Rh7CG105600

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000526
Physical Location & Seq
Forward (+)
8016346 .. 8022201
5856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG105600.1

Sequence Viewer

Length: 5106 bp
ATGACTACGCCGAAAGAGCACATAGAAGAGATAAGGAGGAAAAAGTTTTCCATAGGAGGGGAACTCAACCCTCTAACTGAGGATCTTCATCAGGCTGTTAAGAATCTCTCAGCAGAGCTCTATGCTAAAGACGTGCATTTCCTGATGGAACTCATTCAGAACGCTGAGGACAATGAGTATTCGGATGGAGTAGATCCATCACTTGAGTTTGTCATAACCTCCCAGGACATAACGGGCACGGGGGCTCCTGCTACATTGGTGGTTTTCAATAATGAGAAGGGTTTCTCTGCAAAAAATGTAGACTCCATTTGCAGTGTTGGAAGGTCGACAAAGAAAGGCAACAGGAAGCGTGGTTATATTGGGGAGAAAGGAATTGGGTTCAAAAGTGTGTTTCTGATCACGGCTCAGCCTTACATCTTCAGTAATGGCTATCAGATCAGATTCAGTGAAGAGCCTTGTGTGCATTGCAATGTCGGATACATAGTTCCTGAATGGGTCAATCAGAACCCAACTCTATCTGACATCAAACAGATTTATGGTTCCAATGCTGCCCTTCCTACCACTACACTGATTTTACCTCTAAAGGCCGAAAAGGTCAAGCCTGTGAAACAGCAGCTCTCAAGCATTCATCCTGAAGTTCTCTTATTTCTGTCAAAGGTTAAGCGGCTCTCTGTCAGGAAAGACAATGAGGATCCAAGGAAGAATACAGTAACTGCAATTGAGATAGAAAGCCAAACTGATTTTGTGACCAGGAAGAATATTGATGCTCAATCCTACACACTCCGTCTTTCTGCAGAAGAAAATGATGACAATGATTCCGAAAGGGAATGCAGTTACTACATGTGGAAACAAAAGTTTCCTGTCAGGCAGGAATTCAGAGTTGAGAGGAGAATGGAGGTGGATGAGTGGGTGATCACTCTTGCATTTCCAAATGGAGAACGTCTTCGAAGAGCAAGGAACTCTTCGCCTGGTGTTTATGCATTTCTTCCCACAGAGATGGTCACCAACTTTCCATTCATAATACAGGCGGATTTTCTTTTAGCATCTTCAAGAGAGACAATCCTACTTGACAACAAGTGGAATCAAGGGATTCTTGACTGTGTGCCCACCGCATTTGCCCATGCCTTTAACTCTCTTGTGAAAACTGTGGAAGGTGCGCCAACATCAAGTTTGGCTCCTATGTTCAGATTCTTGCCTGTTCAGGGTTCTTCCTATAAAGAATTAAATGCTGTAAGGGAGTCAATAAAAGCAAAGATGGTTGACGAAGACATTATACCAGTTGAGCCTGAAATGGAGCAGAGGTTCTTCCACAAGCCTCGTGAAGTGGGAAGATTAATGCCTGCCTTTTGGACTATTTTGAGGAAAGCGAGGGTTCAAGGTGTAAGCTTGATTAAGCTTTCATCACATGGAAGATTTATTCTGTCTCATTCCTTTGACAGAAAGGAATATGATCTTATACTAAGTTTCCTTGGAGTTGAACCAGTGAATGATGACTGGTATGTTAAATGCATACAGGGTACTTCAAATCTTGTTGATGGAGTGTCAGAAGATGTGTGCTTAGAGCTTCTACTGTTTATTGCTGACAATTGGGGTTCTAAATTTGGTCACACCAACATGAAGAACATACCATTGATAAAATATGCAGACTATGGAAATGAATCTTTGTGCAGCATAAGCCAAATCCAGAAAGGTGAATGTAGAGTTTTCTTATCGAGCAAATGTTCTCGTGTTTCATGGCTGATTGATTGCAACCAGGAATTTAATTCTGTGACTCGTCTTCTCTTTATGCCAAAAGCCACTCAGGAAGCCATCTGGGAATGCTCTGGGAAGGATACATTGAAGAAATGGCTAGCTGATCAAGCTAAGGTTCGTTCTGTTGATGTCAATCAATATGCAGTTGATCTCCTTCCGAATTCTCTTAATGAGCGGAAGCTTGTTATTGCATATGTTCATTTCTTATATCACTCCTTGGATAATGATTTCATCTCTAGTCGGGAAGTTGACCATTTATGCGGAAGTATGCCACTAGTGGATAGCTATGGGAACATTAACAGGAGTAGGAAAGGGGTTGTTGTTCCTGCCAATGGAAGCAAATGGGCGGGACTGACTGATTCTAATTTGTGGAGAAAAGAAGGCTATGTTGAATTAGGAGAGCTTTATATGTATTCTGGCAAATTTGCTGGTAAGTTTACACCGAAGAAGAAGCTTCTTGAGTTCCTTATAAAATATGCTGCTGCTTTGGATGTCCCTCATATTTCTGCTCCAAGTGATGGTATCTCATCAGTATCTGCACCACTAACTAAGCAGAATGCCTTCCTGCTTTTGGATTGGATTCGTCAACTGAATTATAAAAGGGTTGGCATCCCTAAGAAGTTCTTGGCTTGCATAAAGGAAGGTAGTTGGCTGAAAGTTACTTTGAATGGCTCTCCTGGTTATAGGCCACCATCTCAGTCATTTCTCCTTAACCCATCATGGGGAAATACTTTGCAGAATGGATCCGTCTCTGTTGACATTCCATTGATTGATACGAGCTTTTATGGTGAGAGTATTAGTGAATACAAGGAGGAGCTAAAGACAATTGGGGTCATGTTTGAATATAGTGAAGCATGTGACTTCATTGGGAAGCATTTCATGTCTCTGGCAGCTTCAACCTCTTTAACTAGAGGAAATGTGCATTCCATTCTGCGTTTCATCAGGTTTTTAAGGGACAAACTTCTTCCTCCGGCCGATTTTATCTCTGCAATTAAAAAAGGGAACTGGCTGAAAACTTCCGTTGGCTACAGGTCTCCTGTAGGATCTGTTTTGTCAGATAAGGAGTGGAATGTTGCCTCCAAATTTAGCAACATTCCTTTCATTGATCAGGACTTTTACGGTGATGAAATCTGTTATTTCAGAACTGAACTTGGGCTGCTCGGTGTTGTAATCTCCTTCAACAGAAGTTACCAACTTATAATTGACAACCTGAAGCCACCATCTTGCATAACTTCTTGGACAGCTGAGGTTGTTACCTTAATGCTCAACTGTATGGCTCTATCCAAATCATCTGACAAACTTGTCAATGCACTGAAAGGAGTCAAGTGCCTGAAGACAAGTATTGGTTACATGTGTCCAGGGGAATGTCTCTTGTTTCATCAAGAATGGGGTTGCATTCTTCAGGTCGTCAATGGTCTTCCTGTGATTGATCATGAATTCTATGGGAGCAACATTTTCTCGTACAGAAATGAACTGAGAAAGATCGGTGTGGCGGTTGATTTTGAAGAGGCTGCAAAAGTATTTGCTCGATATTTCAGGCACTATGCATCATCAGCTTCCATCACCAAGGAAAATGTTGCATCATTGCTTCTGTGTTGCAGAGCACTGAAGGGGACTCCATATAAATTTCCTGCTGATCTTAGGAGCTACATTGGTGAGGTTAAGTGGTTGCGTAGTCGTTTTGGTGATTACAGATGTCCAAGGGAGTGCATTCTCTTTGGTCCCGAGTGGGAACCTATATCCCCAATAACTCTGCTTCCTTTCATTGATGATTCTGACAGTTACTATGGAAATAGCATACATGAGTACAGAAAAGAGCTGAAGAGCTTGGGGGTTGTCGTTGAATACAAGGAAGGAGTTAAGTTTGTGGCTTCTGGTCTTTATCTCCCTAGGGATGCCAGCCGTATTTTTCCTGTAAATGCACTTGCGTTGCTGGAATGCATACGAGTTTTCCAGGAGAAAGGTTATGCCTTTCTTGATAATTCTGATTTCATGAAGAAAGTTTCTCAACCATGGATAAAGACTCATGCTGGTTATAGGTCTCCCAAAGACTCTTTGTTGTTTGATTCCAAGTTTGGCTTATATTTGAAGCGGACTGATGGGCCTTTCATTGATGAAGAATTCTATGGTACTAAGATTGCATCTTACAAAAAAGAGCTGTCTGCATTGGGGGTTATTGTTGTAGCTGAAAAGGGGTGTCCCTTGATTGCCGGCCACCTTGATATTCATAATGAGACTGAAGCTTTTGTTCGATTGTATAGTTACTTGAGTGCGTTCCAGTGGGAGCCAGACACTGACGCTGCTCGAAGAATCTGGATTCCAAAAGGGGAGTGGGTTCGTCCTGATGATTGTGTTATATATGACAAGGATGGGCTATTTGGTTTACAATTGACTGTTTTGGAGAAACATTATGAACAGGAACTTCTGCTTTTCTTCTCCAGTGCTTTTAGAGTTAAATCTCATCCTTCAGTTGATGATTACTTGAAGGTTTGGAAGGTTTGGGAAAGTTCTGGAAGTGCATTGTCGCATGCTGATTGTTGCAAGTTTTGGAGTTATATCTCGCAGCACTGGACTTCAAAGACAGAAAAGACCGTTGCTGATGCCTTGGTGAAAGTACCTGTCAGTTCTGGATCTGAAGGAATCTTGCTGTGCAACAAGTCTGATGTTTTCATTGCTGATGACCTTCAGCTGAAGTATCTATTCGAACAGTCTTCTCCTGAGTCTATTTTTGTTTGGTACCCTCAGCCAAGTTTGCCTTCCATTCCAAGATCCAAGTTGCTTGACATTTATCGAAGAATTGGTGTTCGCACTATATCTGAATCTGTACAGAAGGAAGAGGCGTCACTTGCAGATGATGTTGAAATAGTCTCTTTGCCGAGGGAAAAACTGATTAAGAAAGCGCTGTTCAGGCTGATCCTTGGTTTTCTTGCTGCTCCTCCCATGGAAATGGAAGCTGAGAAGAGGCTTGAAGCTGTTCAATTCCTTGTGAATGTTACTGTGGTGGTAACAGCTGAACCAATCTCTGTAAGCTATAATCTACCTTTATCTTCTGGGAAAGTTTTGAATGTGAGAGGAAGCCGAAAAATACGGTTTGATAGAGAAAATTCAAAGATCTTCACTCAGAAAATGGACAAGTCAGGAGGGCAGAAGAGTATCATTGAGTTTGCTACTTTTTTCTCTGAAGCGATATCTGAGAGTGTGTTATGGGAGAGCACTGATCATATAGATTCACTCTCTGAGCTGATCAAGATGGCTGCTTTGCTAGACTTCAATGAGGAAGCAGTTGATTTCTTGATGAAGTCCAAGAATTTGCAAATCTTCATGGAGGATGAGGAGTTTCTTAGAACTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1701

Amino Acids

192.82

Weight (kDa)

6.68

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 27 - 143 1.4e-11 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2222, 2349, 2951
AasI GACNNNNNNGTC 1 cut(s) 3053
Acc65I GGTACC 1 cut(s) 4488
AccB1I GGYRCC 1 cut(s) 4488
AccB7I CCANNNNNTGG 1 cut(s) 2270
AccBSI CCGCTC 1 cut(s) 1927
AccI GTMKAC 2 cut(s) 300, 326
AciI CCGC 8 cut(s) 664, 1028, 1110, 1927, 2013, 2099, 3245, 3844
AcoI YGGCCR 2 cut(s) 2724, 3964
AcyI GRCGYC 1 cut(s) 4592
AfaI GTAC 7 cut(s) 1519, 3215, 3560, 3883, 4368, 4490, 4578
AfeI AGCGCT 1 cut(s) 4653
AfiI CCNNNNNNNGG 7 cut(s) 57, 1202, 2084, 2270, 2323, 2473, 2474
AflIII ACRYGT 2 cut(s) 840, 3102
AhlI ACTAGT 1 cut(s) 2026
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 7 cut(s) 222, 749, 967, 1752, 2428, 3109, 3705
AjuI GAANNNNNNNTTGG 4 cut(s) 998, 1030, 4492, 4524
AloI GAACNNNNNNTCC 4 cut(s) 468, 500, 1286, 1318
Alw21I GWGCWC 4 cut(s) 21, 120, 3358, 4967
Alw26I GTCTC 9 cut(s) 1049, 1428, 2506, 2640, 2790, 3125, 3798, 3980, 4624
AlwNI CAGNNNCTG 3 cut(s) 713, 2288, 4046
Ama87I CYCGRG 1 cut(s) 3476
Aor51HI AGCGCT 1 cut(s) 4653
AoxI GGCC 5 cut(s) 585, 2438, 2724, 3854, 3964
AseI ATTAAT 1 cut(s) 1334
Asp700I GAANNNNTTC 6 cut(s) 153, 281, 942, 2312, 2627, 4725
Asp718I GGTACC 1 cut(s) 4488
AspA2I CCTAGG 1 cut(s) 3641
AspLEI GCGC 2 cut(s) 1159, 4654
AspS9I GGNCC 2 cut(s) 3473, 3854
AsuHPI GGTGA 9 cut(s) 922, 994, 1703, 2552, 2885, 3307, 3419, 3449, 4372
AsuII TTCGAA 2 cut(s) 946, 4455
AsuNHI GCTAGC 1 cut(s) 1849
AvaI CYCGRG 1 cut(s) 3476
AvaII GGWCC 1 cut(s) 3473
AvrII CCTAGG 1 cut(s) 3641
BaeGI GKGCMC 2 cut(s) 239, 1107
BamHI GGATCC 2 cut(s) 691, 2495
BanI GGYRCC 1 cut(s) 4488
BanII GRGCYC 2 cut(s) 120, 247
BarI GAAGNNNNNNTAC 2 cut(s) 1257, 1289
BauI CACGAG 2 cut(s) 1317, 1725
BbsI GAAGAC 6 cut(s) 935, 1272, 1769, 3092, 3161, 4455
Bbv12I GWGCWC 4 cut(s) 21, 120, 3358, 4967
BbvCI CCTCAGC 3 cut(s) 165, 2997, 4494
BceAI ACGGC 2 cut(s) 417, 3639
BciT130I CCWGG 7 cut(s) 224, 751, 969, 1754, 2430, 3111, 3707
BciVI GTATCC 2 cut(s) 470, 1825
BclI TGATCA 7 cut(s) 396, 912, 1855, 2857, 3181, 4969, 4995
BcoDI GTCTC 9 cut(s) 1049, 1428, 2506, 2640, 2790, 3125, 3798, 3980, 4624
BcuI ACTAGT 1 cut(s) 2026
BfaI CTAG 7 cut(s) 1850, 1989, 2027, 2661, 3642, 5015, 5104
BfmI CTRYAG 3 cut(s) 792, 2779, 2790
BfoI RGCGCY 1 cut(s) 4655
BfuI GTATCC 2 cut(s) 470, 1825
BglII AGATCT 1 cut(s) 4863
BlnI CCTAGG 1 cut(s) 3641
BlpI GCTNAGC 1 cut(s) 405
Bme1390I CCNGG 7 cut(s) 224, 751, 969, 1754, 2430, 3111, 3707
Bme18I GGWCC 1 cut(s) 3473
BmeT110I CYCGRG 1 cut(s) 3476
BmgBI CACGTC 1 cut(s) 133
BmgT120I GGNCC 2 cut(s) 3473, 3854
BmiI GGNNCC 9 cut(s) 246, 541, 693, 1176, 2497, 3475, 3486, 4038, 4490
BmrFI CCNGG 7 cut(s) 224, 751, 969, 1754, 2430, 3111, 3707
BmsI GCATC 8 cut(s) 754, 1052, 2370, 3308, 3341, 3637, 3902, 4342
BmtI GCTAGC 1 cut(s) 1853
BpiI GAAGAC 6 cut(s) 935, 1272, 1769, 3092, 3161, 4455
BplI GAGNNNNNCTC 2 cut(s) 48, 80
BpmI CTGGAG 1 cut(s) 4174
Bpu10I CCTNAGC 4 cut(s) 165, 1863, 2997, 4494
Bpu1102I GCTNAGC 1 cut(s) 405
Bpu14I TTCGAA 2 cut(s) 946, 4455
BpuEI CTTGAG 4 cut(s) 224, 604, 2231, 4039
BsaBI GATNNNNATC 2 cut(s) 87, 1884
BsaHI GRCGYC 1 cut(s) 4592
BsaI GGTCTC 2 cut(s) 2790, 3798
BsaXI ACNNNNNCTCC 4 cut(s) 229, 259, 1286, 1316
Bsc4I CCNNNNNNNGG 7 cut(s) 57, 1202, 2084, 2270, 2323, 2473, 2474
Bse118I RCCGGY 1 cut(s) 3962
Bse1I ACTGG 7 cut(s) 1277, 1481, 1499, 2762, 4030, 4191, 4325
Bse3DI GCAATG 4 cut(s) 463, 475, 3335, 4422
Bse8I GATNNNNATC 2 cut(s) 87, 1884
BseBI CCWGG 7 cut(s) 224, 751, 969, 1754, 2430, 3111, 3707
BseGI GGATG 9 cut(s) 190, 628, 907, 2248, 2361, 3652, 4126, 4213, 5086
BseJI GATNNNNATC 2 cut(s) 87, 1884
BseLI CCNNNNNNNGG 7 cut(s) 57, 1202, 2084, 2270, 2323, 2473, 2474
BseMI GCAATG 4 cut(s) 463, 475, 3335, 4422
BseNI ACTGG 7 cut(s) 1277, 1481, 1499, 2762, 4030, 4191, 4325
BseRI GAGGAG 4 cut(s) 901, 2579, 4677, 5099
BseSI GKGCMC 2 cut(s) 239, 1107
BseX3I CGGCCG 1 cut(s) 2724
BsgI GTGCAG 2 cut(s) 1687, 2274
Bsh1285I CGRYCG 1 cut(s) 2727
BshFI GGCC 5 cut(s) 587, 2440, 2726, 3856, 3966
BshNI GGYRCC 1 cut(s) 4488
BsiEI CGRYCG 1 cut(s) 2727
BsiHKAI GWGCWC 4 cut(s) 21, 120, 3358, 4967
BsiHKCI CYCGRG 1 cut(s) 3476
BsiSI CCGG 2 cut(s) 2723, 3963
BslFI GGGAC 6 cut(s) 2115, 2231, 2720, 3379, 3459, 3936
BslI CCNNNNNNNGG 7 cut(s) 57, 1202, 2084, 2270, 2323, 2473, 2474
BsmAI GTCTC 9 cut(s) 1049, 1428, 2506, 2640, 2790, 3125, 3798, 3980, 4624
BsmBI CGTCTC 1 cut(s) 2506
BsmFI GGGAC 6 cut(s) 2115, 2231, 2720, 3379, 3459, 3936
BsmI GAATGC 8 cut(s) 624, 833, 1823, 2314, 2674, 3147, 3462, 3695
BsnI GGCC 5 cut(s) 587, 2440, 2726, 3856, 3966
Bso31I GGTCTC 2 cut(s) 2790, 3798
BsoBI CYCGRG 1 cut(s) 3476
Bsp119I TTCGAA 2 cut(s) 946, 4455
Bsp1286I GDGCHC 7 cut(s) 21, 120, 239, 247, 1107, 3358, 4967
Bsp1407I TGTACA 1 cut(s) 4576
Bsp1720I GCTNAGC 1 cut(s) 405
Bsp19I CCATGG 2 cut(s) 3764, 4692
BspACI CCGC 8 cut(s) 664, 1028, 1110, 1927, 2013, 2099, 3245, 3844
BspANI GGCC 5 cut(s) 587, 2440, 2726, 3856, 3966
BspHI TCATGA 2 cut(s) 3184, 3744
BspLI GGNNCC 9 cut(s) 246, 541, 693, 1176, 2497, 3475, 3486, 4038, 4490
BspMAI CTGCAG 1 cut(s) 796
BspOI GCTAGC 1 cut(s) 1853
BspQI GCTCTTC 3 cut(s) 444, 943, 3569
BspT104I TTCGAA 2 cut(s) 946, 4455
BspT107I GGYRCC 1 cut(s) 4488
BspTNI GGTCTC 2 cut(s) 2790, 3798
BsrBI CCGCTC 1 cut(s) 1927
BsrDI GCAATG 4 cut(s) 463, 475, 3335, 4422
BsrFI RCCGGY 1 cut(s) 3962
BsrGI TGTACA 1 cut(s) 4576
BsrI ACTGG 7 cut(s) 1277, 1481, 1499, 2762, 4030, 4191, 4325
BssAI RCCGGY 1 cut(s) 3962
BssNI GRCGYC 1 cut(s) 4592
BssSI CACGAG 2 cut(s) 1317, 1725
Bst2BI CACGAG 2 cut(s) 1317, 1725
Bst2UI CCWGG 7 cut(s) 224, 751, 969, 1754, 2430, 3111, 3707
Bst6I CTCTTC 9 cut(s) 21, 444, 943, 967, 3252, 3569, 4581, 4706, 4895
BstACI GRCGYC 1 cut(s) 4592
BstAUI TGTACA 1 cut(s) 4576
BstBI TTCGAA 2 cut(s) 946, 4455
BstC8I GCNNGC 6 cut(s) 1341, 1851, 2383, 3652, 3964, 4281
BstDSI CCRYGG 2 cut(s) 3764, 4692
BstEII GGTNACC 1 cut(s) 1000
BstF5I GGATG 9 cut(s) 190, 628, 907, 2248, 2361, 3652, 4126, 4213, 5086
BstH2I RGCGCY 1 cut(s) 4655
BstHHI GCGC 2 cut(s) 1159, 4654
BstMAI GTCTC 9 cut(s) 1049, 1428, 2506, 2640, 2790, 3125, 3798, 3980, 4624
BstMCI CGRYCG 1 cut(s) 2727
BstMWI GCNNNNNNNGC 8 cut(s) 16, 460, 1674, 1859, 3305, 4504, 4598, 4660
BstNI CCWGG 7 cut(s) 224, 751, 969, 1754, 2430, 3111, 3707
BstNSI RCATGY 4 cut(s) 844, 2610, 3106, 4283
BstPI GGTNACC 1 cut(s) 1000
BstSCI CCNGG 7 cut(s) 222, 749, 967, 1752, 2428, 3109, 3705
BstSFI CTRYAG 3 cut(s) 792, 2779, 2790
BstSLI GKGCMC 2 cut(s) 239, 1107
BstV2I GAAGAC 6 cut(s) 935, 1272, 1769, 3092, 3161, 4455
BstX2I RGATCY 8 cut(s) 82, 193, 691, 2495, 2795, 4382, 4520, 4863
BstXI CCANNNNNNTGG 2 cut(s) 997, 4699
BstYI RGATCY 8 cut(s) 82, 193, 691, 2495, 2795, 4382, 4520, 4863
BstZI CGGCCG 1 cut(s) 2724
BsuI GTATCC 2 cut(s) 470, 1825
BsuRI GGCC 5 cut(s) 587, 2440, 2726, 3856, 3966
BtgI CCRYGG 2 cut(s) 3764, 4692
BtrI CACGTC 1 cut(s) 133
BtsCI GGATG 9 cut(s) 190, 628, 907, 2248, 2361, 3652, 4126, 4213, 5086
BtsI GCAGTG 1 cut(s) 319
Cac8I GCNNGC 6 cut(s) 1341, 1851, 2383, 3652, 3964, 4281
CaiI CAGNNNCTG 3 cut(s) 713, 2288, 4046
CciI TCATGA 2 cut(s) 3184, 3744
CfoI GCGC 2 cut(s) 1159, 4654
Cfr10I RCCGGY 1 cut(s) 3962
Cfr13I GGNCC 2 cut(s) 3473, 3854
CseI GACGC 2 cut(s) 4058, 4581
Csp6I GTAC 7 cut(s) 1518, 3214, 3559, 3882, 4367, 4489, 4577
CspCI CAANNNNNGTGG 2 cut(s) 1096, 1131
CviQI GTAC 7 cut(s) 1518, 3214, 3559, 3882, 4367, 4489, 4577
DrdI GACNNNNNNGTC 1 cut(s) 3053
DseDI GACNNNNNNGTC 1 cut(s) 3053
EaeI YGGCCR 2 cut(s) 2724, 3964
EagI CGGCCG 1 cut(s) 2724
Eam1104I CTCTTC 9 cut(s) 21, 444, 943, 967, 3252, 3569, 4581, 4706, 4895
EarI CTCTTC 9 cut(s) 21, 444, 943, 967, 3252, 3569, 4581, 4706, 4895
EciI GGCGGA 1 cut(s) 1043
Ecl136II GAGCTC 1 cut(s) 118
EclXI CGGCCG 1 cut(s) 2724
Eco24I GRGCYC 2 cut(s) 120, 247
Eco31I GGTCTC 2 cut(s) 2790, 3798
Eco32I GATATC 1 cut(s) 4941
Eco47I GGWCC 1 cut(s) 3473
Eco47III AGCGCT 1 cut(s) 4653
Eco52I CGGCCG 1 cut(s) 2724
Eco53kI GAGCTC 1 cut(s) 118
Eco88I CYCGRG 1 cut(s) 3476
Eco91I GGTNACC 1 cut(s) 1000
EcoICRI GAGCTC 1 cut(s) 118
EcoO65I GGTNACC 1 cut(s) 1000
EcoRI GAATTC 4 cut(s) 872, 1912, 3188, 3872
EcoRII CCWGG 7 cut(s) 222, 749, 967, 1752, 2428, 3109, 3705
EcoRV GATATC 1 cut(s) 4941
EcoT22I ATGCAT 4 cut(s) 982, 1511, 3301, 3695
EcoT38I GRGCYC 2 cut(s) 120, 247
Esp3I CGTCTC 1 cut(s) 2506
FaqI GGGAC 6 cut(s) 2115, 2231, 2720, 3379, 3459, 3936
FauI CCCGC 1 cut(s) 2092
FauNDI CATATG 1 cut(s) 1945
FbaI TGATCA 7 cut(s) 396, 912, 1855, 2857, 3181, 4969, 4995
FblI GTMKAC 2 cut(s) 300, 326
FokI GGATG 9 cut(s) 197, 615, 914, 2255, 2348, 3659, 4133, 4200, 5093
FriOI GRGCYC 2 cut(s) 120, 247
FspBI CTAG 7 cut(s) 1850, 1989, 2027, 2661, 3642, 5015, 5104
GlaI GCGC 2 cut(s) 1158, 4653
GsuI CTGGAG 1 cut(s) 4174
HaeII RGCGCY 1 cut(s) 4655
HaeIII GGCC 5 cut(s) 587, 2440, 2726, 3856, 3966
HapII CCGG 2 cut(s) 2723, 3963
HgaI GACGC 2 cut(s) 4058, 4581
HhaI GCGC 2 cut(s) 1159, 4654
Hin1I GRCGYC 1 cut(s) 4592
Hin6I GCGC 2 cut(s) 1157, 4652
HinP1I GCGC 2 cut(s) 1157, 4652
HincII GTYRAC 5 cut(s) 327, 1261, 2002, 2339, 2509
HindII GTYRAC 5 cut(s) 327, 1261, 2002, 2339, 2509
HindIII AAGCTT 5 cut(s) 1384, 1394, 1931, 2204, 3993
HpaII CCGG 2 cut(s) 2723, 3963
HphI GGTGA 9 cut(s) 922, 994, 1703, 2552, 2885, 3307, 3419, 3449, 4372
Hpy166II GTNNAC 8 cut(s) 301, 327, 1261, 2002, 2190, 2339, 2509, 4136
Hpy8I GTNNAC 8 cut(s) 301, 327, 1261, 2002, 2190, 2339, 2509, 4136
HpyCH4IV ACGT 2 cut(s) 132, 940
HpyF10VI GCNNNNNNNGC 8 cut(s) 16, 460, 1674, 1859, 3305, 4504, 4598, 4660
HpySE526I ACGT 2 cut(s) 132, 940
Hsp92I GRCGYC 1 cut(s) 4592
HspAI GCGC 2 cut(s) 1157, 4652
KpnI GGTACC 1 cut(s) 4492
KroI GCCGGC 1 cut(s) 3962
KroNI GCCGGC 1 cut(s) 3964
Ksp22I TGATCA 7 cut(s) 396, 912, 1855, 2857, 3181, 4969, 4995
LguI GCTCTTC 3 cut(s) 444, 943, 3569
LmnI GCTCC 9 cut(s) 250, 1180, 1294, 2266, 2566, 3198, 3396, 4036, 4690
LweI GCATC 8 cut(s) 754, 1052, 2370, 3308, 3341, 3637, 3902, 4342
MaeI CTAG 7 cut(s) 1850, 1989, 2027, 2661, 3642, 5015, 5104
MaeII ACGT 2 cut(s) 132, 940
MbiI CCGCTC 1 cut(s) 1927
MfeI CAATTG 4 cut(s) 717, 1585, 2577, 4139
MflI RGATCY 8 cut(s) 82, 193, 691, 2495, 2795, 4382, 4520, 4863
MhlI GDGCHC 7 cut(s) 21, 120, 239, 247, 1107, 3358, 4967
MlyI GAGTC 8 cut(s) 296, 1247, 1765, 3081, 3361, 3769, 3797, 4481
MmeI TCCRAC 2 cut(s) 298, 454
Mph1103I ATGCAT 4 cut(s) 982, 1511, 3301, 3695
MroNI GCCGGC 1 cut(s) 3962
MroXI GAANNNNTTC 6 cut(s) 153, 281, 942, 2312, 2627, 4725
MslI CAYNNNNRTG 4 cut(s) 468, 995, 1613, 4697
MspA1I CMGCKG 3 cut(s) 2996, 4441, 4763
MspI CCGG 2 cut(s) 2723, 3963
MspR9I CCNGG 7 cut(s) 224, 751, 969, 1754, 2430, 3111, 3707
MunI CAATTG 4 cut(s) 717, 1585, 2577, 4139
Mva1269I GAATGC 8 cut(s) 624, 833, 1823, 2314, 2674, 3147, 3462, 3695
MvaI CCWGG 7 cut(s) 224, 751, 969, 1754, 2430, 3111, 3707
MwoI GCNNNNNNNGC 8 cut(s) 16, 460, 1674, 1859, 3305, 4504, 4598, 4660
NaeI GCCGGC 1 cut(s) 3964
NcoI CCATGG 2 cut(s) 3764, 4692
NdeI CATATG 1 cut(s) 1945
NgoMIV GCCGGC 1 cut(s) 3962
NheI GCTAGC 1 cut(s) 1849
NlaIV GGNNCC 9 cut(s) 246, 541, 693, 1176, 2497, 3475, 3486, 4038, 4490
NmeAIII GCCGAG 1 cut(s) 4653
NmuCI GTSAC 6 cut(s) 745, 1000, 1604, 1768, 2609, 4593
NsiI ATGCAT 4 cut(s) 982, 1511, 3301, 3695
NspI RCATGY 4 cut(s) 844, 2610, 3106, 4283
NspV TTCGAA 2 cut(s) 946, 4455
PaeI GCATGC 1 cut(s) 4283
PagI TCATGA 2 cut(s) 3184, 3744
PciI ACATGT 2 cut(s) 840, 3102
PciSI GCTCTTC 3 cut(s) 444, 943, 3569
PctI GAATGC 8 cut(s) 624, 833, 1823, 2314, 2674, 3147, 3462, 3695
PdiI GCCGGC 1 cut(s) 3964
PdmI GAANNNNTTC 6 cut(s) 153, 281, 942, 2312, 2627, 4725
PflMI CCANNNNNTGG 1 cut(s) 2270
PfoI TCCNGGA 1 cut(s) 3705
PleI GAGTC 8 cut(s) 296, 1246, 1765, 3080, 3361, 3769, 3797, 4480
PpsI GAGTC 8 cut(s) 296, 1246, 1765, 3080, 3361, 3769, 3797, 4480
PscI ACATGT 2 cut(s) 840, 3102
PshBI ATTAAT 1 cut(s) 1334
PsiI TTATAA 3 cut(s) 2222, 2349, 2951
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 7 cut(s) 222, 749, 967, 1752, 2428, 3109, 3705
PspEI GGTNACC 1 cut(s) 1000
PspGI CCWGG 7 cut(s) 222, 749, 967, 1752, 2428, 3109, 3705
PspN4I GGNNCC 9 cut(s) 246, 541, 693, 1176, 2497, 3475, 3486, 4038, 4490
PspPI GGNCC 2 cut(s) 3473, 3854
PstI CTGCAG 1 cut(s) 796
PstNI CAGNNNCTG 3 cut(s) 713, 2288, 4046
PsuI RGATCY 8 cut(s) 82, 193, 691, 2495, 2795, 4382, 4520, 4863
PvuII CAGCTG 3 cut(s) 2996, 4441, 4763
RsaI GTAC 7 cut(s) 1519, 3215, 3560, 3883, 4368, 4490, 4578
RsaNI GTAC 7 cut(s) 1518, 3214, 3559, 3882, 4367, 4489, 4577
RseI CAYNNNNRTG 4 cut(s) 468, 995, 1613, 4697
SacI GAGCTC 1 cut(s) 120
SalI GTCGAC 1 cut(s) 325
SapI GCTCTTC 3 cut(s) 444, 943, 3569
Sau96I GGNCC 2 cut(s) 3473, 3854
SchI GAGTC 8 cut(s) 296, 1247, 1765, 3081, 3361, 3769, 3797, 4481
ScrFI CCNGG 7 cut(s) 224, 751, 969, 1754, 2430, 3111, 3707
SduI GDGCHC 7 cut(s) 21, 120, 239, 247, 1107, 3358, 4967
SfaNI GCATC 8 cut(s) 754, 1052, 2370, 3308, 3341, 3637, 3902, 4342
SfcI CTRYAG 3 cut(s) 792, 2779, 2790
SfuI TTCGAA 2 cut(s) 946, 4455
SinI GGWCC 1 cut(s) 3473
SmiMI CAYNNNNRTG 4 cut(s) 468, 995, 1613, 4697
SmlI CTYRAG 4 cut(s) 203, 619, 2210, 4018
SmoI CTYRAG 4 cut(s) 203, 619, 2210, 4018
SpeI ACTAGT 1 cut(s) 2026
SphI GCATGC 1 cut(s) 4283
SsiI CCGC 8 cut(s) 664, 1028, 1110, 1927, 2013, 2099, 3245, 3844
SspI AATATT 1 cut(s) 760
SspMI CTAG 7 cut(s) 1850, 1989, 2027, 2661, 3642, 5015, 5104
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 7 cut(s) 222, 749, 967, 1752, 2428, 3109, 3705
TaiI ACGT 2 cut(s) 135, 943
TaqI TCGA 8 cut(s) 326, 946, 1712, 3280, 4003, 4057, 4455, 4543
TatI WGTACW 2 cut(s) 3558, 4576
TauI GCSGC 1 cut(s) 667
TseFI GTSAC 6 cut(s) 745, 1000, 1604, 1768, 2609, 4593
Tsp45I GTSAC 6 cut(s) 745, 1000, 1604, 1768, 2609, 4593
TspGWI ACGGA 3 cut(s) 773, 2488, 2761
Van91I CCANNNNNTGG 1 cut(s) 2270
VpaK11BI GGWCC 1 cut(s) 3473
VspI ATTAAT 1 cut(s) 1334
XceI RCATGY 4 cut(s) 844, 2610, 3106, 4283
XcmI CCANNNNNNNNNTGG 1 cut(s) 4080
XmaJI CCTAGG 1 cut(s) 3641
XmiI GTMKAC 2 cut(s) 300, 326
XmnI GAANNNNTTC 6 cut(s) 153, 281, 942, 2312, 2627, 4725
XspI CTAG 7 cut(s) 1850, 1989, 2027, 2661, 3642, 5015, 5104
Zsp2I ATGCAT 4 cut(s) 982, 1511, 3301, 3695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.