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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 1 cut(s) | 60 |
| AgsI | TTSAA | 1 cut(s) | 93 |
| Alw21I | GWGCWC | 1 cut(s) | 109 |
| Bbv12I | GWGCWC | 1 cut(s) | 109 |
| BsaBI | GATNNNNATC | 1 cut(s) | 83 |
| Bse8I | GATNNNNATC | 1 cut(s) | 83 |
| BseJI | GATNNNNATC | 1 cut(s) | 83 |
| BsiHKAI | GWGCWC | 1 cut(s) | 109 |
| Bsp1286I | GDGCHC | 1 cut(s) | 109 |
| Bst4CI | ACNGT | 1 cut(s) | 63 |
| Bst6I | CTCTTC | 1 cut(s) | 111 |
| Csp6I | GTAC | 1 cut(s) | 59 |
| CviQI | GTAC | 1 cut(s) | 59 |
| Eam1104I | CTCTTC | 1 cut(s) | 111 |
| EarI | CTCTTC | 1 cut(s) | 111 |
| FaiI | YATR | 1 cut(s) | 48 |
| Hpy188I | TCNGA | 2 cut(s) | 17, 53 |
| Hpy188III | TCNNGA | 1 cut(s) | 110 |
| HpyCH4III | ACNGT | 1 cut(s) | 63 |
| HpyCH4V | TGCA | 1 cut(s) | 9 |
| LmnI | GCTCC | 1 cut(s) | 112 |
| LpnPI | CCDG | 2 cut(s) | 46, 123 |
| MboII | GAAGA | 2 cut(s) | 126, 128 |
| MhlI | GDGCHC | 1 cut(s) | 109 |
| MnlI | CCTC | 2 cut(s) | 11, 112 |
| MseI | TTAA | 1 cut(s) | 163 |
| RsaI | GTAC | 1 cut(s) | 60 |
| RsaNI | GTAC | 1 cut(s) | 59 |
| SaqAI | TTAA | 1 cut(s) | 163 |
| SduI | GDGCHC | 1 cut(s) | 109 |
| SetI | ASST | 3 cut(s) | 22, 100, 123 |
| SgeI | CNNG | 3 cut(s) | 45, 122, 150 |
| TaaI | ACNGT | 1 cut(s) | 63 |
| TaqI | TCGA | 1 cut(s) | 40 |
| Tru1I | TTAA | 1 cut(s) | 163 |
| Tru9I | TTAA | 1 cut(s) | 163 |
| TspDTI | ATGAA | 1 cut(s) | 120 |