Rmu_sc0000386.1_g000004

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000386.1
OG0000480
Physical Location & Seq
Reverse (-)
26827 .. 27731
905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000386.1_g000004.1.cds

Sequence Viewer

Length: 771 bp
atggaaggccacttttaccggtatgacctcagtgagcctttgttttcccaatacctcgaggctaaggatgccttcaaatcaatcgttccttactcagagatttcgataacggaggaagatgaagatggtgtcgctcctcgtcgtgagggagagcttccacaaatgattcctccaccttggaagaagcaaaagattgcagagccctctactaatgatggcccatctcgtgctctgcctactcaattagcttcgtattcttcttcatgtggaggttcgccctcctcgacgtccttgagggatattaccgctaacttttctccctcgaggacctttgcacccccgacaagcaccgaacttgatgctcaaagggccccttcactcccatcgggtccgacctcttccatgcaaccgcctcctcccaaagctctggaattgagattcaagaagcatgataactctttcatcatagctggagagtccttagactccaatggtttggcggagtcagccattgccggtgccttggcatcatcgaacgtgagatcgagtaggcctcccaagagcgctatggagttctatgatgacctatgctcctccgagctagatgctctcatggcctctcatcgttttttctgcagtcttgacgctcttcattccaggcagcaacaacgccttgaaaaggctgaccaagagctgaaatccaaggagcgaggaccgaaatcgcgaggtagcaaagttaaaaaaggatttggctttggtaactcaaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

256

Amino Acids

27.95

Weight (kDa)

6.46

Isoelectric Point (pI)

58.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000480)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04502
rosa_chinensis RchiOBHm_Chr2g0159401 RchiOBHm_Chr6g0254061
rosa_laevigata RLG00000014946 RLG00000035644 RLG00000035648
rosa_multiflora Rmu_co7989044.1_g000001 Rmu_co8071304.1_g000001 Rmu_co8335039.1_g000001 Rmu_co8433837.1_g000001 Rmu_co8463157.1_g000003 Rmu_co8495873.1_g000001 Rmu_sc0000030.1_g000034 Rmu_sc0000335.1_g000030 Rmu_sc0000386.1_g000004 Rmu_sc0000419.1_g000026 Rmu_sc0000536.1_g000025 Rmu_sc0000693.1_g000045 Rmu_sc0000950.1_g000029 Rmu_sc0000950.1_g000030 Rmu_sc0001524.1_g000001 Rmu_sc0001524.1_g000002 Rmu_sc0001716.1_g000027 Rmu_sc0001744.1_g000019 Rmu_sc0002162.1_g000032 Rmu_sc0002300.1_g000003 Rmu_sc0002341.1_g000002 Rmu_sc0002480.1_g000026 Rmu_sc0002759.1_g000071 Rmu_sc0002837.1_g000002 Rmu_sc0002837.1_g000003 Rmu_sc0002986.1_g000030 Rmu_sc0002986.1_g000031 Rmu_sc0003553.1_g000001 Rmu_sc0004192.1_g000015 Rmu_sc0004322.1_g000020 Rmu_sc0004755.1_g000032 Rmu_sc0004755.1_g000034 Rmu_sc0005163.1_g000005 Rmu_sc0005507.1_g000023 Rmu_sc0005599.1_g000013 Rmu_sc0005599.1_g000014 Rmu_sc0005599.1_g000015 Rmu_sc0005947.1_g000011 Rmu_sc0006031.1_g000027 Rmu_sc0006287.1_g000035 Rmu_sc0006318.1_g000009 Rmu_sc0007355.1_g000006 Rmu_sc0007355.1_g000007 Rmu_sc0007646.1_g000003 Rmu_sc0007953.1_g000003 Rmu_sc0008084.1_g000003 Rmu_sc0009135.1_g000005 Rmu_sc0009656.1_g000001 Rmu_sc0010871.1_g000011 Rmu_sc0010871.1_g000012 Rmu_sc0011938.1_g000002 Rmu_sc0013472.1_g000004 Rmu_sc0014143.1_g000001 Rmu_sc0015550.1_g000001 Rmu_sc0015855.1_g000002 Rmu_sc0017748.1_g000001 Rmu_sc0031144.1_g000001 Rmu_sc0032350.1_g000002 Rmu_sc0036344.1_g000003 Rmu_ssc0000042.1_g000002 Rmu_ssc0000255.1_g000043
rosa_roxburghii Rroxscaffold_1G00012780 Rroxscaffold_1G00031520 Rroxscaffold_1G00044950 Rroxscaffold_1G00044960 Rroxscaffold_2G00117760 Rroxscaffold_3G00224840 Rroxscaffold_3G00225760 Rroxscaffold_3G00235510 Rroxscaffold_3G00265420 Rroxscaffold_4G00315210 Rroxscaffold_4G00322430 Rroxscaffold_7G00165660 Rroxscaffold_7G00195200 Rroxscaffold_7G00211270
rosa_rugosa Rorug05G0252000 Rorug05G0548900
rosa_samantha Rh6AG065700 Rh6BG058500 Rh6CG058900 Rh6DG055700 Rh7DG180100
rosa_wichuraiana Rw6G005780 Rw6G033910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 290
AbsI CCTCGAGG 2 cut(s) 56, 322
AccB1I GGYRCC 1 cut(s) 518
AccII CGCG 1 cut(s) 724
AciI CCGC 3 cut(s) 306, 410, 500
AcyI GRCGYC 1 cut(s) 287
AfeI AGCGCT 1 cut(s) 565
AfiI CCNNNNNNNGG 1 cut(s) 679
AgeI ACCGGT 1 cut(s) 18
AgsI TTSAA 3 cut(s) 76, 442, 677
AjnI CCWGG 1 cut(s) 656
AluBI AGCT 6 cut(s) 154, 248, 425, 470, 601, 694
AluI AGCT 6 cut(s) 154, 248, 425, 470, 601, 694
Alw21I GWGCWC 1 cut(s) 232
Ama87I CYCGRG 2 cut(s) 56, 322
Aor51HI AGCGCT 1 cut(s) 565
AoxI GGCC 5 cut(s) 7, 217, 369, 551, 615
ApaI GGGCCC 1 cut(s) 373
ApeKI GCWGC 1 cut(s) 661
AsiGI ACCGGT 1 cut(s) 18
AspLEI GCGC 1 cut(s) 566
AspS9I GGNCC 6 cut(s) 218, 327, 369, 370, 389, 713
AvaI CYCGRG 2 cut(s) 56, 322
AvaII GGWCC 3 cut(s) 327, 389, 713
BaeGI GKGCMC 1 cut(s) 373
BanI GGYRCC 1 cut(s) 518
BanII GRGCYC 2 cut(s) 204, 373
BauI CACGAG 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 232
BbvI GCAGC 1 cut(s) 673
BccI CCATC 4 cut(s) 119, 209, 229, 391
BcgI CGANNNNNNTGC 4 cut(s) 341, 375, 587, 621
BciT130I CCWGG 1 cut(s) 658
BfaI CTAG 1 cut(s) 602
BfmI CTRYAG 1 cut(s) 634
BfoI RGCGCY 1 cut(s) 567
BisI GCNGC 1 cut(s) 662
BlsI GCNGC 1 cut(s) 663
Bme1390I CCNGG 1 cut(s) 658
Bme18I GGWCC 3 cut(s) 327, 389, 713
BmeT110I CYCGRG 2 cut(s) 56, 322
BmgT120I GGNCC 6 cut(s) 218, 327, 369, 370, 389, 713
BmiI GGNNCC 4 cut(s) 371, 372, 390, 520
BmrFI CCNGG 1 cut(s) 658
BmsI GCATC 4 cut(s) 58, 349, 536, 595
BplI GAGNNNNNCTC 2 cut(s) 538, 570
BpmI CTGGAG 1 cut(s) 492
Bpu10I CCTNAGC 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 313
BsaHI GRCGYC 1 cut(s) 287
BsaJI CCNNGG 3 cut(s) 176, 522, 702
BsaWI WCCGGW 1 cut(s) 18
BsaXI ACNNNNNCTCC 2 cut(s) 575, 605
Bsc4I CCNNNNNNNGG 1 cut(s) 679
Bse118I RCCGGY 2 cut(s) 18, 515
Bse3DI GCAATG 1 cut(s) 510
BseBI CCWGG 1 cut(s) 658
BseDI CCNNGG 3 cut(s) 176, 522, 702
BseGI GGATG 1 cut(s) 73
BseLI CCNNNNNNNGG 1 cut(s) 679
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 2 cut(s) 43, 108
BseRI GAGGAG 4 cut(s) 126, 271, 405, 583
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 1 cut(s) 673
Bsh1236I CGCG 1 cut(s) 724
BshFI GGCC 5 cut(s) 9, 219, 371, 553, 617
BshNI GGYRCC 1 cut(s) 518
BshTI ACCGGT 1 cut(s) 18
BsiHKAI GWGCWC 1 cut(s) 232
BsiHKCI CYCGRG 2 cut(s) 56, 322
BsiSI CCGG 2 cut(s) 19, 516
BslI CCNNNNNNNGG 1 cut(s) 679
BsnI GGCC 5 cut(s) 9, 219, 371, 553, 617
BsoBI CYCGRG 2 cut(s) 56, 322
Bsp120I GGGCCC 1 cut(s) 369
Bsp1286I GDGCHC 3 cut(s) 204, 232, 373
Bsp143I GATC 1 cut(s) 542
Bsp68I TCGCGA 1 cut(s) 724
BspACI CCGC 3 cut(s) 306, 410, 500
BspANI GGCC 5 cut(s) 9, 219, 371, 553, 617
BspCNI CTCAG 2 cut(s) 42, 107
BspFNI CGCG 1 cut(s) 724
BspLI GGNNCC 4 cut(s) 371, 372, 390, 520
BspMAI CTGCAG 1 cut(s) 638
BspQI GCTCTTC 1 cut(s) 654
BspT107I GGYRCC 1 cut(s) 518
BsrDI GCAATG 1 cut(s) 510
BsrFI RCCGGY 2 cut(s) 18, 515
BssAI RCCGGY 2 cut(s) 18, 515
BssECI CCNNGG 3 cut(s) 176, 522, 702
BssMI GATC 1 cut(s) 542
BssNI GRCGYC 1 cut(s) 287
BssSI CACGAG 1 cut(s) 225
BssT1I CCWWGG 3 cut(s) 176, 522, 702
Bst2BI CACGAG 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 658
Bst6I CTCTTC 2 cut(s) 403, 654
BstACI GRCGYC 1 cut(s) 287
BstDEI CTNAG 4 cut(s) 29, 63, 94, 481
BstENI CCTNNNNNAGG 1 cut(s) 677
BstF5I GGATG 1 cut(s) 73
BstFNI CGCG 1 cut(s) 724
BstH2I RGCGCY 1 cut(s) 567
BstHHI GCGC 1 cut(s) 566
BstKTI GATC 1 cut(s) 545
BstMBI GATC 1 cut(s) 542
BstMWI GCNNNNNNNGC 4 cut(s) 68, 368, 506, 614
BstNI CCWGG 1 cut(s) 658
BstSCI CCNGG 1 cut(s) 656
BstSFI CTRYAG 1 cut(s) 634
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 1 cut(s) 724
BstV1I GCAGC 1 cut(s) 673
BstXI CCANNNNNNTGG 2 cut(s) 427, 496
BsuRI GGCC 5 cut(s) 9, 219, 371, 553, 617
BtsCI GGATG 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 37
BtuMI TCGCGA 1 cut(s) 724
CfoI GCGC 1 cut(s) 566
Cfr10I RCCGGY 2 cut(s) 18, 515
Cfr13I GGNCC 6 cut(s) 218, 327, 369, 370, 389, 713
CseI GACGC 1 cut(s) 653
CspAI ACCGGT 1 cut(s) 18
CviAII CATG 4 cut(s) 264, 403, 449, 613
DdeI CTNAG 4 cut(s) 29, 63, 94, 481
DpnI GATC 1 cut(s) 544
DpnII GATC 1 cut(s) 542
Eam1104I CTCTTC 2 cut(s) 403, 654
EarI CTCTTC 2 cut(s) 403, 654
EciI GGCGGA 1 cut(s) 515
Eco130I CCWWGG 3 cut(s) 176, 522, 702
Eco147I AGGCCT 1 cut(s) 553
Eco24I GRGCYC 2 cut(s) 204, 373
Eco47I GGWCC 3 cut(s) 327, 389, 713
Eco47III AGCGCT 1 cut(s) 565
Eco88I CYCGRG 2 cut(s) 56, 322
EcoNI CCTNNNNNAGG 1 cut(s) 677
EcoO109I RGGNCCY 3 cut(s) 327, 369, 370
EcoRII CCWGG 1 cut(s) 656
EcoT14I CCWWGG 3 cut(s) 176, 522, 702
EcoT38I GRGCYC 2 cut(s) 204, 373
ErhI CCWWGG 3 cut(s) 176, 522, 702
FaeI CATG 4 cut(s) 267, 406, 452, 616
FaiI YATR 9 cut(s) 24, 265, 404, 450, 467, 569, 579, 589, 614
FalI AAGNNNNNCTT 4 cut(s) 56, 88, 358, 390
FatI CATG 4 cut(s) 263, 402, 448, 612
Fnu4HI GCNGC 1 cut(s) 662
FokI GGATG 1 cut(s) 80
FriOI GRGCYC 2 cut(s) 204, 373
Fsp4HI GCNGC 1 cut(s) 662
FspBI CTAG 1 cut(s) 602
GlaI GCGC 1 cut(s) 565
GluI GCNGC 1 cut(s) 662
GsuI CTGGAG 1 cut(s) 492
HaeII RGCGCY 1 cut(s) 567
HaeIII GGCC 5 cut(s) 9, 219, 371, 553, 617
HapII CCGG 2 cut(s) 19, 516
HgaI GACGC 1 cut(s) 653
HhaI GCGC 1 cut(s) 566
Hin1I GRCGYC 1 cut(s) 287
Hin1II CATG 4 cut(s) 267, 406, 452, 616
Hin6I GCGC 1 cut(s) 564
HinP1I GCGC 1 cut(s) 564
HinfI GANTC 5 cut(s) 166, 438, 476, 485, 503
HpaII CCGG 2 cut(s) 19, 516
Hpy188I TCNGA 3 cut(s) 97, 393, 598
Hpy188III TCNNGA 5 cut(s) 143, 428, 442, 641, 723
Hpy99I CGWCG 2 cut(s) 144, 289
HpyAV CCTTC 2 cut(s) 82, 384
HpyCH4IV ACGT 2 cut(s) 287, 537
HpyCH4V TGCA 4 cut(s) 197, 335, 406, 636
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 368, 506, 614
HpyF3I CTNAG 4 cut(s) 29, 63, 94, 481
HpySE526I ACGT 2 cut(s) 287, 537
Hsp92I GRCGYC 1 cut(s) 287
Hsp92II CATG 4 cut(s) 267, 406, 452, 616
HspAI GCGC 1 cut(s) 564
Kzo9I GATC 1 cut(s) 542
LguI GCTCTTC 1 cut(s) 654
LmnI GCTCC 3 cut(s) 139, 596, 706
LpnPI CCDG 6 cut(s) 32, 413, 456, 529, 643, 670
Lsp1109I GCAGC 1 cut(s) 673
LweI GCATC 4 cut(s) 58, 349, 536, 595
MaeI CTAG 1 cut(s) 602
MaeII ACGT 2 cut(s) 287, 537
MaeIII GTNAC 1 cut(s) 758
MalI GATC 1 cut(s) 544
MboI GATC 1 cut(s) 542
MboII GAAGA 7 cut(s) 128, 134, 193, 249, 252, 390, 641
MhlI GDGCHC 3 cut(s) 204, 232, 373
MluCI AATT 2 cut(s) 242, 431
MlyI GAGTC 3 cut(s) 479, 485, 512
MmeI TCCRAC 1 cut(s) 416
MseI TTAA 1 cut(s) 738
MspI CCGG 2 cut(s) 19, 516
MspR9I CCNGG 1 cut(s) 658
MvaI CCWGG 1 cut(s) 658
MvnI CGCG 1 cut(s) 724
MwoI GCNNNNNNNGC 4 cut(s) 68, 368, 506, 614
NdeII GATC 1 cut(s) 542
NlaIII CATG 4 cut(s) 267, 406, 452, 616
NlaIV GGNNCC 4 cut(s) 371, 372, 390, 520
NruI TCGCGA 1 cut(s) 724
PaeR7I CTCGAG 2 cut(s) 56, 322
PceI AGGCCT 1 cut(s) 553
PciSI GCTCTTC 1 cut(s) 654
PcsI WCGNNNNNNNCGW 1 cut(s) 281
PfeI GAWTC 2 cut(s) 166, 438
PinAI ACCGGT 1 cut(s) 18
PkrI GCNGC 1 cut(s) 663
PleI GAGTC 3 cut(s) 479, 484, 511
PpsI GAGTC 3 cut(s) 479, 484, 511
PpuMI RGGWCCY 1 cut(s) 327
Psp5II RGGWCCY 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 656
PspGI CCWGG 1 cut(s) 656
PspN4I GGNNCC 4 cut(s) 371, 372, 390, 520
PspOMI GGGCCC 1 cut(s) 369
PspPI GGNCC 6 cut(s) 218, 327, 369, 370, 389, 713
PspPPI RGGWCCY 1 cut(s) 327
PspXI VCTCGAGB 2 cut(s) 56, 322
PstI CTGCAG 1 cut(s) 638
RruI TCGCGA 1 cut(s) 724
SapI GCTCTTC 1 cut(s) 654
SaqAI TTAA 1 cut(s) 738
SatI GCNGC 1 cut(s) 662
Sau3AI GATC 1 cut(s) 542
Sau96I GGNCC 6 cut(s) 218, 327, 369, 370, 389, 713
SchI GAGTC 3 cut(s) 479, 485, 512
ScrFI CCNGG 1 cut(s) 658
SduI GDGCHC 3 cut(s) 204, 232, 373
SfaNI GCATC 4 cut(s) 58, 349, 536, 595
SfcI CTRYAG 1 cut(s) 634
Sfr274I CTCGAG 2 cut(s) 56, 322
SinI GGWCC 3 cut(s) 327, 389, 713
SlaI CTCGAG 2 cut(s) 56, 322
SmlI CTYRAG 3 cut(s) 56, 292, 322
SmoI CTYRAG 3 cut(s) 56, 292, 322
Sse9I AATT 2 cut(s) 242, 431
SseBI AGGCCT 1 cut(s) 553
SsiI CCGC 3 cut(s) 306, 410, 500
SspMI CTAG 1 cut(s) 602
StuI AGGCCT 1 cut(s) 553
StyD4I CCNGG 1 cut(s) 656
StyI CCWWGG 3 cut(s) 176, 522, 702
TaiI ACGT 2 cut(s) 290, 540
TaqI TCGA 6 cut(s) 57, 104, 284, 323, 533, 545
TaqII GACCGA 1 cut(s) 730
TasI AATT 2 cut(s) 242, 431
TfiI GAWTC 2 cut(s) 166, 438
Tru1I TTAA 1 cut(s) 738
Tru9I TTAA 1 cut(s) 738
TscAI CASTG 1 cut(s) 37
TseI GCWGC 1 cut(s) 661
TspDTI ATGAA 4 cut(s) 135, 252, 451, 641
TspGWI ACGGA 1 cut(s) 125
TspRI CASTG 1 cut(s) 37
VpaK11BI GGWCC 3 cut(s) 327, 389, 713
XagI CCTNNNNNAGG 1 cut(s) 677
XcmI CCANNNNNNNNNTGG 1 cut(s) 565
XhoI CTCGAG 2 cut(s) 56, 322
XspI CTAG 1 cut(s) 602
ZraI GACGTC 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.