Rorug05G0284500

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000619
Physical Location & Seq
Forward (+)
32626301 .. 32627845
1545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0284500.1

Sequence Viewer

Length: 1287 bp
ATGGCCTCTATTCAAGCTTCTTTTTGTTCTGCTTCATTTTCTTCAGCTTCCTCTAGAAAAATCAAAGCTACTCTCCACTCCCAAAACATTGAATCATCCCGTCTTTCAGTTCCCAGGCTCCTGGTTAATGAACTGAATCAAAGAAATGGGTCACTCAACCTAACAGCCACTTCATCTGAAATAGAAGTGAAACCATTATTTGACAACAGAAAAAGATCAAATCTTGGTGACTCAGTTTCAGATTCAAACTTTGTCCAAGAGCTCTACGCAATAATGGAAATTGTGGCAGATAGAATAGAAATGCACAACAACATAAAGGCTCAGAGGGACAACTGGAATGTTCTGCTTCTCAACTCTGTGAATGGGATGACAGCCACAGCTGCAATCATGGCGGGACTTGCAGCTGTCTCTGGATCTGGACCGTCCATTTTGTCTCTCAAGATCTCATCAAGTCTCCTATACATGGCTGTGACTGGGATTGTTCTAGTGATGAACAAGATCCAACCTTCACAGCTAGCAGAGGAACAAAGAAATGCTTCAAGGCTGTTCAAGAAGCTTCATGAAGAAATCAAAACCACCTTGGCTCTTAAAAACCCAGTTACTTCTTATGATGTGAAAGTCACAATGGAAAAGGTTTTGGCTTTGGATAAGGCATACCCACTTCCCTTAATTGGAACCATGATTGAGAAGTTTCCTCAAATTGTGAAGCCAGCTGTGTGGTGGCCTGAGGAAGAACCCAAGTGCCATGATCAAAACATGAGTGACAAGTTTGAGAGGAATGGGTGGAATGAGAATCTTGAAGAGGAACTGAAGGAGGTTTTGTGCGTAATGAAGGGAAGGGACTCCGCAGAATATGCAAGGCTAAGCAACATGGTGTTGAAGGTCAACAAGGTCTTGGCTTTCTGTGGTCCTTTGCTTACTGGGTCTGCTGCAATTGGGTCAGGTCTTATTGGGTTGCCTGGTTTTGGGTCATGGGGTGCTGTTTTTGGTGTGGTTTTGGGGGCTTTGGGCACTGTGGTTAACAGTTTGGAGCATGGAGGGCAAGTTGGGATGGTGTTTGAGATGTATAGGAGCTCTGCTGGGTTCTTCAAGCTCATGGAGGAGACCATTGAGTCAACTTTGAAGGAAAGAGAGGTTGGGGATAGAGAAAATGGGGAGCTCTTTGAAGTGAAAGTGGCTCTTCAGCTTGGGAGGAGTTTGTCTGAGCTCAGAGACCTTGCAAGTGCTTCATCTTTGTCTTCAAGAATTCAACAGGCCAAAGAAGAATTTGCAAGCAAGCTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

428

Amino Acids

46.92

Weight (kDa)

6.3

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 88 - 404 1.8e-146 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000619)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29861 FvH4_3g26084 FvH4_3g32551 FvH4_4g08193 FvH4_4g12950 FvH4_6g29733
rosa_chinensis RchiOBHm_Chr3g0488921
rosa_multiflora Rmu_co8238041.1_g000001 Rmu_co8265215.1_g000001 Rmu_co8486357.1_g000002 Rmu_sc0000389.1_g000016 Rmu_sc0000580.1_g000123 Rmu_sc0000789.1_g000005 Rmu_sc0001043.1_g000006 Rmu_sc0001208.1_g000001 Rmu_sc0001323.1_g000011 Rmu_sc0001636.1_g000001 Rmu_sc0001911.1_g000023 Rmu_sc0001940.1_g000010 Rmu_sc0002531.1_g000047 Rmu_sc0002715.1_g000007 Rmu_sc0002833.1_g000024 Rmu_sc0003859.1_g000046 Rmu_sc0003894.1_g000008 Rmu_sc0004744.1_g000008 Rmu_sc0004924.1_g000016 Rmu_sc0004991.1_g000004 Rmu_sc0005149.1_g000010 Rmu_sc0005149.1_g000011 Rmu_sc0005489.1_g000007 Rmu_sc0005592.1_g000030 Rmu_sc0005813.1_g000010 Rmu_sc0005813.1_g000011 Rmu_sc0005861.1_g000009 Rmu_sc0005949.1_g000016 Rmu_sc0006824.1_g000021 Rmu_sc0007069.1_g000025 Rmu_sc0008002.1_g000008 Rmu_sc0008148.1_g000008 Rmu_sc0008280.1_g000001 Rmu_sc0008317.1_g000010 Rmu_sc0011615.1_g000002 Rmu_sc0013925.1_g000004 Rmu_sc0014130.1_g000003 Rmu_sc0014510.1_g000008 Rmu_sc0016640.1_g000001 Rmu_sc0018068.1_g000002 Rmu_sc0021371.1_g000006 Rmu_sc0028652.1_g000007 Rmu_sc0032187.1_g000002 Rmu_sc0034241.1_g000001 Rmu_ssc0000127.1_g000003
rosa_roxburghii Rroxscaffold_6G00403950
rosa_rugosa Rorug01G0081700 Rorug02G0544000 Rorug05G0089900 Rorug05G0284500 Rorug05G0452400 Rorug07G0003400 Rorug07G0003400 Rorug07G0129200 Rorug07G0210600
rosa_samantha Rh1AG193900 Rh1AG194000 Rh1DG063400 Rh1DG204700 Rh2AG364300 Rh3BG140200 Rh3CG366000 Rh4DG342600 Rh5AG347700 Rh5BG437300 Rh5CG459900 Rh5DG127400 Rh5DG412800 Rh6BG381500 Rh7CG520500 Rh7DG241900 Rh7DG336000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1111
AciI CCGC 2 cut(s) 392, 846
AclWI GGATC 2 cut(s) 421, 493
AcsI RAATTY 2 cut(s) 1245, 1265
AcuI CTGAAG 3 cut(s) 27, 830, 1166
AfiI CCNNNNNNNGG 3 cut(s) 463, 671, 965
AjnI CCWGG 3 cut(s) 113, 120, 958
AjuI GAANNNNNNNTTGG 2 cut(s) 1119, 1151
Alw21I GWGCWC 4 cut(s) 264, 1076, 1161, 1209
Alw26I GTCTC 5 cut(s) 412, 438, 458, 1097, 1206
AlwI GGATC 2 cut(s) 421, 493
AoxI GGCC 3 cut(s) 3, 722, 1254
ApeKI GCWGC 3 cut(s) 380, 401, 929
ApoI RAATTY 2 cut(s) 1245, 1265
Asp700I GAANNNNTTC 1 cut(s) 535
AspS9I GGNCC 2 cut(s) 419, 908
AsuHPI GGTGA 1 cut(s) 239
AsuNHI GCTAGC 1 cut(s) 514
AvaII GGWCC 2 cut(s) 419, 908
AxyI CCTNAGG 1 cut(s) 726
BaeGI GKGCMC 1 cut(s) 1013
BanII GRGCYC 4 cut(s) 264, 1076, 1161, 1209
BbsI GAAGAC 1 cut(s) 1230
Bbv12I GWGCWC 4 cut(s) 264, 1076, 1161, 1209
BbvI GCAGC 3 cut(s) 367, 413, 916
BccI CCATC 1 cut(s) 1045
BciT130I CCWGG 3 cut(s) 115, 122, 960
BclI TGATCA 1 cut(s) 748
BcoDI GTCTC 5 cut(s) 412, 438, 458, 1097, 1206
BfaI CTAG 3 cut(s) 54, 485, 515
BglII AGATCT 1 cut(s) 441
BisI GCNGC 3 cut(s) 381, 402, 930
BlpI GCTNAGC 1 cut(s) 863
BlsI GCNGC 3 cut(s) 382, 403, 931
Bme1390I CCNGG 3 cut(s) 115, 122, 960
Bme18I GGWCC 2 cut(s) 419, 908
BmgT120I GGNCC 2 cut(s) 419, 908
BmiI GGNNCC 2 cut(s) 119, 676
BmrFI CCNGG 3 cut(s) 115, 122, 960
BmrI ACTGGG 3 cut(s) 483, 590, 930
BmtI GCTAGC 1 cut(s) 518
BmuI ACTGGG 3 cut(s) 483, 590, 930
BpiI GAAGAC 1 cut(s) 1230
Bpu1102I GCTNAGC 1 cut(s) 863
BpuEI CTTGAG 1 cut(s) 422
BsaI GGTCTC 2 cut(s) 1097, 1206
BsaJI CCNNGG 2 cut(s) 113, 579
BsaXI ACNNNNNCTCC 4 cut(s) 806, 836, 1029, 1059
Bsc4I CCNNNNNNNGG 3 cut(s) 463, 671, 965
Bse1I ACTGG 4 cut(s) 338, 478, 596, 925
Bse21I CCTNAGG 1 cut(s) 726
BseBI CCWGG 3 cut(s) 115, 122, 960
BseDI CCNNGG 2 cut(s) 113, 579
BseGI GGATG 3 cut(s) 95, 372, 1056
BseLI CCNNNNNNNGG 3 cut(s) 463, 671, 965
BseMII CTCAG 5 cut(s) 246, 335, 717, 1194, 1222
BseNI ACTGG 4 cut(s) 338, 478, 596, 925
BseRI GAGGAG 2 cut(s) 1115, 1207
BseSI GKGCMC 1 cut(s) 1013
BseXI GCAGC 3 cut(s) 367, 413, 916
BseYI CCCAGC 1 cut(s) 1079
BshFI GGCC 3 cut(s) 5, 724, 1256
BsiHKAI GWGCWC 4 cut(s) 264, 1076, 1161, 1209
BslFI GGGAC 3 cut(s) 341, 408, 854
BslI CCNNNNNNNGG 3 cut(s) 463, 671, 965
BsmAI GTCTC 5 cut(s) 412, 438, 458, 1097, 1206
BsmFI GGGAC 3 cut(s) 341, 408, 854
BsnI GGCC 3 cut(s) 5, 724, 1256
Bso31I GGTCTC 2 cut(s) 1097, 1206
Bsp1286I GDGCHC 5 cut(s) 264, 1013, 1076, 1161, 1209
Bsp143I GATC 5 cut(s) 215, 413, 441, 498, 748
Bsp1720I GCTNAGC 1 cut(s) 863
BspACI CCGC 2 cut(s) 392, 846
BspANI GGCC 3 cut(s) 5, 724, 1256
BspCNI CTCAG 5 cut(s) 245, 334, 718, 1195, 1221
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 2 cut(s) 119, 676
BspOI GCTAGC 1 cut(s) 518
BspPI GGATC 2 cut(s) 421, 493
BspQI GCTCTTC 1 cut(s) 1185
BspTNI GGTCTC 2 cut(s) 1097, 1206
BsrI ACTGG 4 cut(s) 338, 478, 596, 925
BssECI CCNNGG 2 cut(s) 113, 579
BssMI GATC 5 cut(s) 215, 413, 441, 498, 748
BssT1I CCWWGG 1 cut(s) 579
Bst2UI CCWGG 3 cut(s) 115, 122, 960
Bst4CI ACNGT 3 cut(s) 423, 1015, 1025
Bst6I CTCTTC 2 cut(s) 795, 1185
BstAPI GCANNNNNTGC 1 cut(s) 854
BstC8I GCNNGC 4 cut(s) 516, 711, 1273, 1277
BstDEI CTNAG 6 cut(s) 232, 321, 726, 863, 1203, 1208
BstF5I GGATG 3 cut(s) 95, 372, 1056
BstKTI GATC 5 cut(s) 218, 416, 444, 501, 751
BstMAI GTCTC 5 cut(s) 412, 438, 458, 1097, 1206
BstMBI GATC 5 cut(s) 215, 413, 441, 498, 748
BstMWI GCNNNNNNNGC 5 cut(s) 380, 389, 398, 854, 1039
BstNI CCWGG 3 cut(s) 115, 122, 960
BstSCI CCNGG 3 cut(s) 113, 120, 958
BstSLI GKGCMC 1 cut(s) 1013
BstV1I GCAGC 3 cut(s) 367, 413, 916
BstV2I GAAGAC 1 cut(s) 1230
BstX2I RGATCY 3 cut(s) 413, 441, 498
BstXI CCANNNNNNTGG 2 cut(s) 121, 717
BstYI RGATCY 3 cut(s) 413, 441, 498
Bsu36I CCTNAGG 1 cut(s) 726
BsuRI GGCC 3 cut(s) 5, 724, 1256
BtsCI GGATG 3 cut(s) 95, 372, 1056
BtsIMutI CAGTG 1 cut(s) 1011
Cac8I GCNNGC 4 cut(s) 516, 711, 1273, 1277
CciI TCATGA 1 cut(s) 559
Cfr13I GGNCC 2 cut(s) 419, 908
DdeI CTNAG 6 cut(s) 232, 321, 726, 863, 1203, 1208
DpnI GATC 5 cut(s) 217, 415, 443, 500, 750
DpnII GATC 5 cut(s) 215, 413, 441, 498, 748
DrdI GACNNNNNNGTC 1 cut(s) 1111
DseDI GACNNNNNNGTC 1 cut(s) 1111
Eam1104I CTCTTC 2 cut(s) 795, 1185
EarI CTCTTC 2 cut(s) 795, 1185
Ecl136II GAGCTC 4 cut(s) 262, 1074, 1159, 1207
Eco130I CCWWGG 1 cut(s) 579
Eco24I GRGCYC 4 cut(s) 264, 1076, 1161, 1209
Eco31I GGTCTC 2 cut(s) 1097, 1206
Eco47I GGWCC 2 cut(s) 419, 908
Eco53kI GAGCTC 4 cut(s) 262, 1074, 1159, 1207
Eco57I CTGAAG 3 cut(s) 27, 830, 1166
Eco81I CCTNAGG 1 cut(s) 726
EcoICRI GAGCTC 4 cut(s) 262, 1074, 1159, 1207
EcoRI GAATTC 1 cut(s) 1245
EcoRII CCWGG 3 cut(s) 113, 120, 958
EcoT14I CCWWGG 1 cut(s) 579
EcoT38I GRGCYC 4 cut(s) 264, 1076, 1161, 1209
ErhI CCWWGG 1 cut(s) 579
FalI AAGNNNNNCTT 4 cut(s) 520, 552, 1164, 1196
FaqI GGGAC 3 cut(s) 341, 408, 854
FauI CCCGC 1 cut(s) 385
FbaI TGATCA 1 cut(s) 748
Fnu4HI GCNGC 3 cut(s) 381, 402, 930
FokI GGATG 3 cut(s) 82, 379, 1063
FriOI GRGCYC 4 cut(s) 264, 1076, 1161, 1209
Fsp4HI GCNGC 3 cut(s) 381, 402, 930
FspBI CTAG 3 cut(s) 54, 485, 515
GluI GCNGC 3 cut(s) 381, 402, 930
GsaI CCCAGC 1 cut(s) 1083
HaeIII GGCC 3 cut(s) 5, 724, 1256
HincII GTYRAC 3 cut(s) 886, 1021, 1116
HindII GTYRAC 3 cut(s) 886, 1021, 1116
HindIII AAGCTT 3 cut(s) 15, 554, 1277
HinfI GANTC 7 cut(s) 92, 136, 230, 242, 793, 842, 1112
HpaI GTTAAC 1 cut(s) 1021
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 3 cut(s) 886, 1021, 1116
Hpy188I TCNGA 5 cut(s) 178, 241, 324, 1204, 1211
Hpy188III TCNNGA 8 cut(s) 54, 411, 417, 439, 550, 560, 797, 1242
Hpy8I GTNNAC 3 cut(s) 886, 1021, 1116
HpyAV CCTTC 6 cut(s) 516, 805, 826, 831, 874, 1117
HpyCH4III ACNGT 3 cut(s) 423, 1015, 1025
HpyCH4V TGCA 7 cut(s) 304, 383, 401, 857, 932, 1220, 1271
HpyF10VI GCNNNNNNNGC 5 cut(s) 380, 389, 398, 854, 1039
HpyF3I CTNAG 6 cut(s) 232, 321, 726, 863, 1203, 1208
Ksp22I TGATCA 1 cut(s) 748
KspAI GTTAAC 1 cut(s) 1021
Kzo9I GATC 5 cut(s) 215, 413, 441, 498, 748
LguI GCTCTTC 1 cut(s) 1185
LmnI GCTCC 4 cut(s) 123, 1030, 1071, 1156
Lsp1109I GCAGC 3 cut(s) 367, 413, 916
MaeI CTAG 3 cut(s) 54, 485, 515
MaeIII GTNAC 6 cut(s) 150, 227, 469, 598, 619, 761
MalI GATC 5 cut(s) 217, 415, 443, 500, 750
MboI GATC 5 cut(s) 215, 413, 441, 498, 748
MboII GAAGA 8 cut(s) 33, 575, 743, 812, 1078, 1172, 1230, 1274
MfeI CAATTG 1 cut(s) 933
MflI RGATCY 3 cut(s) 413, 441, 498
MhlI GDGCHC 5 cut(s) 264, 1013, 1076, 1161, 1209
MluCI AATT 6 cut(s) 279, 669, 699, 933, 1245, 1265
MlyI GAGTC 3 cut(s) 224, 836, 1121
MmeI TCCRAC 1 cut(s) 526
MroXI GAANNNNTTC 1 cut(s) 535
MseI TTAA 4 cut(s) 126, 588, 668, 1020
MslI CAYNNNNRTG 1 cut(s) 467
MspA1I CMGCKG 3 cut(s) 380, 404, 713
MspR9I CCNGG 3 cut(s) 115, 122, 960
MunI CAATTG 1 cut(s) 933
MvaI CCWGG 3 cut(s) 115, 122, 960
MwoI GCNNNNNNNGC 5 cut(s) 380, 389, 398, 854, 1039
NdeII GATC 5 cut(s) 215, 413, 441, 498, 748
NheI GCTAGC 1 cut(s) 514
NlaIV GGNNCC 2 cut(s) 119, 676
NmuCI GTSAC 5 cut(s) 150, 227, 469, 619, 761
PagI TCATGA 1 cut(s) 559
PciSI GCTCTTC 1 cut(s) 1185
PdmI GAANNNNTTC 1 cut(s) 535
PfeI GAWTC 4 cut(s) 92, 136, 242, 793
PkrI GCNGC 3 cut(s) 382, 403, 931
PleI GAGTC 3 cut(s) 224, 836, 1120
PpsI GAGTC 3 cut(s) 224, 836, 1120
Psp124BI GAGCTC 4 cut(s) 264, 1076, 1161, 1209
Psp6I CCWGG 3 cut(s) 113, 120, 958
PspFI CCCAGC 1 cut(s) 1079
PspGI CCWGG 3 cut(s) 113, 120, 958
PspN4I GGNNCC 2 cut(s) 119, 676
PspPI GGNCC 2 cut(s) 419, 908
PsuI RGATCY 3 cut(s) 413, 441, 498
PvuII CAGCTG 3 cut(s) 380, 404, 713
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 4 cut(s) 264, 1076, 1161, 1209
SapI GCTCTTC 1 cut(s) 1185
SaqAI TTAA 4 cut(s) 126, 588, 668, 1020
SatI GCNGC 3 cut(s) 381, 402, 930
Sau3AI GATC 5 cut(s) 215, 413, 441, 498, 748
Sau96I GGNCC 2 cut(s) 419, 908
SchI GAGTC 3 cut(s) 224, 836, 1121
ScrFI CCNGG 3 cut(s) 115, 122, 960
SduI GDGCHC 5 cut(s) 264, 1013, 1076, 1161, 1209
SinI GGWCC 2 cut(s) 419, 908
SmiMI CAYNNNNRTG 1 cut(s) 467
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
Sse9I AATT 6 cut(s) 279, 669, 699, 933, 1245, 1265
SsiI CCGC 2 cut(s) 392, 846
SspMI CTAG 3 cut(s) 54, 485, 515
SstI GAGCTC 4 cut(s) 264, 1076, 1161, 1209
StyD4I CCNGG 3 cut(s) 113, 120, 958
StyI CCWWGG 1 cut(s) 579
TaaI ACNGT 3 cut(s) 423, 1015, 1025
TasI AATT 6 cut(s) 279, 669, 699, 933, 1245, 1265
TfiI GAWTC 4 cut(s) 92, 136, 242, 793
Tru1I TTAA 4 cut(s) 126, 588, 668, 1020
Tru9I TTAA 4 cut(s) 126, 588, 668, 1020
TscAI CASTG 1 cut(s) 1018
TseFI GTSAC 5 cut(s) 150, 227, 469, 619, 761
TseI GCWGC 3 cut(s) 380, 401, 929
Tsp45I GTSAC 5 cut(s) 150, 227, 469, 619, 761
TspDTI ATGAA 8 cut(s) 24, 144, 162, 506, 548, 576, 845, 1218
TspRI CASTG 1 cut(s) 1018
VpaK11BI GGWCC 2 cut(s) 419, 908
XapI RAATTY 2 cut(s) 1245, 1265
XbaI TCTAGA 1 cut(s) 53
XcmI CCANNNNNNNNNTGG 1 cut(s) 717
XmnI GAANNNNTTC 1 cut(s) 535
XspI CTAG 3 cut(s) 54, 485, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.