Rh2DG582600

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000687
Physical Location & Seq
Reverse (-)
81340564 .. 81341991
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG582600.1

Sequence Viewer

Length: 1407 bp
ATGGCTAAGAAGAGAAAAATATCAGGCAGCTGCAACATGGCAAACAGAAATAAAAGGATAAAAAAGGAGGAGGAGGTTCAAAGCCAAAACACCGGGGGTATGGTGGTGGACACATACACATCCGAATTACCCGAACTTGTCATCAACCAAATCTTCTCTCATCTTTCCACCAAGTCCGCCATTAGGGCAAGCATTCTTTCTAAGGAATGGGCAAGCGTGTGTTCTTCGGTTCTCATTTTAGATATCGATGAGGATGAAGGGAAGAAGCAAATTGCTGATCACAGGGATCGACGTAGAAAGTTCCTTAAGTTCTTGAGCAATTGTTTGAAGAGCCGTGAGAAAGAAAAGTACCTGGAGAAGTTCAGGCTCCGTATGAGTTATTTTGGAGGTGACTGTGCCATAAATAAGTCATTGAGTTTTGCTTTGGAAAGAAACGTTAAAGAGTTGGATATTTGCTTCAAAAGAGAGAACGAGGTCAGAAAAGGCAGATACTATTGCTTACAGCAGACAGCTCTCAATAATGCAAAATCTTTAACCATTTTGAAGTTGGAGTATTTGAAAATAGAAGAGGACAGCATCGACACTATAAGCCTTCCGTCTTTGAAAACTTTGTCTCTAAAAGCCGTGAAATTCATGACTAACACTGGATTCTCACGCTTATATTCGGGGTGTCCTTCCATCGAGTATTTGTCATTAACTTCGTGTCACGGCTGGGATGTTAGACTTTCAAGTTCGGGGCTCAAACACTTGGAAATTCTGTGTTGCGAAATCAAACTTAAAGTCGAAGCAACAAACCTTGAATCCTTTAAATTCCGTGGTGACAATGGACTTCGTCATCATATCATTGACATGCGCAAAAACTTCTACATTGCCTCTTGTCAAACCCTCAGAAGTCTTGAGTTCTCTCATGCACACTTTGCAATTGGATGGTTCAAAGGCCTCGATTCGAGATTTCCTCTTCTTGAGAGGTTGGTTTTACATACATGCTTCAGCTACTGGGGGATTATCAACATCTTTAGCCAGCATCTGAAAAGCTTTGTGTTTCATGAATGCGGAAACATTTTGTCTAATTTTTCTATGACTGCTCCAAATTTATCCGAGGCTACAATCATAATTGAAGAGAGAAGAGACTATGATCGCCTGAGAAATTTTCTTGGAAATTTTGATTGCTCTAAGAAAATACGTCTACATGTTAATGAGACTGAGGTATGTATCTTTCCGGAGGAAATGAGAAAGAAATACTCTCCACCATTACCTAGTCTCAAGCATCTTGAGGTAGAGATAGATACTTTTACAACAGTAGTAACTCATTCGGCCTTGATGGACTCCTTACGGTGGATGGCACCGTCTCTGGAGTTTCTATCCATAAACAGAATTCATTACATGTATGATAGTAGAATTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

468

Amino Acids

54.26

Weight (kDa)

9.04

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 41 - 78 6.5e-08 F-box domain
LRR_At5g56370 PF24758 142 - 298 7.6e-10 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 854
AccB1I GGYRCC 1 cut(s) 1342
AccI GTMKAC 1 cut(s) 1186
AccIII TCCGGA 1 cut(s) 1219
AciI CCGC 2 cut(s) 177, 1053
AclI AACGTT 1 cut(s) 435
AclWI GGATC 1 cut(s) 294
AcsI RAATTY 7 cut(s) 629, 753, 809, 1090, 1147, 1159, 1374
AcuI CTGAAG 1 cut(s) 973
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 2 cut(s) 183, 1335
AflII CTTAAG 1 cut(s) 305
AflIII ACRYGT 2 cut(s) 1189, 1383
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 4 cut(s) 30, 512, 993, 1035
AluI AGCT 4 cut(s) 30, 512, 993, 1035
Alw26I GTCTC 5 cut(s) 618, 1122, 1193, 1265, 1353
AlwI GGATC 1 cut(s) 294
AlwNI CAGNNNCTG 2 cut(s) 996, 1027
Aor13HI TCCGGA 1 cut(s) 1219
AoxI GGCC 2 cut(s) 937, 1314
ApeKI GCWGC 2 cut(s) 27, 30
ApoI RAATTY 7 cut(s) 629, 753, 809, 1090, 1147, 1159, 1374
ArsI GACNNNNNNTTYG 2 cut(s) 765, 797
AspLEI GCGC 1 cut(s) 855
AsuC2I CCSGG 1 cut(s) 94
AsuHPI GGTGA 2 cut(s) 401, 830
BanI GGYRCC 1 cut(s) 1342
BanII GRGCYC 1 cut(s) 741
BbvI GCAGC 2 cut(s) 17, 39
BccI CCATC 4 cut(s) 686, 921, 1315, 1333
BceAI ACGGC 3 cut(s) 318, 608, 724
BciT130I CCWGG 1 cut(s) 353
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 94
BcoDI GTCTC 5 cut(s) 618, 1122, 1193, 1265, 1353
BfaI CTAG 1 cut(s) 1257
BfrI CTTAAG 1 cut(s) 305
BglI GCCNNNNNGGC 1 cut(s) 185
BisI GCNGC 2 cut(s) 28, 31
BlsI GCNGC 2 cut(s) 29, 32
Bme1390I CCNGG 2 cut(s) 94, 353
BmiI GGNNCC 2 cut(s) 368, 1344
BmrFI CCNGG 2 cut(s) 94, 353
BmrI ACTGGG 1 cut(s) 1006
BmsI GCATC 3 cut(s) 585, 1033, 1276
BmuI ACTGGG 1 cut(s) 1006
BplI GAGNNNNNCTC 2 cut(s) 940, 972
BpmI CTGGAG 2 cut(s) 374, 1373
BpuEI CTTGAG 5 cut(s) 334, 917, 983, 1247, 1292
BpuMI CCSGG 1 cut(s) 94
Bsa29I ATCGAT 1 cut(s) 246
BsaJI CCNNGG 3 cut(s) 93, 814, 1098
BsaWI WCCGGW 1 cut(s) 1219
Bsc4I CCNNNNNNNGG 2 cut(s) 183, 1335
Bse1I ACTGG 2 cut(s) 649, 1001
Bse3DI GCAATG 1 cut(s) 867
BseAI TCCGGA 1 cut(s) 1219
BseBI CCWGG 1 cut(s) 353
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 3 cut(s) 93, 814, 1098
BseGI GGATG 5 cut(s) 119, 259, 721, 932, 1344
BseLI CCNNNNNNNGG 2 cut(s) 183, 1335
BseMI GCAATG 1 cut(s) 867
BseMII CTCAG 3 cut(s) 901, 1133, 1194
BseNI ACTGG 2 cut(s) 649, 1001
BseRI GAGGAG 2 cut(s) 83, 86
BseXI GCAGC 2 cut(s) 17, 39
BseYI CCCAGC 1 cut(s) 711
BshFI GGCC 2 cut(s) 939, 1316
BshNI GGYRCC 1 cut(s) 1342
BshVI ATCGAT 1 cut(s) 246
BsiSI CCGG 2 cut(s) 93, 1220
BslI CCNNNNNNNGG 2 cut(s) 183, 1335
BsmAI GTCTC 5 cut(s) 618, 1122, 1193, 1265, 1353
BsmBI CGTCTC 1 cut(s) 1353
BsmI GAATGC 2 cut(s) 192, 1055
BsnI GGCC 2 cut(s) 939, 1316
Bsp1286I GDGCHC 1 cut(s) 741
Bsp13I TCCGGA 1 cut(s) 1219
Bsp143I GATC 3 cut(s) 277, 286, 1135
BspACI CCGC 2 cut(s) 177, 1053
BspANI GGCC 2 cut(s) 939, 1316
BspCNI CTCAG 3 cut(s) 900, 1134, 1195
BspDI ATCGAT 1 cut(s) 246
BspEI TCCGGA 1 cut(s) 1219
BspHI TCATGA 2 cut(s) 633, 1045
BspLI GGNNCC 2 cut(s) 368, 1344
BspPI GGATC 1 cut(s) 294
BspQI GCTCTTC 1 cut(s) 323
BspT107I GGYRCC 1 cut(s) 1342
BspTI CTTAAG 1 cut(s) 305
BsrDI GCAATG 1 cut(s) 867
BsrI ACTGG 2 cut(s) 649, 1001
BssECI CCNNGG 3 cut(s) 93, 814, 1098
BssMI GATC 3 cut(s) 277, 286, 1135
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 4 cut(s) 395, 1300, 1335, 1347
Bst6I CTCTTC 6 cut(s) 5, 323, 561, 963, 1113, 1120
BstAFI CTTAAG 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 917
BstC8I GCNNGC 3 cut(s) 190, 214, 1022
BstDEI CTNAG 6 cut(s) 6, 201, 887, 1142, 1173, 1203
BstDSI CCRYGG 1 cut(s) 814
BstF5I GGATG 5 cut(s) 119, 259, 721, 932, 1344
BstHHI GCGC 1 cut(s) 855
BstKTI GATC 3 cut(s) 280, 289, 1138
BstMAI GTCTC 5 cut(s) 618, 1122, 1193, 1265, 1353
BstMBI GATC 3 cut(s) 277, 286, 1135
BstMWI GCNNNNNNNGC 2 cut(s) 185, 917
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 4 cut(s) 853, 987, 1193, 1387
BstSCI CCNGG 2 cut(s) 92, 351
BstV1I GCAGC 2 cut(s) 17, 39
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 2 cut(s) 939, 1316
BsuTUI ATCGAT 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 814
BtsCI GGATG 5 cut(s) 119, 259, 721, 932, 1344
BtsIMutI CAGTG 1 cut(s) 642
Cac8I GCNNGC 3 cut(s) 190, 214, 1022
CaiI CAGNNNCTG 2 cut(s) 996, 1027
CciI TCATGA 2 cut(s) 633, 1045
CfoI GCGC 1 cut(s) 855
ClaI ATCGAT 1 cut(s) 246
Csp6I GTAC 1 cut(s) 349
CviAII CATG 8 cut(s) 37, 634, 850, 908, 984, 1046, 1190, 1384
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 6 cut(s) 6, 201, 887, 1142, 1173, 1203
DpnI GATC 3 cut(s) 279, 288, 1137
DpnII GATC 3 cut(s) 277, 286, 1135
DraI TTTAAA 1 cut(s) 808
Eam1104I CTCTTC 6 cut(s) 5, 323, 561, 963, 1113, 1120
EarI CTCTTC 6 cut(s) 5, 323, 561, 963, 1113, 1120
EciI GGCGGA 1 cut(s) 166
Eco147I AGGCCT 1 cut(s) 939
Eco24I GRGCYC 1 cut(s) 741
Eco32I GATATC 1 cut(s) 244
Eco57I CTGAAG 1 cut(s) 973
EcoRI GAATTC 1 cut(s) 1374
EcoRII CCWGG 1 cut(s) 351
EcoRV GATATC 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 741
Esp3I CGTCTC 1 cut(s) 1353
FaeI CATG 8 cut(s) 40, 637, 853, 911, 987, 1049, 1193, 1387
FatI CATG 8 cut(s) 36, 633, 849, 907, 983, 1045, 1189, 1383
FbaI TGATCA 1 cut(s) 277
FblI GTMKAC 1 cut(s) 1186
Fnu4HI GCNGC 2 cut(s) 28, 31
FokI GGATG 5 cut(s) 106, 266, 728, 939, 1351
FriOI GRGCYC 1 cut(s) 741
Fsp4HI GCNGC 2 cut(s) 28, 31
FspBI CTAG 1 cut(s) 1257
FspI TGCGCA 1 cut(s) 854
GlaI GCGC 1 cut(s) 854
GluI GCNGC 2 cut(s) 28, 31
GsaI CCCAGC 1 cut(s) 715
GsuI CTGGAG 2 cut(s) 374, 1373
HaeIII GGCC 2 cut(s) 939, 1316
HapII CCGG 2 cut(s) 93, 1220
HhaI GCGC 1 cut(s) 855
Hin1II CATG 8 cut(s) 40, 637, 853, 911, 987, 1049, 1193, 1387
Hin6I GCGC 1 cut(s) 853
HinP1I GCGC 1 cut(s) 853
HindIII AAGCTT 1 cut(s) 1033
HinfI GANTC 4 cut(s) 648, 800, 944, 1325
HpaII CCGG 2 cut(s) 93, 1220
HphI GGTGA 2 cut(s) 401, 830
Hpy166II GTNNAC 2 cut(s) 109, 1187
Hpy188I TCNGA 5 cut(s) 124, 479, 890, 1029, 1099
Hpy188III TCNNGA 9 cut(s) 313, 634, 896, 948, 962, 1046, 1220, 1271, 1352
Hpy8I GTNNAC 2 cut(s) 109, 1187
Hpy99I CGWCG 1 cut(s) 294
HpyAV CCTTC 3 cut(s) 251, 602, 684
HpyCH4III ACNGT 4 cut(s) 395, 1300, 1335, 1347
HpyCH4IV ACGT 3 cut(s) 292, 435, 1183
HpyCH4V TGCA 4 cut(s) 33, 524, 911, 920
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 917
HpyF3I CTNAG 6 cut(s) 6, 201, 887, 1142, 1173, 1203
HpySE526I ACGT 3 cut(s) 292, 435, 1183
Hsp92II CATG 8 cut(s) 40, 637, 853, 911, 987, 1049, 1193, 1387
HspAI GCGC 1 cut(s) 853
Kpn2I TCCGGA 1 cut(s) 1219
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 3 cut(s) 277, 286, 1135
LguI GCTCTTC 1 cut(s) 323
LmnI GCTCC 2 cut(s) 372, 1090
Lsp1109I GCAGC 2 cut(s) 17, 39
LweI GCATC 3 cut(s) 585, 1033, 1276
MaeI CTAG 1 cut(s) 1257
MaeII ACGT 3 cut(s) 292, 435, 1183
MaeIII GTNAC 4 cut(s) 389, 704, 818, 1303
MalI GATC 3 cut(s) 279, 288, 1137
MboI GATC 3 cut(s) 277, 286, 1135
MboII GAAGA 9 cut(s) 22, 145, 216, 274, 340, 578, 950, 1130, 1137
MfeI CAATTG 2 cut(s) 319, 921
MhlI GDGCHC 1 cut(s) 741
MlyI GAGTC 1 cut(s) 1319
MmeI TCCRAC 2 cut(s) 426, 528
MroI TCCGGA 1 cut(s) 1219
MseI TTAA 8 cut(s) 306, 438, 533, 695, 777, 807, 1194, 1405
MslI CAYNNNNRTG 2 cut(s) 848, 1194
MspA1I CMGCKG 1 cut(s) 30
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 2 cut(s) 93, 1220
MspR9I CCNGG 2 cut(s) 94, 353
MunI CAATTG 2 cut(s) 319, 921
Mva1269I GAATGC 2 cut(s) 192, 1055
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 2 cut(s) 185, 917
NciI CCSGG 1 cut(s) 94
NdeII GATC 3 cut(s) 277, 286, 1135
NlaIII CATG 8 cut(s) 40, 637, 853, 911, 987, 1049, 1193, 1387
NlaIV GGNNCC 2 cut(s) 368, 1344
NmuCI GTSAC 3 cut(s) 389, 704, 818
NsbI TGCGCA 1 cut(s) 854
NspI RCATGY 4 cut(s) 853, 987, 1193, 1387
PagI TCATGA 2 cut(s) 633, 1045
PceI AGGCCT 1 cut(s) 939
PciI ACATGT 2 cut(s) 1189, 1383
PciSI GCTCTTC 1 cut(s) 323
PctI GAATGC 2 cut(s) 192, 1055
PfeI GAWTC 3 cut(s) 648, 800, 944
PflFI GACNNNGTC 1 cut(s) 831
PkrI GCNGC 2 cut(s) 29, 32
PleI GAGTC 1 cut(s) 1319
PpsI GAGTC 1 cut(s) 1319
PscI ACATGT 2 cut(s) 1189, 1383
Psp1406I AACGTT 1 cut(s) 435
Psp6I CCWGG 1 cut(s) 351
PspFI CCCAGC 1 cut(s) 711
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 2 cut(s) 368, 1344
PstNI CAGNNNCTG 2 cut(s) 996, 1027
PsyI GACNNNGTC 1 cut(s) 831
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
RseI CAYNNNNRTG 2 cut(s) 848, 1194
SapI GCTCTTC 1 cut(s) 323
SaqAI TTAA 8 cut(s) 306, 438, 533, 695, 777, 807, 1194, 1405
SatI GCNGC 2 cut(s) 28, 31
Sau3AI GATC 3 cut(s) 277, 286, 1135
SchI GAGTC 1 cut(s) 1319
ScrFI CCNGG 2 cut(s) 94, 353
SduI GDGCHC 1 cut(s) 741
SfaNI GCATC 3 cut(s) 585, 1033, 1276
SmiMI CAYNNNNRTG 2 cut(s) 848, 1194
SmlI CTYRAG 6 cut(s) 305, 313, 896, 962, 1262, 1271
SmoI CTYRAG 6 cut(s) 305, 313, 896, 962, 1262, 1271
SseBI AGGCCT 1 cut(s) 939
SsiI CCGC 2 cut(s) 177, 1053
SspMI CTAG 1 cut(s) 1257
StuI AGGCCT 1 cut(s) 939
StyD4I CCNGG 2 cut(s) 92, 351
TaaI ACNGT 4 cut(s) 395, 1300, 1335, 1347
TaiI ACGT 3 cut(s) 295, 438, 1186
TaqI TCGA 7 cut(s) 246, 289, 579, 681, 783, 942, 947
TfiI GAWTC 3 cut(s) 648, 800, 944
Tru1I TTAA 8 cut(s) 306, 438, 533, 695, 777, 807, 1194, 1405
Tru9I TTAA 8 cut(s) 306, 438, 533, 695, 777, 807, 1194, 1405
TscAI CASTG 1 cut(s) 649
TseFI GTSAC 3 cut(s) 389, 704, 818
TseI GCWGC 2 cut(s) 27, 30
Tsp45I GTSAC 3 cut(s) 389, 704, 818
TspDTI ATGAA 5 cut(s) 270, 622, 1034, 1062, 1367
TspGWI ACGGA 3 cut(s) 359, 585, 803
TspRI CASTG 1 cut(s) 649
Tth111I GACNNNGTC 1 cut(s) 831
Vha464I CTTAAG 1 cut(s) 305
XapI RAATTY 7 cut(s) 629, 753, 809, 1090, 1147, 1159, 1374
XceI RCATGY 4 cut(s) 853, 987, 1193, 1387
XcmI CCANNNNNNNNNTGG 1 cut(s) 544
XmiI GTMKAC 1 cut(s) 1186
XspI CTAG 1 cut(s) 1257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.