Belongs to the short-chain dehydrogenases reductases (SDR) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 684 |
| AciI | CCGC | 4 cut(s) | 23, 141, 665, 684 |
| AclWI | GGATC | 1 cut(s) | 99 |
| AcsI | RAATTY | 1 cut(s) | 119 |
| AcuI | CTGAAG | 3 cut(s) | 209, 360, 753 |
| AfaI | GTAC | 1 cut(s) | 404 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 422 |
| AgeI | ACCGGT | 1 cut(s) | 348 |
| AgsI | TTSAA | 4 cut(s) | 136, 221, 303, 468 |
| AjnI | CCWGG | 1 cut(s) | 517 |
| AleI | CACNNNNGTG | 1 cut(s) | 519 |
| AluBI | AGCT | 6 cut(s) | 344, 436, 440, 671, 731, 757 |
| AluI | AGCT | 6 cut(s) | 344, 436, 440, 671, 731, 757 |
| Alw21I | GWGCWC | 1 cut(s) | 600 |
| AlwI | GGATC | 1 cut(s) | 99 |
| AoxI | GGCC | 1 cut(s) | 413 |
| ApeKI | GCWGC | 2 cut(s) | 20, 635 |
| ApoI | RAATTY | 1 cut(s) | 119 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 253, 285 |
| AseI | ATTAAT | 1 cut(s) | 579 |
| AsiGI | ACCGGT | 1 cut(s) | 348 |
| AsuHPI | GGTGA | 5 cut(s) | 338, 506, 523, 691, 698 |
| AsuNHI | GCTAGC | 1 cut(s) | 436 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 394, 427 |
| Bbv12I | GWGCWC | 1 cut(s) | 600 |
| BbvI | GCAGC | 2 cut(s) | 7, 647 |
| BccI | CCATC | 2 cut(s) | 403, 479 |
| BciT130I | CCWGG | 1 cut(s) | 519 |
| BciVI | GTATCC | 2 cut(s) | 271, 343 |
| BfaI | CTAG | 1 cut(s) | 437 |
| BfuI | GTATCC | 2 cut(s) | 271, 343 |
| BisI | GCNGC | 2 cut(s) | 21, 636 |
| BlsI | GCNGC | 2 cut(s) | 22, 637 |
| Bme1390I | CCNGG | 1 cut(s) | 519 |
| BmrFI | CCNGG | 1 cut(s) | 519 |
| BmsI | GCATC | 3 cut(s) | 67, 134, 670 |
| BmtI | GCTAGC | 1 cut(s) | 440 |
| BpuEI | CTTGAG | 1 cut(s) | 711 |
| BsaJI | CCNNGG | 2 cut(s) | 81, 517 |
| BsaWI | WCCGGW | 1 cut(s) | 348 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 422 |
| Bse118I | RCCGGY | 1 cut(s) | 348 |
| BseBI | CCWGG | 1 cut(s) | 519 |
| BseDI | CCNNGG | 2 cut(s) | 81, 517 |
| BseGI | GGATG | 1 cut(s) | 661 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 422 |
| BseXI | GCAGC | 2 cut(s) | 7, 647 |
| BsgI | GTGCAG | 1 cut(s) | 279 |
| BshFI | GGCC | 1 cut(s) | 415 |
| BshTI | ACCGGT | 1 cut(s) | 348 |
| BsiHKAI | GWGCWC | 1 cut(s) | 600 |
| BsiSI | CCGG | 1 cut(s) | 349 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 422 |
| BsnI | GGCC | 1 cut(s) | 415 |
| Bsp1286I | GDGCHC | 1 cut(s) | 600 |
| Bsp143I | GATC | 1 cut(s) | 91 |
| Bsp19I | CCATGG | 1 cut(s) | 81 |
| BspACI | CCGC | 4 cut(s) | 23, 141, 665, 684 |
| BspANI | GGCC | 1 cut(s) | 415 |
| BspOI | GCTAGC | 1 cut(s) | 440 |
| BspPI | GGATC | 1 cut(s) | 99 |
| BsrBI | CCGCTC | 1 cut(s) | 684 |
| BsrFI | RCCGGY | 1 cut(s) | 348 |
| BssAI | RCCGGY | 1 cut(s) | 348 |
| BssECI | CCNNGG | 2 cut(s) | 81, 517 |
| BssMI | GATC | 1 cut(s) | 91 |
| BssT1I | CCWWGG | 1 cut(s) | 81 |
| Bst2UI | CCWGG | 1 cut(s) | 519 |
| Bst4CI | ACNGT | 1 cut(s) | 704 |
| BstC8I | GCNNGC | 4 cut(s) | 417, 438, 453, 669 |
| BstDEI | CTNAG | 1 cut(s) | 95 |
| BstDSI | CCRYGG | 1 cut(s) | 81 |
| BstF5I | GGATG | 1 cut(s) | 661 |
| BstKTI | GATC | 1 cut(s) | 94 |
| BstMBI | GATC | 1 cut(s) | 91 |
| BstNI | CCWGG | 1 cut(s) | 519 |
| BstNSI | RCATGY | 1 cut(s) | 455 |
| BstSCI | CCNGG | 1 cut(s) | 517 |
| BstV1I | GCAGC | 2 cut(s) | 7, 647 |
| BstX2I | RGATCY | 1 cut(s) | 91 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 88 |
| BstYI | RGATCY | 1 cut(s) | 91 |
| BsuI | GTATCC | 2 cut(s) | 271, 343 |
| BsuRI | GGCC | 1 cut(s) | 415 |
| BtgI | CCRYGG | 1 cut(s) | 81 |
| BtsCI | GGATG | 1 cut(s) | 661 |
| BtsI | GCAGTG | 2 cut(s) | 63, 656 |
| BtsIMutI | CAGTG | 2 cut(s) | 63, 656 |
| Cac8I | GCNNGC | 4 cut(s) | 417, 438, 453, 669 |
| Cfr10I | RCCGGY | 1 cut(s) | 348 |
| Csp6I | GTAC | 1 cut(s) | 403 |
| CspAI | ACCGGT | 1 cut(s) | 348 |
| CviAII | CATG | 3 cut(s) | 82, 452, 572 |
| CviQI | GTAC | 1 cut(s) | 403 |
| DdeI | CTNAG | 1 cut(s) | 95 |
| DpnI | GATC | 1 cut(s) | 93 |
| DpnII | GATC | 1 cut(s) | 91 |
| Eco130I | CCWWGG | 1 cut(s) | 81 |
| Eco57I | CTGAAG | 3 cut(s) | 209, 360, 753 |
| EcoRII | CCWGG | 1 cut(s) | 517 |
| EcoT14I | CCWWGG | 1 cut(s) | 81 |
| ErhI | CCWWGG | 1 cut(s) | 81 |
| FaeI | CATG | 3 cut(s) | 85, 455, 575 |
| FatI | CATG | 3 cut(s) | 81, 451, 571 |
| FauI | CCCGC | 1 cut(s) | 16 |
| FauNDI | CATATG | 1 cut(s) | 421 |
| Fnu4HI | GCNGC | 2 cut(s) | 21, 636 |
| FokI | GGATG | 1 cut(s) | 648 |
| Fsp4HI | GCNGC | 2 cut(s) | 21, 636 |
| FspBI | CTAG | 1 cut(s) | 437 |
| GluI | GCNGC | 2 cut(s) | 21, 636 |
| HaeIII | GGCC | 1 cut(s) | 415 |
| HapII | CCGG | 1 cut(s) | 349 |
| Hin1II | CATG | 3 cut(s) | 85, 455, 575 |
| HincII | GTYRAC | 1 cut(s) | 261 |
| HindII | GTYRAC | 1 cut(s) | 261 |
| HinfI | GANTC | 2 cut(s) | 369, 384 |
| HpaII | CCGG | 1 cut(s) | 349 |
| HphI | GGTGA | 5 cut(s) | 338, 506, 523, 691, 698 |
| Hpy166II | GTNNAC | 1 cut(s) | 261 |
| Hpy188I | TCNGA | 3 cut(s) | 130, 340, 700 |
| Hpy188III | TCNNGA | 2 cut(s) | 115, 362 |
| Hpy8I | GTNNAC | 1 cut(s) | 261 |
| HpyAV | CCTTC | 2 cut(s) | 308, 359 |
| HpyCH4III | ACNGT | 1 cut(s) | 704 |
| HpyCH4IV | ACGT | 1 cut(s) | 298 |
| HpyF3I | CTNAG | 1 cut(s) | 95 |
| HpySE526I | ACGT | 1 cut(s) | 298 |
| Hsp92II | CATG | 3 cut(s) | 85, 455, 575 |
| Kzo9I | GATC | 1 cut(s) | 91 |
| LmnI | GCTCC | 1 cut(s) | 632 |
| Lsp1109I | GCAGC | 2 cut(s) | 7, 647 |
| LweI | GCATC | 3 cut(s) | 67, 134, 670 |
| MaeI | CTAG | 1 cut(s) | 437 |
| MaeII | ACGT | 1 cut(s) | 298 |
| MalI | GATC | 1 cut(s) | 93 |
| MbiI | CCGCTC | 1 cut(s) | 684 |
| MboI | GATC | 1 cut(s) | 91 |
| MboII | GAAGA | 2 cut(s) | 494, 746 |
| MflI | RGATCY | 1 cut(s) | 91 |
| MhlI | GDGCHC | 1 cut(s) | 600 |
| MluCI | AATT | 6 cut(s) | 72, 119, 165, 180, 321, 525 |
| MmeI | TCCRAC | 2 cut(s) | 69, 255 |
| MnlI | CCTC | 4 cut(s) | 3, 162, 346, 735 |
| MseI | TTAA | 6 cut(s) | 51, 164, 168, 579, 591, 615 |
| MslI | CAYNNNNRTG | 1 cut(s) | 519 |
| MspA1I | CMGCKG | 1 cut(s) | 143 |
| MspI | CCGG | 1 cut(s) | 349 |
| MspR9I | CCNGG | 1 cut(s) | 519 |
| MvaI | CCWGG | 1 cut(s) | 519 |
| NcoI | CCATGG | 1 cut(s) | 81 |
| NdeI | CATATG | 1 cut(s) | 421 |
| NdeII | GATC | 1 cut(s) | 91 |
| NheI | GCTAGC | 1 cut(s) | 436 |
| NlaIII | CATG | 3 cut(s) | 85, 455, 575 |
| NspI | RCATGY | 1 cut(s) | 455 |
| OliI | CACNNNNGTG | 1 cut(s) | 519 |
| PacI | TTAATTAA | 1 cut(s) | 168 |
| PaeI | GCATGC | 1 cut(s) | 455 |
| PfeI | GAWTC | 2 cut(s) | 369, 384 |
| PinAI | ACCGGT | 1 cut(s) | 348 |
| PkrI | GCNGC | 2 cut(s) | 22, 637 |
| PshBI | ATTAAT | 1 cut(s) | 579 |
| Psp6I | CCWGG | 1 cut(s) | 517 |
| PspGI | CCWGG | 1 cut(s) | 517 |
| PsuI | RGATCY | 1 cut(s) | 91 |
| RsaI | GTAC | 1 cut(s) | 404 |
| RsaNI | GTAC | 1 cut(s) | 403 |
| RseI | CAYNNNNRTG | 1 cut(s) | 519 |
| SaqAI | TTAA | 6 cut(s) | 51, 164, 168, 579, 591, 615 |
| SatI | GCNGC | 2 cut(s) | 21, 636 |
| Sau3AI | GATC | 1 cut(s) | 91 |
| ScrFI | CCNGG | 1 cut(s) | 519 |
| SduI | GDGCHC | 1 cut(s) | 600 |
| SetI | ASST | 9 cut(s) | 250, 301, 346, 438, 442, 673, 727, 733, 759 |
| SfaNI | GCATC | 3 cut(s) | 67, 134, 670 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 519 |
| SmlI | CTYRAG | 1 cut(s) | 726 |
| SmoI | CTYRAG | 1 cut(s) | 726 |
| SphI | GCATGC | 1 cut(s) | 455 |
| Sse9I | AATT | 6 cut(s) | 72, 119, 165, 180, 321, 525 |
| SsiI | CCGC | 4 cut(s) | 23, 141, 665, 684 |
| SspI | AATATT | 2 cut(s) | 692, 709 |
| SspMI | CTAG | 1 cut(s) | 437 |
| StyD4I | CCNGG | 1 cut(s) | 517 |
| StyI | CCWWGG | 1 cut(s) | 81 |
| TaaI | ACNGT | 1 cut(s) | 704 |
| TaiI | ACGT | 1 cut(s) | 301 |
| TasI | AATT | 6 cut(s) | 72, 119, 165, 180, 321, 525 |
| TfiI | GAWTC | 2 cut(s) | 369, 384 |
| Tru1I | TTAA | 6 cut(s) | 51, 164, 168, 579, 591, 615 |
| Tru9I | TTAA | 6 cut(s) | 51, 164, 168, 579, 591, 615 |
| TscAI | CASTG | 2 cut(s) | 70, 663 |
| TseI | GCWGC | 2 cut(s) | 20, 635 |
| TspDTI | ATGAA | 3 cut(s) | 69, 90, 299 |
| TspGWI | ACGGA | 2 cut(s) | 362, 533 |
| TspRI | CASTG | 2 cut(s) | 70, 663 |
| VspI | ATTAAT | 1 cut(s) | 579 |
| XapI | RAATTY | 1 cut(s) | 119 |
| XceI | RCATGY | 1 cut(s) | 455 |
| XspI | CTAG | 1 cut(s) | 437 |