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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 91 |
| AciI | CCGC | 3 cut(s) | 91, 288, 502 |
| AclWI | GGATC | 2 cut(s) | 234, 323 |
| AcsI | RAATTY | 2 cut(s) | 154, 507 |
| AfaI | GTAC | 3 cut(s) | 32, 235, 292 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 116 |
| AgsI | TTSAA | 5 cut(s) | 49, 107, 272, 396, 439 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 326, 358 |
| AluBI | AGCT | 1 cut(s) | 349 |
| AluI | AGCT | 1 cut(s) | 349 |
| Alw26I | GTCTC | 2 cut(s) | 193, 475 |
| AlwI | GGATC | 2 cut(s) | 234, 323 |
| ApoI | RAATTY | 2 cut(s) | 154, 507 |
| AspS9I | GGNCC | 1 cut(s) | 389 |
| AsuHPI | GGTGA | 1 cut(s) | 266 |
| AvaII | GGWCC | 1 cut(s) | 389 |
| BceAI | ACGGC | 1 cut(s) | 58 |
| BciVI | GTATCC | 1 cut(s) | 27 |
| BcoDI | GTCTC | 2 cut(s) | 193, 475 |
| BfaI | CTAG | 3 cut(s) | 26, 174, 249 |
| BfuI | GTATCC | 1 cut(s) | 27 |
| BmcAI | AGTACT | 1 cut(s) | 235 |
| Bme18I | GGWCC | 1 cut(s) | 389 |
| BmgT120I | GGNCC | 1 cut(s) | 389 |
| BmiI | GGNNCC | 2 cut(s) | 135, 390 |
| BpmI | CTGGAG | 1 cut(s) | 478 |
| BpuEI | CTTGAG | 1 cut(s) | 218 |
| BsaBI | GATNNNNATC | 2 cut(s) | 225, 327 |
| BsaI | GGTCTC | 1 cut(s) | 193 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 116 |
| Bse3DI | GCAATG | 1 cut(s) | 406 |
| Bse8I | GATNNNNATC | 2 cut(s) | 225, 327 |
| BseGI | GGATG | 1 cut(s) | 183 |
| BseJI | GATNNNNATC | 2 cut(s) | 225, 327 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 116 |
| BseMI | GCAATG | 1 cut(s) | 406 |
| BslFI | GGGAC | 2 cut(s) | 292, 402 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 116 |
| BsmAI | GTCTC | 2 cut(s) | 193, 475 |
| BsmFI | GGGAC | 2 cut(s) | 292, 402 |
| Bso31I | GGTCTC | 1 cut(s) | 193 |
| Bsp143I | GATC | 2 cut(s) | 226, 328 |
| BspACI | CCGC | 3 cut(s) | 91, 288, 502 |
| BspLI | GGNNCC | 2 cut(s) | 135, 390 |
| BspPI | GGATC | 2 cut(s) | 234, 323 |
| BspTNI | GGTCTC | 1 cut(s) | 193 |
| BsrBI | CCGCTC | 1 cut(s) | 91 |
| BsrDI | GCAATG | 1 cut(s) | 406 |
| BssMI | GATC | 2 cut(s) | 226, 328 |
| Bst6I | CTCTTC | 1 cut(s) | 104 |
| BstC8I | GCNNGC | 1 cut(s) | 470 |
| BstF5I | GGATG | 1 cut(s) | 183 |
| BstKTI | GATC | 2 cut(s) | 229, 331 |
| BstMAI | GTCTC | 2 cut(s) | 193, 475 |
| BstMBI | GATC | 2 cut(s) | 226, 328 |
| BsuI | GTATCC | 1 cut(s) | 27 |
| BtsCI | GGATG | 1 cut(s) | 183 |
| Cac8I | GCNNGC | 1 cut(s) | 470 |
| Cfr13I | GGNCC | 1 cut(s) | 389 |
| Csp6I | GTAC | 3 cut(s) | 31, 234, 291 |
| CviJI | RGCY | 3 cut(s) | 349, 381, 472 |
| CviKI_1 | RGCY | 3 cut(s) | 349, 381, 472 |
| CviQI | GTAC | 3 cut(s) | 31, 234, 291 |
| DpnI | GATC | 2 cut(s) | 228, 330 |
| DpnII | GATC | 2 cut(s) | 226, 328 |
| Eam1104I | CTCTTC | 1 cut(s) | 104 |
| EarI | CTCTTC | 1 cut(s) | 104 |
| Eco31I | GGTCTC | 1 cut(s) | 193 |
| Eco47I | GGWCC | 1 cut(s) | 389 |
| EcoO109I | RGGNCCY | 1 cut(s) | 389 |
| FaiI | YATR | 2 cut(s) | 171, 413 |
| FaqI | GGGAC | 2 cut(s) | 292, 402 |
| FokI | GGATG | 1 cut(s) | 190 |
| FspBI | CTAG | 3 cut(s) | 26, 174, 249 |
| GsuI | CTGGAG | 1 cut(s) | 478 |
| HinfI | GANTC | 2 cut(s) | 94, 483 |
| HphI | GGTGA | 1 cut(s) | 266 |
| Hpy188I | TCNGA | 1 cut(s) | 231 |
| Hpy188III | TCNNGA | 4 cut(s) | 26, 197, 300, 439 |
| HpyAV | CCTTC | 3 cut(s) | 4, 114, 402 |
| Kzo9I | GATC | 2 cut(s) | 226, 328 |
| LpnPI | CCDG | 5 cut(s) | 19, 146, 359, 429, 442 |
| MaeI | CTAG | 3 cut(s) | 26, 174, 249 |
| MaeIII | GTNAC | 1 cut(s) | 351 |
| MalI | GATC | 2 cut(s) | 228, 330 |
| MbiI | CCGCTC | 1 cut(s) | 91 |
| MboI | GATC | 2 cut(s) | 226, 328 |
| MboII | GAAGA | 2 cut(s) | 91, 386 |
| MluCI | AATT | 7 cut(s) | 63, 141, 154, 273, 362, 419, 507 |
| MlyI | GAGTC | 2 cut(s) | 88, 477 |
| MnlI | CCTC | 5 cut(s) | 110, 199, 217, 379, 453 |
| MspA1I | CMGCKG | 1 cut(s) | 349 |
| NdeII | GATC | 2 cut(s) | 226, 328 |
| NlaIV | GGNNCC | 2 cut(s) | 135, 390 |
| PleI | GAGTC | 2 cut(s) | 88, 477 |
| PpsI | GAGTC | 2 cut(s) | 88, 477 |
| PpuMI | RGGWCCY | 1 cut(s) | 389 |
| Psp5II | RGGWCCY | 1 cut(s) | 389 |
| PspN4I | GGNNCC | 2 cut(s) | 135, 390 |
| PspPI | GGNCC | 1 cut(s) | 389 |
| PspPPI | RGGWCCY | 1 cut(s) | 389 |
| PvuII | CAGCTG | 1 cut(s) | 349 |
| RsaI | GTAC | 3 cut(s) | 32, 235, 292 |
| RsaNI | GTAC | 3 cut(s) | 31, 234, 291 |
| Sau3AI | GATC | 2 cut(s) | 226, 328 |
| Sau96I | GGNCC | 1 cut(s) | 389 |
| ScaI | AGTACT | 1 cut(s) | 235 |
| SchI | GAGTC | 2 cut(s) | 88, 477 |
| SetI | ASST | 6 cut(s) | 38, 210, 351, 394, 418, 464 |
| SinI | GGWCC | 1 cut(s) | 389 |
| SmlI | CTYRAG | 1 cut(s) | 197 |
| SmoI | CTYRAG | 1 cut(s) | 197 |
| Sse9I | AATT | 7 cut(s) | 63, 141, 154, 273, 362, 419, 507 |
| SsiI | CCGC | 3 cut(s) | 91, 288, 502 |
| SspMI | CTAG | 3 cut(s) | 26, 174, 249 |
| TasI | AATT | 7 cut(s) | 63, 141, 154, 273, 362, 419, 507 |
| TatI | WGTACW | 2 cut(s) | 30, 233 |
| TspDTI | ATGAA | 2 cut(s) | 91, 194 |
| VpaK11BI | GGWCC | 1 cut(s) | 389 |
| XapI | RAATTY | 2 cut(s) | 154, 507 |
| XbaI | TCTAGA | 1 cut(s) | 25 |
| XspI | CTAG | 3 cut(s) | 26, 174, 249 |
| ZrmI | AGTACT | 1 cut(s) | 235 |