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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 114 |
| AciI | CCGC | 1 cut(s) | 195 |
| AcsI | RAATTY | 1 cut(s) | 89 |
| AcuI | CTGAAG | 1 cut(s) | 69 |
| AcyI | GRCGYC | 1 cut(s) | 111 |
| AgsI | TTSAA | 2 cut(s) | 151, 245 |
| AjnI | CCWGG | 1 cut(s) | 170 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 170, 202 |
| AluBI | AGCT | 1 cut(s) | 134 |
| AluI | AGCT | 1 cut(s) | 134 |
| Alw21I | GWGCWC | 1 cut(s) | 130 |
| Alw26I | GTCTC | 3 cut(s) | 113, 220, 287 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 122 |
| ApoI | RAATTY | 1 cut(s) | 89 |
| BbsI | GAAGAC | 1 cut(s) | 114 |
| Bbv12I | GWGCWC | 1 cut(s) | 130 |
| BccI | CCATC | 1 cut(s) | 211 |
| BciT130I | CCWGG | 1 cut(s) | 172 |
| BcoDI | GTCTC | 3 cut(s) | 113, 220, 287 |
| BisI | GCNGC | 1 cut(s) | 196 |
| BlsI | GCNGC | 1 cut(s) | 197 |
| Bme1390I | CCNGG | 1 cut(s) | 172 |
| BmrFI | CCNGG | 1 cut(s) | 172 |
| BpiI | GAAGAC | 1 cut(s) | 114 |
| BpmI | CTGGAG | 1 cut(s) | 144 |
| BsaHI | GRCGYC | 1 cut(s) | 111 |
| BsaJI | CCNNGG | 2 cut(s) | 102, 171 |
| Bse1I | ACTGG | 1 cut(s) | 127 |
| BseBI | CCWGG | 1 cut(s) | 172 |
| BseDI | CCNNGG | 2 cut(s) | 102, 171 |
| BseNI | ACTGG | 1 cut(s) | 127 |
| BsiHKAI | GWGCWC | 1 cut(s) | 130 |
| BsmAI | GTCTC | 3 cut(s) | 113, 220, 287 |
| Bsp1286I | GDGCHC | 1 cut(s) | 130 |
| BspACI | CCGC | 1 cut(s) | 195 |
| BsrI | ACTGG | 1 cut(s) | 127 |
| BssECI | CCNNGG | 2 cut(s) | 102, 171 |
| BssNI | GRCGYC | 1 cut(s) | 111 |
| BssT1I | CCWWGG | 1 cut(s) | 102 |
| Bst2UI | CCWGG | 1 cut(s) | 172 |
| Bst4CI | ACNGT | 1 cut(s) | 298 |
| Bst6I | CTCTTC | 2 cut(s) | 45, 240 |
| BstACI | GRCGYC | 1 cut(s) | 111 |
| BstMAI | GTCTC | 3 cut(s) | 113, 220, 287 |
| BstNI | CCWGG | 1 cut(s) | 172 |
| BstSCI | CCNGG | 1 cut(s) | 170 |
| BstV2I | GAAGAC | 1 cut(s) | 114 |
| BtsIMutI | CAGTG | 1 cut(s) | 303 |
| CaiI | CAGNNNCTG | 1 cut(s) | 122 |
| CviAII | CATG | 2 cut(s) | 181, 278 |
| CviJI | RGCY | 2 cut(s) | 134, 165 |
| CviKI_1 | RGCY | 2 cut(s) | 134, 165 |
| Eam1104I | CTCTTC | 2 cut(s) | 45, 240 |
| EarI | CTCTTC | 2 cut(s) | 45, 240 |
| Eco130I | CCWWGG | 1 cut(s) | 102 |
| Eco57I | CTGAAG | 1 cut(s) | 69 |
| EcoRII | CCWGG | 1 cut(s) | 170 |
| EcoT14I | CCWWGG | 1 cut(s) | 102 |
| ErhI | CCWWGG | 1 cut(s) | 102 |
| FaeI | CATG | 2 cut(s) | 184, 281 |
| FaiI | YATR | 5 cut(s) | 182, 200, 279, 285, 291 |
| FatI | CATG | 2 cut(s) | 180, 277 |
| Fnu4HI | GCNGC | 1 cut(s) | 196 |
| Fsp4HI | GCNGC | 1 cut(s) | 196 |
| GluI | GCNGC | 1 cut(s) | 196 |
| GsuI | CTGGAG | 1 cut(s) | 144 |
| Hin1I | GRCGYC | 1 cut(s) | 111 |
| Hin1II | CATG | 2 cut(s) | 184, 281 |
| HincII | GTYRAC | 1 cut(s) | 99 |
| HindII | GTYRAC | 1 cut(s) | 99 |
| HinfI | GANTC | 2 cut(s) | 5, 185 |
| Hpy166II | GTNNAC | 2 cut(s) | 99, 275 |
| Hpy188I | TCNGA | 1 cut(s) | 10 |
| Hpy8I | GTNNAC | 2 cut(s) | 99, 275 |
| HpyAV | CCTTC | 1 cut(s) | 62 |
| HpyCH4III | ACNGT | 1 cut(s) | 298 |
| HpyCH4IV | ACGT | 1 cut(s) | 111 |
| HpySE526I | ACGT | 1 cut(s) | 111 |
| Hsp92I | GRCGYC | 1 cut(s) | 111 |
| Hsp92II | CATG | 2 cut(s) | 184, 281 |
| LmnI | GCTCC | 1 cut(s) | 125 |
| LpnPI | CCDG | 5 cut(s) | 93, 108, 144, 157, 184 |
| MaeII | ACGT | 1 cut(s) | 111 |
| MaeIII | GTNAC | 1 cut(s) | 112 |
| MboII | GAAGA | 5 cut(s) | 24, 62, 77, 119, 257 |
| MhlI | GDGCHC | 1 cut(s) | 130 |
| MluCI | AATT | 4 cut(s) | 27, 89, 240, 256 |
| MnlI | CCTC | 1 cut(s) | 228 |
| MseI | TTAA | 3 cut(s) | 26, 239, 304 |
| MslI | CAYNNNNRTG | 1 cut(s) | 282 |
| MspR9I | CCNGG | 1 cut(s) | 172 |
| MvaI | CCWGG | 1 cut(s) | 172 |
| NlaIII | CATG | 2 cut(s) | 184, 281 |
| NmuCI | GTSAC | 1 cut(s) | 112 |
| PfeI | GAWTC | 2 cut(s) | 5, 185 |
| PflFI | GACNNNGTC | 1 cut(s) | 298 |
| PkrI | GCNGC | 1 cut(s) | 197 |
| Psp6I | CCWGG | 1 cut(s) | 170 |
| PspGI | CCWGG | 1 cut(s) | 170 |
| PstNI | CAGNNNCTG | 1 cut(s) | 122 |
| PsyI | GACNNNGTC | 1 cut(s) | 298 |
| RseI | CAYNNNNRTG | 1 cut(s) | 282 |
| SaqAI | TTAA | 3 cut(s) | 26, 239, 304 |
| SatI | GCNGC | 1 cut(s) | 196 |
| ScrFI | CCNGG | 1 cut(s) | 172 |
| SduI | GDGCHC | 1 cut(s) | 130 |
| SetI | ASST | 4 cut(s) | 73, 114, 136, 239 |
| SgeI | CNNG | 8 cut(s) | 92, 115, 135, 143, 183, 184, 193, 290 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 282 |
| Sse9I | AATT | 4 cut(s) | 27, 89, 240, 256 |
| SsiI | CCGC | 1 cut(s) | 195 |
| StyD4I | CCNGG | 1 cut(s) | 170 |
| StyI | CCWWGG | 1 cut(s) | 102 |
| TaaI | ACNGT | 1 cut(s) | 298 |
| TaiI | ACGT | 1 cut(s) | 114 |
| TasI | AATT | 4 cut(s) | 27, 89, 240, 256 |
| TauI | GCSGC | 1 cut(s) | 198 |
| TfiI | GAWTC | 2 cut(s) | 5, 185 |
| Tru1I | TTAA | 3 cut(s) | 26, 239, 304 |
| Tru9I | TTAA | 3 cut(s) | 26, 239, 304 |
| TscAI | CASTG | 1 cut(s) | 303 |
| TseFI | GTSAC | 1 cut(s) | 112 |
| Tsp45I | GTSAC | 1 cut(s) | 112 |
| TspDTI | ATGAA | 4 cut(s) | 24, 72, 101, 171 |
| TspRI | CASTG | 1 cut(s) | 303 |
| Tth111I | GACNNNGTC | 1 cut(s) | 298 |
| XapI | RAATTY | 1 cut(s) | 89 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 178 |
| ZraI | GACGTC | 1 cut(s) | 112 |