Short-chain dehydrogenase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 405 |
| Acc65I | GGTACC | 1 cut(s) | 445 |
| AccB1I | GGYRCC | 2 cut(s) | 267, 445 |
| AccI | GTMKAC | 1 cut(s) | 5 |
| AccII | CGCG | 1 cut(s) | 380 |
| AciI | CCGC | 3 cut(s) | 225, 342, 673 |
| AcoI | YGGCCR | 3 cut(s) | 634, 670, 718 |
| AfaI | GTAC | 3 cut(s) | 124, 170, 447 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 441, 460, 561, 591 |
| AgsI | TTSAA | 4 cut(s) | 208, 502, 545, 652 |
| AleI | CACNNNNGTG | 1 cut(s) | 521 |
| AluBI | AGCT | 2 cut(s) | 188, 222 |
| AluI | AGCT | 2 cut(s) | 188, 222 |
| Alw21I | GWGCWC | 1 cut(s) | 197 |
| Alw26I | GTCTC | 2 cut(s) | 436, 688 |
| AoxI | GGCC | 4 cut(s) | 634, 670, 718, 733 |
| ApeKI | GCWGC | 3 cut(s) | 185, 222, 382 |
| Asp718I | GGTACC | 1 cut(s) | 445 |
| AspLEI | GCGC | 2 cut(s) | 382, 406 |
| AspS9I | GGNCC | 1 cut(s) | 508 |
| AsuC2I | CCSGG | 1 cut(s) | 276 |
| AsuHPI | GGTGA | 3 cut(s) | 77, 122, 548 |
| AvaII | GGWCC | 1 cut(s) | 508 |
| BanI | GGYRCC | 2 cut(s) | 267, 445 |
| Bbv12I | GWGCWC | 1 cut(s) | 197 |
| BbvCI | CCTCAGC | 1 cut(s) | 360 |
| BbvI | GCAGC | 3 cut(s) | 197, 209, 394 |
| BccI | CCATC | 3 cut(s) | 62, 563, 662 |
| BceAI | ACGGC | 3 cut(s) | 187, 355, 649 |
| BcnI | CCSGG | 1 cut(s) | 276 |
| BcoDI | GTCTC | 2 cut(s) | 436, 688 |
| BfaI | CTAG | 1 cut(s) | 591 |
| BfmI | CTRYAG | 1 cut(s) | 678 |
| BisI | GCNGC | 4 cut(s) | 186, 223, 383, 673 |
| BlsI | GCNGC | 4 cut(s) | 187, 224, 384, 674 |
| Bme1390I | CCNGG | 1 cut(s) | 276 |
| Bme18I | GGWCC | 1 cut(s) | 508 |
| BmgT120I | GGNCC | 1 cut(s) | 508 |
| BmiI | GGNNCC | 2 cut(s) | 269, 447 |
| BmrFI | CCNGG | 1 cut(s) | 276 |
| BmrI | ACTGGG | 1 cut(s) | 607 |
| BmsI | GCATC | 3 cut(s) | 73, 103, 373 |
| BmuI | ACTGGG | 1 cut(s) | 607 |
| Bpu10I | CCTNAGC | 1 cut(s) | 360 |
| BpuEI | CTTGAG | 1 cut(s) | 238 |
| BpuMI | CCSGG | 1 cut(s) | 276 |
| BsaJI | CCNNGG | 1 cut(s) | 163 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 63, 93 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 441, 460, 561, 591 |
| Bse1I | ACTGG | 3 cut(s) | 577, 602, 663 |
| BseDI | CCNNGG | 1 cut(s) | 163 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 441, 460, 561, 591 |
| BseMII | CTCAG | 2 cut(s) | 182, 374 |
| BseNI | ACTGG | 3 cut(s) | 577, 602, 663 |
| BseRI | GAGGAG | 1 cut(s) | 348 |
| BseXI | GCAGC | 3 cut(s) | 197, 209, 394 |
| Bsh1236I | CGCG | 1 cut(s) | 380 |
| BshFI | GGCC | 4 cut(s) | 636, 672, 720, 735 |
| BshNI | GGYRCC | 2 cut(s) | 267, 445 |
| BsiHKAI | GWGCWC | 1 cut(s) | 197 |
| BsiSI | CCGG | 3 cut(s) | 276, 701, 732 |
| BslFI | GGGAC | 1 cut(s) | 410 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 441, 460, 561, 591 |
| BsmAI | GTCTC | 2 cut(s) | 436, 688 |
| BsmFI | GGGAC | 1 cut(s) | 410 |
| BsnI | GGCC | 4 cut(s) | 636, 672, 720, 735 |
| Bsp1286I | GDGCHC | 1 cut(s) | 197 |
| Bsp143I | GATC | 2 cut(s) | 240, 373 |
| BspACI | CCGC | 3 cut(s) | 225, 342, 673 |
| BspANI | GGCC | 4 cut(s) | 636, 672, 720, 735 |
| BspCNI | CTCAG | 2 cut(s) | 183, 373 |
| BspFNI | CGCG | 1 cut(s) | 380 |
| BspLI | GGNNCC | 2 cut(s) | 269, 447 |
| BspT107I | GGYRCC | 2 cut(s) | 267, 445 |
| BsrI | ACTGG | 3 cut(s) | 577, 602, 663 |
| BssECI | CCNNGG | 1 cut(s) | 163 |
| BssMI | GATC | 2 cut(s) | 240, 373 |
| BssT1I | CCWWGG | 1 cut(s) | 163 |
| Bst4CI | ACNGT | 3 cut(s) | 10, 488, 679 |
| BstC8I | GCNNGC | 2 cut(s) | 232, 706 |
| BstDEI | CTNAG | 2 cut(s) | 191, 360 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 439 |
| BstFNI | CGCG | 1 cut(s) | 380 |
| BstHHI | GCGC | 2 cut(s) | 382, 406 |
| BstKTI | GATC | 2 cut(s) | 243, 376 |
| BstMAI | GTCTC | 2 cut(s) | 436, 688 |
| BstMBI | GATC | 2 cut(s) | 240, 373 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 222, 231, 348, 403 |
| BstSCI | CCNGG | 1 cut(s) | 274 |
| BstSFI | CTRYAG | 1 cut(s) | 678 |
| BstUI | CGCG | 1 cut(s) | 380 |
| BstV1I | GCAGC | 3 cut(s) | 197, 209, 394 |
| BsuRI | GGCC | 4 cut(s) | 636, 672, 720, 735 |
| BtsI | GCAGTG | 1 cut(s) | 291 |
| BtsIMutI | CAGTG | 3 cut(s) | 291, 670, 675 |
| Cac8I | GCNNGC | 2 cut(s) | 232, 706 |
| CfoI | GCGC | 2 cut(s) | 382, 406 |
| Cfr13I | GGNCC | 1 cut(s) | 508 |
| Csp6I | GTAC | 3 cut(s) | 123, 169, 446 |
| CviAII | CATG | 3 cut(s) | 56, 89, 586 |
| CviQI | GTAC | 3 cut(s) | 123, 169, 446 |
| DdeI | CTNAG | 2 cut(s) | 191, 360 |
| DpnI | GATC | 2 cut(s) | 242, 375 |
| DpnII | GATC | 2 cut(s) | 240, 373 |
| EaeI | YGGCCR | 3 cut(s) | 634, 670, 718 |
| Eco130I | CCWWGG | 1 cut(s) | 163 |
| Eco47I | GGWCC | 1 cut(s) | 508 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 439 |
| EcoT14I | CCWWGG | 1 cut(s) | 163 |
| ErhI | CCWWGG | 1 cut(s) | 163 |
| FaeI | CATG | 3 cut(s) | 59, 92, 589 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 433, 465 |
| FaqI | GGGAC | 1 cut(s) | 410 |
| FatI | CATG | 3 cut(s) | 55, 88, 585 |
| FauI | CCCGC | 1 cut(s) | 218 |
| FblI | GTMKAC | 1 cut(s) | 5 |
| Fnu4HI | GCNGC | 4 cut(s) | 186, 223, 383, 673 |
| Fsp4HI | GCNGC | 4 cut(s) | 186, 223, 383, 673 |
| FspBI | CTAG | 1 cut(s) | 591 |
| FspI | TGCGCA | 1 cut(s) | 405 |
| GlaI | GCGC | 2 cut(s) | 381, 405 |
| GluI | GCNGC | 4 cut(s) | 186, 223, 383, 673 |
| HaeIII | GGCC | 4 cut(s) | 636, 672, 720, 735 |
| HapII | CCGG | 3 cut(s) | 276, 701, 732 |
| HhaI | GCGC | 2 cut(s) | 382, 406 |
| Hin1II | CATG | 3 cut(s) | 59, 92, 589 |
| Hin6I | GCGC | 2 cut(s) | 380, 404 |
| HinP1I | GCGC | 2 cut(s) | 380, 404 |
| HincII | GTYRAC | 1 cut(s) | 6 |
| HindII | GTYRAC | 1 cut(s) | 6 |
| HinfI | GANTC | 2 cut(s) | 420, 474 |
| HpaII | CCGG | 3 cut(s) | 276, 701, 732 |
| HphI | GGTGA | 3 cut(s) | 77, 122, 548 |
| Hpy166II | GTNNAC | 1 cut(s) | 6 |
| Hpy188I | TCNGA | 4 cut(s) | 39, 192, 473, 606 |
| Hpy188III | TCNNGA | 1 cut(s) | 567 |
| Hpy8I | GTNNAC | 1 cut(s) | 6 |
| Hpy99I | CGWCG | 2 cut(s) | 10, 635 |
| HpyAV | CCTTC | 3 cut(s) | 281, 435, 454 |
| HpyCH4III | ACNGT | 3 cut(s) | 10, 488, 679 |
| HpyCH4IV | ACGT | 1 cut(s) | 522 |
| HpyCH4V | TGCA | 1 cut(s) | 351 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 222, 231, 348, 403 |
| HpyF3I | CTNAG | 2 cut(s) | 191, 360 |
| HpySE526I | ACGT | 1 cut(s) | 522 |
| Hsp92II | CATG | 3 cut(s) | 59, 92, 589 |
| HspAI | GCGC | 2 cut(s) | 380, 404 |
| KpnI | GGTACC | 1 cut(s) | 449 |
| Kzo9I | GATC | 2 cut(s) | 240, 373 |
| Lsp1109I | GCAGC | 3 cut(s) | 197, 209, 394 |
| LweI | GCATC | 3 cut(s) | 73, 103, 373 |
| MaeI | CTAG | 1 cut(s) | 591 |
| MaeII | ACGT | 1 cut(s) | 522 |
| MaeIII | GTNAC | 3 cut(s) | 289, 488, 655 |
| MalI | GATC | 2 cut(s) | 242, 375 |
| MboI | GATC | 2 cut(s) | 240, 373 |
| MhlI | GDGCHC | 1 cut(s) | 197 |
| MluCI | AATT | 3 cut(s) | 301, 607, 740 |
| MmeI | TCCRAC | 1 cut(s) | 648 |
| MnlI | CCTC | 8 cut(s) | 65, 127, 321, 366, 369, 638, 653, 746 |
| MseI | TTAA | 1 cut(s) | 748 |
| MslI | CAYNNNNRTG | 2 cut(s) | 60, 521 |
| MspI | CCGG | 3 cut(s) | 276, 701, 732 |
| MspR9I | CCNGG | 1 cut(s) | 276 |
| MvnI | CGCG | 1 cut(s) | 380 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 222, 231, 348, 403 |
| NciI | CCSGG | 1 cut(s) | 276 |
| NdeII | GATC | 2 cut(s) | 240, 373 |
| NlaIII | CATG | 3 cut(s) | 59, 92, 589 |
| NlaIV | GGNNCC | 2 cut(s) | 269, 447 |
| NmuCI | GTSAC | 3 cut(s) | 289, 488, 655 |
| NsbI | TGCGCA | 1 cut(s) | 405 |
| OliI | CACNNNNGTG | 1 cut(s) | 521 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 384 |
| PfeI | GAWTC | 2 cut(s) | 420, 474 |
| PkrI | GCNGC | 4 cut(s) | 187, 224, 384, 674 |
| PspN4I | GGNNCC | 2 cut(s) | 269, 447 |
| PspPI | GGNCC | 1 cut(s) | 508 |
| RsaI | GTAC | 3 cut(s) | 124, 170, 447 |
| RsaNI | GTAC | 3 cut(s) | 123, 169, 446 |
| RseI | CAYNNNNRTG | 2 cut(s) | 60, 521 |
| SalI | GTCGAC | 1 cut(s) | 4 |
| SaqAI | TTAA | 1 cut(s) | 748 |
| SatI | GCNGC | 4 cut(s) | 186, 223, 383, 673 |
| Sau3AI | GATC | 2 cut(s) | 240, 373 |
| Sau96I | GGNCC | 1 cut(s) | 508 |
| ScrFI | CCNGG | 1 cut(s) | 276 |
| SduI | GDGCHC | 1 cut(s) | 197 |
| SetI | ASST | 8 cut(s) | 76, 156, 190, 224, 451, 525, 630, 657 |
| SfaNI | GCATC | 3 cut(s) | 73, 103, 373 |
| SfcI | CTRYAG | 1 cut(s) | 678 |
| SinI | GGWCC | 1 cut(s) | 508 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 60, 521 |
| SmlI | CTYRAG | 1 cut(s) | 217 |
| SmoI | CTYRAG | 1 cut(s) | 217 |
| Sse9I | AATT | 3 cut(s) | 301, 607, 740 |
| SsiI | CCGC | 3 cut(s) | 225, 342, 673 |
| SspMI | CTAG | 1 cut(s) | 591 |
| StyD4I | CCNGG | 1 cut(s) | 274 |
| StyI | CCWWGG | 1 cut(s) | 163 |
| TaaI | ACNGT | 3 cut(s) | 10, 488, 679 |
| TaiI | ACGT | 1 cut(s) | 525 |
| TaqI | TCGA | 3 cut(s) | 5, 372, 630 |
| TasI | AATT | 3 cut(s) | 301, 607, 740 |
| TauI | GCSGC | 1 cut(s) | 675 |
| TfiI | GAWTC | 2 cut(s) | 420, 474 |
| Tru1I | TTAA | 1 cut(s) | 748 |
| Tru9I | TTAA | 1 cut(s) | 748 |
| TscAI | CASTG | 3 cut(s) | 298, 670, 682 |
| TseFI | GTSAC | 3 cut(s) | 289, 488, 655 |
| TseI | GCWGC | 3 cut(s) | 185, 222, 382 |
| Tsp45I | GTSAC | 3 cut(s) | 289, 488, 655 |
| TspRI | CASTG | 3 cut(s) | 298, 670, 682 |
| VpaK11BI | GGWCC | 1 cut(s) | 508 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 439 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 461 |
| XmiI | GTMKAC | 1 cut(s) | 5 |
| XspI | CTAG | 1 cut(s) | 591 |