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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 196 |
| Acc16I | TGCGCA | 1 cut(s) | 906 |
| Acc36I | ACCTGC | 1 cut(s) | 196 |
| Acc65I | GGTACC | 1 cut(s) | 58 |
| AccB1I | GGYRCC | 2 cut(s) | 58, 1590 |
| AccBSI | CCGCTC | 1 cut(s) | 262 |
| AciI | CCGC | 7 cut(s) | 62, 262, 459, 662, 1130, 1452, 1499 |
| AclWI | GGATC | 6 cut(s) | 176, 189, 405, 494, 1277, 1290 |
| AcoI | YGGCCR | 1 cut(s) | 1561 |
| AcsI | RAATTY | 6 cut(s) | 47, 190, 325, 667, 736, 1039 |
| AfaI | GTAC | 4 cut(s) | 60, 243, 406, 463 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 151, 287, 1139 |
| AjnI | CCWGG | 3 cut(s) | 30, 144, 951 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 497, 529, 721, 753, 1311, 1343 |
| AleI | CACNNNNGTG | 1 cut(s) | 1482 |
| AluBI | AGCT | 5 cut(s) | 520, 970, 992, 1099, 1272 |
| AluI | AGCT | 5 cut(s) | 520, 970, 992, 1099, 1272 |
| Alw26I | GTCTC | 4 cut(s) | 364, 443, 644, 1608 |
| AlwI | GGATC | 6 cut(s) | 176, 189, 405, 494, 1277, 1290 |
| AoxI | GGCC | 3 cut(s) | 1056, 1561, 1616 |
| ApeKI | GCWGC | 1 cut(s) | 1381 |
| ApoI | RAATTY | 6 cut(s) | 47, 190, 325, 667, 736, 1039 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1202, 1234 |
| Asp718I | GGTACC | 1 cut(s) | 58 |
| AspLEI | GCGC | 1 cut(s) | 907 |
| AspS9I | GGNCC | 1 cut(s) | 560 |
| AsuC2I | CCSGG | 1 cut(s) | 1435 |
| AsuHPI | GGTGA | 3 cut(s) | 437, 863, 1370 |
| AsuNHI | GCTAGC | 1 cut(s) | 992 |
| AvaII | GGWCC | 1 cut(s) | 560 |
| BaeGI | GKGCMC | 1 cut(s) | 1595 |
| BalI | TGGCCA | 1 cut(s) | 1563 |
| BamHI | GGATCC | 2 cut(s) | 181, 1282 |
| BanI | GGYRCC | 2 cut(s) | 58, 1590 |
| BbvI | GCAGC | 1 cut(s) | 1368 |
| BccI | CCATC | 2 cut(s) | 784, 1048 |
| BceAI | ACGGC | 1 cut(s) | 1026 |
| BciT130I | CCWGG | 3 cut(s) | 32, 146, 953 |
| BciVI | GTATCC | 1 cut(s) | 238 |
| BcnI | CCSGG | 1 cut(s) | 1435 |
| BcoDI | GTCTC | 4 cut(s) | 364, 443, 644, 1608 |
| BfaI | CTAG | 7 cut(s) | 237, 345, 420, 993, 1250, 1301, 1416 |
| BfuAI | ACCTGC | 1 cut(s) | 196 |
| BfuI | GTATCC | 1 cut(s) | 238 |
| BglII | AGATCT | 1 cut(s) | 1426 |
| BisI | GCNGC | 3 cut(s) | 1130, 1382, 1453 |
| BlsI | GCNGC | 3 cut(s) | 1131, 1383, 1454 |
| BmcAI | AGTACT | 1 cut(s) | 406 |
| Bme1390I | CCNGG | 4 cut(s) | 32, 146, 953, 1435 |
| Bme18I | GGWCC | 1 cut(s) | 560 |
| BmgT120I | GGNCC | 1 cut(s) | 560 |
| BmiI | GGNNCC | 6 cut(s) | 60, 183, 306, 561, 1284, 1592 |
| BmrFI | CCNGG | 4 cut(s) | 32, 146, 953, 1435 |
| BmrI | ACTGGG | 1 cut(s) | 1196 |
| BmsI | GCATC | 2 cut(s) | 844, 1473 |
| BmtI | GCTAGC | 1 cut(s) | 996 |
| BmuI | ACTGGG | 1 cut(s) | 1196 |
| BpmI | CTGGAG | 1 cut(s) | 647 |
| Bpu10I | CCTNAGC | 1 cut(s) | 698 |
| BpuEI | CTTGAG | 2 cut(s) | 389, 956 |
| BpuMI | CCSGG | 1 cut(s) | 1435 |
| BsaBI | GATNNNNATC | 2 cut(s) | 396, 498 |
| BsaI | GGTCTC | 1 cut(s) | 364 |
| BsaJI | CCNNGG | 4 cut(s) | 30, 951, 1133, 1260 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 151, 287, 1139 |
| Bse1I | ACTGG | 1 cut(s) | 1191 |
| Bse3DI | GCAATG | 1 cut(s) | 577 |
| Bse8I | GATNNNNATC | 2 cut(s) | 396, 498 |
| BseBI | CCWGG | 3 cut(s) | 32, 146, 953 |
| BseDI | CCNNGG | 4 cut(s) | 30, 951, 1133, 1260 |
| BseGI | GGATG | 2 cut(s) | 118, 354 |
| BseJI | GATNNNNATC | 2 cut(s) | 396, 498 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 151, 287, 1139 |
| BseMI | GCAATG | 1 cut(s) | 577 |
| BseMII | CTCAG | 1 cut(s) | 712 |
| BseNI | ACTGG | 1 cut(s) | 1191 |
| BseSI | GKGCMC | 1 cut(s) | 1595 |
| BseXI | GCAGC | 1 cut(s) | 1368 |
| BseYI | CCCAGC | 2 cut(s) | 1455, 1585 |
| BsgI | GTGCAG | 2 cut(s) | 170, 1562 |
| BshFI | GGCC | 3 cut(s) | 1058, 1563, 1618 |
| BshNI | GGYRCC | 2 cut(s) | 58, 1590 |
| BsiSI | CCGG | 2 cut(s) | 1175, 1435 |
| BslFI | GGGAC | 5 cut(s) | 573, 934, 1278, 1472, 1488 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 151, 287, 1139 |
| BsmAI | GTCTC | 4 cut(s) | 364, 443, 644, 1608 |
| BsmFI | GGGAC | 5 cut(s) | 573, 934, 1278, 1472, 1488 |
| BsnI | GGCC | 3 cut(s) | 1058, 1563, 1618 |
| Bso31I | GGTCTC | 1 cut(s) | 364 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1595 |
| Bsp143I | GATC | 7 cut(s) | 181, 397, 499, 834, 1282, 1347, 1426 |
| BspACI | CCGC | 7 cut(s) | 62, 262, 459, 662, 1130, 1452, 1499 |
| BspANI | GGCC | 3 cut(s) | 1058, 1563, 1618 |
| BspCNI | CTCAG | 1 cut(s) | 711 |
| BspLI | GGNNCC | 6 cut(s) | 60, 183, 306, 561, 1284, 1592 |
| BspMI | ACCTGC | 1 cut(s) | 196 |
| BspOI | GCTAGC | 1 cut(s) | 996 |
| BspPI | GGATC | 6 cut(s) | 176, 189, 405, 494, 1277, 1290 |
| BspQI | GCTCTTC | 1 cut(s) | 1227 |
| BspT107I | GGYRCC | 2 cut(s) | 58, 1590 |
| BspTNI | GGTCTC | 1 cut(s) | 364 |
| BsrBI | CCGCTC | 1 cut(s) | 262 |
| BsrDI | GCAATG | 1 cut(s) | 577 |
| BsrI | ACTGG | 1 cut(s) | 1191 |
| BssECI | CCNNGG | 4 cut(s) | 30, 951, 1133, 1260 |
| BssMI | GATC | 7 cut(s) | 181, 397, 499, 834, 1282, 1347, 1426 |
| BssT1I | CCWWGG | 2 cut(s) | 1133, 1260 |
| Bst2UI | CCWGG | 3 cut(s) | 32, 146, 953 |
| Bst4CI | ACNGT | 6 cut(s) | 246, 877, 1358, 1481, 1540, 1602 |
| Bst6I | CTCTTC | 3 cut(s) | 275, 1227, 1314 |
| BstC8I | GCNNGC | 5 cut(s) | 639, 994, 1101, 1509, 1545 |
| BstDEI | CTNAG | 2 cut(s) | 656, 698 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 149 |
| BstF5I | GGATG | 2 cut(s) | 118, 354 |
| BstHHI | GCGC | 1 cut(s) | 907 |
| BstKTI | GATC | 7 cut(s) | 184, 400, 502, 837, 1285, 1350, 1429 |
| BstMAI | GTCTC | 4 cut(s) | 364, 443, 644, 1608 |
| BstMBI | GATC | 7 cut(s) | 181, 397, 499, 834, 1282, 1347, 1426 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1461 |
| BstNI | CCWGG | 3 cut(s) | 32, 146, 953 |
| BstSCI | CCNGG | 4 cut(s) | 30, 144, 951, 1433 |
| BstSLI | GKGCMC | 1 cut(s) | 1595 |
| BstV1I | GCAGC | 1 cut(s) | 1368 |
| BstX2I | RGATCY | 3 cut(s) | 181, 1282, 1426 |
| BstYI | RGATCY | 3 cut(s) | 181, 1282, 1426 |
| BsuI | GTATCC | 1 cut(s) | 238 |
| BsuRI | GGCC | 3 cut(s) | 1058, 1563, 1618 |
| BtsCI | GGATG | 2 cut(s) | 118, 354 |
| BtsIMutI | CAGTG | 1 cut(s) | 1486 |
| BveI | ACCTGC | 1 cut(s) | 196 |
| Cac8I | GCNNGC | 5 cut(s) | 639, 994, 1101, 1509, 1545 |
| CfoI | GCGC | 1 cut(s) | 907 |
| Cfr13I | GGNCC | 1 cut(s) | 560 |
| CseI | GACGC | 1 cut(s) | 1226 |
| Csp6I | GTAC | 4 cut(s) | 59, 242, 405, 462 |
| CviAII | CATG | 2 cut(s) | 682, 1608 |
| CviQI | GTAC | 4 cut(s) | 59, 242, 405, 462 |
| DdeI | CTNAG | 2 cut(s) | 656, 698 |
| DpnI | GATC | 7 cut(s) | 183, 399, 501, 836, 1284, 1349, 1428 |
| DpnII | GATC | 7 cut(s) | 181, 397, 499, 834, 1282, 1347, 1426 |
| EaeI | YGGCCR | 1 cut(s) | 1561 |
| Eam1104I | CTCTTC | 3 cut(s) | 275, 1227, 1314 |
| EarI | CTCTTC | 3 cut(s) | 275, 1227, 1314 |
| Eco130I | CCWWGG | 2 cut(s) | 1133, 1260 |
| Eco147I | AGGCCT | 1 cut(s) | 1618 |
| Eco31I | GGTCTC | 1 cut(s) | 364 |
| Eco47I | GGWCC | 1 cut(s) | 560 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 149 |
| EcoO109I | RGGNCCY | 1 cut(s) | 560 |
| EcoRI | GAATTC | 2 cut(s) | 47, 736 |
| EcoRII | CCWGG | 3 cut(s) | 30, 144, 951 |
| EcoT14I | CCWWGG | 2 cut(s) | 1133, 1260 |
| ErhI | CCWWGG | 2 cut(s) | 1133, 1260 |
| FaeI | CATG | 2 cut(s) | 685, 1611 |
| FaqI | GGGAC | 5 cut(s) | 573, 934, 1278, 1472, 1488 |
| FatI | CATG | 2 cut(s) | 681, 1607 |
| Fnu4HI | GCNGC | 3 cut(s) | 1130, 1382, 1453 |
| FokI | GGATG | 2 cut(s) | 125, 361 |
| Fsp4HI | GCNGC | 3 cut(s) | 1130, 1382, 1453 |
| FspBI | CTAG | 7 cut(s) | 237, 345, 420, 993, 1250, 1301, 1416 |
| FspI | TGCGCA | 1 cut(s) | 906 |
| GlaI | GCGC | 1 cut(s) | 906 |
| GluI | GCNGC | 3 cut(s) | 1130, 1382, 1453 |
| GsaI | CCCAGC | 2 cut(s) | 1459, 1589 |
| GsuI | CTGGAG | 1 cut(s) | 647 |
| HaeIII | GGCC | 3 cut(s) | 1058, 1563, 1618 |
| HapII | CCGG | 2 cut(s) | 1175, 1435 |
| HgaI | GACGC | 1 cut(s) | 1226 |
| HhaI | GCGC | 1 cut(s) | 907 |
| Hin1II | CATG | 2 cut(s) | 685, 1611 |
| Hin6I | GCGC | 1 cut(s) | 905 |
| HinP1I | GCGC | 1 cut(s) | 905 |
| HincII | GTYRAC | 1 cut(s) | 1374 |
| HindII | GTYRAC | 1 cut(s) | 1374 |
| HinfI | GANTC | 5 cut(s) | 265, 652, 724, 1224, 1253 |
| HpaI | GTTAAC | 1 cut(s) | 1374 |
| HpaII | CCGG | 2 cut(s) | 1175, 1435 |
| HphI | GGTGA | 3 cut(s) | 437, 863, 1370 |
| Hpy166II | GTNNAC | 3 cut(s) | 1003, 1207, 1374 |
| Hpy188I | TCNGA | 7 cut(s) | 20, 70, 174, 402, 1223, 1231, 1431 |
| Hpy188III | TCNNGA | 7 cut(s) | 44, 237, 368, 471, 608, 700, 1291 |
| Hpy8I | GTNNAC | 3 cut(s) | 1003, 1207, 1374 |
| Hpy99I | CGWCG | 1 cut(s) | 1540 |
| HpyAV | CCTTC | 8 cut(s) | 285, 573, 782, 877, 918, 1031, 1069, 1355 |
| HpyCH4III | ACNGT | 6 cut(s) | 246, 877, 1358, 1481, 1540, 1602 |
| HpyCH4IV | ACGT | 1 cut(s) | 1267 |
| HpyCH4V | TGCA | 8 cut(s) | 8, 105, 151, 205, 681, 962, 1384, 1543 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1461 |
| HpyF3I | CTNAG | 2 cut(s) | 656, 698 |
| HpySE526I | ACGT | 1 cut(s) | 1267 |
| Hsp92II | CATG | 2 cut(s) | 685, 1611 |
| HspAI | GCGC | 1 cut(s) | 905 |
| KpnI | GGTACC | 1 cut(s) | 62 |
| KspAI | GTTAAC | 1 cut(s) | 1374 |
| Kzo9I | GATC | 7 cut(s) | 181, 397, 499, 834, 1282, 1347, 1426 |
| LguI | GCTCTTC | 1 cut(s) | 1227 |
| Lsp1109I | GCAGC | 1 cut(s) | 1368 |
| LweI | GCATC | 2 cut(s) | 844, 1473 |
| MaeI | CTAG | 7 cut(s) | 237, 345, 420, 993, 1250, 1301, 1416 |
| MaeII | ACGT | 1 cut(s) | 1267 |
| MaeIII | GTNAC | 2 cut(s) | 522, 1523 |
| MalI | GATC | 7 cut(s) | 183, 399, 501, 836, 1284, 1349, 1428 |
| MbiI | CCGCTC | 1 cut(s) | 262 |
| MboI | GATC | 7 cut(s) | 181, 397, 499, 834, 1282, 1347, 1426 |
| MboII | GAAGA | 7 cut(s) | 262, 557, 878, 892, 901, 1244, 1331 |
| MflI | RGATCY | 3 cut(s) | 181, 1282, 1426 |
| MhlI | GDGCHC | 1 cut(s) | 1595 |
| MlsI | TGGCCA | 1 cut(s) | 1563 |
| MluNI | TGGCCA | 1 cut(s) | 1563 |
| MlyI | GAGTC | 3 cut(s) | 259, 646, 1218 |
| MmeI | TCCRAC | 2 cut(s) | 916, 1041 |
| Mox20I | TGGCCA | 1 cut(s) | 1563 |
| MscI | TGGCCA | 1 cut(s) | 1563 |
| MseI | TTAA | 3 cut(s) | 1373, 1530, 1621 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1482 |
| Msp20I | TGGCCA | 1 cut(s) | 1563 |
| MspA1I | CMGCKG | 1 cut(s) | 520 |
| MspI | CCGG | 2 cut(s) | 1175, 1435 |
| MspR9I | CCNGG | 4 cut(s) | 32, 146, 953, 1435 |
| MvaI | CCWGG | 3 cut(s) | 32, 146, 953 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1461 |
| NciI | CCSGG | 1 cut(s) | 1435 |
| NdeII | GATC | 7 cut(s) | 181, 397, 499, 834, 1282, 1347, 1426 |
| NheI | GCTAGC | 1 cut(s) | 992 |
| NlaIII | CATG | 2 cut(s) | 685, 1611 |
| NlaIV | GGNNCC | 6 cut(s) | 60, 183, 306, 561, 1284, 1592 |
| NmeAIII | GCCGAG | 1 cut(s) | 1475 |
| NmuCI | GTSAC | 1 cut(s) | 1523 |
| NsbI | TGCGCA | 1 cut(s) | 906 |
| OliI | CACNNNNGTG | 1 cut(s) | 1482 |
| PaqCI | CACCTGC | 1 cut(s) | 196 |
| PceI | AGGCCT | 1 cut(s) | 1618 |
| PciSI | GCTCTTC | 1 cut(s) | 1227 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1227 |
| PfeI | GAWTC | 2 cut(s) | 724, 1253 |
| PkrI | GCNGC | 3 cut(s) | 1131, 1383, 1454 |
| PleI | GAGTC | 3 cut(s) | 259, 646, 1218 |
| PpsI | GAGTC | 3 cut(s) | 259, 646, 1218 |
| PpuMI | RGGWCCY | 1 cut(s) | 560 |
| Psp5II | RGGWCCY | 1 cut(s) | 560 |
| Psp6I | CCWGG | 3 cut(s) | 30, 144, 951 |
| PspFI | CCCAGC | 2 cut(s) | 1455, 1585 |
| PspGI | CCWGG | 3 cut(s) | 30, 144, 951 |
| PspN4I | GGNNCC | 6 cut(s) | 60, 183, 306, 561, 1284, 1592 |
| PspPI | GGNCC | 1 cut(s) | 560 |
| PspPPI | RGGWCCY | 1 cut(s) | 560 |
| PsuI | RGATCY | 3 cut(s) | 181, 1282, 1426 |
| PvuII | CAGCTG | 1 cut(s) | 520 |
| RsaI | GTAC | 4 cut(s) | 60, 243, 406, 463 |
| RsaNI | GTAC | 4 cut(s) | 59, 242, 405, 462 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1482 |
| SapI | GCTCTTC | 1 cut(s) | 1227 |
| SaqAI | TTAA | 3 cut(s) | 1373, 1530, 1621 |
| SatI | GCNGC | 3 cut(s) | 1130, 1382, 1453 |
| Sau3AI | GATC | 7 cut(s) | 181, 397, 499, 834, 1282, 1347, 1426 |
| Sau96I | GGNCC | 1 cut(s) | 560 |
| ScaI | AGTACT | 1 cut(s) | 406 |
| SchI | GAGTC | 3 cut(s) | 259, 646, 1218 |
| ScrFI | CCNGG | 4 cut(s) | 32, 146, 953, 1435 |
| SduI | GDGCHC | 1 cut(s) | 1595 |
| SfaNI | GCATC | 2 cut(s) | 844, 1473 |
| SinI | GGWCC | 1 cut(s) | 560 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1482 |
| SmlI | CTYRAG | 2 cut(s) | 368, 971 |
| SmoI | CTYRAG | 2 cut(s) | 368, 971 |
| SseBI | AGGCCT | 1 cut(s) | 1618 |
| SsiI | CCGC | 7 cut(s) | 62, 262, 459, 662, 1130, 1452, 1499 |
| SspI | AATATT | 1 cut(s) | 980 |
| SspMI | CTAG | 7 cut(s) | 237, 345, 420, 993, 1250, 1301, 1416 |
| StuI | AGGCCT | 1 cut(s) | 1618 |
| StyD4I | CCNGG | 4 cut(s) | 30, 144, 951, 1433 |
| StyI | CCWWGG | 2 cut(s) | 1133, 1260 |
| TaaI | ACNGT | 6 cut(s) | 246, 877, 1358, 1481, 1540, 1602 |
| TaiI | ACGT | 1 cut(s) | 1270 |
| TaqI | TCGA | 4 cut(s) | 1183, 1256, 1346, 1398 |
| TatI | WGTACW | 2 cut(s) | 241, 404 |
| TauI | GCSGC | 2 cut(s) | 1132, 1455 |
| TfiI | GAWTC | 2 cut(s) | 724, 1253 |
| Tru1I | TTAA | 3 cut(s) | 1373, 1530, 1621 |
| Tru9I | TTAA | 3 cut(s) | 1373, 1530, 1621 |
| TscAI | CASTG | 1 cut(s) | 1486 |
| TseFI | GTSAC | 1 cut(s) | 1523 |
| TseI | GCWGC | 1 cut(s) | 1381 |
| Tsp45I | GTSAC | 1 cut(s) | 1523 |
| TspDTI | ATGAA | 4 cut(s) | 40, 262, 365, 858 |
| TspRI | CASTG | 1 cut(s) | 1486 |
| VpaK11BI | GGWCC | 1 cut(s) | 560 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 149 |
| XapI | RAATTY | 6 cut(s) | 47, 190, 325, 667, 736, 1039 |
| XbaI | TCTAGA | 1 cut(s) | 236 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1293 |
| XspI | CTAG | 7 cut(s) | 237, 345, 420, 993, 1250, 1301, 1416 |
| ZrmI | AGTACT | 1 cut(s) | 406 |