATGG 1 cut(s) 1058
NdeII GATC 4 cut(s) 392, 628, 1218, 1299
NgoMIV GCCGGC 1 cut(s) 89
NlaIII CATG 7 cut(s) 10, 220, 283, 565, 734, 783, 1062
NlaIV GGNNCC 3 cut(s) 132, 1051, 1281
NmuCI GTSAC 2 cut(s) 469, 1240
NspV TTCGAA 1 cut(s) 101
PaeR7I CTCGAG 2 cut(s) 866, 1290
PagI TCATGA 1 cut(s) 216
PctI GAATGC 1 cut(s) 93
PdiI GCCGGC 1 cut(s) 91
PdmI GAANNNNTTC 1 cut(s) 1067
PfeI GAWTC 6 cut(s) 380, 709, 716, 956, 1014, 1176
PkrI GCNGC 1 cut(s) 168
PleI GAGTC 3 cut(s) 259, 797, 1256
PpsI GAGTC 3 cut(s) 259, 797, 1256
PsiI TTATAA 1 cut(s) 368
Psp1406I AACGTT 2 cut(s) 738, 940
Psp6I CCWGG 1 cut(s) 1045
PspGI CCWGG 1 cut(s) 1045
PspN4I GGNNCC 3 cut(s) 132, 1051, 1281
PspPI GGNCC 1 cut(s) 87
PstNI CAGNNNCTG 1 cut(s) 934
PsuI RGATCY 2 cut(s) 392, 628
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 6 cut(s) 287, 550, 902, 1051, 1150, 1225
RsaNI GTAC 6 cut(s) 286, 549, 901, 1050, 1149, 1224
RseI CAYNNNNRTG 1 cut(s) 1209
SatI GCNGC 1 cut(s) 167
Sau3AI GATC 4 cut(s) 392, 628, 1218, 1299
Sau96I GGNCC 1 cut(s) 87
ScaI AGTACT 1 cut(s) 902
SchI GAGTC 3 cut(s) 260, 798, 1256
ScrFI CCNGG 3 cut(s) 86, 385, 1047
SduI GDGCHC 2 cut(s) 117, 617
SfaNI GCATC 2 cut(s) 569, 1213
Sfr274I CTCGAG 2 cut(s) 866, 1290
SfuI TTCGAA 1 cut(s) 101
SlaI CTCGAG 2 cut(s) 866, 1290
SmiMI CAYNNNNRTG 1 cut(s) 1209
SmlI CTYRAG 5 cut(s) 590, 632, 866, 1199, 1290
SmoI CTYRAG 5 cut(s) 590, 632, 866, 1199, 1290
SsiI CCGC 3 cut(s) 329, 685, 1139
SspI AATATT 1 cut(s) 1067
SspMI CTAG 3 cut(s) 1011, 1110, 1194
StyD4I CCNGG 3 cut(s) 84, 383, 1045
StyI CCWWGG 2 cut(s) 660, 1058
TaaI ACNGT 2 cut(s) 185, 475
TaiI ACGT 3 cut(s) 684, 741, 943
TaqI TCGA 8 cut(s) 101, 147, 714, 851, 867, 1017, 1291, 1298
TatI WGTACW 2 cut(s) 900, 1148
TfiI GAWTC 6 cut(s) 380, 709, 716, 956, 1014, 1176
TscAI CASTG 1 cut(s) 899
TseFI GTSAC 2 cut(s) 469, 1240
TseI GCWGC 1 cut(s) 166
Tsp45I GTSAC 2 cut(s) 469, 1240
TspDTI ATGAA 5 cut(s) 31, 196, 205, 550, 719
TspGWI ACGGA 2 cut(s) 1177, 1188
TspRI CASTG 1 cut(s) 899
Vha464I CTTAAG 1 cut(s) 632
VneI GTGCAC 1 cut(s) 613
XapI RAATTY 2 cut(s) 997, 1096
XbaI TCTAGA 1 cut(s) 1109
XhoI CTCGAG 2 cut(s) 866, 1290
XmnI GAANNNNTTC 1 cut(s) 1067
XspI CTAG 3 cut(s) 1011, 1110, 1194
ZrmI AGTACT 1 cut(s) 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.