pycom09g01880

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000687
Physical Location & Seq
Forward (+)
1408241 .. 1409502
1262 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g01880.1

Sequence Viewer

Length: 1143 bp
ATGAGGTACTCTGGAGGTGCCGATATCATAAATAAGTGGTTGAATTTTGCGGCTGAGAAAAACGTTAAAGAGTTAGAAATCATCCTCAAAAGAAAACGTGGTGACAGAAGCATATCCTACTGCTTACCGTGGGCTATCCTCAATGCACAATTTTTAACCACTCTAAGGTTGGAGAAAGTGAAAATAAAATACAACATTGGTTCTATAAACCTTCCTTCCTTGAAAACTATGACCCTCAAAAATATGGATTTGGGTTACAACACTAACAGGGGATTTGTCCAGTTATTTTCCGGGTGCTCTTCTCTCGAGTCTCTGTCATTAATTTTATGTCCGTTATTTCTTTGCAAAGTTTCAAGCTCAAGCCTCAAATCCTTGCAAGTTATTTGGTGTTCGAAAGTGGTGAGATTCAAAGTTGAAGCTACAAATCTCGAATCATTTAGATTTTGTAGCGGTGATTCAAGTGCTGTGCACATGGATGGACACCTCGACATTGCCTCTTGTCGATCGATTAGAAATCTGGAGTTCTTTGATACAAATTTTTCAGGTGTATGGTTTGAAGATGACCTAAGTCCAAGGTTTCCCCTTCTTGAGAGTTTGACGTTATATAAATGCAGCAACTGGTCATCGGATACAATTCAAGTCAACAATCCGCTTCTGAAACGCCTTGCATTTTGTGGGCGCAATGCCTGTGCTGCAAACGTCAAAATTAATACTCCAAATCTAGCTTTTTTTGCGTTCAGTACTGGATATTTGCCTCCTGATCAATTAAGTGCCTTTGTGTCCAAATTTTCTCTGACAGCTCCCAACTTATTGGAGGCTAACATCAACTTCAACTTCATAGAGAGAGATTTTTCTCCTCTTAGAAAGTTTCTTGAAAGGTGTTTCCCTCGTTTCAGCACAAACAGAACGTTAGATTGCTCAAGGAAAATGTGCCTGTCCCTTAACGAAGCTGAGGCTCTTATTTTTCCAGAAGAAACGAGAAAGACATGTCTTCCGCCATTACCAATTCTCGACCATCTTGAGGTAGCAGTAGATTCTTCAACGGTGGTGAGCGATGATGTCTTGAGGGATTCCTTGCAATGGATGGCACCAAATCTACAGAGTCTATACGTCAATGAAACAAAAATCTTTTCTTCGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBD

Protein Analysis

381

Amino Acids

43.03

Weight (kDa)

8.59

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 9 - 206 2.5e-13 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 11 - 181 1e-13 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 17, 1087
AciI CCGC 4 cut(s) 50, 450, 650, 995
AclI AACGTT 2 cut(s) 63, 908
AcsI RAATTY 3 cut(s) 43, 535, 785
AfaI GTAC 2 cut(s) 8, 742
AfiI CCNNNNNNNGG 3 cut(s) 165, 1021, 1080
AflIII ACRYGT 1 cut(s) 986
AluBI AGCT 5 cut(s) 357, 419, 725, 800, 950
AluI AGCT 5 cut(s) 357, 419, 725, 800, 950
Alw21I GWGCWC 2 cut(s) 299, 471
Alw26I GTCTC 1 cut(s) 315
Alw44I GTGCAC 1 cut(s) 467
AlwNI CAGNNNCTG 1 cut(s) 618
Ama87I CYCGRG 1 cut(s) 305
ApaLI GTGCAC 1 cut(s) 467
ApeKI GCWGC 2 cut(s) 612, 692
ApoI RAATTY 3 cut(s) 43, 535, 785
AseI ATTAAT 2 cut(s) 320, 708
AspLEI GCGC 1 cut(s) 681
AsuC2I CCSGG 1 cut(s) 292
AsuHPI GGTGA 4 cut(s) 113, 412, 464, 1060
AsuII TTCGAA 1 cut(s) 392
AvaI CYCGRG 1 cut(s) 305
BaeGI GKGCMC 1 cut(s) 471
BanI GGYRCC 2 cut(s) 17, 1087
BbsI GAAGAC 1 cut(s) 983
Bbv12I GWGCWC 2 cut(s) 299, 471
BbvCI CCTCAGC 1 cut(s) 951
BbvI GCAGC 2 cut(s) 624, 679
BccI CCATC 3 cut(s) 470, 1023, 1078
BciVI GTATCC 1 cut(s) 622
BclI TGATCA 1 cut(s) 760
BcnI CCSGG 1 cut(s) 292
BcoDI GTCTC 1 cut(s) 315
BfaI CTAG 1 cut(s) 722
BfmI CTRYAG 1 cut(s) 1097
BfuI GTATCC 1 cut(s) 622
BisI GCNGC 3 cut(s) 51, 613, 693
BlsI GCNGC 3 cut(s) 52, 614, 694
BmcAI AGTACT 1 cut(s) 742
Bme1390I CCNGG 1 cut(s) 292
BmeT110I CYCGRG 1 cut(s) 305
BmiI GGNNCC 2 cut(s) 19, 1089
BmrFI CCNGG 1 cut(s) 292
BoxI GACNNNNGTC 1 cut(s) 567
BpiI GAAGAC 1 cut(s) 983
BpmI CTGGAG 2 cut(s) 33, 539
Bpu10I CCTNAGC 1 cut(s) 951
Bpu14I TTCGAA 1 cut(s) 392
BpuEI CTTGAG 5 cut(s) 343, 608, 904, 1040, 1084
BpuMI CCSGG 1 cut(s) 292
Bsa29I ATCGAT 1 cut(s) 506
BsaJI CCNNGG 2 cut(s) 128, 572
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 1021, 1080
Bse1I ACTGG 3 cut(s) 280, 623, 748
Bse3DI GCAATG 3 cut(s) 489, 688, 1085
BseCI ATCGAT 1 cut(s) 506
BseDI CCNNGG 2 cut(s) 128, 572
BseGI GGATG 3 cut(s) 81, 481, 1089
BseLI CCNNNNNNNGG 3 cut(s) 165, 1021, 1080
BseMI GCAATG 3 cut(s) 489, 688, 1085
BseMII CTCAG 2 cut(s) 45, 942
BseNI ACTGG 3 cut(s) 280, 623, 748
BseRI GAGGAG 1 cut(s) 846
BseSI GKGCMC 1 cut(s) 471
BseXI GCAGC 2 cut(s) 624, 679
Bsh1285I CGRYCG 1 cut(s) 506
BshNI GGYRCC 2 cut(s) 17, 1087
BshVI ATCGAT 1 cut(s) 506
BsiEI CGRYCG 1 cut(s) 506
BsiHKAI GWGCWC 2 cut(s) 299, 471
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 1 cut(s) 291
BslFI GGGAC 1 cut(s) 922
BslI CCNNNNNNNGG 3 cut(s) 165, 1021, 1080
BsmAI GTCTC 1 cut(s) 315
BsmFI GGGAC 1 cut(s) 922
BsoBI CYCGRG 1 cut(s) 305
Bsp119I TTCGAA 1 cut(s) 392
Bsp1286I GDGCHC 2 cut(s) 299, 471
Bsp143I GATC 2 cut(s) 503, 760
BspACI CCGC 4 cut(s) 50, 450, 650, 995
BspCNI CTCAG 2 cut(s) 46, 943
BspDI ATCGAT 1 cut(s) 506
BspLI GGNNCC 2 cut(s) 19, 1089
BspQI GCTCTTC 1 cut(s) 304
BspT104I TTCGAA 1 cut(s) 392
BspT107I GGYRCC 2 cut(s) 17, 1087
BsrDI GCAATG 3 cut(s) 489, 688, 1085
BsrI ACTGG 3 cut(s) 280, 623, 748
BssECI CCNNGG 2 cut(s) 128, 572
BssMI GATC 2 cut(s) 503, 760
BssT1I CCWWGG 1 cut(s) 572
Bst4CI ACNGT 2 cut(s) 129, 1045
Bst6I CTCTTC 1 cut(s) 304
BstBI TTCGAA 1 cut(s) 392
BstDEI CTNAG 5 cut(s) 54, 164, 566, 860, 951
BstDSI CCRYGG 1 cut(s) 128
BstF5I GGATG 3 cut(s) 81, 481, 1089
BstHHI GCGC 1 cut(s) 681
BstKTI GATC 2 cut(s) 506, 763
BstMAI GTCTC 1 cut(s) 315
BstMBI GATC 2 cut(s) 503, 760
BstMCI CGRYCG 1 cut(s) 506
BstMWI GCNNNNNNNGC 2 cut(s) 692, 731
BstNSI RCATGY 1 cut(s) 990
BstPAI GACNNNNGTC 1 cut(s) 567
BstSCI CCNGG 1 cut(s) 290
BstSFI CTRYAG 1 cut(s) 1097
BstSLI GKGCMC 1 cut(s) 471
BstV1I GCAGC 2 cut(s) 624, 679
BstV2I GAAGAC 1 cut(s) 983
BstXI CCANNNNNNTGG 1 cut(s) 811
Bsu15I ATCGAT 1 cut(s) 506
BsuI GTATCC 1 cut(s) 622
BsuTUI ATCGAT 1 cut(s) 506
BtgI CCRYGG 1 cut(s) 128
BtgZI GCGATG 1 cut(s) 1068
BtsCI GGATG 3 cut(s) 81, 481, 1089
CaiI CAGNNNCTG 1 cut(s) 618
CfoI GCGC 1 cut(s) 681
ClaI ATCGAT 1 cut(s) 506
Csp6I GTAC 2 cut(s) 7, 741
CviAII CATG 2 cut(s) 472, 987
CviQI GTAC 2 cut(s) 7, 741
DdeI CTNAG 5 cut(s) 54, 164, 566, 860, 951
DpnI GATC 2 cut(s) 505, 762
DpnII GATC 2 cut(s) 503, 760
Eam1104I CTCTTC 1 cut(s) 304
EarI CTCTTC 1 cut(s) 304
EciI GGCGGA 1 cut(s) 984
Eco130I CCWWGG 1 cut(s) 572
Eco32I GATATC 1 cut(s) 25
Eco88I CYCGRG 1 cut(s) 305
EcoRV GATATC 1 cut(s) 25
EcoT14I CCWWGG 1 cut(s) 572
ErhI CCWWGG 1 cut(s) 572
FaeI CATG 2 cut(s) 475, 990
FaqI GGGAC 1 cut(s) 922
FatI CATG 2 cut(s) 471, 986
FbaI TGATCA 1 cut(s) 760
Fnu4HI GCNGC 3 cut(s) 51, 613, 693
FokI GGATG 3 cut(s) 68, 488, 1096
Fsp4HI GCNGC 3 cut(s) 51, 613, 693
FspBI CTAG 1 cut(s) 722
GlaI GCGC 1 cut(s) 680
GluI GCNGC 3 cut(s) 51, 613, 693
GsuI CTGGAG 2 cut(s) 33, 539
HapII CCGG 1 cut(s) 291
HhaI GCGC 1 cut(s) 681
Hin1II CATG 2 cut(s) 475, 990
Hin6I GCGC 1 cut(s) 679
HinP1I GCGC 1 cut(s) 679
HincII GTYRAC 1 cut(s) 643
HindII GTYRAC 1 cut(s) 643
HinfI GANTC 7 cut(s) 308, 405, 431, 455, 1034, 1070, 1102
HpaII CCGG 1 cut(s) 291
HphI GGTGA 4 cut(s) 113, 412, 464, 1060
Hpy166II GTNNAC 2 cut(s) 469, 643
Hpy188I TCNGA 3 cut(s) 628, 657, 795
Hpy8I GTNNAC 2 cut(s) 469, 643
HpyAV CCTTC 3 cut(s) 221, 225, 593
HpyCH4III ACNGT 2 cut(s) 129, 1045
HpyCH4IV ACGT 6 cut(s) 63, 97, 599, 699, 908, 1110
HpyCH4V TGCA 8 cut(s) 146, 345, 376, 469, 612, 668, 695, 1078
HpyF10VI GCNNNNNNNGC 2 cut(s) 692, 731
HpyF3I CTNAG 5 cut(s) 54, 164, 566, 860, 951
HpySE526I ACGT 6 cut(s) 63, 97, 599, 699, 908, 1110
Hsp92II CATG 2 cut(s) 475, 990
HspAI GCGC 1 cut(s) 679
Ksp22I TGATCA 1 cut(s) 760
Kzo9I GATC 2 cut(s) 503, 760
LguI GCTCTTC 1 cut(s) 304
LmnI GCTCC 1 cut(s) 805
Lsp1109I GCAGC 2 cut(s) 624, 679
MaeI CTAG 1 cut(s) 722
MaeII ACGT 6 cut(s) 63, 97, 599, 699, 908, 1110
MaeIII GTNAC 2 cut(s) 101, 254
MalI GATC 2 cut(s) 505, 762
MboI GATC 2 cut(s) 503, 760
MboII GAAGA 6 cut(s) 291, 569, 983, 983, 1029, 1125
MhlI GDGCHC 2 cut(s) 299, 471
MluCI AATT 9 cut(s) 43, 149, 321, 535, 633, 705, 764, 785, 1005
MlyI GAGTC 2 cut(s) 317, 1111
MmeI TCCRAC 1 cut(s) 150
MseI TTAA 6 cut(s) 66, 155, 320, 708, 767, 942
MslI CAYNNNNRTG 1 cut(s) 474
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 1 cut(s) 292
MwoI GCNNNNNNNGC 2 cut(s) 692, 731
NciI CCSGG 1 cut(s) 292
NdeII GATC 2 cut(s) 503, 760
NlaIII CATG 2 cut(s) 475, 990
NlaIV GGNNCC 2 cut(s) 19, 1089
NmuCI GTSAC 1 cut(s) 101
NspI RCATGY 1 cut(s) 990
NspV TTCGAA 1 cut(s) 392
PaeR7I CTCGAG 1 cut(s) 305
PciI ACATGT 1 cut(s) 986
PciSI GCTCTTC 1 cut(s) 304
PfeI GAWTC 5 cut(s) 405, 431, 455, 1034, 1070
PkrI GCNGC 3 cut(s) 52, 614, 694
Ple19I CGATCG 1 cut(s) 506
PleI GAGTC 2 cut(s) 316, 1110
PpsI GAGTC 2 cut(s) 316, 1110
PscI ACATGT 1 cut(s) 986
PshAI GACNNNNGTC 1 cut(s) 567
PshBI ATTAAT 2 cut(s) 320, 708
Psp1406I AACGTT 2 cut(s) 63, 908
PspN4I GGNNCC 2 cut(s) 19, 1089
PstNI CAGNNNCTG 1 cut(s) 618
PvuI CGATCG 1 cut(s) 506
RsaI GTAC 2 cut(s) 8, 742
RsaNI GTAC 2 cut(s) 7, 741
RseI CAYNNNNRTG 1 cut(s) 474
SapI GCTCTTC 1 cut(s) 304
SaqAI TTAA 6 cut(s) 66, 155, 320, 708, 767, 942
SatI GCNGC 3 cut(s) 51, 613, 693
Sau3AI GATC 2 cut(s) 503, 760
ScaI AGTACT 1 cut(s) 742
SchI GAGTC 2 cut(s) 317, 1111
ScrFI CCNGG 1 cut(s) 292
SduI GDGCHC 2 cut(s) 299, 471
SfcI CTRYAG 1 cut(s) 1097
Sfr274I CTCGAG 1 cut(s) 305
SfuI TTCGAA 1 cut(s) 392
SlaI CTCGAG 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 6 cut(s) 305, 358, 587, 919, 1019, 1063
SmoI CTYRAG 6 cut(s) 305, 358, 587, 919, 1019, 1063
Sse9I AATT 9 cut(s) 43, 149, 321, 535, 633, 705, 764, 785, 1005
SsiI CCGC 4 cut(s) 50, 450, 650, 995
SspMI CTAG 1 cut(s) 722
StyD4I CCNGG 1 cut(s) 290
StyI CCWWGG 1 cut(s) 572
TaaI ACNGT 2 cut(s) 129, 1045
TaiI ACGT 6 cut(s) 66, 100, 602, 702, 911, 1113
TaqI TCGA 7 cut(s) 306, 392, 429, 486, 502, 506, 1011
TasI AATT 9 cut(s) 43, 149, 321, 535, 633, 705, 764, 785, 1005
TatI WGTACW 1 cut(s) 740
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 5 cut(s) 405, 431, 455, 1034, 1070
Tru1I TTAA 6 cut(s) 66, 155, 320, 708, 767, 942
Tru9I TTAA 6 cut(s) 66, 155, 320, 708, 767, 942
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 2 cut(s) 612, 692
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 2 cut(s) 826, 1131
TspGWI ACGGA 1 cut(s) 321
VneI GTGCAC 1 cut(s) 467
VspI ATTAAT 2 cut(s) 320, 708
XapI RAATTY 3 cut(s) 43, 535, 785
XceI RCATGY 1 cut(s) 990
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XhoI CTCGAG 1 cut(s) 305
XspI CTAG 1 cut(s) 722
ZrmI AGTACT 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.