pycom09g01850

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000687
Physical Location & Seq
Forward (+)
1402634 .. 1403884
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g01850.1

Sequence Viewer

Length: 1251 bp
ATGATTAAGAGAAAAGCCGAAGCTTGTAACGATATCAGAAACATAAATAAAAAGACCAAAAGCCAGGATCAGAATCATGACATTCAAAACCAACATGGTACGGCCGTGGTCACACGCGAGTTCGAATTACCCGACCTTGTTGTCTCCCAAGTTCTCCTTCTCCTCCCCACCAAACTCGCTGTTCGGGCCAGCATTCTTTCCAGGCAATGGGAACGGGTGTGGTCTTCGATCCCAGTCTTAGTTTTCAATGAGTATGAGGAGCCAGGCAGTGATTGGCACACCCTCGAGCACAAATTGTTCATTGACTTCTTGAAAAAGTGCTTGAAGTGTCGTGAGAAAGATAAATACACCGTGGACAAGCTTACGCTTCACATGAGGTATGGTGGAGGGAGAGGAAGCACGGCTATCATAGATAAGTGGACGAGTTTTGCTGTTGAGAGAAACATTAAAGAGATAGACATCATTCTTGAGAGAAAACTTGGCCCTGATAAAAATGCCATATACAAATTATCGCAAACCATTCTCAATGCAAAGTTTTTAACTACACTAAATTTAGAGAATGTGAGAATAGTGGACAATATGAGTCGTATAAACCTTCCGTCTTTGGAATCTATGTCCCTCACGACCGTAGTGTTGAGTAAACTTGGATTCATCCAACTAATTTCTGGGTGCCCTTCCATCGAGTATTTGTCATTAAATCAATGTAGATTAAAGGATGATGTGAAGATTTCGAGTTTGAGCATCAAATCCTTGAAAATTGTTGAATGTAGAATTGTCGGAATGGAAATTGAAGCCATGAATCTCGAATATTTTTTAAGTTGCTGCACGTTCATGAGCAAAATCAATATTGCCTCTTGTCGAACACTTAGAAATCTGAAATTCTCTGATAGATGCTTGGAAGGTGCCTGGTTTGAACATGGCCTTGATTCAAGATTTCCACTCTTGGAGAGTTTAACAATGCATAGCTGCATCATCTCATCGTCAATCATTAACGTCGATAATCAGCAGCTGAAACACATTGCACTTCATGGATACGACAAAAATGTAGCAAAGGTCACGCTTAGTACTCCAAATCTACTTTCTTTCGAGCTCAGTACTGCAGGTTATTTGTCTTCAGCTGAATTCAGTAATTTGGTGTCCAACTTTTCTATCAGTGCTCCAAAGTTGTTAGAGGCTACGATCGATCATCAACTTCGATCCAATTGTCGATTCTTATTCGTCTCCTATGAGAAAATTTCTTGGGAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_2

Protein Analysis

417

Amino Acids

47.43

Weight (kDa)

8.61

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 120 - 271 2.3e-10 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 137 - 272 5.9e-11 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 140
Acc36I ACCTGC 1 cut(s) 1091
AccB1I GGYRCC 2 cut(s) 669, 902
AccB7I CCANNNNNTGG 1 cut(s) 207
AccII CGCG 1 cut(s) 117
AclWI GGATC 3 cut(s) 75, 223, 1191
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 4 cut(s) 550, 878, 1121, 1233
AcuI CTGAAG 1 cut(s) 1098
AfaI GTAC 3 cut(s) 100, 1066, 1096
AfiI CCNNNNNNNGG 1 cut(s) 207
AgsI TTSAA 9 cut(s) 86, 247, 313, 325, 754, 764, 791, 914, 930
AjnI CCWGG 4 cut(s) 63, 200, 262, 905
AjuI GAANNNNNNNTTGG 6 cut(s) 141, 173, 1063, 1095, 1193, 1225
AluBI AGCT 6 cut(s) 23, 361, 966, 1009, 1090, 1118
AluI AGCT 6 cut(s) 23, 361, 966, 1009, 1090, 1118
Alw21I GWGCWC 3 cut(s) 291, 1092, 1159
Alw26I GTCTC 2 cut(s) 148, 1225
AlwI GGATC 3 cut(s) 75, 223, 1191
AlwNI CAGNNNCTG 1 cut(s) 1009
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 4 cut(s) 102, 186, 481, 919
ApeKI GCWGC 3 cut(s) 822, 966, 1006
ApoI RAATTY 4 cut(s) 550, 878, 1121, 1233
AspS9I GGNCC 2 cut(s) 186, 482
AsuII TTCGAA 1 cut(s) 123
AvaI CYCGRG 1 cut(s) 284
BaeGI GKGCMC 1 cut(s) 674
BaeI ACNNNNGTAYC 2 cut(s) 90, 123
BanI GGYRCC 2 cut(s) 669, 902
BanII GRGCYC 1 cut(s) 1092
BbsI GAAGAC 2 cut(s) 216, 1104
Bbv12I GWGCWC 3 cut(s) 291, 1092, 1159
BbvI GCAGC 3 cut(s) 809, 953, 1018
BccI CCATC 1 cut(s) 686
BceAI ACGGC 3 cut(s) 89, 117, 417
BciT130I CCWGG 4 cut(s) 65, 202, 264, 907
BciVI GTATCC 1 cut(s) 1025
BcoDI GTCTC 2 cut(s) 148, 1225
BfmI CTRYAG 1 cut(s) 1098
BfuAI ACCTGC 1 cut(s) 1091
BfuI GTATCC 1 cut(s) 1025
BisI GCNGC 3 cut(s) 823, 967, 1007
BlsI GCNGC 3 cut(s) 824, 968, 1008
BmcAI AGTACT 2 cut(s) 1066, 1096
Bme1390I CCNGG 4 cut(s) 65, 202, 264, 907
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 2 cut(s) 186, 482
BmiI GGNNCC 3 cut(s) 261, 671, 904
BmrFI CCNGG 4 cut(s) 65, 202, 264, 907
BmrI ACTGGG 1 cut(s) 227
BmsI GCATC 3 cut(s) 750, 881, 978
BmuI ACTGGG 1 cut(s) 227
BpiI GAAGAC 2 cut(s) 216, 1104
Bpu14I TTCGAA 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 488
Bsa29I ATCGAT 1 cut(s) 1182
BsaBI GATNNNNATC 2 cut(s) 72, 458
BsaJI CCNNGG 2 cut(s) 105, 351
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse1I ACTGG 1 cut(s) 233
Bse3DI GCAATG 2 cut(s) 212, 1017
Bse8I GATNNNNATC 2 cut(s) 72, 458
BseBI CCWGG 4 cut(s) 65, 202, 264, 907
BseCI ATCGAT 1 cut(s) 1182
BseDI CCNNGG 2 cut(s) 105, 351
BseGI GGATG 2 cut(s) 651, 721
BseJI GATNNNNATC 2 cut(s) 72, 458
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMI GCAATG 2 cut(s) 212, 1017
BseMII CTCAG 1 cut(s) 1105
BseNI ACTGG 1 cut(s) 233
BseRI GAGGAG 2 cut(s) 152, 272
BseSI GKGCMC 1 cut(s) 674
BseX3I CGGCCG 1 cut(s) 102
BseXI GCAGC 3 cut(s) 809, 953, 1018
BsgI GTGCAG 1 cut(s) 808
Bsh1236I CGCG 1 cut(s) 117
Bsh1285I CGRYCG 3 cut(s) 105, 627, 1182
BshFI GGCC 4 cut(s) 104, 188, 483, 921
BshNI GGYRCC 2 cut(s) 669, 902
BshVI ATCGAT 1 cut(s) 1182
BsiEI CGRYCG 3 cut(s) 105, 627, 1182
BsiHKAI GWGCWC 3 cut(s) 291, 1092, 1159
BsiHKCI CYCGRG 1 cut(s) 284
BslFI GGGAC 1 cut(s) 601
BslI CCNNNNNNNGG 1 cut(s) 207
BsmAI GTCTC 2 cut(s) 148, 1225
BsmBI CGTCTC 1 cut(s) 1225
BsmFI GGGAC 1 cut(s) 601
BsmI GAATGC 1 cut(s) 192
BsnI GGCC 4 cut(s) 104, 188, 483, 921
BsoBI CYCGRG 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 123
Bsp1286I GDGCHC 4 cut(s) 291, 674, 1092, 1159
Bsp143I GATC 5 cut(s) 67, 228, 1179, 1183, 1196
BspANI GGCC 4 cut(s) 104, 188, 483, 921
BspCNI CTCAG 1 cut(s) 1104
BspDI ATCGAT 1 cut(s) 1182
BspFNI CGCG 1 cut(s) 117
BspHI TCATGA 2 cut(s) 76, 831
BspLI GGNNCC 3 cut(s) 261, 671, 904
BspMAI CTGCAG 1 cut(s) 1102
BspMI ACCTGC 1 cut(s) 1091
BspPI GGATC 3 cut(s) 75, 223, 1191
BspT104I TTCGAA 1 cut(s) 123
BspT107I GGYRCC 2 cut(s) 669, 902
BsrDI GCAATG 2 cut(s) 212, 1017
BsrI ACTGG 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 105, 351
BssMI GATC 5 cut(s) 67, 228, 1179, 1183, 1196
Bst2UI CCWGG 4 cut(s) 65, 202, 264, 907
Bst4CI ACNGT 2 cut(s) 352, 628
BstBI TTCGAA 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 190
BstDEI CTNAG 4 cut(s) 238, 866, 1061, 1091
BstDSI CCRYGG 2 cut(s) 105, 351
BstF5I GGATG 2 cut(s) 651, 721
BstFNI CGCG 1 cut(s) 117
BstKTI GATC 5 cut(s) 70, 231, 1182, 1186, 1199
BstMAI GTCTC 2 cut(s) 148, 1225
BstMBI GATC 5 cut(s) 67, 228, 1179, 1183, 1196
BstMCI CGRYCG 3 cut(s) 105, 627, 1182
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstNI CCWGG 4 cut(s) 65, 202, 264, 907
BstSCI CCNGG 4 cut(s) 63, 200, 262, 905
BstSFI CTRYAG 1 cut(s) 1098
BstSLI GKGCMC 1 cut(s) 674
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 3 cut(s) 809, 953, 1018
BstV2I GAAGAC 2 cut(s) 216, 1104
BstZI CGGCCG 1 cut(s) 102
Bsu15I ATCGAT 1 cut(s) 1182
BsuI GTATCC 1 cut(s) 1025
BsuRI GGCC 4 cut(s) 104, 188, 483, 921
BsuTUI ATCGAT 1 cut(s) 1182
BtgI CCRYGG 2 cut(s) 105, 351
BtsCI GGATG 2 cut(s) 651, 721
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 2 cut(s) 274, 1159
BveI ACCTGC 1 cut(s) 1091
Cac8I GCNNGC 1 cut(s) 190
CaiI CAGNNNCTG 1 cut(s) 1009
CciI TCATGA 2 cut(s) 76, 831
Cfr13I GGNCC 2 cut(s) 186, 482
ClaI ATCGAT 1 cut(s) 1182
Csp6I GTAC 3 cut(s) 99, 1065, 1095
CviAII CATG 7 cut(s) 77, 95, 373, 796, 832, 917, 1028
CviQI GTAC 3 cut(s) 99, 1065, 1095
DdeI CTNAG 4 cut(s) 238, 866, 1061, 1091
DpnI GATC 5 cut(s) 69, 230, 1181, 1185, 1198
DpnII GATC 5 cut(s) 67, 228, 1179, 1183, 1196
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 1 cut(s) 102
EagI CGGCCG 1 cut(s) 102
Ecl136II GAGCTC 1 cut(s) 1090
EclXI CGGCCG 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 1092
Eco32I GATATC 1 cut(s) 34
Eco52I CGGCCG 1 cut(s) 102
Eco53kI GAGCTC 1 cut(s) 1090
Eco57I CTGAAG 1 cut(s) 1098
Eco88I CYCGRG 1 cut(s) 284
EcoICRI GAGCTC 1 cut(s) 1090
EcoRI GAATTC 1 cut(s) 1121
EcoRII CCWGG 4 cut(s) 63, 200, 262, 905
EcoRV GATATC 1 cut(s) 34
EcoT22I ATGCAT 1 cut(s) 963
EcoT38I GRGCYC 1 cut(s) 1092
Esp3I CGTCTC 1 cut(s) 1225
FaeI CATG 7 cut(s) 80, 98, 376, 799, 835, 920, 1031
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FaqI GGGAC 1 cut(s) 601
FatI CATG 7 cut(s) 76, 94, 372, 795, 831, 916, 1027
Fnu4HI GCNGC 3 cut(s) 823, 967, 1007
FokI GGATG 2 cut(s) 638, 728
FriOI GRGCYC 1 cut(s) 1092
Fsp4HI GCNGC 3 cut(s) 823, 967, 1007
GluI GCNGC 3 cut(s) 823, 967, 1007
HaeIII GGCC 4 cut(s) 104, 188, 483, 921
Hin1II CATG 7 cut(s) 80, 98, 376, 799, 835, 920, 1031
HindIII AAGCTT 2 cut(s) 21, 359
HinfI GANTC 7 cut(s) 73, 583, 608, 648, 799, 926, 1209
Hpy166II GTNNAC 4 cut(s) 355, 420, 574, 641
Hpy188I TCNGA 5 cut(s) 38, 72, 779, 876, 886
Hpy188III TCNNGA 8 cut(s) 77, 310, 332, 467, 622, 803, 832, 930
Hpy8I GTNNAC 4 cut(s) 355, 420, 574, 641
Hpy99I CGWCG 1 cut(s) 998
HpyAV CCTTC 4 cut(s) 167, 605, 684, 893
HpyCH4III ACNGT 2 cut(s) 352, 628
HpyCH4IV ACGT 2 cut(s) 827, 993
HpyCH4V TGCA 6 cut(s) 530, 825, 961, 969, 1022, 1100
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpyF3I CTNAG 4 cut(s) 238, 866, 1061, 1091
HpySE526I ACGT 2 cut(s) 827, 993
Hsp92II CATG 7 cut(s) 80, 98, 376, 799, 835, 920, 1031
Kzo9I GATC 5 cut(s) 67, 228, 1179, 1183, 1196
LmnI GCTCC 2 cut(s) 259, 1162
Lsp1109I GCAGC 3 cut(s) 809, 953, 1018
LweI GCATC 3 cut(s) 750, 881, 978
MaeII ACGT 2 cut(s) 827, 993
MaeIII GTNAC 3 cut(s) 26, 109, 1054
MalI GATC 5 cut(s) 69, 230, 1181, 1185, 1198
MboI GATC 5 cut(s) 67, 228, 1179, 1183, 1196
MboII GAAGA 3 cut(s) 216, 736, 1104
MfeI CAATTG 1 cut(s) 1201
MhlI GDGCHC 4 cut(s) 291, 674, 1092, 1159
MlyI GAGTC 1 cut(s) 592
MmeI TCCRAC 3 cut(s) 679, 757, 1164
MnlI CCTC 9 cut(s) 173, 250, 293, 369, 380, 386, 629, 862, 1165
Mph1103I ATGCAT 1 cut(s) 963
MseI TTAA 8 cut(s) 6, 447, 539, 695, 710, 815, 953, 990
MslI CAYNNNNRTG 1 cut(s) 830
MspA1I CMGCKG 2 cut(s) 1009, 1118
MspR9I CCNGG 4 cut(s) 65, 202, 264, 907
MunI CAATTG 1 cut(s) 1201
Mva1269I GAATGC 1 cut(s) 192
MvaI CCWGG 4 cut(s) 65, 202, 264, 907
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 185
NdeII GATC 5 cut(s) 67, 228, 1179, 1183, 1196
NlaIII CATG 7 cut(s) 80, 98, 376, 799, 835, 920, 1031
NlaIV GGNNCC 3 cut(s) 261, 671, 904
NmuCI GTSAC 2 cut(s) 109, 1054
NsiI ATGCAT 1 cut(s) 963
NspV TTCGAA 1 cut(s) 123
PaeR7I CTCGAG 1 cut(s) 284
PagI TCATGA 2 cut(s) 76, 831
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 1 cut(s) 192
PfeI GAWTC 6 cut(s) 73, 608, 648, 799, 926, 1209
PflMI CCANNNNNTGG 1 cut(s) 207
PkrI GCNGC 3 cut(s) 824, 968, 1008
Ple19I CGATCG 1 cut(s) 1182
PleI GAGTC 1 cut(s) 591
PpsI GAGTC 1 cut(s) 591
Psp124BI GAGCTC 1 cut(s) 1092
Psp6I CCWGG 4 cut(s) 63, 200, 262, 905
PspGI CCWGG 4 cut(s) 63, 200, 262, 905
PspN4I GGNNCC 3 cut(s) 261, 671, 904
PspPI GGNCC 2 cut(s) 186, 482
PspXI VCTCGAGB 1 cut(s) 284
PstI CTGCAG 1 cut(s) 1102
PstNI CAGNNNCTG 1 cut(s) 1009
PvuI CGATCG 1 cut(s) 1182
PvuII CAGCTG 2 cut(s) 1009, 1118
RsaI GTAC 3 cut(s) 100, 1066, 1096
RsaNI GTAC 3 cut(s) 99, 1065, 1095
RseI CAYNNNNRTG 1 cut(s) 830
SacI GAGCTC 1 cut(s) 1092
SaqAI TTAA 8 cut(s) 6, 447, 539, 695, 710, 815, 953, 990
SatI GCNGC 3 cut(s) 823, 967, 1007
Sau3AI GATC 5 cut(s) 67, 228, 1179, 1183, 1196
Sau96I GGNCC 2 cut(s) 186, 482
ScaI AGTACT 2 cut(s) 1066, 1096
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 4 cut(s) 65, 202, 264, 907
SduI GDGCHC 4 cut(s) 291, 674, 1092, 1159
SfaNI GCATC 3 cut(s) 750, 881, 978
SfcI CTRYAG 1 cut(s) 1098
Sfr274I CTCGAG 1 cut(s) 284
SfuI TTCGAA 1 cut(s) 123
SlaI CTCGAG 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 830
SmlI CTYRAG 2 cut(s) 284, 467
SmoI CTYRAG 2 cut(s) 284, 467
SspI AATATT 2 cut(s) 809, 847
SstI GAGCTC 1 cut(s) 1092
StyD4I CCNGG 4 cut(s) 63, 200, 262, 905
TaaI ACNGT 2 cut(s) 352, 628
TaiI ACGT 2 cut(s) 830, 996
TatI WGTACW 2 cut(s) 1064, 1094
TfiI GAWTC 6 cut(s) 73, 608, 648, 799, 926, 1209
Tru1I TTAA 8 cut(s) 6, 447, 539, 695, 710, 815, 953, 990
Tru9I TTAA 8 cut(s) 6, 447, 539, 695, 710, 815, 953, 990
TscAI CASTG 2 cut(s) 274, 1159
TseFI GTSAC 2 cut(s) 109, 1054
TseI GCWGC 3 cut(s) 822, 966, 1006
Tsp45I GTSAC 2 cut(s) 109, 1054
TspDTI ATGAA 5 cut(s) 289, 640, 812, 820, 1016
TspGWI ACGGA 1 cut(s) 588
TspRI CASTG 2 cut(s) 274, 1159
Van91I CCANNNNNTGG 1 cut(s) 207
XapI RAATTY 4 cut(s) 550, 878, 1121, 1233
XcmI CCANNNNNNNNNTGG 2 cut(s) 270, 662
XhoI CTCGAG 1 cut(s) 284
ZrmI AGTACT 2 cut(s) 1066, 1096
Zsp2I ATGCAT 1 cut(s) 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.