Rmu_sc0002300.1_g000003

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002300.1
OG0000480
Physical Location & Seq
Reverse (-)
32251 .. 37463
5213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002300.1_g000003.1.cds

Sequence Viewer

Length: 1140 bp
atgtggctcttggtttggtttggttttggctacccaagttccaccggatccaatgcaagggttgcggaaggctcaatggtgttacctacacgaactgctttgagtcggaaaggatactctggagacgtagggtgtctgctatgcccgagtcacactgaagatgccatgcatttatttatacattgcccatatgcagttagtgtgtgggctgctgcaaacatagttgtggacacttcttttctggatttcaaggagtttttgttagctttgaagcggagactccttttcaccgtcggtgtgaaagaggaggaggggtacctgcaaaagaccctccgatgcctaagtcagtatttttctgagtcggcttataagatttctcaagcgaagttcgaccttccccctagtcgcctacaatggctagagactcatcttatcgggcagggatggtatccctctaagttcacaaccctcgagaattttcttggcatggtgtccaatcgtccttctgttttgtttggctgctaccgaacgcatcgaaaggctgaaaatgctcctgacagcgacaaccccttgcatgttccctccatcgactcgtctcgtggtgccctgccgccctctccctcacgagggggctcacttccccaaagaactgccccaggcgaagctgctgatcctgctcttcagagatcctcttcgtctcaagaggatccaaccgctttcatgccgtcgccgccacccaaggctctcttggcatctcatcgcctcttgcacgacattgacactttgcataccaaaccacaacagaaactcaacgaggcgaattgtaaactcgagtttaaagagaaagagctcgacgatcagatgaggacaatggtagaattggcctctgtccttgaggacaagaaatgcgagataacaaggctgaagaatgacctggccttagtaactcgaagcaaagaagaggagttccaaaaaggtgctgctcagatgaagcgcgtgtcaaccaaatacttccgacttgggtggaatcaagctctgactgaagaagacaaaaggcactacgtaggcgctgatgagttcgaccatttttatgggtttgatctatgggttattctcccatccaacctcaagctcgtctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

379

Amino Acids

42.72

Weight (kDa)

7.16

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000480)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04502
rosa_chinensis RchiOBHm_Chr2g0159401 RchiOBHm_Chr6g0254061
rosa_laevigata RLG00000014946 RLG00000035644 RLG00000035648
rosa_multiflora Rmu_co7989044.1_g000001 Rmu_co8071304.1_g000001 Rmu_co8335039.1_g000001 Rmu_co8433837.1_g000001 Rmu_co8463157.1_g000003 Rmu_co8495873.1_g000001 Rmu_sc0000030.1_g000034 Rmu_sc0000335.1_g000030 Rmu_sc0000386.1_g000004 Rmu_sc0000419.1_g000026 Rmu_sc0000536.1_g000025 Rmu_sc0000693.1_g000045 Rmu_sc0000950.1_g000029 Rmu_sc0000950.1_g000030 Rmu_sc0001524.1_g000001 Rmu_sc0001524.1_g000002 Rmu_sc0001716.1_g000027 Rmu_sc0001744.1_g000019 Rmu_sc0002162.1_g000032 Rmu_sc0002300.1_g000003 Rmu_sc0002341.1_g000002 Rmu_sc0002480.1_g000026 Rmu_sc0002759.1_g000071 Rmu_sc0002837.1_g000002 Rmu_sc0002837.1_g000003 Rmu_sc0002986.1_g000030 Rmu_sc0002986.1_g000031 Rmu_sc0003553.1_g000001 Rmu_sc0004192.1_g000015 Rmu_sc0004322.1_g000020 Rmu_sc0004755.1_g000032 Rmu_sc0004755.1_g000034 Rmu_sc0005163.1_g000005 Rmu_sc0005507.1_g000023 Rmu_sc0005599.1_g000013 Rmu_sc0005599.1_g000014 Rmu_sc0005599.1_g000015 Rmu_sc0005947.1_g000011 Rmu_sc0006031.1_g000027 Rmu_sc0006287.1_g000035 Rmu_sc0006318.1_g000009 Rmu_sc0007355.1_g000006 Rmu_sc0007355.1_g000007 Rmu_sc0007646.1_g000003 Rmu_sc0007953.1_g000003 Rmu_sc0008084.1_g000003 Rmu_sc0009135.1_g000005 Rmu_sc0009656.1_g000001 Rmu_sc0010871.1_g000011 Rmu_sc0010871.1_g000012 Rmu_sc0011938.1_g000002 Rmu_sc0013472.1_g000004 Rmu_sc0014143.1_g000001 Rmu_sc0015550.1_g000001 Rmu_sc0015855.1_g000002 Rmu_sc0017748.1_g000001 Rmu_sc0031144.1_g000001 Rmu_sc0032350.1_g000002 Rmu_sc0036344.1_g000003 Rmu_ssc0000042.1_g000002 Rmu_ssc0000255.1_g000043
rosa_roxburghii Rroxscaffold_1G00012780 Rroxscaffold_1G00031520 Rroxscaffold_1G00044950 Rroxscaffold_1G00044960 Rroxscaffold_2G00117760 Rroxscaffold_3G00224840 Rroxscaffold_3G00225760 Rroxscaffold_3G00235510 Rroxscaffold_3G00265420 Rroxscaffold_4G00315210 Rroxscaffold_4G00322430 Rroxscaffold_7G00165660 Rroxscaffold_7G00195200 Rroxscaffold_7G00211270
rosa_rugosa Rorug05G0252000 Rorug05G0548900
rosa_samantha Rh6AG065700 Rh6BG058500 Rh6CG058900 Rh6DG055700 Rh7DG180100
rosa_wichuraiana Rw6G005780 Rw6G033910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
Acc36I ACCTGC 1 cut(s) 327
Acc65I GGTACC 1 cut(s) 315
AccB1I GGYRCC 2 cut(s) 315, 602
AccII CGCG 1 cut(s) 996
AciI CCGC 5 cut(s) 65, 274, 611, 714, 731
AclWI GGATC 6 cut(s) 42, 55, 665, 681, 701, 714
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 4 cut(s) 177, 665, 944, 1062
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 3 cut(s) 57, 626, 627
AgsI TTSAA 2 cut(s) 250, 271
AjnI CCWGG 2 cut(s) 655, 933
AluBI AGCT 5 cut(s) 266, 665, 850, 1034, 1132
AluI AGCT 5 cut(s) 266, 665, 850, 1034, 1132
Alw21I GWGCWC 1 cut(s) 852
Alw26I GTCTC 5 cut(s) 117, 271, 416, 600, 702
AlwI GGATC 6 cut(s) 42, 55, 665, 681, 701, 714
Ama87I CYCGRG 3 cut(s) 145, 470, 830
AoxI GGCC 2 cut(s) 882, 936
ApeKI GCWGC 5 cut(s) 209, 212, 519, 665, 980
ApoI RAATTY 1 cut(s) 475
Asp718I GGTACC 1 cut(s) 315
AspLEI GCGC 2 cut(s) 996, 1070
AsuHPI GGTGA 1 cut(s) 280
AvaI CYCGRG 3 cut(s) 145, 470, 830
BaeGI GKGCMC 1 cut(s) 607
BamHI GGATCC 2 cut(s) 47, 706
BanI GGYRCC 2 cut(s) 315, 602
BanII GRGCYC 2 cut(s) 635, 852
BauI CACGAG 2 cut(s) 597, 624
BbsI GAAGAC 1 cut(s) 1053
Bbv12I GWGCWC 1 cut(s) 852
BbvI GCAGC 5 cut(s) 196, 199, 506, 652, 967
BccI CCATC 3 cut(s) 438, 593, 1126
BceAI ACGGC 1 cut(s) 709
BciT130I CCWGG 2 cut(s) 657, 935
BciVI GTATCC 2 cut(s) 107, 459
BcoDI GTCTC 5 cut(s) 117, 271, 416, 600, 702
BfaI CTAG 2 cut(s) 402, 419
BfoI RGCGCY 1 cut(s) 1071
BfuAI ACCTGC 1 cut(s) 327
BfuI GTATCC 2 cut(s) 107, 459
BisI GCNGC 7 cut(s) 210, 213, 520, 611, 666, 731, 981
BlsI GCNGC 7 cut(s) 211, 214, 521, 612, 667, 732, 982
Bme1390I CCNGG 2 cut(s) 657, 935
BmeT110I CYCGRG 3 cut(s) 145, 470, 830
BmiI GGNNCC 4 cut(s) 49, 317, 604, 708
BmrFI CCNGG 2 cut(s) 657, 935
BmsI GCATC 4 cut(s) 151, 326, 541, 761
BpiI GAAGAC 1 cut(s) 1053
BpmI CTGGAG 1 cut(s) 141
BpuEI CTTGAG 4 cut(s) 363, 684, 914, 1112
BsaAI YACGTR 1 cut(s) 1063
BsaJI CCNNGG 2 cut(s) 655, 738
BsaWI WCCGGW 1 cut(s) 44
BsaXI ACNNNNNCTCC 4 cut(s) 245, 275, 299, 329
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 626, 627
Bse3DI GCAATG 1 cut(s) 181
BseBI CCWGG 2 cut(s) 657, 935
BseDI CCNNGG 2 cut(s) 655, 738
BseGI GGATG 2 cut(s) 449, 1118
BseLI CCNNNNNNNGG 3 cut(s) 57, 626, 627
BseMI GCAATG 1 cut(s) 181
BseMII CTCAG 2 cut(s) 348, 998
BseRI GAGGAG 3 cut(s) 320, 323, 977
BseSI GKGCMC 1 cut(s) 607
BseXI GCAGC 5 cut(s) 196, 199, 506, 652, 967
Bsh1236I CGCG 1 cut(s) 996
BshFI GGCC 2 cut(s) 884, 938
BshNI GGYRCC 2 cut(s) 315, 602
BsiHKAI GWGCWC 1 cut(s) 852
BsiHKCI CYCGRG 3 cut(s) 145, 470, 830
BsiSI CCGG 1 cut(s) 45
BslI CCNNNNNNNGG 3 cut(s) 57, 626, 627
BsmAI GTCTC 5 cut(s) 117, 271, 416, 600, 702
BsmBI CGTCTC 3 cut(s) 117, 600, 702
BsnI GGCC 2 cut(s) 884, 938
BsoBI CYCGRG 3 cut(s) 145, 470, 830
Bsp1286I GDGCHC 3 cut(s) 607, 635, 852
Bsp143I GATC 6 cut(s) 47, 670, 686, 706, 856, 1099
BspACI CCGC 5 cut(s) 65, 274, 611, 714, 731
BspANI GGCC 2 cut(s) 884, 938
BspCNI CTCAG 2 cut(s) 349, 997
BspFNI CGCG 1 cut(s) 996
BspLI GGNNCC 4 cut(s) 49, 317, 604, 708
BspMI ACCTGC 1 cut(s) 327
BspPI GGATC 6 cut(s) 42, 55, 665, 681, 701, 714
BspQI GCTCTTC 1 cut(s) 684
BspT107I GGYRCC 2 cut(s) 315, 602
BsrDI GCAATG 1 cut(s) 181
BssECI CCNNGG 2 cut(s) 655, 738
BssMI GATC 6 cut(s) 47, 670, 686, 706, 856, 1099
BssSI CACGAG 2 cut(s) 597, 624
BssT1I CCWWGG 1 cut(s) 738
Bst2BI CACGAG 2 cut(s) 597, 624
Bst2UI CCWGG 2 cut(s) 657, 935
Bst4CI ACNGT 1 cut(s) 292
Bst6I CTCTTC 3 cut(s) 684, 697, 954
BstAPI GCANNNNNTGC 1 cut(s) 62
BstBAI YACGTR 1 cut(s) 1063
BstDEI CTNAG 5 cut(s) 341, 357, 456, 940, 984
BstF5I GGATG 2 cut(s) 449, 1118
BstFNI CGCG 1 cut(s) 996
BstH2I RGCGCY 1 cut(s) 1071
BstHHI GCGC 2 cut(s) 996, 1070
BstKTI GATC 6 cut(s) 50, 673, 689, 709, 859, 1102
BstMAI GTCTC 5 cut(s) 117, 271, 416, 600, 702
BstMBI GATC 6 cut(s) 47, 670, 686, 706, 856, 1099
BstMWI GCNNNNNNNGC 5 cut(s) 62, 548, 674, 730, 749
BstNI CCWGG 2 cut(s) 657, 935
BstNSI RCATGY 1 cut(s) 578
BstSCI CCNGG 2 cut(s) 655, 933
BstSLI GKGCMC 1 cut(s) 607
BstSNI TACGTA 1 cut(s) 1063
BstUI CGCG 1 cut(s) 996
BstV1I GCAGC 5 cut(s) 196, 199, 506, 652, 967
BstV2I GAAGAC 1 cut(s) 1053
BstX2I RGATCY 3 cut(s) 47, 686, 706
BstXI CCANNNNNNTGG 1 cut(s) 1091
BstYI RGATCY 3 cut(s) 47, 686, 706
BsuI GTATCC 2 cut(s) 107, 459
BsuRI GGCC 2 cut(s) 884, 938
BtgZI GCGATG 1 cut(s) 743
BtsCI GGATG 2 cut(s) 449, 1118
BtsIMutI CAGTG 1 cut(s) 153
BveI ACCTGC 1 cut(s) 327
CfoI GCGC 2 cut(s) 996, 1070
Csp6I GTAC 1 cut(s) 316
CviAII CATG 4 cut(s) 166, 487, 575, 721
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 5 cut(s) 341, 357, 456, 940, 984
DpnI GATC 6 cut(s) 49, 672, 688, 708, 858, 1101
DpnII GATC 6 cut(s) 47, 670, 686, 706, 856, 1099
DraI TTTAAA 1 cut(s) 838
Eam1104I CTCTTC 3 cut(s) 684, 697, 954
EarI CTCTTC 3 cut(s) 684, 697, 954
Ecl136II GAGCTC 1 cut(s) 850
Eco105I TACGTA 1 cut(s) 1063
Eco130I CCWWGG 1 cut(s) 738
Eco24I GRGCYC 2 cut(s) 635, 852
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 4 cut(s) 177, 665, 944, 1062
Eco88I CYCGRG 3 cut(s) 145, 470, 830
EcoICRI GAGCTC 1 cut(s) 850
EcoRII CCWGG 2 cut(s) 655, 933
EcoT14I CCWWGG 1 cut(s) 738
EcoT22I ATGCAT 1 cut(s) 171
EcoT38I GRGCYC 2 cut(s) 635, 852
ErhI CCWWGG 1 cut(s) 738
Esp3I CGTCTC 3 cut(s) 117, 600, 702
FaeI CATG 4 cut(s) 169, 490, 578, 724
FalI AAGNNNNNCTT 2 cut(s) 731, 763
FatI CATG 4 cut(s) 165, 486, 574, 720
FauNDI CATATG 1 cut(s) 190
Fnu4HI GCNGC 7 cut(s) 210, 213, 520, 611, 666, 731, 981
FokI GGATG 2 cut(s) 456, 1105
FriOI GRGCYC 2 cut(s) 635, 852
Fsp4HI GCNGC 7 cut(s) 210, 213, 520, 611, 666, 731, 981
FspBI CTAG 2 cut(s) 402, 419
GlaI GCGC 2 cut(s) 995, 1069
GluI GCNGC 7 cut(s) 210, 213, 520, 611, 666, 731, 981
GsuI CTGGAG 1 cut(s) 141
HaeII RGCGCY 1 cut(s) 1071
HaeIII GGCC 2 cut(s) 884, 938
HapII CCGG 1 cut(s) 45
HhaI GCGC 2 cut(s) 996, 1070
Hin1II CATG 4 cut(s) 169, 490, 578, 724
Hin6I GCGC 2 cut(s) 994, 1068
HinP1I GCGC 2 cut(s) 994, 1068
HincII GTYRAC 1 cut(s) 1002
HindII GTYRAC 1 cut(s) 1002
HinfI GANTC 7 cut(s) 103, 148, 279, 359, 424, 590, 1027
HpaII CCGG 1 cut(s) 45
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 4 cut(s) 229, 462, 827, 1002
Hpy188I TCNGA 9 cut(s) 108, 335, 358, 684, 861, 987, 1016, 1038, 1139
Hpy188III TCNNGA 6 cut(s) 120, 242, 472, 554, 624, 701
Hpy8I GTNNAC 4 cut(s) 229, 462, 827, 1002
Hpy99I CGWCG 3 cut(s) 296, 730, 857
HpyAV CCTTC 3 cut(s) 62, 404, 513
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 2 cut(s) 126, 1062
HpyCH4V TGCA 8 cut(s) 56, 169, 194, 215, 322, 574, 769, 787
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 548, 674, 730, 749
HpyF3I CTNAG 5 cut(s) 341, 357, 456, 940, 984
HpySE526I ACGT 2 cut(s) 126, 1062
Hsp92II CATG 4 cut(s) 169, 490, 578, 724
HspAI GCGC 2 cut(s) 994, 1068
KpnI GGTACC 1 cut(s) 319
Kzo9I GATC 6 cut(s) 47, 670, 686, 706, 856, 1099
LguI GCTCTTC 1 cut(s) 684
LmnI GCTCC 1 cut(s) 556
Lsp1109I GCAGC 5 cut(s) 196, 199, 506, 652, 967
LweI GCATC 4 cut(s) 151, 326, 541, 761
MaeI CTAG 2 cut(s) 402, 419
MaeII ACGT 2 cut(s) 126, 1062
MaeIII GTNAC 3 cut(s) 81, 149, 943
MalI GATC 6 cut(s) 49, 672, 688, 708, 858, 1101
MboI GATC 6 cut(s) 47, 670, 686, 706, 856, 1099
MboII GAAGA 7 cut(s) 170, 671, 684, 937, 971, 1055, 1058
MflI RGATCY 3 cut(s) 47, 686, 706
MhlI GDGCHC 3 cut(s) 607, 635, 852
MluCI AATT 3 cut(s) 475, 820, 878
MlyI GAGTC 6 cut(s) 112, 157, 273, 368, 418, 584
MmeI TCCRAC 3 cut(s) 86, 734, 1039
Mph1103I ATGCAT 1 cut(s) 171
MseI TTAA 1 cut(s) 837
MslI CAYNNNNRTG 2 cut(s) 224, 1089
MspI CCGG 1 cut(s) 45
MspR9I CCNGG 2 cut(s) 657, 935
MvaI CCWGG 2 cut(s) 657, 935
MvnI CGCG 1 cut(s) 996
MwoI GCNNNNNNNGC 5 cut(s) 62, 548, 674, 730, 749
NdeI CATATG 1 cut(s) 190
NdeII GATC 6 cut(s) 47, 670, 686, 706, 856, 1099
NlaIII CATG 4 cut(s) 169, 490, 578, 724
NlaIV GGNNCC 4 cut(s) 49, 317, 604, 708
NmuCI GTSAC 1 cut(s) 149
NsiI ATGCAT 1 cut(s) 171
NspI RCATGY 1 cut(s) 578
PaeR7I CTCGAG 2 cut(s) 470, 830
PciSI GCTCTTC 1 cut(s) 684
PfeI GAWTC 1 cut(s) 1027
PkrI GCNGC 7 cut(s) 211, 214, 521, 612, 667, 732, 982
PleI GAGTC 6 cut(s) 111, 156, 273, 367, 418, 584
PpsI GAGTC 6 cut(s) 111, 156, 273, 367, 418, 584
Ppu21I YACGTR 1 cut(s) 1063
PsiI TTATAA 1 cut(s) 369
Psp124BI GAGCTC 1 cut(s) 852
Psp6I CCWGG 2 cut(s) 655, 933
PspGI CCWGG 2 cut(s) 655, 933
PspN4I GGNNCC 4 cut(s) 49, 317, 604, 708
PspXI VCTCGAGB 1 cut(s) 830
PsuI RGATCY 3 cut(s) 47, 686, 706
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
RseI CAYNNNNRTG 2 cut(s) 224, 1089
SacI GAGCTC 1 cut(s) 852
SapI GCTCTTC 1 cut(s) 684
SaqAI TTAA 1 cut(s) 837
SatI GCNGC 7 cut(s) 210, 213, 520, 611, 666, 731, 981
Sau3AI GATC 6 cut(s) 47, 670, 686, 706, 856, 1099
SchI GAGTC 6 cut(s) 112, 157, 273, 368, 418, 584
ScrFI CCNGG 2 cut(s) 657, 935
SduI GDGCHC 3 cut(s) 607, 635, 852
SfaNI GCATC 4 cut(s) 151, 326, 541, 761
Sfr274I CTCGAG 2 cut(s) 470, 830
SlaI CTCGAG 2 cut(s) 470, 830
SmiMI CAYNNNNRTG 2 cut(s) 224, 1089
SmlI CTYRAG 6 cut(s) 378, 470, 699, 830, 893, 1127
SmoI CTYRAG 6 cut(s) 378, 470, 699, 830, 893, 1127
SnaBI TACGTA 1 cut(s) 1063
Sse9I AATT 3 cut(s) 475, 820, 878
SsiI CCGC 5 cut(s) 65, 274, 611, 714, 731
SspMI CTAG 2 cut(s) 402, 419
SstI GAGCTC 1 cut(s) 852
StyD4I CCNGG 2 cut(s) 655, 933
StyI CCWWGG 1 cut(s) 738
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 2 cut(s) 129, 1065
TaqI TCGA 8 cut(s) 390, 471, 535, 588, 831, 852, 949, 1080
TasI AATT 3 cut(s) 475, 820, 878
TauI GCSGC 2 cut(s) 613, 733
TfiI GAWTC 1 cut(s) 1027
Tru1I TTAA 1 cut(s) 837
Tru9I TTAA 1 cut(s) 837
TscAI CASTG 1 cut(s) 160
TseFI GTSAC 1 cut(s) 149
TseI GCWGC 5 cut(s) 209, 212, 519, 665, 980
Tsp45I GTSAC 1 cut(s) 149
TspDTI ATGAA 2 cut(s) 709, 1004
TspRI CASTG 1 cut(s) 160
XapI RAATTY 1 cut(s) 475
XceI RCATGY 1 cut(s) 578
XcmI CCANNNNNNNNNTGG 1 cut(s) 745
XhoI CTCGAG 2 cut(s) 470, 830
XspI CTAG 2 cut(s) 402, 419
Zsp2I ATGCAT 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.