Rmu_sc0000389.1_g000016

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000389.1
OG0000619
Physical Location & Seq
Forward (+)
80016 .. 81837
1822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000389.1_g000016.1.cds

Sequence Viewer

Length: 861 bp
atgggcttggctaaacaaaagggtgaggcctgcttatctgagctggaggcagggggtctgcaactggactattgggtgaaaagcagcccaaaagatcgggtcgcaagaaagttaatgtggctgatcccgctctgggcccagagaacagcccaaaacggaatgggaaatctatggcaaggcatggcaatgctccaagaattgaaggaagggttagaagtgaatcttagaacatactctgacaaccacattgatgtcttcattgggagtgctggggatgaccttcacgattggagatggcctaacaggtggagatttactggtgtgtatggatactcaaaggtggagcttcggcacttaacttgggagcttatcaggaggctagggcttaacaattgctggccctggttaattggcggtgattttaacgagattctcaatgcttgtgagaaggagggaggcctgccaagatgtcctagacagatggaatcgtttagtagatgtgttgatgagtgtgggttgattgatcaccatttcgttggcccgtgcttcacctggagaggaaaacatgccggagaagaaattaagatgcgtctagacagattcctggctactaaaaaatgggtggagtggtttccggtctcaagagttttacatctgaaaccgtctaagtctgaccatctacctttgttgttggaagtacagacacatagacctaagaaaaaggtacggaagaaaagattccaatttgaggaacactggttacaagaattagagtgtgctgaggtggttagacgaggttgggagggggatgatggcagcgaccctttccaaacggtgtgcaggcgtcttgcccaaacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

286

Amino Acids

33.5

Weight (kDa)

8.69

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000619)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29861 FvH4_3g26084 FvH4_3g32551 FvH4_4g08193 FvH4_4g12950 FvH4_6g29733
rosa_chinensis RchiOBHm_Chr3g0488921
rosa_multiflora Rmu_co8238041.1_g000001 Rmu_co8265215.1_g000001 Rmu_co8486357.1_g000002 Rmu_sc0000389.1_g000016 Rmu_sc0000580.1_g000123 Rmu_sc0000789.1_g000005 Rmu_sc0001043.1_g000006 Rmu_sc0001208.1_g000001 Rmu_sc0001323.1_g000011 Rmu_sc0001636.1_g000001 Rmu_sc0001911.1_g000023 Rmu_sc0001940.1_g000010 Rmu_sc0002531.1_g000047 Rmu_sc0002715.1_g000007 Rmu_sc0002833.1_g000024 Rmu_sc0003859.1_g000046 Rmu_sc0003894.1_g000008 Rmu_sc0004744.1_g000008 Rmu_sc0004924.1_g000016 Rmu_sc0004991.1_g000004 Rmu_sc0005149.1_g000010 Rmu_sc0005149.1_g000011 Rmu_sc0005489.1_g000007 Rmu_sc0005592.1_g000030 Rmu_sc0005813.1_g000010 Rmu_sc0005813.1_g000011 Rmu_sc0005861.1_g000009 Rmu_sc0005949.1_g000016 Rmu_sc0006824.1_g000021 Rmu_sc0007069.1_g000025 Rmu_sc0008002.1_g000008 Rmu_sc0008148.1_g000008 Rmu_sc0008280.1_g000001 Rmu_sc0008317.1_g000010 Rmu_sc0011615.1_g000002 Rmu_sc0013925.1_g000004 Rmu_sc0014130.1_g000003 Rmu_sc0014510.1_g000008 Rmu_sc0016640.1_g000001 Rmu_sc0018068.1_g000002 Rmu_sc0021371.1_g000006 Rmu_sc0028652.1_g000007 Rmu_sc0032187.1_g000002 Rmu_sc0034241.1_g000001 Rmu_ssc0000127.1_g000003
rosa_roxburghii Rroxscaffold_6G00403950
rosa_rugosa Rorug01G0081700 Rorug02G0544000 Rorug05G0089900 Rorug05G0284500 Rorug05G0452400 Rorug07G0003400 Rorug07G0003400 Rorug07G0129200 Rorug07G0210600
rosa_samantha Rh1AG193900 Rh1AG194000 Rh1DG063400 Rh1DG204700 Rh2AG364300 Rh3BG140200 Rh3CG366000 Rh4DG342600 Rh5AG347700 Rh5BG437300 Rh5CG459900 Rh5DG127400 Rh5DG412800 Rh6BG381500 Rh7CG520500 Rh7DG241900 Rh7DG336000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 130
AciI CCGC 2 cut(s) 128, 414
AclWI GGATC 1 cut(s) 118
AcyI GRCGYC 1 cut(s) 844
AfaI GTAC 2 cut(s) 699, 726
AfiI CCNNNNNNNGG 2 cut(s) 133, 748
AgsI TTSAA 1 cut(s) 202
AjnI CCWGG 3 cut(s) 401, 551, 603
AluBI AGCT 3 cut(s) 43, 346, 367
AluI AGCT 3 cut(s) 43, 346, 367
Alw26I GTCTC 1 cut(s) 643
AlwI GGATC 1 cut(s) 118
AoxI GGCC 6 cut(s) 27, 135, 296, 398, 457, 538
ApaI GGGCCC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 84, 816
Asp700I GAANNNNTTC 1 cut(s) 737
AspS9I GGNCC 4 cut(s) 135, 136, 399, 539
AsuHPI GGTGA 5 cut(s) 35, 88, 428, 518, 541
BaeGI GKGCMC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 247
BbvCI CCTCAGC 1 cut(s) 780
BbvI GCAGC 2 cut(s) 96, 828
BccI CCATC 4 cut(s) 288, 475, 684, 806
BciT130I CCWGG 3 cut(s) 403, 553, 605
BciVI GTATCC 1 cut(s) 323
BclI TGATCA 1 cut(s) 523
BcoDI GTCTC 1 cut(s) 643
BfaI CTAG 3 cut(s) 380, 474, 593
BfuI GTATCC 1 cut(s) 323
BisI GCNGC 2 cut(s) 85, 817
BlsI GCNGC 2 cut(s) 86, 818
Bme1390I CCNGG 3 cut(s) 403, 553, 605
BmgT120I GGNCC 4 cut(s) 135, 136, 399, 539
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 3 cut(s) 403, 553, 605
BmsI GCATC 1 cut(s) 576
BpiI GAAGAC 1 cut(s) 247
BpmI CTGGAG 2 cut(s) 65, 574
Bpu10I CCTNAGC 1 cut(s) 780
BpuEI CTTGAG 1 cut(s) 625
BsaHI GRCGYC 1 cut(s) 844
BsaI GGTCTC 1 cut(s) 643
BsaJI CCNNGG 1 cut(s) 401
BsaWI WCCGGW 1 cut(s) 634
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 748
Bse1I ACTGG 3 cut(s) 69, 322, 761
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 3 cut(s) 403, 553, 605
BseDI CCNNGG 1 cut(s) 401
BseGI GGATG 2 cut(s) 280, 814
BseLI CCNNNNNNNGG 2 cut(s) 133, 748
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 2 cut(s) 30, 771
BseNI ACTGG 3 cut(s) 69, 322, 761
BseSI GKGCMC 1 cut(s) 139
BseXI GCAGC 2 cut(s) 96, 828
BseYI CCCAGC 1 cut(s) 269
BsgI GTGCAG 1 cut(s) 859
BshFI GGCC 6 cut(s) 29, 137, 298, 400, 459, 540
BsiSI CCGG 2 cut(s) 570, 635
BslI CCNNNNNNNGG 2 cut(s) 133, 748
BsmAI GTCTC 1 cut(s) 643
BsnI GGCC 6 cut(s) 29, 137, 298, 400, 459, 540
Bso31I GGTCTC 1 cut(s) 643
Bsp120I GGGCCC 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 139
Bsp143I GATC 3 cut(s) 94, 123, 523
BspACI CCGC 2 cut(s) 128, 414
BspANI GGCC 6 cut(s) 29, 137, 298, 400, 459, 540
BspCNI CTCAG 2 cut(s) 31, 772
BspLI GGNNCC 1 cut(s) 137
BspPI GGATC 1 cut(s) 118
BspTNI GGTCTC 1 cut(s) 643
BsrBI CCGCTC 1 cut(s) 130
BsrDI GCAATG 1 cut(s) 192
BsrI ACTGG 3 cut(s) 69, 322, 761
BssECI CCNNGG 1 cut(s) 401
BssMI GATC 3 cut(s) 94, 123, 523
BssNI GRCGYC 1 cut(s) 844
Bst2UI CCWGG 3 cut(s) 403, 553, 605
Bst4CI ACNGT 2 cut(s) 663, 835
BstACI GRCGYC 1 cut(s) 844
BstC8I GCNNGC 4 cut(s) 31, 398, 461, 842
BstDEI CTNAG 5 cut(s) 39, 224, 666, 714, 780
BstF5I GGATG 2 cut(s) 280, 814
BstKTI GATC 3 cut(s) 97, 126, 526
BstMAI GTCTC 1 cut(s) 643
BstMBI GATC 3 cut(s) 94, 123, 523
BstMWI GCNNNNNNNGC 1 cut(s) 127
BstNI CCWGG 3 cut(s) 403, 553, 605
BstNSI RCATGY 1 cut(s) 569
BstSCI CCNGG 3 cut(s) 401, 551, 603
BstSLI GKGCMC 1 cut(s) 139
BstV1I GCAGC 2 cut(s) 96, 828
BstV2I GAAGAC 1 cut(s) 247
BstXI CCANNNNNNTGG 1 cut(s) 536
BsuI GTATCC 1 cut(s) 323
BsuRI GGCC 6 cut(s) 29, 137, 298, 400, 459, 540
BtsCI GGATG 2 cut(s) 280, 814
BtsIMutI CAGTG 1 cut(s) 754
Cac8I GCNNGC 4 cut(s) 31, 398, 461, 842
Cfr13I GGNCC 4 cut(s) 135, 136, 399, 539
CseI GACGC 2 cut(s) 578, 833
Csp6I GTAC 2 cut(s) 698, 725
CviAII CATG 2 cut(s) 181, 566
CviQI GTAC 2 cut(s) 698, 725
DdeI CTNAG 5 cut(s) 39, 224, 666, 714, 780
DpnI GATC 3 cut(s) 96, 125, 525
DpnII GATC 3 cut(s) 94, 123, 523
Eco147I AGGCCT 2 cut(s) 29, 459
Eco24I GRGCYC 1 cut(s) 139
Eco31I GGTCTC 1 cut(s) 643
EcoRII CCWGG 3 cut(s) 401, 551, 603
EcoT38I GRGCYC 1 cut(s) 139
FaeI CATG 2 cut(s) 184, 569
FaiI YATR 6 cut(s) 172, 182, 232, 327, 567, 708
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FatI CATG 2 cut(s) 180, 565
FauI CCCGC 1 cut(s) 135
FbaI TGATCA 1 cut(s) 523
Fnu4HI GCNGC 2 cut(s) 85, 817
FokI GGATG 2 cut(s) 287, 821
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 2 cut(s) 85, 817
FspBI CTAG 3 cut(s) 380, 474, 593
GluI GCNGC 2 cut(s) 85, 817
GsaI CCCAGC 1 cut(s) 273
GsuI CTGGAG 2 cut(s) 65, 574
HaeIII GGCC 6 cut(s) 29, 137, 298, 400, 459, 540
HapII CCGG 2 cut(s) 570, 635
HgaI GACGC 2 cut(s) 578, 833
Hin1I GRCGYC 1 cut(s) 844
Hin1II CATG 2 cut(s) 184, 569
HinfI GANTC 5 cut(s) 220, 430, 485, 600, 738
HpaII CCGG 2 cut(s) 570, 635
HphI GGTGA 5 cut(s) 35, 88, 428, 518, 541
Hpy188I TCNGA 4 cut(s) 40, 238, 657, 673
Hpy188III TCNNGA 4 cut(s) 284, 373, 593, 642
HpyAV CCTTC 4 cut(s) 196, 200, 290, 442
HpyCH4III ACNGT 2 cut(s) 663, 835
HpyCH4V TGCA 2 cut(s) 61, 840
HpyF10VI GCNNNNNNNGC 1 cut(s) 127
HpyF3I CTNAG 5 cut(s) 39, 224, 666, 714, 780
Hsp92I GRCGYC 1 cut(s) 844
Hsp92II CATG 2 cut(s) 184, 569
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 3 cut(s) 94, 123, 523
LmnI GCTCC 3 cut(s) 195, 343, 364
Lsp1109I GCAGC 2 cut(s) 96, 828
LweI GCATC 1 cut(s) 576
MaeI CTAG 3 cut(s) 380, 474, 593
MaeIII GTNAC 1 cut(s) 759
MalI GATC 3 cut(s) 96, 125, 525
MbiI CCGCTC 1 cut(s) 130
MboI GATC 3 cut(s) 94, 123, 523
MboII GAAGA 3 cut(s) 247, 587, 742
MfeI CAATTG 1 cut(s) 391
MhlI GDGCHC 1 cut(s) 139
MluCI AATT 6 cut(s) 197, 391, 408, 579, 743, 767
MmeI TCCRAC 1 cut(s) 672
MroXI GAANNNNTTC 1 cut(s) 737
MseI TTAA 6 cut(s) 113, 356, 387, 407, 423, 582
MslI CAYNNNNRTG 2 cut(s) 185, 249
MspI CCGG 2 cut(s) 570, 635
MspR9I CCNGG 3 cut(s) 403, 553, 605
MunI CAATTG 1 cut(s) 391
MvaI CCWGG 3 cut(s) 403, 553, 605
MwoI GCNNNNNNNGC 1 cut(s) 127
NdeII GATC 3 cut(s) 94, 123, 523
NlaIII CATG 2 cut(s) 184, 569
NlaIV GGNNCC 1 cut(s) 137
NspI RCATGY 1 cut(s) 569
PceI AGGCCT 2 cut(s) 29, 459
PdmI GAANNNNTTC 1 cut(s) 737
PfeI GAWTC 5 cut(s) 220, 430, 485, 600, 738
PkrI GCNGC 2 cut(s) 86, 818
Psp6I CCWGG 3 cut(s) 401, 551, 603
PspFI CCCAGC 1 cut(s) 269
PspGI CCWGG 3 cut(s) 401, 551, 603
PspN4I GGNNCC 1 cut(s) 137
PspOMI GGGCCC 1 cut(s) 135
PspPI GGNCC 4 cut(s) 135, 136, 399, 539
PsrI GAACNNNNNNTAC 2 cut(s) 744, 776
RsaI GTAC 2 cut(s) 699, 726
RsaNI GTAC 2 cut(s) 698, 725
RseI CAYNNNNRTG 2 cut(s) 185, 249
SaqAI TTAA 6 cut(s) 113, 356, 387, 407, 423, 582
SatI GCNGC 2 cut(s) 85, 817
Sau3AI GATC 3 cut(s) 94, 123, 523
Sau96I GGNCC 4 cut(s) 135, 136, 399, 539
ScrFI CCNGG 3 cut(s) 403, 553, 605
SduI GDGCHC 1 cut(s) 139
SfaNI GCATC 1 cut(s) 576
SmiMI CAYNNNNRTG 2 cut(s) 185, 249
SmlI CTYRAG 1 cut(s) 640
SmoI CTYRAG 1 cut(s) 640
Sse9I AATT 6 cut(s) 197, 391, 408, 579, 743, 767
SseBI AGGCCT 2 cut(s) 29, 459
SsiI CCGC 2 cut(s) 128, 414
SspMI CTAG 3 cut(s) 380, 474, 593
StuI AGGCCT 2 cut(s) 29, 459
StyD4I CCNGG 3 cut(s) 401, 551, 603
TaaI ACNGT 2 cut(s) 663, 835
TasI AATT 6 cut(s) 197, 391, 408, 579, 743, 767
TatI WGTACW 1 cut(s) 697
TfiI GAWTC 5 cut(s) 220, 430, 485, 600, 738
Tru1I TTAA 6 cut(s) 113, 356, 387, 407, 423, 582
Tru9I TTAA 6 cut(s) 113, 356, 387, 407, 423, 582
TscAI CASTG 1 cut(s) 761
TseI GCWGC 2 cut(s) 84, 816
TspDTI ATGAA 1 cut(s) 247
TspGWI ACGGA 2 cut(s) 171, 742
TspRI CASTG 1 cut(s) 761
XbaI TCTAGA 1 cut(s) 592
XceI RCATGY 1 cut(s) 569
XmnI GAANNNNTTC 1 cut(s) 737
XspI CTAG 3 cut(s) 380, 474, 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.