Rorug01G0231200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000500
Physical Location & Seq
Forward (+)
33305012 .. 33312724
7713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0231200.1

Sequence Viewer

Length: 2262 bp
ATGCCCCGCTTCCCTCGCTTCTCCATCCCGCGCCTCCTCTACTCCCTCCACACCTCCAAAACGACGCCGTCCCCGTCCCCGTCCTCCTCTCTCCTCCTCGGCTCCTTCCACCTCCGCCAATTCTCGGCCGGCAACCTCGCACGTGCCAAGGACGACAAGGAGCTATGGTGGACGGACGTGATGGCCAGGCTCCGCAACATCGGAATCTCGGCCCATATCGACTCCGGCAAGACCACTCTCACCGAGAGAATTCTCTACTACACCGGAAAGATCCACGAGATTCACGAGGTCAGAGGCCGAGACGGCGTCGGCGCCAAAATGGACTCCATGGATTTGGAGAGAGAGAAGGGAATTACCATTCAGTCCGCTGCCACTTACTGCACTTGGAATGGTTATCAGATTAACATAATTGACACCCCTGGTCACGTTGATTTCACCATTGAGGTTGAGAGAGCTTTGCGTGTTCTTGATGGAGCCATCCTTGTCCTTTGTAGCGTTGGTGGTGTACAGAGTCAGTCAATTACTGTTGATCGGCAAATGAAAAGATATGAGGTTCCAAGAATTGCATTTATAAACAAACTTGATCGAATGGGAGCAGATCCATGGAAAGTTCTGAATCAGATGAGGTCTAAGCTCCGGCATCATGCTGCTGCATTGCAACTTCCTATTGGGTTGGAAGATGATTTTAAGGGTCTTATTGATCTTGTGCAGTTAAAAGCTTTGTATTTTCATGGTTCTAGCGGTCAGGAAATTGTCATTGAAGACATTCCTTCTGATATGGAGGCGTTAGTCGCAGAAAAAAGGCGTGAGCTAATTGAAATTGTTTCTGAAGTTGATGACCAACTTGCTGAAGCATTTCTTGAAGATGTGCCCATTACACCTACTGATCTGGAGGAAGCTGTGCGAAGGGCTACTATCGCAAGGAAATTTATACCTGTGTTCATGGGTAGTGCATTTAAAAACAAGGGTGTACAACCACTTTTGAATGGTGTACTTGGTTATTTGCCTTGTCCATCTGAAGTCAGTAACTATGCTCTTGACCAGACTAAGGATGAAGAGAAGGTTATATTAGGTGGAACTCCAGATGGACCCCTTGTAGCATTGGCCTTTAAATTGGAGGAAGGGCGTTTTGGTCAGTTAACCTTTCTAAGAATCTATGAAGGTGTTATACGGAAGGGTGATTTCATTTTTAACATTAACACTGGCAAGAAGATTAAGGTTCCTCGCTTGGTCCGGATGCATTCTGATGAGATGGAGGATATTCAAGAGGCACATGCTGGACAAATAGTTGCTGTATTTGGTGTGGATTGTGCATCAGGAGATACATTTACTGACGGTTCAGTTAAATATACCATGACTTCTATGAATGTCCCTGAGCCAGTGATGTCATTAGCTGTTCAGCCAGTTTCAAAAGATTCTGGAGGACAATTTTCGAAGGCTTTGAATCGTTTTCAGAAAGAAGATCCTACCTTCCGTGTTGGCTTGGACCCTGAGAGCGGACAGACAATCATATCAGGAATGGGAGAGCTGCATTTGGAAGTTTATGTTGAACGCATTCGGAGAGAATATAAGGTTGATGCAACTGTTGGAAAGCCTCGTGTAAACTTCAGAGAGACTGTTACTCAACGTGCCGACTTTGATTATTTACATAAAAAGCAATCTGGAGGACAGGGTCAATATGGGCGAGTAACTGGATTTATCGAACCGCTGCCTGCAGGATCAGCGACAAAGTTTGAATTTGAAAACAGTATTGTGGGACAAGCTATACCATCAAATTTTATCCCAGCTATTGAGAAGGGTTTTAAAGAAGCAGCCAATTCGGGCTCATTAATTGGCCATCCAGTTGAAAATGTTCGCATTGTTTTAACTGATGGTGCTTCCCATGCTGTGGATTCTAGTGAACTTGCCTTCAAATTAGCTGCAATATATGCCTTTAGAAAGTGCTATACGGTGGCAAAGCCGGTGATACTGGAGCCTGTTATGTTGGTGGAGTTGAAAGTACCTACAGAATTTCAGGGCACTGTTGCTGGTGATATTAACAAGAGAAAAGGTGTCATTATTGGAAATGATCAGGAAGGGGATGACTCTGTTATTACGGCCCAAGTCCCTCTGAACAATATGTTTGGCTATTCGACGTCTCTTCGATCAATGACACAGGGAAAAGGCGAATTCACAATGGAGTACAAAGAGCACTCTCCCGTTTCTCATGATGTGCAGGCGCAATTAGTAAACAATTACAAGGGACCCAATGCCACTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

753

Amino Acids

83.35

Weight (kDa)

5.95

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 63 - 336 8.9e-61 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 377 - 445 8.4e-11 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 378 - 444 4.5e-14 Elongation factor Tu domain 2
EFG_III PF14492 457 - 531 2.6e-29 Elongation Factor G, domain III
EFG_IV PF03764 532 - 652 6.2e-38 Elongation factor G, domain IV
EFG_C PF00679 656 - 741 7.7e-26 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21520
fragaria_vesca FvH4_2g24233 FvH4_2g28070 FvH4_2g28080 FvH4_2g28100 FvH4_2g28120 FvH4_2g28140 FvH4_2g28150 FvH4_2g28160 FvH4_2g28170 FvH4_2g28200 FvH4_2g28210 FvH4_2g28211 FvH4_2g28220 FvH4_2g28230
prunus_persica Prupe.1G487200_v2.0.a1 Prupe.3G116400_v2.0.a1 Prupe.8G205900_v2.0.a1 Prupe.8G206000_v2.0.a1 Prupe.8G206100_v2.0.a1 Prupe.8G206300_v2.0.a1
pyrus_communis pycom05g16480 pycom05g16510 pycom05g16520 pycom05g16540 pycom10g14500 pycom10g14510 pycom10g14540 pycom12g16800
rosa_chinensis RchiOBHm_Chr6g0297031 RchiOBHm_Chr6g0297041
rosa_laevigata RLG00000011633 RLG00000011635 RLG00000011636 RLG00000011637 RLG00000011638 RLG00000011643 RLG00000011644
rosa_roxburghii Rroxscaffold_2G00150260 Rroxscaffold_4G00302450 Rroxscaffold_7G00170860 Rroxscaffold_7G00170880 Rroxscaffold_7G00170890 Rroxscaffold_7G00170910 Rroxscaffold_7G00170920 Rroxscaffold_7G00170950 Rroxscaffold_7G00171000
rosa_rugosa Rorug01G0231200 Rorug06G0267900 Rorug06G0268000 Rorug06G0268100 Rorug06G0268200 Rorug06G0268600 Rorug06G0268700 Rorug06G0268700 Rorug06G0268700 Rorug06G0269100
rosa_samantha Rh6AG378600 Rh6AG378700 Rh6AG379000 Rh6AG379200 Rh6AG379300 Rh6AG379600 Rh6AG379900 Rh6AG380100 Rh6AG380300 Rh6BG386600 Rh6BG386700 Rh6BG386800 Rh6BG387300 Rh6BG387600 Rh6BG388100 Rh6BG388300 Rh6CG392500 Rh6CG392600 Rh6CG392700 Rh6CG393000 Rh6CG393100 Rh6CG393200 Rh6CG393300 Rh6CG393500 Rh6CG393700 Rh6DG379200 Rh6DG379400 Rh6DG379600 Rh6DG380100 Rh6DG380200 Rh6DG380300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 572
AatII GACGTC 1 cut(s) 2138
AccB1I GGYRCC 1 cut(s) 311
AccB7I CCANNNNNTGG 1 cut(s) 1888
AccBSI CCGCTC 1 cut(s) 1497
AccII CGCG 1 cut(s) 31
AccIII TCCGGA 1 cut(s) 1233
AciI CCGC 8 cut(s) 7, 29, 115, 193, 366, 741, 1497, 1706
AclWI GGATC 4 cut(s) 265, 593, 1457, 1726
AcoI YGGCCR 3 cut(s) 126, 183, 1834
AcsI RAATTY 6 cut(s) 249, 926, 1736, 1774, 2009, 2168
AcuI CTGAAG 4 cut(s) 849, 870, 1038, 1591
AcvI CACGTG 1 cut(s) 143
AcyI GRCGYC 4 cut(s) 65, 306, 312, 2135
AfaI GTAC 5 cut(s) 507, 972, 993, 2001, 2183
AfiI CCNNNNNNNGG 4 cut(s) 124, 1048, 1496, 1888
AjiI CACGTC 1 cut(s) 178
AjnI CCWGG 2 cut(s) 185, 418
AjuI GAANNNNNNNTTGG 2 cut(s) 1113, 1145
Alw21I GWGCWC 1 cut(s) 2193
Alw26I GTCTC 3 cut(s) 294, 1607, 2142
AlwI GGATC 4 cut(s) 265, 593, 1457, 1726
Aor13HI TCCGGA 1 cut(s) 1233
AoxI GGCC 7 cut(s) 126, 183, 210, 295, 1104, 1834, 2097
ApeKI GCWGC 7 cut(s) 368, 647, 650, 1528, 1708, 1811, 1919
ApoI RAATTY 6 cut(s) 249, 926, 1736, 1774, 2009, 2168
AseI ATTAAT 1 cut(s) 1829
Asp700I GAANNNNTTC 4 cut(s) 765, 855, 1554, 1851
AspLEI GCGC 3 cut(s) 33, 314, 2221
AspS9I GGNCC 6 cut(s) 211, 1088, 1231, 1486, 2098, 2243
AsuHPI GGTGA 5 cut(s) 232, 427, 1190, 1975, 2042
AsuII TTCGAA 1 cut(s) 1433
AvaII GGWCC 4 cut(s) 1088, 1231, 1486, 2243
BaeGI GKGCMC 2 cut(s) 873, 2021
BalI TGGCCA 2 cut(s) 185, 1836
BanI GGYRCC 1 cut(s) 311
BanII GRGCYC 1 cut(s) 1826
BarI GAAGNNNNNNTAC 2 cut(s) 1152, 1184
BauI CACGAG 3 cut(s) 275, 284, 1596
BbrPI CACGTG 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 768
Bbv12I GWGCWC 1 cut(s) 2193
BbvI GCAGC 7 cut(s) 355, 634, 637, 1515, 1695, 1823, 1906
BceAI ACGGC 3 cut(s) 52, 319, 2112
BcgI CGANNNNNNTGC 2 cut(s) 1800, 1834
BciT130I CCWGG 2 cut(s) 187, 420
BclI TGATCA 1 cut(s) 2068
BcoDI GTCTC 3 cut(s) 294, 1607, 2142
BfaI CTAG 2 cut(s) 738, 1896
BfmI CTRYAG 2 cut(s) 1713, 2004
BfoI RGCGCY 1 cut(s) 315
BglI GCCNNNNNGGC 1 cut(s) 303
BisI GCNGC 7 cut(s) 369, 648, 651, 1529, 1709, 1812, 1920
BlsI GCNGC 7 cut(s) 370, 649, 652, 1530, 1710, 1813, 1921
Bme1390I CCNGG 2 cut(s) 187, 420
Bme18I GGWCC 4 cut(s) 1088, 1231, 1486, 2243
BmgBI CACGTC 1 cut(s) 178
BmgT120I GGNCC 6 cut(s) 211, 1088, 1231, 1486, 2098, 2243
BmrFI CCNGG 2 cut(s) 187, 420
BmsI GCATC 4 cut(s) 649, 1227, 1322, 1567
BpiI GAAGAC 1 cut(s) 768
BpmI CTGGAG 5 cut(s) 911, 1065, 1440, 1683, 1991
Bpu10I CCTNAGC 1 cut(s) 1374
Bpu14I TTCGAA 1 cut(s) 1433
BsaAI YACGTR 1 cut(s) 143
BsaHI GRCGYC 4 cut(s) 65, 306, 312, 2135
BsaJI CCNNGG 5 cut(s) 97, 147, 327, 418, 602
BsaWI WCCGGW 2 cut(s) 263, 1233
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 1048, 1496, 1888
Bse118I RCCGGY 2 cut(s) 128, 1960
Bse1I ACTGG 6 cut(s) 1207, 1379, 1403, 1696, 1841, 1974
Bse3DI GCAATG 1 cut(s) 653
BseAI TCCGGA 1 cut(s) 1233
BseBI CCWGG 2 cut(s) 187, 420
BseDI CCNNGG 5 cut(s) 97, 147, 327, 418, 602
BseGI GGATG 6 cut(s) 24, 477, 1057, 1242, 1837, 2086
BseLI CCNNNNNNNGG 4 cut(s) 124, 1048, 1496, 1888
BseMI GCAATG 1 cut(s) 653
BseMII CTCAG 2 cut(s) 1365, 1482
BseNI ACTGG 6 cut(s) 1207, 1379, 1403, 1696, 1841, 1974
BseRI GAGGAG 4 cut(s) 26, 76, 83, 86
BseSI GKGCMC 2 cut(s) 873, 2021
BseX3I CGGCCG 1 cut(s) 126
BseXI GCAGC 7 cut(s) 355, 634, 637, 1515, 1695, 1823, 1906
BseYI CCCAGC 1 cut(s) 1783
BsgI GTGCAG 3 cut(s) 364, 728, 2234
Bsh1236I CGCG 1 cut(s) 31
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 7 cut(s) 128, 185, 212, 297, 1106, 1836, 2099
BshNI GGYRCC 1 cut(s) 311
BsiEI CGRYCG 1 cut(s) 129
BsiHKAI GWGCWC 1 cut(s) 2193
BsiSI CCGG 6 cut(s) 129, 225, 264, 637, 1234, 1961
BslFI GGGAC 6 cut(s) 55, 61, 1355, 1770, 2090, 2256
BslI CCNNNNNNNGG 4 cut(s) 124, 1048, 1496, 1888
BsmAI GTCTC 3 cut(s) 294, 1607, 2142
BsmBI CGTCTC 2 cut(s) 294, 2142
BsmFI GGGAC 6 cut(s) 55, 61, 1355, 1770, 2090, 2256
BsmI GAATGC 2 cut(s) 1240, 1554
BsnI GGCC 7 cut(s) 128, 185, 212, 297, 1106, 1836, 2099
Bsp119I TTCGAA 1 cut(s) 1433
Bsp1286I GDGCHC 4 cut(s) 873, 1826, 2021, 2193
Bsp13I TCCGGA 1 cut(s) 1233
Bsp1407I TGTACA 2 cut(s) 505, 970
Bsp19I CCATGG 2 cut(s) 327, 602
BspACI CCGC 8 cut(s) 7, 29, 115, 193, 366, 741, 1497, 1706
BspANI GGCC 7 cut(s) 128, 185, 212, 297, 1106, 1836, 2099
BspCNI CTCAG 2 cut(s) 1366, 1483
BspEI TCCGGA 1 cut(s) 1233
BspFNI CGCG 1 cut(s) 31
BspHI TCATGA 1 cut(s) 2206
BspMAI CTGCAG 1 cut(s) 1717
BspPI GGATC 4 cut(s) 265, 593, 1457, 1726
BspT104I TTCGAA 1 cut(s) 1433
BspT107I GGYRCC 1 cut(s) 311
BsrBI CCGCTC 1 cut(s) 1497
BsrDI GCAATG 1 cut(s) 653
BsrFI RCCGGY 2 cut(s) 128, 1960
BsrGI TGTACA 2 cut(s) 505, 970
BsrI ACTGG 6 cut(s) 1207, 1379, 1403, 1696, 1841, 1974
BssAI RCCGGY 2 cut(s) 128, 1960
BssECI CCNNGG 5 cut(s) 97, 147, 327, 418, 602
BssNI GRCGYC 4 cut(s) 65, 306, 312, 2135
BssSI CACGAG 3 cut(s) 275, 284, 1596
BssT1I CCWWGG 3 cut(s) 147, 327, 602
Bst2BI CACGAG 3 cut(s) 275, 284, 1596
Bst2UI CCWGG 2 cut(s) 187, 420
Bst4CI ACNGT 7 cut(s) 526, 1337, 1585, 1618, 1748, 1951, 2023
Bst6I CTCTTC 2 cut(s) 1050, 2145
BstACI GRCGYC 4 cut(s) 65, 306, 312, 2135
BstAPI GCANNNNNTGC 1 cut(s) 1928
BstAUI TGTACA 2 cut(s) 505, 970
BstBAI YACGTR 1 cut(s) 143
BstBI TTCGAA 1 cut(s) 1433
BstC8I GCNNGC 3 cut(s) 130, 1713, 2217
BstDEI CTNAG 5 cut(s) 630, 1047, 1148, 1374, 1491
BstDSI CCRYGG 2 cut(s) 327, 602
BstF5I GGATG 6 cut(s) 24, 477, 1057, 1242, 1837, 2086
BstFNI CGCG 1 cut(s) 31
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 3 cut(s) 33, 314, 2221
BstMAI GTCTC 3 cut(s) 294, 1607, 2142
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 7 cut(s) 15, 303, 791, 917, 1721, 1883, 1928
BstNI CCWGG 2 cut(s) 187, 420
BstNSI RCATGY 1 cut(s) 1277
BstSCI CCNGG 2 cut(s) 185, 418
BstSFI CTRYAG 2 cut(s) 1713, 2004
BstSLI GKGCMC 2 cut(s) 873, 2021
BstUI CGCG 1 cut(s) 31
BstV1I GCAGC 7 cut(s) 355, 634, 637, 1515, 1695, 1823, 1906
BstV2I GAAGAC 1 cut(s) 768
BstX2I RGATCY 3 cut(s) 270, 598, 1462
BstXI CCANNNNNNTGG 1 cut(s) 334
BstYI RGATCY 3 cut(s) 270, 598, 1462
BstZI CGGCCG 1 cut(s) 126
BsuRI GGCC 7 cut(s) 128, 185, 212, 297, 1106, 1836, 2099
BtgI CCRYGG 2 cut(s) 327, 602
BtrI CACGTC 1 cut(s) 178
BtsCI GGATG 6 cut(s) 24, 477, 1057, 1242, 1837, 2086
BtsIMutI CAGTG 4 cut(s) 1200, 1386, 2019, 2253
Cac8I GCNNGC 3 cut(s) 130, 1713, 2217
CciI TCATGA 1 cut(s) 2206
CfoI GCGC 3 cut(s) 33, 314, 2221
Cfr10I RCCGGY 2 cut(s) 128, 1960
Cfr13I GGNCC 6 cut(s) 211, 1088, 1231, 1486, 2098, 2243
CseI GACGC 2 cut(s) 73, 295
Csp6I GTAC 5 cut(s) 506, 971, 992, 2000, 2182
CviAII CATG 9 cut(s) 328, 603, 644, 731, 943, 1274, 1354, 1883, 2207
CviQI GTAC 5 cut(s) 506, 971, 992, 2000, 2182
DdeI CTNAG 5 cut(s) 630, 1047, 1148, 1374, 1491
DinI GGCGCC 1 cut(s) 313
DraI TTTAAA 3 cut(s) 958, 1111, 1804
EaeI YGGCCR 3 cut(s) 126, 183, 1834
EagI CGGCCG 1 cut(s) 126
Eam1104I CTCTTC 2 cut(s) 1050, 2145
EarI CTCTTC 2 cut(s) 1050, 2145
EciI GGCGGA 1 cut(s) 104
EclXI CGGCCG 1 cut(s) 126
Eco130I CCWWGG 3 cut(s) 147, 327, 602
Eco24I GRGCYC 1 cut(s) 1826
Eco47I GGWCC 4 cut(s) 1088, 1231, 1486, 2243
Eco52I CGGCCG 1 cut(s) 126
Eco57I CTGAAG 4 cut(s) 849, 870, 1038, 1591
Eco72I CACGTG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 2243
EcoRI GAATTC 2 cut(s) 249, 2168
EcoRII CCWGG 2 cut(s) 185, 418
EcoT14I CCWWGG 3 cut(s) 147, 327, 602
EcoT22I ATGCAT 1 cut(s) 1242
EcoT38I GRGCYC 1 cut(s) 1826
EgeI GGCGCC 1 cut(s) 313
EheI GGCGCC 1 cut(s) 313
ErhI CCWWGG 3 cut(s) 147, 327, 602
Esp3I CGTCTC 2 cut(s) 294, 2142
FaeI CATG 9 cut(s) 331, 606, 647, 734, 946, 1277, 1357, 1886, 2210
FalI AAGNNNNNCTT 2 cut(s) 843, 875
FaqI GGGAC 6 cut(s) 55, 61, 1355, 1770, 2090, 2256
FatI CATG 9 cut(s) 327, 602, 643, 730, 942, 1273, 1353, 1882, 2206
FauI CCCGC 2 cut(s) 14, 36
FbaI TGATCA 1 cut(s) 2068
Fnu4HI GCNGC 7 cut(s) 369, 648, 651, 1529, 1709, 1812, 1920
FokI GGATG 6 cut(s) 11, 464, 1064, 1249, 1824, 2093
FriOI GRGCYC 1 cut(s) 1826
Fsp4HI GCNGC 7 cut(s) 369, 648, 651, 1529, 1709, 1812, 1920
FspBI CTAG 2 cut(s) 738, 1896
GlaI GCGC 3 cut(s) 32, 313, 2220
GluI GCNGC 7 cut(s) 369, 648, 651, 1529, 1709, 1812, 1920
GsaI CCCAGC 1 cut(s) 1787
GsuI CTGGAG 5 cut(s) 911, 1065, 1440, 1683, 1991
HaeII RGCGCY 1 cut(s) 315
HaeIII GGCC 7 cut(s) 128, 185, 212, 297, 1106, 1836, 2099
HapII CCGG 6 cut(s) 129, 225, 264, 637, 1234, 1961
HgaI GACGC 2 cut(s) 73, 295
HhaI GCGC 3 cut(s) 33, 314, 2221
Hin1I GRCGYC 4 cut(s) 65, 306, 312, 2135
Hin1II CATG 9 cut(s) 331, 606, 647, 734, 946, 1277, 1357, 1886, 2210
Hin6I GCGC 3 cut(s) 31, 312, 2219
HinP1I GCGC 3 cut(s) 31, 312, 2219
HincII GTYRAC 1 cut(s) 1140
HindII GTYRAC 1 cut(s) 1140
HindIII AAGCTT 1 cut(s) 717
HpaI GTTAAC 1 cut(s) 1140
HpaII CCGG 6 cut(s) 129, 225, 264, 637, 1234, 1961
HphI GGTGA 5 cut(s) 232, 427, 1190, 1975, 2042
Hpy166II GTNNAC 8 cut(s) 171, 506, 971, 992, 1140, 1603, 1901, 2230
Hpy8I GTNNAC 8 cut(s) 171, 506, 971, 992, 1140, 1603, 1901, 2230
Hpy99I CGWCG 3 cut(s) 67, 311, 2137
HpyCH4III ACNGT 7 cut(s) 526, 1337, 1585, 1618, 1748, 1951, 2023
HpyCH4IV ACGT 5 cut(s) 142, 177, 426, 1627, 2135
HpyF10VI GCNNNNNNNGC 7 cut(s) 15, 303, 791, 917, 1721, 1883, 1928
HpyF3I CTNAG 5 cut(s) 630, 1047, 1148, 1374, 1491
HpySE526I ACGT 5 cut(s) 142, 177, 426, 1627, 2135
Hsp92I GRCGYC 4 cut(s) 65, 306, 312, 2135
Hsp92II CATG 9 cut(s) 331, 606, 647, 734, 946, 1277, 1357, 1886, 2210
HspAI GCGC 3 cut(s) 31, 312, 2219
KasI GGCGCC 1 cut(s) 311
KflI GGGWCCC 1 cut(s) 2243
Kpn2I TCCGGA 1 cut(s) 1233
KroI GCCGGC 1 cut(s) 128
KroNI GCCGGC 1 cut(s) 130
Ksp22I TGATCA 1 cut(s) 2068
KspAI GTTAAC 1 cut(s) 1140
LmnI GCTCC 7 cut(s) 107, 160, 195, 473, 593, 639, 1972
Lsp1109I GCAGC 7 cut(s) 355, 634, 637, 1515, 1695, 1823, 1906
LweI GCATC 4 cut(s) 649, 1227, 1322, 1567
MaeI CTAG 2 cut(s) 738, 1896
MaeII ACGT 5 cut(s) 142, 177, 426, 1627, 2135
MaeIII GTNAC 4 cut(s) 422, 1025, 1618, 1687
MbiI CCGCTC 1 cut(s) 1497
MboII GAAGA 7 cut(s) 689, 773, 875, 1067, 1222, 1472, 2132
MflI RGATCY 3 cut(s) 270, 598, 1462
MhlI GDGCHC 4 cut(s) 873, 1826, 2021, 2193
MlsI TGGCCA 2 cut(s) 185, 1836
MluNI TGGCCA 2 cut(s) 185, 1836
Mly113I GGCGCC 1 cut(s) 312
MlyI GAGTC 4 cut(s) 215, 317, 520, 2078
MmeI TCCRAC 2 cut(s) 654, 1567
Mox20I TGGCCA 2 cut(s) 185, 1836
Mph1103I ATGCAT 1 cut(s) 1242
MroI TCCGGA 1 cut(s) 1233
MroNI GCCGGC 1 cut(s) 128
MroXI GAANNNNTTC 4 cut(s) 765, 855, 1554, 1851
MscI TGGCCA 2 cut(s) 185, 1836
MslI CAYNNNNRTG 1 cut(s) 1245
Msp20I TGGCCA 2 cut(s) 185, 1836
MspA1I CMGCKG 2 cut(s) 368, 1708
MspI CCGG 6 cut(s) 129, 225, 264, 637, 1234, 1961
MspR9I CCNGG 2 cut(s) 187, 420
Mva1269I GAATGC 2 cut(s) 1240, 1554
MvaI CCWGG 2 cut(s) 187, 420
MvnI CGCG 1 cut(s) 31
MwoI GCNNNNNNNGC 7 cut(s) 15, 303, 791, 917, 1721, 1883, 1928
NaeI GCCGGC 1 cut(s) 130
NarI GGCGCC 1 cut(s) 312
NcoI CCATGG 2 cut(s) 327, 602
NgoMIV GCCGGC 1 cut(s) 128
NlaIII CATG 9 cut(s) 331, 606, 647, 734, 946, 1277, 1357, 1886, 2210
NmeAIII GCCGAG 4 cut(s) 78, 104, 188, 323
NmuCI GTSAC 1 cut(s) 422
NsiI ATGCAT 1 cut(s) 1242
NspI RCATGY 1 cut(s) 1277
NspV TTCGAA 1 cut(s) 1433
PagI TCATGA 1 cut(s) 2206
PcsI WCGNNNNNNNCGW 2 cut(s) 71, 282
PctI GAATGC 2 cut(s) 1240, 1554
PdiI GCCGGC 1 cut(s) 130
PdmI GAANNNNTTC 4 cut(s) 765, 855, 1554, 1851
PfeI GAWTC 7 cut(s) 204, 280, 616, 1152, 1415, 1444, 1892
PflFI GACNNNGTC 3 cut(s) 67, 305, 1671
PflMI CCANNNNNTGG 1 cut(s) 1888
PkrI GCNGC 7 cut(s) 370, 649, 652, 1530, 1710, 1813, 1921
PleI GAGTC 4 cut(s) 215, 317, 519, 2078
PluTI GGCGCC 1 cut(s) 315
PmaCI CACGTG 1 cut(s) 143
PmlI CACGTG 1 cut(s) 143
PpsI GAGTC 4 cut(s) 215, 317, 519, 2078
Ppu21I YACGTR 1 cut(s) 143
PpuMI RGGWCCY 1 cut(s) 2243
PshBI ATTAAT 1 cut(s) 1829
PsiI TTATAA 1 cut(s) 572
Psp5II RGGWCCY 1 cut(s) 2243
Psp6I CCWGG 2 cut(s) 185, 418
PspCI CACGTG 1 cut(s) 143
PspFI CCCAGC 1 cut(s) 1783
PspGI CCWGG 2 cut(s) 185, 418
PspPI GGNCC 6 cut(s) 211, 1088, 1231, 1486, 2098, 2243
PspPPI RGGWCCY 1 cut(s) 2243
PstI CTGCAG 1 cut(s) 1717
PsuI RGATCY 3 cut(s) 270, 598, 1462
PsyI GACNNNGTC 3 cut(s) 67, 305, 1671
RsaI GTAC 5 cut(s) 507, 972, 993, 2001, 2183
RsaNI GTAC 5 cut(s) 506, 971, 992, 2000, 2182
RseI CAYNNNNRTG 1 cut(s) 1245
SatI GCNGC 7 cut(s) 369, 648, 651, 1529, 1709, 1812, 1920
Sau96I GGNCC 6 cut(s) 211, 1088, 1231, 1486, 2098, 2243
SbfI CCTGCAGG 1 cut(s) 1717
SchI GAGTC 4 cut(s) 215, 317, 520, 2078
ScrFI CCNGG 2 cut(s) 187, 420
SdaI CCTGCAGG 1 cut(s) 1717
SduI GDGCHC 4 cut(s) 873, 1826, 2021, 2193
SfaNI GCATC 4 cut(s) 649, 1227, 1322, 1567
SfcI CTRYAG 2 cut(s) 1713, 2004
SfoI GGCGCC 1 cut(s) 313
SfuI TTCGAA 1 cut(s) 1433
SinI GGWCC 4 cut(s) 1088, 1231, 1486, 2243
SmiMI CAYNNNNRTG 1 cut(s) 1245
Sse8387I CCTGCAGG 1 cut(s) 1717
SsiI CCGC 8 cut(s) 7, 29, 115, 193, 366, 741, 1497, 1706
SspDI GGCGCC 1 cut(s) 311
SspMI CTAG 2 cut(s) 738, 1896
StyD4I CCNGG 2 cut(s) 185, 418
StyI CCWWGG 3 cut(s) 147, 327, 602
TaaI ACNGT 7 cut(s) 526, 1337, 1585, 1618, 1748, 1951, 2023
TaiI ACGT 5 cut(s) 145, 180, 429, 1630, 2138
TaqI TCGA 6 cut(s) 219, 586, 1433, 1701, 2132, 2143
TatI WGTACW 4 cut(s) 505, 970, 991, 2181
TfiI GAWTC 7 cut(s) 204, 280, 616, 1152, 1415, 1444, 1892
TscAI CASTG 4 cut(s) 1207, 1386, 2026, 2260
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 7 cut(s) 368, 647, 650, 1528, 1708, 1811, 1919
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 7 cut(s) 554, 719, 931, 1068, 1173, 1174, 1379
TspGWI ACGGA 3 cut(s) 188, 1186, 1463
TspRI CASTG 4 cut(s) 1207, 1386, 2026, 2260
Tth111I GACNNNGTC 3 cut(s) 67, 305, 1671
Van91I CCANNNNNTGG 1 cut(s) 1888
VpaK11BI GGWCC 4 cut(s) 1088, 1231, 1486, 2243
VspI ATTAAT 1 cut(s) 1829
XapI RAATTY 6 cut(s) 249, 926, 1736, 1774, 2009, 2168
XceI RCATGY 1 cut(s) 1277
XmnI GAANNNNTTC 4 cut(s) 765, 855, 1554, 1851
XspI CTAG 2 cut(s) 738, 1896
ZraI GACGTC 1 cut(s) 2136
Zsp2I ATGCAT 1 cut(s) 1242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.