Rh4AG304200

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000168
Physical Location & Seq
Reverse (-)
61618050 .. 61631514
13465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG304200.1

Sequence Viewer

Length: 1026 bp
ATGTTTCAAGAGGTGGGGAAACCAAACTTGGCGGATTATAATTTTCCTTTCCTTAGGAAGCTTGATCCACAAGGCATAAGGCGGCGCTTGACCCATTACTTCCAGAAAACGTTGGACATCTTTGATCGACTGATCCATCAAAGGTTGGAATCAAAAAAACGAGACGGTTATATCTCAACCAATGATATGTTGTATACACTTCTCAACATCAGTGAAGAGAAGACGGAGAATATAGATATGCCCGAAACTCAACATTTATTTCTGGATCTATTTTCTGCTGGCACAGACACAAGTTCAGCCACAATGGAATGGGCAATGGCTGAGCTACTACGCAACCCGAAAACCCTTTCGAAAGCTCAAGCAGAACTCGAGCAAGTGATTGGAAAAGGGAAACTAGTTGAGGAATCAGACATTGCACGACTCCCTTACTTACAAGCAATAATCAAAGAAACATTTCGAATTCACCCAACAGTTCCATTTTTACTCCCCCGGAAAGCAGAAACAAACATAGAACTTGGCGGGTATATAATCCCAAAGGGTGCACGAGTTCTAATCAATTTTTGGGCCATAAGCAGAGACCCCATCACTTGGGACAACCCAAACTTGTTTATGCCTGAGAGGTTCCTGGGATTAGACAACCAAATTGATGTTACGGGGAAAAACTTTGAGCTTATTCCGTTTGGTGGTGGGAGGAGAATATGTCCCGGACTTCCATTGGCAGTAAGAATGTTGCACTTGATGTTGGGCTCACTAATTAACTGCTTTGATTGGAAGCTTGAAGATGGAGTTGTACCAGAGACTATGAACATGGAAGATAAGTTTGGCCTCACCTTACAAATGGCTCAGCCTCTGAGAGCTGTGCCTAAGAATCTGCAATTACTTATGTCGTTTACAATGTGGCCCGAGAATAATAACACAGAGTCACAGACTGATATTGACATGGACCTAATCTATTATCTACGTACTGATTCTGATTGTACTAGAAAATCAAAATCTGGTAGGGAAGGAAAAGCCGTGATCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

341

Amino Acids

39.19

Weight (kDa)

5.86

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 5 - 276 6.4e-72 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61035 AT3G61035 AT3G61035 AT3G61035
fragaria_vesca FvH4_4g24041 FvH4_4g24050 FvH4_4g24050 FvH4_4g24052 FvH4_4g24053 FvH4_4g24060 FvH4_4g24060 FvH4_4g24070 FvH4_4g24080 FvH4_4g24230 FvH4_4g24640 FvH4_4g27900
malus_domestica MD06G1131200.v1.1 MD07G1012100.v1.1 MD12G1099200.v1.1 MD13G1115700.v1.1 MD13G1116100.v1.1 MD16G1115800.v1.1 MD16G1115900.v1.1 MD16G1116200.v1.1 MD16G1116300.v1.1
prunus_persica Prupe.1G229900_v2.0.a1 Prupe.1G230300_v2.0.a1 Prupe.1G230500_v2.0.a1 Prupe.1G230600_v2.0.a1 Prupe.1G230800_v2.0.a1 Prupe.1G230900_v2.0.a1 Prupe.1G231000_v2.0.a1 Prupe.1G231300_v2.0.a1 Prupe.1G231400_v2.0.a1 Prupe.6G237700_v2.0.a1
pyrus_communis pycom13g09840 pycom13g10020 pycom13g10040 pycom13g10080 pycom13g16060 pycom13g16080 pycom13g16090 pycom16g09820 pycom16g09830 pycom16g09860 pycom16g09870 pycom16g09880 pycom16g09920
rosa_chinensis RchiOBHm_Chr4g0417141 RchiOBHm_Chr4g0430071 RchiOBHm_Chr4g0430761 RchiOBHm_Chr4g0430771 RchiOBHm_Chr4g0430801 RchiOBHm_Chr4g0430821 RchiOBHm_Chr4g0430841 RchiOBHm_Chr4g0430851 RchiOBHm_Chr4g0431011 RchiOBHm_Chr4g0431021 RchiOBHm_Chr4g0431631 RchiOBHm_Chr4g0431641 RchiOBHm_Chr4g0431651 RchiOBHm_Chr4g0431661 RchiOBHm_Chr4g0431751 RchiOBHm_Chr7g0218001 RchiOBHm_Chr7g0218961 RchiOBHm_Chr7g0222351
rosa_laevigata RLG00000002068 RLG00000002315 RLG00000006893 RLG00000006900 RLG00000006902 RLG00000006934 RLG00000006946 RLG00000006947 RLG00000006949 RLG00000006951 RLG00000007006 RLG00000007007 RLG00000007928 RLG00000008331
rosa_multiflora Rmu_co8203018.1_g000001 Rmu_co8382437.1_g000001 Rmu_co8416363.1_g000001 Rmu_co8420493.1_g000001 Rmu_sc0000131.1_g000002 Rmu_sc0000289.1_g000009 Rmu_sc0001459.1_g000023 Rmu_sc0002620.1_g000010 Rmu_sc0002620.1_g000015 Rmu_sc0002620.1_g000019 Rmu_sc0003823.1_g000013 Rmu_sc0006263.1_g000004 Rmu_sc0007069.1_g000002 Rmu_sc0008769.1_g000020 Rmu_sc0009082.1_g000005 Rmu_sc0009440.1_g000005 Rmu_sc0016424.1_g000002 Rmu_sc0017974.1_g000003 Rmu_sc0023197.1_g000002 Rmu_sc0030497.1_g000002 Rmu_ssc0000150.1_g000031 Rmu_ssc0000340.1_g000006 Rmu_ssc0000487.1_g000004
rosa_roxburghii Rroxscaffold_3G00237050 Rroxscaffold_3G00240290 Rroxscaffold_4G00308920 Rroxscaffold_5G00360950 Rroxscaffold_5G00371870 Rroxscaffold_5G00371880 Rroxscaffold_5G00372470 Rroxscaffold_5G00372500 Rroxscaffold_5G00372510 Rroxscaffold_5G00372520 Rroxscaffold_5G00373090
rosa_rugosa Rorug01G0177500 Rorug03G0283700 Rorug04G0073600 Rorug04G0239800 Rorug04G0245700 Rorug04G0245800 Rorug04G0245800 Rorug04G0245900 Rorug04G0251500 Rorug04G0251600 Rorug04G0252200 Rorug07G0180200 Rorug07G0207700
rosa_samantha Rh4AG208700 Rh4AG296900 Rh4AG301700 Rh4AG301900 Rh4AG302000 Rh4AG302100 Rh4AG302200 Rh4AG304200 Rh4AG308200 Rh4AG308500 Rh4BG167800 Rh4BG205800 Rh4BG303400 Rh4BG309700 Rh4BG309800 Rh4BG310000 Rh4BG310100 Rh4BG310200 Rh4BG310300 Rh4BG315800 Rh4BG316300 Rh4CG219700 Rh4CG318900 Rh4CG326300 Rh4CG326400 Rh4CG326500 Rh4CG326600 Rh4CG331300 Rh7AG315200 Rh7AG323400 Rh7AG350100 Rh7BG314600 Rh7BG339700 Rh7CG332600 Rh7CG340200 Rh7CG340300 Rh7CG367600
rosa_wichuraiana Rw0G015620 Rw4G017690 Rw4G025740 Rw4G026220 Rw4G026240 Rw4G026260 Rw4G026720 Rw4G026730 Rw4G026830 Rw7G027400 Rw7G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 39
AccB7I CCANNNNNTGG 1 cut(s) 588
AccI GTMKAC 1 cut(s) 194
AciI CCGC 3 cut(s) 32, 82, 519
AclI AACGTT 1 cut(s) 110
AclWI GGATC 3 cut(s) 59, 127, 273
AcsI RAATTY 1 cut(s) 459
AfaI GTAC 3 cut(s) 792, 964, 979
AfiI CCNNNNNNNGG 2 cut(s) 588, 683
AgsI TTSAA 2 cut(s) 8, 779
AhlI ACTAGT 1 cut(s) 394
AjnI CCWGG 1 cut(s) 624
AjuI GAANNNNNNNTTGG 4 cut(s) 11, 43, 804, 836
AluBI AGCT 6 cut(s) 61, 325, 356, 670, 775, 857
AluI AGCT 6 cut(s) 61, 325, 356, 670, 775, 857
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 3 cut(s) 156, 570, 791
Alw44I GTGCAC 1 cut(s) 540
AlwI GGATC 3 cut(s) 59, 127, 273
AlwNI CAGNNNCTG 1 cut(s) 850
Ama87I CYCGRG 2 cut(s) 368, 902
AoxI GGCC 3 cut(s) 564, 823, 899
ApaLI GTGCAC 1 cut(s) 540
ApoI RAATTY 1 cut(s) 459
Asp700I GAANNNNTTC 1 cut(s) 453
AspLEI GCGC 1 cut(s) 87
AspS9I GGNCC 3 cut(s) 564, 900, 943
AsuC2I CCSGG 2 cut(s) 490, 705
AsuHPI GGTGA 2 cut(s) 455, 820
AsuII TTCGAA 2 cut(s) 350, 457
AvaI CYCGRG 2 cut(s) 368, 902
AvaII GGWCC 1 cut(s) 943
AxyI CCTNAGG 1 cut(s) 53
BaeGI GKGCMC 1 cut(s) 544
BanII GRGCYC 1 cut(s) 749
BauI CACGAG 1 cut(s) 543
BbsI GAAGAC 1 cut(s) 227
Bbv12I GWGCWC 1 cut(s) 544
BccI CCATC 3 cut(s) 144, 590, 776
BceAI ACGGC 1 cut(s) 998
BciT130I CCWGG 1 cut(s) 626
BclI TGATCA 1 cut(s) 1017
BcnI CCSGG 2 cut(s) 490, 705
BcoDI GTCTC 3 cut(s) 156, 570, 791
BcuI ACTAGT 1 cut(s) 394
BfaI CTAG 3 cut(s) 395, 981, 1024
BfoI RGCGCY 1 cut(s) 88
BisI GCNGC 1 cut(s) 83
BlpI GCTNAGC 2 cut(s) 321, 843
BlsI GCNGC 1 cut(s) 84
Bme1390I CCNGG 3 cut(s) 490, 626, 705
Bme18I GGWCC 1 cut(s) 943
BmeT110I CYCGRG 2 cut(s) 368, 902
BmgT120I GGNCC 3 cut(s) 564, 900, 943
BmiI GGNNCC 1 cut(s) 623
BmrFI CCNGG 3 cut(s) 490, 626, 705
BpiI GAAGAC 1 cut(s) 227
Bpu1102I GCTNAGC 2 cut(s) 321, 843
Bpu14I TTCGAA 2 cut(s) 350, 457
BpuEI CTTGAG 1 cut(s) 342
BpuMI CCSGG 2 cut(s) 490, 705
BsaAI YACGTR 1 cut(s) 962
BsaI GGTCTC 1 cut(s) 570
BsaJI CCNNGG 2 cut(s) 488, 625
BsaXI ACNNNNNCTCC 2 cut(s) 685, 715
Bsc4I CCNNNNNNNGG 2 cut(s) 588, 683
Bse21I CCTNAGG 1 cut(s) 53
Bse3DI GCAATG 2 cut(s) 321, 411
BseBI CCWGG 1 cut(s) 626
BseDI CCNNGG 2 cut(s) 488, 625
BseLI CCNNNNNNNGG 2 cut(s) 588, 683
BseMI GCAATG 2 cut(s) 321, 411
BseMII CTCAG 4 cut(s) 312, 606, 842, 857
BseRI GAGGAG 1 cut(s) 706
BseSI GKGCMC 1 cut(s) 544
BshFI GGCC 3 cut(s) 566, 825, 901
BsiHKAI GWGCWC 1 cut(s) 544
BsiHKCI CYCGRG 2 cut(s) 368, 902
BsiSI CCGG 2 cut(s) 490, 705
BslFI GGGAC 2 cut(s) 605, 687
BslI CCNNNNNNNGG 2 cut(s) 588, 683
BsmAI GTCTC 3 cut(s) 156, 570, 791
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 2 cut(s) 605, 687
BsnI GGCC 3 cut(s) 566, 825, 901
Bso31I GGTCTC 1 cut(s) 570
BsoBI CYCGRG 2 cut(s) 368, 902
Bsp119I TTCGAA 2 cut(s) 350, 457
Bsp1286I GDGCHC 2 cut(s) 544, 749
Bsp143I GATC 5 cut(s) 64, 124, 132, 265, 1017
Bsp1720I GCTNAGC 2 cut(s) 321, 843
BspACI CCGC 3 cut(s) 32, 82, 519
BspANI GGCC 3 cut(s) 566, 825, 901
BspCNI CTCAG 4 cut(s) 313, 607, 843, 856
BspLI GGNNCC 1 cut(s) 623
BspPI GGATC 3 cut(s) 59, 127, 273
BspT104I TTCGAA 2 cut(s) 350, 457
BspTNI GGTCTC 1 cut(s) 570
BsrDI GCAATG 2 cut(s) 321, 411
BssECI CCNNGG 2 cut(s) 488, 625
BssMI GATC 5 cut(s) 64, 124, 132, 265, 1017
BssNAI GTATAC 1 cut(s) 195
BssSI CACGAG 1 cut(s) 543
Bst1107I GTATAC 1 cut(s) 195
Bst2BI CACGAG 1 cut(s) 543
Bst2UI CCWGG 1 cut(s) 626
Bst4CI ACNGT 2 cut(s) 167, 472
Bst6I CTCTTC 1 cut(s) 210
BstBAI YACGTR 1 cut(s) 962
BstBI TTCGAA 2 cut(s) 350, 457
BstC8I GCNNGC 1 cut(s) 280
BstDEI CTNAG 6 cut(s) 53, 321, 615, 843, 851, 864
BstH2I RGCGCY 1 cut(s) 88
BstHHI GCGC 1 cut(s) 87
BstKTI GATC 5 cut(s) 67, 127, 135, 268, 1020
BstMAI GTCTC 3 cut(s) 156, 570, 791
BstMBI GATC 5 cut(s) 64, 124, 132, 265, 1017
BstNI CCWGG 1 cut(s) 626
BstSCI CCNGG 3 cut(s) 488, 624, 703
BstSLI GKGCMC 1 cut(s) 544
BstSNI TACGTA 1 cut(s) 962
BstV2I GAAGAC 1 cut(s) 227
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BstZ17I GTATAC 1 cut(s) 195
Bsu36I CCTNAGG 1 cut(s) 53
BsuRI GGCC 3 cut(s) 566, 825, 901
BtsIMutI CAGTG 1 cut(s) 217
Cac8I GCNNGC 1 cut(s) 280
CaiI CAGNNNCTG 1 cut(s) 850
CfoI GCGC 1 cut(s) 87
Cfr13I GGNCC 3 cut(s) 564, 900, 943
Csp6I GTAC 3 cut(s) 791, 963, 978
CviAII CATG 2 cut(s) 808, 940
CviQI GTAC 3 cut(s) 791, 963, 978
DdeI CTNAG 6 cut(s) 53, 321, 615, 843, 851, 864
DpnI GATC 5 cut(s) 66, 126, 134, 267, 1019
DpnII GATC 5 cut(s) 64, 124, 132, 265, 1017
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
EciI GGCGGA 1 cut(s) 47
Eco105I TACGTA 1 cut(s) 962
Eco24I GRGCYC 1 cut(s) 749
Eco31I GGTCTC 1 cut(s) 570
Eco47I GGWCC 1 cut(s) 943
Eco81I CCTNAGG 1 cut(s) 53
Eco88I CYCGRG 2 cut(s) 368, 902
EcoRI GAATTC 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 624
EcoT38I GRGCYC 1 cut(s) 749
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 2 cut(s) 811, 943
FaqI GGGAC 2 cut(s) 605, 687
FatI CATG 2 cut(s) 807, 939
FauI CCCGC 1 cut(s) 512
FbaI TGATCA 1 cut(s) 1017
FblI GTMKAC 1 cut(s) 194
Fnu4HI GCNGC 1 cut(s) 83
FriOI GRGCYC 1 cut(s) 749
Fsp4HI GCNGC 1 cut(s) 83
FspBI CTAG 3 cut(s) 395, 981, 1024
GlaI GCGC 1 cut(s) 86
GluI GCNGC 1 cut(s) 83
HaeII RGCGCY 1 cut(s) 88
HaeIII GGCC 3 cut(s) 566, 825, 901
HapII CCGG 2 cut(s) 490, 705
HhaI GCGC 1 cut(s) 87
Hin1II CATG 2 cut(s) 811, 943
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HindIII AAGCTT 2 cut(s) 59, 773
HinfI GANTC 6 cut(s) 149, 404, 420, 868, 920, 968
HpaII CCGG 2 cut(s) 490, 705
HphI GGTGA 2 cut(s) 455, 820
Hpy166II GTNNAC 3 cut(s) 195, 542, 891
Hpy188I TCNGA 3 cut(s) 409, 852, 973
Hpy188III TCNNGA 3 cut(s) 8, 103, 263
Hpy8I GTNNAC 3 cut(s) 195, 542, 891
HpyAV CCTTC 1 cut(s) 998
HpyCH4III ACNGT 2 cut(s) 167, 472
HpyCH4IV ACGT 2 cut(s) 110, 961
HpyCH4V TGCA 4 cut(s) 416, 542, 733, 874
HpyF3I CTNAG 6 cut(s) 53, 321, 615, 843, 851, 864
HpySE526I ACGT 2 cut(s) 110, 961
Hsp92II CATG 2 cut(s) 811, 943
HspAI GCGC 1 cut(s) 85
Ksp22I TGATCA 1 cut(s) 1017
Kzo9I GATC 5 cut(s) 64, 124, 132, 265, 1017
MaeI CTAG 3 cut(s) 395, 981, 1024
MaeII ACGT 2 cut(s) 110, 961
MaeIII GTNAC 2 cut(s) 649, 921
MalI GATC 5 cut(s) 66, 126, 134, 267, 1019
MboI GATC 5 cut(s) 64, 124, 132, 265, 1017
MboII GAAGA 4 cut(s) 227, 232, 791, 824
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 2 cut(s) 544, 749
MluCI AATT 6 cut(s) 40, 459, 556, 642, 753, 875
MlyI GAGTC 2 cut(s) 414, 929
MmeI TCCRAC 2 cut(s) 93, 126
MnlI CCTC 6 cut(s) 4, 394, 612, 684, 836, 858
MroXI GAANNNNTTC 1 cut(s) 453
MseI TTAA 1 cut(s) 756
MspI CCGG 2 cut(s) 490, 705
MspR9I CCNGG 3 cut(s) 490, 626, 705
MvaI CCWGG 1 cut(s) 626
NciI CCSGG 2 cut(s) 490, 705
NdeII GATC 5 cut(s) 64, 124, 132, 265, 1017
NlaIII CATG 2 cut(s) 811, 943
NlaIV GGNNCC 1 cut(s) 623
NmuCI GTSAC 1 cut(s) 921
NspV TTCGAA 2 cut(s) 350, 457
PaeR7I CTCGAG 1 cut(s) 368
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 4 cut(s) 149, 404, 868, 968
PflMI CCANNNNNTGG 1 cut(s) 588
PfoI TCCNGGA 1 cut(s) 703
PkrI GCNGC 1 cut(s) 84
PleI GAGTC 2 cut(s) 414, 928
PpsI GAGTC 2 cut(s) 414, 928
Ppu21I YACGTR 1 cut(s) 962
PsiI TTATAA 1 cut(s) 39
Psp1406I AACGTT 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 624
PspGI CCWGG 1 cut(s) 624
PspN4I GGNNCC 1 cut(s) 623
PspPI GGNCC 3 cut(s) 564, 900, 943
PspXI VCTCGAGB 1 cut(s) 368
PstNI CAGNNNCTG 1 cut(s) 850
PsuI RGATCY 1 cut(s) 265
RsaI GTAC 3 cut(s) 792, 964, 979
RsaNI GTAC 3 cut(s) 791, 963, 978
SaqAI TTAA 1 cut(s) 756
SatI GCNGC 1 cut(s) 83
Sau3AI GATC 5 cut(s) 64, 124, 132, 265, 1017
Sau96I GGNCC 3 cut(s) 564, 900, 943
SchI GAGTC 2 cut(s) 414, 929
ScrFI CCNGG 3 cut(s) 490, 626, 705
SduI GDGCHC 2 cut(s) 544, 749
Sfr274I CTCGAG 1 cut(s) 368
SfuI TTCGAA 2 cut(s) 350, 457
SinI GGWCC 1 cut(s) 943
SlaI CTCGAG 1 cut(s) 368
SmlI CTYRAG 2 cut(s) 357, 368
SmoI CTYRAG 2 cut(s) 357, 368
SnaBI TACGTA 1 cut(s) 962
SpeI ACTAGT 1 cut(s) 394
Sse9I AATT 6 cut(s) 40, 459, 556, 642, 753, 875
SsiI CCGC 3 cut(s) 32, 82, 519
SspMI CTAG 3 cut(s) 395, 981, 1024
StyD4I CCNGG 3 cut(s) 488, 624, 703
TaaI ACNGT 2 cut(s) 167, 472
TaiI ACGT 2 cut(s) 113, 964
TaqI TCGA 4 cut(s) 127, 350, 369, 457
TasI AATT 6 cut(s) 40, 459, 556, 642, 753, 875
TatI WGTACW 1 cut(s) 977
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 4 cut(s) 149, 404, 868, 968
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 1 cut(s) 921
Tsp45I GTSAC 1 cut(s) 921
TspDTI ATGAA 1 cut(s) 818
TspGWI ACGGA 2 cut(s) 239, 666
TspRI CASTG 1 cut(s) 217
Van91I CCANNNNNTGG 1 cut(s) 588
VneI GTGCAC 1 cut(s) 540
VpaK11BI GGWCC 1 cut(s) 943
XapI RAATTY 1 cut(s) 459
XhoI CTCGAG 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 194
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 3 cut(s) 395, 981, 1024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.