FvH4_6g29733

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000619
Physical Location & Seq
Forward (+)
22966877 .. 22967233
357 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29733.t1

Sequence Viewer

Length: 357 bp
ATGAATGATGTTCGAAGAAAGATTGGGTGGAGAAACGCGTTTACAGTCAATTGTAAAGTTGTGCGACAAAAGCATGGAAAGCGAGTCAGCCGAGCAGGAGGTCTCTCCATTTTGTGGAATGATGATATCAAGTTGGAGTTGCAGTCTTATAGTGAGAGTCATATTGATGTGCTAGTCAGAGAAGATGAGGGTAATCAAGTGTGGAGATTCACTGGAGTGTACGGCCAGCCTAGGGTGGAGAACAGATACTTAACGTGGGAGCTTCTCAAGAAACTTGGGAGTCAAGACAAAGGTCCTTGGTTGTTAGGAGGAGATTTTAATGAGATTCTTTGGGCTATGGAGAAGGAGGGAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

119

Amino Acids

13.72

Weight (kDa)

9.13

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 26 - 114 4.5e-06 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000619)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29861 FvH4_3g26084 FvH4_3g32551 FvH4_4g08193 FvH4_4g12950 FvH4_6g29733
rosa_chinensis RchiOBHm_Chr3g0488921
rosa_multiflora Rmu_co8238041.1_g000001 Rmu_co8265215.1_g000001 Rmu_co8486357.1_g000002 Rmu_sc0000389.1_g000016 Rmu_sc0000580.1_g000123 Rmu_sc0000789.1_g000005 Rmu_sc0001043.1_g000006 Rmu_sc0001208.1_g000001 Rmu_sc0001323.1_g000011 Rmu_sc0001636.1_g000001 Rmu_sc0001911.1_g000023 Rmu_sc0001940.1_g000010 Rmu_sc0002531.1_g000047 Rmu_sc0002715.1_g000007 Rmu_sc0002833.1_g000024 Rmu_sc0003859.1_g000046 Rmu_sc0003894.1_g000008 Rmu_sc0004744.1_g000008 Rmu_sc0004924.1_g000016 Rmu_sc0004991.1_g000004 Rmu_sc0005149.1_g000010 Rmu_sc0005149.1_g000011 Rmu_sc0005489.1_g000007 Rmu_sc0005592.1_g000030 Rmu_sc0005813.1_g000010 Rmu_sc0005813.1_g000011 Rmu_sc0005861.1_g000009 Rmu_sc0005949.1_g000016 Rmu_sc0006824.1_g000021 Rmu_sc0007069.1_g000025 Rmu_sc0008002.1_g000008 Rmu_sc0008148.1_g000008 Rmu_sc0008280.1_g000001 Rmu_sc0008317.1_g000010 Rmu_sc0011615.1_g000002 Rmu_sc0013925.1_g000004 Rmu_sc0014130.1_g000003 Rmu_sc0014510.1_g000008 Rmu_sc0016640.1_g000001 Rmu_sc0018068.1_g000002 Rmu_sc0021371.1_g000006 Rmu_sc0028652.1_g000007 Rmu_sc0032187.1_g000002 Rmu_sc0034241.1_g000001 Rmu_ssc0000127.1_g000003
rosa_roxburghii Rroxscaffold_6G00403950
rosa_rugosa Rorug01G0081700 Rorug02G0544000 Rorug05G0089900 Rorug05G0284500 Rorug05G0452400 Rorug07G0003400 Rorug07G0003400 Rorug07G0129200 Rorug07G0210600
rosa_samantha Rh1AG193900 Rh1AG194000 Rh1DG063400 Rh1DG204700 Rh2AG364300 Rh3BG140200 Rh3CG366000 Rh4DG342600 Rh5AG347700 Rh5BG437300 Rh5CG459900 Rh5DG127400 Rh5DG412800 Rh6BG381500 Rh7CG520500 Rh7DG241900 Rh7DG336000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 114
AccII CGCG 1 cut(s) 38
AcoI YGGCCR 1 cut(s) 223
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 2 cut(s) 114, 232
AflIII ACRYGT 1 cut(s) 36
AleI CACNNNNGTG 1 cut(s) 215
AluBI AGCT 1 cut(s) 262
AluI AGCT 1 cut(s) 262
Alw26I GTCTC 1 cut(s) 107
AoxI GGCC 1 cut(s) 223
AspA2I CCTAGG 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 293
AsuII TTCGAA 1 cut(s) 13
AvaII GGWCC 1 cut(s) 293
AvrII CCTAGG 1 cut(s) 230
BceAI ACGGC 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 107
BfaI CTAG 2 cut(s) 173, 231
BlnI CCTAGG 1 cut(s) 230
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 293
BoxI GACNNNNGTC 1 cut(s) 291
BpmI CTGGAG 1 cut(s) 234
Bpu14I TTCGAA 1 cut(s) 13
BpuEI CTTGAG 1 cut(s) 251
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 2 cut(s) 230, 296
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 232
Bse1I ACTGG 1 cut(s) 217
BseDI CCNNGG 2 cut(s) 230, 296
BseLI CCNNNNNNNGG 2 cut(s) 114, 232
BseNI ACTGG 1 cut(s) 217
BseRI GAGGAG 1 cut(s) 324
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 1 cut(s) 225
BslI CCNNNNNNNGG 2 cut(s) 114, 232
BsmAI GTCTC 1 cut(s) 107
BsnI GGCC 1 cut(s) 225
Bso31I GGTCTC 1 cut(s) 107
Bsp119I TTCGAA 1 cut(s) 13
BspANI GGCC 1 cut(s) 225
BspFNI CGCG 1 cut(s) 38
BspT104I TTCGAA 1 cut(s) 13
BspTNI GGTCTC 1 cut(s) 107
BsrI ACTGG 1 cut(s) 217
BssECI CCNNGG 2 cut(s) 230, 296
BssT1I CCWWGG 2 cut(s) 230, 296
Bst4CI ACNGT 1 cut(s) 46
BstBI TTCGAA 1 cut(s) 13
BstC8I GCNNGC 1 cut(s) 227
BstFNI CGCG 1 cut(s) 38
BstMAI GTCTC 1 cut(s) 107
BstMWI GCNNNNNNNGC 2 cut(s) 70, 79
BstPAI GACNNNNGTC 1 cut(s) 291
BstUI CGCG 1 cut(s) 38
BsuRI GGCC 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 210
Cac8I GCNNGC 1 cut(s) 227
Cfr13I GGNCC 1 cut(s) 293
Csp6I GTAC 1 cut(s) 220
CviAII CATG 1 cut(s) 74
CviJI RGCY 6 cut(s) 90, 225, 229, 262, 335, 354
CviKI_1 RGCY 6 cut(s) 90, 225, 229, 262, 335, 354
CviQI GTAC 1 cut(s) 220
EaeI YGGCCR 1 cut(s) 223
Eco130I CCWWGG 2 cut(s) 230, 296
Eco31I GGTCTC 1 cut(s) 107
Eco32I GATATC 1 cut(s) 127
Eco47I GGWCC 1 cut(s) 293
EcoO109I RGGNCCY 1 cut(s) 293
EcoRV GATATC 1 cut(s) 127
EcoT14I CCWWGG 2 cut(s) 230, 296
ErhI CCWWGG 2 cut(s) 230, 296
FaeI CATG 1 cut(s) 77
FaiI YATR 4 cut(s) 75, 150, 162, 338
FatI CATG 1 cut(s) 73
FspBI CTAG 2 cut(s) 173, 231
GsuI CTGGAG 1 cut(s) 234
HaeIII GGCC 1 cut(s) 225
Hin1II CATG 1 cut(s) 77
HinfI GANTC 5 cut(s) 84, 157, 207, 280, 325
Hpy166II GTNNAC 2 cut(s) 42, 220
Hpy188I TCNGA 1 cut(s) 179
Hpy188III TCNNGA 2 cut(s) 268, 284
Hpy8I GTNNAC 2 cut(s) 42, 220
HpyAV CCTTC 1 cut(s) 337
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4IV ACGT 1 cut(s) 254
HpyCH4V TGCA 1 cut(s) 142
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 79
HpySE526I ACGT 1 cut(s) 254
Hsp92II CATG 1 cut(s) 77
LmnI GCTCC 1 cut(s) 259
LpnPI CCDG 3 cut(s) 81, 198, 239
MaeI CTAG 2 cut(s) 173, 231
MaeII ACGT 1 cut(s) 254
MboII GAAGA 2 cut(s) 27, 194
MfeI CAATTG 1 cut(s) 49
MluCI AATT 1 cut(s) 49
MluI ACGCGT 1 cut(s) 36
MlyI GAGTC 3 cut(s) 93, 166, 289
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 5 cut(s) 92, 181, 302, 340, 344
MseI TTAA 2 cut(s) 251, 318
MslI CAYNNNNRTG 2 cut(s) 165, 215
MunI CAATTG 1 cut(s) 49
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 2 cut(s) 70, 79
NlaIII CATG 1 cut(s) 77
NmeAIII GCCGAG 1 cut(s) 116
NspV TTCGAA 1 cut(s) 13
OliI CACNNNNGTG 1 cut(s) 215
PfeI GAWTC 2 cut(s) 207, 325
PflMI CCANNNNNTGG 1 cut(s) 114
PleI GAGTC 3 cut(s) 92, 165, 288
PpsI GAGTC 3 cut(s) 92, 165, 288
PpuMI RGGWCCY 1 cut(s) 293
PshAI GACNNNNGTC 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 293
PspPI GGNCC 1 cut(s) 293
PspPPI RGGWCCY 1 cut(s) 293
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
RseI CAYNNNNRTG 2 cut(s) 165, 215
SaqAI TTAA 2 cut(s) 251, 318
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 3 cut(s) 93, 166, 289
SetI ASST 4 cut(s) 103, 257, 264, 295
SfuI TTCGAA 1 cut(s) 13
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 2 cut(s) 165, 215
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 1 cut(s) 49
SspMI CTAG 2 cut(s) 173, 231
StyI CCWWGG 2 cut(s) 230, 296
TaaI ACNGT 1 cut(s) 46
TaiI ACGT 1 cut(s) 257
TaqI TCGA 1 cut(s) 13
TasI AATT 1 cut(s) 49
TfiI GAWTC 2 cut(s) 207, 325
Tru1I TTAA 2 cut(s) 251, 318
Tru9I TTAA 2 cut(s) 251, 318
TscAI CASTG 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 217
Van91I CCANNNNNTGG 1 cut(s) 114
VpaK11BI GGWCC 1 cut(s) 293
XmaJI CCTAGG 1 cut(s) 230
XspI CTAG 2 cut(s) 173, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.