Rw7G008750

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000526
Physical Location & Seq
Forward (+)
8060274 .. 8067550
7277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G008750.1

Sequence Viewer

Length: 4239 bp
ATGGCGACGACGATGACGCCAAGGGAGCACATAGAAGATATACGGAAGACCAAGTTCTCCATCGGAGAAAAGCTCAACCCTTTAACGGAGGATCTTCACCAGGCCGTCAAGAACCTCTCCGGCGAGCTTTACTCTAAAGATGTCCACTTTTTCATGGAACTCGTTCAGAATGCAGAAGACAATGAGTACTTGGAGGGGGTCGATCCATCCCTTGAGTTTGTGATTACATACCTCGATATAACGGCTACAGGGGCTCCAGCCACATTGCTGGTTTTCAATAATGAGAAGGGGTTTTCTCCCAAAAACATAGACTCCATTTGCAGTGTTGGACGGTCCACCAAGAAAGGCAACAGGAAGCGTGGTTATATTGGGGAGAAAGGAATTGGCTTCAAGAGTGTGTTTCTGATCACTGCTCAGCCTTACATATTTAGTAATGGTTATCAGATAAGGTTCAGCGAAGAGCCTTGTTCACATTGCAATGTTGGCTACATAGTACCTGAATGGGTTGAGCAGAATCCCACACTATCTGATATAAAAAAGATATATGGTTCTGCTACTGCCCTTCCTACCACAACACTGATCTTACCTCTGAAGCCCAACAAGGTTGAACCTGTGAAGAAACAACTCTCAATCATTCATCCAGAAGTTCTCTTGTTCCTATCAAAGATTAAGCGGCTCTCTGTTAGGGAAGACAAAGAGGATCCAAGGCAGAACACTGTGAGTGCAATTGAGATAGCAAGCAAGACTGAATTTGTGTCAAGGAAGAACATCGATGCTCAATCCTACACTCTGCATCTTTCAGCTGAAGAAAATGATGGAAATGGAGAATGCAGTTACTACATGTGGAAACAAAAGTTTCCTGTCAGACAGGAATTCAGAGTTGAGAGGAGAATGGAGGTGGATGAGTGGGTGATCACTCTTGCATTTCCAAATGGAGAACGTCTTCGAAGAGCAAGGAACTCTTCGCCTGGTGTTTATGCATTTCTTCCCACAGAGATGGTCACCAACTTTCCCTTCATAATACAGGCGGATTTTCTTTTAGCATCTTCAAGAGAGACAATCCTACTTGACAACAAGTGGAATCAAGGGATTCTTGAGTGTGTGCCCACCGCATTTTCCCATGCTTTTAACTCTCTTGTGAAAACTGTGGAAGGTGCGCCAACATCAAGTTTGCCTCCTATGTTCAGATTCTTGCCTGTTCAGGTTTCTTCCTATAAAGAATTAAATGCTGTAAGGGAGTCAATAAAAGCAAAGATGGTTGACGAAGACATTATACCAGTTGAGCCTGAAATGGAGCAGAGGTTCTTCCACAAGCCTCGTGAAGTGGGAAGATTAATGCCTGCCTTTTGGACTATTTTGAGGAAGGCGAGGATTCAAGGTGTAAGCTTGATTAAGCTTTCATCACATGGAAGATTTGTTCTGTCTTATTCCTTTGACAGCAAGGAATATGATCCTATACTAAGTTTCCTTGGAGTTGAACTAGTGAATGATGCCTGGTATGTTAAATGCATACAGGGTACTTCTGATCTTGTTGAAGGAGTGTCAGAAGATGTGTACTTAGAGCTTCTACAGTTTATCGCTGACAATTGGGCTTCTAAATTTGGTCACACCAACATGAAGAACATACCATTGATAAAATATGCAGACTGTAGAAATGAATCTTTATGCAGCATAAGCGAAATCCAGAAAGGTGAATGTAGAGTTTTCTTATCGAGCAAAAGTTCTCATGTTTCATGGCTGATTGATTGCAACCGGGAATTTAATTCTGTGACTCGTCTTCTCTTTATGCCAAAAGCCACTCAGGAAGCTATCTGGGCATGCTCTGGGAAGGATACATTGAAGAAATGGCTAGCTGATCAAGCTAAGGTTGGTTCTGTTGATGTCAATAAATATGCAGTTGATCTACTTCAGAATTCTCTTCATGAGCGGAAGCTTGTTATTGCATATGTTCATTTCTTATATCACTCGGCTGATAATAATTTTATCTCAAGTCGGGAAGTTGACCGTTTATGTGGAATTATGCCACTAGTGGATGGCTATGGGAACATCATCAGAAGTAGAAAAGGCGTTGTTGTTGTTCCTGCCAATGGAAGCAAATGGGCGGGATTGACTGATTCTAATTTGTGGAGAAAAGAAGGCCATGTTGAATTAGGGGAGCATTATATGTATTCTGGCTACTTTTCTGGTAAGTATACACCGGAGAAGAAGCTTCTTGAGTTCCTTATAAAACATGTTGCTGCTTTGGATGTCCCTCATATTTCCGCCCCAAGTGATGGTATCACGTCTGTATCTGCACCACTAACTAAGCAGAATGCCTTCTTGCTTTTGGATTGGATTCGTCAACTGAATTATAAAAGGGTTGGCATCCCTCAGAAGTTCTTGGCTTGCATAAACGAAGGTAGTTGGCTGAAAGTTACTTTGAATGGCTCTCCTGGTTATAGGCCGCCATCCCAGTCATTTCTGCTTAACTCATCATGGGGAAATACTTTGCAGAATGGATCCGTCTCTGTTGACATTCCATTGATTGATACGAGCTTTTATGGTGAGAGCATTAGTGGATACAAGGAGGAGCTAAAGACAATTGGGGTCATGTTTGAATATAGCGAAGCATGTGACTTCATTGGGAAGCATTTCATGTCTCTGGCAGCTTCATCCTCTTTAACTAGAGGAAATGTGCATTCCATTCTGCGTTTCATCAGGTTTTTAAGGGACAAACTTCTTTCTCCGGCCGATTTTATCTCTGCTATTAAGAAAGGGAACTGGCTGAAGACTTCCGTTGGCTACAGGTCTCCTGTAGGATCTGTTTTGTCAGATAAGGAGTGGAATGTTGCCTCCAAAATTAGCAACATTCCCTTCATTGATCAGGACTTTTACGGAGATGAAATCTGTAATTTCAGAACTGAACTTGGGCTGCTTGGTGTTGTAATCTCCTTCAACAGAAGTTACCAACTTATAATTGACAACCTGAAGCCACCATCTTGCATAACTTCTTGGACACCTGAGGTTGTTACCTTAATGCTCAACTGTATGGCTTTATCCCAATCATCTGACAAACTTGTCAATGCACTGAAAGGAGTCAAGTGCCTGAAGACAATTATTGGTTACAAGTGTCCAGGGGAATGTCTCTTGTTTCATCACGAATGGGGTTGCATTCTTCAGGTCGTCAATGGTCTTCCCGTGATTGATCATGAATTCTATGGGAGCAACATTTTCCGGTACATAAATGAACTGAAAAAAATTGGTGTGGTGGTTGATTTTGAAGGGGCTGCAAAAGTACTTGCTCGATATTTCAGGCACTATGCATCATCAGCTTCCATCACCAAGGAAAATGTTGCATCATTGCTTCTGTGTTACAGAGAGCTGAATGGGACTCCATATAAATTTCCTGCTGATCTTAGGAGCTGCTTCGCTAAGGTCAAGTGGTTGCGTAGTCGTTTTGGTGATTACAGATGTCCAAGGGAGTGCATTCTCTTTAGTCCCGAGTGGGAATCTATATCCCCAATAACTCTGCTTCCTTTCATTGATGATTCTGACAGTTACTATGGAAAGGGCATACATGAGTACAGAAAAGAGTTGAAGAGCTTGGGGGTTGTCGTCGAATACAAGGAAGGAGTTAAGTTTGTGGCTCGTGGTCTTTATCTCCCTAGGGATGCCAGCAGTATTTTTCCTGTAAATGCACTTGCGTTGCTGGAATGCATACGTGTTTTCCAGAAGAAAGGTTACGCCTTTCTTGATAATTCTGATTTCATGAAGAAAGTTTCTCAACCATGGATAAAGACTCATGCTGGTTATAGGTCTCCCAAAGAGTCTTTGTTGTTTGATTCCAAGTTTGGCTTATATTTGAAGCGGACTGATGGGCCTTTCATTGATGAAGAATTCTATGGTACTAAGATTGCATCTTACAAAAAAGAGCTGTCTGCATTGGGGGTTATTGTTGAAGCTGAAAAGGGATGTCCCTTGATTGCCGGCCACCTTGATATTCATGATGAGACTGAAGCTTTTGTTCGGTTGTATAATTACTTGAGTGCGTTCCAGTGGGAGCCAGACACTGACGCTGCGCGAAGGATCTGGATTCCAAAAGGGGAGTGGGTTCGTGCTGATGAATGTGTTATTTATGACAAGGATGGGCTATTTGGTTTGCAGTTGACTGTTTTGGAGAAACATTATGAGCATAAACTTCTGCTTTTTCTTCCAGTGCGTTTGGAGTTAAATCTCATCCTTCAGTTGATGATTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1412

Amino Acids

160.18

Weight (kDa)

7.92

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 30 - 146 8.4e-11 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2225, 2352, 2954
AasI GACNNNNNNGTC 1 cut(s) 3056
AccB7I CCANNNNNTGG 1 cut(s) 2273
AccBSI CCGCTC 1 cut(s) 1927
AccI GTMKAC 1 cut(s) 2192
AccII CGCG 1 cut(s) 4060
AciI CCGC 8 cut(s) 673, 1028, 1110, 1927, 2102, 2262, 2444, 3847
AclWI GGATC 9 cut(s) 99, 197, 695, 708, 1445, 2493, 2506, 2806, 4073
AcoI YGGCCR 2 cut(s) 2727, 3967
AcsI RAATTY 8 cut(s) 749, 872, 1598, 1757, 1912, 3191, 3380, 3875
AcuI CTGAAG 9 cut(s) 611, 825, 1892, 2786, 2987, 3107, 3140, 4014, 4205
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 8 cut(s) 188, 495, 1519, 1556, 3218, 3276, 3563, 3886
AfiI CCNNNNNNNGG 3 cut(s) 85, 2087, 2273
AflIII ACRYGT 3 cut(s) 840, 2230, 3700
AhlI ACTAGT 2 cut(s) 1480, 2026
AjiI CACGTC 1 cut(s) 2283
AjnI CCWGG 5 cut(s) 99, 967, 1493, 2431, 3112
AjuI GAANNNNNNNTTGG 2 cut(s) 998, 1030
AloI GAACNNNNNNTCC 4 cut(s) 1286, 1318, 1401, 1433
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 7 cut(s) 1049, 2509, 2643, 2793, 3128, 3801, 3983
AlwI GGATC 9 cut(s) 99, 197, 695, 708, 1445, 2493, 2506, 2806, 4073
AlwNI CAGNNNCTG 1 cut(s) 4049
Ama87I CYCGRG 1 cut(s) 3479
AoxI GGCC 6 cut(s) 102, 2137, 2441, 2727, 3857, 3967
ApeKI GCWGC 7 cut(s) 1668, 2237, 2645, 2911, 3266, 3402, 4055
ApoI RAATTY 8 cut(s) 749, 872, 1598, 1757, 1912, 3191, 3380, 3875
AseI ATTAAT 1 cut(s) 1334
Asp700I GAANNNNTTC 4 cut(s) 162, 942, 2315, 2630
AspA2I CCTAGG 1 cut(s) 3644
AspLEI GCGC 2 cut(s) 1159, 4060
AspS9I GGNCC 2 cut(s) 333, 3857
AsuC2I CCSGG 1 cut(s) 1754
AsuHPI GGTGA 7 cut(s) 89, 922, 994, 1703, 2555, 3310, 3452
AsuII TTCGAA 1 cut(s) 946
AsuNHI GCTAGC 1 cut(s) 1849
AvaI CYCGRG 1 cut(s) 3479
AvaII GGWCC 1 cut(s) 333
AvrII CCTAGG 1 cut(s) 3644
AxyI CCTNAGG 1 cut(s) 3000
BaeGI GKGCMC 1 cut(s) 1107
BaeI ACNNNNGTAYC 2 cut(s) 2271, 2304
BamHI GGATCC 2 cut(s) 700, 2498
BanII GRGCYC 1 cut(s) 256
BarI GAAGNNNNNNTAC 4 cut(s) 1257, 1289, 3704, 3736
BauI CACGAG 2 cut(s) 1317, 3627
BbsI GAAGAC 9 cut(s) 53, 183, 696, 935, 1272, 1769, 2774, 3095, 3164
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 7 cut(s) 1680, 2224, 2657, 2898, 3253, 3389, 4042
BceAI ACGGC 2 cut(s) 89, 258
BciT130I CCWGG 5 cut(s) 101, 969, 1495, 2433, 3114
BciVI GTATCC 2 cut(s) 1825, 2552
BclI TGATCA 5 cut(s) 405, 912, 1855, 2860, 3184
BcnI CCSGG 1 cut(s) 1754
BcoDI GTCTC 7 cut(s) 1049, 2509, 2643, 2793, 3128, 3801, 3983
BcuI ACTAGT 2 cut(s) 1480, 2026
BfaI CTAG 5 cut(s) 1481, 1850, 2027, 2664, 3645
BfmI CTRYAG 5 cut(s) 246, 1568, 1648, 2782, 2793
BfuI GTATCC 2 cut(s) 1825, 2552
BisI GCNGC 9 cut(s) 674, 1669, 2238, 2444, 2646, 2912, 3267, 3403, 4056
BlnI CCTAGG 1 cut(s) 3644
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 9 cut(s) 675, 1670, 2239, 2445, 2647, 2913, 3268, 3404, 4057
BmcAI AGTACT 2 cut(s) 188, 3276
Bme1390I CCNGG 6 cut(s) 101, 969, 1495, 1754, 2433, 3114
Bme18I GGWCC 1 cut(s) 333
BmeT110I CYCGRG 1 cut(s) 3479
BmgBI CACGTC 1 cut(s) 2283
BmgT120I GGNCC 2 cut(s) 333, 3857
BmiI GGNNCC 4 cut(s) 255, 702, 2500, 4041
BmrFI CCNGG 6 cut(s) 101, 969, 1495, 1754, 2433, 3114
BmrI ACTGGG 1 cut(s) 2446
BmsI GCATC 9 cut(s) 763, 802, 1052, 1480, 2373, 3311, 3344, 3640, 3905
BmtI GCTAGC 1 cut(s) 1853
BmuI ACTGGG 1 cut(s) 2446
BpiI GAAGAC 9 cut(s) 53, 183, 696, 935, 1272, 1769, 2774, 3095, 3164
BplI GAGNNNNNCTC 4 cut(s) 57, 89, 116, 148
BpmI CTGGAG 1 cut(s) 240
Bpu10I CCTNAGC 2 cut(s) 1863, 3411
Bpu1102I GCTNAGC 1 cut(s) 414
Bpu14I TTCGAA 1 cut(s) 946
BpuEI CTTGAG 5 cut(s) 233, 1115, 1972, 2234, 4042
BpuMI CCSGG 1 cut(s) 1754
Bsa29I ATCGAT 1 cut(s) 771
BsaAI YACGTR 1 cut(s) 3701
BsaHI GRCGYC 1 cut(s) 17
BsaI GGTCTC 2 cut(s) 2793, 3801
BsaJI CCNNGG 8 cut(s) 20, 704, 1468, 3113, 3321, 3455, 3644, 3767
BsaWI WCCGGW 2 cut(s) 2197, 3213
BsaXI ACNNNNNCTCC 6 cut(s) 238, 268, 296, 326, 1286, 1316
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 2087, 2273
Bse118I RCCGGY 1 cut(s) 3965
Bse1I ACTGG 5 cut(s) 1277, 2452, 2765, 4033, 4193
Bse21I CCTNAGG 1 cut(s) 3000
Bse3DI GCAATG 4 cut(s) 263, 472, 484, 3338
BseBI CCWGG 5 cut(s) 101, 969, 1495, 2433, 3114
BseCI ATCGAT 1 cut(s) 771
BseDI CCNNGG 8 cut(s) 20, 704, 1468, 3113, 3321, 3455, 3644, 3767
BseLI CCNNNNNNNGG 3 cut(s) 85, 2087, 2273
BseMI GCAATG 4 cut(s) 263, 472, 484, 3338
BseMII CTCAG 4 cut(s) 428, 1814, 2384, 2991
BseNI ACTGG 5 cut(s) 1277, 2452, 2765, 4033, 4193
BseRI GAGGAG 2 cut(s) 901, 2582
BseSI GKGCMC 1 cut(s) 1107
BseX3I CGGCCG 1 cut(s) 2727
BseXI GCAGC 7 cut(s) 1680, 2224, 2657, 2898, 3253, 3389, 4042
BsgI GTGCAG 1 cut(s) 2277
Bsh1236I CGCG 1 cut(s) 4060
Bsh1285I CGRYCG 1 cut(s) 2730
BshFI GGCC 6 cut(s) 104, 2139, 2443, 2729, 3859, 3969
BshVI ATCGAT 1 cut(s) 771
BsiEI CGRYCG 1 cut(s) 2730
BsiHKAI GWGCWC 1 cut(s) 30
BsiHKCI CYCGRG 1 cut(s) 3479
BsiSI CCGG 6 cut(s) 120, 1753, 2198, 2726, 3214, 3966
BslFI GGGAC 5 cut(s) 2234, 2723, 3382, 3462, 3939
BslI CCNNNNNNNGG 3 cut(s) 85, 2087, 2273
BsmAI GTCTC 7 cut(s) 1049, 2509, 2643, 2793, 3128, 3801, 3983
BsmBI CGTCTC 1 cut(s) 2509
BsmFI GGGAC 5 cut(s) 2234, 2723, 3382, 3462, 3939
BsmI GAATGC 7 cut(s) 175, 833, 2317, 2677, 3150, 3465, 3698
BsnI GGCC 6 cut(s) 104, 2139, 2443, 2729, 3859, 3969
Bso31I GGTCTC 2 cut(s) 2793, 3801
BsoBI CYCGRG 1 cut(s) 3479
Bsp119I TTCGAA 1 cut(s) 946
Bsp1286I GDGCHC 3 cut(s) 30, 256, 1107
Bsp1720I GCTNAGC 1 cut(s) 414
Bsp19I CCATGG 1 cut(s) 3767
BspACI CCGC 8 cut(s) 673, 1028, 1110, 1927, 2102, 2262, 2444, 3847
BspANI GGCC 6 cut(s) 104, 2139, 2443, 2729, 3859, 3969
BspCNI CTCAG 4 cut(s) 427, 1813, 2383, 2992
BspDI ATCGAT 1 cut(s) 771
BspFNI CGCG 1 cut(s) 4060
BspHI TCATGA 4 cut(s) 1921, 3187, 3747, 3982
BspLI GGNNCC 4 cut(s) 255, 702, 2500, 4041
BspOI GCTAGC 1 cut(s) 1853
BspPI GGATC 9 cut(s) 99, 197, 695, 708, 1445, 2493, 2506, 2806, 4073
BspQI GCTCTTC 3 cut(s) 453, 943, 3572
BspT104I TTCGAA 1 cut(s) 946
BspTNI GGTCTC 2 cut(s) 2793, 3801
BsrBI CCGCTC 1 cut(s) 1927
BsrDI GCAATG 4 cut(s) 263, 472, 484, 3338
BsrFI RCCGGY 1 cut(s) 3965
BsrI ACTGG 5 cut(s) 1277, 2452, 2765, 4033, 4193
BssAI RCCGGY 1 cut(s) 3965
BssECI CCNNGG 8 cut(s) 20, 704, 1468, 3113, 3321, 3455, 3644, 3767
BssNAI GTATAC 1 cut(s) 2193
BssNI GRCGYC 1 cut(s) 17
BssSI CACGAG 2 cut(s) 1317, 3627
BssT1I CCWWGG 7 cut(s) 20, 704, 1468, 3321, 3455, 3644, 3767
Bst1107I GTATAC 1 cut(s) 2193
Bst2BI CACGAG 2 cut(s) 1317, 3627
Bst2UI CCWGG 5 cut(s) 101, 969, 1495, 2433, 3114
Bst4CI ACNGT 9 cut(s) 333, 718, 1147, 1572, 1649, 2006, 3026, 3536, 4150
Bst6I CTCTTC 5 cut(s) 453, 943, 967, 1923, 3572
BstACI GRCGYC 1 cut(s) 17
BstBAI YACGTR 1 cut(s) 3701
BstBI TTCGAA 1 cut(s) 946
BstC8I GCNNGC 8 cut(s) 125, 739, 1341, 1819, 1851, 2386, 3655, 3967
BstDSI CCRYGG 1 cut(s) 3767
BstEII GGTNACC 1 cut(s) 1000
BstFNI CGCG 1 cut(s) 4060
BstHHI GCGC 2 cut(s) 1159, 4060
BstMAI GTCTC 7 cut(s) 1049, 2509, 2643, 2793, 3128, 3801, 3983
BstMCI CGRYCG 1 cut(s) 2730
BstMWI GCNNNNNNNGC 7 cut(s) 25, 251, 483, 1674, 1814, 1859, 3308
BstNI CCWGG 5 cut(s) 101, 969, 1495, 2433, 3114
BstNSI RCATGY 4 cut(s) 844, 1821, 2234, 2613
BstPI GGTNACC 1 cut(s) 1000
BstSCI CCNGG 6 cut(s) 99, 967, 1493, 1752, 2431, 3112
BstSFI CTRYAG 5 cut(s) 246, 1568, 1648, 2782, 2793
BstSLI GKGCMC 1 cut(s) 1107
BstUI CGCG 1 cut(s) 4060
BstV1I GCAGC 7 cut(s) 1680, 2224, 2657, 2898, 3253, 3389, 4042
BstV2I GAAGAC 9 cut(s) 53, 183, 696, 935, 1272, 1769, 2774, 3095, 3164
BstX2I RGATCY 5 cut(s) 91, 700, 2498, 2798, 4065
BstXI CCANNNNNNTGG 2 cut(s) 268, 997
BstYI RGATCY 5 cut(s) 91, 700, 2498, 2798, 4065
BstZ17I GTATAC 1 cut(s) 2193
BstZI CGGCCG 1 cut(s) 2727
Bsu15I ATCGAT 1 cut(s) 771
Bsu36I CCTNAGG 1 cut(s) 3000
BsuI GTATCC 2 cut(s) 1825, 2552
BsuRI GGCC 6 cut(s) 104, 2139, 2443, 2729, 3859, 3969
BsuTUI ATCGAT 1 cut(s) 771
BtgI CCRYGG 1 cut(s) 3767
BtrI CACGTC 1 cut(s) 2283
BtsI GCAGTG 2 cut(s) 328, 408
BtsIMutI CAGTG 8 cut(s) 328, 408, 575, 714, 3065, 4040, 4047, 4200
Cac8I GCNNGC 8 cut(s) 125, 739, 1341, 1819, 1851, 2386, 3655, 3967
CaiI CAGNNNCTG 1 cut(s) 4049
CciI TCATGA 4 cut(s) 1921, 3187, 3747, 3982
CfoI GCGC 2 cut(s) 1159, 4060
Cfr10I RCCGGY 1 cut(s) 3965
Cfr13I GGNCC 2 cut(s) 333, 3857
ClaI ATCGAT 1 cut(s) 771
CseI GACGC 2 cut(s) 25, 4061
Csp6I GTAC 8 cut(s) 187, 494, 1518, 1555, 3217, 3275, 3562, 3885
CviQI GTAC 8 cut(s) 187, 494, 1518, 1555, 3217, 3275, 3562, 3885
DrdI GACNNNNNNGTC 1 cut(s) 3056
DseDI GACNNNNNNGTC 1 cut(s) 3056
EaeI YGGCCR 2 cut(s) 2727, 3967
EagI CGGCCG 1 cut(s) 2727
Eam1104I CTCTTC 5 cut(s) 453, 943, 967, 1923, 3572
EarI CTCTTC 5 cut(s) 453, 943, 967, 1923, 3572
EciI GGCGGA 2 cut(s) 1043, 2251
EclXI CGGCCG 1 cut(s) 2727
Eco130I CCWWGG 7 cut(s) 20, 704, 1468, 3321, 3455, 3644, 3767
Eco24I GRGCYC 1 cut(s) 256
Eco31I GGTCTC 2 cut(s) 2793, 3801
Eco47I GGWCC 1 cut(s) 333
Eco52I CGGCCG 1 cut(s) 2727
Eco57I CTGAAG 9 cut(s) 611, 825, 1892, 2786, 2987, 3107, 3140, 4014, 4205
Eco81I CCTNAGG 1 cut(s) 3000
Eco88I CYCGRG 1 cut(s) 3479
Eco91I GGTNACC 1 cut(s) 1000
EcoO65I GGTNACC 1 cut(s) 1000
EcoRI GAATTC 4 cut(s) 872, 1912, 3191, 3875
EcoRII CCWGG 5 cut(s) 99, 967, 1493, 2431, 3112
EcoT14I CCWWGG 7 cut(s) 20, 704, 1468, 3321, 3455, 3644, 3767
EcoT22I ATGCAT 4 cut(s) 982, 1511, 3304, 3698
EcoT38I GRGCYC 1 cut(s) 256
ErhI CCWWGG 7 cut(s) 20, 704, 1468, 3321, 3455, 3644, 3767
Esp3I CGTCTC 1 cut(s) 2509
FalI AAGNNNNNCTT 6 cut(s) 946, 978, 1077, 1109, 3818, 3850
FaqI GGGAC 5 cut(s) 2234, 2723, 3382, 3462, 3939
FauI CCCGC 1 cut(s) 2095
FauNDI CATATG 1 cut(s) 1945
FbaI TGATCA 5 cut(s) 405, 912, 1855, 2860, 3184
FblI GTMKAC 1 cut(s) 2192
Fnu4HI GCNGC 9 cut(s) 674, 1669, 2238, 2444, 2646, 2912, 3267, 3403, 4056
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 9 cut(s) 674, 1669, 2238, 2444, 2646, 2912, 3267, 3403, 4056
FspBI CTAG 5 cut(s) 1481, 1850, 2027, 2664, 3645
GlaI GCGC 2 cut(s) 1158, 4059
GluI GCNGC 9 cut(s) 674, 1669, 2238, 2444, 2646, 2912, 3267, 3403, 4056
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 6 cut(s) 104, 2139, 2443, 2729, 3859, 3969
HapII CCGG 6 cut(s) 120, 1753, 2198, 2726, 3214, 3966
HgaI GACGC 2 cut(s) 25, 4061
HhaI GCGC 2 cut(s) 1159, 4060
Hin1I GRCGYC 1 cut(s) 17
Hin6I GCGC 2 cut(s) 1157, 4058
HinP1I GCGC 2 cut(s) 1157, 4058
HincII GTYRAC 5 cut(s) 1261, 2002, 2342, 2512, 4146
HindII GTYRAC 5 cut(s) 1261, 2002, 2342, 2512, 4146
HindIII AAGCTT 5 cut(s) 1384, 1394, 1931, 2207, 3996
HpaII CCGG 6 cut(s) 120, 1753, 2198, 2726, 3214, 3966
HphI GGTGA 7 cut(s) 89, 922, 994, 1703, 2555, 3310, 3452
Hpy99I CGWCG 3 cut(s) 10, 13, 3599
HpyCH4III ACNGT 9 cut(s) 333, 718, 1147, 1572, 1649, 2006, 3026, 3536, 4150
HpyCH4IV ACGT 3 cut(s) 940, 2282, 3700
HpyF10VI GCNNNNNNNGC 7 cut(s) 25, 251, 483, 1674, 1814, 1859, 3308
HpySE526I ACGT 3 cut(s) 940, 2282, 3700
Hsp92I GRCGYC 1 cut(s) 17
HspAI GCGC 2 cut(s) 1157, 4058
KroI GCCGGC 1 cut(s) 3965
KroNI GCCGGC 1 cut(s) 3967
Ksp22I TGATCA 5 cut(s) 405, 912, 1855, 2860, 3184
LguI GCTCTTC 3 cut(s) 453, 943, 3572
LmnI GCTCC 8 cut(s) 25, 259, 1294, 2155, 2569, 3201, 3399, 4039
Lsp1109I GCAGC 7 cut(s) 1680, 2224, 2657, 2898, 3253, 3389, 4042
LweI GCATC 9 cut(s) 763, 802, 1052, 1480, 2373, 3311, 3344, 3640, 3905
MaeI CTAG 5 cut(s) 1481, 1850, 2027, 2664, 3645
MaeII ACGT 3 cut(s) 940, 2282, 3700
MbiI CCGCTC 1 cut(s) 1927
MfeI CAATTG 3 cut(s) 726, 1585, 2580
MflI RGATCY 5 cut(s) 91, 700, 2498, 2798, 4065
MhlI GDGCHC 3 cut(s) 30, 256, 1107
MlyI GAGTC 7 cut(s) 305, 1247, 1765, 3084, 3364, 3772, 3815
MmeI TCCRAC 1 cut(s) 307
Mph1103I ATGCAT 4 cut(s) 982, 1511, 3304, 3698
MroNI GCCGGC 1 cut(s) 3965
MroXI GAANNNNTTC 4 cut(s) 162, 942, 2315, 2630
MslI CAYNNNNRTG 3 cut(s) 477, 995, 1613
MspA1I CMGCKG 1 cut(s) 803
MspI CCGG 6 cut(s) 120, 1753, 2198, 2726, 3214, 3966
MspR9I CCNGG 6 cut(s) 101, 969, 1495, 1754, 2433, 3114
MunI CAATTG 3 cut(s) 726, 1585, 2580
Mva1269I GAATGC 7 cut(s) 175, 833, 2317, 2677, 3150, 3465, 3698
MvaI CCWGG 5 cut(s) 101, 969, 1495, 2433, 3114
MvnI CGCG 1 cut(s) 4060
MwoI GCNNNNNNNGC 7 cut(s) 25, 251, 483, 1674, 1814, 1859, 3308
NaeI GCCGGC 1 cut(s) 3967
NciI CCSGG 1 cut(s) 1754
NcoI CCATGG 1 cut(s) 3767
NdeI CATATG 1 cut(s) 1945
NgoMIV GCCGGC 1 cut(s) 3965
NheI GCTAGC 1 cut(s) 1849
NlaIV GGNNCC 4 cut(s) 255, 702, 2500, 4041
NmeAIII GCCGAG 1 cut(s) 1946
NmuCI GTSAC 4 cut(s) 1000, 1604, 1768, 2612
NsiI ATGCAT 4 cut(s) 982, 1511, 3304, 3698
NspI RCATGY 4 cut(s) 844, 1821, 2234, 2613
NspV TTCGAA 1 cut(s) 946
PaeI GCATGC 1 cut(s) 1821
PagI TCATGA 4 cut(s) 1921, 3187, 3747, 3982
PciI ACATGT 2 cut(s) 840, 2230
PciSI GCTCTTC 3 cut(s) 453, 943, 3572
PctI GAATGC 7 cut(s) 175, 833, 2317, 2677, 3150, 3465, 3698
PdiI GCCGGC 1 cut(s) 3967
PdmI GAANNNNTTC 4 cut(s) 162, 942, 2315, 2630
PflMI CCANNNNNTGG 1 cut(s) 2273
PkrI GCNGC 9 cut(s) 675, 1670, 2239, 2445, 2647, 2913, 3268, 3404, 4057
PleI GAGTC 7 cut(s) 305, 1246, 1765, 3083, 3364, 3772, 3814
PpsI GAGTC 7 cut(s) 305, 1246, 1765, 3083, 3364, 3772, 3814
Ppu21I YACGTR 1 cut(s) 3701
PscI ACATGT 2 cut(s) 840, 2230
PshBI ATTAAT 1 cut(s) 1334
PsiI TTATAA 3 cut(s) 2225, 2352, 2954
Psp6I CCWGG 5 cut(s) 99, 967, 1493, 2431, 3112
PspEI GGTNACC 1 cut(s) 1000
PspGI CCWGG 5 cut(s) 99, 967, 1493, 2431, 3112
PspN4I GGNNCC 4 cut(s) 255, 702, 2500, 4041
PspPI GGNCC 2 cut(s) 333, 3857
PstNI CAGNNNCTG 1 cut(s) 4049
PsuI RGATCY 5 cut(s) 91, 700, 2498, 2798, 4065
PvuII CAGCTG 1 cut(s) 803
RsaI GTAC 8 cut(s) 188, 495, 1519, 1556, 3218, 3276, 3563, 3886
RsaNI GTAC 8 cut(s) 187, 494, 1518, 1555, 3217, 3275, 3562, 3885
RseI CAYNNNNRTG 3 cut(s) 477, 995, 1613
SapI GCTCTTC 3 cut(s) 453, 943, 3572
SatI GCNGC 9 cut(s) 674, 1669, 2238, 2444, 2646, 2912, 3267, 3403, 4056
Sau96I GGNCC 2 cut(s) 333, 3857
ScaI AGTACT 2 cut(s) 188, 3276
SchI GAGTC 7 cut(s) 305, 1247, 1765, 3084, 3364, 3772, 3815
ScrFI CCNGG 6 cut(s) 101, 969, 1495, 1754, 2433, 3114
SduI GDGCHC 3 cut(s) 30, 256, 1107
SfaNI GCATC 9 cut(s) 763, 802, 1052, 1480, 2373, 3311, 3344, 3640, 3905
SfcI CTRYAG 5 cut(s) 246, 1568, 1648, 2782, 2793
SfuI TTCGAA 1 cut(s) 946
SinI GGWCC 1 cut(s) 333
SmiMI CAYNNNNRTG 3 cut(s) 477, 995, 1613
SmlI CTYRAG 5 cut(s) 212, 1094, 1987, 2213, 4021
SmoI CTYRAG 5 cut(s) 212, 1094, 1987, 2213, 4021
SpeI ACTAGT 2 cut(s) 1480, 2026
SphI GCATGC 1 cut(s) 1821
SsiI CCGC 8 cut(s) 673, 1028, 1110, 1927, 2102, 2262, 2444, 3847
SspMI CTAG 5 cut(s) 1481, 1850, 2027, 2664, 3645
StyD4I CCNGG 6 cut(s) 99, 967, 1493, 1752, 2431, 3112
StyI CCWWGG 7 cut(s) 20, 704, 1468, 3321, 3455, 3644, 3767
TaaI ACNGT 9 cut(s) 333, 718, 1147, 1572, 1649, 2006, 3026, 3536, 4150
TaiI ACGT 3 cut(s) 943, 2285, 3703
TaqI TCGA 7 cut(s) 201, 234, 771, 946, 1712, 3283, 3597
TatI WGTACW 4 cut(s) 186, 1554, 3274, 3561
TauI GCSGC 2 cut(s) 676, 2446
TscAI CASTG 8 cut(s) 328, 415, 582, 721, 3072, 4040, 4054, 4200
TseFI GTSAC 4 cut(s) 1000, 1604, 1768, 2612
TseI GCWGC 7 cut(s) 1668, 2237, 2645, 2911, 3266, 3402, 4055
Tsp45I GTSAC 4 cut(s) 1000, 1604, 1768, 2612
TspGWI ACGGA 5 cut(s) 58, 101, 2491, 2764, 2889
TspRI CASTG 8 cut(s) 328, 415, 582, 721, 3072, 4040, 4054, 4200
Van91I CCANNNNNTGG 1 cut(s) 2273
VpaK11BI GGWCC 1 cut(s) 333
VspI ATTAAT 1 cut(s) 1334
XapI RAATTY 8 cut(s) 749, 872, 1598, 1757, 1912, 3191, 3380, 3875
XceI RCATGY 4 cut(s) 844, 1821, 2234, 2613
XcmI CCANNNNNNNNNTGG 1 cut(s) 4083
XmaJI CCTAGG 1 cut(s) 3644
XmiI GTMKAC 1 cut(s) 2192
XmnI GAANNNNTTC 4 cut(s) 162, 942, 2315, 2630
XspI CTAG 5 cut(s) 1481, 1850, 2027, 2664, 3645
ZrmI AGTACT 2 cut(s) 188, 3276
Zsp2I ATGCAT 4 cut(s) 982, 1511, 3304, 3698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.