MD16G1116300.v1.1

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000168
Physical Location & Seq
Reverse (-)
8257822 .. 8258775
954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1116300.v1.1.491

Sequence Viewer

Length: 954 bp
ATGGACATCTTGAGTTGCATACTACTAGGTCTAATGGTTGCATGGATCTCAATTTATACCCTCTATTATTCACTTGGAAGAAGATCATCAGTTACCACAAGGCTCCCACCTGGACCAAACCCACTTCCCTTCATTGGCAATCTCTTAGAGCTTGGCAACAAACCCCATCTCTCTCTCACCAAGCTTTCACAACGCTACGGCCCCATAATGGCCTTGCAACTCGGCCAAATAACGACGGTGGTAATTTCTTCATCAACCTTCGCCAAAGAAGTCCTCCGAACCCACGACCAACTCTTCTGCAACCGAACCGTCCCAGATGTGCTCCGAGCTTGTCAACATTCCAAGTACCGCATGGCTTGGATACCGGTTTCACCTGCTTGGAGAAACCTTCGCAAAACATGTAACACGCAATTGTTCGCCACCAAAATTTTCGATGCCAACCAAGCCAACCGTCACCTAAAAGTGCAAGAGCTCATATCTTACGTCAATGAAAGCGCTGTAAACGGTGAGGTGGTTGATATCGGAAAGGCTGCTTTCAAAACTTCGCTCAATTTGCTGTTGCGTACAGTCTTCTCCGTGGATTTAGCGGACCCACACAGTGCGATGGCGAGAGAGTTCAAGGACTTGGTGTGGGGTATCTTTGAAGAGGCCGGGAAACCTAACTTGGTTGACTATTTTCCTATGCTCAAAAAGATCGATCCCCTGGGAATTCGACGCCGTTTGACCGGTCACTTCCGAAAGATGCTTGACCTCTTTGACCGCTTGATGATGCAACGGTTCGAGTCAAGAAAAGTGCTTGATTATATCATGACTAATGATATGTTAGATACCCTGATAAACATTAGTAAGGAGAAAAATGAGGATATGGACATCGATGAAATTCAACATCTGCTCCTGGTTAGTTGTCTACTCTCTACCTTCATTGTTCTTGATCTTGATGAACTGATTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

318

Amino Acids

36.25

Weight (kDa)

8.73

Isoelectric Point (pI)

33.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 36 - 299 7.3e-32 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61035 AT3G61035 AT3G61035 AT3G61035
fragaria_vesca FvH4_4g24041 FvH4_4g24050 FvH4_4g24050 FvH4_4g24052 FvH4_4g24053 FvH4_4g24060 FvH4_4g24060 FvH4_4g24070 FvH4_4g24080 FvH4_4g24230 FvH4_4g24640 FvH4_4g27900
malus_domestica MD06G1131200.v1.1 MD07G1012100.v1.1 MD12G1099200.v1.1 MD13G1115700.v1.1 MD13G1116100.v1.1 MD16G1115800.v1.1 MD16G1115900.v1.1 MD16G1116200.v1.1 MD16G1116300.v1.1
prunus_persica Prupe.1G229900_v2.0.a1 Prupe.1G230300_v2.0.a1 Prupe.1G230500_v2.0.a1 Prupe.1G230600_v2.0.a1 Prupe.1G230800_v2.0.a1 Prupe.1G230900_v2.0.a1 Prupe.1G231000_v2.0.a1 Prupe.1G231300_v2.0.a1 Prupe.1G231400_v2.0.a1 Prupe.6G237700_v2.0.a1
pyrus_communis pycom13g09840 pycom13g10020 pycom13g10040 pycom13g10080 pycom13g16060 pycom13g16080 pycom13g16090 pycom16g09820 pycom16g09830 pycom16g09860 pycom16g09870 pycom16g09880 pycom16g09920
rosa_chinensis RchiOBHm_Chr4g0417141 RchiOBHm_Chr4g0430071 RchiOBHm_Chr4g0430761 RchiOBHm_Chr4g0430771 RchiOBHm_Chr4g0430801 RchiOBHm_Chr4g0430821 RchiOBHm_Chr4g0430841 RchiOBHm_Chr4g0430851 RchiOBHm_Chr4g0431011 RchiOBHm_Chr4g0431021 RchiOBHm_Chr4g0431631 RchiOBHm_Chr4g0431641 RchiOBHm_Chr4g0431651 RchiOBHm_Chr4g0431661 RchiOBHm_Chr4g0431751 RchiOBHm_Chr7g0218001 RchiOBHm_Chr7g0218961 RchiOBHm_Chr7g0222351
rosa_laevigata RLG00000002068 RLG00000002315 RLG00000006893 RLG00000006900 RLG00000006902 RLG00000006934 RLG00000006946 RLG00000006947 RLG00000006949 RLG00000006951 RLG00000007006 RLG00000007007 RLG00000007928 RLG00000008331
rosa_multiflora Rmu_co8203018.1_g000001 Rmu_co8382437.1_g000001 Rmu_co8416363.1_g000001 Rmu_co8420493.1_g000001 Rmu_sc0000131.1_g000002 Rmu_sc0000289.1_g000009 Rmu_sc0001459.1_g000023 Rmu_sc0002620.1_g000010 Rmu_sc0002620.1_g000015 Rmu_sc0002620.1_g000019 Rmu_sc0003823.1_g000013 Rmu_sc0006263.1_g000004 Rmu_sc0007069.1_g000002 Rmu_sc0008769.1_g000020 Rmu_sc0009082.1_g000005 Rmu_sc0009440.1_g000005 Rmu_sc0016424.1_g000002 Rmu_sc0017974.1_g000003 Rmu_sc0023197.1_g000002 Rmu_sc0030497.1_g000002 Rmu_ssc0000150.1_g000031 Rmu_ssc0000340.1_g000006 Rmu_ssc0000487.1_g000004
rosa_roxburghii Rroxscaffold_3G00237050 Rroxscaffold_3G00240290 Rroxscaffold_4G00308920 Rroxscaffold_5G00360950 Rroxscaffold_5G00371870 Rroxscaffold_5G00371880 Rroxscaffold_5G00372470 Rroxscaffold_5G00372500 Rroxscaffold_5G00372510 Rroxscaffold_5G00372520 Rroxscaffold_5G00373090
rosa_rugosa Rorug01G0177500 Rorug03G0283700 Rorug04G0073600 Rorug04G0239800 Rorug04G0245700 Rorug04G0245800 Rorug04G0245800 Rorug04G0245900 Rorug04G0251500 Rorug04G0251600 Rorug04G0252200 Rorug07G0180200 Rorug07G0207700
rosa_samantha Rh4AG208700 Rh4AG296900 Rh4AG301700 Rh4AG301900 Rh4AG302000 Rh4AG302100 Rh4AG302200 Rh4AG304200 Rh4AG308200 Rh4AG308500 Rh4BG167800 Rh4BG205800 Rh4BG303400 Rh4BG309700 Rh4BG309800 Rh4BG310000 Rh4BG310100 Rh4BG310200 Rh4BG310300 Rh4BG315800 Rh4BG316300 Rh4CG219700 Rh4CG318900 Rh4CG326300 Rh4CG326400 Rh4CG326500 Rh4CG326600 Rh4CG331300 Rh7AG315200 Rh7AG323400 Rh7AG350100 Rh7BG314600 Rh7BG339700 Rh7CG332600 Rh7CG340200 Rh7CG340300 Rh7CG367600
rosa_wichuraiana Rw0G015620 Rw4G017690 Rw4G025740 Rw4G026220 Rw4G026240 Rw4G026260 Rw4G026720 Rw4G026730 Rw4G026830 Rw7G027400 Rw7G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 382
Acc36I ACCTGC 1 cut(s) 382
AccI GTMKAC 1 cut(s) 907
AciI CCGC 3 cut(s) 349, 587, 760
AclWI GGATC 2 cut(s) 53, 692
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 3 cut(s) 426, 708, 879
AcyI GRCGYC 1 cut(s) 715
AdeI CACNNNGTG 1 cut(s) 599
AfaI GTAC 2 cut(s) 347, 565
AfeI AGCGCT 1 cut(s) 496
AfiI CCNNNNNNNGG 2 cut(s) 134, 208
AflIII ACRYGT 1 cut(s) 398
AgeI ACCGGT 2 cut(s) 364, 725
AgsI TTSAA 4 cut(s) 538, 619, 644, 884
AjnI CCWGG 3 cut(s) 109, 702, 894
AjuI GAANNNNNNNTTGG 2 cut(s) 647, 679
AluBI AGCT 4 cut(s) 151, 184, 329, 472
AluI AGCT 4 cut(s) 151, 184, 329, 472
Alw21I GWGCWC 2 cut(s) 324, 474
AlwI GGATC 2 cut(s) 53, 692
Aor51HI AGCGCT 1 cut(s) 496
AoxI GGCC 4 cut(s) 199, 210, 223, 648
ApeKI GCWGC 1 cut(s) 530
ApoI RAATTY 3 cut(s) 426, 708, 879
AsiGI ACCGGT 2 cut(s) 364, 725
Asp700I GAANNNNTTC 1 cut(s) 945
AspLEI GCGC 1 cut(s) 497
AspS9I GGNCC 3 cut(s) 113, 200, 589
AsuC2I CCSGG 1 cut(s) 652
AsuHPI GGTGA 4 cut(s) 169, 363, 446, 518
AvaII GGWCC 2 cut(s) 113, 589
BanII GRGCYC 1 cut(s) 474
BbsI GAAGAC 1 cut(s) 562
Bbv12I GWGCWC 2 cut(s) 324, 474
BbvI GCAGC 1 cut(s) 517
BccI CCATC 2 cut(s) 174, 598
BceAI ACGGC 2 cut(s) 214, 702
BciT130I CCWGG 3 cut(s) 111, 704, 896
BciVI GTATCC 1 cut(s) 354
BcnI CCSGG 1 cut(s) 652
BfaI CTAG 1 cut(s) 26
BfoI RGCGCY 1 cut(s) 498
BfuAI ACCTGC 1 cut(s) 382
BfuI GTATCC 1 cut(s) 354
BisI GCNGC 1 cut(s) 531
BlsI GCNGC 1 cut(s) 532
Bme1390I CCNGG 4 cut(s) 111, 652, 704, 896
Bme18I GGWCC 2 cut(s) 113, 589
BmgT120I GGNCC 3 cut(s) 113, 200, 589
BmiI GGNNCC 3 cut(s) 104, 202, 591
BmrFI CCNGG 4 cut(s) 111, 652, 704, 896
BmsI GCATC 3 cut(s) 424, 732, 759
BpiI GAAGAC 1 cut(s) 562
BpuEI CTTGAG 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 652
Bsa29I ATCGAT 2 cut(s) 696, 873
BsaHI GRCGYC 1 cut(s) 715
BsaJI CCNNGG 3 cut(s) 576, 702, 703
BsaWI WCCGGW 2 cut(s) 364, 725
BsaXI ACNNNNNCTCC 2 cut(s) 876, 906
Bsc4I CCNNNNNNNGG 2 cut(s) 134, 208
Bse118I RCCGGY 2 cut(s) 364, 725
BseBI CCWGG 3 cut(s) 111, 704, 896
BseCI ATCGAT 2 cut(s) 696, 873
BseDI CCNNGG 3 cut(s) 576, 702, 703
BseLI CCNNNNNNNGG 2 cut(s) 134, 208
BseXI GCAGC 1 cut(s) 517
BshFI GGCC 4 cut(s) 201, 212, 225, 650
BshTI ACCGGT 2 cut(s) 364, 725
BshVI ATCGAT 2 cut(s) 696, 873
BsiHKAI GWGCWC 2 cut(s) 324, 474
BsiSI CCGG 3 cut(s) 365, 651, 726
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 2 cut(s) 134, 208
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 4 cut(s) 201, 212, 225, 650
Bsp1286I GDGCHC 2 cut(s) 324, 474
Bsp143I GATC 5 cut(s) 45, 83, 693, 697, 931
BspACI CCGC 3 cut(s) 349, 587, 760
BspANI GGCC 4 cut(s) 201, 212, 225, 650
BspDI ATCGAT 2 cut(s) 696, 873
BspHI TCATGA 1 cut(s) 807
BspLI GGNNCC 3 cut(s) 104, 202, 591
BspMI ACCTGC 1 cut(s) 382
BspPI GGATC 2 cut(s) 53, 692
BsrFI RCCGGY 2 cut(s) 364, 725
BssAI RCCGGY 2 cut(s) 364, 725
BssECI CCNNGG 3 cut(s) 576, 702, 703
BssMI GATC 5 cut(s) 45, 83, 693, 697, 931
BssNI GRCGYC 1 cut(s) 715
Bst2UI CCWGG 3 cut(s) 111, 704, 896
Bst4CI ACNGT 7 cut(s) 238, 310, 452, 506, 568, 599, 777
Bst6I CTCTTC 2 cut(s) 299, 639
BstACI GRCGYC 1 cut(s) 715
BstDEI CTNAG 1 cut(s) 145
BstDSI CCRYGG 1 cut(s) 576
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 1 cut(s) 497
BstKTI GATC 5 cut(s) 48, 86, 696, 700, 934
BstMBI GATC 5 cut(s) 45, 83, 693, 697, 931
BstMWI GCNNNNNNNGC 2 cut(s) 443, 553
BstNI CCWGG 3 cut(s) 111, 704, 896
BstNSI RCATGY 1 cut(s) 402
BstSCI CCNGG 4 cut(s) 109, 650, 702, 894
BstV1I GCAGC 1 cut(s) 517
BstV2I GAAGAC 1 cut(s) 562
BstX2I RGATCY 1 cut(s) 45
BstYI RGATCY 1 cut(s) 45
Bsu15I ATCGAT 2 cut(s) 696, 873
BsuI GTATCC 1 cut(s) 354
BsuRI GGCC 4 cut(s) 201, 212, 225, 650
BsuTUI ATCGAT 2 cut(s) 696, 873
BtgI CCRYGG 1 cut(s) 576
BtgZI GCGATG 1 cut(s) 617
BtsIMutI CAGTG 1 cut(s) 604
BveI ACCTGC 1 cut(s) 382
CciI TCATGA 1 cut(s) 807
CfoI GCGC 1 cut(s) 497
Cfr10I RCCGGY 2 cut(s) 364, 725
Cfr13I GGNCC 3 cut(s) 113, 200, 589
ClaI ATCGAT 2 cut(s) 696, 873
CseI GACGC 1 cut(s) 723
Csp6I GTAC 2 cut(s) 346, 564
CspAI ACCGGT 2 cut(s) 364, 725
CviAII CATG 4 cut(s) 42, 352, 399, 808
CviQI GTAC 2 cut(s) 346, 564
DdeI CTNAG 1 cut(s) 145
DpnI GATC 5 cut(s) 47, 85, 695, 699, 933
DpnII GATC 5 cut(s) 45, 83, 693, 697, 931
DraIII CACNNNGTG 1 cut(s) 599
EaeI YGGCCR 1 cut(s) 223
Eam1104I CTCTTC 2 cut(s) 299, 639
EarI CTCTTC 2 cut(s) 299, 639
Ecl136II GAGCTC 1 cut(s) 472
Eco24I GRGCYC 1 cut(s) 474
Eco32I GATATC 1 cut(s) 520
Eco47I GGWCC 2 cut(s) 113, 589
Eco47III AGCGCT 1 cut(s) 496
Eco53kI GAGCTC 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 472
EcoRI GAATTC 1 cut(s) 708
EcoRII CCWGG 3 cut(s) 109, 702, 894
EcoRV GATATC 1 cut(s) 520
EcoT38I GRGCYC 1 cut(s) 474
FaeI CATG 4 cut(s) 45, 355, 402, 811
FaqI GGGAC 1 cut(s) 296
FatI CATG 4 cut(s) 41, 351, 398, 807
FblI GTMKAC 1 cut(s) 907
Fnu4HI GCNGC 1 cut(s) 531
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 1 cut(s) 531
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 496
GluI GCNGC 1 cut(s) 531
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 4 cut(s) 201, 212, 225, 650
HapII CCGG 3 cut(s) 365, 651, 726
HgaI GACGC 1 cut(s) 723
HhaI GCGC 1 cut(s) 497
Hin1I GRCGYC 1 cut(s) 715
Hin1II CATG 4 cut(s) 45, 355, 402, 811
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HincII GTYRAC 2 cut(s) 335, 670
HindII GTYRAC 2 cut(s) 335, 670
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 2 cut(s) 782, 946
HpaII CCGG 3 cut(s) 365, 651, 726
HphI GGTGA 4 cut(s) 169, 363, 446, 518
Hpy166II GTNNAC 4 cut(s) 335, 502, 670, 908
Hpy188I TCNGA 4 cut(s) 278, 326, 524, 737
Hpy188III TCNNGA 5 cut(s) 10, 786, 808, 929, 935
Hpy8I GTNNAC 4 cut(s) 335, 502, 670, 908
Hpy99I CGWCG 2 cut(s) 238, 717
HpyAV CCTTC 4 cut(s) 139, 268, 398, 928
HpyCH4III ACNGT 7 cut(s) 238, 310, 452, 506, 568, 599, 777
HpyCH4IV ACGT 1 cut(s) 483
HpyCH4V TGCA 6 cut(s) 18, 41, 217, 300, 466, 772
HpyF10VI GCNNNNNNNGC 2 cut(s) 443, 553
HpyF3I CTNAG 1 cut(s) 145
HpySE526I ACGT 1 cut(s) 483
Hsp92I GRCGYC 1 cut(s) 715
Hsp92II CATG 4 cut(s) 45, 355, 402, 811
HspAI GCGC 1 cut(s) 495
Kzo9I GATC 5 cut(s) 45, 83, 693, 697, 931
LmnI GCTCC 3 cut(s) 108, 327, 897
Lsp1109I GCAGC 1 cut(s) 517
LweI GCATC 3 cut(s) 424, 732, 759
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 483
MaeIII GTNAC 4 cut(s) 91, 401, 452, 728
MalI GATC 5 cut(s) 47, 85, 695, 699, 933
MboI GATC 5 cut(s) 45, 83, 693, 697, 931
MboII GAAGA 6 cut(s) 90, 93, 240, 286, 562, 656
MfeI CAATTG 1 cut(s) 410
MflI RGATCY 1 cut(s) 45
MhlI GDGCHC 2 cut(s) 324, 474
MluCI AATT 7 cut(s) 51, 243, 410, 426, 550, 708, 879
MlyI GAGTC 1 cut(s) 791
MnlI CCTC 6 cut(s) 71, 284, 502, 640, 761, 853
MroXI GAANNNNTTC 1 cut(s) 945
MspI CCGG 3 cut(s) 365, 651, 726
MspR9I CCNGG 4 cut(s) 111, 652, 704, 896
MunI CAATTG 1 cut(s) 410
MvaI CCWGG 3 cut(s) 111, 704, 896
MwoI GCNNNNNNNGC 2 cut(s) 443, 553
NciI CCSGG 1 cut(s) 652
NdeII GATC 5 cut(s) 45, 83, 693, 697, 931
NlaIII CATG 4 cut(s) 45, 355, 402, 811
NlaIV GGNNCC 3 cut(s) 104, 202, 591
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 2 cut(s) 452, 728
NspI RCATGY 1 cut(s) 402
PagI TCATGA 1 cut(s) 807
PaqCI CACCTGC 1 cut(s) 382
PasI CCCWGGG 1 cut(s) 703
PciI ACATGT 1 cut(s) 398
PdmI GAANNNNTTC 1 cut(s) 945
PfeI GAWTC 1 cut(s) 946
PinAI ACCGGT 2 cut(s) 364, 725
PkrI GCNGC 1 cut(s) 532
PleI GAGTC 1 cut(s) 790
PpsI GAGTC 1 cut(s) 790
PscI ACATGT 1 cut(s) 398
Psp124BI GAGCTC 1 cut(s) 474
Psp6I CCWGG 3 cut(s) 109, 702, 894
PspGI CCWGG 3 cut(s) 109, 702, 894
PspN4I GGNNCC 3 cut(s) 104, 202, 591
PspPI GGNCC 3 cut(s) 113, 200, 589
PsuI RGATCY 1 cut(s) 45
RsaI GTAC 2 cut(s) 347, 565
RsaNI GTAC 2 cut(s) 346, 564
SacI GAGCTC 1 cut(s) 474
SatI GCNGC 1 cut(s) 531
Sau3AI GATC 5 cut(s) 45, 83, 693, 697, 931
Sau96I GGNCC 3 cut(s) 113, 200, 589
SchI GAGTC 1 cut(s) 791
ScrFI CCNGG 4 cut(s) 111, 652, 704, 896
SduI GDGCHC 2 cut(s) 324, 474
SfaNI GCATC 3 cut(s) 424, 732, 759
SinI GGWCC 2 cut(s) 113, 589
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 7 cut(s) 51, 243, 410, 426, 550, 708, 879
SsiI CCGC 3 cut(s) 349, 587, 760
SspMI CTAG 1 cut(s) 26
SstI GAGCTC 1 cut(s) 474
StyD4I CCNGG 4 cut(s) 109, 650, 702, 894
TaaI ACNGT 7 cut(s) 238, 310, 452, 506, 568, 599, 777
TaiI ACGT 1 cut(s) 486
TaqI TCGA 5 cut(s) 432, 696, 712, 780, 873
TasI AATT 7 cut(s) 51, 243, 410, 426, 550, 708, 879
TfiI GAWTC 1 cut(s) 946
TscAI CASTG 1 cut(s) 604
TseFI GTSAC 2 cut(s) 452, 728
TseI GCWGC 1 cut(s) 530
Tsp45I GTSAC 2 cut(s) 452, 728
TspDTI ATGAA 6 cut(s) 121, 240, 504, 891, 910, 954
TspGWI ACGGA 1 cut(s) 565
TspRI CASTG 1 cut(s) 604
VpaK11BI GGWCC 2 cut(s) 113, 589
XapI RAATTY 3 cut(s) 426, 708, 879
XceI RCATGY 1 cut(s) 402
XcmI CCANNNNNNNNNTGG 1 cut(s) 349
XmiI GTMKAC 1 cut(s) 907
XmnI GAANNNNTTC 1 cut(s) 945
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.