Rh5BG437300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000619
Physical Location & Seq
Forward (+)
70439407 .. 70454755
15349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG437300.1

Sequence Viewer

Length: 558 bp
ATGACTTCGGGTTTGGAAACTACTTTGGGAATAGATTCAGTCATTGATAGTGGATCAGATCTGAAACCAGCATTGATGGCTAATGGGTCTAGTTTTGGAATGCTTGCTCTGAGTTCGATCTTAGGAAGGATGTGGACTGATTTGGGCTCCCAGAGTTTGGAGCATGATTGTAGTCTCCCATCATTGGGGGCGGAAAGTAAGATCAAGAAAACCGAGTTAACTTTGGAGGGGTCCTTAGATTTGGGGACAGCTGGCAGCCATCATGATGGTAATTCTGTATCTTTGGGGGATGGAGATGGGCTTTCTCTAACAGGGTCTGATCATGGGCTCCTAACTAAATCTCAAGAGACTGGGCTTGAGACAAGTGATTCTGCAAATGGGGCTGTGACAATTCACTCTGGGCCCTTTATTGCCCGACGTAGAGAGAAAGAGAAGTGCGCCGATCAGGTCTTCCACAAGCGGAAGGAGTTTCTAGGTCTTTTACTTTTCCACTTCTACCAGAAGCCTGTACTATTATTTATGTCTTCTTCGATGATGATTGGTTTAAATTCTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

19.55

Weight (kDa)

5.41

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000619)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29861 FvH4_3g26084 FvH4_3g32551 FvH4_4g08193 FvH4_4g12950 FvH4_6g29733
rosa_chinensis RchiOBHm_Chr3g0488921
rosa_multiflora Rmu_co8238041.1_g000001 Rmu_co8265215.1_g000001 Rmu_co8486357.1_g000002 Rmu_sc0000389.1_g000016 Rmu_sc0000580.1_g000123 Rmu_sc0000789.1_g000005 Rmu_sc0001043.1_g000006 Rmu_sc0001208.1_g000001 Rmu_sc0001323.1_g000011 Rmu_sc0001636.1_g000001 Rmu_sc0001911.1_g000023 Rmu_sc0001940.1_g000010 Rmu_sc0002531.1_g000047 Rmu_sc0002715.1_g000007 Rmu_sc0002833.1_g000024 Rmu_sc0003859.1_g000046 Rmu_sc0003894.1_g000008 Rmu_sc0004744.1_g000008 Rmu_sc0004924.1_g000016 Rmu_sc0004991.1_g000004 Rmu_sc0005149.1_g000010 Rmu_sc0005149.1_g000011 Rmu_sc0005489.1_g000007 Rmu_sc0005592.1_g000030 Rmu_sc0005813.1_g000010 Rmu_sc0005813.1_g000011 Rmu_sc0005861.1_g000009 Rmu_sc0005949.1_g000016 Rmu_sc0006824.1_g000021 Rmu_sc0007069.1_g000025 Rmu_sc0008002.1_g000008 Rmu_sc0008148.1_g000008 Rmu_sc0008280.1_g000001 Rmu_sc0008317.1_g000010 Rmu_sc0011615.1_g000002 Rmu_sc0013925.1_g000004 Rmu_sc0014130.1_g000003 Rmu_sc0014510.1_g000008 Rmu_sc0016640.1_g000001 Rmu_sc0018068.1_g000002 Rmu_sc0021371.1_g000006 Rmu_sc0028652.1_g000007 Rmu_sc0032187.1_g000002 Rmu_sc0034241.1_g000001 Rmu_ssc0000127.1_g000003
rosa_roxburghii Rroxscaffold_6G00403950
rosa_rugosa Rorug01G0081700 Rorug02G0544000 Rorug05G0089900 Rorug05G0284500 Rorug05G0452400 Rorug07G0003400 Rorug07G0003400 Rorug07G0129200 Rorug07G0210600
rosa_samantha Rh1AG193900 Rh1AG194000 Rh1DG063400 Rh1DG204700 Rh2AG364300 Rh3BG140200 Rh3CG366000 Rh4DG342600 Rh5AG347700 Rh5BG437300 Rh5CG459900 Rh5DG127400 Rh5DG412800 Rh6BG381500 Rh7CG520500 Rh7DG241900 Rh7DG336000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 157
AciI CCGC 2 cut(s) 191, 460
AclWI GGATC 1 cut(s) 61
AcsI RAATTY 1 cut(s) 547
AfaI GTAC 1 cut(s) 510
AfiI CCNNNNNNNGG 3 cut(s) 157, 184, 185
AluBI AGCT 1 cut(s) 251
AluI AGCT 1 cut(s) 251
Alw26I GTCTC 3 cut(s) 179, 341, 353
AlwI GGATC 1 cut(s) 61
AlwNI CAGNNNCTG 1 cut(s) 317
AoxI GGCC 1 cut(s) 401
ApaI GGGCCC 1 cut(s) 405
ApeKI GCWGC 1 cut(s) 255
ApoI RAATTY 1 cut(s) 547
ArsI GACNNNNNNTTYG 1 cut(s) 27
Asp700I GAANNNNTTC 1 cut(s) 34
AspLEI GCGC 1 cut(s) 440
AspS9I GGNCC 3 cut(s) 231, 401, 402
AvaII GGWCC 1 cut(s) 231
BaeGI GKGCMC 1 cut(s) 405
BanII GRGCYC 3 cut(s) 149, 330, 405
BbsI GAAGAC 2 cut(s) 442, 516
BbvI GCAGC 1 cut(s) 267
BccI CCATC 6 cut(s) 70, 187, 260, 267, 284, 290
BclI TGATCA 1 cut(s) 319
BcoDI GTCTC 3 cut(s) 179, 341, 353
BfaI CTAG 2 cut(s) 90, 473
BglII AGATCT 1 cut(s) 58
BisI GCNGC 1 cut(s) 256
BlsI GCNGC 1 cut(s) 257
Bme18I GGWCC 1 cut(s) 231
BmgT120I GGNCC 3 cut(s) 231, 401, 402
BmiI GGNNCC 4 cut(s) 148, 232, 329, 403
BmrI ACTGGG 1 cut(s) 360
BmuI ACTGGG 1 cut(s) 360
BpiI GAAGAC 2 cut(s) 442, 516
BpuEI CTTGAG 2 cut(s) 327, 377
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 184, 185
Bse1I ACTGG 1 cut(s) 355
BseGI GGATG 2 cut(s) 135, 295
BseLI CCNNNNNNNGG 3 cut(s) 157, 184, 185
BseMII CTCAG 1 cut(s) 101
BseNI ACTGG 1 cut(s) 355
BseSI GKGCMC 1 cut(s) 405
BseXI GCAGC 1 cut(s) 267
BshFI GGCC 1 cut(s) 403
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 3 cut(s) 157, 184, 185
BsmAI GTCTC 3 cut(s) 179, 341, 353
BsmFI GGGAC 1 cut(s) 259
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 1 cut(s) 403
Bsp120I GGGCCC 1 cut(s) 401
Bsp1286I GDGCHC 3 cut(s) 149, 330, 405
Bsp143I GATC 6 cut(s) 53, 58, 117, 201, 319, 442
BspACI CCGC 2 cut(s) 191, 460
BspANI GGCC 1 cut(s) 403
BspCNI CTCAG 1 cut(s) 102
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 4 cut(s) 148, 232, 329, 403
BspPI GGATC 1 cut(s) 61
BsrI ACTGG 1 cut(s) 355
BssMI GATC 6 cut(s) 53, 58, 117, 201, 319, 442
BstC8I GCNNGC 2 cut(s) 105, 253
BstDEI CTNAG 3 cut(s) 110, 121, 235
BstF5I GGATG 2 cut(s) 135, 295
BstHHI GCGC 1 cut(s) 440
BstKTI GATC 6 cut(s) 56, 61, 120, 204, 322, 445
BstMAI GTCTC 3 cut(s) 179, 341, 353
BstMBI GATC 6 cut(s) 53, 58, 117, 201, 319, 442
BstMWI GCNNNNNNNGC 2 cut(s) 77, 380
BstSLI GKGCMC 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 267
BstV2I GAAGAC 2 cut(s) 442, 516
BstX2I RGATCY 1 cut(s) 58
BstXI CCANNNNNNTGG 1 cut(s) 266
BstYI RGATCY 1 cut(s) 58
BsuRI GGCC 1 cut(s) 403
BtsCI GGATG 2 cut(s) 135, 295
Cac8I GCNNGC 2 cut(s) 105, 253
CaiI CAGNNNCTG 1 cut(s) 317
CciI TCATGA 1 cut(s) 262
CfoI GCGC 1 cut(s) 440
Cfr13I GGNCC 3 cut(s) 231, 401, 402
Csp6I GTAC 1 cut(s) 509
CviAII CATG 3 cut(s) 164, 263, 323
CviQI GTAC 1 cut(s) 509
DdeI CTNAG 3 cut(s) 110, 121, 235
DpnI GATC 6 cut(s) 55, 60, 119, 203, 321, 444
DpnII GATC 6 cut(s) 53, 58, 117, 201, 319, 442
DraI TTTAAA 1 cut(s) 546
EciI GGCGGA 1 cut(s) 206
Eco24I GRGCYC 3 cut(s) 149, 330, 405
Eco47I GGWCC 1 cut(s) 231
EcoO109I RGGNCCY 2 cut(s) 231, 402
EcoT38I GRGCYC 3 cut(s) 149, 330, 405
FaeI CATG 3 cut(s) 167, 266, 326
FaiI YATR 4 cut(s) 165, 264, 324, 521
FaqI GGGAC 1 cut(s) 259
FatI CATG 3 cut(s) 163, 262, 322
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 1 cut(s) 256
FokI GGATG 2 cut(s) 142, 302
FriOI GRGCYC 3 cut(s) 149, 330, 405
Fsp4HI GCNGC 1 cut(s) 256
FspBI CTAG 2 cut(s) 90, 473
GlaI GCGC 1 cut(s) 439
GluI GCNGC 1 cut(s) 256
HaeIII GGCC 1 cut(s) 403
HhaI GCGC 1 cut(s) 440
Hin1II CATG 3 cut(s) 167, 266, 326
Hin6I GCGC 1 cut(s) 438
HinP1I GCGC 1 cut(s) 438
HincII GTYRAC 1 cut(s) 219
HindII GTYRAC 1 cut(s) 219
HinfI GANTC 2 cut(s) 35, 368
HpaI GTTAAC 1 cut(s) 219
Hpy166II GTNNAC 2 cut(s) 135, 219
Hpy188I TCNGA 4 cut(s) 58, 63, 111, 319
Hpy188III TCNNGA 3 cut(s) 205, 263, 344
Hpy8I GTNNAC 2 cut(s) 135, 219
Hpy99I CGWCG 1 cut(s) 420
HpyAV CCTTC 2 cut(s) 120, 457
HpyCH4IV ACGT 1 cut(s) 418
HpyCH4V TGCA 1 cut(s) 374
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 380
HpyF3I CTNAG 3 cut(s) 110, 121, 235
HpySE526I ACGT 1 cut(s) 418
Hsp92II CATG 3 cut(s) 167, 266, 326
HspAI GCGC 1 cut(s) 438
Ksp22I TGATCA 1 cut(s) 319
KspAI GTTAAC 1 cut(s) 219
Kzo9I GATC 6 cut(s) 53, 58, 117, 201, 319, 442
LmnI GCTCC 3 cut(s) 152, 160, 333
Lsp1109I GCAGC 1 cut(s) 267
MaeI CTAG 2 cut(s) 90, 473
MaeII ACGT 1 cut(s) 418
MaeIII GTNAC 1 cut(s) 385
MalI GATC 6 cut(s) 55, 60, 119, 203, 321, 444
MboI GATC 6 cut(s) 53, 58, 117, 201, 319, 442
MboII GAAGA 3 cut(s) 442, 516, 519
MflI RGATCY 1 cut(s) 58
MhlI GDGCHC 3 cut(s) 149, 330, 405
MluCI AATT 3 cut(s) 271, 390, 547
MnlI CCTC 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 34
MseI TTAA 2 cut(s) 218, 545
MslI CAYNNNNRTG 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 251
Mva1269I GAATGC 1 cut(s) 105
MwoI GCNNNNNNNGC 2 cut(s) 77, 380
NdeII GATC 6 cut(s) 53, 58, 117, 201, 319, 442
NlaIII CATG 3 cut(s) 167, 266, 326
NlaIV GGNNCC 4 cut(s) 148, 232, 329, 403
NmuCI GTSAC 1 cut(s) 385
PagI TCATGA 1 cut(s) 262
PctI GAATGC 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 34
PfeI GAWTC 2 cut(s) 35, 368
PflMI CCANNNNNTGG 1 cut(s) 157
PkrI GCNGC 1 cut(s) 257
PpuMI RGGWCCY 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 231
PspN4I GGNNCC 4 cut(s) 148, 232, 329, 403
PspOMI GGGCCC 1 cut(s) 401
PspPI GGNCC 3 cut(s) 231, 401, 402
PspPPI RGGWCCY 1 cut(s) 231
PstNI CAGNNNCTG 1 cut(s) 317
PsuI RGATCY 1 cut(s) 58
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 1 cut(s) 510
RsaNI GTAC 1 cut(s) 509
RseI CAYNNNNRTG 1 cut(s) 264
SaqAI TTAA 2 cut(s) 218, 545
SatI GCNGC 1 cut(s) 256
Sau3AI GATC 6 cut(s) 53, 58, 117, 201, 319, 442
Sau96I GGNCC 3 cut(s) 231, 401, 402
SduI GDGCHC 3 cut(s) 149, 330, 405
SetI ASST 4 cut(s) 253, 421, 450, 478
SinI GGWCC 1 cut(s) 231
SmiMI CAYNNNNRTG 1 cut(s) 264
SmlI CTYRAG 2 cut(s) 342, 356
SmoI CTYRAG 2 cut(s) 342, 356
Sse9I AATT 3 cut(s) 271, 390, 547
SsiI CCGC 2 cut(s) 191, 460
SspMI CTAG 2 cut(s) 90, 473
TaiI ACGT 1 cut(s) 421
TaqI TCGA 2 cut(s) 116, 530
TasI AATT 3 cut(s) 271, 390, 547
TatI WGTACW 1 cut(s) 508
TfiI GAWTC 2 cut(s) 35, 368
Tru1I TTAA 2 cut(s) 218, 545
Tru9I TTAA 2 cut(s) 218, 545
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 1 cut(s) 255
Tsp45I GTSAC 1 cut(s) 385
Van91I CCANNNNNTGG 1 cut(s) 157
VpaK11BI GGWCC 1 cut(s) 231
XapI RAATTY 1 cut(s) 547
XmnI GAANNNNTTC 1 cut(s) 34
XspI CTAG 2 cut(s) 90, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.