MD06G1135400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000526
Physical Location & Seq
Forward (+)
28042412 .. 28050535
8124 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1135400.v1.1.491

Sequence Viewer

Length: 5115 bp
ATGGCAACGCCAAGAGAGCACATTGAAGAAATACGCAAGAAAAAGTTCTCCATCGGTGAAGAAAAACTCAACCCTTTAACTGAAGATCTTCATCAGGCGGTTAAAAATCTTTCTCTTGAACTCTATACCAAATCTGTCCACTTTGTCATGGAACTCATTCAGAATGCAGAAGACAATGAGTACGCAGACGGCGTTGATCCGTCACTTGAATTTGTCGTAACGTCCAGGGATATAACCGGCACAGGGGCTCCTGCCACATTGCTGATTTTCAACAATGAGAAGGGATTTTCACGTAAGAACATAGAATCCATTTGCAGCATTGGACGGTCCACCAAGAAAGGCAACCGGAAGCGCGGCTACATTGGAGAGAAAGGAATTGGCTTCAAGAGTGTGTTTCTGATCACAGATCGGCCTTACATCTTTAGTAATGGCTATCAGATAAGGTTCAGTAAAGAGCCTTGTCTGCATTGCAATCTCGGGTACATTGTTCCTGAATGGGTTGAGGAAAACCCTACTCTATGTGACATAAAACAGATATATGGTTATGGTTCTGCCCTTCCAACCACAACGCTGATCTTACCTCTAAAGCCTGACGAGGTCCATCCTGTGAAGCAGCAGCTCTCAAGAATGCATCCGGAAGTTCTCTTGTTTCTTTCAAAGATTAAGCGGCTCTCGGTTAGGGAACATAATGAGGATCCAAGGCTCAACACTGTGAGCGCGATATCAATATCAAGTGAGATTGATCTTGTGGCGCAGAAGAACATTGATGCTCAGTCCTACACACTCCACCTCTCTGCAGAAGAAAATGGTGATGACGACAAAAACTGTATCTATTATATGTGGAAGCAGAAGTTTCCTGTCAGGCAAGAGTTCAGAGAGGAGAGGAGAATGGAGGTCGATGAGTGGGTGATCACGCTAGCATTTCCATATGGGGAGCGCCTTCATAGAGGAACAAGCTCACCCGGGGTCTATGCATTCCTTCCAACTCAGATGTTTACAAATTTGCCTTTCATAATTCAGGCAGATTTTCTGTTACAATCATCAAGAGAAGGTATTGTCTTGGACAGCAAGTGGAATCGAGGAATTCTTAGTTGTGTGCCTGTTGCATTCCTCAAAGCATTTACCTCGCTCGTGAAAACTATAGGAGACGCACCAGATTCTAGTTTGCCTCCTTACTTTAGGTTCTTACCTGTCCAGAACACCCCCTATGATGAATTGAATGTTGTGAGGGAGTCAATCAAAGCACTGCTTATTGAGGAAGACATTGTACCAACCAAGTCACACAAGCAGAGGTTCTTCCATAAACCTCGCGAAGTGGGTAGGTTGTTGCCTGCTTTCTGGAATATTTTGAGAAAGGCGAGCGATGAAAGGGTTAGCTTGGGGAACATCTCTTCCCATGGAAGATATGTTTTGTGTGATTCATTTGACAAAGAGGAGTATGATCACATTCTGAATTTCCTTGGAGTTGAACCAGTTCACGATGAGTGGTATGCTAAATGCATTCAGAGTTCCGATATTGTTGCCGGAGTGTCTGAAGATGTCTATTTGGAGCTTCTATTGTTTGTTTCCGATAACTGGAGGCGGACATTCCACTGTACCAACATCAAGAACATTCCACTGATAAAATATGTGGATTTTGACGGGAGTGTAGCATTCTGCAGCTTAAGTGCAATGCGGATTGGTGAAAAGAAGATTTGCCTATCCCGTCTTTCTTGTCATGTCTCATGGTTGATTGATTGGAACAAGGAATTTATATCCACAGCAAAACTTCTTTTTATGCCAGAAATAACCCAGGAAGCTCTCCAGTCATGTTCTAAGCAGGAGGAGTTGCTGAAATGGTTCGCAGAAGAAATGAAGGTTGCTGCTGTAAGTGTTTACGAGTATGCAGTTTGCCTCGATCATTCTCTTGGCAATGAGCGGAAACCTGTCGTTGCATATGCCCACTTTTTGTATCATTCATTGTGCAATAAGTATATCCCTGCTTACGAAGTTGATGATTTATGTAGTAAAATGCCACTTGTGAATAACTACGGCTACGTTAATAGACAAAGGAGAGGGCTTATTGTCCCTGCCTATGAAAGCAAATGGGCTGATTTGACTGATTCAAATCTGTGGATGGAAGAAGGCTACGTCGAGTTAGGAGAAGATTACATAAAACCAGGTCACTTTGCAGGTAAAGTTACAAAAGAGAAGCGGCTCCTTGAGTTCCTCAAAGATCATGCCGCAGCTTCGGATGTCCCCTCTATTTCTGCTCCCAATGCATGTATTCCAACTGTGTCTTCAACTCTGACGAAGCAAAATGCATTCTTGCTTTTGGACTGGATTCGTCACCTGAAACATGAGAGGGTTCACATTCCAGAGAAGTTTTTGAAAAGCATAGCGAATGGTTACTGGCTGAAAGTTTATTTGAATGGATATTCGGGTGCCATTAGGCCACCTTCGCAGTCATTCATACTCACATCATCATGTGGAAACATTCTGCAGAGTGGATCTAATTTTGTTGATATCCCATTGGTTGACATGAGTTATTATGGGGAGAAAATTAATGATTACAAGGAGGAGCTAAAAGCAGTTGGAGTCATGTTTGAGTACGCAGAAGCATGCAAGTTTATTGGGGACCGTCTTATGTCTCTCGCAGATTCATCCACTTTAACTATAGACAGTGTACTTTCAATCCTGAAATTTATCAAGTTTTTGAGGGACAACTATTTTTCAGCAGAAGAATTCGTATCCAGTATTAAAAGGGGGAGTTGGCTTAAAACGTCCCATGGCTACAGGTCTCCAGTTGGATCTGTCTTGTTCAACCAAGGGTGGCAAATTGCATCCAAAATTAGTGACATTCCATTCATTGATCAGGAGCTTTACGGTGAAGAAATTCTTCATTTCAGAGAAGAACTCGAGTTGCTTGGTGTGGTTGTTTCCTTCCGCACAAATTACCAACTCATGATCGACCACCTAAAGCCACCGTCATGCTTAGCTTCTCTAACATCGGATGCTATTCTACTATTGCTGATAATTATGCAGATCTCAAATTCATCTGATAAGATTGTCGAAACATTGAGTAGAACAAGGTGCCTGAAAGCAAACAATGTTTACAAGTTTCCACATGAATGTCTCTTGGTTCATAAGGAATGGGGTTGCCTTCTTCAGGTGTTTAGTGGTCTCCCATTGATCGATCACAATTTCTATGGAGACAACATATTTTCCTACAGAAATGAGTTGAAGAAAATAGGGGTGGTTGTTGATTATGAGGAGGCTGCAAAAGTATTTGCTCGTTATTTCAAGCATTACGCATCTTCGACTTCCATCACCAAGGAAAATGTTGCATCATTTCTGTTGTGTTGTAGAAAGCTGAAGGGAACTCCTTTTAAATTTCCTGAAGATCTGAAAAGCTGCATTCGGGAGGAAAAGTGGCTGCGCACTCGTCGGGGTGATTATAGATCTCCAAGAGAGTGCATTCTCTTTAGTCCTGATTGGGAATACATCTCTCCAATATCTCGTCTCCCTTTTATCGACGATTCTGAAAATTATTATGGCAAGAATATTCATGAATATAAGAAGGAGCTCAAGAGCATGGGGGTCGCTGTGGAATTCAAGGATGGTGTCAAGTTTGTGCCTCCTAATGTTTGTCTTACCCAGAATCCAAGGAGCATTTCCCCAGAAAATGTGCTAGCATTGCTGGAATGCATGCATATTTTATTGGAGGTGAAAGATTACTCCTTTTCAGATGCATTTATAAAGAGAGTTTCTCAACCATGGTTGAAGACTTATGCTGGTTATAGGCCTCCGAGCGAGTGCTTGTTGTTCGACTCTAAATTTGATTTGTTTCTGAAAAAGACTGACGGGCCTTTCATTGACGAAGAATTTTATGGTTCTAAAATTACAACTTACAGAAAAGAGCTCTCTGAAATAGGGGTGATCGTTGAAGTGGAACAAGGATGTCCATTGATTGCCAGCCACCTTGATTTTCATGATGAACGTTCTACTTTCGTTCGAGTATATGAGTACTTGAATGTGTTTATGTGGAAGCCGGATTGCGAGGCTGACAGAAGGATCTGGATTCCGAATAGAAATCAGAATGGAGCGTGGGCTAGTCCTGATCAATGCGTAATCAATGACAAAGACGGGCTATTTGGTTTGCAGCTGACTGTCCTGGAGACCTACTTTGAGCATAACCTGCTAGCGTTCTTTTCGTATGCTTTTGGGGTAAAATCTCGTCCTTCTATTGAGGACTACTGCAAGCTCTGGAAGGTTTGGGAAAGCTCCAAAATAACATTGTCGCATGTGCACTGTTGCAAGTTCTGGGGTTATATTGCAAAGAGCTGGAATTCGAAAACAGAGAAAGTTCTTACTGAGGCCTTGGTGAAACTGCCTGTTAATTCCAGCTCAGATGAAATCTTGTTGCTAAACAAGTCGGATGTGTTTCTTGCTGATGACTTGCTACTCAAGGATCTTTTTGAGCAGTCTTCTCCTCATCCCTTATTTGTTTGGTACCCTCAGCCAAGCTTGCCTGCATTGCCTCGGACTACATTGCTTGATATTTACAAGAAAATTGGTGTTCGTACTATTTCCGAATCTGTACAGAAGGAAGAACTGCCCCTAGAATATGGCATTGAGCAACAACGGGTAATTCCAAGGGATGGTTTGATTGGGAAACCGCTGTTGAAGCTCATTCTCGGTTTTCTAGCTGATCCCGCCTTCAAAATGGAAGCAGAAAGGAGGCATGAAGCTGTGCAAGGCCTTCTCAGTCTTACTGTTGTGGAGACAACCGAAGCAATCAATGTAAGCTATAACTTACCATTGTCTTCCGGAGAGGTTTTGAACGTAAGGGCAAGCCGAATGATACGGTGGGACAAAGAAATGTGTGCATTTTTCACACAGAAAATGGACAGGTCTGGAGGGCAGAAAAGTGTCATTGAGTTTGCCACATATTTCTCTGAAGTAATAACCTCTGGTGTCCTGTGGGAGAACACTGATCATATACCTGCACTTTCAGAGATGATCAAGTTGGCATTCGTGATGGATTTCGATGAGGAAGCAGTTGAGTTCTATATGAAGTCCAAGAATTTGGGAATCTTCGTCGAGGATGAGGAGTTTCTAAACTCTGCCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1705

Amino Acids

195.51

Weight (kDa)

5.9

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 28 - 144 2.9e-13 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3737
Acc16I TGCGCA 1 cut(s) 3419
Acc36I ACCTGC 3 cut(s) 2162, 4185, 4993
Acc65I GGTACC 1 cut(s) 4491
AccB1I GGYRCC 3 cut(s) 2423, 3072, 4491
AccB7I CCANNNNNTGG 1 cut(s) 4640
AccBSI CCGCTC 1 cut(s) 1918
AccII CGCG 3 cut(s) 354, 719, 1311
AccIII TCCGGA 2 cut(s) 634, 4808
AclI AACGTT 1 cut(s) 3979
AclWI GGATC 8 cut(s) 191, 689, 702, 2497, 2797, 4061, 4458, 4685
AcuI CTGAAG 6 cut(s) 102, 1554, 3131, 3374, 3399, 4959
AfiI CCNNNNNNNGG 4 cut(s) 243, 1575, 3148, 4640
AflII CTTAAG 1 cut(s) 1663
AjnI CCWGG 4 cut(s) 224, 1791, 2158, 4152
AjuI GAANNNNNNNTTGG 4 cut(s) 2263, 2295, 3637, 3669
AloI GAACNNNNNNTCC 4 cut(s) 290, 322, 1376, 1408
Alw21I GWGCWC 4 cut(s) 21, 3567, 3903, 4290
Alw44I GTGCAC 1 cut(s) 4286
AlwI GGATC 8 cut(s) 191, 689, 702, 2497, 2797, 4061, 4458, 4685
Ama87I CYCGRG 3 cut(s) 476, 962, 2897
Aor13HI TCCGGA 2 cut(s) 634, 4808
AoxI GGCC 6 cut(s) 410, 2432, 3782, 3845, 4356, 4738
ApaLI GTGCAC 1 cut(s) 4286
ArsI GACNNNNNNTTYG 4 cut(s) 3800, 3832, 4115, 4147
AseI ATTAAT 1 cut(s) 2544
Asp700I GAANNNNTTC 7 cut(s) 44, 87, 156, 1473, 1838, 2874, 2877
Asp718I GGTACC 1 cut(s) 4491
AspLEI GCGC 5 cut(s) 354, 719, 754, 939, 3420
AspS9I GGNCC 4 cut(s) 327, 598, 2617, 3845
AsuC2I CCSGG 2 cut(s) 963, 964
AsuII TTCGAA 1 cut(s) 4331
AsuNHI GCTAGC 3 cut(s) 916, 3670, 4180
AvaI CYCGRG 3 cut(s) 476, 962, 2897
AvaII GGWCC 3 cut(s) 327, 598, 2617
BaeGI GKGCMC 1 cut(s) 4290
BamHI GGATCC 1 cut(s) 694
BanI GGYRCC 3 cut(s) 2423, 3072, 4491
BanII GRGCYC 3 cut(s) 250, 3567, 3903
BarI GAAGNNNNNNTAC 4 cut(s) 341, 373, 1251, 1283
BauI CACGAG 1 cut(s) 1130
BbsI GAAGAC 6 cut(s) 177, 1266, 2271, 3770, 4458, 4797
Bbv12I GWGCWC 4 cut(s) 21, 3567, 3903, 4290
BbvCI CCTCAGC 1 cut(s) 4497
BccI CCATC 7 cut(s) 59, 609, 2110, 3314, 3593, 4634, 5014
BceAI ACGGC 2 cut(s) 205, 2047
BcgI CGANNNNNNTGC 6 cut(s) 1107, 1141, 1502, 1536, 3562, 3596
BciT130I CCWGG 4 cut(s) 226, 1793, 2160, 4154
BciVI GTATCC 1 cut(s) 2740
BclI TGATCA 7 cut(s) 399, 909, 1441, 2851, 4099, 4975, 5001
BcnI CCSGG 2 cut(s) 963, 964
BfaI CTAG 7 cut(s) 917, 1161, 3671, 4092, 4181, 4601, 4685
BfmI CTRYAG 7 cut(s) 795, 1140, 1657, 2480, 2655, 2773, 3208
BfoI RGCGCY 1 cut(s) 940
BfrI CTTAAG 1 cut(s) 1663
BfuAI ACCTGC 3 cut(s) 2162, 4185, 4993
BfuI GTATCC 1 cut(s) 2740
BglII AGATCT 4 cut(s) 85, 3024, 3382, 3440
BlpI GCTNAGC 1 cut(s) 2974
BmcAI AGTACT 1 cut(s) 4007
Bme1390I CCNGG 6 cut(s) 226, 963, 964, 1793, 2160, 4154
Bme18I GGWCC 3 cut(s) 327, 598, 2617
BmeT110I CYCGRG 3 cut(s) 476, 962, 2897
BmgT120I GGNCC 4 cut(s) 327, 598, 2617, 3845
BmiI GGNNCC 7 cut(s) 249, 696, 2198, 2425, 2618, 3074, 4493
BmrFI CCNGG 6 cut(s) 226, 963, 964, 1793, 2160, 4154
BmsI GCATC 7 cut(s) 640, 757, 2831, 2983, 3302, 3335, 3718
BmtI GCTAGC 3 cut(s) 920, 3674, 4184
BpiI GAAGAC 6 cut(s) 177, 1266, 2271, 3770, 4458, 4797
BplI GAGNNNNNCTC 4 cut(s) 2880, 2912, 3733, 3765
BpmI CTGGAG 5 cut(s) 1597, 1787, 2766, 4175, 4917
Bpu10I CCTNAGC 1 cut(s) 4497
Bpu1102I GCTNAGC 1 cut(s) 2974
Bpu14I TTCGAA 1 cut(s) 4331
BpuEI CTTGAG 4 cut(s) 607, 2222, 3551, 4430
BpuMI CCSGG 2 cut(s) 963, 964
Bsa29I ATCGAT 1 cut(s) 3174
BsaAI YACGTR 1 cut(s) 293
BsaBI GATNNNNATC 2 cut(s) 90, 2106
BsaI GGTCTC 3 cut(s) 2784, 3167, 4151
BsaWI WCCGGW 3 cut(s) 345, 634, 4808
BsaXI ACNNNNNCTCC 4 cut(s) 232, 262, 3395, 3425
Bsc4I CCNNNNNNNGG 4 cut(s) 243, 1575, 3148, 4640
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 7 cut(s) 1472, 1580, 1804, 2324, 2396, 2733, 2783
Bse3DI GCAATG 7 cut(s) 257, 466, 1677, 1918, 3674, 4514, 4529
Bse8I GATNNNNATC 2 cut(s) 90, 2106
BseAI TCCGGA 2 cut(s) 634, 4808
BseBI CCWGG 4 cut(s) 226, 1793, 2160, 4154
BseCI ATCGAT 1 cut(s) 3174
BseJI GATNNNNATC 2 cut(s) 90, 2106
BseLI CCNNNNNNNGG 4 cut(s) 243, 1575, 3148, 4640
BseMI GCAATG 7 cut(s) 257, 466, 1677, 1918, 3674, 4514, 4529
BseMII CTCAG 6 cut(s) 785, 1001, 4344, 4401, 4511, 4759
BseNI ACTGG 7 cut(s) 1472, 1580, 1804, 2324, 2396, 2733, 2783
BseRI GAGGAG 8 cut(s) 893, 898, 1448, 1838, 2573, 3266, 4461, 5105
BseSI GKGCMC 1 cut(s) 4290
BsgI GTGCAG 1 cut(s) 4971
Bsh1236I CGCG 3 cut(s) 354, 719, 1311
BshFI GGCC 6 cut(s) 412, 2434, 3784, 3847, 4358, 4740
BshNI GGYRCC 3 cut(s) 2423, 3072, 4491
BshVI ATCGAT 1 cut(s) 3174
BsiHKAI GWGCWC 4 cut(s) 21, 3567, 3903, 4290
BsiHKCI CYCGRG 3 cut(s) 476, 962, 2897
BsiSI CCGG 7 cut(s) 237, 346, 635, 963, 1524, 4031, 4809
BslFI GGGAC 6 cut(s) 2051, 2222, 2630, 2714, 2749, 4865
BslI CCNNNNNNNGG 4 cut(s) 243, 1575, 3148, 4640
BsmBI CGTCTC 2 cut(s) 1140, 3506
BsmFI GGGAC 6 cut(s) 2051, 2222, 2630, 2714, 2749, 4865
BsnI GGCC 6 cut(s) 412, 2434, 3784, 3847, 4358, 4740
Bso31I GGTCTC 3 cut(s) 2784, 3167, 4151
BsoBI CYCGRG 3 cut(s) 476, 962, 2897
Bsp119I TTCGAA 1 cut(s) 4331
Bsp1286I GDGCHC 5 cut(s) 21, 250, 3567, 3903, 4290
Bsp13I TCCGGA 2 cut(s) 634, 4808
Bsp1407I TGTACA 1 cut(s) 4579
Bsp1720I GCTNAGC 1 cut(s) 2974
Bsp19I CCATGG 3 cut(s) 1396, 2767, 3755
Bsp68I TCGCGA 1 cut(s) 1311
BspANI GGCC 6 cut(s) 412, 2434, 3784, 3847, 4358, 4740
BspCNI CTCAG 6 cut(s) 784, 1000, 4345, 4400, 4510, 4758
BspDI ATCGAT 1 cut(s) 3174
BspEI TCCGGA 2 cut(s) 634, 4808
BspFNI CGCG 3 cut(s) 354, 719, 1311
BspHI TCATGA 3 cut(s) 2943, 3547, 3970
BspLI GGNNCC 7 cut(s) 249, 696, 2198, 2425, 2618, 3074, 4493
BspMAI CTGCAG 3 cut(s) 799, 1661, 2484
BspMI ACCTGC 3 cut(s) 2162, 4185, 4993
BspOI GCTAGC 3 cut(s) 920, 3674, 4184
BspPI GGATC 8 cut(s) 191, 689, 702, 2497, 2797, 4061, 4458, 4685
BspT104I TTCGAA 1 cut(s) 4331
BspT107I GGYRCC 3 cut(s) 2423, 3072, 4491
BspTI CTTAAG 1 cut(s) 1663
BspTNI GGTCTC 3 cut(s) 2784, 3167, 4151
BsrBI CCGCTC 1 cut(s) 1918
BsrDI GCAATG 7 cut(s) 257, 466, 1677, 1918, 3674, 4514, 4529
BsrFI RCCGGY 1 cut(s) 236
BsrGI TGTACA 1 cut(s) 4579
BsrI ACTGG 7 cut(s) 1472, 1580, 1804, 2324, 2396, 2733, 2783
BssAI RCCGGY 1 cut(s) 236
BssSI CACGAG 1 cut(s) 1130
Bst2BI CACGAG 1 cut(s) 1130
Bst2UI CCWGG 4 cut(s) 226, 1793, 2160, 4154
Bst6I CTCTTC 1 cut(s) 1396
BstAFI CTTAAG 1 cut(s) 1663
BstAPI GCANNNNNTGC 1 cut(s) 4177
BstAUI TGTACA 1 cut(s) 4579
BstBAI YACGTR 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 4331
BstDEI CTNAG 9 cut(s) 771, 987, 1088, 1815, 2974, 4353, 4387, 4497, 4745
BstDSI CCRYGG 3 cut(s) 1396, 2767, 3755
BstENI CCTNNNNNAGG 1 cut(s) 3146
BstFNI CGCG 3 cut(s) 354, 719, 1311
BstH2I RGCGCY 1 cut(s) 940
BstHHI GCGC 5 cut(s) 354, 719, 754, 939, 3420
BstNI CCWGG 4 cut(s) 226, 1793, 2160, 4154
BstNSI RCATGY 4 cut(s) 2265, 2604, 3691, 4286
BstSCI CCNGG 6 cut(s) 224, 961, 962, 1791, 2158, 4152
BstSFI CTRYAG 7 cut(s) 795, 1140, 1657, 2480, 2655, 2773, 3208
BstSLI GKGCMC 1 cut(s) 4290
BstUI CGCG 3 cut(s) 354, 719, 1311
BstV2I GAAGAC 6 cut(s) 177, 1266, 2271, 3770, 4458, 4797
BstX2I RGATCY 9 cut(s) 85, 694, 2489, 2789, 3024, 3382, 3440, 4053, 4450
BstXI CCANNNNNNTGG 1 cut(s) 5068
BstYI RGATCY 9 cut(s) 85, 694, 2489, 2789, 3024, 3382, 3440, 4053, 4450
Bsu15I ATCGAT 1 cut(s) 3174
BsuI GTATCC 1 cut(s) 2740
BsuRI GGCC 6 cut(s) 412, 2434, 3784, 3847, 4358, 4740
BsuTUI ATCGAT 1 cut(s) 3174
BtgI CCRYGG 3 cut(s) 1396, 2767, 3755
BtgZI GCGATG 1 cut(s) 1377
BtsI GCAGTG 1 cut(s) 1244
BtsIMutI CAGTG 7 cut(s) 708, 1244, 1591, 1616, 2668, 4288, 4971
BtuMI TCGCGA 1 cut(s) 1311
BveI ACCTGC 3 cut(s) 2162, 4185, 4993
CciI TCATGA 3 cut(s) 2943, 3547, 3970
CfoI GCGC 5 cut(s) 354, 719, 754, 939, 3420
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 4 cut(s) 327, 598, 2617, 3845
Cfr9I CCCGGG 1 cut(s) 962
ClaI ATCGAT 1 cut(s) 3174
CseI GACGC 1 cut(s) 1157
CsiI ACCWGGT 1 cut(s) 2158
DdeI CTNAG 9 cut(s) 771, 987, 1088, 1815, 2974, 4353, 4387, 4497, 4745
DraI TTTAAA 1 cut(s) 3370
Eam1104I CTCTTC 1 cut(s) 1396
EarI CTCTTC 1 cut(s) 1396
EciI GGCGGA 1 cut(s) 1597
Ecl136II GAGCTC 2 cut(s) 3565, 3901
Eco147I AGGCCT 3 cut(s) 3784, 4358, 4740
Eco24I GRGCYC 3 cut(s) 250, 3567, 3903
Eco31I GGTCTC 3 cut(s) 2784, 3167, 4151
Eco32I GATATC 2 cut(s) 723, 2506
Eco47I GGWCC 3 cut(s) 327, 598, 2617
Eco53kI GAGCTC 2 cut(s) 3565, 3901
Eco57I CTGAAG 6 cut(s) 102, 1554, 3131, 3374, 3399, 4959
Eco88I CYCGRG 3 cut(s) 476, 962, 2897
EcoICRI GAGCTC 2 cut(s) 3565, 3901
EcoNI CCTNNNNNAGG 1 cut(s) 3146
EcoRI GAATTC 4 cut(s) 1083, 2723, 3590, 4327
EcoRII CCWGG 4 cut(s) 224, 1791, 2158, 4152
EcoRV GATATC 2 cut(s) 723, 2506
EcoT22I ATGCAT 8 cut(s) 633, 976, 1502, 2263, 2305, 3689, 3693, 3733
EcoT38I GRGCYC 3 cut(s) 250, 3567, 3903
Esp3I CGTCTC 2 cut(s) 1140, 3506
FalI AAGNNNNNCTT 4 cut(s) 1233, 1265, 2862, 2894
FaqI GGGAC 6 cut(s) 2051, 2222, 2630, 2714, 2749, 4865
FauI CCCGC 1 cut(s) 4702
FauNDI CATATG 2 cut(s) 928, 1936
FbaI TGATCA 7 cut(s) 399, 909, 1441, 2851, 4099, 4975, 5001
FriOI GRGCYC 3 cut(s) 250, 3567, 3903
FspBI CTAG 7 cut(s) 917, 1161, 3671, 4092, 4181, 4601, 4685
FspI TGCGCA 1 cut(s) 3419
GlaI GCGC 5 cut(s) 353, 718, 753, 938, 3419
GsuI CTGGAG 5 cut(s) 1597, 1787, 2766, 4175, 4917
HaeII RGCGCY 1 cut(s) 940
HaeIII GGCC 6 cut(s) 412, 2434, 3784, 3847, 4358, 4740
HapII CCGG 7 cut(s) 237, 346, 635, 963, 1524, 4031, 4809
HgaI GACGC 1 cut(s) 1157
HhaI GCGC 5 cut(s) 354, 719, 754, 939, 3420
Hin6I GCGC 5 cut(s) 352, 717, 752, 937, 3418
HinP1I GCGC 5 cut(s) 352, 717, 752, 937, 3418
HincII GTYRAC 1 cut(s) 2518
HindII GTYRAC 1 cut(s) 2518
HindIII AAGCTT 1 cut(s) 4504
HpaII CCGG 7 cut(s) 237, 346, 635, 963, 1524, 4031, 4809
Hpy99I CGWCG 4 cut(s) 2135, 3429, 3518, 5084
HpyCH4IV ACGT 7 cut(s) 221, 292, 2037, 2130, 2762, 3979, 4824
HpyF3I CTNAG 9 cut(s) 771, 987, 1088, 1815, 2974, 4353, 4387, 4497, 4745
HpySE526I ACGT 7 cut(s) 221, 292, 2037, 2130, 2762, 3979, 4824
HspAI GCGC 5 cut(s) 352, 717, 752, 937, 3418
Kpn2I TCCGGA 2 cut(s) 634, 4808
KpnI GGTACC 1 cut(s) 4495
Ksp22I TGATCA 7 cut(s) 399, 909, 1441, 2851, 4099, 4975, 5001
LweI GCATC 7 cut(s) 640, 757, 2831, 2983, 3302, 3335, 3718
MabI ACCWGGT 1 cut(s) 2158
MaeI CTAG 7 cut(s) 917, 1161, 3671, 4092, 4181, 4601, 4685
MaeII ACGT 7 cut(s) 221, 292, 2037, 2130, 2762, 3979, 4824
MbiI CCGCTC 1 cut(s) 1918
MflI RGATCY 9 cut(s) 85, 694, 2489, 2789, 3024, 3382, 3440, 4053, 4450
MhlI GDGCHC 5 cut(s) 21, 250, 3567, 3903, 4290
MlyI GAGTC 3 cut(s) 1241, 2586, 3803
MmeI TCCRAC 6 cut(s) 584, 1007, 2294, 2554, 2767, 4395
Mph1103I ATGCAT 8 cut(s) 633, 976, 1502, 2263, 2305, 3689, 3693, 3733
MroI TCCGGA 2 cut(s) 634, 4808
MroXI GAANNNNTTC 7 cut(s) 44, 87, 156, 1473, 1838, 2874, 2877
MslI CAYNNNNRTG 3 cut(s) 2464, 2968, 3109
MspA1I CMGCKG 2 cut(s) 4144, 4660
MspCI CTTAAG 1 cut(s) 1663
MspI CCGG 7 cut(s) 237, 346, 635, 963, 1524, 4031, 4809
MspR9I CCNGG 6 cut(s) 226, 963, 964, 1793, 2160, 4154
MvaI CCWGG 4 cut(s) 226, 1793, 2160, 4154
MvnI CGCG 3 cut(s) 354, 719, 1311
NciI CCSGG 2 cut(s) 963, 964
NcoI CCATGG 3 cut(s) 1396, 2767, 3755
NdeI CATATG 2 cut(s) 928, 1936
NheI GCTAGC 3 cut(s) 916, 3670, 4180
NlaIV GGNNCC 7 cut(s) 249, 696, 2198, 2425, 2618, 3074, 4493
NmuCI GTSAC 6 cut(s) 201, 521, 1278, 2162, 2327, 2834
NruI TCGCGA 1 cut(s) 1311
NsbI TGCGCA 1 cut(s) 3419
NsiI ATGCAT 8 cut(s) 633, 976, 1502, 2263, 2305, 3689, 3693, 3733
NspI RCATGY 4 cut(s) 2265, 2604, 3691, 4286
NspV TTCGAA 1 cut(s) 4331
PaeI GCATGC 2 cut(s) 2604, 3691
PaeR7I CTCGAG 1 cut(s) 2897
PagI TCATGA 3 cut(s) 2943, 3547, 3970
PceI AGGCCT 3 cut(s) 3784, 4358, 4740
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PdmI GAANNNNTTC 7 cut(s) 44, 87, 156, 1473, 1838, 2874, 2877
PflFI GACNNNGTC 1 cut(s) 596
PflMI CCANNNNNTGG 1 cut(s) 4640
PfoI TCCNGGA 1 cut(s) 4152
PleI GAGTC 3 cut(s) 1240, 2585, 3803
PpsI GAGTC 3 cut(s) 1240, 2585, 3803
Ppu21I YACGTR 1 cut(s) 293
PshBI ATTAAT 1 cut(s) 2544
PsiI TTATAA 1 cut(s) 3737
Psp124BI GAGCTC 2 cut(s) 3567, 3903
Psp1406I AACGTT 1 cut(s) 3979
Psp6I CCWGG 4 cut(s) 224, 1791, 2158, 4152
PspGI CCWGG 4 cut(s) 224, 1791, 2158, 4152
PspN4I GGNNCC 7 cut(s) 249, 696, 2198, 2425, 2618, 3074, 4493
PspPI GGNCC 4 cut(s) 327, 598, 2617, 3845
PspXI VCTCGAGB 1 cut(s) 2897
PstI CTGCAG 3 cut(s) 799, 1661, 2484
PsuI RGATCY 9 cut(s) 85, 694, 2489, 2789, 3024, 3382, 3440, 4053, 4450
PsyI GACNNNGTC 1 cut(s) 596
PvuII CAGCTG 1 cut(s) 4144
RruI TCGCGA 1 cut(s) 1311
RseI CAYNNNNRTG 3 cut(s) 2464, 2968, 3109
SacI GAGCTC 2 cut(s) 3567, 3903
Sau96I GGNCC 4 cut(s) 327, 598, 2617, 3845
ScaI AGTACT 1 cut(s) 4007
SchI GAGTC 3 cut(s) 1241, 2586, 3803
ScrFI CCNGG 6 cut(s) 226, 963, 964, 1793, 2160, 4154
SduI GDGCHC 5 cut(s) 21, 250, 3567, 3903, 4290
SexAI ACCWGGT 1 cut(s) 2158
SfaNI GCATC 7 cut(s) 640, 757, 2831, 2983, 3302, 3335, 3718
SfcI CTRYAG 7 cut(s) 795, 1140, 1657, 2480, 2655, 2773, 3208
Sfr274I CTCGAG 1 cut(s) 2897
SfuI TTCGAA 1 cut(s) 4331
SinI GGWCC 3 cut(s) 327, 598, 2617
SlaI CTCGAG 1 cut(s) 2897
SmaI CCCGGG 1 cut(s) 964
SmiMI CAYNNNNRTG 3 cut(s) 2464, 2968, 3109
SmlI CTYRAG 6 cut(s) 622, 1663, 2201, 2897, 3566, 4445
SmoI CTYRAG 6 cut(s) 622, 1663, 2201, 2897, 3566, 4445
SphI GCATGC 2 cut(s) 2604, 3691
SseBI AGGCCT 3 cut(s) 3784, 4358, 4740
SspI AATATT 2 cut(s) 1345, 3544
SspMI CTAG 7 cut(s) 917, 1161, 3671, 4092, 4181, 4601, 4685
SstI GAGCTC 2 cut(s) 3567, 3903
StuI AGGCCT 3 cut(s) 3784, 4358, 4740
StyD4I CCNGG 6 cut(s) 224, 961, 962, 1791, 2158, 4152
TaiI ACGT 7 cut(s) 224, 295, 2040, 2133, 2765, 3982, 4827
TatI WGTACW 3 cut(s) 2665, 4005, 4579
TauI GCSGC 4 cut(s) 357, 670, 2197, 2225
TscAI CASTG 7 cut(s) 715, 1251, 1598, 1623, 2668, 4295, 4978
TseFI GTSAC 6 cut(s) 201, 521, 1278, 2162, 2327, 2834
Tsp45I GTSAC 6 cut(s) 201, 521, 1278, 2162, 2327, 2834
TspGWI ACGGA 1 cut(s) 189
TspMI CCCGGG 1 cut(s) 962
TspRI CASTG 7 cut(s) 715, 1251, 1598, 1623, 2668, 4295, 4978
Tth111I GACNNNGTC 1 cut(s) 596
Van91I CCANNNNNTGG 1 cut(s) 4640
Vha464I CTTAAG 1 cut(s) 1663
VneI GTGCAC 1 cut(s) 4286
VpaK11BI GGWCC 3 cut(s) 327, 598, 2617
VspI ATTAAT 1 cut(s) 2544
XagI CCTNNNNNAGG 1 cut(s) 3146
XceI RCATGY 4 cut(s) 2265, 2604, 3691, 4286
XhoI CTCGAG 1 cut(s) 2897
XmaI CCCGGG 1 cut(s) 962
XmnI GAANNNNTTC 7 cut(s) 44, 87, 156, 1473, 1838, 2874, 2877
XspI CTAG 7 cut(s) 917, 1161, 3671, 4092, 4181, 4601, 4685
ZrmI AGTACT 1 cut(s) 4007
Zsp2I ATGCAT 8 cut(s) 633, 976, 1502, 2263, 2305, 3689, 3693, 3733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.