AT3G28020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000526
Physical Location & Seq
Reverse (-)
10417906 .. 10419149
1244 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G28020.1

Sequence Viewer

Length: 741 bp
ATGGCGGAATCGACAAAGCAACACATCGATCTAATCCGGGAGACTAAGTTCTCAATTGGCAGAGCTTATAATCCTTTGACGGAGGATCTTCATCAGGCTCACCGCAATTGCAACCTTGGCTACATTGTTCCTGAGTGGGTTGAGAAACGTCCTTCTCTGGTCGATATTCAAAAGATTAATGGTTTTGGTTCTGCTATTCCGACCACAACAATAATCATGCCTTTGAAAAGTGACAAAGTAAAGCCAGTGAAAGAACAACTCTCCAATGTTCATCCTGAGATCCACTACCAAGATAAGGTCAGCATTGTTAACTCACACGGGATTGTTAGCGAGACTAATTTGGTGTCAAGGAAGAGCATTGATGCTGAGTCGCAAAAGTTAACGGTGAAAAATGAGAACCGTGTGGGAAGGAGAGGCGAAGTTGAGGAATTGTTGACAACGTTGGTGTTTCTACTCGGAGACCGTCTTGCCCTTGGAGATAGCTTTCCTCCTAGTATCTATGCGTTTCTTCCCACCGAGATGGAGATTTTATCCTTGCATCGTCTAGATGATATGTGGAACCAAGGGATTCTCAGCTGTGTTCCTTCTGCATTTGTGAATGCATTCACTTCTTTAGTGAAAAAGACAGATGCTTTTAGTTTCTTGCCTGTGAAGGTATCTAACTACGAAGAGTTGAATGATGTCCGAGAATCTATTATGGCAAGGGTTTTGGCAGAGGGAAATGTGCCTAGCAGACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

27.65

Weight (kDa)

6.18

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 69
AciI CCGC 2 cut(s) 5, 103
AclI AACGTT 1 cut(s) 440
AclWI GGATC 2 cut(s) 93, 274
AfiI CCNNNNNNNGG 1 cut(s) 295
AgsI TTSAA 3 cut(s) 170, 226, 676
AloI GAACNNNNNNTCC 2 cut(s) 32, 64
AluBI AGCT 3 cut(s) 65, 483, 576
AluI AGCT 3 cut(s) 65, 483, 576
Alw26I GTCTC 3 cut(s) 35, 326, 453
AlwI GGATC 2 cut(s) 93, 274
AseI ATTAAT 1 cut(s) 177
Asp700I GAANNNNTTC 1 cut(s) 602
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 2 cut(s) 92, 397
BccI CCATC 1 cut(s) 514
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 3 cut(s) 35, 326, 453
BfaI CTAG 3 cut(s) 492, 545, 729
Bme1390I CCNGG 1 cut(s) 38
BmiI GGNNCC 1 cut(s) 560
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 3 cut(s) 352, 547, 619
BpuMI CCSGG 1 cut(s) 38
Bsa29I ATCGAT 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 90
BsaI GGTCTC 1 cut(s) 453
BsaJI CCNNGG 3 cut(s) 115, 472, 562
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 1 cut(s) 295
Bse1I ACTGG 1 cut(s) 245
Bse8I GATNNNNATC 1 cut(s) 90
BseCI ATCGAT 1 cut(s) 27
BseDI CCNNGG 3 cut(s) 115, 472, 562
BseGI GGATG 1 cut(s) 271
BseJI GATNNNNATC 1 cut(s) 90
BseLI CCNNNNNNNGG 1 cut(s) 295
BseMII CTCAG 4 cut(s) 123, 267, 357, 586
BseNI ACTGG 1 cut(s) 245
BshVI ATCGAT 1 cut(s) 27
BsiSI CCGG 1 cut(s) 37
BslI CCNNNNNNNGG 1 cut(s) 295
BsmAI GTCTC 3 cut(s) 35, 326, 453
BsmI GAATGC 2 cut(s) 602, 604
Bso31I GGTCTC 1 cut(s) 453
Bsp143I GATC 3 cut(s) 28, 85, 279
BspACI CCGC 2 cut(s) 5, 103
BspCNI CTCAG 4 cut(s) 124, 268, 358, 585
BspDI ATCGAT 1 cut(s) 27
BspLI GGNNCC 1 cut(s) 560
BspPI GGATC 2 cut(s) 93, 274
BspQI GCTCTTC 1 cut(s) 347
BspTNI GGTCTC 1 cut(s) 453
BsrI ACTGG 1 cut(s) 245
BssECI CCNNGG 3 cut(s) 115, 472, 562
BssMI GATC 3 cut(s) 28, 85, 279
BssT1I CCWWGG 3 cut(s) 115, 472, 562
Bst4CI ACNGT 3 cut(s) 385, 401, 464
Bst6I CTCTTC 2 cut(s) 347, 663
BstDEI CTNAG 5 cut(s) 45, 132, 276, 366, 572
BstF5I GGATG 1 cut(s) 271
BstKTI GATC 3 cut(s) 31, 88, 282
BstMAI GTCTC 3 cut(s) 35, 326, 453
BstMBI GATC 3 cut(s) 28, 85, 279
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 36
BstX2I RGATCY 2 cut(s) 85, 279
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 2 cut(s) 85, 279
Bsu15I ATCGAT 1 cut(s) 27
BsuTUI ATCGAT 1 cut(s) 27
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 1 cut(s) 252
ClaI ATCGAT 1 cut(s) 27
CviAII CATG 1 cut(s) 217
CviJI RGCY 6 cut(s) 65, 98, 120, 244, 483, 576
CviKI_1 RGCY 6 cut(s) 65, 98, 120, 244, 483, 576
DdeI CTNAG 5 cut(s) 45, 132, 276, 366, 572
DpnI GATC 3 cut(s) 30, 87, 281
DpnII GATC 3 cut(s) 28, 85, 279
Eam1104I CTCTTC 2 cut(s) 347, 663
EarI CTCTTC 2 cut(s) 347, 663
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 115, 472, 562
Eco31I GGTCTC 1 cut(s) 453
EcoT14I CCWWGG 3 cut(s) 115, 472, 562
EcoT22I ATGCAT 1 cut(s) 604
ErhI CCWWGG 3 cut(s) 115, 472, 562
FaeI CATG 1 cut(s) 220
FaiI YATR 5 cut(s) 69, 218, 501, 554, 698
FatI CATG 1 cut(s) 216
FokI GGATG 1 cut(s) 258
FspBI CTAG 3 cut(s) 492, 545, 729
HapII CCGG 1 cut(s) 37
Hin1II CATG 1 cut(s) 220
HincII GTYRAC 3 cut(s) 310, 381, 435
HindII GTYRAC 3 cut(s) 310, 381, 435
HinfI GANTC 4 cut(s) 8, 368, 568, 689
HpaI GTTAAC 2 cut(s) 310, 381
HpaII CCGG 1 cut(s) 37
HphI GGTGA 2 cut(s) 92, 397
Hpy166II GTNNAC 3 cut(s) 310, 381, 435
Hpy188I TCNGA 3 cut(s) 201, 458, 686
Hpy188III TCNNGA 3 cut(s) 131, 275, 545
Hpy8I GTNNAC 3 cut(s) 310, 381, 435
HpyAV CCTTC 4 cut(s) 162, 402, 594, 646
HpyCH4III ACNGT 3 cut(s) 385, 401, 464
HpyCH4IV ACGT 2 cut(s) 148, 440
HpyCH4V TGCA 4 cut(s) 111, 538, 590, 602
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 5 cut(s) 45, 132, 276, 366, 572
HpySE526I ACGT 2 cut(s) 148, 440
Hsp92II CATG 1 cut(s) 220
KspAI GTTAAC 2 cut(s) 310, 381
Kzo9I GATC 3 cut(s) 28, 85, 279
LguI GCTCTTC 1 cut(s) 347
LpnPI CCDG 7 cut(s) 50, 80, 143, 144, 258, 288, 660
LweI GCATC 3 cut(s) 352, 547, 619
MaeI CTAG 3 cut(s) 492, 545, 729
MaeII ACGT 2 cut(s) 148, 440
MaeIII GTNAC 1 cut(s) 230
MalI GATC 3 cut(s) 30, 87, 281
MboI GATC 3 cut(s) 28, 85, 279
MboII GAAGA 4 cut(s) 80, 364, 500, 680
MfeI CAATTG 2 cut(s) 54, 106
MflI RGATCY 2 cut(s) 85, 279
MluCI AATT 4 cut(s) 54, 106, 337, 428
MlyI GAGTC 1 cut(s) 377
MmeI TCCRAC 1 cut(s) 224
MnlI CCTC 5 cut(s) 76, 407, 418, 498, 709
Mph1103I ATGCAT 1 cut(s) 604
MroXI GAANNNNTTC 1 cut(s) 602
MseI TTAA 3 cut(s) 177, 309, 380
MslI CAYNNNNRTG 1 cut(s) 518
MspA1I CMGCKG 1 cut(s) 576
MspI CCGG 1 cut(s) 37
MspR9I CCNGG 1 cut(s) 38
MunI CAATTG 2 cut(s) 54, 106
Mva1269I GAATGC 2 cut(s) 602, 604
MwoI GCNNNNNNNGC 1 cut(s) 117
NciI CCSGG 1 cut(s) 38
NdeII GATC 3 cut(s) 28, 85, 279
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 1 cut(s) 560
NmuCI GTSAC 1 cut(s) 230
NsiI ATGCAT 1 cut(s) 604
PciSI GCTCTTC 1 cut(s) 347
PctI GAATGC 2 cut(s) 602, 604
PdmI GAANNNNTTC 1 cut(s) 602
PfeI GAWTC 3 cut(s) 8, 568, 689
PfoI TCCNGGA 1 cut(s) 36
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
PshBI ATTAAT 1 cut(s) 177
PsiI TTATAA 1 cut(s) 69
Psp1406I AACGTT 1 cut(s) 440
PspN4I GGNNCC 1 cut(s) 560
PsuI RGATCY 2 cut(s) 85, 279
PvuII CAGCTG 1 cut(s) 576
RseI CAYNNNNRTG 1 cut(s) 518
SapI GCTCTTC 1 cut(s) 347
SaqAI TTAA 3 cut(s) 177, 309, 380
Sau3AI GATC 3 cut(s) 28, 85, 279
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 1 cut(s) 38
SetI ASST 8 cut(s) 67, 117, 151, 300, 443, 485, 578, 657
SfaNI GCATC 3 cut(s) 352, 547, 619
SmiMI CAYNNNNRTG 1 cut(s) 518
Sse9I AATT 4 cut(s) 54, 106, 337, 428
SsiI CCGC 2 cut(s) 5, 103
SspMI CTAG 3 cut(s) 492, 545, 729
StyD4I CCNGG 1 cut(s) 36
StyI CCWWGG 3 cut(s) 115, 472, 562
TaaI ACNGT 3 cut(s) 385, 401, 464
TaiI ACGT 2 cut(s) 151, 443
TaqI TCGA 3 cut(s) 11, 27, 162
TasI AATT 4 cut(s) 54, 106, 337, 428
TfiI GAWTC 3 cut(s) 8, 568, 689
Tru1I TTAA 3 cut(s) 177, 309, 380
Tru9I TTAA 3 cut(s) 177, 309, 380
TscAI CASTG 1 cut(s) 252
TseFI GTSAC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 2 cut(s) 80, 260
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 1 cut(s) 252
VspI ATTAAT 1 cut(s) 177
XbaI TCTAGA 1 cut(s) 544
XmnI GAANNNNTTC 1 cut(s) 602
XspI CTAG 3 cut(s) 492, 545, 729
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.