Short-chain dehydrogenase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 12, 422, 842 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 987 |
| AccII | CGCG | 3 cut(s) | 205, 207, 532 |
| AciI | CCGC | 1 cut(s) | 912 |
| AclWI | GGATC | 1 cut(s) | 763 |
| AcoI | YGGCCR | 1 cut(s) | 89 |
| AcsI | RAATTY | 1 cut(s) | 603 |
| AcuI | CTGAAG | 1 cut(s) | 731 |
| AcyI | GRCGYC | 1 cut(s) | 706 |
| AfaI | GTAC | 1 cut(s) | 593 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 987 |
| AgsI | TTSAA | 2 cut(s) | 433, 854 |
| AluBI | AGCT | 8 cut(s) | 81, 336, 436, 457, 636, 670, 697, 999 |
| AluI | AGCT | 8 cut(s) | 81, 336, 436, 457, 636, 670, 697, 999 |
| Alw26I | GTCTC | 3 cut(s) | 158, 191, 506 |
| AlwI | GGATC | 1 cut(s) | 763 |
| Ama87I | CYCGRG | 1 cut(s) | 132 |
| AoxI | GGCC | 2 cut(s) | 89, 382 |
| ApeKI | GCWGC | 2 cut(s) | 457, 863 |
| ApoI | RAATTY | 1 cut(s) | 603 |
| AseI | ATTAAT | 1 cut(s) | 621 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 603 |
| AspLEI | GCGC | 2 cut(s) | 207, 780 |
| AspS9I | GGNCC | 1 cut(s) | 382 |
| AsuC2I | CCSGG | 1 cut(s) | 753 |
| AvaI | CYCGRG | 1 cut(s) | 132 |
| BaeGI | GKGCMC | 1 cut(s) | 486 |
| BalI | TGGCCA | 1 cut(s) | 91 |
| BbvI | GCAGC | 2 cut(s) | 444, 875 |
| BccI | CCATC | 4 cut(s) | 100, 137, 588, 897 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 905, 939 |
| BcnI | CCSGG | 1 cut(s) | 753 |
| BcoDI | GTCTC | 3 cut(s) | 158, 191, 506 |
| BfaI | CTAG | 3 cut(s) | 51, 78, 306 |
| BglII | AGATCT | 1 cut(s) | 328 |
| BisI | GCNGC | 3 cut(s) | 458, 864, 913 |
| BlsI | GCNGC | 3 cut(s) | 459, 865, 914 |
| Bme1390I | CCNGG | 1 cut(s) | 753 |
| BmeT110I | CYCGRG | 1 cut(s) | 132 |
| BmgT120I | GGNCC | 1 cut(s) | 382 |
| BmiI | GGNNCC | 1 cut(s) | 175 |
| BmrFI | CCNGG | 1 cut(s) | 753 |
| BmsI | GCATC | 2 cut(s) | 353, 955 |
| BpuEI | CTTGAG | 3 cut(s) | 426, 1001, 1021 |
| BpuMI | CCSGG | 1 cut(s) | 753 |
| BsaHI | GRCGYC | 1 cut(s) | 706 |
| BsaI | GGTCTC | 2 cut(s) | 158, 506 |
| BsaJI | CCNNGG | 1 cut(s) | 378 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 987 |
| Bse1I | ACTGG | 1 cut(s) | 154 |
| Bse3DI | GCAATG | 2 cut(s) | 215, 936 |
| BseDI | CCNNGG | 1 cut(s) | 378 |
| BseGI | GGATG | 4 cut(s) | 28, 396, 748, 889 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 987 |
| BseMI | GCAATG | 2 cut(s) | 215, 936 |
| BseMII | CTCAG | 2 cut(s) | 117, 346 |
| BseNI | ACTGG | 1 cut(s) | 154 |
| BseSI | GKGCMC | 1 cut(s) | 486 |
| BseXI | GCAGC | 2 cut(s) | 444, 875 |
| BseYI | CCCAGC | 1 cut(s) | 817 |
| BsgI | GTGCAG | 1 cut(s) | 882 |
| Bsh1236I | CGCG | 3 cut(s) | 205, 207, 532 |
| BshFI | GGCC | 2 cut(s) | 91, 384 |
| BsiHKCI | CYCGRG | 1 cut(s) | 132 |
| BsiSI | CCGG | 1 cut(s) | 752 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 987 |
| BsmAI | GTCTC | 3 cut(s) | 158, 191, 506 |
| BsnI | GGCC | 2 cut(s) | 91, 384 |
| Bso31I | GGTCTC | 2 cut(s) | 158, 506 |
| BsoBI | CYCGRG | 1 cut(s) | 132 |
| Bsp1286I | GDGCHC | 1 cut(s) | 486 |
| Bsp143I | GATC | 3 cut(s) | 328, 729, 755 |
| Bsp68I | TCGCGA | 1 cut(s) | 532 |
| BspACI | CCGC | 1 cut(s) | 912 |
| BspANI | GGCC | 2 cut(s) | 91, 384 |
| BspCNI | CTCAG | 2 cut(s) | 116, 345 |
| BspFNI | CGCG | 3 cut(s) | 205, 207, 532 |
| BspLI | GGNNCC | 1 cut(s) | 175 |
| BspPI | GGATC | 1 cut(s) | 763 |
| BspTNI | GGTCTC | 2 cut(s) | 158, 506 |
| BsrDI | GCAATG | 2 cut(s) | 215, 936 |
| BsrI | ACTGG | 1 cut(s) | 154 |
| BssECI | CCNNGG | 1 cut(s) | 378 |
| BssMI | GATC | 3 cut(s) | 328, 729, 755 |
| BssNI | GRCGYC | 1 cut(s) | 706 |
| BssT1I | CCWWGG | 1 cut(s) | 378 |
| Bst4CI | ACNGT | 2 cut(s) | 596, 850 |
| Bst6I | CTCTTC | 4 cut(s) | 30, 115, 360, 750 |
| BstACI | GRCGYC | 1 cut(s) | 706 |
| BstC8I | GCNNGC | 1 cut(s) | 672 |
| BstDEI | CTNAG | 3 cut(s) | 103, 332, 478 |
| BstF5I | GGATG | 4 cut(s) | 28, 396, 748, 889 |
| BstFNI | CGCG | 3 cut(s) | 205, 207, 532 |
| BstHHI | GCGC | 2 cut(s) | 207, 780 |
| BstKTI | GATC | 3 cut(s) | 331, 732, 758 |
| BstMAI | GTCTC | 3 cut(s) | 158, 191, 506 |
| BstMBI | GATC | 3 cut(s) | 328, 729, 755 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 703, 965, 974 |
| BstNSI | RCATGY | 3 cut(s) | 689, 796, 876 |
| BstSCI | CCNGG | 1 cut(s) | 751 |
| BstSLI | GKGCMC | 1 cut(s) | 486 |
| BstUI | CGCG | 3 cut(s) | 205, 207, 532 |
| BstV1I | GCAGC | 2 cut(s) | 444, 875 |
| BstX2I | RGATCY | 1 cut(s) | 328 |
| BstYI | RGATCY | 1 cut(s) | 328 |
| BsuRI | GGCC | 2 cut(s) | 91, 384 |
| BtgZI | GCGATG | 2 cut(s) | 186, 513 |
| BtsCI | GGATG | 4 cut(s) | 28, 396, 748, 889 |
| BtsIMutI | CAGTG | 1 cut(s) | 389 |
| BtuMI | TCGCGA | 1 cut(s) | 532 |
| Cac8I | GCNNGC | 1 cut(s) | 672 |
| CfoI | GCGC | 2 cut(s) | 207, 780 |
| Cfr13I | GGNCC | 1 cut(s) | 382 |
| CseI | GACGC | 1 cut(s) | 695 |
| Csp6I | GTAC | 1 cut(s) | 592 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 859, 894 |
| CviAII | CATG | 6 cut(s) | 230, 517, 686, 793, 873, 1006 |
| CviQI | GTAC | 1 cut(s) | 592 |
| DdeI | CTNAG | 3 cut(s) | 103, 332, 478 |
| DpnI | GATC | 3 cut(s) | 330, 731, 757 |
| DpnII | GATC | 3 cut(s) | 328, 729, 755 |
| EaeI | YGGCCR | 1 cut(s) | 89 |
| Eam1104I | CTCTTC | 4 cut(s) | 30, 115, 360, 750 |
| EarI | CTCTTC | 4 cut(s) | 30, 115, 360, 750 |
| Eco130I | CCWWGG | 1 cut(s) | 378 |
| Eco31I | GGTCTC | 2 cut(s) | 158, 506 |
| Eco57I | CTGAAG | 1 cut(s) | 731 |
| Eco88I | CYCGRG | 1 cut(s) | 132 |
| EcoRI | GAATTC | 1 cut(s) | 603 |
| EcoT14I | CCWWGG | 1 cut(s) | 378 |
| ErhI | CCWWGG | 1 cut(s) | 378 |
| FaeI | CATG | 6 cut(s) | 233, 520, 689, 796, 876, 1009 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 270, 302 |
| FatI | CATG | 6 cut(s) | 229, 516, 685, 792, 872, 1005 |
| Fnu4HI | GCNGC | 3 cut(s) | 458, 864, 913 |
| FokI | GGATG | 4 cut(s) | 15, 383, 735, 876 |
| Fsp4HI | GCNGC | 3 cut(s) | 458, 864, 913 |
| FspBI | CTAG | 3 cut(s) | 51, 78, 306 |
| GlaI | GCGC | 2 cut(s) | 206, 779 |
| GluI | GCNGC | 3 cut(s) | 458, 864, 913 |
| GsaI | CCCAGC | 1 cut(s) | 821 |
| HaeIII | GGCC | 2 cut(s) | 91, 384 |
| HapII | CCGG | 1 cut(s) | 752 |
| HgaI | GACGC | 1 cut(s) | 695 |
| HhaI | GCGC | 2 cut(s) | 207, 780 |
| Hin1I | GRCGYC | 1 cut(s) | 706 |
| Hin1II | CATG | 6 cut(s) | 233, 520, 689, 796, 876, 1009 |
| Hin6I | GCGC | 2 cut(s) | 205, 778 |
| HinP1I | GCGC | 2 cut(s) | 205, 778 |
| HindIII | AAGCTT | 2 cut(s) | 434, 668 |
| HinfI | GANTC | 2 cut(s) | 43, 629 |
| HpaII | CCGG | 1 cut(s) | 752 |
| Hpy166II | GTNNAC | 1 cut(s) | 592 |
| Hpy188I | TCNGA | 1 cut(s) | 711 |
| Hpy188III | TCNNGA | 3 cut(s) | 443, 531, 763 |
| Hpy8I | GTNNAC | 1 cut(s) | 592 |
| HpyAV | CCTTC | 4 cut(s) | 593, 706, 713, 805 |
| HpyCH4III | ACNGT | 2 cut(s) | 596, 850 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 703, 965, 974 |
| HpyF3I | CTNAG | 3 cut(s) | 103, 332, 478 |
| Hsp92I | GRCGYC | 1 cut(s) | 706 |
| Hsp92II | CATG | 6 cut(s) | 233, 520, 689, 796, 876, 1009 |
| HspAI | GCGC | 2 cut(s) | 205, 778 |
| Kzo9I | GATC | 3 cut(s) | 328, 729, 755 |
| LmnI | GCTCC | 1 cut(s) | 173 |
| LpnPI | CCDG | 9 cut(s) | 81, 135, 156, 246, 399, 765, 776, 803, 842 |
| Lsp1109I | GCAGC | 2 cut(s) | 444, 875 |
| LweI | GCATC | 2 cut(s) | 353, 955 |
| MaeI | CTAG | 3 cut(s) | 51, 78, 306 |
| MaeIII | GTNAC | 2 cut(s) | 127, 166 |
| MalI | GATC | 3 cut(s) | 330, 731, 757 |
| MboI | GATC | 3 cut(s) | 328, 729, 755 |
| MboII | GAAGA | 6 cut(s) | 17, 102, 377, 482, 564, 737 |
| MflI | RGATCY | 1 cut(s) | 328 |
| MhlI | GDGCHC | 1 cut(s) | 486 |
| MlsI | TGGCCA | 1 cut(s) | 91 |
| MluCI | AATT | 5 cut(s) | 373, 555, 603, 618, 739 |
| MluNI | TGGCCA | 1 cut(s) | 91 |
| MlyI | GAGTC | 1 cut(s) | 52 |
| MmeI | TCCRAC | 1 cut(s) | 489 |
| MnlI | CCTC | 7 cut(s) | 27, 42, 66, 115, 118, 128, 541 |
| Mox20I | TGGCCA | 1 cut(s) | 91 |
| MroXI | GAANNNNTTC | 1 cut(s) | 603 |
| MscI | TGGCCA | 1 cut(s) | 91 |
| MseI | TTAA | 5 cut(s) | 288, 405, 621, 738, 900 |
| MslI | CAYNNNNRTG | 2 cut(s) | 585, 690 |
| Msp20I | TGGCCA | 1 cut(s) | 91 |
| MspI | CCGG | 1 cut(s) | 752 |
| MspR9I | CCNGG | 1 cut(s) | 753 |
| MvnI | CGCG | 3 cut(s) | 205, 207, 532 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 703, 965, 974 |
| NciI | CCSGG | 1 cut(s) | 753 |
| NdeII | GATC | 3 cut(s) | 328, 729, 755 |
| NlaIII | CATG | 6 cut(s) | 233, 520, 689, 796, 876, 1009 |
| NlaIV | GGNNCC | 1 cut(s) | 175 |
| NruI | TCGCGA | 1 cut(s) | 532 |
| NspI | RCATGY | 3 cut(s) | 689, 796, 876 |
| PaeR7I | CTCGAG | 1 cut(s) | 132 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 529 |
| PdmI | GAANNNNTTC | 1 cut(s) | 603 |
| PfeI | GAWTC | 1 cut(s) | 629 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 987 |
| PfoI | TCCNGGA | 1 cut(s) | 751 |
| PkrI | GCNGC | 3 cut(s) | 459, 865, 914 |
| PleI | GAGTC | 1 cut(s) | 51 |
| PpsI | GAGTC | 1 cut(s) | 51 |
| PshBI | ATTAAT | 1 cut(s) | 621 |
| PsiI | TTATAA | 3 cut(s) | 12, 422, 842 |
| PspFI | CCCAGC | 1 cut(s) | 817 |
| PspN4I | GGNNCC | 1 cut(s) | 175 |
| PspPI | GGNCC | 1 cut(s) | 382 |
| PspXI | VCTCGAGB | 1 cut(s) | 132 |
| PsuI | RGATCY | 1 cut(s) | 328 |
| RruI | TCGCGA | 1 cut(s) | 532 |
| RsaI | GTAC | 1 cut(s) | 593 |
| RsaNI | GTAC | 1 cut(s) | 592 |
| RseI | CAYNNNNRTG | 2 cut(s) | 585, 690 |
| SaqAI | TTAA | 5 cut(s) | 288, 405, 621, 738, 900 |
| SatI | GCNGC | 3 cut(s) | 458, 864, 913 |
| Sau3AI | GATC | 3 cut(s) | 328, 729, 755 |
| Sau96I | GGNCC | 1 cut(s) | 382 |
| SchI | GAGTC | 1 cut(s) | 52 |
| ScrFI | CCNGG | 1 cut(s) | 753 |
| SduI | GDGCHC | 1 cut(s) | 486 |
| SetI | ASST | 9 cut(s) | 83, 338, 438, 459, 638, 672, 699, 816, 1001 |
| SfaNI | GCATC | 2 cut(s) | 353, 955 |
| Sfr274I | CTCGAG | 1 cut(s) | 132 |
| SlaI | CTCGAG | 1 cut(s) | 132 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 585, 690 |
| SmlI | CTYRAG | 4 cut(s) | 132, 441, 1000, 1016 |
| SmoI | CTYRAG | 4 cut(s) | 132, 441, 1000, 1016 |
| Sse9I | AATT | 5 cut(s) | 373, 555, 603, 618, 739 |
| SsiI | CCGC | 1 cut(s) | 912 |
| SspI | AATATT | 2 cut(s) | 647, 920 |
| SspMI | CTAG | 3 cut(s) | 51, 78, 306 |
| StyD4I | CCNGG | 1 cut(s) | 751 |
| StyI | CCWWGG | 1 cut(s) | 378 |
| TaaI | ACNGT | 2 cut(s) | 596, 850 |
| TaqI | TCGA | 1 cut(s) | 133 |
| TasI | AATT | 5 cut(s) | 373, 555, 603, 618, 739 |
| TauI | GCSGC | 1 cut(s) | 915 |
| TfiI | GAWTC | 1 cut(s) | 629 |
| Tru1I | TTAA | 5 cut(s) | 288, 405, 621, 738, 900 |
| Tru9I | TTAA | 5 cut(s) | 288, 405, 621, 738, 900 |
| TscAI | CASTG | 1 cut(s) | 396 |
| TseI | GCWGC | 2 cut(s) | 457, 863 |
| TspDTI | ATGAA | 5 cut(s) | 17, 17, 533, 642, 737 |
| TspGWI | ACGGA | 1 cut(s) | 825 |
| TspRI | CASTG | 1 cut(s) | 396 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 987 |
| VspI | ATTAAT | 1 cut(s) | 621 |
| XapI | RAATTY | 1 cut(s) | 603 |
| XceI | RCATGY | 3 cut(s) | 689, 796, 876 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 877 |
| XhoI | CTCGAG | 1 cut(s) | 132 |
| XmnI | GAANNNNTTC | 1 cut(s) | 603 |
| XspI | CTAG | 3 cut(s) | 51, 78, 306 |