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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 2122 |
| Acc16I | TGCGCA | 1 cut(s) | 1993 |
| AccB1I | GGYRCC | 3 cut(s) | 965, 979, 1289 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 75 |
| AccBSI | CCGCTC | 3 cut(s) | 476, 722, 1170 |
| AccI | GTMKAC | 1 cut(s) | 1911 |
| AccII | CGCG | 9 cut(s) | 88, 480, 745, 747, 1407, 1632, 1761, 1984, 1986 |
| AclI | AACGTT | 1 cut(s) | 866 |
| AclWI | GGATC | 1 cut(s) | 759 |
| AcoI | YGGCCR | 1 cut(s) | 730 |
| AcsI | RAATTY | 4 cut(s) | 14, 307, 585, 1899 |
| AcuI | CTGAAG | 1 cut(s) | 1580 |
| AcyI | GRCGYC | 3 cut(s) | 704, 966, 2119 |
| AfaI | GTAC | 3 cut(s) | 848, 1349, 2083 |
| AflIII | ACRYGT | 1 cut(s) | 86 |
| AjnI | CCWGG | 2 cut(s) | 48, 883 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1193, 1225 |
| Alw21I | GWGCWC | 3 cut(s) | 279, 1206, 1621 |
| Alw26I | GTCTC | 5 cut(s) | 47, 216, 534, 629, 1107 |
| AlwI | GGATC | 1 cut(s) | 759 |
| Ama87I | CYCGRG | 3 cut(s) | 889, 1466, 2088 |
| AoxI | GGCC | 6 cut(s) | 730, 777, 802, 1572, 1791, 2111 |
| ApeKI | GCWGC | 2 cut(s) | 854, 1271 |
| ApoI | RAATTY | 4 cut(s) | 14, 307, 585, 1899 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1709, 1741 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 560, 1145 |
| AspA2I | CCTAGG | 1 cut(s) | 364 |
| AspLEI | GCGC | 8 cut(s) | 401, 505, 747, 968, 1632, 1802, 1986, 1994 |
| AspS9I | GGNCC | 5 cut(s) | 778, 802, 1160, 1322, 1703 |
| AsuC2I | CCSGG | 5 cut(s) | 620, 1807, 2089, 2090, 2141 |
| AsuHPI | GGTGA | 2 cut(s) | 530, 1006 |
| AvaI | CYCGRG | 3 cut(s) | 889, 1466, 2088 |
| AvaII | GGWCC | 3 cut(s) | 1160, 1322, 1703 |
| AvrII | CCTAGG | 1 cut(s) | 364 |
| BaeGI | GKGCMC | 2 cut(s) | 1180, 1861 |
| BanI | GGYRCC | 3 cut(s) | 965, 979, 1289 |
| BanII | GRGCYC | 3 cut(s) | 1058, 1206, 2146 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1679, 1711 |
| Bbv12I | GWGCWC | 3 cut(s) | 279, 1206, 1621 |
| BbvCI | CCTCAGC | 2 cut(s) | 906, 1847 |
| BbvI | GCAGC | 2 cut(s) | 866, 1283 |
| BccI | CCATC | 8 cut(s) | 136, 651, 908, 956, 1096, 1401, 1501, 1763 |
| BceAI | ACGGC | 3 cut(s) | 1892, 2045, 2064 |
| BciT130I | CCWGG | 2 cut(s) | 50, 885 |
| BciVI | GTATCC | 2 cut(s) | 370, 672 |
| BclI | TGATCA | 1 cut(s) | 1953 |
| BcnI | CCSGG | 5 cut(s) | 620, 1807, 2089, 2090, 2141 |
| BcoDI | GTCTC | 5 cut(s) | 47, 216, 534, 629, 1107 |
| BfaI | CTAG | 4 cut(s) | 98, 365, 498, 1797 |
| BfmI | CTRYAG | 1 cut(s) | 736 |
| BfoI | RGCGCY | 2 cut(s) | 506, 969 |
| BfuI | GTATCC | 2 cut(s) | 370, 672 |
| BisI | GCNGC | 5 cut(s) | 733, 855, 1272, 1408, 1762 |
| BlnI | CCTAGG | 1 cut(s) | 364 |
| BlsI | GCNGC | 5 cut(s) | 734, 856, 1273, 1409, 1763 |
| Bme1390I | CCNGG | 7 cut(s) | 50, 620, 885, 1807, 2089, 2090, 2141 |
| Bme18I | GGWCC | 3 cut(s) | 1160, 1322, 1703 |
| BmeT110I | CYCGRG | 3 cut(s) | 889, 1466, 2088 |
| BmgT120I | GGNCC | 5 cut(s) | 778, 802, 1160, 1322, 1703 |
| BmrFI | CCNGG | 7 cut(s) | 50, 620, 885, 1807, 2089, 2090, 2141 |
| BmrI | ACTGGG | 1 cut(s) | 1325 |
| BmuI | ACTGGG | 1 cut(s) | 1325 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2022 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1045, 1077, 1991, 2023 |
| BpmI | CTGGAG | 2 cut(s) | 71, 2089 |
| Bpu10I | CCTNAGC | 4 cut(s) | 216, 858, 906, 1847 |
| BpuEI | CTTGAG | 4 cut(s) | 653, 1190, 1550, 2054 |
| BpuMI | CCSGG | 5 cut(s) | 620, 1807, 2089, 2090, 2141 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1952 |
| BsaHI | GRCGYC | 3 cut(s) | 704, 966, 2119 |
| BsaI | GGTCTC | 2 cut(s) | 47, 534 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1193, 1223 |
| Bse1I | ACTGG | 2 cut(s) | 543, 1320 |
| Bse3DI | GCAATG | 1 cut(s) | 1149 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1952 |
| BseBI | CCWGG | 2 cut(s) | 50, 885 |
| BseJI | GATNNNNATC | 1 cut(s) | 1952 |
| BseMI | GCAATG | 1 cut(s) | 1149 |
| BseMII | CTCAG | 8 cut(s) | 50, 207, 605, 897, 1022, 1634, 1861, 2159 |
| BseNI | ACTGG | 2 cut(s) | 543, 1320 |
| BseRI | GAGGAG | 5 cut(s) | 128, 923, 1014, 1050, 1613 |
| BseSI | GKGCMC | 2 cut(s) | 1180, 1861 |
| BseXI | GCAGC | 2 cut(s) | 866, 1283 |
| Bsh1236I | CGCG | 9 cut(s) | 88, 480, 745, 747, 1407, 1632, 1761, 1984, 1986 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1168 |
| BshFI | GGCC | 6 cut(s) | 732, 779, 804, 1574, 1793, 2113 |
| BshNI | GGYRCC | 3 cut(s) | 965, 979, 1289 |
| BsiEI | CGRYCG | 1 cut(s) | 1168 |
| BsiHKAI | GWGCWC | 3 cut(s) | 279, 1206, 1621 |
| BsiHKCI | CYCGRG | 3 cut(s) | 889, 1466, 2088 |
| BsiSI | CCGG | 4 cut(s) | 619, 1806, 2089, 2140 |
| BslFI | GGGAC | 2 cut(s) | 699, 1308 |
| BsmAI | GTCTC | 5 cut(s) | 47, 216, 534, 629, 1107 |
| BsmBI | CGTCTC | 1 cut(s) | 1107 |
| BsmFI | GGGAC | 2 cut(s) | 699, 1308 |
| BsmI | GAATGC | 1 cut(s) | 836 |
| BsnI | GGCC | 6 cut(s) | 732, 779, 804, 1574, 1793, 2113 |
| Bso31I | GGTCTC | 2 cut(s) | 47, 534 |
| BsoBI | CYCGRG | 3 cut(s) | 889, 1466, 2088 |
| Bsp1286I | GDGCHC | 7 cut(s) | 279, 1058, 1180, 1206, 1621, 1861, 2146 |
| Bsp143I | GATC | 7 cut(s) | 751, 1492, 1567, 1623, 1947, 1953, 1979 |
| Bsp19I | CCATGG | 1 cut(s) | 58 |
| Bsp68I | TCGCGA | 1 cut(s) | 480 |
| BspANI | GGCC | 6 cut(s) | 732, 779, 804, 1574, 1793, 2113 |
| BspCNI | CTCAG | 8 cut(s) | 49, 208, 604, 898, 1021, 1633, 1860, 2158 |
| BspFNI | CGCG | 9 cut(s) | 88, 480, 745, 747, 1407, 1632, 1761, 1984, 1986 |
| BspHI | TCATGA | 1 cut(s) | 1950 |
| BspPI | GGATC | 1 cut(s) | 759 |
| BspQI | GCTCTTC | 1 cut(s) | 1386 |
| BspT107I | GGYRCC | 3 cut(s) | 965, 979, 1289 |
| BspTNI | GGTCTC | 2 cut(s) | 47, 534 |
| BsrBI | CCGCTC | 3 cut(s) | 476, 722, 1170 |
| BsrDI | GCAATG | 1 cut(s) | 1149 |
| BsrI | ACTGG | 2 cut(s) | 543, 1320 |
| BssMI | GATC | 7 cut(s) | 751, 1492, 1567, 1623, 1947, 1953, 1979 |
| BssNI | GRCGYC | 3 cut(s) | 704, 966, 2119 |
| BssT1I | CCWWGG | 5 cut(s) | 58, 74, 364, 682, 1575 |
| Bst2UI | CCWGG | 2 cut(s) | 50, 885 |
| Bst4CI | ACNGT | 2 cut(s) | 210, 1931 |
| Bst6I | CTCTTC | 5 cut(s) | 297, 939, 1386, 1677, 1682 |
| BstACI | GRCGYC | 3 cut(s) | 704, 966, 2119 |
| BstC8I | GCNNGC | 5 cut(s) | 7, 478, 1096, 2101, 2111 |
| BstDSI | CCRYGG | 3 cut(s) | 58, 1759, 2061 |
| BstFNI | CGCG | 9 cut(s) | 88, 480, 745, 747, 1407, 1632, 1761, 1984, 1986 |
| BstH2I | RGCGCY | 2 cut(s) | 506, 969 |
| BstHHI | GCGC | 8 cut(s) | 401, 505, 747, 968, 1632, 1802, 1986, 1994 |
| BstKTI | GATC | 7 cut(s) | 754, 1495, 1570, 1626, 1950, 1956, 1982 |
| BstMAI | GTCTC | 5 cut(s) | 47, 216, 534, 629, 1107 |
| BstMBI | GATC | 7 cut(s) | 751, 1492, 1567, 1623, 1947, 1953, 1979 |
| BstMCI | CGRYCG | 1 cut(s) | 1168 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 85, 283, 801, 1277, 1298, 1385, 1404, 1799 |
| BstNI | CCWGG | 2 cut(s) | 50, 885 |
| BstNSI | RCATGY | 1 cut(s) | 2044 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2022 |
| BstSCI | CCNGG | 7 cut(s) | 48, 618, 883, 1805, 2087, 2088, 2139 |
| BstSFI | CTRYAG | 1 cut(s) | 736 |
| BstSLI | GKGCMC | 2 cut(s) | 1180, 1861 |
| BstUI | CGCG | 9 cut(s) | 88, 480, 745, 747, 1407, 1632, 1761, 1984, 1986 |
| BstV1I | GCAGC | 2 cut(s) | 866, 1283 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1267 |
| BsuI | GTATCC | 2 cut(s) | 370, 672 |
| BsuRI | GGCC | 6 cut(s) | 732, 779, 804, 1574, 1793, 2113 |
| BtgI | CCRYGG | 3 cut(s) | 58, 1759, 2061 |
| BtgZI | GCGATG | 3 cut(s) | 975, 1287, 1379 |
| BtuMI | TCGCGA | 1 cut(s) | 480 |
| Cac8I | GCNNGC | 5 cut(s) | 7, 478, 1096, 2101, 2111 |
| CciI | TCATGA | 1 cut(s) | 1950 |
| CfoI | GCGC | 8 cut(s) | 401, 505, 747, 968, 1632, 1802, 1986, 1994 |
| Cfr13I | GGNCC | 5 cut(s) | 778, 802, 1160, 1322, 1703 |
| Cfr42I | CCGCGG | 1 cut(s) | 1762 |
| Cfr9I | CCCGGG | 1 cut(s) | 2088 |
| CseI | GACGC | 3 cut(s) | 94, 693, 1424 |
| Csp6I | GTAC | 3 cut(s) | 847, 1348, 2082 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 248, 283 |
| CviQI | GTAC | 3 cut(s) | 847, 1348, 2082 |
| DinI | GGCGCC | 1 cut(s) | 967 |
| DpnI | GATC | 7 cut(s) | 753, 1494, 1569, 1625, 1949, 1955, 1981 |
| DpnII | GATC | 7 cut(s) | 751, 1492, 1567, 1623, 1947, 1953, 1979 |
| EaeI | YGGCCR | 1 cut(s) | 730 |
| Eam1104I | CTCTTC | 5 cut(s) | 297, 939, 1386, 1677, 1682 |
| EarI | CTCTTC | 5 cut(s) | 297, 939, 1386, 1677, 1682 |
| Ecl136II | GAGCTC | 1 cut(s) | 1204 |
| Eco130I | CCWWGG | 5 cut(s) | 58, 74, 364, 682, 1575 |
| Eco147I | AGGCCT | 1 cut(s) | 2113 |
| Eco24I | GRGCYC | 3 cut(s) | 1058, 1206, 2146 |
| Eco31I | GGTCTC | 2 cut(s) | 47, 534 |
| Eco32I | GATATC | 1 cut(s) | 169 |
| Eco47I | GGWCC | 3 cut(s) | 1160, 1322, 1703 |
| Eco53kI | GAGCTC | 1 cut(s) | 1204 |
| Eco57I | CTGAAG | 1 cut(s) | 1580 |
| Eco88I | CYCGRG | 3 cut(s) | 889, 1466, 2088 |
| EcoICRI | GAGCTC | 1 cut(s) | 1204 |
| EcoO109I | RGGNCCY | 3 cut(s) | 1160, 1322, 1703 |
| EcoRI | GAATTC | 1 cut(s) | 307 |
| EcoRII | CCWGG | 2 cut(s) | 48, 883 |
| EcoRV | GATATC | 1 cut(s) | 169 |
| EcoT14I | CCWWGG | 5 cut(s) | 58, 74, 364, 682, 1575 |
| EcoT38I | GRGCYC | 3 cut(s) | 1058, 1206, 2146 |
| EgeI | GGCGCC | 1 cut(s) | 967 |
| EheI | GGCGCC | 1 cut(s) | 967 |
| ErhI | CCWWGG | 5 cut(s) | 58, 74, 364, 682, 1575 |
| Esp3I | CGTCTC | 1 cut(s) | 1107 |
| FaqI | GGGAC | 2 cut(s) | 699, 1308 |
| FauI | CCCGC | 2 cut(s) | 163, 841 |
| FbaI | TGATCA | 1 cut(s) | 1953 |
| FblI | GTMKAC | 1 cut(s) | 1911 |
| Fnu4HI | GCNGC | 5 cut(s) | 733, 855, 1272, 1408, 1762 |
| FriOI | GRGCYC | 3 cut(s) | 1058, 1206, 2146 |
| Fsp4HI | GCNGC | 5 cut(s) | 733, 855, 1272, 1408, 1762 |
| FspBI | CTAG | 4 cut(s) | 98, 365, 498, 1797 |
| FspI | TGCGCA | 1 cut(s) | 1993 |
| GlaI | GCGC | 8 cut(s) | 400, 504, 746, 967, 1631, 1801, 1985, 1993 |
| GluI | GCNGC | 5 cut(s) | 733, 855, 1272, 1408, 1762 |
| GsuI | CTGGAG | 2 cut(s) | 71, 2089 |
| HaeII | RGCGCY | 2 cut(s) | 506, 969 |
| HaeIII | GGCC | 6 cut(s) | 732, 779, 804, 1574, 1793, 2113 |
| HapII | CCGG | 4 cut(s) | 619, 1806, 2089, 2140 |
| HgaI | GACGC | 3 cut(s) | 94, 693, 1424 |
| HhaI | GCGC | 8 cut(s) | 401, 505, 747, 968, 1632, 1802, 1986, 1994 |
| Hin1I | GRCGYC | 3 cut(s) | 704, 966, 2119 |
| Hin6I | GCGC | 8 cut(s) | 399, 503, 745, 966, 1630, 1800, 1984, 1992 |
| HinP1I | GCGC | 8 cut(s) | 399, 503, 745, 966, 1630, 1800, 1984, 1992 |
| HincII | GTYRAC | 1 cut(s) | 1912 |
| HindII | GTYRAC | 1 cut(s) | 1912 |
| HindIII | AAGCTT | 1 cut(s) | 1194 |
| HpaII | CCGG | 4 cut(s) | 619, 1806, 2089, 2140 |
| HphI | GGTGA | 2 cut(s) | 530, 1006 |
| Hpy166II | GTNNAC | 3 cut(s) | 462, 1463, 1912 |
| Hpy8I | GTNNAC | 3 cut(s) | 462, 1463, 1912 |
| HpyAV | CCTTC | 7 cut(s) | 448, 451, 571, 701, 1137, 1303, 1794 |
| HpyCH4III | ACNGT | 2 cut(s) | 210, 1931 |
| HpyCH4IV | ACGT | 4 cut(s) | 226, 866, 1356, 2119 |
| HpyCH4V | TGCA | 5 cut(s) | 164, 286, 922, 1154, 1838 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 85, 283, 801, 1277, 1298, 1385, 1404, 1799 |
| HpySE526I | ACGT | 4 cut(s) | 226, 866, 1356, 2119 |
| Hsp92I | GRCGYC | 3 cut(s) | 704, 966, 2119 |
| HspAI | GCGC | 8 cut(s) | 399, 503, 745, 966, 1630, 1800, 1984, 1992 |
| KasI | GGCGCC | 1 cut(s) | 965 |
| Ksp22I | TGATCA | 1 cut(s) | 1953 |
| KspI | CCGCGG | 1 cut(s) | 1762 |
| Kzo9I | GATC | 7 cut(s) | 751, 1492, 1567, 1623, 1947, 1953, 1979 |
| LguI | GCTCTTC | 1 cut(s) | 1386 |
| LmnI | GCTCC | 3 cut(s) | 1000, 1201, 2108 |
| Lsp1109I | GCAGC | 2 cut(s) | 866, 1283 |
| MaeI | CTAG | 4 cut(s) | 98, 365, 498, 1797 |
| MaeII | ACGT | 4 cut(s) | 226, 866, 1356, 2119 |
| MaeIII | GTNAC | 4 cut(s) | 787, 1579, 2003, 2027 |
| MalI | GATC | 7 cut(s) | 753, 1494, 1569, 1625, 1949, 1955, 1981 |
| MbiI | CCGCTC | 3 cut(s) | 476, 722, 1170 |
| MboI | GATC | 7 cut(s) | 751, 1492, 1567, 1623, 1947, 1953, 1979 |
| MboII | GAAGA | 9 cut(s) | 284, 956, 1138, 1373, 1573, 1694, 1699, 1826, 1932 |
| MhlI | GDGCHC | 7 cut(s) | 279, 1058, 1180, 1206, 1621, 1861, 2146 |
| MluCI | AATT | 8 cut(s) | 14, 307, 430, 585, 671, 1031, 1514, 1899 |
| MluI | ACGCGT | 1 cut(s) | 86 |
| Mly113I | GGCGCC | 1 cut(s) | 966 |
| MlyI | GAGTC | 3 cut(s) | 88, 1342, 2158 |
| MmeI | TCCRAC | 2 cut(s) | 1675, 2034 |
| MroXI | GAANNNNTTC | 2 cut(s) | 560, 1145 |
| MseI | TTAA | 3 cut(s) | 138, 615, 696 |
| MslI | CAYNNNNRTG | 2 cut(s) | 810, 1955 |
| MspA1I | CMGCKG | 1 cut(s) | 1761 |
| MspI | CCGG | 4 cut(s) | 619, 1806, 2089, 2140 |
| MspR9I | CCNGG | 7 cut(s) | 50, 620, 885, 1807, 2089, 2090, 2141 |
| Mva1269I | GAATGC | 1 cut(s) | 836 |
| MvaI | CCWGG | 2 cut(s) | 50, 885 |
| MvnI | CGCG | 9 cut(s) | 88, 480, 745, 747, 1407, 1632, 1761, 1984, 1986 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 85, 283, 801, 1277, 1298, 1385, 1404, 1799 |
| NarI | GGCGCC | 1 cut(s) | 966 |
| NciI | CCSGG | 5 cut(s) | 620, 1807, 2089, 2090, 2141 |
| NcoI | CCATGG | 1 cut(s) | 58 |
| NdeII | GATC | 7 cut(s) | 751, 1492, 1567, 1623, 1947, 1953, 1979 |
| NmuCI | GTSAC | 1 cut(s) | 2003 |
| NruI | TCGCGA | 1 cut(s) | 480 |
| NsbI | TGCGCA | 1 cut(s) | 1993 |
| NspI | RCATGY | 1 cut(s) | 2044 |
| PaeR7I | CTCGAG | 1 cut(s) | 1466 |
| PagI | TCATGA | 1 cut(s) | 1950 |
| PceI | AGGCCT | 1 cut(s) | 2113 |
| PciSI | GCTCTTC | 1 cut(s) | 1386 |
| PctI | GAATGC | 1 cut(s) | 836 |
| PdmI | GAANNNNTTC | 2 cut(s) | 560, 1145 |
| PfeI | GAWTC | 7 cut(s) | 929, 1015, 1247, 1456, 1663, 2048, 2156 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 75 |
| PkrI | GCNGC | 5 cut(s) | 734, 856, 1273, 1409, 1763 |
| PleI | GAGTC | 3 cut(s) | 88, 1341, 2157 |
| PluTI | GGCGCC | 1 cut(s) | 969 |
| PpsI | GAGTC | 3 cut(s) | 88, 1341, 2157 |
| PpuMI | RGGWCCY | 3 cut(s) | 1160, 1322, 1703 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2022 |
| Psp124BI | GAGCTC | 1 cut(s) | 1206 |
| Psp1406I | AACGTT | 1 cut(s) | 866 |
| Psp5II | RGGWCCY | 3 cut(s) | 1160, 1322, 1703 |
| Psp6I | CCWGG | 2 cut(s) | 48, 883 |
| PspGI | CCWGG | 2 cut(s) | 48, 883 |
| PspPI | GGNCC | 5 cut(s) | 778, 802, 1160, 1322, 1703 |
| PspPPI | RGGWCCY | 3 cut(s) | 1160, 1322, 1703 |
| PspXI | VCTCGAGB | 1 cut(s) | 1466 |
| RruI | TCGCGA | 1 cut(s) | 480 |
| RsaI | GTAC | 3 cut(s) | 848, 1349, 2083 |
| RsaNI | GTAC | 3 cut(s) | 847, 1348, 2082 |
| RseI | CAYNNNNRTG | 2 cut(s) | 810, 1955 |
| SacI | GAGCTC | 1 cut(s) | 1206 |
| SacII | CCGCGG | 1 cut(s) | 1762 |
| SalI | GTCGAC | 1 cut(s) | 1910 |
| SapI | GCTCTTC | 1 cut(s) | 1386 |
| SaqAI | TTAA | 3 cut(s) | 138, 615, 696 |
| SatI | GCNGC | 5 cut(s) | 733, 855, 1272, 1408, 1762 |
| Sau3AI | GATC | 7 cut(s) | 751, 1492, 1567, 1623, 1947, 1953, 1979 |
| Sau96I | GGNCC | 5 cut(s) | 778, 802, 1160, 1322, 1703 |
| SchI | GAGTC | 3 cut(s) | 88, 1342, 2158 |
| ScrFI | CCNGG | 7 cut(s) | 50, 620, 885, 1807, 2089, 2090, 2141 |
| SduI | GDGCHC | 7 cut(s) | 279, 1058, 1180, 1206, 1621, 1861, 2146 |
| SfcI | CTRYAG | 1 cut(s) | 736 |
| SfoI | GGCGCC | 1 cut(s) | 967 |
| Sfr274I | CTCGAG | 1 cut(s) | 1466 |
| Sfr303I | CCGCGG | 1 cut(s) | 1762 |
| SgrBI | CCGCGG | 1 cut(s) | 1762 |
| SgrDI | CGTCGACG | 1 cut(s) | 1910 |
| SinI | GGWCC | 3 cut(s) | 1160, 1322, 1703 |
| SlaI | CTCGAG | 1 cut(s) | 1466 |
| SmaI | CCCGGG | 1 cut(s) | 2090 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 810, 1955 |
| SmlI | CTYRAG | 5 cut(s) | 632, 1205, 1466, 1529, 2033 |
| SmoI | CTYRAG | 5 cut(s) | 632, 1205, 1466, 1529, 2033 |
| Sse9I | AATT | 8 cut(s) | 14, 307, 430, 585, 671, 1031, 1514, 1899 |
| SseBI | AGGCCT | 1 cut(s) | 2113 |
| SspDI | GGCGCC | 1 cut(s) | 965 |
| SspI | AATATT | 1 cut(s) | 187 |
| SspMI | CTAG | 4 cut(s) | 98, 365, 498, 1797 |
| SstI | GAGCTC | 1 cut(s) | 1206 |
| StuI | AGGCCT | 1 cut(s) | 2113 |
| StyD4I | CCNGG | 7 cut(s) | 48, 618, 883, 1805, 2087, 2088, 2139 |
| StyI | CCWWGG | 5 cut(s) | 58, 74, 364, 682, 1575 |
| TaaI | ACNGT | 2 cut(s) | 210, 1931 |
| TaiI | ACGT | 4 cut(s) | 229, 869, 1359, 2122 |
| TaqII | GACCGA | 1 cut(s) | 269 |
| TasI | AATT | 8 cut(s) | 14, 307, 430, 585, 671, 1031, 1514, 1899 |
| TauI | GCSGC | 3 cut(s) | 735, 1410, 1764 |
| TfiI | GAWTC | 7 cut(s) | 929, 1015, 1247, 1456, 1663, 2048, 2156 |
| Tru1I | TTAA | 3 cut(s) | 138, 615, 696 |
| Tru9I | TTAA | 3 cut(s) | 138, 615, 696 |
| TseFI | GTSAC | 1 cut(s) | 2003 |
| TseI | GCWGC | 2 cut(s) | 854, 1271 |
| Tsp45I | GTSAC | 1 cut(s) | 2003 |
| TspDTI | ATGAA | 6 cut(s) | 467, 829, 1138, 1695, 1827, 2084 |
| TspGWI | ACGGA | 2 cut(s) | 1149, 1513 |
| TspMI | CCCGGG | 1 cut(s) | 2088 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 75 |
| VpaK11BI | GGWCC | 3 cut(s) | 1160, 1322, 1703 |
| XapI | RAATTY | 4 cut(s) | 14, 307, 585, 1899 |
| XbaI | TCTAGA | 1 cut(s) | 97 |
| XceI | RCATGY | 1 cut(s) | 2044 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1336 |
| XhoI | CTCGAG | 1 cut(s) | 1466 |
| XmaI | CCCGGG | 1 cut(s) | 2088 |
| XmaJI | CCTAGG | 1 cut(s) | 364 |
| XmiI | GTMKAC | 1 cut(s) | 1911 |
| XmnI | GAANNNNTTC | 2 cut(s) | 560, 1145 |
| XspI | CTAG | 4 cut(s) | 98, 365, 498, 1797 |
| ZraI | GACGTC | 1 cut(s) | 2120 |