Rmu_sc0005592.1_g000030

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005592.1
OG0000619
Physical Location & Seq
Reverse (-)
101003 .. 101806
804 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005592.1_g000030.1.cds

Sequence Viewer

Length: 690 bp
atgtctaatattcgtgtgcagcttgggtggcctaacgtatttgcagttccttgtatctttaaacaaaagaagaatggtaagggtgttagtcgtagtggtggtttgtgtttactttggtcgggggatgtggtggtttcgttgcgatcctattcaaataatcacattgatgtcttagtgggtaagccgaatgatccgaatagatggcgattcacaggtgtgtatggtttcccaaaagttgaagacatgcatcgaacttggagtctccttaagactcttggacaccaaattcagcttccgtgtgtcttaggtggagattttaatgagattattgcgaacaacgaaaaagaaggaggtttgttgcgaagccaacttcaaatggatggctttagggatttcatgaattctttcgctttacgccacataccggcagttaatttgaaccctaataggagtgaccatcttccaattctgttagacatcaagcaacgcagacaacaaaagaaaaaacggaagaagaagaggttccgcttcgaggaaatgtggcttagagacaaggagtgtttaaaggtggtagaagatgggtggaacagtgggcatggtgacaatcctttctctagtgtttgtagtaagttagactgcactagacgtgctctgatggagtggagtgaggaaagatttggaggcctttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

229

Amino Acids

26.4

Weight (kDa)

9.72

Isoelectric Point (pI)

61.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000619)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29861 FvH4_3g26084 FvH4_3g32551 FvH4_4g08193 FvH4_4g12950 FvH4_6g29733
rosa_chinensis RchiOBHm_Chr3g0488921
rosa_multiflora Rmu_co8238041.1_g000001 Rmu_co8265215.1_g000001 Rmu_co8486357.1_g000002 Rmu_sc0000389.1_g000016 Rmu_sc0000580.1_g000123 Rmu_sc0000789.1_g000005 Rmu_sc0001043.1_g000006 Rmu_sc0001208.1_g000001 Rmu_sc0001323.1_g000011 Rmu_sc0001636.1_g000001 Rmu_sc0001911.1_g000023 Rmu_sc0001940.1_g000010 Rmu_sc0002531.1_g000047 Rmu_sc0002715.1_g000007 Rmu_sc0002833.1_g000024 Rmu_sc0003859.1_g000046 Rmu_sc0003894.1_g000008 Rmu_sc0004744.1_g000008 Rmu_sc0004924.1_g000016 Rmu_sc0004991.1_g000004 Rmu_sc0005149.1_g000010 Rmu_sc0005149.1_g000011 Rmu_sc0005489.1_g000007 Rmu_sc0005592.1_g000030 Rmu_sc0005813.1_g000010 Rmu_sc0005813.1_g000011 Rmu_sc0005861.1_g000009 Rmu_sc0005949.1_g000016 Rmu_sc0006824.1_g000021 Rmu_sc0007069.1_g000025 Rmu_sc0008002.1_g000008 Rmu_sc0008148.1_g000008 Rmu_sc0008280.1_g000001 Rmu_sc0008317.1_g000010 Rmu_sc0011615.1_g000002 Rmu_sc0013925.1_g000004 Rmu_sc0014130.1_g000003 Rmu_sc0014510.1_g000008 Rmu_sc0016640.1_g000001 Rmu_sc0018068.1_g000002 Rmu_sc0021371.1_g000006 Rmu_sc0028652.1_g000007 Rmu_sc0032187.1_g000002 Rmu_sc0034241.1_g000001 Rmu_ssc0000127.1_g000003
rosa_roxburghii Rroxscaffold_6G00403950
rosa_rugosa Rorug01G0081700 Rorug02G0544000 Rorug05G0089900 Rorug05G0284500 Rorug05G0452400 Rorug07G0003400 Rorug07G0003400 Rorug07G0129200 Rorug07G0210600
rosa_samantha Rh1AG193900 Rh1AG194000 Rh1DG063400 Rh1DG204700 Rh2AG364300 Rh3BG140200 Rh3CG366000 Rh4DG342600 Rh5AG347700 Rh5BG437300 Rh5CG459900 Rh5DG127400 Rh5DG412800 Rh6BG381500 Rh7CG520500 Rh7DG241900 Rh7DG336000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 526
AclWI GGATC 2 cut(s) 138, 185
AcsI RAATTY 2 cut(s) 285, 400
AfiI CCNNNNNNNGG 2 cut(s) 424, 532
AflII CTTAAG 1 cut(s) 266
AgsI TTSAA 4 cut(s) 153, 239, 374, 439
AjiI CACGTC 1 cut(s) 647
AjuI GAANNNNNNNTTGG 2 cut(s) 276, 308
AleI CACNNNNGTG 1 cut(s) 215
AluBI AGCT 2 cut(s) 22, 292
AluI AGCT 2 cut(s) 22, 292
Alw21I GWGCWC 1 cut(s) 652
Alw26I GTCTC 2 cut(s) 266, 543
AlwI GGATC 2 cut(s) 138, 185
AoxI GGCC 2 cut(s) 29, 682
ApeKI GCWGC 1 cut(s) 19
ApoI RAATTY 2 cut(s) 285, 400
ArsI GACNNNNNNTTYG 2 cut(s) 244, 276
Asp700I GAANNNNTTC 2 cut(s) 404, 521
AsuHPI GGTGA 1 cut(s) 611
BbsI GAAGAC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 652
BbvI GCAGC 1 cut(s) 31
BccI CCATC 5 cut(s) 195, 374, 465, 572, 649
BcoDI GTCTC 2 cut(s) 266, 543
BfaI CTAG 2 cut(s) 615, 642
BfrI CTTAAG 1 cut(s) 266
BisI GCNGC 1 cut(s) 20
BlsI GCNGC 1 cut(s) 21
BmgBI CACGTC 1 cut(s) 647
BmiI GGNNCC 1 cut(s) 524
BmsI GCATC 1 cut(s) 256
BpiI GAAGAC 1 cut(s) 246
Bsc4I CCNNNNNNNGG 2 cut(s) 424, 532
Bse118I RCCGGY 1 cut(s) 424
BseGI GGATG 2 cut(s) 130, 385
BseLI CCNNNNNNNGG 2 cut(s) 424, 532
BseXI GCAGC 1 cut(s) 31
BsgI GTGCAG 2 cut(s) 38, 622
BshFI GGCC 2 cut(s) 31, 684
BsiHKAI GWGCWC 1 cut(s) 652
BsiSI CCGG 1 cut(s) 425
BslI CCNNNNNNNGG 2 cut(s) 424, 532
BsmAI GTCTC 2 cut(s) 266, 543
BsnI GGCC 2 cut(s) 31, 684
Bsp1286I GDGCHC 1 cut(s) 652
Bsp143I GATC 2 cut(s) 143, 190
BspACI CCGC 1 cut(s) 526
BspANI GGCC 2 cut(s) 31, 684
BspHI TCATGA 1 cut(s) 396
BspLI GGNNCC 1 cut(s) 524
BspPI GGATC 2 cut(s) 138, 185
BspTI CTTAAG 1 cut(s) 266
BsrFI RCCGGY 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 424
BssMI GATC 2 cut(s) 143, 190
Bst4CI ACNGT 1 cut(s) 590
Bst6I CTCTTC 1 cut(s) 512
BstAFI CTTAAG 1 cut(s) 266
BstDEI CTNAG 3 cut(s) 172, 304, 545
BstF5I GGATG 2 cut(s) 130, 385
BstKTI GATC 2 cut(s) 146, 193
BstMAI GTCTC 2 cut(s) 266, 543
BstMBI GATC 2 cut(s) 143, 190
BstMWI GCNNNNNNNGC 1 cut(s) 28
BstNSI RCATGY 1 cut(s) 247
BstV1I GCAGC 1 cut(s) 31
BstV2I GAAGAC 1 cut(s) 246
BsuRI GGCC 2 cut(s) 31, 684
BtrI CACGTC 1 cut(s) 647
BtsCI GGATG 2 cut(s) 130, 385
BtsIMutI CAGTG 1 cut(s) 595
CciI TCATGA 1 cut(s) 396
Cfr10I RCCGGY 1 cut(s) 424
CviAII CATG 3 cut(s) 244, 397, 596
CviJI RGCY 8 cut(s) 22, 31, 184, 292, 366, 384, 544, 684
CviKI_1 RGCY 8 cut(s) 22, 31, 184, 292, 366, 384, 544, 684
DdeI CTNAG 3 cut(s) 172, 304, 545
DpnI GATC 2 cut(s) 145, 192
DpnII GATC 2 cut(s) 143, 190
DraI TTTAAA 2 cut(s) 61, 564
Eam1104I CTCTTC 1 cut(s) 512
EarI CTCTTC 1 cut(s) 512
Eco147I AGGCCT 1 cut(s) 684
EcoRI GAATTC 1 cut(s) 400
EcoT22I ATGCAT 1 cut(s) 249
FaeI CATG 3 cut(s) 247, 400, 599
FaiI YATR 5 cut(s) 222, 245, 398, 422, 597
FatI CATG 3 cut(s) 243, 396, 595
Fnu4HI GCNGC 1 cut(s) 20
FokI GGATG 2 cut(s) 137, 392
Fsp4HI GCNGC 1 cut(s) 20
FspBI CTAG 2 cut(s) 615, 642
GluI GCNGC 1 cut(s) 20
HaeIII GGCC 2 cut(s) 31, 684
HapII CCGG 1 cut(s) 425
Hin1II CATG 3 cut(s) 247, 400, 599
HinfI GANTC 3 cut(s) 207, 259, 271
HpaII CCGG 1 cut(s) 425
HphI GGTGA 1 cut(s) 611
Hpy166II GTNNAC 1 cut(s) 110
Hpy188I TCNGA 2 cut(s) 195, 654
Hpy188III TCNNGA 1 cut(s) 397
Hpy8I GTNNAC 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 341
HpyCH4III ACNGT 1 cut(s) 590
HpyCH4IV ACGT 2 cut(s) 36, 646
HpyCH4V TGCA 4 cut(s) 19, 44, 247, 639
HpyF10VI GCNNNNNNNGC 1 cut(s) 28
HpyF3I CTNAG 3 cut(s) 172, 304, 545
HpySE526I ACGT 2 cut(s) 36, 646
Hsp92II CATG 3 cut(s) 247, 400, 599
Kzo9I GATC 2 cut(s) 143, 190
LpnPI CCDG 2 cut(s) 198, 438
Lsp1109I GCAGC 1 cut(s) 31
LweI GCATC 1 cut(s) 256
MaeI CTAG 2 cut(s) 615, 642
MaeII ACGT 2 cut(s) 36, 646
MaeIII GTNAC 2 cut(s) 452, 599
MalI GATC 2 cut(s) 145, 192
MboI GATC 2 cut(s) 143, 190
MboII GAAGA 7 cut(s) 82, 251, 452, 523, 526, 529, 587
MhlI GDGCHC 1 cut(s) 652
MluCI AATT 4 cut(s) 285, 400, 433, 465
MlyI GAGTC 2 cut(s) 265, 268
MnlI CCTC 5 cut(s) 344, 513, 526, 661, 674
Mph1103I ATGCAT 1 cut(s) 249
MroXI GAANNNNTTC 2 cut(s) 404, 521
MseI TTAA 5 cut(s) 60, 267, 318, 432, 563
MslI CAYNNNNRTG 2 cut(s) 165, 215
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 1 cut(s) 425
MwoI GCNNNNNNNGC 1 cut(s) 28
NdeII GATC 2 cut(s) 143, 190
NlaIII CATG 3 cut(s) 247, 400, 599
NlaIV GGNNCC 1 cut(s) 524
NmuCI GTSAC 2 cut(s) 452, 599
NsiI ATGCAT 1 cut(s) 249
NspI RCATGY 1 cut(s) 247
OliI CACNNNNGTG 1 cut(s) 215
PagI TCATGA 1 cut(s) 396
PceI AGGCCT 1 cut(s) 684
PdmI GAANNNNTTC 2 cut(s) 404, 521
PfeI GAWTC 1 cut(s) 207
PkrI GCNGC 1 cut(s) 21
PleI GAGTC 2 cut(s) 265, 267
PpsI GAGTC 2 cut(s) 265, 267
PspN4I GGNNCC 1 cut(s) 524
PsrI GAACNNNNNNTAC 2 cut(s) 30, 62
RseI CAYNNNNRTG 2 cut(s) 165, 215
SaqAI TTAA 5 cut(s) 60, 267, 318, 432, 563
SatI GCNGC 1 cut(s) 20
Sau3AI GATC 2 cut(s) 143, 190
SchI GAGTC 2 cut(s) 265, 268
SduI GDGCHC 1 cut(s) 652
SetI ASST 9 cut(s) 24, 39, 217, 294, 310, 355, 524, 570, 649
SfaNI GCATC 1 cut(s) 256
SmiMI CAYNNNNRTG 2 cut(s) 165, 215
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 4 cut(s) 285, 400, 433, 465
SseBI AGGCCT 1 cut(s) 684
SsiI CCGC 1 cut(s) 526
SspI AATATT 1 cut(s) 10
SspMI CTAG 2 cut(s) 615, 642
StuI AGGCCT 1 cut(s) 684
TaaI ACNGT 1 cut(s) 590
TaiI ACGT 2 cut(s) 39, 649
TaqI TCGA 2 cut(s) 250, 531
TasI AATT 4 cut(s) 285, 400, 433, 465
TfiI GAWTC 1 cut(s) 207
Tru1I TTAA 5 cut(s) 60, 267, 318, 432, 563
Tru9I TTAA 5 cut(s) 60, 267, 318, 432, 563
TscAI CASTG 1 cut(s) 595
TseFI GTSAC 2 cut(s) 452, 599
TseI GCWGC 1 cut(s) 19
Tsp45I GTSAC 2 cut(s) 452, 599
TspDTI ATGAA 2 cut(s) 385, 413
TspGWI ACGGA 2 cut(s) 285, 523
TspRI CASTG 1 cut(s) 595
Vha464I CTTAAG 1 cut(s) 266
XapI RAATTY 2 cut(s) 285, 400
XceI RCATGY 1 cut(s) 247
XmnI GAANNNNTTC 2 cut(s) 404, 521
XspI CTAG 2 cut(s) 615, 642
Zsp2I ATGCAT 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.