Rmu_sc0000789.1_g000005

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000789.1
OG0000619
Physical Location & Seq
Reverse (-)
31644 .. 32484
841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000789.1_g000005.1.cds

Sequence Viewer

Length: 756 bp
atgttcgggatcagttggggtggagctagaaatgcgatttcggtgtctctccaatctctttcggagagtcatattgatgtggttgtgagcactgttggtgaacccgagtgttggcgctttacaggtttatacggccaacctagggctgagaacagacaacagacttggaacttattgcgccagtttagtctgcaggggaatcttccatgggtggttggtggtgattataatgagatttgtgtggcgtctgacaagttgggtggcgctctaagatgtgccacacagatgaataacatgcatgaggctttggctttttgtgagttaggagatatacaatatgtaggccccagatttacgtggagtggcataagagggggtgaggaggtgaaagtccggcttgacagatttacttcaacgcttcaatggcgagacttatttccggcttctcgtgtcaggcaccctgatccgagtgcttccgaccatctaccaatccttctggagatcagggttcagaaaaaagggagaaaaaaaaggaagaagaggtttaaattcgaagagttttggttattagaggataagtgcaaggagattgttagagattgttggggaagtttgttcagtggtagatcgtttgcgtccttgtgtcataagcttaataggactcgtgatgccttggagtcttggagtagggagaattttggtggcttaaaggagagatatcgagagagtcttgtcccaacttgctgccttctatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

251

Amino Acids

28.8

Weight (kDa)

9.11

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000619)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29861 FvH4_3g26084 FvH4_3g32551 FvH4_4g08193 FvH4_4g12950 FvH4_6g29733
rosa_chinensis RchiOBHm_Chr3g0488921
rosa_multiflora Rmu_co8238041.1_g000001 Rmu_co8265215.1_g000001 Rmu_co8486357.1_g000002 Rmu_sc0000389.1_g000016 Rmu_sc0000580.1_g000123 Rmu_sc0000789.1_g000005 Rmu_sc0001043.1_g000006 Rmu_sc0001208.1_g000001 Rmu_sc0001323.1_g000011 Rmu_sc0001636.1_g000001 Rmu_sc0001911.1_g000023 Rmu_sc0001940.1_g000010 Rmu_sc0002531.1_g000047 Rmu_sc0002715.1_g000007 Rmu_sc0002833.1_g000024 Rmu_sc0003859.1_g000046 Rmu_sc0003894.1_g000008 Rmu_sc0004744.1_g000008 Rmu_sc0004924.1_g000016 Rmu_sc0004991.1_g000004 Rmu_sc0005149.1_g000010 Rmu_sc0005149.1_g000011 Rmu_sc0005489.1_g000007 Rmu_sc0005592.1_g000030 Rmu_sc0005813.1_g000010 Rmu_sc0005813.1_g000011 Rmu_sc0005861.1_g000009 Rmu_sc0005949.1_g000016 Rmu_sc0006824.1_g000021 Rmu_sc0007069.1_g000025 Rmu_sc0008002.1_g000008 Rmu_sc0008148.1_g000008 Rmu_sc0008280.1_g000001 Rmu_sc0008317.1_g000010 Rmu_sc0011615.1_g000002 Rmu_sc0013925.1_g000004 Rmu_sc0014130.1_g000003 Rmu_sc0014510.1_g000008 Rmu_sc0016640.1_g000001 Rmu_sc0018068.1_g000002 Rmu_sc0021371.1_g000006 Rmu_sc0028652.1_g000007 Rmu_sc0032187.1_g000002 Rmu_sc0034241.1_g000001 Rmu_ssc0000127.1_g000003
rosa_roxburghii Rroxscaffold_6G00403950
rosa_rugosa Rorug01G0081700 Rorug02G0544000 Rorug05G0089900 Rorug05G0284500 Rorug05G0452400 Rorug07G0003400 Rorug07G0003400 Rorug07G0129200 Rorug07G0210600
rosa_samantha Rh1AG193900 Rh1AG194000 Rh1DG063400 Rh1DG204700 Rh2AG364300 Rh3BG140200 Rh3CG366000 Rh4DG342600 Rh5AG347700 Rh5BG437300 Rh5CG459900 Rh5DG127400 Rh5DG412800 Rh6BG381500 Rh7CG520500 Rh7DG241900 Rh7DG336000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 228
AccB1I GGYRCC 1 cut(s) 456
AclWI GGATC 2 cut(s) 17, 458
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 2 cut(s) 548, 694
AcyI GRCGYC 1 cut(s) 245
AfiI CCNNNNNNNGG 2 cut(s) 111, 142
AgsI TTSAA 2 cut(s) 414, 422
AluBI AGCT 2 cut(s) 26, 652
AluI AGCT 2 cut(s) 26, 652
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 2 cut(s) 51, 423
AlwI GGATC 2 cut(s) 17, 458
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 2 cut(s) 133, 343
ApeKI GCWGC 1 cut(s) 744
ApoI RAATTY 2 cut(s) 548, 694
AspA2I CCTAGG 1 cut(s) 140
AspLEI GCGC 3 cut(s) 117, 180, 266
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 4 cut(s) 110, 233, 389, 397
AsuII TTCGAA 1 cut(s) 552
AvaI CYCGRG 1 cut(s) 104
AvrII CCTAGG 1 cut(s) 140
BanI GGYRCC 1 cut(s) 456
BauI CACGAG 2 cut(s) 447, 663
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 1 cut(s) 731
BccI CCATC 1 cut(s) 489
BceAI ACGGC 1 cut(s) 148
BcoDI GTCTC 2 cut(s) 51, 423
BfaI CTAG 2 cut(s) 27, 141
BfmI CTRYAG 1 cut(s) 191
BfoI RGCGCY 2 cut(s) 118, 267
BisI GCNGC 1 cut(s) 745
BlnI CCTAGG 1 cut(s) 140
BlsI GCNGC 1 cut(s) 746
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 2 cut(s) 346, 458
BmsI GCATC 1 cut(s) 658
BpmI CTGGAG 1 cut(s) 518
Bpu14I TTCGAA 1 cut(s) 552
BsaAI YACGTR 1 cut(s) 357
BsaHI GRCGYC 1 cut(s) 245
BsaJI CCNNGG 3 cut(s) 140, 206, 672
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 142
Bse1I ACTGG 1 cut(s) 181
BseDI CCNNGG 3 cut(s) 140, 206, 672
BseLI CCNNNNNNNGG 2 cut(s) 111, 142
BseMII CTCAG 1 cut(s) 138
BseNI ACTGG 1 cut(s) 181
BseRI GAGGAG 1 cut(s) 395
BseXI GCAGC 1 cut(s) 731
BshFI GGCC 2 cut(s) 135, 345
BshNI GGYRCC 1 cut(s) 456
BsiHKAI GWGCWC 1 cut(s) 92
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 2 cut(s) 394, 440
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 2 cut(s) 111, 142
BsmAI GTCTC 2 cut(s) 51, 423
BsmFI GGGAC 1 cut(s) 719
BsnI GGCC 2 cut(s) 135, 345
BsoBI CYCGRG 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 552
Bsp1286I GDGCHC 1 cut(s) 92
Bsp143I GATC 4 cut(s) 9, 463, 501, 626
Bsp19I CCATGG 1 cut(s) 206
BspANI GGCC 2 cut(s) 135, 345
BspCNI CTCAG 1 cut(s) 139
BspLI GGNNCC 2 cut(s) 346, 458
BspMAI CTGCAG 1 cut(s) 195
BspPI GGATC 2 cut(s) 17, 458
BspT104I TTCGAA 1 cut(s) 552
BspT107I GGYRCC 1 cut(s) 456
BsrI ACTGG 1 cut(s) 181
BssECI CCNNGG 3 cut(s) 140, 206, 672
BssMI GATC 4 cut(s) 9, 463, 501, 626
BssNI GRCGYC 1 cut(s) 245
BssSI CACGAG 2 cut(s) 447, 663
BssT1I CCWWGG 3 cut(s) 140, 206, 672
Bst2BI CACGAG 2 cut(s) 447, 663
Bst4CI ACNGT 1 cut(s) 94
Bst6I CTCTTC 2 cut(s) 533, 549
BstACI GRCGYC 1 cut(s) 245
BstBAI YACGTR 1 cut(s) 357
BstBI TTCGAA 1 cut(s) 552
BstDEI CTNAG 2 cut(s) 147, 269
BstDSI CCRYGG 1 cut(s) 206
BstH2I RGCGCY 2 cut(s) 118, 267
BstHHI GCGC 3 cut(s) 117, 180, 266
BstKTI GATC 4 cut(s) 12, 466, 504, 629
BstMAI GTCTC 2 cut(s) 51, 423
BstMBI GATC 4 cut(s) 9, 463, 501, 626
BstMWI GCNNNNNNNGC 2 cut(s) 32, 424
BstNSI RCATGY 1 cut(s) 298
BstSFI CTRYAG 1 cut(s) 191
BstV1I GCAGC 1 cut(s) 731
BsuRI GGCC 2 cut(s) 135, 345
BtgI CCRYGG 1 cut(s) 206
BtsIMutI CAGTG 2 cut(s) 90, 625
CfoI GCGC 3 cut(s) 117, 180, 266
Cfr13I GGNCC 1 cut(s) 344
CseI GACGC 2 cut(s) 234, 624
CspCI CAANNNNNGTGG 2 cut(s) 241, 276
CviAII CATG 3 cut(s) 207, 295, 299
DdeI CTNAG 2 cut(s) 147, 269
DpnI GATC 4 cut(s) 11, 465, 503, 628
DpnII GATC 4 cut(s) 9, 463, 501, 626
DraI TTTAAA 1 cut(s) 547
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 2 cut(s) 533, 549
EarI CTCTTC 2 cut(s) 533, 549
Eco130I CCWWGG 3 cut(s) 140, 206, 672
Eco32I GATATC 1 cut(s) 719
Eco88I CYCGRG 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 344
EcoRV GATATC 1 cut(s) 719
EcoT14I CCWWGG 3 cut(s) 140, 206, 672
EcoT22I ATGCAT 1 cut(s) 300
ErhI CCWWGG 3 cut(s) 140, 206, 672
FaeI CATG 3 cut(s) 210, 298, 302
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 1 cut(s) 719
FatI CATG 3 cut(s) 206, 294, 298
Fnu4HI GCNGC 1 cut(s) 745
Fsp4HI GCNGC 1 cut(s) 745
FspBI CTAG 2 cut(s) 27, 141
GlaI GCGC 3 cut(s) 116, 179, 265
GluI GCNGC 1 cut(s) 745
GsuI CTGGAG 1 cut(s) 518
HaeII RGCGCY 2 cut(s) 118, 267
HaeIII GGCC 2 cut(s) 135, 345
HapII CCGG 2 cut(s) 394, 440
HgaI GACGC 2 cut(s) 234, 624
HhaI GCGC 3 cut(s) 117, 180, 266
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 3 cut(s) 210, 298, 302
Hin6I GCGC 3 cut(s) 115, 178, 264
HinP1I GCGC 3 cut(s) 115, 178, 264
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 5 cut(s) 67, 199, 661, 677, 727
HpaII CCGG 2 cut(s) 394, 440
HphI GGTGA 4 cut(s) 110, 233, 389, 397
Hpy166II GTNNAC 1 cut(s) 101
Hpy188I TCNGA 5 cut(s) 64, 250, 468, 478, 513
Hpy188III TCNNGA 4 cut(s) 7, 497, 665, 722
Hpy8I GTNNAC 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 503
HpyCH4III ACNGT 1 cut(s) 94
HpyCH4IV ACGT 1 cut(s) 356
HpyCH4V TGCA 3 cut(s) 193, 298, 582
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 424
HpyF3I CTNAG 2 cut(s) 147, 269
HpySE526I ACGT 1 cut(s) 356
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 3 cut(s) 210, 298, 302
HspAI GCGC 3 cut(s) 115, 178, 264
Kzo9I GATC 4 cut(s) 9, 463, 501, 626
LmnI GCTCC 1 cut(s) 23
Lsp1109I GCAGC 1 cut(s) 731
LweI GCATC 1 cut(s) 658
MaeI CTAG 2 cut(s) 27, 141
MaeII ACGT 1 cut(s) 356
MalI GATC 4 cut(s) 11, 465, 503, 628
MboI GATC 4 cut(s) 9, 463, 501, 626
MboII GAAGA 4 cut(s) 194, 547, 550, 566
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 2 cut(s) 548, 694
MlyI GAGTC 4 cut(s) 76, 655, 686, 736
MmeI TCCRAC 1 cut(s) 501
MnlI CCTC 6 cut(s) 295, 365, 373, 376, 534, 565
Mph1103I ATGCAT 1 cut(s) 300
MseI TTAA 3 cut(s) 546, 654, 707
MslI CAYNNNNRTG 2 cut(s) 75, 284
MspI CCGG 2 cut(s) 394, 440
MwoI GCNNNNNNNGC 2 cut(s) 32, 424
NcoI CCATGG 1 cut(s) 206
NdeII GATC 4 cut(s) 9, 463, 501, 626
NlaIII CATG 3 cut(s) 210, 298, 302
NlaIV GGNNCC 2 cut(s) 346, 458
NsiI ATGCAT 1 cut(s) 300
NspI RCATGY 1 cut(s) 298
NspV TTCGAA 1 cut(s) 552
PfeI GAWTC 1 cut(s) 199
PkrI GCNGC 1 cut(s) 746
PleI GAGTC 4 cut(s) 75, 655, 685, 735
PpsI GAGTC 4 cut(s) 75, 655, 685, 735
Ppu21I YACGTR 1 cut(s) 357
PsiI TTATAA 1 cut(s) 228
PspN4I GGNNCC 2 cut(s) 346, 458
PspPI GGNCC 1 cut(s) 344
PstI CTGCAG 1 cut(s) 195
RseI CAYNNNNRTG 2 cut(s) 75, 284
SaqAI TTAA 3 cut(s) 546, 654, 707
SatI GCNGC 1 cut(s) 745
Sau3AI GATC 4 cut(s) 9, 463, 501, 626
Sau96I GGNCC 1 cut(s) 344
SchI GAGTC 4 cut(s) 76, 655, 686, 736
SduI GDGCHC 1 cut(s) 92
SetI ASST 7 cut(s) 28, 127, 142, 359, 387, 545, 654
SfaNI GCATC 1 cut(s) 658
SfcI CTRYAG 1 cut(s) 191
SfuI TTCGAA 1 cut(s) 552
SmiMI CAYNNNNRTG 2 cut(s) 75, 284
Sse9I AATT 2 cut(s) 548, 694
SspMI CTAG 2 cut(s) 27, 141
StyI CCWWGG 3 cut(s) 140, 206, 672
TaaI ACNGT 1 cut(s) 94
TaiI ACGT 1 cut(s) 359
TaqI TCGA 2 cut(s) 552, 721
TasI AATT 2 cut(s) 548, 694
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 3 cut(s) 546, 654, 707
Tru9I TTAA 3 cut(s) 546, 654, 707
TscAI CASTG 2 cut(s) 97, 625
TseI GCWGC 1 cut(s) 744
TspDTI ATGAA 1 cut(s) 302
TspRI CASTG 2 cut(s) 97, 625
XapI RAATTY 2 cut(s) 548, 694
XceI RCATGY 1 cut(s) 298
XmaJI CCTAGG 1 cut(s) 140
XspI CTAG 2 cut(s) 27, 141
Zsp2I ATGCAT 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.