Rmu_sc0009185.1_g000010

Domain of unknown function (DUF4216)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009185.1
OG0000224
Physical Location & Seq
Forward (+)
41829 .. 47206
5378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009185.1_g000010.1.cds

Sequence Viewer

Length: 867 bp
atgctcctcagagcttactacgtagcggaagtcataacaatggcaaagccaacaaaatttgcttcctacccgttcaactaccaacaagctacctcaacttcaaccacgattatcctgacctgcaggattacccctacaccaatagaatggtgcaccgtgtttccacacaacaaaacccgaggatgccacagagacaaccagaaggagaagaagaaccagctgaccaagtttcacggccagagaccaccggagctacttgcactatatgaggacgaaccttttatacttgcaagtgaagcgcaacaagtgttttatctagatgattataagttgggccctgaatggaaagttgtccaaaaagtacatacgagacatgtgtgggatgtcccagaggtagatgatgaggatgaagaggtagatgaggaaaattttgatcaagtggctaataatgatgtatatcagcaagatgaattcaatgaaattcaatggtcggttcaagaagaaggtgccactgtgacaaaacggcgacatgtacaagcaaatgaaccttcacttccaaattctttgcaagatgaacttgatgttcctgatatgcctactgcaaatagaactacccaaaggactattggtagtaccattatccaaaggcctactgttggtccggaaaaaaaagtgcgtggaaagacaagaggtttaaatgctgataaggtgcatgagcagtttggtactaaaatacctgtgactttcaagaaagaaaatggcggacccacaggtccatatgcagaaatgtttgctaatgaaattggattcaccattcggaaccatgcacctctgaatgtaaaaaattggaaggaagtaaaaccataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transposase_21

Protein Analysis

288

Amino Acids

33.13

Weight (kDa)

5.69

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000224)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g08145 FvH4_4g05492 FvH4_4g15892
pyrus_communis pycom07g09690 pycom16g18380
rosa_chinensis RchiOBHm_Chr0c17g0499961 RchiOBHm_Chr1g0345631 RchiOBHm_Chr1g0345641 RchiOBHm_Chr1g0381191 RchiOBHm_Chr2g0102381 RchiOBHm_Chr2g0106131 RchiOBHm_Chr2g0148461 RchiOBHm_Chr2g0148471 RchiOBHm_Chr2g0157121 RchiOBHm_Chr3g0476041 RchiOBHm_Chr3g0476051 RchiOBHm_Chr3g0486961 RchiOBHm_Chr3g0487551 RchiOBHm_Chr4g0385011 RchiOBHm_Chr4g0410891 RchiOBHm_Chr4g0415291 RchiOBHm_Chr4g0427801 RchiOBHm_Chr4g0427811 RchiOBHm_Chr5g0001961 RchiOBHm_Chr5g0009841 RchiOBHm_Chr5g0025231 RchiOBHm_Chr6g0272461 RchiOBHm_Chr7g0199391 RchiOBHm_Chr7g0214031
rosa_laevigata RLG00000000706 RLG00000000749 RLG00000000750 RLG00000001166 RLG00000001748 RLG00000008952 RLG00000009088 RLG00000009090 RLG00000009094 RLG00000015391 RLG00000016519 RLG00000016520 RLG00000017174 RLG00000018887 RLG00000023058 RLG00000023059 RLG00000028282 RLG00000028531 RLG00000030444 RLG00000034592 RLG00000036555
rosa_multiflora Rmu_sc0000045.1_g000015 Rmu_sc0000157.1_g000011 Rmu_sc0000493.1_g000080 Rmu_sc0001307.1_g000027 Rmu_sc0001470.1_g000010 Rmu_sc0002277.1_g000022 Rmu_sc0002365.1_g000027 Rmu_sc0002693.1_g000008 Rmu_sc0002833.1_g000051 Rmu_sc0003275.1_g000066 Rmu_sc0003286.1_g000008 Rmu_sc0004234.1_g000035 Rmu_sc0004546.1_g000001 Rmu_sc0005058.1_g000004 Rmu_sc0007025.1_g000015 Rmu_sc0008144.1_g000006 Rmu_sc0008688.1_g000001 Rmu_sc0009185.1_g000010 Rmu_sc0020638.1_g000001
rosa_roxburghii Rroxscaffold_158G00443750 Rroxscaffold_1G00001770 Rroxscaffold_1G00002620 Rroxscaffold_1G00024490 Rroxscaffold_1G00035180 Rroxscaffold_1G00035190 Rroxscaffold_1G00061370 Rroxscaffold_1G00075590 Rroxscaffold_2G00110960 Rroxscaffold_2G00121340 Rroxscaffold_2G00128850 Rroxscaffold_2G00142580 Rroxscaffold_2G00142590 Rroxscaffold_3G00223630 Rroxscaffold_3G00263220 Rroxscaffold_4G00310820 Rroxscaffold_5G00336270 Rroxscaffold_5G00336280 Rroxscaffold_5G00336320 Rroxscaffold_5G00349820 Rroxscaffold_6G00395720 Rroxscaffold_6G00395910 Rroxscaffold_6G00415660 Rroxscaffold_7G00179770 Rroxscaffold_7G00184390 Rroxscaffold_7G00189270 Rroxscaffold_7G00197240 Rroxscaffold_8G00436850 Rroxscaffold_8G00436860 Rroxscaffold_8G00436880
rosa_rugosa Rorug01G0118400 Rorug02G0048900 Rorug02G0185600 Rorug02G0222600 Rorug03G0152800 Rorug04G0047600 Rorug04G0446400 Rorug05G0118900 Rorug05G0359700 Rorug05G0374800 Rorug05G0374900 Rorug05G0375000 Rorug05G0375100 Rorug05G0418900 Rorug05G0540000 Rorug05G0540000 Rorug05G0540100 Rorug06G0358700 Rorug06G0358700 Rorug06G0408700 Rorug06G0408700 Rorug06G0408800 Rorug06G0408900 Rorug07G0203000
rosa_samantha Rh1CG190700 Rh1CG196300 Rh2BG566600 Rh2CG188900 Rh3AG090900 Rh3AG278200 Rh3BG233900 Rh3CG094300 Rh4BG202100 Rh5BG543100 Rh5CG566000 Rh5DG397500 Rh7CG432800
rosa_wichuraiana Rw0G017850 Rw1G010340 Rw1G016290 Rw2G018520 Rw3G008970 Rw5G028090 Rw5G043930 Rw6G014660 Rw6G030340 Rw7G038240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
AasI GACNNNNNNGTC 1 cut(s) 771
Acc36I ACCTGC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 506
AccIII TCCGGA 1 cut(s) 661
AciI CCGC 2 cut(s) 26, 762
AcoI YGGCCR 1 cut(s) 235
AcsI RAATTY 5 cut(s) 56, 427, 470, 480, 559
AfaI GTAC 4 cut(s) 363, 534, 634, 727
AfiI CCNNNNNNNGG 1 cut(s) 656
AflIII ACRYGT 2 cut(s) 373, 529
AgsI TTSAA 6 cut(s) 76, 102, 475, 485, 497, 748
AluBI AGCT 4 cut(s) 14, 89, 220, 253
AluI AGCT 4 cut(s) 14, 89, 220, 253
Alw21I GWGCWC 1 cut(s) 155
Alw26I GTCTC 3 cut(s) 186, 235, 364
Alw44I GTGCAC 1 cut(s) 151
Ama87I CYCGRG 1 cut(s) 177
Aor13HI TCCGGA 1 cut(s) 661
AoxI GGCC 3 cut(s) 235, 334, 647
ApaI GGGCCC 1 cut(s) 338
ApaLI GTGCAC 1 cut(s) 151
ApoI RAATTY 5 cut(s) 56, 427, 470, 480, 559
AspLEI GCGC 1 cut(s) 301
AspS9I GGNCC 5 cut(s) 334, 335, 659, 764, 773
AsuHPI GGTGA 1 cut(s) 802
AvaI CYCGRG 1 cut(s) 177
AvaII GGWCC 3 cut(s) 659, 764, 773
BaeGI GKGCMC 2 cut(s) 155, 338
BanI GGYRCC 1 cut(s) 506
BanII GRGCYC 1 cut(s) 338
Bbv12I GWGCWC 1 cut(s) 155
BceAI ACGGC 2 cut(s) 250, 539
BclI TGATCA 1 cut(s) 433
BcoDI GTCTC 3 cut(s) 186, 235, 364
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 1 cut(s) 121
BfuAI ACCTGC 1 cut(s) 128
Bme18I GGWCC 3 cut(s) 659, 764, 773
BmeT110I CYCGRG 1 cut(s) 177
BmgT120I GGNCC 5 cut(s) 334, 335, 659, 764, 773
BmiI GGNNCC 4 cut(s) 336, 508, 766, 821
BmsI GCATC 1 cut(s) 173
BsaAI YACGTR 1 cut(s) 22
BsaBI GATNNNNATC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 1 cut(s) 178
BsaWI WCCGGW 2 cut(s) 247, 661
Bsc4I CCNNNNNNNGG 1 cut(s) 656
Bse8I GATNNNNATC 1 cut(s) 456
BseAI TCCGGA 1 cut(s) 661
BseDI CCNNGG 1 cut(s) 178
BseGI GGATG 3 cut(s) 188, 388, 412
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 1 cut(s) 656
BseMII CTCAG 1 cut(s) 22
BseSI GKGCMC 2 cut(s) 155, 338
BshFI GGCC 3 cut(s) 237, 336, 649
BshNI GGYRCC 1 cut(s) 506
BsiHKAI GWGCWC 1 cut(s) 155
BsiHKCI CYCGRG 1 cut(s) 177
BsiSI CCGG 2 cut(s) 248, 662
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 1 cut(s) 656
BsmAI GTCTC 3 cut(s) 186, 235, 364
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 3 cut(s) 237, 336, 649
Bso31I GGTCTC 1 cut(s) 235
BsoBI CYCGRG 1 cut(s) 177
Bsp120I GGGCCC 1 cut(s) 334
Bsp1286I GDGCHC 2 cut(s) 155, 338
Bsp13I TCCGGA 1 cut(s) 661
Bsp1407I TGTACA 1 cut(s) 532
Bsp143I GATC 1 cut(s) 433
BspACI CCGC 2 cut(s) 26, 762
BspANI GGCC 3 cut(s) 237, 336, 649
BspCNI CTCAG 1 cut(s) 21
BspEI TCCGGA 1 cut(s) 661
BspLI GGNNCC 4 cut(s) 336, 508, 766, 821
BspMAI CTGCAG 1 cut(s) 125
BspMI ACCTGC 1 cut(s) 128
BspT107I GGYRCC 1 cut(s) 506
BspTNI GGTCTC 1 cut(s) 235
BsrGI TGTACA 1 cut(s) 532
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 1 cut(s) 433
Bst4CI ACNGT 3 cut(s) 157, 514, 655
Bst6I CTCTTC 1 cut(s) 405
BstAUI TGTACA 1 cut(s) 532
BstBAI YACGTR 1 cut(s) 22
BstDEI CTNAG 1 cut(s) 8
BstF5I GGATG 3 cut(s) 188, 388, 412
BstHHI GCGC 1 cut(s) 301
BstKTI GATC 1 cut(s) 436
BstMAI GTCTC 3 cut(s) 186, 235, 364
BstMBI GATC 1 cut(s) 433
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstNSI RCATGY 2 cut(s) 377, 533
BstSFI CTRYAG 1 cut(s) 121
BstSLI GKGCMC 2 cut(s) 155, 338
BstSNI TACGTA 1 cut(s) 22
BstXI CCANNNNNNTGG 1 cut(s) 147
BsuRI GGCC 3 cut(s) 237, 336, 649
BtsCI GGATG 3 cut(s) 188, 388, 412
BtsIMutI CAGTG 1 cut(s) 510
BveI ACCTGC 1 cut(s) 128
CfoI GCGC 1 cut(s) 301
Cfr13I GGNCC 5 cut(s) 334, 335, 659, 764, 773
Csp6I GTAC 4 cut(s) 362, 533, 633, 726
CviAII CATG 4 cut(s) 374, 530, 713, 824
CviJI RGCY 9 cut(s) 14, 49, 89, 220, 237, 253, 336, 443, 649
CviKI_1 RGCY 9 cut(s) 14, 49, 89, 220, 237, 253, 336, 443, 649
CviQI GTAC 4 cut(s) 362, 533, 633, 726
DdeI CTNAG 1 cut(s) 8
DpnI GATC 1 cut(s) 435
DpnII GATC 1 cut(s) 433
DraI TTTAAA 1 cut(s) 696
DrdI GACNNNNNNGTC 1 cut(s) 771
DseDI GACNNNNNNGTC 1 cut(s) 771
EaeI YGGCCR 1 cut(s) 235
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
EciI GGCGGA 1 cut(s) 777
Eco105I TACGTA 1 cut(s) 22
Eco147I AGGCCT 1 cut(s) 649
Eco24I GRGCYC 1 cut(s) 338
Eco31I GGTCTC 1 cut(s) 235
Eco47I GGWCC 3 cut(s) 659, 764, 773
Eco88I CYCGRG 1 cut(s) 177
EcoO109I RGGNCCY 1 cut(s) 335
EcoRI GAATTC 1 cut(s) 470
EcoT38I GRGCYC 1 cut(s) 338
FaeI CATG 4 cut(s) 377, 533, 716, 827
FalI AAGNNNNNCTT 2 cut(s) 561, 593
FaqI GGGAC 1 cut(s) 371
FatI CATG 4 cut(s) 373, 529, 712, 823
FauNDI CATATG 1 cut(s) 778
FbaI TGATCA 1 cut(s) 433
FokI GGATG 3 cut(s) 195, 395, 419
FriOI GRGCYC 1 cut(s) 338
FspBI CTAG 1 cut(s) 317
GlaI GCGC 1 cut(s) 300
HaeIII GGCC 3 cut(s) 237, 336, 649
HapII CCGG 2 cut(s) 248, 662
HhaI GCGC 1 cut(s) 301
Hin1II CATG 4 cut(s) 377, 533, 716, 827
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HinfI GANTC 1 cut(s) 807
HpaII CCGG 2 cut(s) 248, 662
HphI GGTGA 1 cut(s) 802
Hpy166II GTNNAC 1 cut(s) 153
Hpy188I TCNGA 3 cut(s) 11, 819, 834
Hpy188III TCNNGA 6 cut(s) 115, 317, 497, 587, 662, 748
Hpy8I GTNNAC 1 cut(s) 153
HpyAV CCTTC 4 cut(s) 196, 497, 558, 844
HpyCH4III ACNGT 3 cut(s) 157, 514, 655
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 9 cut(s) 123, 153, 260, 290, 568, 602, 712, 782, 827
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpyF3I CTNAG 1 cut(s) 8
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 4 cut(s) 377, 533, 716, 827
HspAI GCGC 1 cut(s) 299
Kpn2I TCCGGA 1 cut(s) 661
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 1 cut(s) 433
LmnI GCTCC 2 cut(s) 9, 250
LweI GCATC 1 cut(s) 173
MaeI CTAG 1 cut(s) 317
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 2 cut(s) 514, 739
MalI GATC 1 cut(s) 435
MboI GATC 1 cut(s) 433
MboII GAAGA 4 cut(s) 220, 223, 422, 512
MhlI GDGCHC 2 cut(s) 155, 338
MluCI AATT 7 cut(s) 56, 427, 470, 480, 559, 801, 844
MroI TCCGGA 1 cut(s) 661
MseI TTAA 1 cut(s) 695
MslI CAYNNNNRTG 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 220
MspI CCGG 2 cut(s) 248, 662
MwoI GCNNNNNNNGC 1 cut(s) 296
NdeI CATATG 1 cut(s) 778
NdeII GATC 1 cut(s) 433
NlaIII CATG 4 cut(s) 377, 533, 716, 827
NlaIV GGNNCC 4 cut(s) 336, 508, 766, 821
NmuCI GTSAC 2 cut(s) 514, 739
NspI RCATGY 2 cut(s) 377, 533
PceI AGGCCT 1 cut(s) 649
PciI ACATGT 2 cut(s) 373, 529
PfeI GAWTC 1 cut(s) 807
Ppu21I YACGTR 1 cut(s) 22
PscI ACATGT 2 cut(s) 373, 529
PsiI TTATAA 1 cut(s) 327
PspN4I GGNNCC 4 cut(s) 336, 508, 766, 821
PspOMI GGGCCC 1 cut(s) 334
PspPI GGNCC 5 cut(s) 334, 335, 659, 764, 773
PsrI GAACNNNNNNTAC 2 cut(s) 267, 299
PstI CTGCAG 1 cut(s) 125
PvuII CAGCTG 1 cut(s) 220
RsaI GTAC 4 cut(s) 363, 534, 634, 727
RsaNI GTAC 4 cut(s) 362, 533, 633, 726
RseI CAYNNNNRTG 1 cut(s) 38
SaqAI TTAA 1 cut(s) 695
Sau3AI GATC 1 cut(s) 433
Sau96I GGNCC 5 cut(s) 334, 335, 659, 764, 773
SbfI CCTGCAGG 1 cut(s) 125
SdaI CCTGCAGG 1 cut(s) 125
SduI GDGCHC 2 cut(s) 155, 338
SfaNI GCATC 1 cut(s) 173
SfcI CTRYAG 1 cut(s) 121
SinI GGWCC 3 cut(s) 659, 764, 773
SmiMI CAYNNNNRTG 1 cut(s) 38
SnaBI TACGTA 1 cut(s) 22
Sse8387I CCTGCAGG 1 cut(s) 125
Sse9I AATT 7 cut(s) 56, 427, 470, 480, 559, 801, 844
SseBI AGGCCT 1 cut(s) 649
SsiI CCGC 2 cut(s) 26, 762
SspMI CTAG 1 cut(s) 317
StuI AGGCCT 1 cut(s) 649
TaaI ACNGT 3 cut(s) 157, 514, 655
TaiI ACGT 1 cut(s) 24
TasI AATT 7 cut(s) 56, 427, 470, 480, 559, 801, 844
TatI WGTACW 2 cut(s) 361, 532
TfiI GAWTC 1 cut(s) 807
Tru1I TTAA 1 cut(s) 695
Tru9I TTAA 1 cut(s) 695
TscAI CASTG 1 cut(s) 517
TseFI GTSAC 2 cut(s) 514, 739
Tsp45I GTSAC 2 cut(s) 514, 739
TspDTI ATGAA 6 cut(s) 423, 483, 492, 558, 588, 813
TspRI CASTG 1 cut(s) 517
VneI GTGCAC 1 cut(s) 151
VpaK11BI GGWCC 3 cut(s) 659, 764, 773
XapI RAATTY 5 cut(s) 56, 427, 470, 480, 559
XbaI TCTAGA 1 cut(s) 316
XceI RCATGY 2 cut(s) 377, 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 623
XspI CTAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.