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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 23 |
| ApoI | RAATTY | 1 cut(s) | 23 |
| BccI | CCATC | 1 cut(s) | 74 |
| BsiSI | CCGG | 1 cut(s) | 118 |
| Bst4CI | ACNGT | 1 cut(s) | 144 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 114 |
| CviAII | CATG | 1 cut(s) | 158 |
| CviJI | RGCY | 2 cut(s) | 59, 117 |
| CviKI_1 | RGCY | 2 cut(s) | 59, 117 |
| FaeI | CATG | 1 cut(s) | 161 |
| FaiI | YATR | 2 cut(s) | 152, 159 |
| FatI | CATG | 1 cut(s) | 157 |
| HapII | CCGG | 1 cut(s) | 118 |
| Hin1II | CATG | 1 cut(s) | 161 |
| HpaII | CCGG | 1 cut(s) | 118 |
| Hpy188I | TCNGA | 1 cut(s) | 21 |
| HpyCH4III | ACNGT | 1 cut(s) | 144 |
| HpyCH4V | TGCA | 1 cut(s) | 108 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 114 |
| Hsp92II | CATG | 1 cut(s) | 161 |
| LpnPI | CCDG | 3 cut(s) | 21, 125, 131 |
| MluCI | AATT | 1 cut(s) | 23 |
| MnlI | CCTC | 1 cut(s) | 43 |
| MseI | TTAA | 1 cut(s) | 51 |
| MspI | CCGG | 1 cut(s) | 118 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 114 |
| NlaIII | CATG | 1 cut(s) | 161 |
| SaqAI | TTAA | 1 cut(s) | 51 |
| SetI | ASST | 1 cut(s) | 135 |
| SgeI | CNNG | 4 cut(s) | 48, 124, 130, 146 |
| Sse9I | AATT | 1 cut(s) | 23 |
| TaaI | ACNGT | 1 cut(s) | 144 |
| TasI | AATT | 1 cut(s) | 23 |
| Tru1I | TTAA | 1 cut(s) | 51 |
| Tru9I | TTAA | 1 cut(s) | 51 |
| TspDTI | ATGAA | 1 cut(s) | 153 |
| XapI | RAATTY | 1 cut(s) | 23 |