Rmu_sc0004924.1_g000016

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004924.1
OG0000619
Physical Location & Seq
Forward (+)
59522 .. 61231
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004924.1_g000016.1.cds

Sequence Viewer

Length: 1272 bp
ctgaattcctattcaaataaccatattgatgtcttggttggcaagcgcacggaccctgacaggtggagatttactggggtgtatggttaccctaaggtggaagataggcacttgacgtggaatcttctgaggtttcttgggcaacaaaatctgtggccttgggttctgggtggagactttaacgaaatcgtctctgctttggaaaaggaaggtgaactggtgaggcgtgaacataagatggatgggtttcgagactttatgcatgattttgggttgcaccatgttccttggtctggtcctagttttacttggagtggagttagaggtggctgcgtagttaaagttaggttagatagatttattgcttctcagcattggttagataaattcctcctatctagggcctacaatttaaaccctagtaggagtgaccatcttcctattttgttggaagtcaagaaaaaacgcaaaaagaagaggaggaaacggaagaagagattccggtttgaggaggtgtggcttcgggatgaggagtgtctgaaggttgtggaggacggatggcagcgcggtagaggcccggatccgttttcccgagtttgtaacaaactggactgtactaggcaggcactagttttgtggagcgaagaaaagttcaatggtcttaagaagaggatcgaggatgtcaggggacgactagccatcttttttgacacttctctttcagctccgttagatgatgtcaagagggctggtaacagaaggaggaagaaccatatcaagggtcttttcaatgaagccaatagttggtgtactgaggatgaggatttggaggctatcatcttgtcttattatggagagctttttacgacctcacagcctacaaactttgaccattttttaacggttatcccaaggcttgtttcacaggggatgaatgacgagttaacgaaagtaatatctatggaggaagtgtatggtgctcttcggcagatgcatccatccaaatcgcctggacaggatggctttgccccgtgcttctaccaacatttttggggtgtggttggtaatgatgtgttggaggctggtttgctaaagcgtgactttagcttcgctgggttgcgaggacgttactcttgcttcactggtagtatcagtggcagtagcactggcagtcggctcgcggggagactaagactggaagtacggtcgccgggtttcaacgaggttgaaggtttgctccggggaggctttgtaatcgttgggattgattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

423

Amino Acids

48.85

Weight (kDa)

9.39

Isoelectric Point (pI)

50.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000619)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29861 FvH4_3g26084 FvH4_3g32551 FvH4_4g08193 FvH4_4g12950 FvH4_6g29733
rosa_chinensis RchiOBHm_Chr3g0488921
rosa_multiflora Rmu_co8238041.1_g000001 Rmu_co8265215.1_g000001 Rmu_co8486357.1_g000002 Rmu_sc0000389.1_g000016 Rmu_sc0000580.1_g000123 Rmu_sc0000789.1_g000005 Rmu_sc0001043.1_g000006 Rmu_sc0001208.1_g000001 Rmu_sc0001323.1_g000011 Rmu_sc0001636.1_g000001 Rmu_sc0001911.1_g000023 Rmu_sc0001940.1_g000010 Rmu_sc0002531.1_g000047 Rmu_sc0002715.1_g000007 Rmu_sc0002833.1_g000024 Rmu_sc0003859.1_g000046 Rmu_sc0003894.1_g000008 Rmu_sc0004744.1_g000008 Rmu_sc0004924.1_g000016 Rmu_sc0004991.1_g000004 Rmu_sc0005149.1_g000010 Rmu_sc0005149.1_g000011 Rmu_sc0005489.1_g000007 Rmu_sc0005592.1_g000030 Rmu_sc0005813.1_g000010 Rmu_sc0005813.1_g000011 Rmu_sc0005861.1_g000009 Rmu_sc0005949.1_g000016 Rmu_sc0006824.1_g000021 Rmu_sc0007069.1_g000025 Rmu_sc0008002.1_g000008 Rmu_sc0008148.1_g000008 Rmu_sc0008280.1_g000001 Rmu_sc0008317.1_g000010 Rmu_sc0011615.1_g000002 Rmu_sc0013925.1_g000004 Rmu_sc0014130.1_g000003 Rmu_sc0014510.1_g000008 Rmu_sc0016640.1_g000001 Rmu_sc0018068.1_g000002 Rmu_sc0021371.1_g000006 Rmu_sc0028652.1_g000007 Rmu_sc0032187.1_g000002 Rmu_sc0034241.1_g000001 Rmu_ssc0000127.1_g000003
rosa_roxburghii Rroxscaffold_6G00403950
rosa_rugosa Rorug01G0081700 Rorug02G0544000 Rorug05G0089900 Rorug05G0284500 Rorug05G0452400 Rorug07G0003400 Rorug07G0003400 Rorug07G0129200 Rorug07G0210600
rosa_samantha Rh1AG193900 Rh1AG194000 Rh1DG063400 Rh1DG204700 Rh2AG364300 Rh3BG140200 Rh3CG366000 Rh4DG342600 Rh5AG347700 Rh5BG437300 Rh5CG459900 Rh5DG127400 Rh5DG412800 Rh6BG381500 Rh7CG520500 Rh7DG241900 Rh7DG336000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 804
AccII CGCG 2 cut(s) 567, 1181
AciI CCGC 2 cut(s) 567, 1181
AclWI GGATC 3 cut(s) 575, 588, 680
AcsI RAATTY 2 cut(s) 4, 386
AcuI CTGAAG 1 cut(s) 560
AfaI GTAC 3 cut(s) 616, 811, 1203
AfiI CCNNNNNNNGG 6 cut(s) 97, 293, 400, 508, 778, 804
AflII CTTAAG 1 cut(s) 662
AgsI TTSAA 5 cut(s) 15, 655, 790, 1219, 1229
AhlI ACTAGT 1 cut(s) 628
AjiI CACGTC 1 cut(s) 117
AjnI CCWGG 1 cut(s) 1009
AjuI GAANNNNNNNTTGG 2 cut(s) 904, 936
AluBI AGCT 3 cut(s) 725, 859, 1107
AluI AGCT 3 cut(s) 725, 859, 1107
Alw21I GWGCWC 1 cut(s) 982
Alw26I GTCTC 4 cut(s) 168, 196, 246, 1180
AlwI GGATC 3 cut(s) 575, 588, 680
Ama87I CYCGRG 1 cut(s) 591
AoxI GGCC 3 cut(s) 155, 402, 574
ApeKI GCWGC 2 cut(s) 330, 562
ApoI RAATTY 2 cut(s) 4, 386
ArsI GACNNNNNNTTYG 2 cut(s) 1091, 1123
Asp700I GAANNNNTTC 1 cut(s) 497
AspLEI GCGC 2 cut(s) 48, 567
AspS9I GGNCC 4 cut(s) 52, 296, 402, 575
AsuC2I CCSGG 3 cut(s) 578, 1212, 1241
AsuHPI GGTGA 2 cut(s) 224, 232
AvaI CYCGRG 1 cut(s) 591
AvaII GGWCC 2 cut(s) 52, 296
AxyI CCTNAGG 1 cut(s) 93
BamHI GGATCC 1 cut(s) 580
Bbv12I GWGCWC 1 cut(s) 982
BbvI GCAGC 2 cut(s) 317, 574
BccI CCATC 7 cut(s) 232, 236, 441, 552, 707, 1006, 1013
BcgI CGANNNNNNTGC 2 cut(s) 1159, 1193
BciT130I CCWGG 1 cut(s) 1011
BcnI CCSGG 3 cut(s) 578, 1212, 1241
BcoDI GTCTC 4 cut(s) 168, 196, 246, 1180
BcuI ACTAGT 1 cut(s) 628
BfaI CTAG 6 cut(s) 300, 399, 420, 618, 629, 695
BfrI CTTAAG 1 cut(s) 662
BisI GCNGC 2 cut(s) 331, 563
BlsI GCNGC 2 cut(s) 332, 564
Bme1390I CCNGG 4 cut(s) 578, 1011, 1212, 1241
Bme18I GGWCC 2 cut(s) 52, 296
BmeT110I CYCGRG 1 cut(s) 591
BmgBI CACGTC 1 cut(s) 117
BmgT120I GGNCC 4 cut(s) 52, 296, 402, 575
BmiI GGNNCC 2 cut(s) 54, 582
BmrFI CCNGG 4 cut(s) 578, 1011, 1212, 1241
BmrI ACTGGG 1 cut(s) 84
BmsI GCATC 2 cut(s) 981, 1003
BmuI ACTGGG 1 cut(s) 84
BpuMI CCSGG 3 cut(s) 578, 1212, 1241
BsaJI CCNNGG 4 cut(s) 158, 287, 911, 1240
BsaWI WCCGGW 1 cut(s) 501
BsaXI ACNNNNNCTCC 4 cut(s) 956, 986, 1236, 1266
Bsc4I CCNNNNNNNGG 6 cut(s) 97, 293, 400, 508, 778, 804
Bse1I ACTGG 6 cut(s) 79, 222, 612, 1147, 1171, 1200
Bse21I CCTNAGG 1 cut(s) 93
BseBI CCWGG 1 cut(s) 1011
BseDI CCNNGG 4 cut(s) 158, 287, 911, 1240
BseGI GGATG 9 cut(s) 247, 532, 563, 685, 823, 936, 994, 998, 1024
BseLI CCNNNNNNNGG 6 cut(s) 97, 293, 400, 508, 778, 804
BseMII CTCAG 3 cut(s) 119, 383, 804
BseNI ACTGG 6 cut(s) 79, 222, 612, 1147, 1171, 1200
BseRI GAGGAG 3 cut(s) 493, 524, 545
BseXI GCAGC 2 cut(s) 317, 574
BseYI CCCAGC 1 cut(s) 1112
Bsh1236I CGCG 2 cut(s) 567, 1181
Bsh1285I CGRYCG 1 cut(s) 1208
BshFI GGCC 3 cut(s) 157, 404, 576
BsiEI CGRYCG 1 cut(s) 1208
BsiHKAI GWGCWC 1 cut(s) 982
BsiHKCI CYCGRG 1 cut(s) 591
BsiSI CCGG 4 cut(s) 502, 578, 1211, 1240
BslFI GGGAC 1 cut(s) 702
BslI CCNNNNNNNGG 6 cut(s) 97, 293, 400, 508, 778, 804
BsmAI GTCTC 4 cut(s) 168, 196, 246, 1180
BsmBI CGTCTC 1 cut(s) 196
BsmFI GGGAC 1 cut(s) 702
BsnI GGCC 3 cut(s) 157, 404, 576
BsoBI CYCGRG 1 cut(s) 591
Bsp1286I GDGCHC 1 cut(s) 982
Bsp143I GATC 2 cut(s) 580, 672
BspACI CCGC 2 cut(s) 567, 1181
BspANI GGCC 3 cut(s) 157, 404, 576
BspCNI CTCAG 3 cut(s) 120, 382, 805
BspFNI CGCG 2 cut(s) 567, 1181
BspLI GGNNCC 2 cut(s) 54, 582
BspPI GGATC 3 cut(s) 575, 588, 680
BspQI GCTCTTC 1 cut(s) 987
BspTI CTTAAG 1 cut(s) 662
BsrI ACTGG 6 cut(s) 79, 222, 612, 1147, 1171, 1200
BssECI CCNNGG 4 cut(s) 158, 287, 911, 1240
BssMI GATC 2 cut(s) 580, 672
BssT1I CCWWGG 3 cut(s) 158, 287, 911
Bst2UI CCWGG 1 cut(s) 1011
Bst4CI ACNGT 3 cut(s) 614, 904, 1206
Bst6I CTCTTC 4 cut(s) 470, 488, 662, 987
BstAFI CTTAAG 1 cut(s) 662
BstC8I GCNNGC 3 cut(s) 44, 624, 1179
BstDEI CTNAG 5 cut(s) 93, 128, 369, 813, 1190
BstEII GGTNACC 1 cut(s) 86
BstF5I GGATG 9 cut(s) 247, 532, 563, 685, 823, 936, 994, 998, 1024
BstFNI CGCG 2 cut(s) 567, 1181
BstHHI GCGC 2 cut(s) 48, 567
BstKTI GATC 2 cut(s) 583, 675
BstMAI GTCTC 4 cut(s) 168, 196, 246, 1180
BstMBI GATC 2 cut(s) 580, 672
BstMCI CGRYCG 1 cut(s) 1208
BstMWI GCNNNNNNNGC 1 cut(s) 573
BstNI CCWGG 1 cut(s) 1011
BstPI GGTNACC 1 cut(s) 86
BstSCI CCNGG 4 cut(s) 576, 1009, 1210, 1239
BstUI CGCG 2 cut(s) 567, 1181
BstV1I GCAGC 2 cut(s) 317, 574
BstX2I RGATCY 1 cut(s) 580
BstYI RGATCY 1 cut(s) 580
Bsu36I CCTNAGG 1 cut(s) 93
BsuRI GGCC 3 cut(s) 157, 404, 576
BtrI CACGTC 1 cut(s) 117
BtsCI GGATG 9 cut(s) 247, 532, 563, 685, 823, 936, 994, 998, 1024
BtsIMutI CAGTG 3 cut(s) 1140, 1159, 1164
Cac8I GCNNGC 3 cut(s) 44, 624, 1179
CfoI GCGC 2 cut(s) 48, 567
Cfr13I GGNCC 4 cut(s) 52, 296, 402, 575
Csp6I GTAC 3 cut(s) 615, 810, 1202
CspCI CAANNNNNGTGG 2 cut(s) 134, 169
CviAII CATG 2 cut(s) 263, 281
CviQI GTAC 3 cut(s) 615, 810, 1202
DdeI CTNAG 5 cut(s) 93, 128, 369, 813, 1190
DpnI GATC 2 cut(s) 582, 674
DpnII GATC 2 cut(s) 580, 672
DraI TTTAAA 1 cut(s) 414
Eam1104I CTCTTC 4 cut(s) 470, 488, 662, 987
EarI CTCTTC 4 cut(s) 470, 488, 662, 987
Eco130I CCWWGG 3 cut(s) 158, 287, 911
Eco47I GGWCC 2 cut(s) 52, 296
Eco57I CTGAAG 1 cut(s) 560
Eco81I CCTNAGG 1 cut(s) 93
Eco88I CYCGRG 1 cut(s) 591
Eco91I GGTNACC 1 cut(s) 86
EcoO109I RGGNCCY 1 cut(s) 402
EcoO65I GGTNACC 1 cut(s) 86
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 1009
EcoT14I CCWWGG 3 cut(s) 158, 287, 911
EcoT22I ATGCAT 2 cut(s) 264, 996
ErhI CCWWGG 3 cut(s) 158, 287, 911
Esp3I CGTCTC 1 cut(s) 196
FaeI CATG 2 cut(s) 266, 284
FalI AAGNNNNNCTT 2 cut(s) 1085, 1117
FaqI GGGAC 1 cut(s) 702
FatI CATG 2 cut(s) 262, 280
FauI CCCGC 1 cut(s) 1174
Fnu4HI GCNGC 2 cut(s) 331, 563
FokI GGATG 9 cut(s) 254, 539, 570, 692, 830, 943, 981, 985, 1031
Fsp4HI GCNGC 2 cut(s) 331, 563
FspBI CTAG 6 cut(s) 300, 399, 420, 618, 629, 695
GlaI GCGC 2 cut(s) 47, 566
GluI GCNGC 2 cut(s) 331, 563
GsaI CCCAGC 1 cut(s) 1116
HaeIII GGCC 3 cut(s) 157, 404, 576
HapII CCGG 4 cut(s) 502, 578, 1211, 1240
HhaI GCGC 2 cut(s) 48, 567
Hin1II CATG 2 cut(s) 266, 284
Hin6I GCGC 2 cut(s) 46, 565
HinP1I GCGC 2 cut(s) 46, 565
HincII GTYRAC 1 cut(s) 945
HindII GTYRAC 1 cut(s) 945
HinfI GANTC 2 cut(s) 121, 498
HpaI GTTAAC 1 cut(s) 945
HpaII CCGG 4 cut(s) 502, 578, 1211, 1240
HphI GGTGA 2 cut(s) 224, 232
Hpy166II GTNNAC 4 cut(s) 215, 230, 810, 945
Hpy188I TCNGA 2 cut(s) 129, 540
Hpy188III TCNNGA 5 cut(s) 251, 457, 524, 591, 742
Hpy8I GTNNAC 4 cut(s) 215, 230, 810, 945
HpyAV CCTTC 4 cut(s) 203, 535, 753, 1223
HpyCH4III ACNGT 3 cut(s) 614, 904, 1206
HpyCH4IV ACGT 2 cut(s) 116, 1126
HpyCH4V TGCA 3 cut(s) 262, 277, 994
HpyF10VI GCNNNNNNNGC 1 cut(s) 573
HpyF3I CTNAG 5 cut(s) 93, 128, 369, 813, 1190
HpySE526I ACGT 2 cut(s) 116, 1126
Hsp92II CATG 2 cut(s) 266, 284
HspAI GCGC 2 cut(s) 46, 565
KspAI GTTAAC 1 cut(s) 945
Kzo9I GATC 2 cut(s) 580, 672
LguI GCTCTTC 1 cut(s) 987
LmnI GCTCC 3 cut(s) 639, 730, 1242
Lsp1109I GCAGC 2 cut(s) 317, 574
LweI GCATC 2 cut(s) 981, 1003
MaeI CTAG 6 cut(s) 300, 399, 420, 618, 629, 695
MaeII ACGT 2 cut(s) 116, 1126
MaeIII GTNAC 6 cut(s) 86, 428, 599, 752, 1097, 1127
MalI GATC 2 cut(s) 582, 674
MboI GATC 2 cut(s) 580, 672
MflI RGATCY 1 cut(s) 580
MhlI GDGCHC 1 cut(s) 982
MluCI AATT 3 cut(s) 4, 386, 409
MmeI TCCRAC 2 cut(s) 429, 1056
Mph1103I ATGCAT 2 cut(s) 264, 996
MroXI GAANNNNTTC 1 cut(s) 497
MseI TTAA 6 cut(s) 180, 339, 413, 663, 899, 944
MslI CAYNNNNRTG 1 cut(s) 27
MspCI CTTAAG 1 cut(s) 662
MspI CCGG 4 cut(s) 502, 578, 1211, 1240
MspR9I CCNGG 4 cut(s) 578, 1011, 1212, 1241
MvaI CCWGG 1 cut(s) 1011
MvnI CGCG 2 cut(s) 567, 1181
MwoI GCNNNNNNNGC 1 cut(s) 573
NciI CCSGG 3 cut(s) 578, 1212, 1241
NdeII GATC 2 cut(s) 580, 672
NlaIII CATG 2 cut(s) 266, 284
NlaIV GGNNCC 2 cut(s) 54, 582
NmuCI GTSAC 2 cut(s) 428, 1097
NsiI ATGCAT 2 cut(s) 264, 996
PciSI GCTCTTC 1 cut(s) 987
PdmI GAANNNNTTC 1 cut(s) 497
PfeI GAWTC 2 cut(s) 121, 498
PflMI CCANNNNNTGG 1 cut(s) 804
PkrI GCNGC 2 cut(s) 332, 564
Psp6I CCWGG 1 cut(s) 1009
PspEI GGTNACC 1 cut(s) 86
PspFI CCCAGC 1 cut(s) 1112
PspGI CCWGG 1 cut(s) 1009
PspN4I GGNNCC 2 cut(s) 54, 582
PspPI GGNCC 4 cut(s) 52, 296, 402, 575
PsuI RGATCY 1 cut(s) 580
RsaI GTAC 3 cut(s) 616, 811, 1203
RsaNI GTAC 3 cut(s) 615, 810, 1202
RseI CAYNNNNRTG 1 cut(s) 27
SapI GCTCTTC 1 cut(s) 987
SaqAI TTAA 6 cut(s) 180, 339, 413, 663, 899, 944
SatI GCNGC 2 cut(s) 331, 563
Sau3AI GATC 2 cut(s) 580, 672
Sau96I GGNCC 4 cut(s) 52, 296, 402, 575
ScrFI CCNGG 4 cut(s) 578, 1011, 1212, 1241
SduI GDGCHC 1 cut(s) 982
SfaNI GCATC 2 cut(s) 981, 1003
SinI GGWCC 2 cut(s) 52, 296
SmiMI CAYNNNNRTG 1 cut(s) 27
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
SpeI ACTAGT 1 cut(s) 628
Sse9I AATT 3 cut(s) 4, 386, 409
SsiI CCGC 2 cut(s) 567, 1181
SspMI CTAG 6 cut(s) 300, 399, 420, 618, 629, 695
StyD4I CCNGG 4 cut(s) 576, 1009, 1210, 1239
StyI CCWWGG 3 cut(s) 158, 287, 911
TaaI ACNGT 3 cut(s) 614, 904, 1206
TaiI ACGT 2 cut(s) 119, 1129
TaqI TCGA 2 cut(s) 250, 675
TasI AATT 3 cut(s) 4, 386, 409
TatI WGTACW 2 cut(s) 614, 809
TfiI GAWTC 2 cut(s) 121, 498
Tru1I TTAA 6 cut(s) 180, 339, 413, 663, 899, 944
Tru9I TTAA 6 cut(s) 180, 339, 413, 663, 899, 944
TscAI CASTG 3 cut(s) 1147, 1159, 1171
TseFI GTSAC 2 cut(s) 428, 1097
TseI GCWGC 2 cut(s) 330, 562
Tsp45I GTSAC 2 cut(s) 428, 1097
TspDTI ATGAA 2 cut(s) 807, 947
TspGWI ACGGA 5 cut(s) 65, 502, 570, 573, 717
TspRI CASTG 3 cut(s) 1147, 1159, 1171
Van91I CCANNNNNTGG 1 cut(s) 804
Vha464I CTTAAG 1 cut(s) 662
VpaK11BI GGWCC 2 cut(s) 52, 296
XapI RAATTY 2 cut(s) 4, 386
XmnI GAANNNNTTC 1 cut(s) 497
XspI CTAG 6 cut(s) 300, 399, 420, 618, 629, 695
Zsp2I ATGCAT 2 cut(s) 264, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.