Rmu_sc0005813.1_g000010

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005813.1
OG0000619
Physical Location & Seq
Forward (+)
26337 .. 30630
4294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005813.1_g000010.1.cds

Sequence Viewer

Length: 2790 bp
atggaagaggagttagagcagttagtcagaaaactgactctgaaggaggaaaaagcccaggatgtgttcctggcagacacggatgggaaatggaagcctcgaaaatttctgttggtaggtcggttgctaaccaagaaaacctaccgaatggaggcgctgattgggaccatgagagccctgtggtccccaaggaaaaataggggtgagaatccacgcttgacaacgtgtgctctagaggggagcgatagggtggtgttctcctttacctatgagtatgatcgaaataaagtgttgtggggatgcccttggcatttcgataaggcgctttttgcagctgcagcgactgatgggactgaggatctgctacatgtgtcattggatttgcaatttttttggatcagggcctggggagttccatcgctgtatatggaggaggaaacgggtatcagaattgggaatgcggtgggaaggtttgctagaatgggtgaggccactaatgggttattcttgggttcctctttgaaggtgcaattgatgatagatgtctctacaccgctcataaggggtggcctcggtcaggttacccagacaagagcgagatgggtggatggcagagaggatggagtcggagaaggagggtcagtcagcatttggtggactggatgtcgttatggtctcattacgaaagagaagggaggttggtgcatgcaggttccggacttccctatttctggtatggttcgatcgagagctgaatggaaagatgatgatagccaggcgcggtcgggggcggaggtggacggtagcgagctacgggacattcccagcaatgagagcccggaactttcacaggcattgacggggattggagctattaatggaaagaggttacagaagattgacataccaaagtttgggcggggaacaggggcggatttgcagggtttggattttaattccctaaatgggctggttgatgggtcttgggccagggaaaaaaaaggaagtggcatggttgggttggatgggccaaagggggctgttagagattctggtagtggagagaagttgtttgagccggatacaacaacttttttgcttggggtcaaatacaggacgaaagggttttgctatgggtggccgaaagaggaaagaccagttagtaggtggtcattcaaagaaagaaaagatgagctccgggcgtgctcccaaacgctcctccgtgatgggtccgggttcgacccatcacgcacaatgaataacctttgttggaactgccaagggatttggaacccttggacagttcaggagcttaaagggatgctatcccttaatgttcccaagctggtattcctcagtgaaacaagatgtacggcggaggagatggtgacagtttggaggaggttggggtggaagcatagtttaggtgtggattgtctctttgtgaagagaaagaacgataagggagttagtagggcaggggggttgtgcttactttgggaggaggatgtgactattgatattcaaaccttctctaatggacattttgatgtggttgttggcgaggcctctgacctaaaacgttttcttttacggggtggggatatcaatgagattacgtcaagggatgataaggagggtggggttttgtggaatctcagacaaatggaggacctgagatgcattagagtttgcaagataagactcacaaggcaagccctaaaagattggagttcaaaaaaatttagacacctgacacgagatattgaggagacccagtctaaattggtgcagttctatgatgtgggaattagaggtcaagacgtgtctgaaggagacgccaatgccaggttttttcatcaacaagcttcaaatcggaaatgcaaaaattgtatcaatgggctgttcaatgatgagggttcgtggtgtacagcggatcgacagcttgagcagattgttttgaagtattttggtgggctatttacgtcgtccaaacatgcagactttggggctgttcttgaggctattccaactgtgattactgaggagattaataacaagttagcgaggaaggtggaggatgatgaagtagtggtcaccttgaagcacatgcaccaatcaaagtcccttggacctgacgacttttcaccctttagatatgagaagggcctacgactgtgtcgaatagaagtttctagaagttgtgatgtggaagttggggttctcattttcaaggctattatgttcagaagaggtgcatggaagattaagggggtggaaatatgtactggagctccaattatttcacatcttttctttgcgaatgattcttttattttctgtaaagcggagacgcaggattgtgtgagtttgaagggagttctgcaatcatatgccatgatgtctggacaacaaattaatcttcagaaaagttctatctcattcagcaagaatgtagagttacaagagcaagttaggttggcagagctgttaggggtacaacatgtggagaggcacgataagtatttgggattgccggttgagataagttattcgaaagaggacgcttttgagtacttggctgagagttcaaaagcgcacttcgggttggcgttgatggccaacttttggtggggtgatgaggaggataaaaggaagattcactggctttcttgggatcaactttatgctctgaaaagagagggagggatgggttttagggacatgcatgactttaacctagctttgttggccaaacaagggtggcggataatcacaaggctcgaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

929

Amino Acids

105.26

Weight (kDa)

7.93

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000619)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29861 FvH4_3g26084 FvH4_3g32551 FvH4_4g08193 FvH4_4g12950 FvH4_6g29733
rosa_chinensis RchiOBHm_Chr3g0488921
rosa_multiflora Rmu_co8238041.1_g000001 Rmu_co8265215.1_g000001 Rmu_co8486357.1_g000002 Rmu_sc0000389.1_g000016 Rmu_sc0000580.1_g000123 Rmu_sc0000789.1_g000005 Rmu_sc0001043.1_g000006 Rmu_sc0001208.1_g000001 Rmu_sc0001323.1_g000011 Rmu_sc0001636.1_g000001 Rmu_sc0001911.1_g000023 Rmu_sc0001940.1_g000010 Rmu_sc0002531.1_g000047 Rmu_sc0002715.1_g000007 Rmu_sc0002833.1_g000024 Rmu_sc0003859.1_g000046 Rmu_sc0003894.1_g000008 Rmu_sc0004744.1_g000008 Rmu_sc0004924.1_g000016 Rmu_sc0004991.1_g000004 Rmu_sc0005149.1_g000010 Rmu_sc0005149.1_g000011 Rmu_sc0005489.1_g000007 Rmu_sc0005592.1_g000030 Rmu_sc0005813.1_g000010 Rmu_sc0005813.1_g000011 Rmu_sc0005861.1_g000009 Rmu_sc0005949.1_g000016 Rmu_sc0006824.1_g000021 Rmu_sc0007069.1_g000025 Rmu_sc0008002.1_g000008 Rmu_sc0008148.1_g000008 Rmu_sc0008280.1_g000001 Rmu_sc0008317.1_g000010 Rmu_sc0011615.1_g000002 Rmu_sc0013925.1_g000004 Rmu_sc0014130.1_g000003 Rmu_sc0014510.1_g000008 Rmu_sc0016640.1_g000001 Rmu_sc0018068.1_g000002 Rmu_sc0021371.1_g000006 Rmu_sc0028652.1_g000007 Rmu_sc0032187.1_g000002 Rmu_sc0034241.1_g000001 Rmu_ssc0000127.1_g000003
rosa_roxburghii Rroxscaffold_6G00403950
rosa_rugosa Rorug01G0081700 Rorug02G0544000 Rorug05G0089900 Rorug05G0284500 Rorug05G0452400 Rorug07G0003400 Rorug07G0003400 Rorug07G0129200 Rorug07G0210600
rosa_samantha Rh1AG193900 Rh1AG194000 Rh1DG063400 Rh1DG204700 Rh2AG364300 Rh3BG140200 Rh3CG366000 Rh4DG342600 Rh5AG347700 Rh5BG437300 Rh5CG459900 Rh5DG127400 Rh5DG412800 Rh6BG381500 Rh7CG520500 Rh7DG241900 Rh7DG336000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 700
AccB7I CCANNNNNTGG 2 cut(s) 914, 2628
AccBSI CCGCTC 1 cut(s) 556
AccII CGCG 1 cut(s) 781
AccIII TCCGGA 1 cut(s) 715
AclI AACGTT 1 cut(s) 1582
AclWI GGATC 4 cut(s) 366, 404, 1944, 2685
AcoI YGGCCR 3 cut(s) 1139, 2619, 2751
AcsI RAATTY 2 cut(s) 104, 1742
AcuI CTGAAG 3 cut(s) 62, 1851, 2408
AcyI GRCGYC 1 cut(s) 1839
AfaI GTAC 5 cut(s) 1372, 1930, 2287, 2499, 2576
AfiI CCNNNNNNNGG 9 cut(s) 151, 498, 577, 731, 914, 966, 1848, 2628, 2760
AflIII ACRYGT 4 cut(s) 224, 367, 1824, 2503
AhdI GACNNNNNGTC 1 cut(s) 663
AjiI CACGTC 1 cut(s) 1825
AjnI CCWGG 6 cut(s) 57, 69, 404, 774, 989, 1847
AjuI GAANNNNNNNTTGG 2 cut(s) 1334, 1366
Alw21I GWGCWC 4 cut(s) 232, 1197, 1208, 2296
Alw26I GTCTC 6 cut(s) 550, 680, 1442, 1766, 1830, 2345
AlwI GGATC 4 cut(s) 366, 404, 1944, 2685
AlwNI CAGNNNCTG 2 cut(s) 344, 405
Aor13HI TCCGGA 1 cut(s) 715
ApeKI GCWGC 3 cut(s) 332, 335, 338
ApoI RAATTY 2 cut(s) 104, 1742
AseI ATTAAT 3 cut(s) 876, 2052, 2418
AspLEI GCGC 4 cut(s) 157, 325, 781, 2599
AspS9I GGNCC 9 cut(s) 165, 183, 402, 987, 1028, 1230, 1672, 2132, 2167
AsuC2I CCSGG 3 cut(s) 839, 1199, 1234
AsuHPI GGTGA 6 cut(s) 215, 497, 1399, 2089, 2139, 2648
AsuII TTCGAA 1 cut(s) 2555
AvaII GGWCC 5 cut(s) 165, 183, 1230, 1672, 2132
BalI TGGCCA 2 cut(s) 2621, 2753
BanII GRGCYC 4 cut(s) 178, 839, 1197, 2296
BauI CACGAG 1 cut(s) 1758
Bbv12I GWGCWC 4 cut(s) 232, 1197, 1208, 2296
BbvI GCAGC 3 cut(s) 322, 344, 350
BceAI ACGGC 1 cut(s) 1389
BciT130I CCWGG 6 cut(s) 59, 71, 406, 776, 991, 1849
BciVI GTATCC 1 cut(s) 1075
BcnI CCSGG 3 cut(s) 839, 1199, 1234
BcoDI GTCTC 6 cut(s) 550, 680, 1442, 1766, 1830, 2345
BfaI CTAG 4 cut(s) 233, 477, 2196, 2741
BfmI CTRYAG 1 cut(s) 336
BfoI RGCGCY 2 cut(s) 158, 326
BfuAI ACCTGC 1 cut(s) 700
BfuI GTATCC 1 cut(s) 1075
BisI GCNGC 3 cut(s) 333, 336, 339
BlsI GCNGC 3 cut(s) 334, 337, 340
BmcAI AGTACT 1 cut(s) 2576
Bme1390I CCNGG 9 cut(s) 59, 71, 406, 776, 839, 991, 1199, 1234, 1849
Bme18I GGWCC 5 cut(s) 165, 183, 1230, 1672, 2132
BmeRI GACNNNNNGTC 1 cut(s) 663
BmgBI CACGTC 1 cut(s) 1825
BmgT120I GGNCC 9 cut(s) 165, 183, 402, 987, 1028, 1230, 1672, 2132, 2167
BmiI GGNNCC 6 cut(s) 166, 185, 514, 714, 1231, 1292
BmrFI CCNGG 9 cut(s) 59, 71, 406, 776, 839, 991, 1199, 1234, 1849
BmrI ACTGGG 1 cut(s) 1771
BmsI GCATC 3 cut(s) 290, 1311, 1670
BmuI ACTGGG 1 cut(s) 1771
BpmI CTGGAG 1 cut(s) 2310
Bpu14I TTCGAA 1 cut(s) 2555
BpuEI CTTGAG 2 cut(s) 1967, 2039
BpuMI CCSGG 3 cut(s) 839, 1199, 1234
BsaHI GRCGYC 1 cut(s) 1839
BsaI GGTCTC 2 cut(s) 680, 1766
BsaJI CCNNGG 9 cut(s) 57, 188, 305, 405, 571, 990, 1279, 1295, 2128
BsaWI WCCGGW 1 cut(s) 715
BsaXI ACNNNNNCTCC 6 cut(s) 627, 657, 1763, 1793, 2278, 2308
Bsc4I CCNNNNNNNGG 9 cut(s) 151, 498, 577, 731, 914, 966, 1848, 2628, 2760
Bse118I RCCGGY 1 cut(s) 2536
Bse1I ACTGG 5 cut(s) 664, 1157, 1777, 2293, 2669
Bse3DI GCAATG 1 cut(s) 835
BseAI TCCGGA 1 cut(s) 715
BseBI CCWGG 6 cut(s) 59, 71, 406, 776, 991, 1849
BseDI CCNNGG 9 cut(s) 57, 188, 305, 405, 571, 990, 1279, 1295, 2128
BseLI CCNNNNNNNGG 9 cut(s) 151, 498, 577, 731, 914, 966, 1848, 2628, 2760
BseMI GCAATG 1 cut(s) 835
BseMII CTCAG 6 cut(s) 345, 1369, 1667, 1672, 2034, 2574
BseNI ACTGG 5 cut(s) 664, 1157, 1777, 2293, 2669
BseRI GAGGAG 9 cut(s) 23, 446, 1208, 1394, 1414, 1517, 1784, 2060, 2657
BseXI GCAGC 3 cut(s) 322, 344, 350
BseYI CCCAGC 1 cut(s) 824
BsgI GTGCAG 1 cut(s) 1811
Bsh1236I CGCG 1 cut(s) 781
Bsh1285I CGRYCG 2 cut(s) 746, 785
BsiEI CGRYCG 2 cut(s) 746, 785
BsiHKAI GWGCWC 4 cut(s) 232, 1197, 1208, 2296
BsiSI CCGG 6 cut(s) 716, 839, 1079, 1198, 1233, 2537
BslFI GGGAC 6 cut(s) 169, 178, 364, 830, 2110, 2735
BslI CCNNNNNNNGG 9 cut(s) 151, 498, 577, 731, 914, 966, 1848, 2628, 2760
BsmAI GTCTC 6 cut(s) 550, 680, 1442, 1766, 1830, 2345
BsmBI CGTCTC 2 cut(s) 1830, 2345
BsmFI GGGAC 6 cut(s) 169, 178, 364, 830, 2110, 2735
BsmI GAATGC 1 cut(s) 463
Bso31I GGTCTC 2 cut(s) 680, 1766
Bsp119I TTCGAA 1 cut(s) 2555
Bsp1286I GDGCHC 6 cut(s) 178, 232, 839, 1197, 1208, 2296
Bsp13I TCCGGA 1 cut(s) 715
Bsp1407I TGTACA 1 cut(s) 1928
Bsp143I GATC 6 cut(s) 277, 358, 396, 743, 1936, 2677
BspCNI CTCAG 6 cut(s) 346, 1368, 1668, 1671, 2035, 2575
BspEI TCCGGA 1 cut(s) 715
BspFNI CGCG 1 cut(s) 781
BspLI GGNNCC 6 cut(s) 166, 185, 514, 714, 1231, 1292
BspMAI CTGCAG 1 cut(s) 340
BspMI ACCTGC 1 cut(s) 700
BspPI GGATC 4 cut(s) 366, 404, 1944, 2685
BspT104I TTCGAA 1 cut(s) 2555
BspTNI GGTCTC 2 cut(s) 680, 1766
BsrBI CCGCTC 1 cut(s) 556
BsrDI GCAATG 1 cut(s) 835
BsrFI RCCGGY 1 cut(s) 2536
BsrGI TGTACA 1 cut(s) 1928
BsrI ACTGG 5 cut(s) 664, 1157, 1777, 2293, 2669
BssAI RCCGGY 1 cut(s) 2536
BssECI CCNNGG 9 cut(s) 57, 188, 305, 405, 571, 990, 1279, 1295, 2128
BssMI GATC 6 cut(s) 277, 358, 396, 743, 1936, 2677
BssNI GRCGYC 1 cut(s) 1839
BssSI CACGAG 1 cut(s) 1758
BssT1I CCWWGG 5 cut(s) 188, 305, 1279, 1295, 2128
Bst2BI CACGAG 1 cut(s) 1758
Bst2UI CCWGG 6 cut(s) 59, 71, 406, 776, 991, 1849
Bst4CI ACNGT 5 cut(s) 803, 1303, 1393, 2035, 2178
Bst6I CTCTTC 2 cut(s) 1442, 2245
BstACI GRCGYC 1 cut(s) 1839
BstAUI TGTACA 1 cut(s) 1928
BstBI TTCGAA 1 cut(s) 2555
BstC8I GCNNGC 4 cut(s) 707, 809, 1204, 1716
BstDEI CTNAG 6 cut(s) 354, 1355, 1658, 1676, 2043, 2583
BstEII GGTNACC 2 cut(s) 580, 2095
BstENI CCTNNNNNAGG 1 cut(s) 575
BstFNI CGCG 1 cut(s) 781
BstH2I RGCGCY 2 cut(s) 158, 326
BstHHI GCGC 4 cut(s) 157, 325, 781, 2599
BstKTI GATC 6 cut(s) 280, 361, 399, 746, 1939, 2680
BstMAI GTCTC 6 cut(s) 550, 680, 1442, 1766, 1830, 2345
BstMBI GATC 6 cut(s) 277, 358, 396, 743, 1936, 2677
BstMCI CGRYCG 2 cut(s) 746, 785
BstMWI GCNNNNNNNGC 5 cut(s) 329, 338, 834, 2618, 2750
BstNI CCWGG 6 cut(s) 59, 71, 406, 776, 991, 1849
BstNSI RCATGY 6 cut(s) 371, 709, 2000, 2113, 2507, 2728
BstPI GGTNACC 2 cut(s) 580, 2095
BstSCI CCNGG 9 cut(s) 57, 69, 404, 774, 837, 989, 1197, 1232, 1847
BstSFI CTRYAG 1 cut(s) 336
BstUI CGCG 1 cut(s) 781
BstV1I GCAGC 3 cut(s) 322, 344, 350
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuI GTATCC 1 cut(s) 1075
BtgZI GCGATG 1 cut(s) 402
BtrI CACGTC 1 cut(s) 1825
BtsIMutI CAGTG 2 cut(s) 1363, 2662
BveI ACCTGC 1 cut(s) 700
Cac8I GCNNGC 4 cut(s) 707, 809, 1204, 1716
CaiI CAGNNNCTG 2 cut(s) 344, 405
CfoI GCGC 4 cut(s) 157, 325, 781, 2599
Cfr10I RCCGGY 1 cut(s) 2536
Cfr13I GGNCC 9 cut(s) 165, 183, 402, 987, 1028, 1230, 1672, 2132, 2167
CseI GACGC 3 cut(s) 1847, 2362, 2573
Csp6I GTAC 5 cut(s) 1371, 1929, 2286, 2498, 2575
CviQI GTAC 5 cut(s) 1371, 1929, 2286, 2498, 2575
DdeI CTNAG 6 cut(s) 354, 1355, 1658, 1676, 2043, 2583
DpnI GATC 6 cut(s) 279, 360, 398, 745, 1938, 2679
DpnII GATC 6 cut(s) 277, 358, 396, 743, 1936, 2677
DriI GACNNNNNGTC 1 cut(s) 663
EaeI YGGCCR 3 cut(s) 1139, 2619, 2751
Eam1104I CTCTTC 2 cut(s) 1442, 2245
Eam1105I GACNNNNNGTC 1 cut(s) 663
EarI CTCTTC 2 cut(s) 1442, 2245
EciI GGCGGA 4 cut(s) 806, 947, 1391, 2782
Ecl136II GAGCTC 2 cut(s) 1195, 2294
Eco130I CCWWGG 5 cut(s) 188, 305, 1279, 1295, 2128
Eco147I AGGCCT 1 cut(s) 1568
Eco24I GRGCYC 4 cut(s) 178, 839, 1197, 2296
Eco31I GGTCTC 2 cut(s) 680, 1766
Eco32I GATATC 1 cut(s) 1606
Eco47I GGWCC 5 cut(s) 165, 183, 1230, 1672, 2132
Eco53kI GAGCTC 2 cut(s) 1195, 2294
Eco57I CTGAAG 3 cut(s) 62, 1851, 2408
Eco91I GGTNACC 2 cut(s) 580, 2095
EcoICRI GAGCTC 2 cut(s) 1195, 2294
EcoNI CCTNNNNNAGG 1 cut(s) 575
EcoO109I RGGNCCY 3 cut(s) 402, 1672, 2167
EcoO65I GGTNACC 2 cut(s) 580, 2095
EcoRII CCWGG 6 cut(s) 57, 69, 404, 774, 989, 1847
EcoRV GATATC 1 cut(s) 1606
EcoT14I CCWWGG 5 cut(s) 188, 305, 1279, 1295, 2128
EcoT22I ATGCAT 2 cut(s) 1685, 2730
EcoT38I GRGCYC 4 cut(s) 178, 839, 1197, 2296
ErhI CCWWGG 5 cut(s) 188, 305, 1279, 1295, 2128
Esp3I CGTCTC 2 cut(s) 1830, 2345
FaqI GGGAC 6 cut(s) 169, 178, 364, 830, 2110, 2735
FauI CCCGC 1 cut(s) 912
FauNDI CATATG 1 cut(s) 2392
Fnu4HI GCNGC 3 cut(s) 333, 336, 339
FriOI GRGCYC 4 cut(s) 178, 839, 1197, 2296
Fsp4HI GCNGC 3 cut(s) 333, 336, 339
FspBI CTAG 4 cut(s) 233, 477, 2196, 2741
GlaI GCGC 4 cut(s) 156, 324, 780, 2598
GluI GCNGC 3 cut(s) 333, 336, 339
GsaI CCCAGC 1 cut(s) 828
GsuI CTGGAG 1 cut(s) 2310
HaeII RGCGCY 2 cut(s) 158, 326
HapII CCGG 6 cut(s) 716, 839, 1079, 1198, 1233, 2537
HgaI GACGC 3 cut(s) 1847, 2362, 2573
HhaI GCGC 4 cut(s) 157, 325, 781, 2599
Hin1I GRCGYC 1 cut(s) 1839
Hin6I GCGC 4 cut(s) 155, 323, 779, 2597
HinP1I GCGC 4 cut(s) 155, 323, 779, 2597
HindIII AAGCTT 1 cut(s) 1866
HinfI GANTC 8 cut(s) 37, 208, 624, 1049, 1654, 1704, 2327, 2659
HpaII CCGG 6 cut(s) 716, 839, 1079, 1198, 1233, 2537
HphI GGTGA 6 cut(s) 215, 497, 1399, 2089, 2139, 2648
Hpy166II GTNNAC 3 cut(s) 657, 799, 1929
Hpy188III TCNNGA 8 cut(s) 233, 716, 747, 1307, 1820, 2018, 2196, 2406
Hpy8I GTNNAC 3 cut(s) 657, 799, 1929
Hpy99I CGWCG 1 cut(s) 1990
HpyCH4III ACNGT 5 cut(s) 803, 1303, 1393, 2035, 2178
HpyCH4IV ACGT 5 cut(s) 224, 1582, 1619, 1824, 1985
HpyF10VI GCNNNNNNNGC 5 cut(s) 329, 338, 834, 2618, 2750
HpyF3I CTNAG 6 cut(s) 354, 1355, 1658, 1676, 2043, 2583
HpySE526I ACGT 5 cut(s) 224, 1582, 1619, 1824, 1985
Hsp92I GRCGYC 1 cut(s) 1839
HspAI GCGC 4 cut(s) 155, 323, 779, 2597
Kpn2I TCCGGA 1 cut(s) 715
Kzo9I GATC 6 cut(s) 277, 358, 396, 743, 1936, 2677
LmnI GCTCC 8 cut(s) 240, 869, 1200, 1211, 1221, 1309, 2291, 2299
Lsp1109I GCAGC 3 cut(s) 322, 344, 350
LweI GCATC 3 cut(s) 290, 1311, 1670
MaeI CTAG 4 cut(s) 233, 477, 2196, 2741
MaeII ACGT 5 cut(s) 224, 1582, 1619, 1824, 1985
MaeIII GTNAC 6 cut(s) 580, 888, 1387, 1510, 2095, 2460
MalI GATC 6 cut(s) 279, 360, 398, 745, 1938, 2679
MbiI CCGCTC 1 cut(s) 556
MboI GATC 6 cut(s) 277, 358, 396, 743, 1936, 2677
MboII GAAGA 7 cut(s) 17, 907, 1459, 2262, 2275, 2414, 2668
MfeI CAATTG 1 cut(s) 530
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 6 cut(s) 178, 232, 839, 1197, 1208, 2296
MlsI TGGCCA 2 cut(s) 2621, 2753
MluNI TGGCCA 2 cut(s) 2621, 2753
MlyI GAGTC 3 cut(s) 31, 633, 1698
MmeI TCCRAC 4 cut(s) 607, 1002, 1250, 2054
Mox20I TGGCCA 2 cut(s) 2621, 2753
Mph1103I ATGCAT 2 cut(s) 1685, 2730
MroI TCCGGA 1 cut(s) 715
MscI TGGCCA 2 cut(s) 2621, 2753
MseI TTAA 8 cut(s) 876, 954, 1314, 1332, 2052, 2268, 2418, 2736
MslI CAYNNNNRTG 1 cut(s) 1548
Msp20I TGGCCA 2 cut(s) 2621, 2753
MspA1I CMGCKG 2 cut(s) 335, 1934
MspI CCGG 6 cut(s) 716, 839, 1079, 1198, 1233, 2537
MspR9I CCNGG 9 cut(s) 59, 71, 406, 776, 839, 991, 1199, 1234, 1849
MunI CAATTG 1 cut(s) 530
Mva1269I GAATGC 1 cut(s) 463
MvaI CCWGG 6 cut(s) 59, 71, 406, 776, 991, 1849
MvnI CGCG 1 cut(s) 781
MwoI GCNNNNNNNGC 5 cut(s) 329, 338, 834, 2618, 2750
NciI CCSGG 3 cut(s) 839, 1199, 1234
NdeI CATATG 1 cut(s) 2392
NdeII GATC 6 cut(s) 277, 358, 396, 743, 1936, 2677
NlaIV GGNNCC 6 cut(s) 166, 185, 514, 714, 1231, 1292
NmuCI GTSAC 3 cut(s) 1387, 1510, 2095
NsiI ATGCAT 2 cut(s) 1685, 2730
NspI RCATGY 6 cut(s) 371, 709, 2000, 2113, 2507, 2728
NspV TTCGAA 1 cut(s) 2555
PaeI GCATGC 1 cut(s) 709
PceI AGGCCT 1 cut(s) 1568
PciI ACATGT 2 cut(s) 367, 2503
PcsI WCGNNNNNNNCGW 1 cut(s) 2179
PctI GAATGC 1 cut(s) 463
PfeI GAWTC 5 cut(s) 208, 1049, 1654, 2327, 2659
PflFI GACNNNGTC 2 cut(s) 1777, 2178
PflMI CCANNNNNTGG 2 cut(s) 914, 2628
PkrI GCNGC 3 cut(s) 334, 337, 340
Ple19I CGATCG 1 cut(s) 746
PleI GAGTC 3 cut(s) 31, 632, 1698
PpsI GAGTC 3 cut(s) 31, 632, 1698
PpuMI RGGWCCY 1 cut(s) 1672
PscI ACATGT 2 cut(s) 367, 2503
PshBI ATTAAT 3 cut(s) 876, 2052, 2418
Psp124BI GAGCTC 2 cut(s) 1197, 2296
Psp1406I AACGTT 1 cut(s) 1582
Psp5II RGGWCCY 1 cut(s) 1672
Psp6I CCWGG 6 cut(s) 57, 69, 404, 774, 989, 1847
PspEI GGTNACC 2 cut(s) 580, 2095
PspFI CCCAGC 1 cut(s) 824
PspGI CCWGG 6 cut(s) 57, 69, 404, 774, 989, 1847
PspN4I GGNNCC 6 cut(s) 166, 185, 514, 714, 1231, 1292
PspPI GGNCC 9 cut(s) 165, 183, 402, 987, 1028, 1230, 1672, 2132, 2167
PspPPI RGGWCCY 1 cut(s) 1672
PstI CTGCAG 1 cut(s) 340
PstNI CAGNNNCTG 2 cut(s) 344, 405
PsuI RGATCY 1 cut(s) 358
PsyI GACNNNGTC 2 cut(s) 1777, 2178
PvuI CGATCG 1 cut(s) 746
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 5 cut(s) 1372, 1930, 2287, 2499, 2576
RsaNI GTAC 5 cut(s) 1371, 1929, 2286, 2498, 2575
RseI CAYNNNNRTG 1 cut(s) 1548
SacI GAGCTC 2 cut(s) 1197, 2296
SaqAI TTAA 8 cut(s) 876, 954, 1314, 1332, 2052, 2268, 2418, 2736
SatI GCNGC 3 cut(s) 333, 336, 339
Sau3AI GATC 6 cut(s) 277, 358, 396, 743, 1936, 2677
Sau96I GGNCC 9 cut(s) 165, 183, 402, 987, 1028, 1230, 1672, 2132, 2167
ScaI AGTACT 1 cut(s) 2576
SchI GAGTC 3 cut(s) 31, 633, 1698
ScrFI CCNGG 9 cut(s) 59, 71, 406, 776, 839, 991, 1199, 1234, 1849
SduI GDGCHC 6 cut(s) 178, 232, 839, 1197, 1208, 2296
SfaNI GCATC 3 cut(s) 290, 1311, 1670
SfcI CTRYAG 1 cut(s) 336
SfuI TTCGAA 1 cut(s) 2555
SinI GGWCC 5 cut(s) 165, 183, 1230, 1672, 2132
SmiMI CAYNNNNRTG 1 cut(s) 1548
SmlI CTYRAG 2 cut(s) 1946, 2018
SmoI CTYRAG 2 cut(s) 1946, 2018
SphI GCATGC 1 cut(s) 709
SseBI AGGCCT 1 cut(s) 1568
SspMI CTAG 4 cut(s) 233, 477, 2196, 2741
SstI GAGCTC 2 cut(s) 1197, 2296
StuI AGGCCT 1 cut(s) 1568
StyD4I CCNGG 9 cut(s) 57, 69, 404, 774, 837, 989, 1197, 1232, 1847
StyI CCWWGG 5 cut(s) 188, 305, 1279, 1295, 2128
TaaI ACNGT 5 cut(s) 803, 1303, 1393, 2035, 2178
TaiI ACGT 5 cut(s) 227, 1585, 1622, 1827, 1988
TaqII GACCGA 1 cut(s) 563
TatI WGTACW 3 cut(s) 1928, 2285, 2574
TfiI GAWTC 5 cut(s) 208, 1049, 1654, 2327, 2659
Tru1I TTAA 8 cut(s) 876, 954, 1314, 1332, 2052, 2268, 2418, 2736
Tru9I TTAA 8 cut(s) 876, 954, 1314, 1332, 2052, 2268, 2418, 2736
TscAI CASTG 2 cut(s) 1363, 2669
TseFI GTSAC 3 cut(s) 1387, 1510, 2095
TseI GCWGC 3 cut(s) 332, 335, 338
Tsp45I GTSAC 3 cut(s) 1387, 1510, 2095
TspDTI ATGAA 3 cut(s) 1271, 1847, 2100
TspGWI ACGGA 2 cut(s) 95, 1211
TspRI CASTG 2 cut(s) 1363, 2669
Tth111I GACNNNGTC 2 cut(s) 1777, 2178
Van91I CCANNNNNTGG 2 cut(s) 914, 2628
VpaK11BI GGWCC 5 cut(s) 165, 183, 1230, 1672, 2132
VspI ATTAAT 3 cut(s) 876, 2052, 2418
XagI CCTNNNNNAGG 1 cut(s) 575
XapI RAATTY 2 cut(s) 104, 1742
XbaI TCTAGA 2 cut(s) 232, 2195
XceI RCATGY 6 cut(s) 371, 709, 2000, 2113, 2507, 2728
XcmI CCANNNNNNNNNTGG 1 cut(s) 1164
XspI CTAG 4 cut(s) 233, 477, 2196, 2741
ZrmI AGTACT 1 cut(s) 2576
Zsp2I ATGCAT 2 cut(s) 1685, 2730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.