Rmu_sc0004991.1_g000004

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004991.1
OG0000619
Physical Location & Seq
Reverse (-)
10784 .. 11844
1061 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004991.1_g000004.1.cds

Sequence Viewer

Length: 981 bp
atggttagggatcgtgctgagtgggaggaagatggtggggaggatatggggcaggatggcatgtgggtagatagtgtgtcggcaaggcaggtggaggggacgaggacggggaagcggcggcggacggtggcggatgaggtgactgatctgattatggagggttgtatttcaggaaagtctgtcaatcataattctttggagttaatgggagcgctaactgcgctaacatgctcacaaggtgatcaagaattaaatttgaaggatgtcattgatcctggaactatatgttcgaagtcattgccgagggaggtgatttctccgaatagattggagcctttagcggattccgaagttcctggagttacggcggaaagagtggactgtttgggagatgggtcccaagtgcgggatcgcagcgagactaaccctatgggccttgttccagaggagggggagagcgagttggatgtagagtgtgggcctggtaatcagtgtaggggtcaaccaaagtccgggctggcccctaagtccctaaggcctactttactgaataggaagaagagttccaaatggggtgttaaggtgggtggcagcaggaggaaaggtgttgttaggattccaaactcttcgttgaaagatgttttgagcaatgaacgtgctcctgaacgttcctccgtgatggggcttaaaggggtagtttccctcaacattcccaacgttatttttcttagtgagactaggtgtacggcggaggagatggatggtgttcggagacaacttgggtggaagaatgcggtgtcagtgaattgcaaatttgtaaagaagaaaaagggaaagggagttagtagggcggggggtttatgtctgctttggaatgaggatgtcaaagtgtcactacaatcatattcggagaatcatattgacgtcgtaattggtgagacgggggatcctggttgttggcggtttactggcttgtattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

326

Amino Acids

35.47

Weight (kDa)

6.28

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000619)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29861 FvH4_3g26084 FvH4_3g32551 FvH4_4g08193 FvH4_4g12950 FvH4_6g29733
rosa_chinensis RchiOBHm_Chr3g0488921
rosa_multiflora Rmu_co8238041.1_g000001 Rmu_co8265215.1_g000001 Rmu_co8486357.1_g000002 Rmu_sc0000389.1_g000016 Rmu_sc0000580.1_g000123 Rmu_sc0000789.1_g000005 Rmu_sc0001043.1_g000006 Rmu_sc0001208.1_g000001 Rmu_sc0001323.1_g000011 Rmu_sc0001636.1_g000001 Rmu_sc0001911.1_g000023 Rmu_sc0001940.1_g000010 Rmu_sc0002531.1_g000047 Rmu_sc0002715.1_g000007 Rmu_sc0002833.1_g000024 Rmu_sc0003859.1_g000046 Rmu_sc0003894.1_g000008 Rmu_sc0004744.1_g000008 Rmu_sc0004924.1_g000016 Rmu_sc0004991.1_g000004 Rmu_sc0005149.1_g000010 Rmu_sc0005149.1_g000011 Rmu_sc0005489.1_g000007 Rmu_sc0005592.1_g000030 Rmu_sc0005813.1_g000010 Rmu_sc0005813.1_g000011 Rmu_sc0005861.1_g000009 Rmu_sc0005949.1_g000016 Rmu_sc0006824.1_g000021 Rmu_sc0007069.1_g000025 Rmu_sc0008002.1_g000008 Rmu_sc0008148.1_g000008 Rmu_sc0008280.1_g000001 Rmu_sc0008317.1_g000010 Rmu_sc0011615.1_g000002 Rmu_sc0013925.1_g000004 Rmu_sc0014130.1_g000003 Rmu_sc0014510.1_g000008 Rmu_sc0016640.1_g000001 Rmu_sc0018068.1_g000002 Rmu_sc0021371.1_g000006 Rmu_sc0028652.1_g000007 Rmu_sc0032187.1_g000002 Rmu_sc0034241.1_g000001 Rmu_ssc0000127.1_g000003
rosa_roxburghii Rroxscaffold_6G00403950
rosa_rugosa Rorug01G0081700 Rorug02G0544000 Rorug05G0089900 Rorug05G0284500 Rorug05G0452400 Rorug07G0003400 Rorug07G0003400 Rorug07G0129200 Rorug07G0210600
rosa_samantha Rh1AG193900 Rh1AG194000 Rh1DG063400 Rh1DG204700 Rh2AG364300 Rh3BG140200 Rh3CG366000 Rh4DG342600 Rh5AG347700 Rh5BG437300 Rh5CG459900 Rh5DG127400 Rh5DG412800 Rh6BG381500 Rh7CG520500 Rh7DG241900 Rh7DG336000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 79
AatII GACGTC 1 cut(s) 927
Acc36I ACCTGC 1 cut(s) 79
AclI AACGTT 2 cut(s) 667, 717
AclWI GGATC 5 cut(s) 18, 266, 417, 941, 954
AcsI RAATTY 2 cut(s) 253, 812
AcyI GRCGYC 1 cut(s) 924
AdeI CACNNNGTG 1 cut(s) 239
AfaI GTAC 1 cut(s) 745
AfeI AGCGCT 1 cut(s) 213
AfiI CCNNNNNNNGG 5 cut(s) 405, 406, 449, 512, 681
AgsI TTSAA 2 cut(s) 259, 634
AjnI CCWGG 4 cut(s) 274, 355, 481, 949
AloI GAACNNNNNNTCC 2 cut(s) 547, 579
Alw21I GWGCWC 1 cut(s) 661
Alw26I GTCTC 4 cut(s) 413, 728, 766, 932
AlwI GGATC 5 cut(s) 18, 266, 417, 941, 954
Aor51HI AGCGCT 1 cut(s) 213
AoxI GGCC 4 cut(s) 433, 479, 519, 536
ApeKI GCWGC 2 cut(s) 414, 591
ApoI RAATTY 2 cut(s) 253, 812
AspLEI GCGC 2 cut(s) 214, 223
AspS9I GGNCC 4 cut(s) 396, 433, 479, 520
AsuC2I CCSGG 1 cut(s) 514
AsuHPI GGTGA 4 cut(s) 151, 251, 322, 947
AsuII TTCGAA 1 cut(s) 290
AvaII GGWCC 1 cut(s) 396
AxyI CCTNAGG 1 cut(s) 533
BamHI GGATCC 1 cut(s) 946
Bbv12I GWGCWC 1 cut(s) 661
BbvI GCAGC 2 cut(s) 426, 603
BccI CCATC 6 cut(s) 26, 50, 386, 673, 751, 755
BceAI ACGGC 2 cut(s) 381, 762
BcgI CGANNNNNNTGC 2 cut(s) 280, 314
BciT130I CCWGG 4 cut(s) 276, 357, 483, 951
BclI TGATCA 1 cut(s) 241
BcnI CCSGG 1 cut(s) 514
BcoDI GTCTC 4 cut(s) 413, 728, 766, 932
BfaI CTAG 1 cut(s) 738
BfoI RGCGCY 1 cut(s) 215
BfuAI ACCTGC 1 cut(s) 79
BisI GCNGC 4 cut(s) 116, 119, 415, 592
BlsI GCNGC 4 cut(s) 117, 120, 416, 593
Bme1390I CCNGG 5 cut(s) 276, 357, 483, 514, 951
Bme18I GGWCC 1 cut(s) 396
BmgT120I GGNCC 4 cut(s) 396, 433, 479, 520
BmiI GGNNCC 5 cut(s) 333, 397, 398, 522, 948
BmrFI CCNGG 5 cut(s) 276, 357, 483, 514, 951
BpmI CTGGAG 1 cut(s) 378
Bpu14I TTCGAA 1 cut(s) 290
BpuMI CCSGG 1 cut(s) 514
BsaHI GRCGYC 1 cut(s) 924
BsaJI CCNNGG 1 cut(s) 302
BsaXI ACNNNNNCTCC 4 cut(s) 446, 476, 589, 619
Bsc4I CCNNNNNNNGG 5 cut(s) 405, 406, 449, 512, 681
Bse1I ACTGG 1 cut(s) 973
Bse21I CCTNAGG 1 cut(s) 533
Bse3DI GCAATG 2 cut(s) 296, 655
BseBI CCWGG 4 cut(s) 276, 357, 483, 951
BseDI CCNNGG 1 cut(s) 302
BseGI GGATG 6 cut(s) 61, 139, 268, 472, 766, 886
BseLI CCNNNNNNNGG 5 cut(s) 405, 406, 449, 512, 681
BseMI GCAATG 2 cut(s) 296, 655
BseMII CTCAG 1 cut(s) 9
BseNI ACTGG 1 cut(s) 973
BseRI GAGGAG 2 cut(s) 461, 767
BseXI GCAGC 2 cut(s) 426, 603
BshFI GGCC 4 cut(s) 435, 481, 521, 538
BsiHKAI GWGCWC 1 cut(s) 661
BsiSI CCGG 1 cut(s) 513
BslFI GGGAC 3 cut(s) 112, 382, 514
BslI CCNNNNNNNGG 5 cut(s) 405, 406, 449, 512, 681
BsmAI GTCTC 4 cut(s) 413, 728, 766, 932
BsmBI CGTCTC 1 cut(s) 932
BsmFI GGGAC 3 cut(s) 112, 382, 514
BsmI GAATGC 1 cut(s) 796
BsnI GGCC 4 cut(s) 435, 481, 521, 538
Bsp119I TTCGAA 1 cut(s) 290
Bsp1286I GDGCHC 1 cut(s) 661
Bsp143I GATC 6 cut(s) 10, 145, 241, 271, 409, 946
BspANI GGCC 4 cut(s) 435, 481, 521, 538
BspCNI CTCAG 1 cut(s) 10
BspLI GGNNCC 5 cut(s) 333, 397, 398, 522, 948
BspMI ACCTGC 1 cut(s) 79
BspPI GGATC 5 cut(s) 18, 266, 417, 941, 954
BspT104I TTCGAA 1 cut(s) 290
BsrDI GCAATG 2 cut(s) 296, 655
BsrI ACTGG 1 cut(s) 973
BssECI CCNNGG 1 cut(s) 302
BssMI GATC 6 cut(s) 10, 145, 241, 271, 409, 946
BssNI GRCGYC 1 cut(s) 924
Bst2UI CCWGG 4 cut(s) 276, 357, 483, 951
Bst4CI ACNGT 2 cut(s) 127, 383
Bst6I CTCTTC 2 cut(s) 554, 631
BstACI GRCGYC 1 cut(s) 924
BstBI TTCGAA 1 cut(s) 290
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 4 cut(s) 18, 525, 533, 728
BstF5I GGATG 6 cut(s) 61, 139, 268, 472, 766, 886
BstH2I RGCGCY 1 cut(s) 215
BstHHI GCGC 2 cut(s) 214, 223
BstKTI GATC 6 cut(s) 13, 148, 244, 274, 412, 949
BstMAI GTCTC 4 cut(s) 413, 728, 766, 932
BstMBI GATC 6 cut(s) 10, 145, 241, 271, 409, 946
BstMWI GCNNNNNNNGC 2 cut(s) 218, 220
BstNI CCWGG 4 cut(s) 276, 357, 483, 951
BstNSI RCATGY 2 cut(s) 64, 231
BstSCI CCNGG 5 cut(s) 274, 355, 481, 512, 949
BstV1I GCAGC 2 cut(s) 426, 603
BstX2I RGATCY 1 cut(s) 946
BstYI RGATCY 1 cut(s) 946
Bsu36I CCTNAGG 1 cut(s) 533
BsuRI GGCC 4 cut(s) 435, 481, 521, 538
BtsCI GGATG 6 cut(s) 61, 139, 268, 472, 766, 886
BtsIMutI CAGTG 2 cut(s) 497, 807
BveI ACCTGC 1 cut(s) 79
Cac8I GCNNGC 1 cut(s) 519
CfoI GCGC 2 cut(s) 214, 223
Cfr13I GGNCC 4 cut(s) 396, 433, 479, 520
Csp6I GTAC 1 cut(s) 744
CspCI CAANNNNNGTGG 4 cut(s) 72, 107, 764, 799
CviAII CATG 2 cut(s) 61, 228
CviJI RGCY 8 cut(s) 334, 435, 481, 517, 521, 538, 685, 972
CviKI_1 RGCY 8 cut(s) 334, 435, 481, 517, 521, 538, 685, 972
CviQI GTAC 1 cut(s) 744
DdeI CTNAG 4 cut(s) 18, 525, 533, 728
DpnI GATC 6 cut(s) 12, 147, 243, 273, 411, 948
DpnII GATC 6 cut(s) 10, 145, 241, 271, 409, 946
DraIII CACNNNGTG 1 cut(s) 239
Eam1104I CTCTTC 2 cut(s) 554, 631
EarI CTCTTC 2 cut(s) 554, 631
EciI GGCGGA 4 cut(s) 136, 146, 383, 764
Eco147I AGGCCT 1 cut(s) 538
Eco47I GGWCC 1 cut(s) 396
Eco47III AGCGCT 1 cut(s) 213
Eco81I CCTNAGG 1 cut(s) 533
EcoO109I RGGNCCY 1 cut(s) 396
EcoRII CCWGG 4 cut(s) 274, 355, 481, 949
Esp3I CGTCTC 1 cut(s) 932
FaeI CATG 2 cut(s) 64, 231
FalI AAGNNNNNCTT 2 cut(s) 526, 558
FaqI GGGAC 3 cut(s) 112, 382, 514
FatI CATG 2 cut(s) 60, 227
FauI CCCGC 2 cut(s) 399, 844
FbaI TGATCA 1 cut(s) 241
Fnu4HI GCNGC 4 cut(s) 116, 119, 415, 592
FokI GGATG 6 cut(s) 68, 146, 275, 479, 773, 893
Fsp4HI GCNGC 4 cut(s) 116, 119, 415, 592
FspBI CTAG 1 cut(s) 738
GlaI GCGC 2 cut(s) 213, 222
GluI GCNGC 4 cut(s) 116, 119, 415, 592
GsuI CTGGAG 1 cut(s) 378
HaeII RGCGCY 1 cut(s) 215
HaeIII GGCC 4 cut(s) 435, 481, 521, 538
HapII CCGG 1 cut(s) 513
HhaI GCGC 2 cut(s) 214, 223
Hin1I GRCGYC 1 cut(s) 924
Hin1II CATG 2 cut(s) 64, 231
Hin6I GCGC 2 cut(s) 212, 221
HinP1I GCGC 2 cut(s) 212, 221
HincII GTYRAC 1 cut(s) 503
HindII GTYRAC 1 cut(s) 503
HinfI GANTC 3 cut(s) 344, 616, 913
HpaII CCGG 1 cut(s) 513
HphI GGTGA 4 cut(s) 151, 251, 322, 947
Hpy166II GTNNAC 4 cut(s) 379, 503, 744, 966
Hpy188I TCNGA 5 cut(s) 150, 321, 349, 771, 910
Hpy188III TCNNGA 4 cut(s) 171, 245, 443, 662
Hpy8I GTNNAC 4 cut(s) 379, 503, 744, 966
Hpy99I CGWCG 1 cut(s) 929
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 2 cut(s) 127, 383
HpyCH4IV ACGT 4 cut(s) 655, 667, 717, 924
HpyCH4V TGCA 1 cut(s) 810
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 220
HpyF3I CTNAG 4 cut(s) 18, 525, 533, 728
HpySE526I ACGT 4 cut(s) 655, 667, 717, 924
Hsp92I GRCGYC 1 cut(s) 924
Hsp92II CATG 2 cut(s) 64, 231
HspAI GCGC 2 cut(s) 212, 221
KflI GGGWCCC 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 6 cut(s) 10, 145, 241, 271, 409, 946
LmnI GCTCC 3 cut(s) 209, 331, 664
Lsp1109I GCAGC 2 cut(s) 426, 603
MaeI CTAG 1 cut(s) 738
MaeII ACGT 4 cut(s) 655, 667, 717, 924
MaeIII GTNAC 3 cut(s) 139, 361, 891
MalI GATC 6 cut(s) 12, 147, 243, 273, 411, 948
MboI GATC 6 cut(s) 10, 145, 241, 271, 409, 946
MboII GAAGA 6 cut(s) 41, 568, 571, 618, 799, 835
MflI RGATCY 1 cut(s) 946
MhlI GDGCHC 1 cut(s) 661
MluCI AATT 6 cut(s) 190, 248, 253, 805, 812, 930
MmeI TCCRAC 1 cut(s) 444
MseI TTAA 4 cut(s) 203, 251, 579, 687
MspI CCGG 1 cut(s) 513
MspR9I CCNGG 5 cut(s) 276, 357, 483, 514, 951
Mva1269I GAATGC 1 cut(s) 796
MvaI CCWGG 4 cut(s) 276, 357, 483, 951
MwoI GCNNNNNNNGC 2 cut(s) 218, 220
NciI CCSGG 1 cut(s) 514
NdeII GATC 6 cut(s) 10, 145, 241, 271, 409, 946
NlaIII CATG 2 cut(s) 64, 231
NlaIV GGNNCC 5 cut(s) 333, 397, 398, 522, 948
NmeAIII GCCGAG 1 cut(s) 327
NmuCI GTSAC 2 cut(s) 139, 891
NspI RCATGY 2 cut(s) 64, 231
NspV TTCGAA 1 cut(s) 290
PaqCI CACCTGC 1 cut(s) 79
PceI AGGCCT 1 cut(s) 538
PctI GAATGC 1 cut(s) 796
PfeI GAWTC 3 cut(s) 344, 616, 913
PfoI TCCNGGA 2 cut(s) 274, 355
PkrI GCNGC 4 cut(s) 117, 120, 416, 593
PpuMI RGGWCCY 1 cut(s) 396
Psp1406I AACGTT 2 cut(s) 667, 717
Psp5II RGGWCCY 1 cut(s) 396
Psp6I CCWGG 4 cut(s) 274, 355, 481, 949
PspGI CCWGG 4 cut(s) 274, 355, 481, 949
PspN4I GGNNCC 5 cut(s) 333, 397, 398, 522, 948
PspPI GGNCC 4 cut(s) 396, 433, 479, 520
PspPPI RGGWCCY 1 cut(s) 396
PsuI RGATCY 1 cut(s) 946
RsaI GTAC 1 cut(s) 745
RsaNI GTAC 1 cut(s) 744
SaqAI TTAA 4 cut(s) 203, 251, 579, 687
SatI GCNGC 4 cut(s) 116, 119, 415, 592
Sau3AI GATC 6 cut(s) 10, 145, 241, 271, 409, 946
Sau96I GGNCC 4 cut(s) 396, 433, 479, 520
ScrFI CCNGG 5 cut(s) 276, 357, 483, 514, 951
SduI GDGCHC 1 cut(s) 661
SfuI TTCGAA 1 cut(s) 290
SinI GGWCC 1 cut(s) 396
Sse9I AATT 6 cut(s) 190, 248, 253, 805, 812, 930
SseBI AGGCCT 1 cut(s) 538
SspMI CTAG 1 cut(s) 738
StuI AGGCCT 1 cut(s) 538
StyD4I CCNGG 5 cut(s) 274, 355, 481, 512, 949
TaaI ACNGT 2 cut(s) 127, 383
TaiI ACGT 4 cut(s) 658, 670, 720, 927
TaqI TCGA 1 cut(s) 290
TasI AATT 6 cut(s) 190, 248, 253, 805, 812, 930
TauI GCSGC 2 cut(s) 118, 121
TfiI GAWTC 3 cut(s) 344, 616, 913
Tru1I TTAA 4 cut(s) 203, 251, 579, 687
Tru9I TTAA 4 cut(s) 203, 251, 579, 687
TscAI CASTG 2 cut(s) 497, 807
TseFI GTSAC 2 cut(s) 139, 891
TseI GCWGC 2 cut(s) 414, 591
Tsp45I GTSAC 2 cut(s) 139, 891
TspDTI ATGAA 1 cut(s) 666
TspGWI ACGGA 1 cut(s) 664
TspRI CASTG 2 cut(s) 497, 807
VpaK11BI GGWCC 1 cut(s) 396
XapI RAATTY 2 cut(s) 253, 812
XceI RCATGY 2 cut(s) 64, 231
XspI CTAG 1 cut(s) 738
ZraI GACGTC 1 cut(s) 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.