Rw2G028400

Short-chain dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000238
Physical Location & Seq
Forward (+)
45410616 .. 45412637
2022 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G028400.1

Sequence Viewer

Length: 930 bp
ATGGGGATATTCAGCAAGAAAGGAGCATCAGGGTTCTCGGCCTCTTCCACAGCAGAACAAGTTACTCAAGGGATTGATGGAATTGGTCTTACTTCAATTGTTACAGGAGCCTCAAGTGGTATTGGCGTAGAGACAGCGCGTGTTCTTGCATTGCGCAGTGTTCATGTAATAATGGCAGTAAGGAATATAGATGCTGGTACCAATGTTAAAGAAGCAATCCTTAAAGAAATCCCCAATGCTAAGATTGATGTCATGGAGTTAGATCTCAGCTCATTGGCATCCGTAAGAAAATTTGCAGCAGAGTATAATTCCAAAGGCCTTCCCTTGAATATCCTCATTAACAATGCAGGGGTCATGGCAGATAAATTCAAGCTATCTCGAGACAACATAGAACTCGATTTTGCAACTAACCATTTAGGTCATTTTCTTCTCACAAATCTTCTGTTAGATAACATGAAACACACATCAAGAAAAAGCAGCATAGAGGGGAGAATTGTTAATGTATCATCAATTGGCCATAGATATATATCCCGTGAAGGAATTCGTTTTGATAGTATTAATGACGAATCAAGATACAACAAATCTGTTGCTTATGCAGAATCCAAGCTCGCCAACATATTGCATGCTAATGAGCTTACAAGGCGCCTCAAGGAAGAAAGGGTTGAGATAACTGCTAATTCTCTTCATCCTGGAATAATTCGCACCAATATTATGCATAATGATATCCGTGTAAAGATACTTTTTTTGAGCAAAACTATTCAGCAGGGAGCAGCAACCACATGCTTTTTGGCACTGAATCCACAGGTTAAGGGAGTAAGCGGTGAATACTATGCGGATTGTAACATTGCCAAACCGACATCTCAGGCAAATGATGCCGATTTGGCTAAGAGACTCTGGGATTTTAGCTTGAGTTTGACCAATCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
adh_short

Protein Analysis

309

Amino Acids

33.74

Weight (kDa)

9.22

Isoelectric Point (pI)

35.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KR PF08659 32 - 161 3.6e-07 KR domain
adh_short PF00106 33 - 240 1.1e-26 short chain dehydrogenase
adh_short_C2 PF13561 36 - 239 5.3e-14 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000238)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11410 AT4G11410 AT4G23420 AT4G23420 AT4G23420 AT4G23420 AT4G23420 AT4G23430 AT4G23430 AT4G23430 AT4G23430
fragaria_vesca FvH4_4g28972 FvH4_5g02640 FvH4_5g02640 FvH4_5g02640 FvH4_5g02640 FvH4_5g02640 FvH4_5g02640 FvH4_5g02640 FvH4_5g02641 FvH4_5g02642 FvH4_5g02643 FvH4_5g02644 FvH4_7g29900 FvH4_7g29900 FvH4_7g29900
malus_domestica MD00G1173300.v1.1 MD01G1205400.v1.1 MD06G1085800.v1.1 MD07G1274000.v1.1 MD07G1274200.v1.1 MD07G1274300.v1.1 MD07G1274400.v1.1 MD07G1274500.v1.1 MD07G1274700.v1.1 MD07G1274800.v1.1 MD14G1106700.v1.1 MD14G1106800.v1.1
prunus_persica Prupe.2G298000_v2.0.a1 Prupe.2G298000_v2.0.a1 Prupe.2G298000_v2.0.a1 Prupe.2G298000_v2.0.a1 Prupe.2G298000_v2.0.a1 Prupe.5G101600_v2.0.a1 Prupe.5G101900_v2.0.a1 Prupe.5G102000_v2.0.a1
pyrus_communis pycom01g21490 pycom06g08190 pycom06g08200 pycom07g25040 pycom07g25050 pycom07g25060 pycom07g25070 pycom07g25090
rosa_chinensis RchiOBHm_Chr1g0377861 RchiOBHm_Chr1g0377871 RchiOBHm_Chr7g0196741 RchiOBHm_Chr7g0197221 RchiOBHm_Chr7g0197291 RchiOBHm_Chr7g0197361 RchiOBHm_Chr7g0197421 RchiOBHm_Chr7g0197431 RchiOBHm_Chr7g0197441 RchiOBHm_Chr7g0197571
rosa_laevigata RLG00000003940 RLG00000003941 RLG00000003943 RLG00000003949 RLG00000003993 RLG00000026490 RLG00000026491
rosa_multiflora Rmu_co8456741.1_g000001 Rmu_sc0001083.1_g000032 Rmu_sc0001164.1_g000006 Rmu_sc0003263.1_g000014 Rmu_sc0004824.1_g000009 Rmu_sc0005659.1_g000027 Rmu_sc0010019.1_g000002 Rmu_sc0012104.1_g000004 Rmu_sc0013422.1_g000001 Rmu_sc0017623.1_g000003 Rmu_sc0018035.1_g000001
rosa_roxburghii Rroxscaffold_3G00258340 Rroxscaffold_3G00258480 Rroxscaffold_3G00258490 Rroxscaffold_3G00258990 Rroxscaffold_4G00280990
rosa_rugosa Rorug01G0405400 Rorug07G0036000 Rorug07G0036100 Rorug07G0038800 Rorug07G0038900 Rorug07G0039300 Rorug07G0039400 Rorug07G0039500 Rorug07G0039500 Rorug07G0041500 Rorug07G0041600 Rorug07G0041600
rosa_samantha Rh1BG382700 Rh1BG382800 Rh1CG396600 Rh1DG413700 Rh1DG413800 Rh2AG350300 Rh2DG223200 Rh2DG543200 Rh7AG159900 Rh7AG163400 Rh7AG163500 Rh7AG164300 Rh7AG164600 Rh7AG164700 Rh7AG165900 Rh7BG162100 Rh7BG165400 Rh7BG166100 Rh7BG166600 Rh7BG166700 Rh7BG166800 Rh7BG166900 Rh7BG167900 Rh7DG161200 Rh7DG164800 Rh7DG165500 Rh7DG165900 Rh7DG166000 Rh7DG166100
rosa_wichuraiana Rw1G037190 Rw1G037200 Rw2G016470 Rw2G028400 Rw7G013980 Rw7G014270 Rw7G014330 Rw7G014340 Rw7G014350 Rw7G014360 Rw7G014450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 155
Acc65I GGTACC 1 cut(s) 197
AccB1I GGYRCC 2 cut(s) 197, 642
AccII CGCG 1 cut(s) 139
AciI CCGC 2 cut(s) 819, 833
AcoI YGGCCR 1 cut(s) 514
AcsI RAATTY 3 cut(s) 290, 365, 540
AcyI GRCGYC 1 cut(s) 643
AfaI GTAC 1 cut(s) 199
AgsI TTSAA 3 cut(s) 96, 328, 370
AjnI CCWGG 1 cut(s) 688
AluBI AGCT 5 cut(s) 270, 373, 607, 634, 906
AluI AGCT 5 cut(s) 270, 373, 607, 634, 906
Alw26I GTCTC 3 cut(s) 125, 375, 883
Ama87I CYCGRG 1 cut(s) 378
AoxI GGCC 3 cut(s) 39, 316, 514
ApeKI GCWGC 3 cut(s) 296, 477, 770
ApoI RAATTY 3 cut(s) 290, 365, 540
AseI ATTAAT 1 cut(s) 558
Asp700I GAANNNNTTC 1 cut(s) 540
Asp718I GGTACC 1 cut(s) 197
AspLEI GCGC 3 cut(s) 139, 156, 645
AsuHPI GGTGA 1 cut(s) 833
AvaI CYCGRG 1 cut(s) 378
BaeI ACNNNNGTAYC 2 cut(s) 189, 222
BalI TGGCCA 1 cut(s) 516
BanI GGYRCC 2 cut(s) 197, 642
BbvI GCAGC 3 cut(s) 308, 489, 782
BccI CCATC 1 cut(s) 71
BciT130I CCWGG 1 cut(s) 690
BcoDI GTCTC 3 cut(s) 125, 375, 883
BfoI RGCGCY 1 cut(s) 646
BglI GCCNNNNNGGC 1 cut(s) 881
BglII AGATCT 1 cut(s) 262
BisI GCNGC 3 cut(s) 297, 478, 771
BlsI GCNGC 3 cut(s) 298, 479, 772
Bme1390I CCNGG 1 cut(s) 690
BmeT110I CYCGRG 1 cut(s) 378
BmiI GGNNCC 3 cut(s) 109, 199, 644
BmrFI CCNGG 1 cut(s) 690
BmsI GCATC 4 cut(s) 35, 181, 287, 862
BpuEI CTTGAG 5 cut(s) 51, 97, 632, 908, 928
BsaBI GATNNNNATC 1 cut(s) 526
BsaHI GRCGYC 1 cut(s) 643
Bse3DI GCAATG 2 cut(s) 149, 843
Bse8I GATNNNNATC 1 cut(s) 526
BseBI CCWGG 1 cut(s) 690
BseGI GGATG 2 cut(s) 278, 685
BseJI GATNNNNATC 1 cut(s) 526
BseMI GCAATG 2 cut(s) 149, 843
BseMII CTCAG 2 cut(s) 280, 875
BseXI GCAGC 3 cut(s) 308, 489, 782
Bsh1236I CGCG 1 cut(s) 139
BshFI GGCC 3 cut(s) 41, 318, 516
BshNI GGYRCC 2 cut(s) 197, 642
BsiHKCI CYCGRG 1 cut(s) 378
BsmAI GTCTC 3 cut(s) 125, 375, 883
BsnI GGCC 3 cut(s) 41, 318, 516
BsoBI CYCGRG 1 cut(s) 378
Bsp143I GATC 1 cut(s) 262
BspACI CCGC 2 cut(s) 819, 833
BspANI GGCC 3 cut(s) 41, 318, 516
BspCNI CTCAG 2 cut(s) 279, 874
BspFNI CGCG 1 cut(s) 139
BspLI GGNNCC 3 cut(s) 109, 199, 644
BspT107I GGYRCC 2 cut(s) 197, 642
BsrDI GCAATG 2 cut(s) 149, 843
BssMI GATC 1 cut(s) 262
BssNI GRCGYC 1 cut(s) 643
Bst2UI CCWGG 1 cut(s) 690
Bst6I CTCTTC 2 cut(s) 49, 687
BstACI GRCGYC 1 cut(s) 643
BstAPI GCANNNNNTGC 1 cut(s) 872
BstC8I GCNNGC 2 cut(s) 609, 624
BstDEI CTNAG 4 cut(s) 240, 266, 861, 885
BstF5I GGATG 2 cut(s) 278, 685
BstFNI CGCG 1 cut(s) 139
BstH2I RGCGCY 1 cut(s) 646
BstHHI GCGC 3 cut(s) 139, 156, 645
BstKTI GATC 1 cut(s) 265
BstMAI GTCTC 3 cut(s) 125, 375, 883
BstMBI GATC 1 cut(s) 262
BstMWI GCNNNNNNNGC 3 cut(s) 640, 872, 881
BstNI CCWGG 1 cut(s) 690
BstNSI RCATGY 2 cut(s) 626, 783
BstSCI CCNGG 1 cut(s) 688
BstUI CGCG 1 cut(s) 139
BstV1I GCAGC 3 cut(s) 308, 489, 782
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BsuRI GGCC 3 cut(s) 41, 318, 516
BtsCI GGATG 2 cut(s) 278, 685
BtsI GCAGTG 1 cut(s) 163
BtsIMutI CAGTG 2 cut(s) 163, 791
Cac8I GCNNGC 2 cut(s) 609, 624
CfoI GCGC 3 cut(s) 139, 156, 645
Csp6I GTAC 1 cut(s) 198
CviAII CATG 6 cut(s) 164, 253, 355, 454, 623, 780
CviQI GTAC 1 cut(s) 198
DdeI CTNAG 4 cut(s) 240, 266, 861, 885
DinI GGCGCC 1 cut(s) 644
DpnI GATC 1 cut(s) 264
DpnII GATC 1 cut(s) 262
EaeI YGGCCR 1 cut(s) 514
Eam1104I CTCTTC 2 cut(s) 49, 687
EarI CTCTTC 2 cut(s) 49, 687
Eco147I AGGCCT 1 cut(s) 318
Eco32I GATATC 1 cut(s) 724
Eco88I CYCGRG 1 cut(s) 378
EcoRI GAATTC 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 688
EcoRV GATATC 1 cut(s) 724
EcoT22I ATGCAT 1 cut(s) 717
EgeI GGCGCC 1 cut(s) 644
EheI GGCGCC 1 cut(s) 644
FaeI CATG 6 cut(s) 167, 256, 358, 457, 626, 783
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FatI CATG 6 cut(s) 163, 252, 354, 453, 622, 779
Fnu4HI GCNGC 3 cut(s) 297, 478, 771
FokI GGATG 2 cut(s) 265, 672
Fsp4HI GCNGC 3 cut(s) 297, 478, 771
FspI TGCGCA 1 cut(s) 155
GlaI GCGC 3 cut(s) 138, 155, 644
GluI GCNGC 3 cut(s) 297, 478, 771
HaeII RGCGCY 1 cut(s) 646
HaeIII GGCC 3 cut(s) 41, 318, 516
HhaI GCGC 3 cut(s) 139, 156, 645
Hin1I GRCGYC 1 cut(s) 643
Hin1II CATG 6 cut(s) 167, 256, 358, 457, 626, 783
Hin6I GCGC 3 cut(s) 137, 154, 643
HinP1I GCGC 3 cut(s) 137, 154, 643
HinfI GANTC 4 cut(s) 566, 599, 796, 891
HphI GGTGA 1 cut(s) 833
Hpy188III TCNNGA 4 cut(s) 378, 380, 468, 570
HpyAV CCTTC 2 cut(s) 329, 530
HpyCH4V TGCA 7 cut(s) 149, 296, 347, 404, 596, 622, 715
HpyF10VI GCNNNNNNNGC 3 cut(s) 640, 872, 881
HpyF3I CTNAG 4 cut(s) 240, 266, 861, 885
Hsp92I GRCGYC 1 cut(s) 643
Hsp92II CATG 6 cut(s) 167, 256, 358, 457, 626, 783
HspAI GCGC 3 cut(s) 137, 154, 643
KasI GGCGCC 1 cut(s) 642
KpnI GGTACC 1 cut(s) 201
Kzo9I GATC 1 cut(s) 262
LmnI GCTCC 3 cut(s) 23, 107, 767
Lsp1109I GCAGC 3 cut(s) 308, 489, 782
LweI GCATC 4 cut(s) 35, 181, 287, 862
MaeIII GTNAC 3 cut(s) 61, 100, 839
MalI GATC 1 cut(s) 264
MboI GATC 1 cut(s) 262
MboII GAAGA 5 cut(s) 36, 419, 431, 665, 674
MfeI CAATTG 2 cut(s) 96, 510
MflI RGATCY 1 cut(s) 262
MlsI TGGCCA 1 cut(s) 516
MluNI TGGCCA 1 cut(s) 516
Mly113I GGCGCC 1 cut(s) 643
MlyI GAGTC 1 cut(s) 885
MnlI CCTC 5 cut(s) 52, 121, 344, 478, 656
Mox20I TGGCCA 1 cut(s) 516
Mph1103I ATGCAT 1 cut(s) 717
MroXI GAANNNNTTC 1 cut(s) 540
MscI TGGCCA 1 cut(s) 516
MseI TTAA 6 cut(s) 207, 222, 339, 498, 558, 807
MslI CAYNNNNRTG 1 cut(s) 627
Msp20I TGGCCA 1 cut(s) 516
MspR9I CCNGG 1 cut(s) 690
MunI CAATTG 2 cut(s) 96, 510
MvaI CCWGG 1 cut(s) 690
MvnI CGCG 1 cut(s) 139
MwoI GCNNNNNNNGC 3 cut(s) 640, 872, 881
NarI GGCGCC 1 cut(s) 643
NdeII GATC 1 cut(s) 262
NlaIII CATG 6 cut(s) 167, 256, 358, 457, 626, 783
NlaIV GGNNCC 3 cut(s) 109, 199, 644
NmeAIII GCCGAG 1 cut(s) 17
NsbI TGCGCA 1 cut(s) 155
NsiI ATGCAT 1 cut(s) 717
NspI RCATGY 2 cut(s) 626, 783
PaeI GCATGC 1 cut(s) 626
PaeR7I CTCGAG 1 cut(s) 378
PceI AGGCCT 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 540
PfeI GAWTC 3 cut(s) 566, 599, 796
PfoI TCCNGGA 1 cut(s) 688
PkrI GCNGC 3 cut(s) 298, 479, 772
PleI GAGTC 1 cut(s) 885
PluTI GGCGCC 1 cut(s) 646
PpsI GAGTC 1 cut(s) 885
PshBI ATTAAT 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 688
PspGI CCWGG 1 cut(s) 688
PspN4I GGNNCC 3 cut(s) 109, 199, 644
PsuI RGATCY 1 cut(s) 262
RsaI GTAC 1 cut(s) 199
RsaNI GTAC 1 cut(s) 198
RseI CAYNNNNRTG 1 cut(s) 627
SaqAI TTAA 6 cut(s) 207, 222, 339, 498, 558, 807
SatI GCNGC 3 cut(s) 297, 478, 771
Sau3AI GATC 1 cut(s) 262
SchI GAGTC 1 cut(s) 885
ScrFI CCNGG 1 cut(s) 690
SetI ASST 7 cut(s) 272, 375, 421, 609, 636, 807, 908
SfaNI GCATC 4 cut(s) 35, 181, 287, 862
SfoI GGCGCC 1 cut(s) 644
Sfr274I CTCGAG 1 cut(s) 378
SlaI CTCGAG 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 627
SmlI CTYRAG 6 cut(s) 66, 112, 378, 647, 907, 923
SmoI CTYRAG 6 cut(s) 66, 112, 378, 647, 907, 923
SphI GCATGC 1 cut(s) 626
SseBI AGGCCT 1 cut(s) 318
SsiI CCGC 2 cut(s) 819, 833
SspDI GGCGCC 1 cut(s) 642
SspI AATATT 1 cut(s) 709
StuI AGGCCT 1 cut(s) 318
StyD4I CCNGG 1 cut(s) 688
TaqI TCGA 2 cut(s) 379, 396
TfiI GAWTC 3 cut(s) 566, 599, 796
Tru1I TTAA 6 cut(s) 207, 222, 339, 498, 558, 807
Tru9I TTAA 6 cut(s) 207, 222, 339, 498, 558, 807
TscAI CASTG 2 cut(s) 163, 798
TseI GCWGC 3 cut(s) 296, 477, 770
TspDTI ATGAA 3 cut(s) 152, 470, 674
TspGWI ACGGA 2 cut(s) 271, 716
TspRI CASTG 2 cut(s) 163, 798
VspI ATTAAT 1 cut(s) 558
XapI RAATTY 3 cut(s) 290, 365, 540
XceI RCATGY 2 cut(s) 626, 783
XcmI CCANNNNNNNNNTGG 1 cut(s) 784
XhoI CTCGAG 1 cut(s) 378
XmnI GAANNNNTTC 1 cut(s) 540
Zsp2I ATGCAT 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.