TdcA1-ORF2 protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 648 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 435 |
| AccB1I | GGYRCC | 1 cut(s) | 32 |
| AclI | AACGTT | 1 cut(s) | 24 |
| AclWI | GGATC | 2 cut(s) | 319, 882 |
| AcsI | RAATTY | 1 cut(s) | 106 |
| AcuI | CTGAAG | 4 cut(s) | 291, 674, 744, 976 |
| AfaI | GTAC | 4 cut(s) | 81, 181, 391, 827 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 203 |
| AflIII | ACRYGT | 3 cut(s) | 76, 223, 579 |
| AgsI | TTSAA | 5 cut(s) | 280, 412, 809, 818, 1030 |
| AjiI | CACGTC | 1 cut(s) | 77 |
| AluBI | AGCT | 2 cut(s) | 385, 652 |
| AluI | AGCT | 2 cut(s) | 385, 652 |
| Alw21I | GWGCWC | 1 cut(s) | 749 |
| Alw26I | GTCTC | 2 cut(s) | 588, 893 |
| Alw44I | GTGCAC | 1 cut(s) | 745 |
| AlwI | GGATC | 2 cut(s) | 319, 882 |
| AoxI | GGCC | 1 cut(s) | 194 |
| ApaLI | GTGCAC | 1 cut(s) | 745 |
| ApeKI | GCWGC | 3 cut(s) | 382, 776, 965 |
| ApoI | RAATTY | 1 cut(s) | 106 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 768 |
| AspS9I | GGNCC | 2 cut(s) | 323, 437 |
| AsuHPI | GGTGA | 2 cut(s) | 218, 466 |
| AsuII | TTCGAA | 1 cut(s) | 20 |
| AvaII | GGWCC | 2 cut(s) | 323, 437 |
| BaeGI | GKGCMC | 2 cut(s) | 37, 749 |
| BanI | GGYRCC | 1 cut(s) | 32 |
| BbsI | GAAGAC | 1 cut(s) | 5 |
| Bbv12I | GWGCWC | 1 cut(s) | 749 |
| BbvI | GCAGC | 3 cut(s) | 394, 763, 977 |
| BccI | CCATC | 2 cut(s) | 259, 908 |
| BceAI | ACGGC | 1 cut(s) | 86 |
| BciVI | GTATCC | 1 cut(s) | 950 |
| BclI | TGATCA | 1 cut(s) | 546 |
| BcoDI | GTCTC | 2 cut(s) | 588, 893 |
| BfaI | CTAG | 4 cut(s) | 177, 288, 759, 797 |
| BfmI | CTRYAG | 2 cut(s) | 318, 1018 |
| BfuI | GTATCC | 1 cut(s) | 950 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 41 |
| BisI | GCNGC | 3 cut(s) | 383, 777, 966 |
| BlsI | GCNGC | 3 cut(s) | 384, 778, 967 |
| Bme18I | GGWCC | 2 cut(s) | 323, 437 |
| BmgBI | CACGTC | 1 cut(s) | 77 |
| BmgT120I | GGNCC | 2 cut(s) | 323, 437 |
| BmiI | GGNNCC | 3 cut(s) | 34, 485, 1011 |
| BmsI | GCATC | 3 cut(s) | 274, 783, 879 |
| BoxI | GACNNNNGTC | 1 cut(s) | 296 |
| BpiI | GAAGAC | 1 cut(s) | 5 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 659, 691 |
| Bpu14I | TTCGAA | 1 cut(s) | 20 |
| BsaJI | CCNNGG | 2 cut(s) | 307, 590 |
| BsaWI | WCCGGW | 1 cut(s) | 29 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 203 |
| BseDI | CCNNGG | 2 cut(s) | 307, 590 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 203 |
| BseMII | CTCAG | 2 cut(s) | 888, 1013 |
| BseSI | GKGCMC | 2 cut(s) | 37, 749 |
| BseXI | GCAGC | 3 cut(s) | 394, 763, 977 |
| BsgI | GTGCAG | 1 cut(s) | 762 |
| BshFI | GGCC | 1 cut(s) | 196 |
| BshNI | GGYRCC | 1 cut(s) | 32 |
| BsiHKAI | GWGCWC | 1 cut(s) | 749 |
| BsiSI | CCGG | 1 cut(s) | 30 |
| BslFI | GGGAC | 2 cut(s) | 863, 931 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 203 |
| BsmAI | GTCTC | 2 cut(s) | 588, 893 |
| BsmFI | GGGAC | 2 cut(s) | 863, 931 |
| BsmI | GAATGC | 1 cut(s) | 223 |
| BsnI | GGCC | 1 cut(s) | 196 |
| Bsp119I | TTCGAA | 1 cut(s) | 20 |
| Bsp1286I | GDGCHC | 2 cut(s) | 37, 749 |
| Bsp1407I | TGTACA | 1 cut(s) | 79 |
| Bsp143I | GATC | 3 cut(s) | 311, 546, 874 |
| BspANI | GGCC | 1 cut(s) | 196 |
| BspCNI | CTCAG | 2 cut(s) | 889, 1012 |
| BspLI | GGNNCC | 3 cut(s) | 34, 485, 1011 |
| BspPI | GGATC | 2 cut(s) | 319, 882 |
| BspT104I | TTCGAA | 1 cut(s) | 20 |
| BspT107I | GGYRCC | 1 cut(s) | 32 |
| BsrGI | TGTACA | 1 cut(s) | 79 |
| BssECI | CCNNGG | 2 cut(s) | 307, 590 |
| BssMI | GATC | 3 cut(s) | 311, 546, 874 |
| BssT1I | CCWWGG | 2 cut(s) | 307, 590 |
| Bst4CI | ACNGT | 3 cut(s) | 202, 628, 984 |
| BstAUI | TGTACA | 1 cut(s) | 79 |
| BstBI | TTCGAA | 1 cut(s) | 20 |
| BstDEI | CTNAG | 3 cut(s) | 564, 897, 999 |
| BstKTI | GATC | 3 cut(s) | 314, 549, 877 |
| BstMAI | GTCTC | 2 cut(s) | 588, 893 |
| BstMBI | GATC | 3 cut(s) | 311, 546, 874 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 41, 382, 658, 667 |
| BstNSI | RCATGY | 1 cut(s) | 583 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 296 |
| BstSFI | CTRYAG | 2 cut(s) | 318, 1018 |
| BstSLI | GKGCMC | 2 cut(s) | 37, 749 |
| BstV1I | GCAGC | 3 cut(s) | 394, 763, 977 |
| BstV2I | GAAGAC | 1 cut(s) | 5 |
| BstX2I | RGATCY | 1 cut(s) | 311 |
| BstYI | RGATCY | 1 cut(s) | 311 |
| BsuI | GTATCC | 1 cut(s) | 950 |
| BsuRI | GGCC | 1 cut(s) | 196 |
| BtrI | CACGTC | 1 cut(s) | 77 |
| BtsIMutI | CAGTG | 2 cut(s) | 236, 633 |
| Cfr13I | GGNCC | 2 cut(s) | 323, 437 |
| Csp6I | GTAC | 4 cut(s) | 80, 180, 390, 826 |
| CviAII | CATG | 3 cut(s) | 377, 488, 580 |
| CviQI | GTAC | 4 cut(s) | 80, 180, 390, 826 |
| DdeI | CTNAG | 3 cut(s) | 564, 897, 999 |
| DpnI | GATC | 3 cut(s) | 313, 548, 876 |
| DpnII | GATC | 3 cut(s) | 311, 546, 874 |
| DraI | TTTAAA | 1 cut(s) | 360 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 435 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 435 |
| Eco130I | CCWWGG | 2 cut(s) | 307, 590 |
| Eco147I | AGGCCT | 1 cut(s) | 196 |
| Eco32I | GATATC | 1 cut(s) | 304 |
| Eco47I | GGWCC | 2 cut(s) | 323, 437 |
| Eco57I | CTGAAG | 4 cut(s) | 291, 674, 744, 976 |
| EcoRV | GATATC | 1 cut(s) | 304 |
| EcoT14I | CCWWGG | 2 cut(s) | 307, 590 |
| ErhI | CCWWGG | 2 cut(s) | 307, 590 |
| FaeI | CATG | 3 cut(s) | 380, 491, 583 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 108, 140 |
| FaqI | GGGAC | 2 cut(s) | 863, 931 |
| FatI | CATG | 3 cut(s) | 376, 487, 579 |
| FauNDI | CATATG | 1 cut(s) | 442 |
| FbaI | TGATCA | 1 cut(s) | 546 |
| Fnu4HI | GCNGC | 3 cut(s) | 383, 777, 966 |
| Fsp4HI | GCNGC | 3 cut(s) | 383, 777, 966 |
| FspBI | CTAG | 4 cut(s) | 177, 288, 759, 797 |
| GluI | GCNGC | 3 cut(s) | 383, 777, 966 |
| HaeIII | GGCC | 1 cut(s) | 196 |
| HapII | CCGG | 1 cut(s) | 30 |
| Hin1II | CATG | 3 cut(s) | 380, 491, 583 |
| HinfI | GANTC | 4 cut(s) | 209, 471, 919, 997 |
| HpaII | CCGG | 1 cut(s) | 30 |
| HphI | GGTGA | 2 cut(s) | 218, 466 |
| Hpy166II | GTNNAC | 2 cut(s) | 80, 747 |
| Hpy188I | TCNGA | 1 cut(s) | 483 |
| Hpy188III | TCNNGA | 5 cut(s) | 134, 326, 412, 911, 994 |
| Hpy8I | GTNNAC | 2 cut(s) | 80, 747 |
| Hpy99I | CGWCG | 1 cut(s) | 78 |
| HpyAV | CCTTC | 4 cut(s) | 105, 266, 508, 917 |
| HpyCH4III | ACNGT | 3 cut(s) | 202, 628, 984 |
| HpyCH4IV | ACGT | 4 cut(s) | 10, 24, 76, 225 |
| HpyCH4V | TGCA | 9 cut(s) | 115, 221, 376, 446, 491, 747, 779, 803, 950 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 41, 382, 658, 667 |
| HpyF3I | CTNAG | 3 cut(s) | 564, 897, 999 |
| HpySE526I | ACGT | 4 cut(s) | 10, 24, 76, 225 |
| Hsp92II | CATG | 3 cut(s) | 380, 491, 583 |
| Ksp22I | TGATCA | 1 cut(s) | 546 |
| Kzo9I | GATC | 3 cut(s) | 311, 546, 874 |
| LmnI | GCTCC | 1 cut(s) | 857 |
| Lsp1109I | GCAGC | 3 cut(s) | 394, 763, 977 |
| LweI | GCATC | 3 cut(s) | 274, 783, 879 |
| MaeI | CTAG | 4 cut(s) | 177, 288, 759, 797 |
| MaeII | ACGT | 4 cut(s) | 10, 24, 76, 225 |
| MaeIII | GTNAC | 3 cut(s) | 55, 206, 403 |
| MalI | GATC | 3 cut(s) | 313, 548, 876 |
| MboI | GATC | 3 cut(s) | 311, 546, 874 |
| MboII | GAAGA | 6 cut(s) | 5, 542, 667, 737, 740, 776 |
| MfeI | CAATTG | 1 cut(s) | 804 |
| MflI | RGATCY | 1 cut(s) | 311 |
| MhlI | GDGCHC | 2 cut(s) | 37, 749 |
| MluCI | AATT | 5 cut(s) | 106, 465, 671, 804, 866 |
| MlyI | GAGTC | 3 cut(s) | 203, 913, 991 |
| MmeI | TCCRAC | 1 cut(s) | 1037 |
| MnlI | CCTC | 5 cut(s) | 157, 347, 504, 679, 1016 |
| MroXI | GAANNNNTTC | 1 cut(s) | 768 |
| MseI | TTAA | 6 cut(s) | 359, 597, 717, 837, 885, 977 |
| MslI | CAYNNNNRTG | 2 cut(s) | 578, 711 |
| MspI | CCGG | 1 cut(s) | 30 |
| MunI | CAATTG | 1 cut(s) | 804 |
| Mva1269I | GAATGC | 1 cut(s) | 223 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 41, 382, 658, 667 |
| NdeI | CATATG | 1 cut(s) | 442 |
| NdeII | GATC | 3 cut(s) | 311, 546, 874 |
| NlaIII | CATG | 3 cut(s) | 380, 491, 583 |
| NlaIV | GGNNCC | 3 cut(s) | 34, 485, 1011 |
| NmuCI | GTSAC | 3 cut(s) | 55, 206, 403 |
| NspI | RCATGY | 1 cut(s) | 583 |
| NspV | TTCGAA | 1 cut(s) | 20 |
| PceI | AGGCCT | 1 cut(s) | 196 |
| PciI | ACATGT | 1 cut(s) | 579 |
| PctI | GAATGC | 1 cut(s) | 223 |
| PdmI | GAANNNNTTC | 1 cut(s) | 768 |
| PfeI | GAWTC | 1 cut(s) | 471 |
| PkrI | GCNGC | 3 cut(s) | 384, 778, 967 |
| PleI | GAGTC | 3 cut(s) | 203, 913, 991 |
| PpsI | GAGTC | 3 cut(s) | 203, 913, 991 |
| PscI | ACATGT | 1 cut(s) | 579 |
| PshAI | GACNNNNGTC | 1 cut(s) | 296 |
| PsiI | TTATAA | 1 cut(s) | 648 |
| Psp1406I | AACGTT | 1 cut(s) | 24 |
| PspN4I | GGNNCC | 3 cut(s) | 34, 485, 1011 |
| PspPI | GGNCC | 2 cut(s) | 323, 437 |
| PsuI | RGATCY | 1 cut(s) | 311 |
| RsaI | GTAC | 4 cut(s) | 81, 181, 391, 827 |
| RsaNI | GTAC | 4 cut(s) | 80, 180, 390, 826 |
| RseI | CAYNNNNRTG | 2 cut(s) | 578, 711 |
| SaqAI | TTAA | 6 cut(s) | 359, 597, 717, 837, 885, 977 |
| SatI | GCNGC | 3 cut(s) | 383, 777, 966 |
| Sau3AI | GATC | 3 cut(s) | 311, 546, 874 |
| Sau96I | GGNCC | 2 cut(s) | 323, 437 |
| SchI | GAGTC | 3 cut(s) | 203, 913, 991 |
| SduI | GDGCHC | 2 cut(s) | 37, 749 |
| SfaNI | GCATC | 3 cut(s) | 274, 783, 879 |
| SfcI | CTRYAG | 2 cut(s) | 318, 1018 |
| SfuI | TTCGAA | 1 cut(s) | 20 |
| SinI | GGWCC | 2 cut(s) | 323, 437 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 578, 711 |
| Sse9I | AATT | 5 cut(s) | 106, 465, 671, 804, 866 |
| SseBI | AGGCCT | 1 cut(s) | 196 |
| SspMI | CTAG | 4 cut(s) | 177, 288, 759, 797 |
| StuI | AGGCCT | 1 cut(s) | 196 |
| StyI | CCWWGG | 2 cut(s) | 307, 590 |
| TaaI | ACNGT | 3 cut(s) | 202, 628, 984 |
| TaiI | ACGT | 4 cut(s) | 13, 27, 79, 228 |
| TaqI | TCGA | 4 cut(s) | 5, 20, 300, 922 |
| TasI | AATT | 5 cut(s) | 106, 465, 671, 804, 866 |
| TatI | WGTACW | 2 cut(s) | 79, 825 |
| TfiI | GAWTC | 1 cut(s) | 471 |
| Tru1I | TTAA | 6 cut(s) | 359, 597, 717, 837, 885, 977 |
| Tru9I | TTAA | 6 cut(s) | 359, 597, 717, 837, 885, 977 |
| TscAI | CASTG | 2 cut(s) | 236, 633 |
| TseFI | GTSAC | 3 cut(s) | 55, 206, 403 |
| TseI | GCWGC | 3 cut(s) | 382, 776, 965 |
| Tsp45I | GTSAC | 3 cut(s) | 55, 206, 403 |
| TspDTI | ATGAA | 6 cut(s) | 105, 135, 230, 477, 944, 954 |
| TspGWI | ACGGA | 1 cut(s) | 632 |
| TspRI | CASTG | 2 cut(s) | 236, 633 |
| VneI | GTGCAC | 1 cut(s) | 745 |
| VpaK11BI | GGWCC | 2 cut(s) | 323, 437 |
| XapI | RAATTY | 1 cut(s) | 106 |
| XceI | RCATGY | 1 cut(s) | 583 |
| XmnI | GAANNNNTTC | 1 cut(s) | 768 |
| XspI | CTAG | 4 cut(s) | 177, 288, 759, 797 |