Rorug01G0081700

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000619
Physical Location & Seq
Reverse (-)
13253015 .. 13255756
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0081700.1

Sequence Viewer

Length: 2289 bp
ATGGGCGAGCCTCCTCCAGCCTGCCCGTGGAGTTTTCTTCAGCCATTCTCTGACACGAGATCTCCCTCTTCTACATCTGAACAGAAAATTCACAAGGCAAAGACCTTTGCATCCATTGTTGCAGGTTCAGAGGAAACCCAAGTTCCTATCAGCCAACTACCAGCGCCAACAATTCGTGGAGACACGGTTTATGTGAAGATCAATGAGAGGATCTATCAAGAACAATTAACAGCCTGCCGAAATAATTTGATCGGTCGATTGCTACTCCGTAAGGGCACTATTCCATTGAAAACTGAAACTCTCAAGGCTTCTCTGCATACTCTTTGGCGTCCGTTGGGTCCGTGGCGTCTGGTCCCCATTGGGAAAGGGTATTTTGACATTCACTTCAACTCTGAAGCAGATATGCGCAAAGTTTGGGGCGGTGGGACTTGCACTCTCGAATCTGGAATTTTTCGTCTGTCTCAATGGAAACCTGATTTCAGGCCTGGTGATATGCTTCCCCAAACGCATGCACAAATCTGGGTACGTATTTCAGGCCTTAGTCAAGAGTATTGGCACCAACAACACATTCTTGAAATTGCTCGGGGAGTGGGCACCCCGTTGCAATTGGATGCGGCAACTAAGGAACAACGATATGGATATTATGCTAGAGTGTTGGTGGATGTTGATCTGTTGGGTGCTCTTCCTACGTCCATTATGGTTGAAAGAGAACACCTTTGTTTTCCAGTAGGTATTGAGTATGAAAACCTTCCTCCTCAGTGTTCTCATTGTGGTTTGATAGGGCACGAAAGGAATAATTGCAGGCAACTAAAGTCCAAGGAAGTTCCTGCGAAACGTAAAGAGGTTCGTCAAGAATATCGTATAAAGAATAAATTGCAGGAAACCCATGTTCAAGAGGCTAGCTTCTCTGACGCACATGCCATTAACGATATTGACGGTACTGTTGTTACTTTGAATGCCATAGAGCAACCTTTGGCAGGTGATCAAGCTCCCATCCAAAGTGATGTTGTTGTGGCTCTTGTAAATACTGTTTTGGAGGAAGCAGCGGCAGAGGTTATGGAGGAACAAGCTCAAAGGGTAATGGAGGAGAATAATTCTAACCTAGCCTCTAATGCTGCATCTGATGCTTCGGACACAGATATTAGTGATTCACCTACCCGTACTTGGGGGGATATTTTTGAGGAGGAGAGGGTTGAAGGTTTGATCAGAGAGGCTCCTTCTAGTGCTCCACCACGCCTTGGTCCACCTCCTGGTTTTGTCAAATCTAATGTGGCTTCAGAAATTGATGCGGTTGCAAGATTTGTTAATGAGCCAATGATTCCTTTTGACTCTGTTCCTACTGCTTACTGGGCTTCCTTGAATCTACGACTAATTGCGGTAAATGATAGAGGTACTCTTCTTCCAAATATCTGGCTTCTTGGTTCTTTACATATCTCTTCTCCTACACTAGTGCTCTCTGCAGATCAACACATTACTATTCAGATTACTTTGGAAGGCACTCTTACTCAACTATCTTGTGTCTACGCGGCTACAACTGCACTAAAACGTCGCAAATTATGGTCGGATTTATTACTTCTTCGTCAACAGACCACCATTCCATGGATGGCAATAGGAGACTTTAATGCAGTATTGGGGGCTCATGAGGCCATGAATGGGAATATCCCTACGCGTTCATCTTGTGAGGATTTTCGCGATGCCACAGAACGATGTCATTTCACCCATCTTGATACTTTAGGGGCTTTCTTTACATGGTCTAAAGGGCGCGGTCGGAAGCACTTGGAACGACGGCTTGATCGCTCCTTATGTGATGATGGCTGGTTTGAGGCTTGGCCATTTACCAACTGTATAGCTCTTCCCCGAGTGGTTTCGGATCATAATGGTTTACTTTTTTCAGCCGGTAAGTCTGTACCAGGGGGTCCTAAACCTTTTCGTTTCCAATCTATGTGGCTTCTCCACTCTTCTTTCAAGGAGTTGGTGGCGAATTGTTGGTCGACCACCAGGCCAGCTGGTTGTCCCATAAACTTGGAGTTGGCCCGTGCTAAGTTGACTGCTATTCAACAAGATATTGCTTCTAATGGTTCTGCTGATATTAACTTTGAGGCTGAAGTTCTTGCAAAGGCCGATGTTCTTAATGCGGTTAAGCATCAGGAGACTTTTTGGAGGGACAGAGCTCGTGTTAAATGGTTTACCGAGGGGGATAAATGCTCAAATTTTTTCCATGCTTATGCCCGCAATAAGGCAAGGCGCTCTTCAATGCATTGCTTACAGATGTTGCTCCAAGTGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

762

Amino Acids

85.24

Weight (kDa)

6.53

Isoelectric Point (pI)

47.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 79 - 162 9.8e-07 Domain of unknown function (DUF4283)
Exo_endo_phos PF03372 506 - 625 5.8e-06 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000619)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29861 FvH4_3g26084 FvH4_3g32551 FvH4_4g08193 FvH4_4g12950 FvH4_6g29733
rosa_chinensis RchiOBHm_Chr3g0488921
rosa_multiflora Rmu_co8238041.1_g000001 Rmu_co8265215.1_g000001 Rmu_co8486357.1_g000002 Rmu_sc0000389.1_g000016 Rmu_sc0000580.1_g000123 Rmu_sc0000789.1_g000005 Rmu_sc0001043.1_g000006 Rmu_sc0001208.1_g000001 Rmu_sc0001323.1_g000011 Rmu_sc0001636.1_g000001 Rmu_sc0001911.1_g000023 Rmu_sc0001940.1_g000010 Rmu_sc0002531.1_g000047 Rmu_sc0002715.1_g000007 Rmu_sc0002833.1_g000024 Rmu_sc0003859.1_g000046 Rmu_sc0003894.1_g000008 Rmu_sc0004744.1_g000008 Rmu_sc0004924.1_g000016 Rmu_sc0004991.1_g000004 Rmu_sc0005149.1_g000010 Rmu_sc0005149.1_g000011 Rmu_sc0005489.1_g000007 Rmu_sc0005592.1_g000030 Rmu_sc0005813.1_g000010 Rmu_sc0005813.1_g000011 Rmu_sc0005861.1_g000009 Rmu_sc0005949.1_g000016 Rmu_sc0006824.1_g000021 Rmu_sc0007069.1_g000025 Rmu_sc0008002.1_g000008 Rmu_sc0008148.1_g000008 Rmu_sc0008280.1_g000001 Rmu_sc0008317.1_g000010 Rmu_sc0011615.1_g000002 Rmu_sc0013925.1_g000004 Rmu_sc0014130.1_g000003 Rmu_sc0014510.1_g000008 Rmu_sc0016640.1_g000001 Rmu_sc0018068.1_g000002 Rmu_sc0021371.1_g000006 Rmu_sc0028652.1_g000007 Rmu_sc0032187.1_g000002 Rmu_sc0034241.1_g000001 Rmu_ssc0000127.1_g000003
rosa_roxburghii Rroxscaffold_6G00403950
rosa_rugosa Rorug01G0081700 Rorug02G0544000 Rorug05G0089900 Rorug05G0284500 Rorug05G0452400 Rorug07G0003400 Rorug07G0003400 Rorug07G0129200 Rorug07G0210600
rosa_samantha Rh1AG193900 Rh1AG194000 Rh1DG063400 Rh1DG204700 Rh2AG364300 Rh3BG140200 Rh3CG366000 Rh4DG342600 Rh5AG347700 Rh5BG437300 Rh5CG459900 Rh5DG127400 Rh5DG412800 Rh6BG381500 Rh7CG520500 Rh7DG241900 Rh7DG336000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 968
Acc16I TGCGCA 1 cut(s) 407
Acc36I ACCTGC 2 cut(s) 113, 968
AccB1I GGYRCC 2 cut(s) 555, 593
AccB7I CCANNNNNTGG 3 cut(s) 1238, 1250, 1599
AccI GTMKAC 2 cut(s) 1521, 1991
AccII CGCG 4 cut(s) 1526, 1669, 1692, 1764
AciI CCGC 9 cut(s) 420, 614, 1046, 1289, 1376, 1526, 1764, 2135, 2230
AclWI GGATC 2 cut(s) 218, 1878
AcoI YGGCCR 1 cut(s) 1829
AcsI RAATTY 3 cut(s) 87, 447, 2209
AcuI CTGAAG 4 cut(s) 23, 414, 1260, 2124
AcyI GRCGYC 2 cut(s) 328, 346
AfaI GTAC 5 cut(s) 525, 940, 1162, 1393, 1908
AfiI CCNNNNNNNGG 9 cut(s) 27, 977, 1164, 1165, 1238, 1250, 1599, 1653, 2236
AflIII ACRYGT 1 cut(s) 1667
AhlI ACTAGT 1 cut(s) 1447
AjnI CCWGG 4 cut(s) 484, 1249, 1909, 1997
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AluBI AGCT 6 cut(s) 903, 989, 1070, 1850, 2006, 2171
AluI AGCT 6 cut(s) 903, 989, 1070, 1850, 2006, 2171
Alw21I GWGCWC 4 cut(s) 682, 1228, 1455, 2173
Alw26I GTCTC 4 cut(s) 174, 465, 1608, 2144
AlwI GGATC 2 cut(s) 218, 1878
Ama87I CYCGRG 2 cut(s) 582, 1857
AoxI GGCC 7 cut(s) 482, 535, 1644, 1829, 2000, 2031, 2118
ApeKI GCWGC 2 cut(s) 1043, 1115
ApoI RAATTY 3 cut(s) 87, 447, 2209
ArsI GACNNNNNNTTYG 2 cut(s) 1973, 2005
Asp700I GAANNNNTTC 1 cut(s) 747
AspLEI GCGC 4 cut(s) 166, 408, 1764, 2247
AspS9I GGNCC 5 cut(s) 338, 352, 1241, 1916, 2032
AsuHPI GGTGA 4 cut(s) 500, 992, 1143, 1708
AsuNHI GCTAGC 1 cut(s) 899
AvaI CYCGRG 2 cut(s) 582, 1857
AvaII GGWCC 4 cut(s) 338, 352, 1241, 1916
BaeGI GKGCMC 3 cut(s) 278, 596, 786
BaeI ACNNNNGTAYC 2 cut(s) 930, 963
BalI TGGCCA 1 cut(s) 1831
BanI GGYRCC 2 cut(s) 555, 593
BanII GRGCYC 2 cut(s) 1639, 2173
BauI CACGAG 2 cut(s) 55, 2172
Bbv12I GWGCWC 4 cut(s) 682, 1228, 1455, 2173
BbvI GCAGC 2 cut(s) 1055, 1102
BccI CCATC 4 cut(s) 1001, 1597, 1728, 1805
BceAI ACGGC 1 cut(s) 1802
BciT130I CCWGG 4 cut(s) 486, 1251, 1911, 1999
BclI TGATCA 2 cut(s) 982, 1203
BcoDI GTCTC 4 cut(s) 174, 465, 1608, 2144
BcuI ACTAGT 1 cut(s) 1447
BfaI CTAG 5 cut(s) 648, 900, 1103, 1221, 1448
BfmI CTRYAG 1 cut(s) 1458
BfoI RGCGCY 2 cut(s) 167, 2248
BfuAI ACCTGC 2 cut(s) 113, 968
BglII AGATCT 1 cut(s) 59
BisI GCNGC 5 cut(s) 615, 1044, 1047, 1116, 1527
BlsI GCNGC 5 cut(s) 616, 1045, 1048, 1117, 1528
Bme1390I CCNGG 4 cut(s) 486, 1251, 1911, 1999
Bme18I GGWCC 4 cut(s) 338, 352, 1241, 1916
BmeT110I CYCGRG 2 cut(s) 582, 1857
BmgT120I GGNCC 5 cut(s) 338, 352, 1241, 1916, 2032
BmiI GGNNCC 6 cut(s) 339, 354, 557, 595, 1215, 1917
BmrFI CCNGG 4 cut(s) 486, 1251, 1911, 1999
BmrI ACTGGG 1 cut(s) 1357
BmsI GCATC 7 cut(s) 119, 601, 1114, 1127, 1276, 1684, 2152
BmtI GCTAGC 1 cut(s) 903
BmuI ACTGGG 1 cut(s) 1357
BpuEI CTTGAG 1 cut(s) 287
BsaAI YACGTR 1 cut(s) 527
BsaBI GATNNNNATC 1 cut(s) 666
BsaHI GRCGYC 2 cut(s) 328, 346
BsaJI CCNNGG 7 cut(s) 26, 341, 816, 1237, 1598, 1910, 2190
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 171, 201
Bsc4I CCNNNNNNNGG 9 cut(s) 27, 977, 1164, 1165, 1238, 1250, 1599, 1653, 2236
Bse118I RCCGGY 1 cut(s) 1895
Bse1I ACTGG 2 cut(s) 725, 1352
Bse3DI GCAATG 1 cut(s) 2257
Bse8I GATNNNNATC 1 cut(s) 666
BseBI CCWGG 4 cut(s) 486, 1251, 1911, 1999
BseDI CCNNGG 7 cut(s) 26, 341, 816, 1237, 1598, 1910, 2190
BseGI GGATG 5 cut(s) 110, 616, 667, 993, 1608
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 9 cut(s) 27, 977, 1164, 1165, 1238, 1250, 1599, 1653, 2236
BseMI GCAATG 1 cut(s) 2257
BseMII CTCAG 1 cut(s) 770
BseNI ACTGG 2 cut(s) 725, 1352
BseRI GAGGAG 4 cut(s) 744, 1100, 1196, 1199
BseSI GKGCMC 3 cut(s) 278, 596, 786
BseXI GCAGC 2 cut(s) 1055, 1102
BsgI GTGCAG 1 cut(s) 1521
Bsh1236I CGCG 4 cut(s) 1526, 1669, 1692, 1764
Bsh1285I CGRYCG 2 cut(s) 256, 1768
BshFI GGCC 7 cut(s) 484, 537, 1646, 1831, 2002, 2033, 2120
BshNI GGYRCC 2 cut(s) 555, 593
BsiEI CGRYCG 2 cut(s) 256, 1768
BsiHKAI GWGCWC 4 cut(s) 682, 1228, 1455, 2173
BsiHKCI CYCGRG 2 cut(s) 582, 1857
BsiSI CCGG 1 cut(s) 1896
BslFI GGGAC 4 cut(s) 338, 439, 1998, 2177
BslI CCNNNNNNNGG 9 cut(s) 27, 977, 1164, 1165, 1238, 1250, 1599, 1653, 2236
BsmAI GTCTC 4 cut(s) 174, 465, 1608, 2144
BsmFI GGGAC 4 cut(s) 338, 439, 1998, 2177
BsmI GAATGC 1 cut(s) 961
BsnI GGCC 7 cut(s) 484, 537, 1646, 1831, 2002, 2033, 2120
BsoBI CYCGRG 2 cut(s) 582, 1857
Bsp1286I GDGCHC 8 cut(s) 278, 596, 682, 786, 1228, 1455, 1639, 2173
Bsp19I CCATGG 1 cut(s) 1598
Bsp68I TCGCGA 1 cut(s) 1692
BspACI CCGC 9 cut(s) 420, 614, 1046, 1289, 1376, 1526, 1764, 2135, 2230
BspANI GGCC 7 cut(s) 484, 537, 1646, 1831, 2002, 2033, 2120
BspCNI CTCAG 1 cut(s) 769
BspFNI CGCG 4 cut(s) 1526, 1669, 1692, 1764
BspHI TCATGA 1 cut(s) 1639
BspLI GGNNCC 6 cut(s) 339, 354, 557, 595, 1215, 1917
BspMAI CTGCAG 1 cut(s) 1462
BspMI ACCTGC 2 cut(s) 113, 968
BspOI GCTAGC 1 cut(s) 903
BspPI GGATC 2 cut(s) 218, 1878
BspQI GCTCTTC 3 cut(s) 687, 1857, 2254
BspT107I GGYRCC 2 cut(s) 555, 593
BsrDI GCAATG 1 cut(s) 2257
BsrFI RCCGGY 1 cut(s) 1895
BsrI ACTGG 2 cut(s) 725, 1352
BssAI RCCGGY 1 cut(s) 1895
BssECI CCNNGG 7 cut(s) 26, 341, 816, 1237, 1598, 1910, 2190
BssNI GRCGYC 2 cut(s) 328, 346
BssSI CACGAG 2 cut(s) 55, 2172
BssT1I CCWWGG 3 cut(s) 816, 1237, 1598
Bst2BI CACGAG 2 cut(s) 55, 2172
Bst2UI CCWGG 4 cut(s) 486, 1251, 1911, 1999
Bst4CI ACNGT 5 cut(s) 187, 938, 943, 1030, 1844
Bst6I CTCTTC 7 cut(s) 73, 687, 1401, 1441, 1857, 1963, 2254
BstACI GRCGYC 2 cut(s) 328, 346
BstAPI GCANNNNNTGC 1 cut(s) 1124
BstBAI YACGTR 1 cut(s) 527
BstC8I GCNNGC 8 cut(s) 8, 22, 235, 510, 803, 901, 2004, 2230
BstDEI CTNAG 4 cut(s) 539, 621, 756, 2040
BstDSI CCRYGG 3 cut(s) 26, 341, 1598
BstENI CCTNNNNNAGG 1 cut(s) 975
BstF5I GGATG 5 cut(s) 110, 616, 667, 993, 1608
BstFNI CGCG 4 cut(s) 1526, 1669, 1692, 1764
BstH2I RGCGCY 2 cut(s) 167, 2248
BstHHI GCGC 4 cut(s) 166, 408, 1764, 2247
BstMAI GTCTC 4 cut(s) 174, 465, 1608, 2144
BstMCI CGRYCG 2 cut(s) 256, 1768
BstMWI GCNNNNNNNGC 5 cut(s) 1112, 1124, 1349, 1535, 1643
BstNI CCWGG 4 cut(s) 486, 1251, 1911, 1999
BstNSI RCATGY 2 cut(s) 512, 920
BstSCI CCNGG 4 cut(s) 484, 1249, 1909, 1997
BstSFI CTRYAG 1 cut(s) 1458
BstSLI GKGCMC 3 cut(s) 278, 596, 786
BstSNI TACGTA 1 cut(s) 527
BstUI CGCG 4 cut(s) 1526, 1669, 1692, 1764
BstV1I GCAGC 2 cut(s) 1055, 1102
BstX2I RGATCY 2 cut(s) 59, 210
BstXI CCANNNNNNTGG 2 cut(s) 1410, 2023
BstYI RGATCY 2 cut(s) 59, 210
BsuRI GGCC 7 cut(s) 484, 537, 1646, 1831, 2002, 2033, 2120
BtgI CCRYGG 3 cut(s) 26, 341, 1598
BtgZI GCGATG 1 cut(s) 1707
BtsCI GGATG 5 cut(s) 110, 616, 667, 993, 1608
BtsIMutI CAGTG 1 cut(s) 764
BtuMI TCGCGA 1 cut(s) 1692
BveI ACCTGC 2 cut(s) 113, 968
Cac8I GCNNGC 8 cut(s) 8, 22, 235, 510, 803, 901, 2004, 2230
CciI TCATGA 1 cut(s) 1639
CfoI GCGC 4 cut(s) 166, 408, 1764, 2247
Cfr10I RCCGGY 1 cut(s) 1895
Cfr13I GGNCC 5 cut(s) 338, 352, 1241, 1916, 2032
CseI GACGC 3 cut(s) 317, 335, 920
Csp6I GTAC 5 cut(s) 524, 939, 1161, 1392, 1907
CspCI CAANNNNNGTGG 2 cut(s) 1925, 1960
CviAII CATG 8 cut(s) 509, 887, 917, 1599, 1640, 1648, 1749, 2219
CviQI GTAC 5 cut(s) 524, 939, 1161, 1392, 1907
DdeI CTNAG 4 cut(s) 539, 621, 756, 2040
EaeI YGGCCR 1 cut(s) 1829
Eam1104I CTCTTC 7 cut(s) 73, 687, 1401, 1441, 1857, 1963, 2254
EarI CTCTTC 7 cut(s) 73, 687, 1401, 1441, 1857, 1963, 2254
Ecl136II GAGCTC 1 cut(s) 2171
Eco105I TACGTA 1 cut(s) 527
Eco130I CCWWGG 3 cut(s) 816, 1237, 1598
Eco147I AGGCCT 2 cut(s) 484, 537
Eco24I GRGCYC 2 cut(s) 1639, 2173
Eco47I GGWCC 4 cut(s) 338, 352, 1241, 1916
Eco53kI GAGCTC 1 cut(s) 2171
Eco57I CTGAAG 4 cut(s) 23, 414, 1260, 2124
Eco88I CYCGRG 2 cut(s) 582, 1857
EcoICRI GAGCTC 1 cut(s) 2171
EcoNI CCTNNNNNAGG 1 cut(s) 975
EcoO109I RGGNCCY 1 cut(s) 1916
EcoRII CCWGG 4 cut(s) 484, 1249, 1909, 1997
EcoT14I CCWWGG 3 cut(s) 816, 1237, 1598
EcoT22I ATGCAT 1 cut(s) 2259
EcoT38I GRGCYC 2 cut(s) 1639, 2173
ErhI CCWWGG 3 cut(s) 816, 1237, 1598
FaeI CATG 8 cut(s) 512, 890, 920, 1602, 1643, 1651, 1752, 2222
FalI AAGNNNNNCTT 4 cut(s) 1485, 1517, 2233, 2265
FaqI GGGAC 4 cut(s) 338, 439, 1998, 2177
FatI CATG 8 cut(s) 508, 886, 916, 1598, 1639, 1647, 1748, 2218
FauI CCCGC 1 cut(s) 2237
FbaI TGATCA 2 cut(s) 982, 1203
FblI GTMKAC 2 cut(s) 1521, 1991
Fnu4HI GCNGC 5 cut(s) 615, 1044, 1047, 1116, 1527
FokI GGATG 5 cut(s) 97, 623, 674, 980, 1615
FriOI GRGCYC 2 cut(s) 1639, 2173
Fsp4HI GCNGC 5 cut(s) 615, 1044, 1047, 1116, 1527
FspBI CTAG 5 cut(s) 648, 900, 1103, 1221, 1448
FspI TGCGCA 1 cut(s) 407
GlaI GCGC 4 cut(s) 165, 407, 1763, 2246
GluI GCNGC 5 cut(s) 615, 1044, 1047, 1116, 1527
HaeII RGCGCY 2 cut(s) 167, 2248
HaeIII GGCC 7 cut(s) 484, 537, 1646, 1831, 2002, 2033, 2120
HapII CCGG 1 cut(s) 1896
HgaI GACGC 3 cut(s) 317, 335, 920
HhaI GCGC 4 cut(s) 166, 408, 1764, 2247
Hin1I GRCGYC 2 cut(s) 328, 346
Hin1II CATG 8 cut(s) 512, 890, 920, 1602, 1643, 1651, 1752, 2222
Hin6I GCGC 4 cut(s) 164, 406, 1762, 2245
HinP1I GCGC 4 cut(s) 164, 406, 1762, 2245
HincII GTYRAC 3 cut(s) 1583, 1992, 2046
HindII GTYRAC 3 cut(s) 1583, 1992, 2046
HinfI GANTC 5 cut(s) 440, 1148, 1318, 1328, 1360
HpaII CCGG 1 cut(s) 1896
HphI GGTGA 4 cut(s) 500, 992, 1143, 1708
Hpy166II GTNNAC 7 cut(s) 1244, 1522, 1583, 1883, 1992, 2046, 2187
Hpy8I GTNNAC 7 cut(s) 1244, 1522, 1583, 1883, 1992, 2046, 2187
Hpy99I CGWCG 2 cut(s) 1551, 1788
HpyAV CCTTC 4 cut(s) 758, 1190, 1227, 1487
HpyCH4III ACNGT 5 cut(s) 187, 938, 943, 1030, 1844
HpyCH4IV ACGT 4 cut(s) 526, 689, 835, 1546
HpyF10VI GCNNNNNNNGC 5 cut(s) 1112, 1124, 1349, 1535, 1643
HpyF3I CTNAG 4 cut(s) 539, 621, 756, 2040
HpySE526I ACGT 4 cut(s) 526, 689, 835, 1546
Hsp92I GRCGYC 2 cut(s) 328, 346
Hsp92II CATG 8 cut(s) 512, 890, 920, 1602, 1643, 1651, 1752, 2222
HspAI GCGC 4 cut(s) 164, 406, 1762, 2245
Ksp22I TGATCA 2 cut(s) 982, 1203
LguI GCTCTTC 3 cut(s) 687, 1857, 2254
LmnI GCTCC 5 cut(s) 994, 1219, 1231, 1802, 2280
Lsp1109I GCAGC 2 cut(s) 1055, 1102
LweI GCATC 7 cut(s) 119, 601, 1114, 1127, 1276, 1684, 2152
MaeI CTAG 5 cut(s) 648, 900, 1103, 1221, 1448
MaeII ACGT 4 cut(s) 526, 689, 835, 1546
MaeIII GTNAC 1 cut(s) 946
MfeI CAATTG 1 cut(s) 605
MflI RGATCY 2 cut(s) 59, 210
MhlI GDGCHC 8 cut(s) 278, 596, 682, 786, 1228, 1455, 1639, 2173
MlsI TGGCCA 1 cut(s) 1831
MluI ACGCGT 1 cut(s) 1667
MluNI TGGCCA 1 cut(s) 1831
MlyI GAGTC 1 cut(s) 1322
MmeI TCCRAC 2 cut(s) 1542, 1748
Mox20I TGGCCA 1 cut(s) 1831
Mph1103I ATGCAT 1 cut(s) 2259
MroXI GAANNNNTTC 1 cut(s) 747
MscI TGGCCA 1 cut(s) 1831
MseI TTAA 8 cut(s) 227, 924, 1305, 1620, 2091, 2130, 2139, 2178
MslI CAYNNNNRTG 1 cut(s) 2223
Msp20I TGGCCA 1 cut(s) 1831
MspA1I CMGCKG 2 cut(s) 1046, 2006
MspI CCGG 1 cut(s) 1896
MspR9I CCNGG 4 cut(s) 486, 1251, 1911, 1999
MunI CAATTG 1 cut(s) 605
Mva1269I GAATGC 1 cut(s) 961
MvaI CCWGG 4 cut(s) 486, 1251, 1911, 1999
MvnI CGCG 4 cut(s) 1526, 1669, 1692, 1764
MwoI GCNNNNNNNGC 5 cut(s) 1112, 1124, 1349, 1535, 1643
NcoI CCATGG 1 cut(s) 1598
NheI GCTAGC 1 cut(s) 899
NlaIII CATG 8 cut(s) 512, 890, 920, 1602, 1643, 1651, 1752, 2222
NlaIV GGNNCC 6 cut(s) 339, 354, 557, 595, 1215, 1917
NruI TCGCGA 1 cut(s) 1692
NsbI TGCGCA 1 cut(s) 407
NsiI ATGCAT 1 cut(s) 2259
NspI RCATGY 2 cut(s) 512, 920
PaeI GCATGC 1 cut(s) 512
PagI TCATGA 1 cut(s) 1639
PaqCI CACCTGC 1 cut(s) 968
PceI AGGCCT 2 cut(s) 484, 537
PciSI GCTCTTC 3 cut(s) 687, 1857, 2254
PctI GAATGC 1 cut(s) 961
PdmI GAANNNNTTC 1 cut(s) 747
PfeI GAWTC 4 cut(s) 440, 1148, 1318, 1360
PflMI CCANNNNNTGG 3 cut(s) 1238, 1250, 1599
PkrI GCNGC 5 cut(s) 616, 1045, 1048, 1117, 1528
PleI GAGTC 1 cut(s) 1322
PpsI GAGTC 1 cut(s) 1322
Ppu21I YACGTR 1 cut(s) 527
PpuMI RGGWCCY 1 cut(s) 1916
Psp124BI GAGCTC 1 cut(s) 2173
Psp5II RGGWCCY 1 cut(s) 1916
Psp6I CCWGG 4 cut(s) 484, 1249, 1909, 1997
PspGI CCWGG 4 cut(s) 484, 1249, 1909, 1997
PspN4I GGNNCC 6 cut(s) 339, 354, 557, 595, 1215, 1917
PspPI GGNCC 5 cut(s) 338, 352, 1241, 1916, 2032
PspPPI RGGWCCY 1 cut(s) 1916
PstI CTGCAG 1 cut(s) 1462
PsuI RGATCY 2 cut(s) 59, 210
PvuII CAGCTG 1 cut(s) 2006
RruI TCGCGA 1 cut(s) 1692
RsaI GTAC 5 cut(s) 525, 940, 1162, 1393, 1908
RsaNI GTAC 5 cut(s) 524, 939, 1161, 1392, 1907
RseI CAYNNNNRTG 1 cut(s) 2223
SacI GAGCTC 1 cut(s) 2173
SalI GTCGAC 1 cut(s) 1990
SapI GCTCTTC 3 cut(s) 687, 1857, 2254
SaqAI TTAA 8 cut(s) 227, 924, 1305, 1620, 2091, 2130, 2139, 2178
SatI GCNGC 5 cut(s) 615, 1044, 1047, 1116, 1527
Sau96I GGNCC 5 cut(s) 338, 352, 1241, 1916, 2032
SchI GAGTC 1 cut(s) 1322
ScrFI CCNGG 4 cut(s) 486, 1251, 1911, 1999
SduI GDGCHC 8 cut(s) 278, 596, 682, 786, 1228, 1455, 1639, 2173
SfaNI GCATC 7 cut(s) 119, 601, 1114, 1127, 1276, 1684, 2152
SfcI CTRYAG 1 cut(s) 1458
SinI GGWCC 4 cut(s) 338, 352, 1241, 1916
SmiMI CAYNNNNRTG 1 cut(s) 2223
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SnaBI TACGTA 1 cut(s) 527
SpeI ACTAGT 1 cut(s) 1447
SphI GCATGC 1 cut(s) 512
SseBI AGGCCT 2 cut(s) 484, 537
SsiI CCGC 9 cut(s) 420, 614, 1046, 1289, 1376, 1526, 1764, 2135, 2230
SspMI CTAG 5 cut(s) 648, 900, 1103, 1221, 1448
SstI GAGCTC 1 cut(s) 2173
StuI AGGCCT 2 cut(s) 484, 537
StyD4I CCNGG 4 cut(s) 484, 1249, 1909, 1997
StyI CCWWGG 3 cut(s) 816, 1237, 1598
TaaI ACNGT 5 cut(s) 187, 938, 943, 1030, 1844
TaiI ACGT 4 cut(s) 529, 692, 838, 1549
TaqI TCGA 3 cut(s) 256, 438, 1991
TaqII GACCGA 1 cut(s) 242
TauI GCSGC 3 cut(s) 617, 1049, 1529
TfiI GAWTC 4 cut(s) 440, 1148, 1318, 1360
Tru1I TTAA 8 cut(s) 227, 924, 1305, 1620, 2091, 2130, 2139, 2178
Tru9I TTAA 8 cut(s) 227, 924, 1305, 1620, 2091, 2130, 2139, 2178
TscAI CASTG 1 cut(s) 764
TseI GCWGC 2 cut(s) 1043, 1115
TspDTI ATGAA 3 cut(s) 756, 1662, 1664
TspGWI ACGGA 3 cut(s) 257, 321, 330
TspRI CASTG 1 cut(s) 764
Van91I CCANNNNNTGG 3 cut(s) 1238, 1250, 1599
VpaK11BI GGWCC 4 cut(s) 338, 352, 1241, 1916
XagI CCTNNNNNAGG 1 cut(s) 975
XapI RAATTY 3 cut(s) 87, 447, 2209
XceI RCATGY 2 cut(s) 512, 920
XcmI CCANNNNNNNNNTGG 2 cut(s) 24, 1600
XmiI GTMKAC 2 cut(s) 1521, 1991
XmnI GAANNNNTTC 1 cut(s) 747
XspI CTAG 5 cut(s) 648, 900, 1103, 1221, 1448
Zsp2I ATGCAT 1 cut(s) 2259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.